Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 1 cut(s) | 122 |
| Acc36I | ACCTGC | 1 cut(s) | 633 |
| Acc65I | GGTACC | 1 cut(s) | 431 |
| AccB1I | GGYRCC | 5 cut(s) | 170, 242, 431, 1038, 1307 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 1030 |
| AccBSI | CCGCTC | 1 cut(s) | 466 |
| AccII | CGCG | 2 cut(s) | 132, 192 |
| AclWI | GGATC | 7 cut(s) | 354, 445, 457, 787, 800, 911, 1278 |
| AcoI | YGGCCR | 4 cut(s) | 100, 127, 906, 996 |
| AcyI | GRCGYC | 1 cut(s) | 141 |
| AfaI | GTAC | 7 cut(s) | 433, 515, 845, 935, 956, 1196, 1223 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 926, 1030, 1108 |
| AflIII | ACRYGT | 1 cut(s) | 597 |
| AgeI | ACCGGT | 1 cut(s) | 167 |
| AgsI | TTSAA | 4 cut(s) | 148, 398, 415, 1054 |
| AjiI | CACGTC | 2 cut(s) | 184, 598 |
| AjnI | CCWGG | 2 cut(s) | 354, 788 |
| AjuI | GAANNNNNNNTTGG | 4 cut(s) | 488, 520, 1193, 1225 |
| AleI | CACNNNNGTG | 1 cut(s) | 596 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 1235, 1267 |
| AluBI | AGCT | 7 cut(s) | 67, 256, 274, 373, 499, 740, 856 |
| AluI | AGCT | 7 cut(s) | 67, 256, 274, 373, 499, 740, 856 |
| Alw21I | GWGCWC | 4 cut(s) | 69, 84, 591, 742 |
| Alw26I | GTCTC | 2 cut(s) | 158, 697 |
| AlwI | GGATC | 7 cut(s) | 354, 445, 457, 787, 800, 911, 1278 |
| Ama87I | CYCGRG | 1 cut(s) | 77 |
| AoxI | GGCC | 7 cut(s) | 91, 100, 127, 621, 906, 939, 996 |
| ApeKI | GCWGC | 5 cut(s) | 271, 296, 853, 945, 1277 |
| AsiGI | ACCGGT | 1 cut(s) | 167 |
| Asp718I | GGTACC | 1 cut(s) | 431 |
| AspLEI | GCGC | 1 cut(s) | 194 |
| AspS9I | GGNCC | 4 cut(s) | 92, 232, 358, 645 |
| AsuC2I | CCSGG | 2 cut(s) | 1048, 1092 |
| AsuHPI | GGTGA | 6 cut(s) | 865, 974, 1109, 1241, 1346, 1355 |
| AvaI | CYCGRG | 1 cut(s) | 77 |
| AvaII | GGWCC | 3 cut(s) | 232, 358, 645 |
| AxyI | CCTNAGG | 1 cut(s) | 525 |
| BaeGI | GKGCMC | 1 cut(s) | 1043 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 1213, 1246 |
| BalI | TGGCCA | 3 cut(s) | 102, 908, 998 |
| BamHI | GGATCC | 1 cut(s) | 792 |
| BanI | GGYRCC | 5 cut(s) | 170, 242, 431, 1038, 1307 |
| BanII | GRGCYC | 3 cut(s) | 69, 216, 742 |
| BauI | CACGAG | 2 cut(s) | 848, 1263 |
| BbsI | GAAGAC | 2 cut(s) | 297, 403 |
| Bbv12I | GWGCWC | 4 cut(s) | 69, 84, 591, 742 |
| BbvCI | CCTCAGC | 1 cut(s) | 1155 |
| BbvI | GCAGC | 5 cut(s) | 283, 283, 865, 932, 1264 |
| BccI | CCATC | 6 cut(s) | 92, 203, 335, 632, 898, 1010 |
| BceAI | ACGGC | 2 cut(s) | 100, 404 |
| BcgI | CGANNNNNNTGC | 6 cut(s) | 152, 186, 322, 356, 631, 665 |
| BciT130I | CCWGG | 2 cut(s) | 356, 790 |
| BclI | TGATCA | 1 cut(s) | 1174 |
| BcnI | CCSGG | 2 cut(s) | 1048, 1092 |
| BcoDI | GTCTC | 2 cut(s) | 158, 697 |
| BfuAI | ACCTGC | 1 cut(s) | 633 |
| BglI | GCCNNNNNGGC | 2 cut(s) | 99, 995 |
| Bme1390I | CCNGG | 4 cut(s) | 356, 790, 1048, 1092 |
| Bme18I | GGWCC | 3 cut(s) | 232, 358, 645 |
| BmeT110I | CYCGRG | 1 cut(s) | 77 |
| BmgBI | CACGTC | 2 cut(s) | 184, 598 |
| BmgT120I | GGNCC | 4 cut(s) | 92, 232, 358, 645 |
| BmrFI | CCNGG | 4 cut(s) | 356, 790, 1048, 1092 |
| BmrI | ACTGGG | 1 cut(s) | 399 |
| BmsI | GCATC | 2 cut(s) | 96, 372 |
| BmuI | ACTGGG | 1 cut(s) | 399 |
| BpiI | GAAGAC | 2 cut(s) | 297, 403 |
| BplI | GAGNNNNNCTC | 6 cut(s) | 842, 874, 881, 913, 1148, 1180 |
| BpmI | CTGGAG | 4 cut(s) | 230, 482, 605, 1304 |
| Bpu10I | CCTNAGC | 2 cut(s) | 267, 1155 |
| BpuMI | CCSGG | 2 cut(s) | 1048, 1092 |
| BsaAI | YACGTR | 1 cut(s) | 1225 |
| BsaHI | GRCGYC | 1 cut(s) | 141 |
| BsaI | GGTCTC | 1 cut(s) | 158 |
| BsaJI | CCNNGG | 8 cut(s) | 130, 313, 788, 925, 958, 975, 1107, 1320 |
| BsaWI | WCCGGW | 2 cut(s) | 167, 434 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 926, 1030, 1108 |
| Bse118I | RCCGGY | 2 cut(s) | 167, 239 |
| Bse1I | ACTGG | 3 cut(s) | 394, 1024, 1146 |
| Bse21I | CCTNAGG | 1 cut(s) | 525 |
| Bse3DI | GCAATG | 1 cut(s) | 452 |
| BseBI | CCWGG | 2 cut(s) | 356, 790 |
| BseDI | CCNNGG | 8 cut(s) | 130, 313, 788, 925, 958, 975, 1107, 1320 |
| BseGI | GGATG | 2 cut(s) | 757, 985 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 926, 1030, 1108 |
| BseMI | GCAATG | 1 cut(s) | 452 |
| BseMII | CTCAG | 7 cut(s) | 258, 539, 710, 825, 858, 1146, 1269 |
| BseNI | ACTGG | 3 cut(s) | 394, 1024, 1146 |
| BseRI | GAGGAG | 2 cut(s) | 513, 1313 |
| BseSI | GKGCMC | 1 cut(s) | 1043 |
| BseX3I | CGGCCG | 1 cut(s) | 127 |
| BseXI | GCAGC | 5 cut(s) | 283, 283, 865, 932, 1264 |
| BseYI | CCCAGC | 3 cut(s) | 60, 110, 499 |
| BsgI | GTGCAG | 2 cut(s) | 370, 1133 |
| Bsh1236I | CGCG | 2 cut(s) | 132, 192 |
| Bsh1285I | CGRYCG | 1 cut(s) | 130 |
| BshFI | GGCC | 7 cut(s) | 93, 102, 129, 623, 908, 941, 998 |
| BshNI | GGYRCC | 5 cut(s) | 170, 242, 431, 1038, 1307 |
| BshTI | ACCGGT | 1 cut(s) | 167 |
| BsiEI | CGRYCG | 1 cut(s) | 130 |
| BsiHKAI | GWGCWC | 4 cut(s) | 69, 84, 591, 742 |
| BsiHKCI | CYCGRG | 1 cut(s) | 77 |
| BsiSI | CCGG | 8 cut(s) | 36, 168, 174, 240, 282, 435, 1047, 1092 |
| BslFI | GGGAC | 3 cut(s) | 170, 631, 648 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 926, 1030, 1108 |
| BsmAI | GTCTC | 2 cut(s) | 158, 697 |
| BsmBI | CGTCTC | 1 cut(s) | 697 |
| BsmFI | GGGAC | 3 cut(s) | 170, 631, 648 |
| BsmI | GAATGC | 1 cut(s) | 991 |
| BsnI | GGCC | 7 cut(s) | 93, 102, 129, 623, 908, 941, 998 |
| Bso31I | GGTCTC | 1 cut(s) | 158 |
| BsoBI | CYCGRG | 1 cut(s) | 77 |
| Bsp1286I | GDGCHC | 6 cut(s) | 69, 84, 216, 591, 742, 1043 |
| Bsp19I | CCATGG | 1 cut(s) | 925 |
| BspANI | GGCC | 7 cut(s) | 93, 102, 129, 623, 908, 941, 998 |
| BspCNI | CTCAG | 7 cut(s) | 259, 538, 709, 826, 859, 1147, 1268 |
| BspFNI | CGCG | 2 cut(s) | 132, 192 |
| BspMI | ACCTGC | 1 cut(s) | 633 |
| BspPI | GGATC | 7 cut(s) | 354, 445, 457, 787, 800, 911, 1278 |
| BspQI | GCTCTTC | 1 cut(s) | 263 |
| BspT107I | GGYRCC | 5 cut(s) | 170, 242, 431, 1038, 1307 |
| BspTNI | GGTCTC | 1 cut(s) | 158 |
| BsrBI | CCGCTC | 1 cut(s) | 466 |
| BsrDI | GCAATG | 1 cut(s) | 452 |
| BsrFI | RCCGGY | 2 cut(s) | 167, 239 |
| BsrI | ACTGG | 3 cut(s) | 394, 1024, 1146 |
| BssAI | RCCGGY | 2 cut(s) | 167, 239 |
| BssECI | CCNNGG | 8 cut(s) | 130, 313, 788, 925, 958, 975, 1107, 1320 |
| BssNI | GRCGYC | 1 cut(s) | 141 |
| BssSI | CACGAG | 2 cut(s) | 848, 1263 |
| BssT1I | CCWWGG | 3 cut(s) | 925, 975, 1107 |
| Bst2BI | CACGAG | 2 cut(s) | 848, 1263 |
| Bst2UI | CCWGG | 2 cut(s) | 356, 790 |
| Bst4CI | ACNGT | 8 cut(s) | 124, 220, 511, 560, 881, 959, 1084, 1334 |
| Bst6I | CTCTTC | 1 cut(s) | 263 |
| BstACI | GRCGYC | 1 cut(s) | 141 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 57 |
| BstBAI | YACGTR | 1 cut(s) | 1225 |
| BstC8I | GCNNGC | 7 cut(s) | 33, 58, 254, 375, 894, 943, 1206 |
| BstDEI | CTNAG | 7 cut(s) | 267, 525, 696, 834, 867, 1155, 1255 |
| BstDSI | CCRYGG | 4 cut(s) | 130, 313, 925, 958 |
| BstEII | GGTNACC | 1 cut(s) | 1097 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 1106 |
| BstF5I | GGATG | 2 cut(s) | 757, 985 |
| BstFNI | CGCG | 2 cut(s) | 132, 192 |
| BstHHI | GCGC | 1 cut(s) | 194 |
| BstMAI | GTCTC | 2 cut(s) | 158, 697 |
| BstMCI | CGRYCG | 1 cut(s) | 130 |
| BstNI | CCWGG | 2 cut(s) | 356, 790 |
| BstPI | GGTNACC | 1 cut(s) | 1097 |
| BstSCI | CCNGG | 4 cut(s) | 354, 788, 1046, 1090 |
| BstSLI | GKGCMC | 1 cut(s) | 1043 |
| BstUI | CGCG | 2 cut(s) | 132, 192 |
| BstV1I | GCAGC | 5 cut(s) | 283, 283, 865, 932, 1264 |
| BstV2I | GAAGAC | 2 cut(s) | 297, 403 |
| BstX2I | RGATCY | 3 cut(s) | 346, 792, 1270 |
| BstYI | RGATCY | 3 cut(s) | 346, 792, 1270 |
| BstZI | CGGCCG | 1 cut(s) | 127 |
| Bsu36I | CCTNAGG | 1 cut(s) | 525 |
| BsuRI | GGCC | 7 cut(s) | 93, 102, 129, 623, 908, 941, 998 |
| BtgI | CCRYGG | 4 cut(s) | 130, 313, 925, 958 |
| BtgZI | GCGATG | 1 cut(s) | 150 |
| BtrI | CACGTC | 2 cut(s) | 184, 598 |
| BtsCI | GGATG | 2 cut(s) | 757, 985 |
| BtsIMutI | CAGTG | 5 cut(s) | 387, 507, 565, 886, 1153 |
| BveI | ACCTGC | 1 cut(s) | 633 |
| Cac8I | GCNNGC | 7 cut(s) | 33, 58, 254, 375, 894, 943, 1206 |
| CfoI | GCGC | 1 cut(s) | 194 |
| Cfr10I | RCCGGY | 2 cut(s) | 167, 239 |
| Cfr13I | GGNCC | 4 cut(s) | 92, 232, 358, 645 |
| Cfr42I | CCGCGG | 1 cut(s) | 133 |
| CseI | GACGC | 4 cut(s) | 149, 302, 650, 708 |
| Csp6I | GTAC | 7 cut(s) | 432, 514, 844, 934, 955, 1195, 1222 |
| CspAI | ACCGGT | 1 cut(s) | 167 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 1132, 1167 |
| CviAII | CATG | 5 cut(s) | 901, 926, 937, 1129, 1237 |
| CviQI | GTAC | 7 cut(s) | 432, 514, 844, 934, 955, 1195, 1222 |
| DdeI | CTNAG | 7 cut(s) | 267, 525, 696, 834, 867, 1155, 1255 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 122 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 122 |
| EaeI | YGGCCR | 4 cut(s) | 100, 127, 906, 996 |
| EagI | CGGCCG | 1 cut(s) | 127 |
| Eam1104I | CTCTTC | 1 cut(s) | 263 |
| EarI | CTCTTC | 1 cut(s) | 263 |
| Ecl136II | GAGCTC | 2 cut(s) | 67, 740 |
| EclXI | CGGCCG | 1 cut(s) | 127 |
| Eco130I | CCWWGG | 3 cut(s) | 925, 975, 1107 |
| Eco24I | GRGCYC | 3 cut(s) | 69, 216, 742 |
| Eco31I | GGTCTC | 1 cut(s) | 158 |
| Eco47I | GGWCC | 3 cut(s) | 232, 358, 645 |
| Eco52I | CGGCCG | 1 cut(s) | 127 |
| Eco53kI | GAGCTC | 2 cut(s) | 67, 740 |
| Eco81I | CCTNAGG | 1 cut(s) | 525 |
| Eco88I | CYCGRG | 1 cut(s) | 77 |
| Eco91I | GGTNACC | 1 cut(s) | 1097 |
| EcoICRI | GAGCTC | 2 cut(s) | 67, 740 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 1106 |
| EcoO109I | RGGNCCY | 1 cut(s) | 645 |
| EcoO65I | GGTNACC | 1 cut(s) | 1097 |
| EcoRII | CCWGG | 2 cut(s) | 354, 788 |
| EcoT14I | CCWWGG | 3 cut(s) | 925, 975, 1107 |
| EcoT38I | GRGCYC | 3 cut(s) | 69, 216, 742 |
| ErhI | CCWWGG | 3 cut(s) | 925, 975, 1107 |
| Esp3I | CGTCTC | 1 cut(s) | 697 |
| FaeI | CATG | 5 cut(s) | 904, 929, 940, 1132, 1240 |
| FaiI | YATR | 9 cut(s) | 784, 803, 816, 902, 927, 938, 1130, 1238, 1296 |
| FaqI | GGGAC | 3 cut(s) | 170, 631, 648 |
| FatI | CATG | 5 cut(s) | 900, 925, 936, 1128, 1236 |
| FauI | CCCGC | 2 cut(s) | 197, 929 |
| FbaI | TGATCA | 1 cut(s) | 1174 |
| FokI | GGATG | 2 cut(s) | 764, 992 |
| FriOI | GRGCYC | 3 cut(s) | 69, 216, 742 |
| GlaI | GCGC | 1 cut(s) | 193 |
| GsaI | CCCAGC | 3 cut(s) | 64, 114, 503 |
| GsuI | CTGGAG | 4 cut(s) | 230, 482, 605, 1304 |
| HaeIII | GGCC | 7 cut(s) | 93, 102, 129, 623, 908, 941, 998 |
| HapII | CCGG | 8 cut(s) | 36, 168, 174, 240, 282, 435, 1047, 1092 |
| HgaI | GACGC | 4 cut(s) | 149, 302, 650, 708 |
| HhaI | GCGC | 1 cut(s) | 194 |
| Hin1I | GRCGYC | 1 cut(s) | 141 |
| Hin1II | CATG | 5 cut(s) | 904, 929, 940, 1132, 1240 |
| Hin6I | GCGC | 1 cut(s) | 192 |
| HinP1I | GCGC | 1 cut(s) | 192 |
| HinfI | GANTC | 4 cut(s) | 39, 504, 682, 1313 |
| HpaII | CCGG | 8 cut(s) | 36, 168, 174, 240, 282, 435, 1047, 1092 |
| HphI | GGTGA | 6 cut(s) | 865, 974, 1109, 1241, 1346, 1355 |
| Hpy166II | GTNNAC | 1 cut(s) | 514 |
| Hpy188I | TCNGA | 5 cut(s) | 163, 681, 744, 1191, 1258 |
| Hpy188III | TCNNGA | 7 cut(s) | 209, 367, 461, 766, 1006, 1265, 1283 |
| Hpy8I | GTNNAC | 1 cut(s) | 514 |
| Hpy99I | CGWCG | 7 cut(s) | 128, 206, 230, 425, 599, 724, 737 |
| HpyAV | CCTTC | 6 cut(s) | 154, 163, 528, 832, 1238, 1282 |
| HpyCH4III | ACNGT | 8 cut(s) | 124, 220, 511, 560, 881, 959, 1084, 1334 |
| HpyCH4IV | ACGT | 3 cut(s) | 183, 597, 1224 |
| HpyCH4V | TGCA | 5 cut(s) | 12, 387, 1114, 1128, 1216 |
| HpyF3I | CTNAG | 7 cut(s) | 267, 525, 696, 834, 867, 1155, 1255 |
| HpySE526I | ACGT | 3 cut(s) | 183, 597, 1224 |
| Hsp92I | GRCGYC | 1 cut(s) | 141 |
| Hsp92II | CATG | 5 cut(s) | 904, 929, 940, 1132, 1240 |
| HspAI | GCGC | 1 cut(s) | 192 |
| KpnI | GGTACC | 1 cut(s) | 435 |
| Ksp22I | TGATCA | 1 cut(s) | 1174 |
| KspI | CCGCGG | 1 cut(s) | 133 |
| LguI | GCTCTTC | 1 cut(s) | 263 |
| LmnI | GCTCC | 7 cut(s) | 64, 211, 463, 547, 586, 737, 823 |
| Lsp1109I | GCAGC | 5 cut(s) | 283, 283, 865, 932, 1264 |
| LweI | GCATC | 2 cut(s) | 96, 372 |
| MaeII | ACGT | 3 cut(s) | 183, 597, 1224 |
| MaeIII | GTNAC | 4 cut(s) | 317, 754, 1097, 1334 |
| MbiI | CCGCTC | 1 cut(s) | 466 |
| MboII | GAAGA | 6 cut(s) | 148, 190, 250, 302, 403, 699 |
| MflI | RGATCY | 3 cut(s) | 346, 792, 1270 |
| MhlI | GDGCHC | 6 cut(s) | 69, 84, 216, 591, 742, 1043 |
| MlsI | TGGCCA | 3 cut(s) | 102, 908, 998 |
| MluCI | AATT | 1 cut(s) | 1066 |
| MluNI | TGGCCA | 3 cut(s) | 102, 908, 998 |
| MlyI | GAGTC | 1 cut(s) | 1322 |
| MmeI | TCCRAC | 1 cut(s) | 1161 |
| Mox20I | TGGCCA | 3 cut(s) | 102, 908, 998 |
| MscI | TGGCCA | 3 cut(s) | 102, 908, 998 |
| MslI | CAYNNNNRTG | 1 cut(s) | 596 |
| Msp20I | TGGCCA | 3 cut(s) | 102, 908, 998 |
| MspA1I | CMGCKG | 3 cut(s) | 132, 833, 992 |
| MspI | CCGG | 8 cut(s) | 36, 168, 174, 240, 282, 435, 1047, 1092 |
| MspR9I | CCNGG | 4 cut(s) | 356, 790, 1048, 1092 |
| Mva1269I | GAATGC | 1 cut(s) | 991 |
| MvaI | CCWGG | 2 cut(s) | 356, 790 |
| MvnI | CGCG | 2 cut(s) | 132, 192 |
| NciI | CCSGG | 2 cut(s) | 1048, 1092 |
| NcoI | CCATGG | 1 cut(s) | 925 |
| NlaIII | CATG | 5 cut(s) | 904, 929, 940, 1132, 1240 |
| NmeAIII | GCCGAG | 2 cut(s) | 1336, 1345 |
| NmuCI | GTSAC | 4 cut(s) | 317, 754, 1097, 1334 |
| OliI | CACNNNNGTG | 1 cut(s) | 596 |
| PaeR7I | CTCGAG | 1 cut(s) | 77 |
| PciSI | GCTCTTC | 1 cut(s) | 263 |
| PcsI | WCGNNNNNNNCGW | 2 cut(s) | 132, 716 |
| PctI | GAATGC | 1 cut(s) | 991 |
| PfeI | GAWTC | 3 cut(s) | 39, 504, 682 |
| PflFI | GACNNNGTC | 2 cut(s) | 230, 718 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 1030 |
| PinAI | ACCGGT | 1 cut(s) | 167 |
| PleI | GAGTC | 1 cut(s) | 1321 |
| PpsI | GAGTC | 1 cut(s) | 1321 |
| Ppu21I | YACGTR | 1 cut(s) | 1225 |
| PpuMI | RGGWCCY | 1 cut(s) | 645 |
| Psp124BI | GAGCTC | 2 cut(s) | 69, 742 |
| Psp5II | RGGWCCY | 1 cut(s) | 645 |
| Psp6I | CCWGG | 2 cut(s) | 354, 788 |
| PspEI | GGTNACC | 1 cut(s) | 1097 |
| PspFI | CCCAGC | 3 cut(s) | 60, 110, 499 |
| PspGI | CCWGG | 2 cut(s) | 354, 788 |
| PspPI | GGNCC | 4 cut(s) | 92, 232, 358, 645 |
| PspPPI | RGGWCCY | 1 cut(s) | 645 |
| PspXI | VCTCGAGB | 1 cut(s) | 77 |
| PsuI | RGATCY | 3 cut(s) | 346, 792, 1270 |
| PsyI | GACNNNGTC | 2 cut(s) | 230, 718 |
| RsaI | GTAC | 7 cut(s) | 433, 515, 845, 935, 956, 1196, 1223 |
| RsaNI | GTAC | 7 cut(s) | 432, 514, 844, 934, 955, 1195, 1222 |
| RseI | CAYNNNNRTG | 1 cut(s) | 596 |
| SacI | GAGCTC | 2 cut(s) | 69, 742 |
| SacII | CCGCGG | 1 cut(s) | 133 |
| SapI | GCTCTTC | 1 cut(s) | 263 |
| Sau96I | GGNCC | 4 cut(s) | 92, 232, 358, 645 |
| SchI | GAGTC | 1 cut(s) | 1322 |
| ScrFI | CCNGG | 4 cut(s) | 356, 790, 1048, 1092 |
| SduI | GDGCHC | 6 cut(s) | 69, 84, 216, 591, 742, 1043 |
| SfaNI | GCATC | 2 cut(s) | 96, 372 |
| SfiI | GGCCNNNNNGGCC | 1 cut(s) | 99 |
| Sfr274I | CTCGAG | 1 cut(s) | 77 |
| Sfr303I | CCGCGG | 1 cut(s) | 133 |
| SgrBI | CCGCGG | 1 cut(s) | 133 |
| SinI | GGWCC | 3 cut(s) | 232, 358, 645 |
| SlaI | CTCGAG | 1 cut(s) | 77 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 596 |
| SmlI | CTYRAG | 1 cut(s) | 77 |
| SmoI | CTYRAG | 1 cut(s) | 77 |
| Sse9I | AATT | 1 cut(s) | 1066 |
| SstI | GAGCTC | 2 cut(s) | 69, 742 |
| StyD4I | CCNGG | 4 cut(s) | 354, 788, 1046, 1090 |
| StyI | CCWWGG | 3 cut(s) | 925, 975, 1107 |
| TaaI | ACNGT | 8 cut(s) | 124, 220, 511, 560, 881, 959, 1084, 1334 |
| TaiI | ACGT | 3 cut(s) | 186, 600, 1227 |
| TaqI | TCGA | 7 cut(s) | 78, 204, 225, 612, 651, 657, 732 |
| TaqII | GACCGA | 1 cut(s) | 773 |
| TasI | AATT | 1 cut(s) | 1066 |
| TatI | WGTACW | 1 cut(s) | 933 |
| TauI | GCSGC | 5 cut(s) | 132, 135, 469, 642, 992 |
| TfiI | GAWTC | 3 cut(s) | 39, 504, 682 |
| TscAI | CASTG | 5 cut(s) | 394, 514, 565, 886, 1153 |
| TseFI | GTSAC | 4 cut(s) | 317, 754, 1097, 1334 |
| TseI | GCWGC | 5 cut(s) | 271, 296, 853, 945, 1277 |
| Tsp45I | GTSAC | 4 cut(s) | 317, 754, 1097, 1334 |
| TspGWI | ACGGA | 2 cut(s) | 498, 750 |
| TspRI | CASTG | 5 cut(s) | 394, 514, 565, 886, 1153 |
| Tth111I | GACNNNGTC | 2 cut(s) | 230, 718 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 1030 |
| VpaK11BI | GGWCC | 3 cut(s) | 232, 358, 645 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 1106 |
| XhoI | CTCGAG | 1 cut(s) | 77 |