Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 1 cut(s) | 122 |
| Acc36I | ACCTGC | 1 cut(s) | 633 |
| AccB1I | GGYRCC | 1 cut(s) | 1038 |
| AccI | GTMKAC | 1 cut(s) | 513 |
| AccII | CGCG | 1 cut(s) | 1199 |
| AclWI | GGATC | 1 cut(s) | 457 |
| AcoI | YGGCCR | 5 cut(s) | 28, 100, 990, 996, 1104 |
| AcuI | CTGAAG | 2 cut(s) | 142, 1341 |
| AcyI | GRCGYC | 2 cut(s) | 294, 896 |
| AfaI | GTAC | 3 cut(s) | 388, 956, 1196 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 926, 1108 |
| AgsI | TTSAA | 5 cut(s) | 148, 398, 548, 659, 1054 |
| AjiI | CACGTC | 2 cut(s) | 184, 754 |
| AjnI | CCWGG | 3 cut(s) | 30, 1090, 1101 |
| AleI | CACNNNNGTG | 1 cut(s) | 596 |
| AloI | GAACNNNNNNTCC | 4 cut(s) | 72, 104, 954, 986 |
| AluBI | AGCT | 4 cut(s) | 67, 256, 274, 373 |
| AluI | AGCT | 4 cut(s) | 67, 256, 274, 373 |
| Alw21I | GWGCWC | 2 cut(s) | 591, 1218 |
| Alw26I | GTCTC | 4 cut(s) | 129, 525, 535, 651 |
| Alw44I | GTGCAC | 1 cut(s) | 1214 |
| AlwI | GGATC | 1 cut(s) | 457 |
| Ama87I | CYCGRG | 3 cut(s) | 500, 1263, 1283 |
| ApaLI | GTGCAC | 1 cut(s) | 1214 |
| ApeKI | GCWGC | 1 cut(s) | 271 |
| AspLEI | GCGC | 3 cut(s) | 740, 884, 1282 |
| AspS9I | GGNCC | 4 cut(s) | 205, 237, 778, 1017 |
| AsuC2I | CCSGG | 2 cut(s) | 266, 391 |
| AsuHPI | GGTGA | 4 cut(s) | 865, 1241, 1337, 1346 |
| AvaI | CYCGRG | 3 cut(s) | 500, 1263, 1283 |
| AvaII | GGWCC | 4 cut(s) | 205, 237, 778, 1017 |
| AxyI | CCTNAGG | 1 cut(s) | 525 |
| BaeGI | GKGCMC | 2 cut(s) | 1043, 1218 |
| BalI | TGGCCA | 3 cut(s) | 102, 998, 1106 |
| BanI | GGYRCC | 1 cut(s) | 1038 |
| BauI | CACGAG | 1 cut(s) | 848 |
| BbsI | GAAGAC | 2 cut(s) | 297, 1344 |
| Bbv12I | GWGCWC | 2 cut(s) | 591, 1218 |
| BbvCI | CCTCAGC | 1 cut(s) | 1155 |
| BbvI | GCAGC | 1 cut(s) | 283 |
| BccI | CCATC | 5 cut(s) | 26, 92, 632, 898, 1325 |
| BceAI | ACGGC | 5 cut(s) | 100, 179, 404, 751, 977 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 631, 665 |
| BciT130I | CCWGG | 3 cut(s) | 32, 1092, 1103 |
| BclI | TGATCA | 1 cut(s) | 1174 |
| BcnI | CCSGG | 2 cut(s) | 266, 391 |
| BcoDI | GTCTC | 4 cut(s) | 129, 525, 535, 651 |
| BfoI | RGCGCY | 2 cut(s) | 885, 1283 |
| BfuAI | ACCTGC | 1 cut(s) | 633 |
| BisI | GCNGC | 8 cut(s) | 272, 283, 297, 640, 990, 1060, 1096, 1278 |
| BlsI | GCNGC | 8 cut(s) | 273, 284, 298, 641, 991, 1061, 1097, 1279 |
| Bme1390I | CCNGG | 5 cut(s) | 32, 266, 391, 1092, 1103 |
| Bme18I | GGWCC | 4 cut(s) | 205, 237, 778, 1017 |
| BmeT110I | CYCGRG | 3 cut(s) | 500, 1263, 1283 |
| BmgBI | CACGTC | 2 cut(s) | 184, 754 |
| BmgT120I | GGNCC | 4 cut(s) | 205, 237, 778, 1017 |
| BmiI | GGNNCC | 5 cut(s) | 172, 433, 780, 972, 1040 |
| BmrFI | CCNGG | 5 cut(s) | 32, 266, 391, 1092, 1103 |
| BmrI | ACTGGG | 1 cut(s) | 1056 |
| BmsI | GCATC | 4 cut(s) | 21, 96, 96, 1071 |
| BmuI | ACTGGG | 1 cut(s) | 1056 |
| BpiI | GAAGAC | 2 cut(s) | 297, 1344 |
| BplI | GAGNNNNNCTC | 3 cut(s) | 31, 842, 874 |
| Bpu10I | CCTNAGC | 1 cut(s) | 1155 |
| BpuEI | CTTGAG | 2 cut(s) | 230, 1331 |
| BpuMI | CCSGG | 2 cut(s) | 266, 391 |
| Bsa29I | ATCGAT | 1 cut(s) | 225 |
| BsaHI | GRCGYC | 2 cut(s) | 294, 896 |
| BsaI | GGTCTC | 2 cut(s) | 535, 651 |
| BsaJI | CCNNGG | 4 cut(s) | 445, 920, 993, 1107 |
| BsaWI | WCCGGW | 1 cut(s) | 167 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 954, 984 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 926, 1108 |
| Bse118I | RCCGGY | 3 cut(s) | 239, 434, 892 |
| Bse1I | ACTGG | 3 cut(s) | 1040, 1051, 1223 |
| Bse21I | CCTNAGG | 1 cut(s) | 525 |
| BseBI | CCWGG | 3 cut(s) | 32, 1092, 1103 |
| BseCI | ATCGAT | 1 cut(s) | 225 |
| BseDI | CCNNGG | 4 cut(s) | 445, 920, 993, 1107 |
| BseGI | GGATG | 2 cut(s) | 18, 87 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 926, 1108 |
| BseMII | CTCAG | 3 cut(s) | 539, 710, 1146 |
| BseNI | ACTGG | 3 cut(s) | 1040, 1051, 1223 |
| BseRI | GAGGAG | 2 cut(s) | 513, 599 |
| BseSI | GKGCMC | 2 cut(s) | 1043, 1218 |
| BseX3I | CGGCCG | 1 cut(s) | 990 |
| BseXI | GCAGC | 1 cut(s) | 283 |
| BseYI | CCCAGC | 1 cut(s) | 60 |
| Bsh1236I | CGCG | 1 cut(s) | 1199 |
| Bsh1285I | CGRYCG | 1 cut(s) | 993 |
| BshNI | GGYRCC | 1 cut(s) | 1038 |
| BshVI | ATCGAT | 1 cut(s) | 225 |
| BsiEI | CGRYCG | 1 cut(s) | 993 |
| BsiHKAI | GWGCWC | 2 cut(s) | 591, 1218 |
| BsiHKCI | CYCGRG | 3 cut(s) | 500, 1263, 1283 |
| BsiSI | CCGG | 8 cut(s) | 36, 168, 174, 240, 266, 390, 435, 893 |
| BslFI | GGGAC | 2 cut(s) | 170, 764 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 926, 1108 |
| BsmAI | GTCTC | 4 cut(s) | 129, 525, 535, 651 |
| BsmBI | CGTCTC | 2 cut(s) | 129, 525 |
| BsmFI | GGGAC | 2 cut(s) | 170, 764 |
| Bso31I | GGTCTC | 2 cut(s) | 535, 651 |
| BsoBI | CYCGRG | 3 cut(s) | 500, 1263, 1283 |
| Bsp1286I | GDGCHC | 3 cut(s) | 591, 1043, 1218 |
| Bsp1407I | TGTACA | 1 cut(s) | 954 |
| Bsp143I | GATC | 7 cut(s) | 346, 358, 449, 825, 870, 1174, 1186 |
| BspCNI | CTCAG | 3 cut(s) | 538, 709, 1147 |
| BspDI | ATCGAT | 1 cut(s) | 225 |
| BspFNI | CGCG | 1 cut(s) | 1199 |
| BspLI | GGNNCC | 5 cut(s) | 172, 433, 780, 972, 1040 |
| BspMI | ACCTGC | 1 cut(s) | 633 |
| BspPI | GGATC | 1 cut(s) | 457 |
| BspT107I | GGYRCC | 1 cut(s) | 1038 |
| BspTNI | GGTCTC | 2 cut(s) | 535, 651 |
| BsrFI | RCCGGY | 3 cut(s) | 239, 434, 892 |
| BsrGI | TGTACA | 1 cut(s) | 954 |
| BsrI | ACTGG | 3 cut(s) | 1040, 1051, 1223 |
| BssAI | RCCGGY | 3 cut(s) | 239, 434, 892 |
| BssECI | CCNNGG | 4 cut(s) | 445, 920, 993, 1107 |
| BssMI | GATC | 7 cut(s) | 346, 358, 449, 825, 870, 1174, 1186 |
| BssNI | GRCGYC | 2 cut(s) | 294, 896 |
| BssSI | CACGAG | 1 cut(s) | 848 |
| BssT1I | CCWWGG | 2 cut(s) | 920, 1107 |
| Bst2BI | CACGAG | 1 cut(s) | 848 |
| Bst2UI | CCWGG | 3 cut(s) | 32, 1092, 1103 |
| Bst4CI | ACNGT | 5 cut(s) | 124, 220, 386, 511, 1084 |
| BstACI | GRCGYC | 2 cut(s) | 294, 896 |
| BstAUI | TGTACA | 1 cut(s) | 954 |
| BstC8I | GCNNGC | 7 cut(s) | 58, 254, 375, 563, 894, 943, 1206 |
| BstDEI | CTNAG | 3 cut(s) | 525, 696, 1155 |
| BstDSI | CCRYGG | 1 cut(s) | 993 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 1106 |
| BstF5I | GGATG | 2 cut(s) | 18, 87 |
| BstFNI | CGCG | 1 cut(s) | 1199 |
| BstH2I | RGCGCY | 2 cut(s) | 885, 1283 |
| BstHHI | GCGC | 3 cut(s) | 740, 884, 1282 |
| BstKTI | GATC | 7 cut(s) | 349, 361, 452, 828, 873, 1177, 1189 |
| BstMAI | GTCTC | 4 cut(s) | 129, 525, 535, 651 |
| BstMBI | GATC | 7 cut(s) | 346, 358, 449, 825, 870, 1174, 1186 |
| BstMCI | CGRYCG | 1 cut(s) | 993 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 249, 839, 995, 1205 |
| BstNI | CCWGG | 3 cut(s) | 32, 1092, 1103 |
| BstSCI | CCNGG | 5 cut(s) | 30, 264, 389, 1090, 1101 |
| BstSLI | GKGCMC | 2 cut(s) | 1043, 1218 |
| BstUI | CGCG | 1 cut(s) | 1199 |
| BstV1I | GCAGC | 1 cut(s) | 283 |
| BstV2I | GAAGAC | 2 cut(s) | 297, 1344 |
| BstX2I | RGATCY | 1 cut(s) | 449 |
| BstXI | CCANNNNNNTGG | 2 cut(s) | 98, 422 |
| BstYI | RGATCY | 1 cut(s) | 449 |
| BstZI | CGGCCG | 1 cut(s) | 990 |
| Bsu15I | ATCGAT | 1 cut(s) | 225 |
| Bsu36I | CCTNAGG | 1 cut(s) | 525 |
| BsuTUI | ATCGAT | 1 cut(s) | 225 |
| BtgI | CCRYGG | 1 cut(s) | 993 |
| BtrI | CACGTC | 2 cut(s) | 184, 754 |
| BtsCI | GGATG | 2 cut(s) | 18, 87 |
| BtsI | GCAGTG | 1 cut(s) | 714 |
| BtsIMutI | CAGTG | 2 cut(s) | 714, 1216 |
| BveI | ACCTGC | 1 cut(s) | 633 |
| Cac8I | GCNNGC | 7 cut(s) | 58, 254, 375, 563, 894, 943, 1206 |
| CfoI | GCGC | 3 cut(s) | 740, 884, 1282 |
| Cfr10I | RCCGGY | 3 cut(s) | 239, 434, 892 |
| Cfr13I | GGNCC | 4 cut(s) | 205, 237, 778, 1017 |
| ClaI | ATCGAT | 1 cut(s) | 225 |
| CseI | GACGC | 3 cut(s) | 149, 302, 885 |
| Csp6I | GTAC | 3 cut(s) | 387, 955, 1195 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 1132, 1167 |
| CviAII | CATG | 4 cut(s) | 802, 937, 1129, 1237 |
| CviQI | GTAC | 3 cut(s) | 387, 955, 1195 |
| DdeI | CTNAG | 3 cut(s) | 525, 696, 1155 |
| DpnI | GATC | 7 cut(s) | 348, 360, 451, 827, 872, 1176, 1188 |
| DpnII | GATC | 7 cut(s) | 346, 358, 449, 825, 870, 1174, 1186 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 122 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 122 |
| EaeI | YGGCCR | 5 cut(s) | 28, 100, 990, 996, 1104 |
| EagI | CGGCCG | 1 cut(s) | 990 |
| EciI | GGCGGA | 1 cut(s) | 147 |
| EclXI | CGGCCG | 1 cut(s) | 990 |
| Eco130I | CCWWGG | 2 cut(s) | 920, 1107 |
| Eco147I | AGGCCT | 1 cut(s) | 842 |
| Eco31I | GGTCTC | 2 cut(s) | 535, 651 |
| Eco47I | GGWCC | 4 cut(s) | 205, 237, 778, 1017 |
| Eco52I | CGGCCG | 1 cut(s) | 990 |
| Eco57I | CTGAAG | 2 cut(s) | 142, 1341 |
| Eco81I | CCTNAGG | 1 cut(s) | 525 |
| Eco88I | CYCGRG | 3 cut(s) | 500, 1263, 1283 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 1106 |
| EcoRII | CCWGG | 3 cut(s) | 30, 1090, 1101 |
| EcoT14I | CCWWGG | 2 cut(s) | 920, 1107 |
| ErhI | CCWWGG | 2 cut(s) | 920, 1107 |
| Esp3I | CGTCTC | 2 cut(s) | 129, 525 |
| FaeI | CATG | 4 cut(s) | 805, 940, 1132, 1240 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 652, 684, 1293, 1325 |
| FaqI | GGGAC | 2 cut(s) | 170, 764 |
| FatI | CATG | 4 cut(s) | 801, 936, 1128, 1236 |
| FbaI | TGATCA | 1 cut(s) | 1174 |
| FblI | GTMKAC | 1 cut(s) | 513 |
| Fnu4HI | GCNGC | 8 cut(s) | 272, 283, 297, 640, 990, 1060, 1096, 1278 |
| FokI | GGATG | 2 cut(s) | 5, 74 |
| Fsp4HI | GCNGC | 8 cut(s) | 272, 283, 297, 640, 990, 1060, 1096, 1278 |
| GlaI | GCGC | 3 cut(s) | 739, 883, 1281 |
| GluI | GCNGC | 8 cut(s) | 272, 283, 297, 640, 990, 1060, 1096, 1278 |
| GsaI | CCCAGC | 1 cut(s) | 64 |
| HaeII | RGCGCY | 2 cut(s) | 885, 1283 |
| HapII | CCGG | 8 cut(s) | 36, 168, 174, 240, 266, 390, 435, 893 |
| HgaI | GACGC | 3 cut(s) | 149, 302, 885 |
| HhaI | GCGC | 3 cut(s) | 740, 884, 1282 |
| Hin1I | GRCGYC | 2 cut(s) | 294, 896 |
| Hin1II | CATG | 4 cut(s) | 805, 940, 1132, 1240 |
| Hin6I | GCGC | 3 cut(s) | 738, 882, 1280 |
| HinP1I | GCGC | 3 cut(s) | 738, 882, 1280 |
| HindIII | AAGCTT | 1 cut(s) | 371 |
| HinfI | GANTC | 4 cut(s) | 39, 363, 792, 1313 |
| HpaII | CCGG | 8 cut(s) | 36, 168, 174, 240, 266, 390, 435, 893 |
| HphI | GGTGA | 4 cut(s) | 865, 1241, 1337, 1346 |
| Hpy166II | GTNNAC | 5 cut(s) | 507, 514, 751, 1033, 1216 |
| Hpy188I | TCNGA | 7 cut(s) | 50, 368, 449, 975, 1116, 1191, 1258 |
| Hpy188III | TCNNGA | 6 cut(s) | 235, 650, 766, 1006, 1265, 1285 |
| Hpy8I | GTNNAC | 5 cut(s) | 507, 514, 751, 1033, 1216 |
| Hpy99I | CGWCG | 4 cut(s) | 128, 143, 545, 737 |
| HpyAV | CCTTC | 5 cut(s) | 163, 328, 1238, 1282, 1316 |
| HpyCH4III | ACNGT | 5 cut(s) | 124, 220, 386, 511, 1084 |
| HpyCH4IV | ACGT | 2 cut(s) | 183, 753 |
| HpyCH4V | TGCA | 4 cut(s) | 12, 866, 1128, 1216 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 249, 839, 995, 1205 |
| HpyF3I | CTNAG | 3 cut(s) | 525, 696, 1155 |
| HpySE526I | ACGT | 2 cut(s) | 183, 753 |
| Hsp92I | GRCGYC | 2 cut(s) | 294, 896 |
| Hsp92II | CATG | 4 cut(s) | 805, 940, 1132, 1240 |
| HspAI | GCGC | 3 cut(s) | 738, 882, 1280 |
| KroI | GCCGGC | 1 cut(s) | 892 |
| KroNI | GCCGGC | 1 cut(s) | 894 |
| Ksp22I | TGATCA | 1 cut(s) | 1174 |
| Kzo9I | GATC | 7 cut(s) | 346, 358, 449, 825, 870, 1174, 1186 |
| LmnI | GCTCC | 4 cut(s) | 64, 170, 268, 586 |
| Lsp1109I | GCAGC | 1 cut(s) | 283 |
| LweI | GCATC | 4 cut(s) | 21, 96, 96, 1071 |
| MaeII | ACGT | 2 cut(s) | 183, 753 |
| MaeIII | GTNAC | 3 cut(s) | 113, 754, 1325 |
| MalI | GATC | 7 cut(s) | 348, 360, 451, 827, 872, 1176, 1188 |
| MboI | GATC | 7 cut(s) | 346, 358, 449, 825, 870, 1174, 1186 |
| MboII | GAAGA | 3 cut(s) | 148, 302, 1349 |
| MfeI | CAATTG | 1 cut(s) | 327 |
| MflI | RGATCY | 1 cut(s) | 449 |
| MhlI | GDGCHC | 3 cut(s) | 591, 1043, 1218 |
| MlsI | TGGCCA | 3 cut(s) | 102, 998, 1106 |
| MluCI | AATT | 2 cut(s) | 327, 772 |
| MluNI | TGGCCA | 3 cut(s) | 102, 998, 1106 |
| MlyI | GAGTC | 1 cut(s) | 1322 |
| MmeI | TCCRAC | 3 cut(s) | 28, 403, 1161 |
| Mox20I | TGGCCA | 3 cut(s) | 102, 998, 1106 |
| MroNI | GCCGGC | 1 cut(s) | 892 |
| MscI | TGGCCA | 3 cut(s) | 102, 998, 1106 |
| MslI | CAYNNNNRTG | 3 cut(s) | 594, 596, 1221 |
| Msp20I | TGGCCA | 3 cut(s) | 102, 998, 1106 |
| MspA1I | CMGCKG | 2 cut(s) | 256, 282 |
| MspI | CCGG | 8 cut(s) | 36, 168, 174, 240, 266, 390, 435, 893 |
| MspR9I | CCNGG | 5 cut(s) | 32, 266, 391, 1092, 1103 |
| MunI | CAATTG | 1 cut(s) | 327 |
| MvaI | CCWGG | 3 cut(s) | 32, 1092, 1103 |
| MvnI | CGCG | 1 cut(s) | 1199 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 249, 839, 995, 1205 |
| NaeI | GCCGGC | 1 cut(s) | 894 |
| NciI | CCSGG | 2 cut(s) | 266, 391 |
| NdeII | GATC | 7 cut(s) | 346, 358, 449, 825, 870, 1174, 1186 |
| NgoMIV | GCCGGC | 1 cut(s) | 892 |
| NlaIII | CATG | 4 cut(s) | 805, 940, 1132, 1240 |
| NlaIV | GGNNCC | 5 cut(s) | 172, 433, 780, 972, 1040 |
| NmeAIII | GCCGAG | 1 cut(s) | 135 |
| NmuCI | GTSAC | 3 cut(s) | 113, 754, 1325 |
| OliI | CACNNNNGTG | 1 cut(s) | 596 |
| PaeR7I | CTCGAG | 1 cut(s) | 1283 |
| PceI | AGGCCT | 1 cut(s) | 842 |
| PdiI | GCCGGC | 1 cut(s) | 894 |
| PfeI | GAWTC | 3 cut(s) | 39, 363, 792 |
| PkrI | GCNGC | 8 cut(s) | 273, 284, 298, 641, 991, 1061, 1097, 1279 |
| PleI | GAGTC | 1 cut(s) | 1321 |
| PpsI | GAGTC | 1 cut(s) | 1321 |
| Psp6I | CCWGG | 3 cut(s) | 30, 1090, 1101 |
| PspFI | CCCAGC | 1 cut(s) | 60 |
| PspGI | CCWGG | 3 cut(s) | 30, 1090, 1101 |
| PspN4I | GGNNCC | 5 cut(s) | 172, 433, 780, 972, 1040 |
| PspPI | GGNCC | 4 cut(s) | 205, 237, 778, 1017 |
| PsuI | RGATCY | 1 cut(s) | 449 |
| PvuII | CAGCTG | 1 cut(s) | 256 |
| RsaI | GTAC | 3 cut(s) | 388, 956, 1196 |
| RsaNI | GTAC | 3 cut(s) | 387, 955, 1195 |
| RseI | CAYNNNNRTG | 3 cut(s) | 594, 596, 1221 |
| SatI | GCNGC | 8 cut(s) | 272, 283, 297, 640, 990, 1060, 1096, 1278 |
| Sau3AI | GATC | 7 cut(s) | 346, 358, 449, 825, 870, 1174, 1186 |
| Sau96I | GGNCC | 4 cut(s) | 205, 237, 778, 1017 |
| SchI | GAGTC | 1 cut(s) | 1322 |
| ScrFI | CCNGG | 5 cut(s) | 32, 266, 391, 1092, 1103 |
| SduI | GDGCHC | 3 cut(s) | 591, 1043, 1218 |
| SfaNI | GCATC | 4 cut(s) | 21, 96, 96, 1071 |
| Sfr274I | CTCGAG | 1 cut(s) | 1283 |
| SinI | GGWCC | 4 cut(s) | 205, 237, 778, 1017 |
| SlaI | CTCGAG | 1 cut(s) | 1283 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 594, 596, 1221 |
| SmlI | CTYRAG | 3 cut(s) | 209, 1283, 1310 |
| SmoI | CTYRAG | 3 cut(s) | 209, 1283, 1310 |
| Sse9I | AATT | 2 cut(s) | 327, 772 |
| SseBI | AGGCCT | 1 cut(s) | 842 |
| StuI | AGGCCT | 1 cut(s) | 842 |
| StyD4I | CCNGG | 5 cut(s) | 30, 264, 389, 1090, 1101 |
| StyI | CCWWGG | 2 cut(s) | 920, 1107 |
| TaaI | ACNGT | 5 cut(s) | 124, 220, 386, 511, 1084 |
| TaiI | ACGT | 2 cut(s) | 186, 756 |
| TasI | AATT | 2 cut(s) | 327, 772 |
| TatI | WGTACW | 1 cut(s) | 954 |
| TauI | GCSGC | 7 cut(s) | 285, 299, 642, 992, 1062, 1098, 1280 |
| TfiI | GAWTC | 3 cut(s) | 39, 363, 792 |
| TscAI | CASTG | 2 cut(s) | 721, 1223 |
| TseFI | GTSAC | 3 cut(s) | 113, 754, 1325 |
| TseI | GCWGC | 1 cut(s) | 271 |
| Tsp45I | GTSAC | 3 cut(s) | 113, 754, 1325 |
| TspDTI | ATGAA | 1 cut(s) | 790 |
| TspGWI | ACGGA | 4 cut(s) | 498, 529, 773, 942 |
| TspRI | CASTG | 2 cut(s) | 721, 1223 |
| VneI | GTGCAC | 1 cut(s) | 1214 |
| VpaK11BI | GGWCC | 4 cut(s) | 205, 237, 778, 1017 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 1106 |
| XcmI | CCANNNNNNNNNTGG | 2 cut(s) | 326, 1027 |
| XhoI | CTCGAG | 1 cut(s) | 1283 |
| XmiI | GTMKAC | 1 cut(s) | 513 |