Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 1 cut(s) | 122 |
| Acc65I | GGTACC | 1 cut(s) | 431 |
| AccB1I | GGYRCC | 5 cut(s) | 170, 431, 970, 1038, 1307 |
| AccI | GTMKAC | 1 cut(s) | 204 |
| AccII | CGCG | 4 cut(s) | 132, 190, 192, 315 |
| AccIII | TCCGGA | 1 cut(s) | 366 |
| AcoI | YGGCCR | 6 cut(s) | 28, 100, 127, 316, 906, 996 |
| AcyI | GRCGYC | 3 cut(s) | 141, 171, 294 |
| AfaI | GTAC | 6 cut(s) | 433, 935, 956, 1018, 1196, 1352 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 649, 726, 926, 1108 |
| AgsI | TTSAA | 3 cut(s) | 148, 415, 1054 |
| AjiI | CACGTC | 1 cut(s) | 184 |
| AjnI | CCWGG | 8 cut(s) | 30, 42, 354, 396, 405, 525, 648, 1090 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 488, 520 |
| AleI | CACNNNNGTG | 2 cut(s) | 596, 1148 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 1235, 1267 |
| AluBI | AGCT | 7 cut(s) | 67, 256, 274, 373, 499, 856, 896 |
| AluI | AGCT | 7 cut(s) | 67, 256, 274, 373, 499, 856, 896 |
| Alw21I | GWGCWC | 2 cut(s) | 567, 591 |
| Alw26I | GTCTC | 5 cut(s) | 80, 158, 449, 697, 1338 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 866 |
| Ama87I | CYCGRG | 4 cut(s) | 77, 209, 266, 446 |
| Aor13HI | TCCGGA | 1 cut(s) | 366 |
| ApeKI | GCWGC | 4 cut(s) | 271, 580, 853, 1277 |
| Asp718I | GGTACC | 1 cut(s) | 431 |
| AspLEI | GCGC | 3 cut(s) | 173, 192, 194 |
| AspS9I | GGNCC | 2 cut(s) | 232, 1261 |
| AsuC2I | CCSGG | 2 cut(s) | 391, 436 |
| AsuHPI | GGTGA | 5 cut(s) | 712, 865, 1109, 1241, 1346 |
| AvaI | CYCGRG | 4 cut(s) | 77, 209, 266, 446 |
| AvaII | GGWCC | 1 cut(s) | 232 |
| AxyI | CCTNAGG | 2 cut(s) | 725, 975 |
| BaeGI | GKGCMC | 1 cut(s) | 1043 |
| BalI | TGGCCA | 2 cut(s) | 908, 998 |
| BamHI | GGATCC | 3 cut(s) | 87, 363, 792 |
| BanI | GGYRCC | 5 cut(s) | 170, 431, 970, 1038, 1307 |
| BanII | GRGCYC | 3 cut(s) | 216, 396, 742 |
| BauI | CACGAG | 1 cut(s) | 848 |
| BbsI | GAAGAC | 1 cut(s) | 403 |
| Bbv12I | GWGCWC | 2 cut(s) | 567, 591 |
| BbvCI | CCTCAGC | 2 cut(s) | 1155, 1320 |
| BbvI | GCAGC | 4 cut(s) | 283, 567, 865, 1264 |
| BccI | CCATC | 6 cut(s) | 203, 335, 728, 898, 1010, 1325 |
| BceAI | ACGGC | 3 cut(s) | 115, 404, 732 |
| BciT130I | CCWGG | 8 cut(s) | 32, 44, 356, 398, 407, 527, 650, 1092 |
| BclI | TGATCA | 1 cut(s) | 1174 |
| BcnI | CCSGG | 2 cut(s) | 391, 436 |
| BcoDI | GTCTC | 5 cut(s) | 80, 158, 449, 697, 1338 |
| BfmI | CTRYAG | 1 cut(s) | 831 |
| BfoI | RGCGCY | 1 cut(s) | 174 |
| BmcAI | AGTACT | 1 cut(s) | 1352 |
| Bme18I | GGWCC | 1 cut(s) | 232 |
| BmeT110I | CYCGRG | 4 cut(s) | 77, 209, 266, 446 |
| BmgBI | CACGTC | 1 cut(s) | 184 |
| BmgT120I | GGNCC | 2 cut(s) | 232, 1261 |
| BmrI | ACTGGG | 1 cut(s) | 1056 |
| BmsI | GCATC | 1 cut(s) | 96 |
| BmuI | ACTGGG | 1 cut(s) | 1056 |
| BpiI | GAAGAC | 1 cut(s) | 403 |
| BplI | GAGNNNNNCTC | 4 cut(s) | 842, 874, 1148, 1180 |
| BpmI | CTGGAG | 3 cut(s) | 380, 482, 1304 |
| Bpu10I | CCTNAGC | 3 cut(s) | 1107, 1155, 1320 |
| BpuEI | CTTGAG | 1 cut(s) | 605 |
| BpuMI | CCSGG | 2 cut(s) | 391, 436 |
| BsaBI | GATNNNNATC | 1 cut(s) | 23 |
| BsaHI | GRCGYC | 3 cut(s) | 141, 171, 294 |
| BsaI | GGTCTC | 2 cut(s) | 158, 449 |
| BsaJI | CCNNGG | 7 cut(s) | 130, 397, 525, 648, 788, 925, 958 |
| BsaWI | WCCGGW | 2 cut(s) | 239, 366 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 954, 984 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 649, 726, 926, 1108 |
| Bse118I | RCCGGY | 2 cut(s) | 167, 173 |
| Bse1I | ACTGG | 3 cut(s) | 1040, 1051, 1146 |
| Bse21I | CCTNAGG | 2 cut(s) | 725, 975 |
| Bse8I | GATNNNNATC | 1 cut(s) | 23 |
| BseAI | TCCGGA | 1 cut(s) | 366 |
| BseBI | CCWGG | 8 cut(s) | 32, 44, 356, 398, 407, 527, 650, 1092 |
| BseDI | CCNNGG | 7 cut(s) | 130, 397, 525, 648, 788, 925, 958 |
| BseGI | GGATG | 2 cut(s) | 757, 985 |
| BseJI | GATNNNNATC | 1 cut(s) | 23 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 649, 726, 926, 1108 |
| BseMII | CTCAG | 8 cut(s) | 710, 716, 750, 858, 873, 1146, 1248, 1311 |
| BseNI | ACTGG | 3 cut(s) | 1040, 1051, 1146 |
| BsePI | GCGCGC | 1 cut(s) | 190 |
| BseRI | GAGGAG | 3 cut(s) | 799, 975, 1313 |
| BseSI | GKGCMC | 1 cut(s) | 1043 |
| BseX3I | CGGCCG | 1 cut(s) | 127 |
| BseXI | GCAGC | 4 cut(s) | 283, 567, 865, 1264 |
| BseYI | CCCAGC | 1 cut(s) | 60 |
| BsgI | GTGCAG | 2 cut(s) | 370, 1133 |
| Bsh1236I | CGCG | 4 cut(s) | 132, 190, 192, 315 |
| Bsh1285I | CGRYCG | 1 cut(s) | 130 |
| BshNI | GGYRCC | 5 cut(s) | 170, 431, 970, 1038, 1307 |
| BsiEI | CGRYCG | 1 cut(s) | 130 |
| BsiHKAI | GWGCWC | 2 cut(s) | 567, 591 |
| BsiHKCI | CYCGRG | 4 cut(s) | 77, 209, 266, 446 |
| BsiSI | CCGG | 9 cut(s) | 36, 95, 168, 174, 240, 282, 367, 390, 435 |
| BslFI | GGGAC | 2 cut(s) | 170, 648 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 649, 726, 926, 1108 |
| BsmAI | GTCTC | 5 cut(s) | 80, 158, 449, 697, 1338 |
| BsmBI | CGTCTC | 1 cut(s) | 1338 |
| BsmFI | GGGAC | 2 cut(s) | 170, 648 |
| BsmI | GAATGC | 1 cut(s) | 991 |
| Bso31I | GGTCTC | 2 cut(s) | 158, 449 |
| BsoBI | CYCGRG | 4 cut(s) | 77, 209, 266, 446 |
| Bsp1286I | GDGCHC | 6 cut(s) | 216, 396, 567, 591, 742, 1043 |
| Bsp13I | TCCGGA | 1 cut(s) | 366 |
| Bsp19I | CCATGG | 1 cut(s) | 925 |
| BspCNI | CTCAG | 8 cut(s) | 709, 717, 751, 859, 872, 1147, 1249, 1312 |
| BspEI | TCCGGA | 1 cut(s) | 366 |
| BspFNI | CGCG | 4 cut(s) | 132, 190, 192, 315 |
| BspMAI | CTGCAG | 1 cut(s) | 835 |
| BspQI | GCTCTTC | 1 cut(s) | 263 |
| BspT107I | GGYRCC | 5 cut(s) | 170, 431, 970, 1038, 1307 |
| BspTNI | GGTCTC | 2 cut(s) | 158, 449 |
| BsrFI | RCCGGY | 2 cut(s) | 167, 173 |
| BsrI | ACTGG | 3 cut(s) | 1040, 1051, 1146 |
| BssAI | RCCGGY | 2 cut(s) | 167, 173 |
| BssECI | CCNNGG | 7 cut(s) | 130, 397, 525, 648, 788, 925, 958 |
| BssHII | GCGCGC | 1 cut(s) | 190 |
| BssNI | GRCGYC | 3 cut(s) | 141, 171, 294 |
| BssSI | CACGAG | 1 cut(s) | 848 |
| BssT1I | CCWWGG | 2 cut(s) | 788, 925 |
| Bst2BI | CACGAG | 1 cut(s) | 848 |
| Bst2UI | CCWGG | 8 cut(s) | 32, 44, 356, 398, 407, 527, 650, 1092 |
| Bst4CI | ACNGT | 7 cut(s) | 74, 124, 220, 511, 560, 881, 1084 |
| Bst6I | CTCTTC | 1 cut(s) | 263 |
| BstACI | GRCGYC | 3 cut(s) | 141, 171, 294 |
| BstDSI | CCRYGG | 2 cut(s) | 130, 925 |
| BstEII | GGTNACC | 1 cut(s) | 1097 |
| BstENI | CCTNNNNNAGG | 2 cut(s) | 724, 1106 |
| BstF5I | GGATG | 2 cut(s) | 757, 985 |
| BstFNI | CGCG | 4 cut(s) | 132, 190, 192, 315 |
| BstH2I | RGCGCY | 1 cut(s) | 174 |
| BstHHI | GCGC | 3 cut(s) | 173, 192, 194 |
| BstMAI | GTCTC | 5 cut(s) | 80, 158, 449, 697, 1338 |
| BstMCI | CGRYCG | 1 cut(s) | 130 |
| BstMWI | GCNNNNNNNGC | 7 cut(s) | 187, 466, 586, 839, 995, 1205, 1316 |
| BstNI | CCWGG | 8 cut(s) | 32, 44, 356, 398, 407, 527, 650, 1092 |
| BstPI | GGTNACC | 1 cut(s) | 1097 |
| BstSFI | CTRYAG | 1 cut(s) | 831 |
| BstSLI | GKGCMC | 1 cut(s) | 1043 |
| BstUI | CGCG | 4 cut(s) | 132, 190, 192, 315 |
| BstV1I | GCAGC | 4 cut(s) | 283, 567, 865, 1264 |
| BstV2I | GAAGAC | 1 cut(s) | 403 |
| BstX2I | RGATCY | 4 cut(s) | 87, 363, 792, 1270 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 1150 |
| BstYI | RGATCY | 4 cut(s) | 87, 363, 792, 1270 |
| BstZI | CGGCCG | 1 cut(s) | 127 |
| Bsu36I | CCTNAGG | 2 cut(s) | 725, 975 |
| BtgI | CCRYGG | 2 cut(s) | 130, 925 |
| BtrI | CACGTC | 1 cut(s) | 184 |
| BtsCI | GGATG | 2 cut(s) | 757, 985 |
| BtsIMutI | CAGTG | 6 cut(s) | 507, 565, 867, 886, 1080, 1153 |
| CaiI | CAGNNNCTG | 1 cut(s) | 866 |
| CfoI | GCGC | 3 cut(s) | 173, 192, 194 |
| Cfr10I | RCCGGY | 2 cut(s) | 167, 173 |
| Cfr13I | GGNCC | 2 cut(s) | 232, 1261 |
| Cfr42I | CCGCGG | 1 cut(s) | 133 |
| CseI | GACGC | 3 cut(s) | 149, 302, 650 |
| Csp6I | GTAC | 6 cut(s) | 432, 934, 955, 1017, 1195, 1351 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 523, 558 |
| CviAII | CATG | 7 cut(s) | 83, 802, 901, 926, 937, 1129, 1237 |
| CviQI | GTAC | 6 cut(s) | 432, 934, 955, 1017, 1195, 1351 |
| DinI | GGCGCC | 1 cut(s) | 172 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 122 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 122 |
| EaeI | YGGCCR | 6 cut(s) | 28, 100, 127, 316, 906, 996 |
| EagI | CGGCCG | 1 cut(s) | 127 |
| Eam1104I | CTCTTC | 1 cut(s) | 263 |
| EarI | CTCTTC | 1 cut(s) | 263 |
| EclXI | CGGCCG | 1 cut(s) | 127 |
| Eco130I | CCWWGG | 2 cut(s) | 788, 925 |
| Eco24I | GRGCYC | 3 cut(s) | 216, 396, 742 |
| Eco31I | GGTCTC | 2 cut(s) | 158, 449 |
| Eco47I | GGWCC | 1 cut(s) | 232 |
| Eco52I | CGGCCG | 1 cut(s) | 127 |
| Eco81I | CCTNAGG | 2 cut(s) | 725, 975 |
| Eco88I | CYCGRG | 4 cut(s) | 77, 209, 266, 446 |
| Eco91I | GGTNACC | 1 cut(s) | 1097 |
| EcoNI | CCTNNNNNAGG | 2 cut(s) | 724, 1106 |
| EcoO65I | GGTNACC | 1 cut(s) | 1097 |
| EcoRII | CCWGG | 8 cut(s) | 30, 42, 354, 396, 405, 525, 648, 1090 |
| EcoT14I | CCWWGG | 2 cut(s) | 788, 925 |
| EcoT38I | GRGCYC | 3 cut(s) | 216, 396, 742 |
| EgeI | GGCGCC | 1 cut(s) | 172 |
| EheI | GGCGCC | 1 cut(s) | 172 |
| ErhI | CCWWGG | 2 cut(s) | 788, 925 |
| Esp3I | CGTCTC | 1 cut(s) | 1338 |
| FaeI | CATG | 7 cut(s) | 86, 805, 904, 929, 940, 1132, 1240 |
| FaqI | GGGAC | 2 cut(s) | 170, 648 |
| FatI | CATG | 7 cut(s) | 82, 801, 900, 925, 936, 1128, 1236 |
| FauI | CCCGC | 2 cut(s) | 195, 1175 |
| FbaI | TGATCA | 1 cut(s) | 1174 |
| FblI | GTMKAC | 1 cut(s) | 204 |
| FokI | GGATG | 2 cut(s) | 764, 992 |
| FriOI | GRGCYC | 3 cut(s) | 216, 396, 742 |
| GlaI | GCGC | 3 cut(s) | 172, 191, 193 |
| GsaI | CCCAGC | 1 cut(s) | 64 |
| GsuI | CTGGAG | 3 cut(s) | 380, 482, 1304 |
| HaeII | RGCGCY | 1 cut(s) | 174 |
| HapII | CCGG | 9 cut(s) | 36, 95, 168, 174, 240, 282, 367, 390, 435 |
| HgaI | GACGC | 3 cut(s) | 149, 302, 650 |
| HhaI | GCGC | 3 cut(s) | 173, 192, 194 |
| Hin1I | GRCGYC | 3 cut(s) | 141, 171, 294 |
| Hin1II | CATG | 7 cut(s) | 86, 805, 904, 929, 940, 1132, 1240 |
| Hin6I | GCGC | 3 cut(s) | 171, 190, 192 |
| HinP1I | GCGC | 3 cut(s) | 171, 190, 192 |
| HincII | GTYRAC | 1 cut(s) | 205 |
| HindII | GTYRAC | 1 cut(s) | 205 |
| HinfI | GANTC | 4 cut(s) | 39, 402, 504, 1313 |
| HpaII | CCGG | 9 cut(s) | 36, 95, 168, 174, 240, 282, 367, 390, 435 |
| HphI | GGTGA | 5 cut(s) | 712, 865, 1109, 1241, 1346 |
| Hpy166II | GTNNAC | 3 cut(s) | 205, 478, 1033 |
| Hpy188I | TCNGA | 7 cut(s) | 50, 163, 363, 503, 744, 1191, 1258 |
| Hpy188III | TCNNGA | 6 cut(s) | 77, 331, 367, 766, 1006, 1283 |
| Hpy8I | GTNNAC | 3 cut(s) | 205, 478, 1033 |
| Hpy99I | CGWCG | 7 cut(s) | 128, 140, 143, 206, 230, 347, 425 |
| HpyAV | CCTTC | 6 cut(s) | 154, 163, 528, 1238, 1282, 1334 |
| HpyCH4III | ACNGT | 7 cut(s) | 74, 124, 220, 511, 560, 881, 1084 |
| HpyCH4IV | ACGT | 1 cut(s) | 183 |
| HpyCH4V | TGCA | 6 cut(s) | 12, 387, 833, 1114, 1128, 1216 |
| HpyF10VI | GCNNNNNNNGC | 7 cut(s) | 187, 466, 586, 839, 995, 1205, 1316 |
| HpySE526I | ACGT | 1 cut(s) | 183 |
| Hsp92I | GRCGYC | 3 cut(s) | 141, 171, 294 |
| Hsp92II | CATG | 7 cut(s) | 86, 805, 904, 929, 940, 1132, 1240 |
| HspAI | GCGC | 3 cut(s) | 171, 190, 192 |
| KasI | GGCGCC | 1 cut(s) | 170 |
| Kpn2I | TCCGGA | 1 cut(s) | 366 |
| KpnI | GGTACC | 1 cut(s) | 435 |
| KroI | GCCGGC | 1 cut(s) | 173 |
| KroNI | GCCGGC | 1 cut(s) | 175 |
| Ksp22I | TGATCA | 1 cut(s) | 1174 |
| KspI | CCGCGG | 1 cut(s) | 133 |
| LguI | GCTCTTC | 1 cut(s) | 263 |
| LmnI | GCTCC | 4 cut(s) | 64, 399, 547, 823 |
| Lsp1109I | GCAGC | 4 cut(s) | 283, 567, 865, 1264 |
| LweI | GCATC | 1 cut(s) | 96 |
| MaeII | ACGT | 1 cut(s) | 183 |
| MaeIII | GTNAC | 2 cut(s) | 754, 1097 |
| MboII | GAAGA | 4 cut(s) | 148, 190, 250, 403 |
| MflI | RGATCY | 4 cut(s) | 87, 363, 792, 1270 |
| MhlI | GDGCHC | 6 cut(s) | 216, 396, 567, 591, 742, 1043 |
| MlsI | TGGCCA | 2 cut(s) | 908, 998 |
| MluNI | TGGCCA | 2 cut(s) | 908, 998 |
| Mly113I | GGCGCC | 1 cut(s) | 171 |
| MlyI | GAGTC | 1 cut(s) | 1322 |
| MmeI | TCCRAC | 2 cut(s) | 28, 1161 |
| Mox20I | TGGCCA | 2 cut(s) | 908, 998 |
| MroI | TCCGGA | 1 cut(s) | 366 |
| MroNI | GCCGGC | 1 cut(s) | 173 |
| MscI | TGGCCA | 2 cut(s) | 908, 998 |
| MslI | CAYNNNNRTG | 4 cut(s) | 594, 596, 1148, 1221 |
| Msp20I | TGGCCA | 2 cut(s) | 908, 998 |
| MspA1I | CMGCKG | 1 cut(s) | 132 |
| MspI | CCGG | 9 cut(s) | 36, 95, 168, 174, 240, 282, 367, 390, 435 |
| Mva1269I | GAATGC | 1 cut(s) | 991 |
| MvaI | CCWGG | 8 cut(s) | 32, 44, 356, 398, 407, 527, 650, 1092 |
| MvnI | CGCG | 4 cut(s) | 132, 190, 192, 315 |
| MwoI | GCNNNNNNNGC | 7 cut(s) | 187, 466, 586, 839, 995, 1205, 1316 |
| NaeI | GCCGGC | 1 cut(s) | 175 |
| NarI | GGCGCC | 1 cut(s) | 171 |
| NciI | CCSGG | 2 cut(s) | 391, 436 |
| NcoI | CCATGG | 1 cut(s) | 925 |
| NgoMIV | GCCGGC | 1 cut(s) | 173 |
| NlaIII | CATG | 7 cut(s) | 86, 805, 904, 929, 940, 1132, 1240 |
| NmeAIII | GCCGAG | 2 cut(s) | 135, 1336 |
| NmuCI | GTSAC | 2 cut(s) | 754, 1097 |
| OliI | CACNNNNGTG | 2 cut(s) | 596, 1148 |
| PaeR7I | CTCGAG | 2 cut(s) | 77, 209 |
| PauI | GCGCGC | 1 cut(s) | 190 |
| PciSI | GCTCTTC | 1 cut(s) | 263 |
| PcsI | WCGNNNNNNNCGW | 3 cut(s) | 132, 207, 216 |
| PctI | GAATGC | 1 cut(s) | 991 |
| PdiI | GCCGGC | 1 cut(s) | 175 |
| PfeI | GAWTC | 3 cut(s) | 39, 402, 504 |
| PflFI | GACNNNGTC | 1 cut(s) | 230 |
| PleI | GAGTC | 1 cut(s) | 1321 |
| PluTI | GGCGCC | 1 cut(s) | 174 |
| PpsI | GAGTC | 1 cut(s) | 1321 |
| Psp6I | CCWGG | 8 cut(s) | 30, 42, 354, 396, 405, 525, 648, 1090 |
| PspEI | GGTNACC | 1 cut(s) | 1097 |
| PspFI | CCCAGC | 1 cut(s) | 60 |
| PspGI | CCWGG | 8 cut(s) | 30, 42, 354, 396, 405, 525, 648, 1090 |
| PspPI | GGNCC | 2 cut(s) | 232, 1261 |
| PspXI | VCTCGAGB | 1 cut(s) | 209 |
| PstI | CTGCAG | 1 cut(s) | 835 |
| PstNI | CAGNNNCTG | 1 cut(s) | 866 |
| PsuI | RGATCY | 4 cut(s) | 87, 363, 792, 1270 |
| PsyI | GACNNNGTC | 1 cut(s) | 230 |
| PteI | GCGCGC | 1 cut(s) | 190 |
| RsaI | GTAC | 6 cut(s) | 433, 935, 956, 1018, 1196, 1352 |
| RsaNI | GTAC | 6 cut(s) | 432, 934, 955, 1017, 1195, 1351 |
| RseI | CAYNNNNRTG | 4 cut(s) | 594, 596, 1148, 1221 |
| SacII | CCGCGG | 1 cut(s) | 133 |
| SalI | GTCGAC | 1 cut(s) | 203 |
| SapI | GCTCTTC | 1 cut(s) | 263 |
| Sau96I | GGNCC | 2 cut(s) | 232, 1261 |
| ScaI | AGTACT | 1 cut(s) | 1352 |
| SchI | GAGTC | 1 cut(s) | 1322 |
| SduI | GDGCHC | 6 cut(s) | 216, 396, 567, 591, 742, 1043 |
| SfaNI | GCATC | 1 cut(s) | 96 |
| SfcI | CTRYAG | 1 cut(s) | 831 |
| SfoI | GGCGCC | 1 cut(s) | 172 |
| Sfr274I | CTCGAG | 2 cut(s) | 77, 209 |
| Sfr303I | CCGCGG | 1 cut(s) | 133 |
| SgrBI | CCGCGG | 1 cut(s) | 133 |
| SinI | GGWCC | 1 cut(s) | 232 |
| SlaI | CTCGAG | 2 cut(s) | 77, 209 |
| SmiMI | CAYNNNNRTG | 4 cut(s) | 594, 596, 1148, 1221 |
| SmlI | CTYRAG | 3 cut(s) | 77, 209, 584 |
| SmoI | CTYRAG | 3 cut(s) | 77, 209, 584 |
| SspDI | GGCGCC | 1 cut(s) | 170 |
| StyI | CCWWGG | 2 cut(s) | 788, 925 |
| TaaI | ACNGT | 7 cut(s) | 74, 124, 220, 511, 560, 881, 1084 |
| TaiI | ACGT | 1 cut(s) | 186 |
| TaqI | TCGA | 7 cut(s) | 17, 78, 204, 210, 225, 345, 612 |
| TatI | WGTACW | 2 cut(s) | 933, 1350 |
| TauI | GCSGC | 6 cut(s) | 132, 135, 299, 318, 642, 992 |
| TfiI | GAWTC | 3 cut(s) | 39, 402, 504 |
| TscAI | CASTG | 6 cut(s) | 514, 565, 867, 886, 1087, 1153 |
| TseFI | GTSAC | 2 cut(s) | 754, 1097 |
| TseI | GCWGC | 4 cut(s) | 271, 580, 853, 1277 |
| Tsp45I | GTSAC | 2 cut(s) | 754, 1097 |
| TspDTI | ATGAA | 3 cut(s) | 696, 790, 1353 |
| TspRI | CASTG | 6 cut(s) | 514, 565, 867, 886, 1087, 1153 |
| Tth111I | GACNNNGTC | 1 cut(s) | 230 |
| VpaK11BI | GGWCC | 1 cut(s) | 232 |
| XagI | CCTNNNNNAGG | 2 cut(s) | 724, 1106 |
| XcmI | CCANNNNNNNNNTGG | 2 cut(s) | 541, 1027 |
| XhoI | CTCGAG | 2 cut(s) | 77, 209 |
| XmiI | GTMKAC | 1 cut(s) | 204 |
| ZrmI | AGTACT | 1 cut(s) | 1352 |