Rh6CG206500

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6C
OG0000549
Physical Location & Seq
Forward (+)
34371064 .. 34371540
477 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6CG206500.1

Sequence Viewer

Length: 477 bp
ATGCTGAGCCACGACGCCTTCAGCACCATCTTCTCCGAGATCGGTGCCAGCAAGCATGTCCCAGGCGTAATCTTCGCCGAACTCGAGCCCACCATCTTCTTCTCCAAGATCGGTGCAGGCAAGCATATCCCAGGCGTAATCTTCGCCGACCTTGAGCCCACCATCTTATTCTCCGAGATCGGTGCCGGCAAGCATGTGCCAGGCGAAATCTTCGCCGACCTCGAGCCCACCGTCAAAGCCCATCGTCCCAGTCGCTGCTGCTCGTGCTGGAAGCTCCCGGAGGCGCTGGTCCCGATGTGGGAATTCGAGTCAGATAGGAACGTCGGCGTCCACCCCCGACTTGTAAGAATTCTTCCTCACCTCAGTTCTTGTGCTAGGCCGCAGAGACATTGCACCTCTCTTTGCATATCCCAGGTGCTGCAGGTCCTCGTTCGTCTGGGCCGAGCGACGGCGCTGCTGGTGTCGCCGGAGGAGTGA

Protein Analysis

158

Amino Acids

17.35

Weight (kDa)

6.7

Isoelectric Point (pI)

50.23

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000549)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G04820 AT1G50010 AT4G14960 AT4G14960
fragaria_vesca FvH4_1g18660 FvH4_2g03140 FvH4_5g33560 FvH4_6g48940 FvH4_6g48940
malus_domestica MD02G1204800.v1.1 MD05G1106800.v1.1 MD08G1210600.v1.1 MD09G1049500.v1.1 MD10G1111600.v1.1 MD15G1398700.v1.1 MD17G1049700.v1.1
prunus_persica Prupe.1G542600_v2.0.a1 Prupe.3G269300_v2.0.a1 Prupe.6G212700_v2.0.a1 Prupe.8G150900_v2.0.a1
pyrus_communis pycom05g10370 pycom08g18150 pycom10g09640 pycom10g09660 pycom111g04050 pycom15g35470 pycom17g04460
rosa_chinensis RchiOBHm_Chr2g0109481 RchiOBHm_Chr2g0168751 RchiOBHm_Chr3g0453071 RchiOBHm_Chr3g0460741 RchiOBHm_Chr3g0467971 RchiOBHm_Chr4g0400901 RchiOBHm_Chr6g0248011 RchiOBHm_Chr7g0232381 RchiOBHm_Chr7g0238121
rosa_laevigata RLG00000001350 RLG00000009167 RLG00000015163 RLG00000017708 RLG00000017741 RLG00000021807 RLG00000023849 RLG00000026518
rosa_multiflora Rmu_sc0000332.1_g000021 Rmu_sc0000332.1_g000029 Rmu_sc0002416.1_g000007 Rmu_sc0002611.1_g000001 Rmu_sc0003113.1_g000016 Rmu_sc0004780.1_g000001 Rmu_sc0004805.1_g000035 Rmu_sc0010633.1_g000010 Rmu_sc0011069.1_g000003 Rmu_sc0016170.1_g000002 Rmu_sc0025757.1_g000003 Rmu_ssc0000484.1_g000003
rosa_roxburghii Rroxscaffold_2G00134200 Rroxscaffold_4G00321030 Rroxscaffold_7G00213500
rosa_rugosa Rorug01G0283100 Rorug02G0160500 Rorug02G0535300 Rorug04G0027000 Rorug04G0328100 Rorug05G0388700 Rorug05G0530200 Rorug06G0489200 Rorug07G0104900 Rorug07G0209400 Rorug07G0274500
rosa_samantha Rh2BG222800 Rh2BG618900 Rh2CG214400 Rh2CG587200 Rh6BG040200 Rh6CG206500 Rh6DG035600 Rh7BG401600 Rh7BG401700 Rh7DG418400
rosa_wichuraiana Rw2G050240 Rw6G003920 Rw7G035430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 412
AccB1I GGYRCC 2 cut(s) 44, 182
AciI CCGC 1 cut(s) 380
AcsI RAATTY 2 cut(s) 302, 348
AcuI CTGAAG 1 cut(s) 4
AcyI GRCGYC 2 cut(s) 15, 327
AfiI CCNNNNNNNGG 2 cut(s) 298, 448
AjnI CCWGG 4 cut(s) 61, 130, 199, 411
AluBI AGCT 1 cut(s) 274
AluI AGCT 1 cut(s) 274
Alw26I GTCTC 1 cut(s) 379
AlwNI CAGNNNCTG 2 cut(s) 255, 418
Ama87I CYCGRG 2 cut(s) 83, 221
AoxI GGCC 2 cut(s) 377, 439
ApeKI GCWGC 4 cut(s) 255, 258, 418, 454
ApoI RAATTY 2 cut(s) 302, 348
AspLEI GCGC 2 cut(s) 286, 454
AspS9I GGNCC 3 cut(s) 289, 424, 439
AsuC2I CCSGG 1 cut(s) 278
AsuHPI GGTGA 1 cut(s) 350
AvaI CYCGRG 2 cut(s) 83, 221
AvaII GGWCC 2 cut(s) 289, 424
BanI GGYRCC 2 cut(s) 44, 182
BanII GRGCYC 3 cut(s) 90, 159, 228
BarI GAAGNNNNNNTAC 2 cut(s) 336, 368
BauI CACGAG 1 cut(s) 262
BbvI GCAGC 4 cut(s) 242, 245, 405, 441
BccI CCATC 4 cut(s) 35, 101, 170, 249
BceAI ACGGC 1 cut(s) 465
BcgI CGANNNNNNTGC 6 cut(s) 26, 60, 164, 198, 436, 470
BciT130I CCWGG 4 cut(s) 63, 132, 201, 413
BcnI CCSGG 1 cut(s) 278
BcoDI GTCTC 1 cut(s) 379
BfaI CTAG 1 cut(s) 375
BfmI CTRYAG 1 cut(s) 419
BfoI RGCGCY 2 cut(s) 287, 455
BfuAI ACCTGC 1 cut(s) 412
BisI GCNGC 5 cut(s) 256, 259, 380, 419, 455
BlpI GCTNAGC 1 cut(s) 5
BlsI GCNGC 5 cut(s) 257, 260, 381, 420, 456
Bme1390I CCNGG 5 cut(s) 63, 132, 201, 278, 413
Bme18I GGWCC 2 cut(s) 289, 424
BmeT110I CYCGRG 2 cut(s) 83, 221
BmgT120I GGNCC 3 cut(s) 289, 424, 439
BmiI GGNNCC 3 cut(s) 46, 184, 291
BmrFI CCNGG 5 cut(s) 63, 132, 201, 278, 413
BmrI ACTGGG 1 cut(s) 243
BmuI ACTGGG 1 cut(s) 243
Bpu1102I GCTNAGC 1 cut(s) 5
BpuEI CTTGAG 1 cut(s) 173
BpuMI CCSGG 1 cut(s) 278
BsaHI GRCGYC 2 cut(s) 15, 327
BsaJI CCNNGG 3 cut(s) 61, 130, 411
Bsc4I CCNNNNNNNGG 2 cut(s) 298, 448
Bse118I RCCGGY 1 cut(s) 185
Bse1I ACTGG 1 cut(s) 249
Bse3DI GCAATG 1 cut(s) 388
BseBI CCWGG 4 cut(s) 63, 132, 201, 413
BseDI CCNNGG 3 cut(s) 61, 130, 411
BseLI CCNNNNNNNGG 2 cut(s) 298, 448
BseMI GCAATG 1 cut(s) 388
BseMII CTCAG 1 cut(s) 376
BseNI ACTGG 1 cut(s) 249
BseXI GCAGC 4 cut(s) 242, 245, 405, 441
BsgI GTGCAG 1 cut(s) 135
BshFI GGCC 2 cut(s) 379, 441
BshNI GGYRCC 2 cut(s) 44, 182
BsiHKCI CYCGRG 2 cut(s) 83, 221
BsiSI CCGG 3 cut(s) 186, 278, 467
BslFI GGGAC 3 cut(s) 44, 231, 275
BslI CCNNNNNNNGG 2 cut(s) 298, 448
BsmAI GTCTC 1 cut(s) 379
BsmFI GGGAC 3 cut(s) 44, 231, 275
BsnI GGCC 2 cut(s) 379, 441
BsoBI CYCGRG 2 cut(s) 83, 221
Bsp1286I GDGCHC 3 cut(s) 90, 159, 228
Bsp143I GATC 3 cut(s) 39, 108, 177
Bsp1720I GCTNAGC 1 cut(s) 5
BspACI CCGC 1 cut(s) 380
BspANI GGCC 2 cut(s) 379, 441
BspCNI CTCAG 1 cut(s) 375
BspLI GGNNCC 3 cut(s) 46, 184, 291
BspMAI CTGCAG 1 cut(s) 423
BspMI ACCTGC 1 cut(s) 412
BspT107I GGYRCC 2 cut(s) 44, 182
BsrDI GCAATG 1 cut(s) 388
BsrFI RCCGGY 1 cut(s) 185
BsrI ACTGG 1 cut(s) 249
BssAI RCCGGY 1 cut(s) 185
BssECI CCNNGG 3 cut(s) 61, 130, 411
BssMI GATC 3 cut(s) 39, 108, 177
BssNI GRCGYC 2 cut(s) 15, 327
BssSI CACGAG 1 cut(s) 262
Bst2BI CACGAG 1 cut(s) 262
Bst2UI CCWGG 4 cut(s) 63, 132, 201, 413
Bst4CI ACNGT 1 cut(s) 232
BstACI GRCGYC 2 cut(s) 15, 327
BstC8I GCNNGC 6 cut(s) 49, 53, 118, 122, 187, 191
BstDEI CTNAG 2 cut(s) 5, 362
BstH2I RGCGCY 2 cut(s) 287, 455
BstHHI GCGC 2 cut(s) 286, 454
BstKTI GATC 3 cut(s) 42, 111, 180
BstMAI GTCTC 1 cut(s) 379
BstMBI GATC 3 cut(s) 39, 108, 177
BstMWI GCNNNNNNNGC 2 cut(s) 264, 463
BstNI CCWGG 4 cut(s) 63, 132, 201, 413
BstNSI RCATGY 2 cut(s) 59, 197
BstSCI CCNGG 5 cut(s) 61, 130, 199, 276, 411
BstSFI CTRYAG 1 cut(s) 419
BstV1I GCAGC 4 cut(s) 242, 245, 405, 441
BsuRI GGCC 2 cut(s) 379, 441
BveI ACCTGC 1 cut(s) 412
Cac8I GCNNGC 6 cut(s) 49, 53, 118, 122, 187, 191
CaiI CAGNNNCTG 2 cut(s) 255, 418
CfoI GCGC 2 cut(s) 286, 454
Cfr10I RCCGGY 1 cut(s) 185
Cfr13I GGNCC 3 cut(s) 289, 424, 439
CseI GACGC 2 cut(s) 23, 316
CviAII CATG 2 cut(s) 56, 194
CviJI RGCY 8 cut(s) 9, 88, 157, 226, 239, 274, 379, 441
CviKI_1 RGCY 8 cut(s) 9, 88, 157, 226, 239, 274, 379, 441
DdeI CTNAG 2 cut(s) 5, 362
DpnI GATC 3 cut(s) 41, 110, 179
DpnII GATC 3 cut(s) 39, 108, 177
Eco24I GRGCYC 3 cut(s) 90, 159, 228
Eco47I GGWCC 2 cut(s) 289, 424
Eco57I CTGAAG 1 cut(s) 4
Eco88I CYCGRG 2 cut(s) 83, 221
EcoO109I RGGNCCY 1 cut(s) 424
EcoRI GAATTC 2 cut(s) 302, 348
EcoRII CCWGG 4 cut(s) 61, 130, 199, 411
EcoT38I GRGCYC 3 cut(s) 90, 159, 228
FaeI CATG 2 cut(s) 59, 197
FaiI YATR 4 cut(s) 57, 126, 195, 407
FaqI GGGAC 3 cut(s) 44, 231, 275
FatI CATG 2 cut(s) 55, 193
Fnu4HI GCNGC 5 cut(s) 256, 259, 380, 419, 455
FriOI GRGCYC 3 cut(s) 90, 159, 228
Fsp4HI GCNGC 5 cut(s) 256, 259, 380, 419, 455
FspBI CTAG 1 cut(s) 375
GlaI GCGC 2 cut(s) 285, 453
GluI GCNGC 5 cut(s) 256, 259, 380, 419, 455
HaeII RGCGCY 2 cut(s) 287, 455
HaeIII GGCC 2 cut(s) 379, 441
HapII CCGG 3 cut(s) 186, 278, 467
HgaI GACGC 2 cut(s) 23, 316
HhaI GCGC 2 cut(s) 286, 454
Hin1I GRCGYC 2 cut(s) 15, 327
Hin1II CATG 2 cut(s) 59, 197
Hin6I GCGC 2 cut(s) 284, 452
HinP1I GCGC 2 cut(s) 284, 452
HinfI GANTC 1 cut(s) 308
HpaII CCGG 3 cut(s) 186, 278, 467
HphI GGTGA 1 cut(s) 350
Hpy166II GTNNAC 1 cut(s) 331
Hpy188I TCNGA 3 cut(s) 37, 175, 313
Hpy188III TCNNGA 1 cut(s) 292
Hpy8I GTNNAC 1 cut(s) 331
Hpy99I CGWCG 3 cut(s) 17, 326, 451
HpyAV CCTTC 1 cut(s) 28
HpyCH4III ACNGT 1 cut(s) 232
HpyCH4IV ACGT 1 cut(s) 321
HpyCH4V TGCA 4 cut(s) 116, 393, 405, 421
HpyF10VI GCNNNNNNNGC 2 cut(s) 264, 463
HpyF3I CTNAG 2 cut(s) 5, 362
HpySE526I ACGT 1 cut(s) 321
Hsp92I GRCGYC 2 cut(s) 15, 327
Hsp92II CATG 2 cut(s) 59, 197
HspAI GCGC 2 cut(s) 284, 452
KroI GCCGGC 1 cut(s) 185
KroNI GCCGGC 1 cut(s) 187
Kzo9I GATC 3 cut(s) 39, 108, 177
LmnI GCTCC 1 cut(s) 279
Lsp1109I GCAGC 4 cut(s) 242, 245, 405, 441
MaeI CTAG 1 cut(s) 375
MaeII ACGT 1 cut(s) 321
MalI GATC 3 cut(s) 41, 110, 179
MboI GATC 3 cut(s) 39, 108, 177
MboII GAAGA 7 cut(s) 22, 64, 88, 91, 133, 202, 344
MhlI GDGCHC 3 cut(s) 90, 159, 228
MluCI AATT 2 cut(s) 302, 348
MlyI GAGTC 1 cut(s) 317
MnlI CCTC 7 cut(s) 230, 274, 366, 371, 406, 437, 463
MroNI GCCGGC 1 cut(s) 185
MspI CCGG 3 cut(s) 186, 278, 467
MspR9I CCNGG 5 cut(s) 63, 132, 201, 278, 413
MvaI CCWGG 4 cut(s) 63, 132, 201, 413
MwoI GCNNNNNNNGC 2 cut(s) 264, 463
NaeI GCCGGC 1 cut(s) 187
NciI CCSGG 1 cut(s) 278
NdeII GATC 3 cut(s) 39, 108, 177
NgoMIV GCCGGC 1 cut(s) 185
NlaIII CATG 2 cut(s) 59, 197
NlaIV GGNNCC 3 cut(s) 46, 184, 291
NmeAIII GCCGAG 1 cut(s) 467
NspI RCATGY 2 cut(s) 59, 197
PaeR7I CTCGAG 2 cut(s) 83, 221
PcsI WCGNNNNNNNCGW 4 cut(s) 81, 219, 228, 439
PdiI GCCGGC 1 cut(s) 187
PfoI TCCNGGA 1 cut(s) 276
PkrI GCNGC 5 cut(s) 257, 260, 381, 420, 456
PleI GAGTC 1 cut(s) 316
PpsI GAGTC 1 cut(s) 316
PpuMI RGGWCCY 1 cut(s) 424
Psp5II RGGWCCY 1 cut(s) 424
Psp6I CCWGG 4 cut(s) 61, 130, 199, 411
PspGI CCWGG 4 cut(s) 61, 130, 199, 411
PspN4I GGNNCC 3 cut(s) 46, 184, 291
PspPI GGNCC 3 cut(s) 289, 424, 439
PspPPI RGGWCCY 1 cut(s) 424
PspXI VCTCGAGB 2 cut(s) 83, 221
PstI CTGCAG 1 cut(s) 423
PstNI CAGNNNCTG 2 cut(s) 255, 418
SatI GCNGC 5 cut(s) 256, 259, 380, 419, 455
Sau3AI GATC 3 cut(s) 39, 108, 177
Sau96I GGNCC 3 cut(s) 289, 424, 439
SchI GAGTC 1 cut(s) 317
ScrFI CCNGG 5 cut(s) 63, 132, 201, 278, 413
SduI GDGCHC 3 cut(s) 90, 159, 228
SetI ASST 8 cut(s) 153, 222, 276, 324, 363, 398, 417, 426
SfcI CTRYAG 1 cut(s) 419
Sfr274I CTCGAG 2 cut(s) 83, 221
SinI GGWCC 2 cut(s) 289, 424
SlaI CTCGAG 2 cut(s) 83, 221
SmlI CTYRAG 3 cut(s) 83, 152, 221
SmoI CTYRAG 3 cut(s) 83, 152, 221
Sse9I AATT 2 cut(s) 302, 348
SsiI CCGC 1 cut(s) 380
SspMI CTAG 1 cut(s) 375
StyD4I CCNGG 5 cut(s) 61, 130, 199, 276, 411
TaaI ACNGT 1 cut(s) 232
TaiI ACGT 1 cut(s) 324
TaqI TCGA 3 cut(s) 84, 222, 306
TasI AATT 2 cut(s) 302, 348
TauI GCSGC 1 cut(s) 382
TseI GCWGC 4 cut(s) 255, 258, 418, 454
VpaK11BI GGWCC 2 cut(s) 289, 424
XapI RAATTY 2 cut(s) 302, 348
XceI RCATGY 2 cut(s) 59, 197
XhoI CTCGAG 2 cut(s) 83, 221
XspI CTAG 1 cut(s) 375
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.