pycom111g04050

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
SuperScaffold_111
OG0000549
Physical Location & Seq
Forward (+)
2468158 .. 2469850
1693 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom111g04050.4

Sequence Viewer

Length: 1353 bp
ATGAGAGAGTGCATCTCCATCCACATCGGCCAGGCCGGAATCCAAGTCGGAAACGCTTGCTGGGAGCTCTACTGCCTCGAACATGGCATCCAGCCTGATGGCCAAATGCCGAGTGACAAGACCGTCGGTGGCGGAGACGACGCCTTCAACACTTTCTTCAGCGAAACTGGAGCCGGAAAGCACGTCCCCCGTGCTGTGTTCTTGGATCTTGAGCCCACCGTCATCGATGAGGTCAGGACCGGCACTTACCGCCAGCTATTCCACCCGGAACAGCTCATCAGCGGCAAGGAAGACGCCGCTAATAACTTCGCCAGAGGCCACTACACAATTGGGAAGGAGATTGTGGATCTCTGCCTGGATCGAATCCGAAAGCTTGCCGACAACTGCACTGGTCTTCAAGGGTTCCTGGTGTTCCACGCTGTTGGAGGTGGGACTGGCTCTGGCCTCGGCTCTCTTCTCCTGGAAAGGCTATCTGTTGATTACGGAAAGAAATCGAAACTCGGGTTCACAGTCTACCCGTCTCCTCAGGTCTCAACCTCTGTCGTTGAGCCGTACAACAGCGTACTGTCCACTCACTCCCTCTTGGAGCACACTGATGTGTCGGTTCTCCTTGACAACGAAGCCATCTACGACATCTGCCGCAGGTCTCTAGACATTGAAAGACCCACTTACACCAACCTCAACAGGCTGGTCTCTCAGGTTATTTCCTCACTCACCGCTTCTCTGCGTTTTGATGGGGCGCTCAACGTGGACGTGACAGAGTTCCAGACCAATTTGGTCCCCTACCCAAGAATCCACTTCATGCTTTCGTCTTATGCACCTGTGATCTCAGCAGAGAAGGCCTACCACGAGCAGCTCTCTGTGGCAGAAATCACCAACAGTGCCTTTGAGCCGGCGTCTATGATGGCAAAATGTGACCCGAGGCACGGGAAGTACATGGCCTGCTGTTTAATGTACCGGGGAGATGTGGTTCCGAAAGACGTCAACGCAGCTGTGGCCACAATCAAGACCAAGAGGACAATTCAGTTTGTGGACTGGTGCCCGACTGGGTTCAAGTGCGGCATCAACTACCAGCCTCCTACTGTTGTTCCTGGTGGCGACTTGGCCAAAGTTCAGAGGGCTGTGTGCATGATTTCCAACTCTACTAGTGTTGCCGAAGTGTTTTCGAGGATTGATCACAAGTTTGATCTCATGTACGCCAAGCGCGCTTTCGTGCATTGGTATGTTGGCGAGGGCATGGAGGAAGGAGAGTTTTCCGAGGCTCGGGAGGACTTGGCGGCGCTAGAGAAGGACTATGAGGAAGTTGGGCTTGAGTCTGCTGAAGGTGATGATGGTGAAGATGATGAGTATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04145 ko04210 ko04530 ko04540 ko05130 map04145 map04210 map04530 map04540 map05130
Pfam Domains
Protein Families
Tubulin Tubulin_C

Protein Analysis

451

Amino Acids

49.64

Weight (kDa)

4.96

Isoelectric Point (pI)

36.69

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000549)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G04820 AT1G50010 AT4G14960 AT4G14960
fragaria_vesca FvH4_1g18660 FvH4_2g03140 FvH4_5g33560 FvH4_6g48940 FvH4_6g48940
malus_domestica MD02G1204800.v1.1 MD05G1106800.v1.1 MD08G1210600.v1.1 MD09G1049500.v1.1 MD10G1111600.v1.1 MD15G1398700.v1.1 MD17G1049700.v1.1
prunus_persica Prupe.1G542600_v2.0.a1 Prupe.3G269300_v2.0.a1 Prupe.6G212700_v2.0.a1 Prupe.8G150900_v2.0.a1
pyrus_communis pycom05g10370 pycom08g18150 pycom10g09640 pycom10g09660 pycom111g04050 pycom15g35470 pycom17g04460
rosa_chinensis RchiOBHm_Chr2g0109481 RchiOBHm_Chr2g0168751 RchiOBHm_Chr3g0453071 RchiOBHm_Chr3g0460741 RchiOBHm_Chr3g0467971 RchiOBHm_Chr4g0400901 RchiOBHm_Chr6g0248011 RchiOBHm_Chr7g0232381 RchiOBHm_Chr7g0238121
rosa_laevigata RLG00000001350 RLG00000009167 RLG00000015163 RLG00000017708 RLG00000017741 RLG00000021807 RLG00000023849 RLG00000026518
rosa_multiflora Rmu_sc0000332.1_g000021 Rmu_sc0000332.1_g000029 Rmu_sc0002416.1_g000007 Rmu_sc0002611.1_g000001 Rmu_sc0003113.1_g000016 Rmu_sc0004780.1_g000001 Rmu_sc0004805.1_g000035 Rmu_sc0010633.1_g000010 Rmu_sc0011069.1_g000003 Rmu_sc0016170.1_g000002 Rmu_sc0025757.1_g000003 Rmu_ssc0000484.1_g000003
rosa_roxburghii Rroxscaffold_2G00134200 Rroxscaffold_4G00321030 Rroxscaffold_7G00213500
rosa_rugosa Rorug01G0283100 Rorug02G0160500 Rorug02G0535300 Rorug04G0027000 Rorug04G0328100 Rorug05G0388700 Rorug05G0530200 Rorug06G0489200 Rorug07G0104900 Rorug07G0209400 Rorug07G0274500
rosa_samantha Rh2BG222800 Rh2BG618900 Rh2CG214400 Rh2CG587200 Rh6BG040200 Rh6CG206500 Rh6DG035600 Rh7BG401600 Rh7BG401700 Rh7DG418400
rosa_wichuraiana Rw2G050240 Rw6G003920 Rw7G035430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 122
AatII GACGTC 1 cut(s) 984
Acc36I ACCTGC 1 cut(s) 633
AccB1I GGYRCC 1 cut(s) 1038
AccI GTMKAC 1 cut(s) 513
AccII CGCG 1 cut(s) 1206
AciI CCGC 8 cut(s) 132, 250, 282, 297, 640, 717, 1059, 1277
AclWI GGATC 3 cut(s) 213, 354, 366
AcoI YGGCCR 4 cut(s) 28, 100, 996, 1104
AcuI CTGAAG 2 cut(s) 142, 1341
AcyI GRCGYC 4 cut(s) 141, 294, 896, 981
AfaI GTAC 5 cut(s) 554, 564, 935, 956, 1196
AfiI CCNNNNNNNGG 2 cut(s) 926, 1263
AgsI TTSAA 4 cut(s) 148, 398, 659, 1054
AhlI ACTAGT 1 cut(s) 1145
AjiI CACGTC 2 cut(s) 184, 754
AjnI CCWGG 5 cut(s) 30, 354, 405, 459, 1090
AjuI GAANNNNNNNTTGG 2 cut(s) 1193, 1225
AleI CACNNNNGTG 1 cut(s) 596
AloI GAACNNNNNNTCC 4 cut(s) 72, 104, 954, 986
AluBI AGCT 6 cut(s) 67, 256, 274, 373, 856, 992
AluI AGCT 6 cut(s) 67, 256, 274, 373, 856, 992
Alw21I GWGCWC 2 cut(s) 69, 591
Alw26I GTCTC 5 cut(s) 129, 525, 535, 651, 697
AlwI GGATC 3 cut(s) 213, 354, 366
Ama87I CYCGRG 3 cut(s) 500, 919, 1263
AoxI GGCC 9 cut(s) 28, 33, 100, 316, 442, 840, 939, 996, 1104
ApeKI GCWGC 2 cut(s) 853, 989
AspLEI GCGC 4 cut(s) 742, 1206, 1208, 1282
AspS9I GGNCC 2 cut(s) 237, 778
AsuC2I CCSGG 2 cut(s) 266, 959
AsuHPI GGTGA 4 cut(s) 706, 865, 1337, 1346
AvaI CYCGRG 3 cut(s) 500, 919, 1263
AvaII GGWCC 2 cut(s) 237, 778
AxyI CCTNAGG 1 cut(s) 525
BaeGI GKGCMC 1 cut(s) 1043
BalI TGGCCA 3 cut(s) 102, 998, 1106
BanI GGYRCC 1 cut(s) 1038
BanII GRGCYC 2 cut(s) 69, 216
BauI CACGAG 1 cut(s) 848
BbsI GAAGAC 2 cut(s) 297, 386
Bbv12I GWGCWC 2 cut(s) 69, 591
BbvI GCAGC 2 cut(s) 865, 1001
BccI CCATC 6 cut(s) 26, 92, 632, 728, 898, 1325
BceAI ACGGC 1 cut(s) 535
BciT130I CCWGG 5 cut(s) 32, 356, 407, 461, 1092
BclI TGATCA 1 cut(s) 1174
BcnI CCSGG 2 cut(s) 266, 959
BcoDI GTCTC 5 cut(s) 129, 525, 535, 651, 697
BcuI ACTAGT 1 cut(s) 1145
BfaI CTAG 3 cut(s) 650, 1146, 1283
BfoI RGCGCY 2 cut(s) 743, 1283
BfuAI ACCTGC 1 cut(s) 633
BisI GCNGC 7 cut(s) 283, 297, 640, 854, 990, 1060, 1278
BlsI GCNGC 7 cut(s) 284, 298, 641, 855, 991, 1061, 1279
Bme1390I CCNGG 7 cut(s) 32, 266, 356, 407, 461, 959, 1092
Bme18I GGWCC 2 cut(s) 237, 778
BmeT110I CYCGRG 3 cut(s) 500, 919, 1263
BmgBI CACGTC 2 cut(s) 184, 754
BmgT120I GGNCC 2 cut(s) 237, 778
BmiI GGNNCC 5 cut(s) 172, 404, 780, 972, 1040
BmrFI CCNGG 7 cut(s) 32, 266, 356, 407, 461, 959, 1092
BmrI ACTGGG 1 cut(s) 1056
BmsI GCATC 3 cut(s) 21, 96, 1071
BmuI ACTGGG 1 cut(s) 1056
BpiI GAAGAC 2 cut(s) 297, 386
BplI GAGNNNNNCTC 3 cut(s) 31, 842, 874
BpmI CTGGAG 1 cut(s) 189
BpuEI CTTGAG 2 cut(s) 230, 1331
BpuMI CCSGG 2 cut(s) 266, 959
Bsa29I ATCGAT 1 cut(s) 225
BsaHI GRCGYC 4 cut(s) 141, 294, 896, 981
BsaI GGTCTC 3 cut(s) 535, 651, 697
BsaJI CCNNGG 4 cut(s) 445, 920, 958, 1257
BsaXI ACNNNNNCTCC 2 cut(s) 954, 984
Bsc4I CCNNNNNNNGG 2 cut(s) 926, 1263
Bse118I RCCGGY 2 cut(s) 239, 892
Bse1I ACTGG 5 cut(s) 172, 394, 439, 1040, 1051
Bse21I CCTNAGG 1 cut(s) 525
BseBI CCWGG 5 cut(s) 32, 356, 407, 461, 1092
BseCI ATCGAT 1 cut(s) 225
BseDI CCNNGG 4 cut(s) 445, 920, 958, 1257
BseGI GGATG 2 cut(s) 18, 87
BseLI CCNNNNNNNGG 2 cut(s) 926, 1263
BseMII CTCAG 3 cut(s) 539, 710, 843
BseNI ACTGG 5 cut(s) 172, 394, 439, 1040, 1051
BsePI GCGCGC 1 cut(s) 1204
BseRI GAGGAG 1 cut(s) 513
BseSI GKGCMC 1 cut(s) 1043
BseXI GCAGC 2 cut(s) 865, 1001
BseYI CCCAGC 1 cut(s) 60
BsgI GTGCAG 1 cut(s) 370
Bsh1236I CGCG 1 cut(s) 1206
BshFI GGCC 9 cut(s) 30, 35, 102, 318, 444, 842, 941, 998, 1106
BshNI GGYRCC 1 cut(s) 1038
BshVI ATCGAT 1 cut(s) 225
BsiHKAI GWGCWC 2 cut(s) 69, 591
BsiHKCI CYCGRG 3 cut(s) 500, 919, 1263
BsiSI CCGG 6 cut(s) 36, 174, 240, 266, 893, 958
BslFI GGGAC 3 cut(s) 170, 445, 764
BslI CCNNNNNNNGG 2 cut(s) 926, 1263
BsmAI GTCTC 5 cut(s) 129, 525, 535, 651, 697
BsmBI CGTCTC 2 cut(s) 129, 525
BsmFI GGGAC 3 cut(s) 170, 445, 764
BsnI GGCC 9 cut(s) 30, 35, 102, 318, 444, 842, 941, 998, 1106
Bso31I GGTCTC 3 cut(s) 535, 651, 697
BsoBI CYCGRG 3 cut(s) 500, 919, 1263
Bsp1286I GDGCHC 4 cut(s) 69, 216, 591, 1043
Bsp143I GATC 6 cut(s) 205, 346, 358, 825, 1174, 1186
BspACI CCGC 8 cut(s) 132, 250, 282, 297, 640, 717, 1059, 1277
BspANI GGCC 9 cut(s) 30, 35, 102, 318, 444, 842, 941, 998, 1106
BspCNI CTCAG 3 cut(s) 538, 709, 842
BspDI ATCGAT 1 cut(s) 225
BspFNI CGCG 1 cut(s) 1206
BspLI GGNNCC 5 cut(s) 172, 404, 780, 972, 1040
BspMI ACCTGC 1 cut(s) 633
BspPI GGATC 3 cut(s) 213, 354, 366
BspT107I GGYRCC 1 cut(s) 1038
BspTNI GGTCTC 3 cut(s) 535, 651, 697
BsrFI RCCGGY 2 cut(s) 239, 892
BsrI ACTGG 5 cut(s) 172, 394, 439, 1040, 1051
BssAI RCCGGY 2 cut(s) 239, 892
BssECI CCNNGG 4 cut(s) 445, 920, 958, 1257
BssHII GCGCGC 1 cut(s) 1204
BssMI GATC 6 cut(s) 205, 346, 358, 825, 1174, 1186
BssNI GRCGYC 4 cut(s) 141, 294, 896, 981
BssSI CACGAG 1 cut(s) 848
Bst2BI CACGAG 1 cut(s) 848
Bst2UI CCWGG 5 cut(s) 32, 356, 407, 461, 1092
Bst4CI ACNGT 6 cut(s) 124, 220, 511, 567, 881, 1084
Bst6I CTCTTC 1 cut(s) 459
BstACI GRCGYC 4 cut(s) 141, 294, 896, 981
BstC8I GCNNGC 6 cut(s) 58, 254, 375, 894, 943, 1206
BstDEI CTNAG 3 cut(s) 525, 696, 829
BstF5I GGATG 2 cut(s) 18, 87
BstFNI CGCG 1 cut(s) 1206
BstH2I RGCGCY 2 cut(s) 743, 1283
BstHHI GCGC 4 cut(s) 742, 1206, 1208, 1282
BstKTI GATC 6 cut(s) 208, 349, 361, 828, 1177, 1189
BstMAI GTCTC 5 cut(s) 129, 525, 535, 651, 697
BstMBI GATC 6 cut(s) 205, 346, 358, 825, 1174, 1186
BstMWI GCNNNNNNNGC 4 cut(s) 249, 839, 995, 1205
BstNI CCWGG 5 cut(s) 32, 356, 407, 461, 1092
BstSCI CCNGG 7 cut(s) 30, 264, 354, 405, 459, 957, 1090
BstSLI GKGCMC 1 cut(s) 1043
BstUI CGCG 1 cut(s) 1206
BstV1I GCAGC 2 cut(s) 865, 1001
BstV2I GAAGAC 2 cut(s) 297, 386
BstX2I RGATCY 2 cut(s) 205, 346
BstXI CCANNNNNNTGG 2 cut(s) 98, 422
BstYI RGATCY 2 cut(s) 205, 346
Bsu15I ATCGAT 1 cut(s) 225
Bsu36I CCTNAGG 1 cut(s) 525
BsuRI GGCC 9 cut(s) 30, 35, 102, 318, 444, 842, 941, 998, 1106
BsuTUI ATCGAT 1 cut(s) 225
BtrI CACGTC 2 cut(s) 184, 754
BtsCI GGATG 2 cut(s) 18, 87
BtsIMutI CAGTG 3 cut(s) 387, 591, 886
BveI ACCTGC 1 cut(s) 633
Cac8I GCNNGC 6 cut(s) 58, 254, 375, 894, 943, 1206
CfoI GCGC 4 cut(s) 742, 1206, 1208, 1282
Cfr10I RCCGGY 2 cut(s) 239, 892
Cfr13I GGNCC 2 cut(s) 237, 778
ClaI ATCGAT 1 cut(s) 225
CseI GACGC 3 cut(s) 149, 302, 885
Csp6I GTAC 5 cut(s) 553, 563, 934, 955, 1195
CviAII CATG 6 cut(s) 83, 802, 937, 1129, 1192, 1237
CviQI GTAC 5 cut(s) 553, 563, 934, 955, 1195
DdeI CTNAG 3 cut(s) 525, 696, 829
DpnI GATC 6 cut(s) 207, 348, 360, 827, 1176, 1188
DpnII GATC 6 cut(s) 205, 346, 358, 825, 1174, 1186
DrdI GACNNNNNNGTC 1 cut(s) 122
DseDI GACNNNNNNGTC 1 cut(s) 122
EaeI YGGCCR 4 cut(s) 28, 100, 996, 1104
Eam1104I CTCTTC 1 cut(s) 459
EarI CTCTTC 1 cut(s) 459
EciI GGCGGA 1 cut(s) 147
Ecl136II GAGCTC 1 cut(s) 67
Eco147I AGGCCT 1 cut(s) 842
Eco24I GRGCYC 2 cut(s) 69, 216
Eco31I GGTCTC 3 cut(s) 535, 651, 697
Eco47I GGWCC 2 cut(s) 237, 778
Eco53kI GAGCTC 1 cut(s) 67
Eco57I CTGAAG 2 cut(s) 142, 1341
Eco81I CCTNAGG 1 cut(s) 525
Eco88I CYCGRG 3 cut(s) 500, 919, 1263
EcoICRI GAGCTC 1 cut(s) 67
EcoRII CCWGG 5 cut(s) 30, 354, 405, 459, 1090
EcoT38I GRGCYC 2 cut(s) 69, 216
Esp3I CGTCTC 2 cut(s) 129, 525
FaeI CATG 6 cut(s) 86, 805, 940, 1132, 1195, 1240
FalI AAGNNNNNCTT 4 cut(s) 652, 684, 1293, 1325
FaqI GGGAC 3 cut(s) 170, 445, 764
FatI CATG 6 cut(s) 82, 801, 936, 1128, 1191, 1236
FbaI TGATCA 1 cut(s) 1174
FblI GTMKAC 1 cut(s) 513
Fnu4HI GCNGC 7 cut(s) 283, 297, 640, 854, 990, 1060, 1278
FokI GGATG 2 cut(s) 5, 74
FriOI GRGCYC 2 cut(s) 69, 216
Fsp4HI GCNGC 7 cut(s) 283, 297, 640, 854, 990, 1060, 1278
FspBI CTAG 3 cut(s) 650, 1146, 1283
GlaI GCGC 4 cut(s) 741, 1205, 1207, 1281
GluI GCNGC 7 cut(s) 283, 297, 640, 854, 990, 1060, 1278
GsaI CCCAGC 1 cut(s) 64
GsuI CTGGAG 1 cut(s) 189
HaeII RGCGCY 2 cut(s) 743, 1283
HaeIII GGCC 9 cut(s) 30, 35, 102, 318, 444, 842, 941, 998, 1106
HapII CCGG 6 cut(s) 36, 174, 240, 266, 893, 958
HgaI GACGC 3 cut(s) 149, 302, 885
HhaI GCGC 4 cut(s) 742, 1206, 1208, 1282
Hin1I GRCGYC 4 cut(s) 141, 294, 896, 981
Hin1II CATG 6 cut(s) 86, 805, 940, 1132, 1195, 1240
Hin6I GCGC 4 cut(s) 740, 1204, 1206, 1280
HinP1I GCGC 4 cut(s) 740, 1204, 1206, 1280
HincII GTYRAC 1 cut(s) 985
HindII GTYRAC 1 cut(s) 985
HindIII AAGCTT 1 cut(s) 371
HinfI GANTC 4 cut(s) 39, 363, 792, 1313
HpaII CCGG 6 cut(s) 36, 174, 240, 266, 893, 958
HphI GGTGA 4 cut(s) 706, 865, 1337, 1346
Hpy166II GTNNAC 6 cut(s) 507, 514, 570, 751, 985, 1033
Hpy188I TCNGA 5 cut(s) 50, 368, 975, 1116, 1258
Hpy188III TCNNGA 6 cut(s) 209, 235, 650, 766, 1006, 1265
Hpy8I GTNNAC 6 cut(s) 507, 514, 570, 751, 985, 1033
Hpy99I CGWCG 2 cut(s) 128, 143
HpyAV CCTTC 6 cut(s) 154, 328, 832, 1238, 1282, 1316
HpyCH4III ACNGT 6 cut(s) 124, 220, 511, 567, 881, 1084
HpyCH4IV ACGT 4 cut(s) 183, 747, 753, 981
HpyCH4V TGCA 5 cut(s) 12, 387, 818, 1128, 1216
HpyF10VI GCNNNNNNNGC 4 cut(s) 249, 839, 995, 1205
HpyF3I CTNAG 3 cut(s) 525, 696, 829
HpySE526I ACGT 4 cut(s) 183, 747, 753, 981
Hsp92I GRCGYC 4 cut(s) 141, 294, 896, 981
Hsp92II CATG 6 cut(s) 86, 805, 940, 1132, 1195, 1240
HspAI GCGC 4 cut(s) 740, 1204, 1206, 1280
KroI GCCGGC 1 cut(s) 892
KroNI GCCGGC 1 cut(s) 894
Ksp22I TGATCA 1 cut(s) 1174
Kzo9I GATC 6 cut(s) 205, 346, 358, 825, 1174, 1186
LmnI GCTCC 3 cut(s) 64, 170, 586
Lsp1109I GCAGC 2 cut(s) 865, 1001
LweI GCATC 3 cut(s) 21, 96, 1071
MaeI CTAG 3 cut(s) 650, 1146, 1283
MaeII ACGT 4 cut(s) 183, 747, 753, 981
MaeIII GTNAC 3 cut(s) 113, 754, 914
MalI GATC 6 cut(s) 207, 348, 360, 827, 1176, 1188
MboI GATC 6 cut(s) 205, 346, 358, 825, 1174, 1186
MboII GAAGA 5 cut(s) 148, 302, 386, 446, 1349
MfeI CAATTG 1 cut(s) 327
MflI RGATCY 2 cut(s) 205, 346
MhlI GDGCHC 4 cut(s) 69, 216, 591, 1043
MlsI TGGCCA 3 cut(s) 102, 998, 1106
MluCI AATT 3 cut(s) 327, 772, 1020
MluNI TGGCCA 3 cut(s) 102, 998, 1106
MlyI GAGTC 1 cut(s) 1322
MmeI TCCRAC 3 cut(s) 28, 403, 1161
Mox20I TGGCCA 3 cut(s) 102, 998, 1106
MroNI GCCGGC 1 cut(s) 892
MscI TGGCCA 3 cut(s) 102, 998, 1106
MseI TTAA 1 cut(s) 950
MslI CAYNNNNRTG 3 cut(s) 594, 596, 1221
Msp20I TGGCCA 3 cut(s) 102, 998, 1106
MspA1I CMGCKG 2 cut(s) 282, 992
MspI CCGG 6 cut(s) 36, 174, 240, 266, 893, 958
MspR9I CCNGG 7 cut(s) 32, 266, 356, 407, 461, 959, 1092
MunI CAATTG 1 cut(s) 327
MvaI CCWGG 5 cut(s) 32, 356, 407, 461, 1092
MvnI CGCG 1 cut(s) 1206
MwoI GCNNNNNNNGC 4 cut(s) 249, 839, 995, 1205
NaeI GCCGGC 1 cut(s) 894
NciI CCSGG 2 cut(s) 266, 959
NdeII GATC 6 cut(s) 205, 346, 358, 825, 1174, 1186
NgoMIV GCCGGC 1 cut(s) 892
NlaIII CATG 6 cut(s) 86, 805, 940, 1132, 1195, 1240
NlaIV GGNNCC 5 cut(s) 172, 404, 780, 972, 1040
NmeAIII GCCGAG 2 cut(s) 135, 426
NmuCI GTSAC 3 cut(s) 113, 754, 914
OliI CACNNNNGTG 1 cut(s) 596
PauI GCGCGC 1 cut(s) 1204
PceI AGGCCT 1 cut(s) 842
PdiI GCCGGC 1 cut(s) 894
PfeI GAWTC 3 cut(s) 39, 363, 792
PfoI TCCNGGA 1 cut(s) 459
PkrI GCNGC 7 cut(s) 284, 298, 641, 855, 991, 1061, 1279
PleI GAGTC 1 cut(s) 1321
PpsI GAGTC 1 cut(s) 1321
Psp124BI GAGCTC 1 cut(s) 69
Psp6I CCWGG 5 cut(s) 30, 354, 405, 459, 1090
PspFI CCCAGC 1 cut(s) 60
PspGI CCWGG 5 cut(s) 30, 354, 405, 459, 1090
PspN4I GGNNCC 5 cut(s) 172, 404, 780, 972, 1040
PspPI GGNCC 2 cut(s) 237, 778
PsuI RGATCY 2 cut(s) 205, 346
PteI GCGCGC 1 cut(s) 1204
PvuII CAGCTG 1 cut(s) 992
RsaI GTAC 5 cut(s) 554, 564, 935, 956, 1196
RsaNI GTAC 5 cut(s) 553, 563, 934, 955, 1195
RseI CAYNNNNRTG 3 cut(s) 594, 596, 1221
SacI GAGCTC 1 cut(s) 69
SaqAI TTAA 1 cut(s) 950
SatI GCNGC 7 cut(s) 283, 297, 640, 854, 990, 1060, 1278
Sau3AI GATC 6 cut(s) 205, 346, 358, 825, 1174, 1186
Sau96I GGNCC 2 cut(s) 237, 778
SchI GAGTC 1 cut(s) 1322
ScrFI CCNGG 7 cut(s) 32, 266, 356, 407, 461, 959, 1092
SduI GDGCHC 4 cut(s) 69, 216, 591, 1043
SfaNI GCATC 3 cut(s) 21, 96, 1071
SinI GGWCC 2 cut(s) 237, 778
SmiMI CAYNNNNRTG 3 cut(s) 594, 596, 1221
SmlI CTYRAG 2 cut(s) 209, 1310
SmoI CTYRAG 2 cut(s) 209, 1310
SpeI ACTAGT 1 cut(s) 1145
Sse9I AATT 3 cut(s) 327, 772, 1020
SseBI AGGCCT 1 cut(s) 842
SsiI CCGC 8 cut(s) 132, 250, 282, 297, 640, 717, 1059, 1277
SspMI CTAG 3 cut(s) 650, 1146, 1283
SstI GAGCTC 1 cut(s) 69
StuI AGGCCT 1 cut(s) 842
StyD4I CCNGG 7 cut(s) 30, 264, 354, 405, 459, 957, 1090
TaaI ACNGT 6 cut(s) 124, 220, 511, 567, 881, 1084
TaiI ACGT 4 cut(s) 186, 750, 756, 984
TaqI TCGA 5 cut(s) 78, 225, 361, 494, 1166
TasI AATT 3 cut(s) 327, 772, 1020
TatI WGTACW 1 cut(s) 933
TauI GCSGC 5 cut(s) 285, 299, 642, 1062, 1280
TfiI GAWTC 3 cut(s) 39, 363, 792
Tru1I TTAA 1 cut(s) 950
Tru9I TTAA 1 cut(s) 950
TscAI CASTG 3 cut(s) 394, 598, 886
TseFI GTSAC 3 cut(s) 113, 754, 914
TseI GCWGC 2 cut(s) 853, 989
Tsp45I GTSAC 3 cut(s) 113, 754, 914
TspDTI ATGAA 1 cut(s) 790
TspGWI ACGGA 1 cut(s) 498
TspRI CASTG 3 cut(s) 394, 598, 886
VpaK11BI GGWCC 2 cut(s) 237, 778
XbaI TCTAGA 1 cut(s) 649
XcmI CCANNNNNNNNNTGG 1 cut(s) 326
XmiI GTMKAC 1 cut(s) 513
XspI CTAG 3 cut(s) 650, 1146, 1283
ZraI GACGTC 1 cut(s) 982
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.