Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB1I | GGYRCC | 3 cut(s) | 170, 1005, 1274 |
| AccII | CGCG | 1 cut(s) | 192 |
| AciI | CCGC | 6 cut(s) | 129, 250, 282, 361, 1026, 1245 |
| AclWI | GGATC | 5 cut(s) | 13, 444, 457, 911, 1245 |
| AcoI | YGGCCR | 2 cut(s) | 100, 906 |
| AfaI | GTAC | 4 cut(s) | 515, 564, 1190, 1316 |
| AfiI | CCNNNNNNNGG | 5 cut(s) | 460, 726, 926, 1075, 1255 |
| AgsI | TTSAA | 4 cut(s) | 148, 415, 992, 1021 |
| AjiI | CACGTC | 1 cut(s) | 949 |
| AjnI | CCWGG | 3 cut(s) | 405, 582, 1057 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 1160, 1192 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 1202, 1234 |
| AluBI | AGCT | 5 cut(s) | 67, 256, 274, 499, 856 |
| AluI | AGCT | 5 cut(s) | 67, 256, 274, 499, 856 |
| Alw21I | GWGCWC | 3 cut(s) | 591, 742, 1185 |
| Alw26I | GTCTC | 1 cut(s) | 129 |
| Alw44I | GTGCAC | 1 cut(s) | 1181 |
| AlwI | GGATC | 5 cut(s) | 13, 444, 457, 911, 1245 |
| Ama87I | CYCGRG | 2 cut(s) | 209, 1250 |
| AoxI | GGCC | 4 cut(s) | 33, 100, 906, 1227 |
| ApaLI | GTGCAC | 1 cut(s) | 1181 |
| ApeKI | GCWGC | 3 cut(s) | 271, 296, 956 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 1133, 1165 |
| AspLEI | GCGC | 3 cut(s) | 194, 645, 984 |
| AspS9I | GGNCC | 2 cut(s) | 358, 1228 |
| AsuC2I | CCSGG | 2 cut(s) | 175, 442 |
| AsuHPI | GGTGA | 4 cut(s) | 865, 1076, 1208, 1226 |
| AvaI | CYCGRG | 2 cut(s) | 209, 1250 |
| AvaII | GGWCC | 1 cut(s) | 358 |
| BaeGI | GKGCMC | 2 cut(s) | 1010, 1185 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 1180, 1213 |
| BalI | TGGCCA | 2 cut(s) | 102, 908 |
| BamHI | GGATCC | 1 cut(s) | 449 |
| BanI | GGYRCC | 3 cut(s) | 170, 1005, 1274 |
| BanII | GRGCYC | 3 cut(s) | 216, 552, 894 |
| BbsI | GAAGAC | 2 cut(s) | 403, 1120 |
| Bbv12I | GWGCWC | 3 cut(s) | 591, 742, 1185 |
| BbvCI | CCTCAGC | 2 cut(s) | 1122, 1287 |
| BbvI | GCAGC | 3 cut(s) | 283, 283, 943 |
| BccI | CCATC | 3 cut(s) | 92, 898, 977 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 170, 204 |
| BciT130I | CCWGG | 3 cut(s) | 407, 584, 1059 |
| BciVI | GTATCC | 1 cut(s) | 98 |
| BcnI | CCSGG | 2 cut(s) | 175, 442 |
| BcoDI | GTCTC | 1 cut(s) | 129 |
| BfmI | CTRYAG | 3 cut(s) | 123, 420, 637 |
| BfoI | RGCGCY | 1 cut(s) | 646 |
| BfuI | GTATCC | 1 cut(s) | 98 |
| BisI | GCNGC | 5 cut(s) | 272, 283, 297, 957, 1245 |
| BlsI | GCNGC | 5 cut(s) | 273, 284, 298, 958, 1246 |
| BmcAI | AGTACT | 1 cut(s) | 1316 |
| Bme1390I | CCNGG | 5 cut(s) | 175, 407, 442, 584, 1059 |
| Bme18I | GGWCC | 1 cut(s) | 358 |
| BmeT110I | CYCGRG | 2 cut(s) | 209, 1250 |
| BmgBI | CACGTC | 1 cut(s) | 949 |
| BmgT120I | GGNCC | 2 cut(s) | 358, 1228 |
| BmiI | GGNNCC | 8 cut(s) | 172, 244, 404, 439, 451, 780, 1007, 1276 |
| BmrFI | CCNGG | 5 cut(s) | 175, 407, 442, 584, 1059 |
| BmsI | GCATC | 3 cut(s) | 130, 283, 372 |
| BpiI | GAAGAC | 2 cut(s) | 403, 1120 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 1115, 1147 |
| BpmI | CTGGAG | 2 cut(s) | 605, 1080 |
| Bpu10I | CCTNAGC | 3 cut(s) | 267, 1122, 1287 |
| BpuEI | CTTGAG | 3 cut(s) | 98, 745, 1117 |
| BpuMI | CCSGG | 2 cut(s) | 175, 442 |
| BsaAI | YACGTR | 1 cut(s) | 1192 |
| BsaBI | GATNNNNATC | 1 cut(s) | 23 |
| BsaJI | CCNNGG | 3 cut(s) | 925, 1057, 1074 |
| BsaWI | WCCGGW | 1 cut(s) | 1013 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 455, 485 |
| Bsc4I | CCNNNNNNNGG | 5 cut(s) | 460, 726, 926, 1075, 1255 |
| Bse118I | RCCGGY | 1 cut(s) | 35 |
| Bse1I | ACTGG | 7 cut(s) | 172, 244, 394, 439, 1007, 1113, 1190 |
| Bse3DI | GCAATG | 1 cut(s) | 58 |
| Bse8I | GATNNNNATC | 1 cut(s) | 23 |
| BseBI | CCWGG | 3 cut(s) | 407, 584, 1059 |
| BseDI | CCNNGG | 3 cut(s) | 925, 1057, 1074 |
| BseGI | GGATG | 2 cut(s) | 757, 1315 |
| BseJI | GATNNNNATC | 1 cut(s) | 23 |
| BseLI | CCNNNNNNNGG | 5 cut(s) | 460, 726, 926, 1075, 1255 |
| BseMI | GCAATG | 1 cut(s) | 58 |
| BseMII | CTCAG | 8 cut(s) | 258, 710, 750, 825, 858, 1113, 1215, 1278 |
| BseNI | ACTGG | 7 cut(s) | 172, 244, 394, 439, 1007, 1113, 1190 |
| BseRI | GAGGAG | 1 cut(s) | 147 |
| BseSI | GKGCMC | 2 cut(s) | 1010, 1185 |
| BseXI | GCAGC | 3 cut(s) | 283, 283, 943 |
| BseYI | CCCAGC | 1 cut(s) | 60 |
| BsgI | GTGCAG | 2 cut(s) | 370, 1100 |
| Bsh1236I | CGCG | 1 cut(s) | 192 |
| BshFI | GGCC | 4 cut(s) | 35, 102, 908, 1229 |
| BshNI | GGYRCC | 3 cut(s) | 170, 1005, 1274 |
| BsiHKAI | GWGCWC | 3 cut(s) | 591, 742, 1185 |
| BsiHKCI | CYCGRG | 2 cut(s) | 209, 1250 |
| BsiSI | CCGG | 4 cut(s) | 36, 174, 441, 1014 |
| BslFI | GGGAC | 2 cut(s) | 170, 1040 |
| BslI | CCNNNNNNNGG | 5 cut(s) | 460, 726, 926, 1075, 1255 |
| BsmAI | GTCTC | 1 cut(s) | 129 |
| BsmBI | CGTCTC | 1 cut(s) | 129 |
| BsmFI | GGGAC | 2 cut(s) | 170, 1040 |
| BsmI | GAATGC | 1 cut(s) | 958 |
| BsnI | GGCC | 4 cut(s) | 35, 102, 908, 1229 |
| BsoBI | CYCGRG | 2 cut(s) | 209, 1250 |
| Bsp1286I | GDGCHC | 7 cut(s) | 216, 552, 591, 742, 894, 1010, 1185 |
| Bsp143I | GATC | 9 cut(s) | 18, 205, 346, 449, 870, 916, 1098, 1153, 1237 |
| Bsp19I | CCATGG | 1 cut(s) | 925 |
| BspACI | CCGC | 6 cut(s) | 129, 250, 282, 361, 1026, 1245 |
| BspANI | GGCC | 4 cut(s) | 35, 102, 908, 1229 |
| BspCNI | CTCAG | 8 cut(s) | 259, 709, 751, 826, 859, 1114, 1216, 1279 |
| BspFNI | CGCG | 1 cut(s) | 192 |
| BspLI | GGNNCC | 8 cut(s) | 172, 244, 404, 439, 451, 780, 1007, 1276 |
| BspMAI | CTGCAG | 1 cut(s) | 641 |
| BspPI | GGATC | 5 cut(s) | 13, 444, 457, 911, 1245 |
| BspQI | GCTCTTC | 1 cut(s) | 263 |
| BspT107I | GGYRCC | 3 cut(s) | 170, 1005, 1274 |
| BsrDI | GCAATG | 1 cut(s) | 58 |
| BsrFI | RCCGGY | 1 cut(s) | 35 |
| BsrI | ACTGG | 7 cut(s) | 172, 244, 394, 439, 1007, 1113, 1190 |
| BssAI | RCCGGY | 1 cut(s) | 35 |
| BssECI | CCNNGG | 3 cut(s) | 925, 1057, 1074 |
| BssMI | GATC | 9 cut(s) | 18, 205, 346, 449, 870, 916, 1098, 1153, 1237 |
| BssT1I | CCWWGG | 2 cut(s) | 925, 1074 |
| Bst2UI | CCWGG | 3 cut(s) | 407, 584, 1059 |
| Bst4CI | ACNGT | 7 cut(s) | 124, 220, 511, 560, 737, 881, 1051 |
| Bst6I | CTCTTC | 2 cut(s) | 263, 1260 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 57 |
| BstBAI | YACGTR | 1 cut(s) | 1192 |
| BstC8I | GCNNGC | 5 cut(s) | 58, 254, 641, 942, 1173 |
| BstDEI | CTNAG | 8 cut(s) | 267, 696, 759, 834, 867, 1122, 1224, 1287 |
| BstDSI | CCRYGG | 1 cut(s) | 925 |
| BstEII | GGTNACC | 1 cut(s) | 1064 |
| BstENI | CCTNNNNNAGG | 3 cut(s) | 724, 1073, 1253 |
| BstF5I | GGATG | 2 cut(s) | 757, 1315 |
| BstFNI | CGCG | 1 cut(s) | 192 |
| BstH2I | RGCGCY | 1 cut(s) | 646 |
| BstHHI | GCGC | 3 cut(s) | 194, 645, 984 |
| BstKTI | GATC | 9 cut(s) | 21, 208, 349, 452, 873, 919, 1101, 1156, 1240 |
| BstMAI | GTCTC | 1 cut(s) | 129 |
| BstMBI | GATC | 9 cut(s) | 18, 205, 346, 449, 870, 916, 1098, 1153, 1237 |
| BstMWI | GCNNNNNNNGC | 7 cut(s) | 57, 360, 839, 921, 962, 1172, 1283 |
| BstNI | CCWGG | 3 cut(s) | 407, 584, 1059 |
| BstNSI | RCATGY | 1 cut(s) | 185 |
| BstPI | GGTNACC | 1 cut(s) | 1064 |
| BstSCI | CCNGG | 5 cut(s) | 173, 405, 440, 582, 1057 |
| BstSFI | CTRYAG | 3 cut(s) | 123, 420, 637 |
| BstSLI | GKGCMC | 2 cut(s) | 1010, 1185 |
| BstUI | CGCG | 1 cut(s) | 192 |
| BstV1I | GCAGC | 3 cut(s) | 283, 283, 943 |
| BstV2I | GAAGAC | 2 cut(s) | 403, 1120 |
| BstX2I | RGATCY | 2 cut(s) | 449, 1237 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 98 |
| BstYI | RGATCY | 2 cut(s) | 449, 1237 |
| BsuI | GTATCC | 1 cut(s) | 98 |
| BsuRI | GGCC | 4 cut(s) | 35, 102, 908, 1229 |
| BtgI | CCRYGG | 1 cut(s) | 925 |
| BtgZI | GCGATG | 1 cut(s) | 1308 |
| BtrI | CACGTC | 1 cut(s) | 949 |
| BtsCI | GGATG | 2 cut(s) | 757, 1315 |
| BtsIMutI | CAGTG | 8 cut(s) | 166, 387, 432, 565, 886, 1047, 1120, 1183 |
| Cac8I | GCNNGC | 5 cut(s) | 58, 254, 641, 942, 1173 |
| CfoI | GCGC | 3 cut(s) | 194, 645, 984 |
| Cfr10I | RCCGGY | 1 cut(s) | 35 |
| Cfr13I | GGNCC | 2 cut(s) | 358, 1228 |
| Csp6I | GTAC | 4 cut(s) | 514, 563, 1189, 1315 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 205, 240 |
| CviAII | CATG | 7 cut(s) | 83, 182, 848, 926, 936, 1096, 1159 |
| CviQI | GTAC | 4 cut(s) | 514, 563, 1189, 1315 |
| DdeI | CTNAG | 8 cut(s) | 267, 696, 759, 834, 867, 1122, 1224, 1287 |
| DpnI | GATC | 9 cut(s) | 20, 207, 348, 451, 872, 918, 1100, 1155, 1239 |
| DpnII | GATC | 9 cut(s) | 18, 205, 346, 449, 870, 916, 1098, 1153, 1237 |
| EaeI | YGGCCR | 2 cut(s) | 100, 906 |
| Eam1104I | CTCTTC | 2 cut(s) | 263, 1260 |
| EarI | CTCTTC | 2 cut(s) | 263, 1260 |
| EciI | GGCGGA | 1 cut(s) | 144 |
| Eco130I | CCWWGG | 2 cut(s) | 925, 1074 |
| Eco24I | GRGCYC | 3 cut(s) | 216, 552, 894 |
| Eco32I | GATATC | 1 cut(s) | 634 |
| Eco47I | GGWCC | 1 cut(s) | 358 |
| Eco88I | CYCGRG | 2 cut(s) | 209, 1250 |
| Eco91I | GGTNACC | 1 cut(s) | 1064 |
| EcoNI | CCTNNNNNAGG | 3 cut(s) | 724, 1073, 1253 |
| EcoO65I | GGTNACC | 1 cut(s) | 1064 |
| EcoRII | CCWGG | 3 cut(s) | 405, 582, 1057 |
| EcoRV | GATATC | 1 cut(s) | 634 |
| EcoT14I | CCWWGG | 2 cut(s) | 925, 1074 |
| EcoT38I | GRGCYC | 3 cut(s) | 216, 552, 894 |
| ErhI | CCWWGG | 2 cut(s) | 925, 1074 |
| Esp3I | CGTCTC | 1 cut(s) | 129 |
| FaeI | CATG | 7 cut(s) | 86, 185, 851, 929, 939, 1099, 1162 |
| FaqI | GGGAC | 2 cut(s) | 170, 1040 |
| FatI | CATG | 7 cut(s) | 82, 181, 847, 925, 935, 1095, 1158 |
| Fnu4HI | GCNGC | 5 cut(s) | 272, 283, 297, 957, 1245 |
| FokI | GGATG | 1 cut(s) | 764 |
| FriOI | GRGCYC | 3 cut(s) | 216, 552, 894 |
| Fsp4HI | GCNGC | 5 cut(s) | 272, 283, 297, 957, 1245 |
| GlaI | GCGC | 3 cut(s) | 193, 644, 983 |
| GluI | GCNGC | 5 cut(s) | 272, 283, 297, 957, 1245 |
| GsaI | CCCAGC | 1 cut(s) | 64 |
| GsuI | CTGGAG | 2 cut(s) | 605, 1080 |
| HaeII | RGCGCY | 1 cut(s) | 646 |
| HaeIII | GGCC | 4 cut(s) | 35, 102, 908, 1229 |
| HapII | CCGG | 4 cut(s) | 36, 174, 441, 1014 |
| HhaI | GCGC | 3 cut(s) | 194, 645, 984 |
| Hin1II | CATG | 7 cut(s) | 86, 185, 851, 929, 939, 1099, 1162 |
| Hin6I | GCGC | 3 cut(s) | 192, 643, 982 |
| HinP1I | GCGC | 3 cut(s) | 192, 643, 982 |
| HincII | GTYRAC | 2 cut(s) | 478, 1000 |
| HindII | GTYRAC | 2 cut(s) | 478, 1000 |
| HindIII | AAGCTT | 1 cut(s) | 497 |
| HinfI | GANTC | 3 cut(s) | 792, 1017, 1280 |
| HpaII | CCGG | 4 cut(s) | 36, 174, 441, 1014 |
| HphI | GGTGA | 4 cut(s) | 865, 1076, 1208, 1226 |
| Hpy166II | GTNNAC | 6 cut(s) | 478, 507, 514, 563, 1000, 1183 |
| Hpy188I | TCNGA | 1 cut(s) | 1225 |
| Hpy188III | TCNNGA | 7 cut(s) | 209, 367, 650, 766, 973, 1134, 1252 |
| Hpy8I | GTNNAC | 6 cut(s) | 478, 507, 514, 563, 1000, 1183 |
| HpyAV | CCTTC | 6 cut(s) | 154, 464, 832, 1205, 1249, 1298 |
| HpyCH4III | ACNGT | 7 cut(s) | 124, 220, 511, 560, 737, 881, 1051 |
| HpyCH4IV | ACGT | 2 cut(s) | 948, 1191 |
| HpyCH4V | TGCA | 8 cut(s) | 12, 387, 397, 639, 818, 1081, 1095, 1183 |
| HpyF10VI | GCNNNNNNNGC | 7 cut(s) | 57, 360, 839, 921, 962, 1172, 1283 |
| HpyF3I | CTNAG | 8 cut(s) | 267, 696, 759, 834, 867, 1122, 1224, 1287 |
| HpySE526I | ACGT | 2 cut(s) | 948, 1191 |
| Hsp92II | CATG | 7 cut(s) | 86, 185, 851, 929, 939, 1099, 1162 |
| HspAI | GCGC | 3 cut(s) | 192, 643, 982 |
| Kzo9I | GATC | 9 cut(s) | 18, 205, 346, 449, 870, 916, 1098, 1153, 1237 |
| LguI | GCTCTTC | 1 cut(s) | 263 |
| LmnI | GCTCC | 4 cut(s) | 64, 443, 463, 586 |
| Lsp1109I | GCAGC | 3 cut(s) | 283, 283, 943 |
| LweI | GCATC | 3 cut(s) | 130, 283, 372 |
| MaeII | ACGT | 2 cut(s) | 948, 1191 |
| MaeIII | GTNAC | 4 cut(s) | 113, 317, 754, 1064 |
| MalI | GATC | 9 cut(s) | 20, 207, 348, 451, 872, 918, 1100, 1155, 1239 |
| MboI | GATC | 9 cut(s) | 18, 205, 346, 449, 870, 916, 1098, 1153, 1237 |
| MboII | GAAGA | 6 cut(s) | 250, 302, 403, 801, 1120, 1277 |
| MflI | RGATCY | 2 cut(s) | 449, 1237 |
| MhlI | GDGCHC | 7 cut(s) | 216, 552, 591, 742, 894, 1010, 1185 |
| MlsI | TGGCCA | 2 cut(s) | 102, 908 |
| MluCI | AATT | 3 cut(s) | 987, 992, 1137 |
| MluNI | TGGCCA | 2 cut(s) | 102, 908 |
| MlyI | GAGTC | 1 cut(s) | 1289 |
| MmeI | TCCRAC | 2 cut(s) | 441, 1128 |
| Mox20I | TGGCCA | 2 cut(s) | 102, 908 |
| MscI | TGGCCA | 2 cut(s) | 102, 908 |
| MslI | CAYNNNNRTG | 1 cut(s) | 594 |
| Msp20I | TGGCCA | 2 cut(s) | 102, 908 |
| MspA1I | CMGCKG | 1 cut(s) | 282 |
| MspI | CCGG | 4 cut(s) | 36, 174, 441, 1014 |
| MspR9I | CCNGG | 5 cut(s) | 175, 407, 442, 584, 1059 |
| Mva1269I | GAATGC | 1 cut(s) | 958 |
| MvaI | CCWGG | 3 cut(s) | 407, 584, 1059 |
| MvnI | CGCG | 1 cut(s) | 192 |
| MwoI | GCNNNNNNNGC | 7 cut(s) | 57, 360, 839, 921, 962, 1172, 1283 |
| NciI | CCSGG | 2 cut(s) | 175, 442 |
| NcoI | CCATGG | 1 cut(s) | 925 |
| NdeII | GATC | 9 cut(s) | 18, 205, 346, 449, 870, 916, 1098, 1153, 1237 |
| NlaIII | CATG | 7 cut(s) | 86, 185, 851, 929, 939, 1099, 1162 |
| NlaIV | GGNNCC | 8 cut(s) | 172, 244, 404, 439, 451, 780, 1007, 1276 |
| NmeAIII | GCCGAG | 1 cut(s) | 1303 |
| NmuCI | GTSAC | 3 cut(s) | 113, 317, 1064 |
| NspI | RCATGY | 1 cut(s) | 185 |
| PaeR7I | CTCGAG | 2 cut(s) | 209, 1250 |
| PciSI | GCTCTTC | 1 cut(s) | 263 |
| PctI | GAATGC | 1 cut(s) | 958 |
| PfeI | GAWTC | 2 cut(s) | 792, 1017 |
| PfoI | TCCNGGA | 1 cut(s) | 582 |
| PkrI | GCNGC | 5 cut(s) | 273, 284, 298, 958, 1246 |
| PleI | GAGTC | 1 cut(s) | 1288 |
| PpsI | GAGTC | 1 cut(s) | 1288 |
| Ppu21I | YACGTR | 1 cut(s) | 1192 |
| Psp6I | CCWGG | 3 cut(s) | 405, 582, 1057 |
| PspEI | GGTNACC | 1 cut(s) | 1064 |
| PspFI | CCCAGC | 1 cut(s) | 60 |
| PspGI | CCWGG | 3 cut(s) | 405, 582, 1057 |
| PspN4I | GGNNCC | 8 cut(s) | 172, 244, 404, 439, 451, 780, 1007, 1276 |
| PspPI | GGNCC | 2 cut(s) | 358, 1228 |
| PstI | CTGCAG | 1 cut(s) | 641 |
| PsuI | RGATCY | 2 cut(s) | 449, 1237 |
| RsaI | GTAC | 4 cut(s) | 515, 564, 1190, 1316 |
| RsaNI | GTAC | 4 cut(s) | 514, 563, 1189, 1315 |
| RseI | CAYNNNNRTG | 1 cut(s) | 594 |
| SapI | GCTCTTC | 1 cut(s) | 263 |
| SatI | GCNGC | 5 cut(s) | 272, 283, 297, 957, 1245 |
| Sau3AI | GATC | 9 cut(s) | 18, 205, 346, 449, 870, 916, 1098, 1153, 1237 |
| Sau96I | GGNCC | 2 cut(s) | 358, 1228 |
| ScaI | AGTACT | 1 cut(s) | 1316 |
| SchI | GAGTC | 1 cut(s) | 1289 |
| ScrFI | CCNGG | 5 cut(s) | 175, 407, 442, 584, 1059 |
| SduI | GDGCHC | 7 cut(s) | 216, 552, 591, 742, 894, 1010, 1185 |
| SfaNI | GCATC | 3 cut(s) | 130, 283, 372 |
| SfcI | CTRYAG | 3 cut(s) | 123, 420, 637 |
| Sfr274I | CTCGAG | 2 cut(s) | 209, 1250 |
| SinI | GGWCC | 1 cut(s) | 358 |
| SlaI | CTCGAG | 2 cut(s) | 209, 1250 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 594 |
| SmlI | CTYRAG | 5 cut(s) | 77, 209, 724, 1132, 1250 |
| SmoI | CTYRAG | 5 cut(s) | 77, 209, 724, 1132, 1250 |
| Sse9I | AATT | 3 cut(s) | 987, 992, 1137 |
| SsiI | CCGC | 6 cut(s) | 129, 250, 282, 361, 1026, 1245 |
| StyD4I | CCNGG | 5 cut(s) | 173, 405, 440, 582, 1057 |
| StyI | CCWWGG | 2 cut(s) | 925, 1074 |
| TaaI | ACNGT | 7 cut(s) | 124, 220, 511, 560, 737, 881, 1051 |
| TaiI | ACGT | 2 cut(s) | 951, 1194 |
| TaqI | TCGA | 3 cut(s) | 17, 210, 1251 |
| TaqII | GACCGA | 1 cut(s) | 17 |
| TasI | AATT | 3 cut(s) | 987, 992, 1137 |
| TatI | WGTACW | 2 cut(s) | 562, 1314 |
| TauI | GCSGC | 2 cut(s) | 285, 1247 |
| TfiI | GAWTC | 2 cut(s) | 792, 1017 |
| TscAI | CASTG | 8 cut(s) | 166, 394, 439, 565, 886, 1054, 1120, 1190 |
| TseFI | GTSAC | 3 cut(s) | 113, 317, 1064 |
| TseI | GCWGC | 3 cut(s) | 271, 296, 956 |
| Tsp45I | GTSAC | 3 cut(s) | 113, 317, 1064 |
| TspDTI | ATGAA | 4 cut(s) | 243, 864, 1278, 1317 |
| TspRI | CASTG | 8 cut(s) | 166, 394, 439, 565, 886, 1054, 1120, 1190 |
| VneI | GTGCAC | 1 cut(s) | 1181 |
| VpaK11BI | GGWCC | 1 cut(s) | 358 |
| XagI | CCTNNNNNAGG | 3 cut(s) | 724, 1073, 1253 |
| XceI | RCATGY | 1 cut(s) | 185 |
| XhoI | CTCGAG | 2 cut(s) | 209, 1250 |
| ZrmI | AGTACT | 1 cut(s) | 1316 |