RLG00000023849

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0000549
Physical Location & Seq
Reverse (-)
29136816 .. 29139201
2386 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000023849

Sequence Viewer

Length: 429 bp
ATGCTAAGCGACATGACCGTCGGTCGCGACGACGAAGCCTTCAACACCTTCTTCTCCAAGACCGGCTCCAGCAAGCATGTCCCACGTGCCATCTTTGTCCACCTCGAGCCCACCGTCATTGATGAGGTCCGCACTAGAACCTGCCGCCATCTCTTCCACCCTGAGCAACTCATCTTTAGCAAGGAGGACGCCGCCAACAACTTCTCCGGTAGTCACTACACCATCGACAAAGAGATCGTCGATCTCTGCCAGGATCAGATCCAGAAGCTCGCTGATAACTGCACTAGGCTCCAGGGATTCCTTGTCTTCAATGCCGTCGGTGGCGGTACTGGGTCGGGTCTTGGGTTGCTTCTGCTGGAGAGGCTTTCTGTGGACTATGGGAAAAAGTCCAAGCTTGGATTCACTGTGTATCCTTCTCCTCAGGTTTAA

Protein Analysis

143

Amino Acids

15.78

Weight (kDa)

6.18

Isoelectric Point (pI)

24.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tubulin PF00091 13 - 139 2e-33 Tubulin/FtsZ family, GTPase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000549)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G04820 AT1G50010 AT4G14960 AT4G14960
fragaria_vesca FvH4_1g18660 FvH4_2g03140 FvH4_5g33560 FvH4_6g48940 FvH4_6g48940
malus_domestica MD02G1204800.v1.1 MD05G1106800.v1.1 MD08G1210600.v1.1 MD09G1049500.v1.1 MD10G1111600.v1.1 MD15G1398700.v1.1 MD17G1049700.v1.1
prunus_persica Prupe.1G542600_v2.0.a1 Prupe.3G269300_v2.0.a1 Prupe.6G212700_v2.0.a1 Prupe.8G150900_v2.0.a1
pyrus_communis pycom05g10370 pycom08g18150 pycom10g09640 pycom10g09660 pycom111g04050 pycom15g35470 pycom17g04460
rosa_chinensis RchiOBHm_Chr2g0109481 RchiOBHm_Chr2g0168751 RchiOBHm_Chr3g0453071 RchiOBHm_Chr3g0460741 RchiOBHm_Chr3g0467971 RchiOBHm_Chr4g0400901 RchiOBHm_Chr6g0248011 RchiOBHm_Chr7g0232381 RchiOBHm_Chr7g0238121
rosa_laevigata RLG00000001350 RLG00000009167 RLG00000015163 RLG00000017708 RLG00000017741 RLG00000021807 RLG00000023849 RLG00000026518
rosa_multiflora Rmu_sc0000332.1_g000021 Rmu_sc0000332.1_g000029 Rmu_sc0002416.1_g000007 Rmu_sc0002611.1_g000001 Rmu_sc0003113.1_g000016 Rmu_sc0004780.1_g000001 Rmu_sc0004805.1_g000035 Rmu_sc0010633.1_g000010 Rmu_sc0011069.1_g000003 Rmu_sc0016170.1_g000002 Rmu_sc0025757.1_g000003 Rmu_ssc0000484.1_g000003
rosa_roxburghii Rroxscaffold_2G00134200 Rroxscaffold_4G00321030 Rroxscaffold_7G00213500
rosa_rugosa Rorug01G0283100 Rorug02G0160500 Rorug02G0535300 Rorug04G0027000 Rorug04G0328100 Rorug05G0388700 Rorug05G0530200 Rorug06G0489200 Rorug07G0104900 Rorug07G0209400 Rorug07G0274500
rosa_samantha Rh2BG222800 Rh2BG618900 Rh2CG214400 Rh2CG587200 Rh6BG040200 Rh6CG206500 Rh6DG035600 Rh7BG401600 Rh7BG401700 Rh7DG418400
rosa_wichuraiana Rw2G050240 Rw6G003920 Rw7G035430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 17
Acc36I ACCTGC 1 cut(s) 149
AccII CGCG 1 cut(s) 27
AciI CCGC 4 cut(s) 130, 145, 192, 324
AclWI GGATC 2 cut(s) 253, 261
AcvI CACGTG 1 cut(s) 86
AcyI GRCGYC 1 cut(s) 189
AfaI GTAC 1 cut(s) 328
AgsI TTSAA 2 cut(s) 43, 310
AhdI GACNNNNNGTC 1 cut(s) 21
AjnI CCWGG 2 cut(s) 249, 291
AjuI GAANNNNNNNTTGG 2 cut(s) 383, 415
AluBI AGCT 2 cut(s) 268, 394
AluI AGCT 2 cut(s) 268, 394
AlwI GGATC 2 cut(s) 253, 261
Ama87I CYCGRG 1 cut(s) 104
ArsI GACNNNNNNTTYG 2 cut(s) 222, 254
AspS9I GGNCC 1 cut(s) 127
AvaI CYCGRG 1 cut(s) 104
AvaII GGWCC 1 cut(s) 127
AxyI CCTNAGG 1 cut(s) 420
BanII GRGCYC 1 cut(s) 111
BbrPI CACGTG 1 cut(s) 86
BbsI GAAGAC 1 cut(s) 298
BccI CCATC 3 cut(s) 98, 156, 230
BceAI ACGGC 1 cut(s) 299
BciT130I CCWGG 2 cut(s) 251, 293
BciVI GTATCC 1 cut(s) 420
BfaI CTAG 2 cut(s) 135, 285
BfuAI ACCTGC 1 cut(s) 149
BfuI GTATCC 1 cut(s) 420
BisI GCNGC 2 cut(s) 145, 192
BlpI GCTNAGC 1 cut(s) 5
BlsI GCNGC 2 cut(s) 146, 193
Bme1390I CCNGG 2 cut(s) 251, 293
Bme18I GGWCC 1 cut(s) 127
BmeRI GACNNNNNGTC 1 cut(s) 21
BmeT110I CYCGRG 1 cut(s) 104
BmgT120I GGNCC 1 cut(s) 127
BmiI GGNNCC 2 cut(s) 67, 290
BmrFI CCNGG 2 cut(s) 251, 293
BmrI ACTGGG 1 cut(s) 339
BmuI ACTGGG 1 cut(s) 339
BpiI GAAGAC 1 cut(s) 298
BpmI CTGGAG 3 cut(s) 52, 275, 377
Bpu10I CCTNAGC 1 cut(s) 162
Bpu1102I GCTNAGC 1 cut(s) 5
BsaAI YACGTR 1 cut(s) 86
BsaHI GRCGYC 1 cut(s) 189
BsaJI CCNNGG 1 cut(s) 292
BsaWI WCCGGW 1 cut(s) 206
BsaXI ACNNNNNCTCC 2 cut(s) 188, 218
Bse118I RCCGGY 1 cut(s) 62
Bse1I ACTGG 1 cut(s) 334
Bse21I CCTNAGG 1 cut(s) 420
BseBI CCWGG 2 cut(s) 251, 293
BseDI CCNNGG 1 cut(s) 292
BseMII CTCAG 1 cut(s) 153
BseNI ACTGG 1 cut(s) 334
BseRI GAGGAG 1 cut(s) 408
BsgI GTGCAG 1 cut(s) 265
Bsh1236I CGCG 1 cut(s) 27
Bsh1285I CGRYCG 1 cut(s) 25
BsiEI CGRYCG 1 cut(s) 25
BsiHKCI CYCGRG 1 cut(s) 104
BsiSI CCGG 2 cut(s) 63, 207
BslFI GGGAC 1 cut(s) 65
BsmFI GGGAC 1 cut(s) 65
BsoBI CYCGRG 1 cut(s) 104
Bsp1286I GDGCHC 1 cut(s) 111
Bsp143I GATC 4 cut(s) 234, 241, 253, 258
Bsp1720I GCTNAGC 1 cut(s) 5
Bsp68I TCGCGA 1 cut(s) 27
BspACI CCGC 4 cut(s) 130, 145, 192, 324
BspCNI CTCAG 1 cut(s) 154
BspFNI CGCG 1 cut(s) 27
BspLI GGNNCC 2 cut(s) 67, 290
BspMI ACCTGC 1 cut(s) 149
BspPI GGATC 2 cut(s) 253, 261
BsrFI RCCGGY 1 cut(s) 62
BsrI ACTGG 1 cut(s) 334
BssAI RCCGGY 1 cut(s) 62
BssECI CCNNGG 1 cut(s) 292
BssMI GATC 4 cut(s) 234, 241, 253, 258
BssNI GRCGYC 1 cut(s) 189
Bst2UI CCWGG 2 cut(s) 251, 293
Bst4CI ACNGT 3 cut(s) 19, 115, 406
Bst6I CTCTTC 1 cut(s) 158
BstACI GRCGYC 1 cut(s) 189
BstBAI YACGTR 1 cut(s) 86
BstC8I GCNNGC 2 cut(s) 74, 270
BstDEI CTNAG 3 cut(s) 5, 162, 420
BstFNI CGCG 1 cut(s) 27
BstKTI GATC 4 cut(s) 237, 244, 256, 261
BstMBI GATC 4 cut(s) 234, 241, 253, 258
BstMCI CGRYCG 1 cut(s) 25
BstMWI GCNNNNNNNGC 1 cut(s) 361
BstNI CCWGG 2 cut(s) 251, 293
BstNSI RCATGY 1 cut(s) 80
BstSCI CCNGG 2 cut(s) 249, 291
BstUI CGCG 1 cut(s) 27
BstV2I GAAGAC 1 cut(s) 298
BstX2I RGATCY 1 cut(s) 258
BstYI RGATCY 1 cut(s) 258
Bsu36I CCTNAGG 1 cut(s) 420
BsuI GTATCC 1 cut(s) 420
BtsIMutI CAGTG 1 cut(s) 402
BtuMI TCGCGA 1 cut(s) 27
BveI ACCTGC 1 cut(s) 149
Cac8I GCNNGC 2 cut(s) 74, 270
Cfr10I RCCGGY 1 cut(s) 62
Cfr13I GGNCC 1 cut(s) 127
CseI GACGC 1 cut(s) 197
Csp6I GTAC 1 cut(s) 327
CspCI CAANNNNNGTGG 2 cut(s) 100, 135
CviAII CATG 2 cut(s) 13, 77
CviJI RGCY 7 cut(s) 38, 66, 109, 268, 289, 364, 394
CviKI_1 RGCY 7 cut(s) 38, 66, 109, 268, 289, 364, 394
CviQI GTAC 1 cut(s) 327
DdeI CTNAG 3 cut(s) 5, 162, 420
DpnI GATC 4 cut(s) 236, 243, 255, 260
DpnII GATC 4 cut(s) 234, 241, 253, 258
DrdI GACNNNNNNGTC 1 cut(s) 17
DriI GACNNNNNGTC 1 cut(s) 21
DseDI GACNNNNNNGTC 1 cut(s) 17
Eam1104I CTCTTC 1 cut(s) 158
Eam1105I GACNNNNNGTC 1 cut(s) 21
EarI CTCTTC 1 cut(s) 158
Eco24I GRGCYC 1 cut(s) 111
Eco47I GGWCC 1 cut(s) 127
Eco72I CACGTG 1 cut(s) 86
Eco81I CCTNAGG 1 cut(s) 420
Eco88I CYCGRG 1 cut(s) 104
EcoRII CCWGG 2 cut(s) 249, 291
EcoT38I GRGCYC 1 cut(s) 111
FaeI CATG 2 cut(s) 16, 80
FaiI YATR 3 cut(s) 14, 78, 378
FaqI GGGAC 1 cut(s) 65
FatI CATG 2 cut(s) 12, 76
Fnu4HI GCNGC 2 cut(s) 145, 192
FriOI GRGCYC 1 cut(s) 111
Fsp4HI GCNGC 2 cut(s) 145, 192
FspBI CTAG 2 cut(s) 135, 285
GluI GCNGC 2 cut(s) 145, 192
GsuI CTGGAG 3 cut(s) 52, 275, 377
HapII CCGG 2 cut(s) 63, 207
HgaI GACGC 1 cut(s) 197
Hin1I GRCGYC 1 cut(s) 189
Hin1II CATG 2 cut(s) 16, 80
HindIII AAGCTT 1 cut(s) 392
HinfI GANTC 2 cut(s) 297, 399
HpaII CCGG 2 cut(s) 63, 207
Hpy166II GTNNAC 2 cut(s) 100, 373
Hpy188I TCNGA 1 cut(s) 258
Hpy188III TCNNGA 2 cut(s) 26, 262
Hpy8I GTNNAC 2 cut(s) 100, 373
Hpy99I CGWCG 5 cut(s) 23, 32, 35, 242, 320
HpyAV CCTTC 3 cut(s) 49, 58, 423
HpyCH4III ACNGT 3 cut(s) 19, 115, 406
HpyCH4IV ACGT 1 cut(s) 85
HpyCH4V TGCA 1 cut(s) 282
HpyF10VI GCNNNNNNNGC 1 cut(s) 361
HpyF3I CTNAG 3 cut(s) 5, 162, 420
HpySE526I ACGT 1 cut(s) 85
Hsp92I GRCGYC 1 cut(s) 189
Hsp92II CATG 2 cut(s) 16, 80
Kzo9I GATC 4 cut(s) 234, 241, 253, 258
LmnI GCTCC 2 cut(s) 71, 294
MaeI CTAG 2 cut(s) 135, 285
MaeII ACGT 1 cut(s) 85
MaeIII GTNAC 1 cut(s) 212
MalI GATC 4 cut(s) 236, 243, 255, 260
MboI GATC 4 cut(s) 234, 241, 253, 258
MboII GAAGA 3 cut(s) 43, 145, 298
MflI RGATCY 1 cut(s) 258
MhlI GDGCHC 1 cut(s) 111
MnlI CCTC 5 cut(s) 113, 118, 178, 354, 429
MseI TTAA 1 cut(s) 427
MspI CCGG 2 cut(s) 63, 207
MspR9I CCNGG 2 cut(s) 251, 293
MvaI CCWGG 2 cut(s) 251, 293
MvnI CGCG 1 cut(s) 27
MwoI GCNNNNNNNGC 1 cut(s) 361
NdeII GATC 4 cut(s) 234, 241, 253, 258
NlaIII CATG 2 cut(s) 16, 80
NlaIV GGNNCC 2 cut(s) 67, 290
NmuCI GTSAC 1 cut(s) 212
NruI TCGCGA 1 cut(s) 27
NspI RCATGY 1 cut(s) 80
PaeR7I CTCGAG 1 cut(s) 104
PcsI WCGNNNNNNNCGW 2 cut(s) 27, 111
PfeI GAWTC 2 cut(s) 297, 399
PkrI GCNGC 2 cut(s) 146, 193
PmaCI CACGTG 1 cut(s) 86
PmlI CACGTG 1 cut(s) 86
Ppu21I YACGTR 1 cut(s) 86
Psp6I CCWGG 2 cut(s) 249, 291
PspCI CACGTG 1 cut(s) 86
PspGI CCWGG 2 cut(s) 249, 291
PspN4I GGNNCC 2 cut(s) 67, 290
PspPI GGNCC 1 cut(s) 127
PspXI VCTCGAGB 1 cut(s) 104
PsuI RGATCY 1 cut(s) 258
RruI TCGCGA 1 cut(s) 27
RsaI GTAC 1 cut(s) 328
RsaNI GTAC 1 cut(s) 327
SaqAI TTAA 1 cut(s) 427
SatI GCNGC 2 cut(s) 145, 192
Sau3AI GATC 4 cut(s) 234, 241, 253, 258
Sau96I GGNCC 1 cut(s) 127
ScrFI CCNGG 2 cut(s) 251, 293
SduI GDGCHC 1 cut(s) 111
SetI ASST 8 cut(s) 50, 88, 105, 129, 143, 270, 396, 426
Sfr274I CTCGAG 1 cut(s) 104
SinI GGWCC 1 cut(s) 127
SlaI CTCGAG 1 cut(s) 104
SmlI CTYRAG 1 cut(s) 104
SmoI CTYRAG 1 cut(s) 104
SsiI CCGC 4 cut(s) 130, 145, 192, 324
SspMI CTAG 2 cut(s) 135, 285
StyD4I CCNGG 2 cut(s) 249, 291
TaaI ACNGT 3 cut(s) 19, 115, 406
TaiI ACGT 1 cut(s) 88
TaqI TCGA 3 cut(s) 105, 225, 240
TaqII GACCGA 1 cut(s) 11
TauI GCSGC 2 cut(s) 147, 194
TfiI GAWTC 2 cut(s) 297, 399
Tru1I TTAA 1 cut(s) 427
Tru9I TTAA 1 cut(s) 427
TscAI CASTG 1 cut(s) 409
TseFI GTSAC 1 cut(s) 212
Tsp45I GTSAC 1 cut(s) 212
TspRI CASTG 1 cut(s) 409
VpaK11BI GGWCC 1 cut(s) 127
XceI RCATGY 1 cut(s) 80
XhoI CTCGAG 1 cut(s) 104
XspI CTAG 2 cut(s) 135, 285
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.