RLG00000026518

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0000549
Physical Location & Seq
Reverse (-)
3543092 .. 3543598
507 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000026518

Sequence Viewer

Length: 507 bp
ATGCAAAGTGACAAGACCGTAGGAGGCAGAGACGACGCCTTCAACACTTTCTTCAGTGAAATTGGAGCCGGATTGCACATCCCCCGCACCGTGCCCCTGGATCTGGAGCCCACCGTCGTCAACGAGGTGAGGACCGGAACTTACCGGCTGTTCCACCCCGAGCAGCTCATCAGCAGCAAAGAGGACGTCGTCGATAACTTCGCCAGAGGCCATTACACAATTGGGAAGGAGATTGTGGATCGGATCCGGAAGCTTGCGGACAACTGTACTGGGCTTCAAGGGTTTCTGGTGCCCCCATGCCGTCGGAGGTGGCTTGGATCTCTGCTCTTGGAGAGGCTCTCTGTGGACTATGGGAAGAAATCTAAGCTTGGATTCACTGTTTACCCATCTCCTCAGGTCTCAACCTCTGTTGTTGAGCCTTATAATAATGTTTTGTCTACTCGCTCGCTCTGGGAGCACACTGATGTGGCCGTGCTTCTGGACAATGAAGCCATCTATGATATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04145 ko04210 ko04530 ko04540 ko05130 map04145 map04210 map04530 map04540 map05130
Pfam Domains
Protein Families
Tubulin Tubulin_C

Protein Analysis

169

Amino Acids

18.91

Weight (kDa)

5.8

Isoelectric Point (pI)

42.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tubulin PF00091 13 - 97 1.2e-13 Tubulin/FtsZ family, GTPase domain
Tubulin PF00091 105 - 168 5.3e-11 Tubulin/FtsZ family, GTPase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000549)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G04820 AT1G50010 AT4G14960 AT4G14960
fragaria_vesca FvH4_1g18660 FvH4_2g03140 FvH4_5g33560 FvH4_6g48940 FvH4_6g48940
malus_domestica MD02G1204800.v1.1 MD05G1106800.v1.1 MD08G1210600.v1.1 MD09G1049500.v1.1 MD10G1111600.v1.1 MD15G1398700.v1.1 MD17G1049700.v1.1
prunus_persica Prupe.1G542600_v2.0.a1 Prupe.3G269300_v2.0.a1 Prupe.6G212700_v2.0.a1 Prupe.8G150900_v2.0.a1
pyrus_communis pycom05g10370 pycom08g18150 pycom10g09640 pycom10g09660 pycom111g04050 pycom15g35470 pycom17g04460
rosa_chinensis RchiOBHm_Chr2g0109481 RchiOBHm_Chr2g0168751 RchiOBHm_Chr3g0453071 RchiOBHm_Chr3g0460741 RchiOBHm_Chr3g0467971 RchiOBHm_Chr4g0400901 RchiOBHm_Chr6g0248011 RchiOBHm_Chr7g0232381 RchiOBHm_Chr7g0238121
rosa_laevigata RLG00000001350 RLG00000009167 RLG00000015163 RLG00000017708 RLG00000017741 RLG00000021807 RLG00000023849 RLG00000026518
rosa_multiflora Rmu_sc0000332.1_g000021 Rmu_sc0000332.1_g000029 Rmu_sc0002416.1_g000007 Rmu_sc0002611.1_g000001 Rmu_sc0003113.1_g000016 Rmu_sc0004780.1_g000001 Rmu_sc0004805.1_g000035 Rmu_sc0010633.1_g000010 Rmu_sc0011069.1_g000003 Rmu_sc0016170.1_g000002 Rmu_sc0025757.1_g000003 Rmu_ssc0000484.1_g000003
rosa_roxburghii Rroxscaffold_2G00134200 Rroxscaffold_4G00321030 Rroxscaffold_7G00213500
rosa_rugosa Rorug01G0283100 Rorug02G0160500 Rorug02G0535300 Rorug04G0027000 Rorug04G0328100 Rorug05G0388700 Rorug05G0530200 Rorug06G0489200 Rorug07G0104900 Rorug07G0209400 Rorug07G0274500
rosa_samantha Rh2BG222800 Rh2BG618900 Rh2CG214400 Rh2CG587200 Rh6BG040200 Rh6CG206500 Rh6DG035600 Rh7BG401600 Rh7BG401700 Rh7DG418400
rosa_wichuraiana Rw2G050240 Rw6G003920 Rw7G035430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 423
AatII GACGTC 1 cut(s) 189
AccB1I GGYRCC 1 cut(s) 289
AccI GTMKAC 1 cut(s) 437
AccIII TCCGGA 1 cut(s) 246
AciI CCGC 2 cut(s) 85, 257
AclWI GGATC 5 cut(s) 108, 238, 246, 251, 325
AcoI YGGCCR 1 cut(s) 468
AcuI CTGAAG 1 cut(s) 37
AcyI GRCGYC 2 cut(s) 36, 186
AfaI GTAC 1 cut(s) 268
AfiI CCNNNNNNNGG 1 cut(s) 103
AgsI TTSAA 2 cut(s) 43, 278
AjnI CCWGG 1 cut(s) 96
AleI CACNNNNGTG 1 cut(s) 464
AluBI AGCT 3 cut(s) 166, 253, 367
AluI AGCT 3 cut(s) 166, 253, 367
Alw21I GWGCWC 1 cut(s) 459
Alw26I GTCTC 2 cut(s) 24, 403
AlwI GGATC 5 cut(s) 108, 238, 246, 251, 325
Ama87I CYCGRG 1 cut(s) 158
Aor13HI TCCGGA 1 cut(s) 246
AoxI GGCC 2 cut(s) 208, 468
ApeKI GCWGC 2 cut(s) 163, 174
ArsI GACNNNNNNTTYG 2 cut(s) 171, 203
AspS9I GGNCC 1 cut(s) 132
AsuHPI GGTGA 1 cut(s) 139
AvaI CYCGRG 1 cut(s) 158
AvaII GGWCC 1 cut(s) 132
AxyI CCTNAGG 1 cut(s) 393
BaeGI GKGCMC 2 cut(s) 96, 294
BamHI GGATCC 1 cut(s) 243
BanI GGYRCC 1 cut(s) 289
BanII GRGCYC 1 cut(s) 111
Bbv12I GWGCWC 1 cut(s) 459
BbvI GCAGC 2 cut(s) 175, 186
BccI CCATC 2 cut(s) 394, 500
BceAI ACGGC 2 cut(s) 285, 455
BciT130I CCWGG 1 cut(s) 98
BcoDI GTCTC 2 cut(s) 24, 403
BisI GCNGC 2 cut(s) 164, 175
BlsI GCNGC 2 cut(s) 165, 176
Bme1390I CCNGG 1 cut(s) 98
Bme18I GGWCC 1 cut(s) 132
BmeT110I CYCGRG 1 cut(s) 158
BmgT120I GGNCC 1 cut(s) 132
BmiI GGNNCC 4 cut(s) 67, 108, 245, 291
BmrFI CCNGG 1 cut(s) 98
BmrI ACTGGG 1 cut(s) 279
BmuI ACTGGG 1 cut(s) 279
BplI GAGNNNNNCTC 2 cut(s) 323, 355
BpmI CTGGAG 1 cut(s) 125
BsaHI GRCGYC 2 cut(s) 36, 186
BsaI GGTCTC 1 cut(s) 403
BsaJI CCNNGG 1 cut(s) 96
BsaWI WCCGGW 2 cut(s) 134, 246
Bsc4I CCNNNNNNNGG 1 cut(s) 103
Bse118I RCCGGY 1 cut(s) 144
Bse1I ACTGG 1 cut(s) 274
Bse21I CCTNAGG 1 cut(s) 393
BseAI TCCGGA 1 cut(s) 246
BseBI CCWGG 1 cut(s) 98
BseDI CCNNGG 1 cut(s) 96
BseGI GGATG 1 cut(s) 78
BseLI CCNNNNNNNGG 1 cut(s) 103
BseMII CTCAG 1 cut(s) 407
BseNI ACTGG 1 cut(s) 274
BseRI GAGGAG 1 cut(s) 381
BseSI GKGCMC 2 cut(s) 96, 294
BseXI GCAGC 2 cut(s) 175, 186
BshFI GGCC 2 cut(s) 210, 470
BshNI GGYRCC 1 cut(s) 289
BsiHKAI GWGCWC 1 cut(s) 459
BsiHKCI CYCGRG 1 cut(s) 158
BsiSI CCGG 4 cut(s) 69, 135, 145, 247
BslI CCNNNNNNNGG 1 cut(s) 103
BsmAI GTCTC 2 cut(s) 24, 403
BsmBI CGTCTC 1 cut(s) 24
BsnI GGCC 2 cut(s) 210, 470
Bso31I GGTCTC 1 cut(s) 403
BsoBI CYCGRG 1 cut(s) 158
Bsp1286I GDGCHC 4 cut(s) 96, 111, 294, 459
Bsp13I TCCGGA 1 cut(s) 246
Bsp143I GATC 4 cut(s) 100, 238, 243, 317
BspACI CCGC 2 cut(s) 85, 257
BspANI GGCC 2 cut(s) 210, 470
BspCNI CTCAG 1 cut(s) 406
BspEI TCCGGA 1 cut(s) 246
BspLI GGNNCC 4 cut(s) 67, 108, 245, 291
BspPI GGATC 5 cut(s) 108, 238, 246, 251, 325
BspT107I GGYRCC 1 cut(s) 289
BspTNI GGTCTC 1 cut(s) 403
BsrFI RCCGGY 1 cut(s) 144
BsrI ACTGG 1 cut(s) 274
BssAI RCCGGY 1 cut(s) 144
BssECI CCNNGG 1 cut(s) 96
BssMI GATC 4 cut(s) 100, 238, 243, 317
BssNI GRCGYC 2 cut(s) 36, 186
Bst2UI CCWGG 1 cut(s) 98
Bst4CI ACNGT 5 cut(s) 19, 91, 115, 266, 379
BstACI GRCGYC 2 cut(s) 36, 186
BstC8I GCNNGC 2 cut(s) 255, 446
BstDEI CTNAG 2 cut(s) 363, 393
BstF5I GGATG 1 cut(s) 78
BstKTI GATC 4 cut(s) 103, 241, 246, 320
BstMAI GTCTC 2 cut(s) 24, 403
BstMBI GATC 4 cut(s) 100, 238, 243, 317
BstMWI GCNNNNNNNGC 1 cut(s) 454
BstNI CCWGG 1 cut(s) 98
BstSCI CCNGG 1 cut(s) 96
BstSLI GKGCMC 2 cut(s) 96, 294
BstV1I GCAGC 2 cut(s) 175, 186
BstX2I RGATCY 3 cut(s) 100, 243, 317
BstYI RGATCY 3 cut(s) 100, 243, 317
Bsu36I CCTNAGG 1 cut(s) 393
BsuRI GGCC 2 cut(s) 210, 470
BtsCI GGATG 1 cut(s) 78
BtsIMutI CAGTG 3 cut(s) 61, 375, 459
Cac8I GCNNGC 2 cut(s) 255, 446
Cfr10I RCCGGY 1 cut(s) 144
Cfr13I GGNCC 1 cut(s) 132
CseI GACGC 1 cut(s) 44
Csp6I GTAC 1 cut(s) 267
CviAII CATG 1 cut(s) 297
CviQI GTAC 1 cut(s) 267
DdeI CTNAG 2 cut(s) 363, 393
DpnI GATC 4 cut(s) 102, 240, 245, 319
DpnII GATC 4 cut(s) 100, 238, 243, 317
EaeI YGGCCR 1 cut(s) 468
Eco24I GRGCYC 1 cut(s) 111
Eco31I GGTCTC 1 cut(s) 403
Eco32I GATATC 1 cut(s) 502
Eco47I GGWCC 1 cut(s) 132
Eco57I CTGAAG 1 cut(s) 37
Eco81I CCTNAGG 1 cut(s) 393
Eco88I CYCGRG 1 cut(s) 158
EcoRII CCWGG 1 cut(s) 96
EcoRV GATATC 1 cut(s) 502
EcoT38I GRGCYC 1 cut(s) 111
Esp3I CGTCTC 1 cut(s) 24
FaeI CATG 1 cut(s) 300
FaiI YATR 4 cut(s) 298, 351, 423, 498
FatI CATG 1 cut(s) 296
FauI CCCGC 1 cut(s) 92
FblI GTMKAC 1 cut(s) 437
Fnu4HI GCNGC 2 cut(s) 164, 175
FokI GGATG 1 cut(s) 65
FriOI GRGCYC 1 cut(s) 111
Fsp4HI GCNGC 2 cut(s) 164, 175
GluI GCNGC 2 cut(s) 164, 175
GsuI CTGGAG 1 cut(s) 125
HaeIII GGCC 2 cut(s) 210, 470
HapII CCGG 4 cut(s) 69, 135, 145, 247
HgaI GACGC 1 cut(s) 44
Hin1I GRCGYC 2 cut(s) 36, 186
Hin1II CATG 1 cut(s) 300
HincII GTYRAC 1 cut(s) 121
HindII GTYRAC 1 cut(s) 121
HindIII AAGCTT 2 cut(s) 251, 365
HinfI GANTC 1 cut(s) 372
HpaII CCGG 4 cut(s) 69, 135, 145, 247
HphI GGTGA 1 cut(s) 139
Hpy166II GTNNAC 4 cut(s) 121, 346, 382, 438
Hpy188I TCNGA 3 cut(s) 243, 306, 506
Hpy188III TCNNGA 3 cut(s) 104, 247, 479
Hpy8I GTNNAC 4 cut(s) 121, 346, 382, 438
Hpy99I CGWCG 5 cut(s) 38, 119, 191, 194, 306
HpyAV CCTTC 2 cut(s) 49, 220
HpyCH4III ACNGT 5 cut(s) 19, 91, 115, 266, 379
HpyCH4IV ACGT 1 cut(s) 186
HpyCH4V TGCA 2 cut(s) 4, 76
HpyF10VI GCNNNNNNNGC 1 cut(s) 454
HpyF3I CTNAG 2 cut(s) 363, 393
HpySE526I ACGT 1 cut(s) 186
Hsp92I GRCGYC 2 cut(s) 36, 186
Hsp92II CATG 1 cut(s) 300
Kpn2I TCCGGA 1 cut(s) 246
Kzo9I GATC 4 cut(s) 100, 238, 243, 317
LmnI GCTCC 3 cut(s) 65, 106, 454
Lsp1109I GCAGC 2 cut(s) 175, 186
MaeII ACGT 1 cut(s) 186
MaeIII GTNAC 1 cut(s) 8
MalI GATC 4 cut(s) 102, 240, 245, 319
MboI GATC 4 cut(s) 100, 238, 243, 317
MboII GAAGA 2 cut(s) 43, 367
MfeI CAATTG 1 cut(s) 219
MflI RGATCY 3 cut(s) 100, 243, 317
MhlI GDGCHC 4 cut(s) 96, 111, 294, 459
MluCI AATT 2 cut(s) 60, 219
MmeI TCCRAC 1 cut(s) 284
MnlI CCTC 9 cut(s) 17, 118, 123, 175, 200, 300, 327, 402, 415
MroI TCCGGA 1 cut(s) 246
MslI CAYNNNNRTG 2 cut(s) 462, 464
MspI CCGG 4 cut(s) 69, 135, 145, 247
MspR9I CCNGG 1 cut(s) 98
MunI CAATTG 1 cut(s) 219
MvaI CCWGG 1 cut(s) 98
MwoI GCNNNNNNNGC 1 cut(s) 454
NdeII GATC 4 cut(s) 100, 238, 243, 317
NlaIII CATG 1 cut(s) 300
NlaIV GGNNCC 4 cut(s) 67, 108, 245, 291
NmuCI GTSAC 1 cut(s) 8
OliI CACNNNNGTG 1 cut(s) 464
PfeI GAWTC 1 cut(s) 372
PflFI GACNNNGTC 1 cut(s) 188
PkrI GCNGC 2 cut(s) 165, 176
PsiI TTATAA 1 cut(s) 423
Psp6I CCWGG 1 cut(s) 96
PspGI CCWGG 1 cut(s) 96
PspN4I GGNNCC 4 cut(s) 67, 108, 245, 291
PspPI GGNCC 1 cut(s) 132
PsuI RGATCY 3 cut(s) 100, 243, 317
PsyI GACNNNGTC 1 cut(s) 188
RsaI GTAC 1 cut(s) 268
RsaNI GTAC 1 cut(s) 267
RseI CAYNNNNRTG 2 cut(s) 462, 464
SatI GCNGC 2 cut(s) 164, 175
Sau3AI GATC 4 cut(s) 100, 238, 243, 317
Sau96I GGNCC 1 cut(s) 132
ScrFI CCNGG 1 cut(s) 98
SduI GDGCHC 4 cut(s) 96, 111, 294, 459
SetI ASST 8 cut(s) 129, 168, 189, 255, 311, 369, 399, 407
SinI GGWCC 1 cut(s) 132
SmiMI CAYNNNNRTG 2 cut(s) 462, 464
Sse9I AATT 2 cut(s) 60, 219
SsiI CCGC 2 cut(s) 85, 257
StyD4I CCNGG 1 cut(s) 96
TaaI ACNGT 5 cut(s) 19, 91, 115, 266, 379
TaiI ACGT 1 cut(s) 189
TaqI TCGA 1 cut(s) 192
TasI AATT 2 cut(s) 60, 219
TatI WGTACW 1 cut(s) 266
TfiI GAWTC 1 cut(s) 372
TscAI CASTG 3 cut(s) 61, 382, 466
TseFI GTSAC 1 cut(s) 8
TseI GCWGC 2 cut(s) 163, 174
Tsp45I GTSAC 1 cut(s) 8
TspDTI ATGAA 1 cut(s) 501
TspRI CASTG 3 cut(s) 61, 382, 466
Tth111I GACNNNGTC 1 cut(s) 188
VpaK11BI GGWCC 1 cut(s) 132
XcmI CCANNNNNNNNNTGG 1 cut(s) 218
XmiI GTMKAC 1 cut(s) 437
ZraI GACGTC 1 cut(s) 187
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.