Rmu_sc0004780.1_g000001

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0004780.1
OG0000549
Physical Location & Seq
Forward (+)
3018 .. 3822
805 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0004780.1_g000001.1.cds

Sequence Viewer

Length: 441 bp
atgctaagcgacgacgccttcaacaccttcttctccgagaccggggctggcaagcacgtcccacgccccatcttcgtcgacctcgagtccaccatcaaagcttctgaacaggtaatcccacccggagttggcgaagtcgttgaacaaacaatcaaagcttctgagatttgttcgattgttcctcatcaaaccatgacctctagcgttcctcaatccagaccgcgagtagccagacctgcatctagtcgccgatcttggaagactgaagttttctgggtgcctagagcaatcgtcggtgtcttcgtccccagtcaatctgatttggtggccatagcaatttctgggtctgatggccggattcccgtcactggtgtccacagccggcggccggtcactagagtcaggcgactggtcaccggagtctggcaaggtctcggatga

Protein Analysis

146

Amino Acids

15.84

Weight (kDa)

10.35

Isoelectric Point (pI)

56.32

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000549)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G04820 AT1G50010 AT4G14960 AT4G14960
fragaria_vesca FvH4_1g18660 FvH4_2g03140 FvH4_5g33560 FvH4_6g48940 FvH4_6g48940
malus_domestica MD02G1204800.v1.1 MD05G1106800.v1.1 MD08G1210600.v1.1 MD09G1049500.v1.1 MD10G1111600.v1.1 MD15G1398700.v1.1 MD17G1049700.v1.1
prunus_persica Prupe.1G542600_v2.0.a1 Prupe.3G269300_v2.0.a1 Prupe.6G212700_v2.0.a1 Prupe.8G150900_v2.0.a1
pyrus_communis pycom05g10370 pycom08g18150 pycom10g09640 pycom10g09660 pycom111g04050 pycom15g35470 pycom17g04460
rosa_chinensis RchiOBHm_Chr2g0109481 RchiOBHm_Chr2g0168751 RchiOBHm_Chr3g0453071 RchiOBHm_Chr3g0460741 RchiOBHm_Chr3g0467971 RchiOBHm_Chr4g0400901 RchiOBHm_Chr6g0248011 RchiOBHm_Chr7g0232381 RchiOBHm_Chr7g0238121
rosa_laevigata RLG00000001350 RLG00000009167 RLG00000015163 RLG00000017708 RLG00000017741 RLG00000021807 RLG00000023849 RLG00000026518
rosa_multiflora Rmu_sc0000332.1_g000021 Rmu_sc0000332.1_g000029 Rmu_sc0002416.1_g000007 Rmu_sc0002611.1_g000001 Rmu_sc0003113.1_g000016 Rmu_sc0004780.1_g000001 Rmu_sc0004805.1_g000035 Rmu_sc0010633.1_g000010 Rmu_sc0011069.1_g000003 Rmu_sc0016170.1_g000002 Rmu_sc0025757.1_g000003 Rmu_ssc0000484.1_g000003
rosa_roxburghii Rroxscaffold_2G00134200 Rroxscaffold_4G00321030 Rroxscaffold_7G00213500
rosa_rugosa Rorug01G0283100 Rorug02G0160500 Rorug02G0535300 Rorug04G0027000 Rorug04G0328100 Rorug05G0388700 Rorug05G0530200 Rorug06G0489200 Rorug07G0104900 Rorug07G0209400 Rorug07G0274500
rosa_samantha Rh2BG222800 Rh2BG618900 Rh2CG214400 Rh2CG587200 Rh6BG040200 Rh6CG206500 Rh6DG035600 Rh7BG401600 Rh7BG401700 Rh7DG418400
rosa_wichuraiana Rw2G050240 Rw6G003920 Rw7G035430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 244
AccB1I GGYRCC 1 cut(s) 277
AccI GTMKAC 1 cut(s) 78
AccII CGCG 1 cut(s) 223
AciI CCGC 2 cut(s) 221, 385
AcoI YGGCCR 3 cut(s) 327, 352, 386
AcuI CTGAAG 1 cut(s) 285
AcyI GRCGYC 1 cut(s) 15
AfiI CCNNNNNNNGG 5 cut(s) 42, 128, 368, 388, 423
AgsI TTSAA 2 cut(s) 22, 143
AhdI GACNNNNNGTC 1 cut(s) 85
AjiI CACGTC 1 cut(s) 58
AloI GAACNNNNNNTCC 2 cut(s) 99, 131
AluBI AGCT 2 cut(s) 101, 158
AluI AGCT 2 cut(s) 101, 158
Alw26I GTCTC 2 cut(s) 32, 437
Ama87I CYCGRG 1 cut(s) 83
AoxI GGCC 3 cut(s) 327, 352, 386
AsuC2I CCSGG 2 cut(s) 43, 123
AsuHPI GGTGA 1 cut(s) 406
AvaI CYCGRG 1 cut(s) 83
BalI TGGCCA 1 cut(s) 329
BanI GGYRCC 1 cut(s) 277
BbsI GAAGAC 2 cut(s) 266, 292
BccI CCATC 3 cut(s) 77, 101, 344
BcnI CCSGG 2 cut(s) 43, 123
BcoDI GTCTC 2 cut(s) 32, 437
BfaI CTAG 4 cut(s) 201, 243, 282, 396
BfuAI ACCTGC 1 cut(s) 244
BisI GCNGC 1 cut(s) 386
BlpI GCTNAGC 1 cut(s) 5
BlsI GCNGC 1 cut(s) 387
Bme1390I CCNGG 2 cut(s) 43, 123
BmeRI GACNNNNNGTC 1 cut(s) 85
BmeT110I CYCGRG 1 cut(s) 83
BmgBI CACGTC 1 cut(s) 58
BmiI GGNNCC 1 cut(s) 279
BmrFI CCNGG 2 cut(s) 43, 123
BmrI ACTGGG 1 cut(s) 303
BmsI GCATC 1 cut(s) 248
BmuI ACTGGG 1 cut(s) 303
BpiI GAAGAC 2 cut(s) 266, 292
Bpu1102I GCTNAGC 1 cut(s) 5
BpuMI CCSGG 2 cut(s) 43, 123
BsaHI GRCGYC 1 cut(s) 15
BsaI GGTCTC 2 cut(s) 32, 437
BsaJI CCNNGG 1 cut(s) 42
BsaWI WCCGGW 1 cut(s) 416
Bsc4I CCNNNNNNNGG 5 cut(s) 42, 128, 368, 388, 423
Bse118I RCCGGY 2 cut(s) 381, 388
Bse1I ACTGG 3 cut(s) 309, 373, 414
BseDI CCNNGG 1 cut(s) 42
BseLI CCNNNNNNNGG 5 cut(s) 42, 128, 368, 388, 423
BseMII CTCAG 1 cut(s) 153
BseNI ACTGG 3 cut(s) 309, 373, 414
BseX3I CGGCCG 1 cut(s) 386
Bsh1236I CGCG 1 cut(s) 223
Bsh1285I CGRYCG 1 cut(s) 389
BshFI GGCC 3 cut(s) 329, 354, 388
BshNI GGYRCC 1 cut(s) 277
BsiEI CGRYCG 1 cut(s) 389
BsiHKCI CYCGRG 1 cut(s) 83
BsiSI CCGG 6 cut(s) 42, 123, 355, 382, 389, 417
BslFI GGGAC 2 cut(s) 44, 290
BslI CCNNNNNNNGG 5 cut(s) 42, 128, 368, 388, 423
BsmAI GTCTC 2 cut(s) 32, 437
BsmFI GGGAC 2 cut(s) 44, 290
BsnI GGCC 3 cut(s) 329, 354, 388
Bso31I GGTCTC 2 cut(s) 32, 437
BsoBI CYCGRG 1 cut(s) 83
Bsp143I GATC 1 cut(s) 251
Bsp1720I GCTNAGC 1 cut(s) 5
BspACI CCGC 2 cut(s) 221, 385
BspANI GGCC 3 cut(s) 329, 354, 388
BspCNI CTCAG 1 cut(s) 154
BspFNI CGCG 1 cut(s) 223
BspLI GGNNCC 1 cut(s) 279
BspMI ACCTGC 1 cut(s) 244
BspT107I GGYRCC 1 cut(s) 277
BspTNI GGTCTC 2 cut(s) 32, 437
BsrFI RCCGGY 2 cut(s) 381, 388
BsrI ACTGG 3 cut(s) 309, 373, 414
BssAI RCCGGY 2 cut(s) 381, 388
BssECI CCNNGG 1 cut(s) 42
BssMI GATC 1 cut(s) 251
BssNI GRCGYC 1 cut(s) 15
BstACI GRCGYC 1 cut(s) 15
BstC8I GCNNGC 3 cut(s) 49, 53, 383
BstDEI CTNAG 2 cut(s) 5, 162
BstEII GGTNACC 1 cut(s) 412
BstFNI CGCG 1 cut(s) 223
BstKTI GATC 1 cut(s) 254
BstMAI GTCTC 2 cut(s) 32, 437
BstMBI GATC 1 cut(s) 251
BstMCI CGRYCG 1 cut(s) 389
BstMWI GCNNNNNNNGC 1 cut(s) 236
BstPI GGTNACC 1 cut(s) 412
BstSCI CCNGG 2 cut(s) 41, 121
BstUI CGCG 1 cut(s) 223
BstV2I GAAGAC 2 cut(s) 266, 292
BstZI CGGCCG 1 cut(s) 386
BsuRI GGCC 3 cut(s) 329, 354, 388
BtrI CACGTC 1 cut(s) 58
BtsIMutI CAGTG 1 cut(s) 366
BveI ACCTGC 1 cut(s) 244
Cac8I GCNNGC 3 cut(s) 49, 53, 383
Cfr10I RCCGGY 2 cut(s) 381, 388
CseI GACGC 1 cut(s) 23
CviAII CATG 1 cut(s) 193
CviJI RGCY 8 cut(s) 47, 101, 158, 230, 329, 354, 381, 388
CviKI_1 RGCY 8 cut(s) 47, 101, 158, 230, 329, 354, 381, 388
DdeI CTNAG 2 cut(s) 5, 162
DpnI GATC 1 cut(s) 253
DpnII GATC 1 cut(s) 251
DriI GACNNNNNGTC 1 cut(s) 85
EaeI YGGCCR 3 cut(s) 327, 352, 386
EagI CGGCCG 1 cut(s) 386
Eam1105I GACNNNNNGTC 1 cut(s) 85
EclXI CGGCCG 1 cut(s) 386
Eco31I GGTCTC 2 cut(s) 32, 437
Eco52I CGGCCG 1 cut(s) 386
Eco57I CTGAAG 1 cut(s) 285
Eco88I CYCGRG 1 cut(s) 83
Eco91I GGTNACC 1 cut(s) 412
EcoO65I GGTNACC 1 cut(s) 412
FaeI CATG 1 cut(s) 196
FaiI YATR 2 cut(s) 194, 332
FaqI GGGAC 2 cut(s) 44, 290
FatI CATG 1 cut(s) 192
FblI GTMKAC 1 cut(s) 78
Fnu4HI GCNGC 1 cut(s) 386
Fsp4HI GCNGC 1 cut(s) 386
FspBI CTAG 4 cut(s) 201, 243, 282, 396
GluI GCNGC 1 cut(s) 386
HaeIII GGCC 3 cut(s) 329, 354, 388
HapII CCGG 6 cut(s) 42, 123, 355, 382, 389, 417
HgaI GACGC 1 cut(s) 23
Hin1I GRCGYC 1 cut(s) 15
Hin1II CATG 1 cut(s) 196
HincII GTYRAC 1 cut(s) 79
HindII GTYRAC 1 cut(s) 79
HindIII AAGCTT 2 cut(s) 99, 156
HinfI GANTC 4 cut(s) 86, 358, 399, 420
HpaII CCGG 6 cut(s) 42, 123, 355, 382, 389, 417
HphI GGTGA 1 cut(s) 406
Hpy166II GTNNAC 3 cut(s) 79, 90, 376
Hpy188I TCNGA 6 cut(s) 37, 106, 163, 319, 349, 437
Hpy188III TCNNGA 1 cut(s) 216
Hpy8I GTNNAC 3 cut(s) 79, 90, 376
Hpy99I CGWCG 4 cut(s) 14, 17, 80, 296
HpyAV CCTTC 2 cut(s) 28, 37
HpyCH4IV ACGT 1 cut(s) 57
HpyCH4V TGCA 1 cut(s) 239
HpyF10VI GCNNNNNNNGC 1 cut(s) 236
HpyF3I CTNAG 2 cut(s) 5, 162
HpySE526I ACGT 1 cut(s) 57
Hsp92I GRCGYC 1 cut(s) 15
Hsp92II CATG 1 cut(s) 196
KroI GCCGGC 1 cut(s) 381
KroNI GCCGGC 1 cut(s) 383
Kzo9I GATC 1 cut(s) 251
LweI GCATC 1 cut(s) 248
MaeI CTAG 4 cut(s) 201, 243, 282, 396
MaeII ACGT 1 cut(s) 57
MaeIII GTNAC 3 cut(s) 364, 391, 412
MalI GATC 1 cut(s) 253
MboI GATC 1 cut(s) 251
MboII GAAGA 4 cut(s) 22, 64, 271, 292
MlsI TGGCCA 1 cut(s) 329
MluCI AATT 1 cut(s) 336
MluNI TGGCCA 1 cut(s) 329
MlyI GAGTC 3 cut(s) 95, 408, 429
MnlI CCTC 4 cut(s) 92, 192, 208, 219
Mox20I TGGCCA 1 cut(s) 329
MroNI GCCGGC 1 cut(s) 381
MscI TGGCCA 1 cut(s) 329
Msp20I TGGCCA 1 cut(s) 329
MspI CCGG 6 cut(s) 42, 123, 355, 382, 389, 417
MspR9I CCNGG 2 cut(s) 43, 123
MvnI CGCG 1 cut(s) 223
MwoI GCNNNNNNNGC 1 cut(s) 236
NaeI GCCGGC 1 cut(s) 383
NciI CCSGG 2 cut(s) 43, 123
NdeII GATC 1 cut(s) 251
NgoMIV GCCGGC 1 cut(s) 381
NlaIII CATG 1 cut(s) 196
NlaIV GGNNCC 1 cut(s) 279
NmuCI GTSAC 3 cut(s) 364, 391, 412
PaeR7I CTCGAG 1 cut(s) 83
PcsI WCGNNNNNNNCGW 2 cut(s) 81, 300
PdiI GCCGGC 1 cut(s) 383
PfeI GAWTC 1 cut(s) 358
PkrI GCNGC 1 cut(s) 387
PleI GAGTC 3 cut(s) 94, 407, 428
PpsI GAGTC 3 cut(s) 94, 407, 428
PspEI GGTNACC 1 cut(s) 412
PspN4I GGNNCC 1 cut(s) 279
PspXI VCTCGAGB 1 cut(s) 83
SalI GTCGAC 1 cut(s) 77
SatI GCNGC 1 cut(s) 386
Sau3AI GATC 1 cut(s) 251
SchI GAGTC 3 cut(s) 95, 408, 429
ScrFI CCNGG 2 cut(s) 43, 123
SetI ASST 9 cut(s) 29, 60, 84, 103, 114, 160, 200, 238, 433
SfaNI GCATC 1 cut(s) 248
Sfr274I CTCGAG 1 cut(s) 83
SlaI CTCGAG 1 cut(s) 83
SmlI CTYRAG 1 cut(s) 83
SmoI CTYRAG 1 cut(s) 83
Sse9I AATT 1 cut(s) 336
SsiI CCGC 2 cut(s) 221, 385
SspMI CTAG 4 cut(s) 201, 243, 282, 396
StyD4I CCNGG 2 cut(s) 41, 121
TaiI ACGT 1 cut(s) 60
TaqI TCGA 3 cut(s) 78, 84, 173
TasI AATT 1 cut(s) 336
TauI GCSGC 1 cut(s) 388
TfiI GAWTC 1 cut(s) 358
TscAI CASTG 1 cut(s) 373
TseFI GTSAC 3 cut(s) 364, 391, 412
Tsp45I GTSAC 3 cut(s) 364, 391, 412
TspRI CASTG 1 cut(s) 373
XhoI CTCGAG 1 cut(s) 83
XmiI GTMKAC 1 cut(s) 78
XspI CTAG 4 cut(s) 201, 243, 282, 396
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.