Rorug01G0283100

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0000549
Physical Location & Seq
Reverse (-)
39636869 .. 39637550
682 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0283100.1

Sequence Viewer

Length: 612 bp
ATGCCTGTTCTGGATGCTGATCGGCTCTTGCCAGAAGGCGCCGAGGCCACCGTAGTGCCTTTTGAAAAGATCCACCACTTGAAGGTCGCGTTTGATCTAGTAGAACTGCCCTTCAAGCACTTCGTTTCTGATAAAGTGCCTGTTGGATACCAGCTAGTTGATTTTGCAGCTCACTGGGTGGTTGACACGGCTAAAGAGTATGAACATCTAAATCAAGGTCCGCCATCACCCGGAAAGCTGGACATCCCTGACGGACTGGGTCCCCTTTCCGTCGACCTTGTAGCGTTTGAAGAGTATGAGACGGTTCCTGTGCATCAGGGTTTTATGGTGCAAATGGTTCTGGGATATATAAGTGATGCTGTAAGGCTTGCCAAGATTGTATCATCGAGTCAAGTACTAGCCGTTGGAACTTGCAACGAATTTGAAGAAGAATACTTGGATGTTTACGCAAAAATCACTAGAAAACATGTGATTCCCACAGGTTTGCTATCTCCAGAGAAATCAAATAGGGATAACATCACTGTGGGTTCAGGGCGTTATGTGATGTTTGATTGGCTTGATAAGCAAAATCCTAGGTCAGTTGATTTTGCTATCTCCAGTGAACCCACATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04145 ko04210 ko04530 ko04540 ko05130 map04145 map04210 map04530 map04540 map05130
Pfam Domains
Protein Families
Tubulin Tubulin_C

Protein Analysis

203

Amino Acids

22.58

Weight (kDa)

4.84

Isoelectric Point (pI)

29.97

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000549)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G04820 AT1G50010 AT4G14960 AT4G14960
fragaria_vesca FvH4_1g18660 FvH4_2g03140 FvH4_5g33560 FvH4_6g48940 FvH4_6g48940
malus_domestica MD02G1204800.v1.1 MD05G1106800.v1.1 MD08G1210600.v1.1 MD09G1049500.v1.1 MD10G1111600.v1.1 MD15G1398700.v1.1 MD17G1049700.v1.1
prunus_persica Prupe.1G542600_v2.0.a1 Prupe.3G269300_v2.0.a1 Prupe.6G212700_v2.0.a1 Prupe.8G150900_v2.0.a1
pyrus_communis pycom05g10370 pycom08g18150 pycom10g09640 pycom10g09660 pycom111g04050 pycom15g35470 pycom17g04460
rosa_chinensis RchiOBHm_Chr2g0109481 RchiOBHm_Chr2g0168751 RchiOBHm_Chr3g0453071 RchiOBHm_Chr3g0460741 RchiOBHm_Chr3g0467971 RchiOBHm_Chr4g0400901 RchiOBHm_Chr6g0248011 RchiOBHm_Chr7g0232381 RchiOBHm_Chr7g0238121
rosa_laevigata RLG00000001350 RLG00000009167 RLG00000015163 RLG00000017708 RLG00000017741 RLG00000021807 RLG00000023849 RLG00000026518
rosa_multiflora Rmu_sc0000332.1_g000021 Rmu_sc0000332.1_g000029 Rmu_sc0002416.1_g000007 Rmu_sc0002611.1_g000001 Rmu_sc0003113.1_g000016 Rmu_sc0004780.1_g000001 Rmu_sc0004805.1_g000035 Rmu_sc0010633.1_g000010 Rmu_sc0011069.1_g000003 Rmu_sc0016170.1_g000002 Rmu_sc0025757.1_g000003 Rmu_ssc0000484.1_g000003
rosa_roxburghii Rroxscaffold_2G00134200 Rroxscaffold_4G00321030 Rroxscaffold_7G00213500
rosa_rugosa Rorug01G0283100 Rorug02G0160500 Rorug02G0535300 Rorug04G0027000 Rorug04G0328100 Rorug05G0388700 Rorug05G0530200 Rorug06G0489200 Rorug07G0104900 Rorug07G0209400 Rorug07G0274500
rosa_samantha Rh2BG222800 Rh2BG618900 Rh2CG214400 Rh2CG587200 Rh6BG040200 Rh6CG206500 Rh6DG035600 Rh7BG401600 Rh7BG401700 Rh7DG418400
rosa_wichuraiana Rw2G050240 Rw6G003920 Rw7G035430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 38
AccI GTMKAC 1 cut(s) 273
AccII CGCG 1 cut(s) 89
AciI CCGC 1 cut(s) 221
AclWI GGATC 1 cut(s) 64
AcsI RAATTY 1 cut(s) 419
AcyI GRCGYC 1 cut(s) 39
AdeI CACNNNGTG 1 cut(s) 178
AfaI GTAC 1 cut(s) 396
AfiI CCNNNNNNNGG 2 cut(s) 82, 230
AflIII ACRYGT 1 cut(s) 466
AgsI TTSAA 5 cut(s) 65, 82, 115, 290, 425
AleI CACNNNNGTG 1 cut(s) 53
AluBI AGCT 3 cut(s) 154, 170, 238
AluI AGCT 3 cut(s) 154, 170, 238
Alw26I GTCTC 1 cut(s) 293
AlwI GGATC 1 cut(s) 64
AoxI GGCC 1 cut(s) 45
ApeKI GCWGC 1 cut(s) 167
ApoI RAATTY 1 cut(s) 419
AspA2I CCTAGG 1 cut(s) 572
AspLEI GCGC 1 cut(s) 41
AspS9I GGNCC 2 cut(s) 218, 260
AsuC2I CCSGG 1 cut(s) 231
AsuHPI GGTGA 1 cut(s) 219
AvaII GGWCC 2 cut(s) 218, 260
AvrII CCTAGG 1 cut(s) 572
BanI GGYRCC 1 cut(s) 38
BbvI GCAGC 1 cut(s) 179
BccI CCATC 1 cut(s) 232
BceAI ACGGC 2 cut(s) 204, 386
BciVI GTATCC 1 cut(s) 140
BcnI CCSGG 1 cut(s) 231
BcoDI GTCTC 1 cut(s) 293
BfaI CTAG 5 cut(s) 98, 155, 398, 459, 573
BfoI RGCGCY 1 cut(s) 42
BfuI GTATCC 1 cut(s) 140
BisI GCNGC 1 cut(s) 168
BlnI CCTAGG 1 cut(s) 572
BlsI GCNGC 1 cut(s) 169
BmcAI AGTACT 1 cut(s) 396
Bme1390I CCNGG 1 cut(s) 231
Bme18I GGWCC 2 cut(s) 218, 260
BmgT120I GGNCC 2 cut(s) 218, 260
BmiI GGNNCC 4 cut(s) 40, 261, 262, 306
BmrFI CCNGG 1 cut(s) 231
BmrI ACTGGG 2 cut(s) 184, 266
BmsI GCATC 3 cut(s) 4, 322, 346
BmuI ACTGGG 2 cut(s) 184, 266
BpmI CTGGAG 2 cut(s) 477, 580
BpuMI CCSGG 1 cut(s) 231
BsaBI GATNNNNATC 1 cut(s) 18
BsaHI GRCGYC 1 cut(s) 39
BsaJI CCNNGG 2 cut(s) 42, 572
Bsc4I CCNNNNNNNGG 2 cut(s) 82, 230
Bse1I ACTGG 3 cut(s) 179, 261, 597
Bse8I GATNNNNATC 1 cut(s) 18
BseDI CCNNGG 2 cut(s) 42, 572
BseGI GGATG 3 cut(s) 19, 243, 445
BseJI GATNNNNATC 1 cut(s) 18
BseLI CCNNNNNNNGG 2 cut(s) 82, 230
BseNI ACTGG 3 cut(s) 179, 261, 597
BseXI GCAGC 1 cut(s) 179
Bsh1236I CGCG 1 cut(s) 89
BshFI GGCC 1 cut(s) 47
BshNI GGYRCC 1 cut(s) 38
BsiSI CCGG 1 cut(s) 231
BslFI GGGAC 1 cut(s) 246
BslI CCNNNNNNNGG 2 cut(s) 82, 230
BsmAI GTCTC 1 cut(s) 293
BsmBI CGTCTC 1 cut(s) 293
BsmFI GGGAC 1 cut(s) 246
BsnI GGCC 1 cut(s) 47
Bsp143I GATC 3 cut(s) 19, 69, 94
BspACI CCGC 1 cut(s) 221
BspANI GGCC 1 cut(s) 47
BspFNI CGCG 1 cut(s) 89
BspLI GGNNCC 4 cut(s) 40, 261, 262, 306
BspPI GGATC 1 cut(s) 64
BspT107I GGYRCC 1 cut(s) 38
BsrI ACTGG 3 cut(s) 179, 261, 597
BssECI CCNNGG 2 cut(s) 42, 572
BssMI GATC 3 cut(s) 19, 69, 94
BssNI GRCGYC 1 cut(s) 39
BssT1I CCWWGG 1 cut(s) 572
Bst4CI ACNGT 3 cut(s) 52, 304, 523
Bst6I CTCTTC 1 cut(s) 285
BstACI GRCGYC 1 cut(s) 39
BstC8I GCNNGC 1 cut(s) 369
BstF5I GGATG 3 cut(s) 19, 243, 445
BstFNI CGCG 1 cut(s) 89
BstH2I RGCGCY 1 cut(s) 42
BstHHI GCGC 1 cut(s) 41
BstKTI GATC 3 cut(s) 22, 72, 97
BstMAI GTCTC 1 cut(s) 293
BstMBI GATC 3 cut(s) 19, 69, 94
BstMWI GCNNNNNNNGC 2 cut(s) 115, 562
BstNSI RCATGY 1 cut(s) 470
BstSCI CCNGG 1 cut(s) 229
BstUI CGCG 1 cut(s) 89
BstV1I GCAGC 1 cut(s) 179
BstX2I RGATCY 1 cut(s) 69
BstYI RGATCY 1 cut(s) 69
BsuI GTATCC 1 cut(s) 140
BsuRI GGCC 1 cut(s) 47
BtsCI GGATG 3 cut(s) 19, 243, 445
BtsIMutI CAGTG 3 cut(s) 172, 519, 604
Cac8I GCNNGC 1 cut(s) 369
CfoI GCGC 1 cut(s) 41
Cfr13I GGNCC 2 cut(s) 218, 260
Csp6I GTAC 1 cut(s) 395
CviAII CATG 1 cut(s) 467
CviJI RGCY 9 cut(s) 25, 47, 154, 170, 191, 238, 367, 401, 556
CviKI_1 RGCY 9 cut(s) 25, 47, 154, 170, 191, 238, 367, 401, 556
CviQI GTAC 1 cut(s) 395
DinI GGCGCC 1 cut(s) 40
DpnI GATC 3 cut(s) 21, 71, 96
DpnII GATC 3 cut(s) 19, 69, 94
DraIII CACNNNGTG 1 cut(s) 178
Eam1104I CTCTTC 1 cut(s) 285
EarI CTCTTC 1 cut(s) 285
EciI GGCGGA 1 cut(s) 210
Eco130I CCWWGG 1 cut(s) 572
Eco47I GGWCC 2 cut(s) 218, 260
EcoO109I RGGNCCY 1 cut(s) 260
EcoT14I CCWWGG 1 cut(s) 572
EgeI GGCGCC 1 cut(s) 40
EheI GGCGCC 1 cut(s) 40
ErhI CCWWGG 1 cut(s) 572
Esp3I CGTCTC 1 cut(s) 293
FaeI CATG 1 cut(s) 470
FaiI YATR 8 cut(s) 201, 297, 326, 348, 350, 468, 540, 610
FaqI GGGAC 1 cut(s) 246
FatI CATG 1 cut(s) 466
FblI GTMKAC 1 cut(s) 273
Fnu4HI GCNGC 1 cut(s) 168
FokI GGATG 3 cut(s) 26, 230, 452
Fsp4HI GCNGC 1 cut(s) 168
FspBI CTAG 5 cut(s) 98, 155, 398, 459, 573
GlaI GCGC 1 cut(s) 40
GluI GCNGC 1 cut(s) 168
GsuI CTGGAG 2 cut(s) 477, 580
HaeII RGCGCY 1 cut(s) 42
HaeIII GGCC 1 cut(s) 47
HapII CCGG 1 cut(s) 231
HhaI GCGC 1 cut(s) 41
Hin1I GRCGYC 1 cut(s) 39
Hin1II CATG 1 cut(s) 470
Hin6I GCGC 1 cut(s) 39
HinP1I GCGC 1 cut(s) 39
HincII GTYRAC 2 cut(s) 184, 274
HindII GTYRAC 2 cut(s) 184, 274
HinfI GANTC 2 cut(s) 388, 472
HpaII CCGG 1 cut(s) 231
HphI GGTGA 1 cut(s) 219
Hpy166II GTNNAC 4 cut(s) 184, 274, 445, 602
Hpy188I TCNGA 1 cut(s) 130
Hpy188III TCNNGA 2 cut(s) 11, 494
Hpy8I GTNNAC 4 cut(s) 184, 274, 445, 602
Hpy99I CGWCG 1 cut(s) 275
HpyAV CCTTC 3 cut(s) 29, 76, 121
HpyCH4III ACNGT 3 cut(s) 52, 304, 523
HpyCH4V TGCA 4 cut(s) 167, 313, 331, 414
HpyF10VI GCNNNNNNNGC 2 cut(s) 115, 562
Hsp92I GRCGYC 1 cut(s) 39
Hsp92II CATG 1 cut(s) 470
HspAI GCGC 1 cut(s) 39
KasI GGCGCC 1 cut(s) 38
KflI GGGWCCC 1 cut(s) 260
Kzo9I GATC 3 cut(s) 19, 69, 94
Lsp1109I GCAGC 1 cut(s) 179
LweI GCATC 3 cut(s) 4, 322, 346
MaeI CTAG 5 cut(s) 98, 155, 398, 459, 573
MalI GATC 3 cut(s) 21, 71, 96
MboI GATC 3 cut(s) 19, 69, 94
MboII GAAGA 3 cut(s) 302, 437, 440
MflI RGATCY 1 cut(s) 69
MluCI AATT 1 cut(s) 419
Mly113I GGCGCC 1 cut(s) 39
MlyI GAGTC 1 cut(s) 397
MmeI TCCRAC 2 cut(s) 124, 385
MnlI CCTC 1 cut(s) 37
MslI CAYNNNNRTG 2 cut(s) 53, 521
MspI CCGG 1 cut(s) 231
MspR9I CCNGG 1 cut(s) 231
MvnI CGCG 1 cut(s) 89
MwoI GCNNNNNNNGC 2 cut(s) 115, 562
NarI GGCGCC 1 cut(s) 39
NciI CCSGG 1 cut(s) 231
NdeII GATC 3 cut(s) 19, 69, 94
NlaIII CATG 1 cut(s) 470
NlaIV GGNNCC 4 cut(s) 40, 261, 262, 306
NmeAIII GCCGAG 1 cut(s) 67
NspI RCATGY 1 cut(s) 470
OliI CACNNNNGTG 1 cut(s) 53
PciI ACATGT 1 cut(s) 466
PfeI GAWTC 1 cut(s) 472
PflFI GACNNNGTC 1 cut(s) 258
PkrI GCNGC 1 cut(s) 169
PleI GAGTC 1 cut(s) 396
PluTI GGCGCC 1 cut(s) 42
PpsI GAGTC 1 cut(s) 396
PpuMI RGGWCCY 1 cut(s) 260
PscI ACATGT 1 cut(s) 466
Psp5II RGGWCCY 1 cut(s) 260
PspN4I GGNNCC 4 cut(s) 40, 261, 262, 306
PspPI GGNCC 2 cut(s) 218, 260
PspPPI RGGWCCY 1 cut(s) 260
PsuI RGATCY 1 cut(s) 69
PsyI GACNNNGTC 1 cut(s) 258
RsaI GTAC 1 cut(s) 396
RsaNI GTAC 1 cut(s) 395
RseI CAYNNNNRTG 2 cut(s) 53, 521
SalI GTCGAC 1 cut(s) 272
SatI GCNGC 1 cut(s) 168
Sau3AI GATC 3 cut(s) 19, 69, 94
Sau96I GGNCC 2 cut(s) 218, 260
ScaI AGTACT 1 cut(s) 396
SchI GAGTC 1 cut(s) 397
ScrFI CCNGG 1 cut(s) 231
SetI ASST 8 cut(s) 87, 156, 172, 220, 240, 279, 484, 578
SfaNI GCATC 3 cut(s) 4, 322, 346
SfoI GGCGCC 1 cut(s) 40
SinI GGWCC 2 cut(s) 218, 260
SmiMI CAYNNNNRTG 2 cut(s) 53, 521
Sse9I AATT 1 cut(s) 419
SsiI CCGC 1 cut(s) 221
SspDI GGCGCC 1 cut(s) 38
SspMI CTAG 5 cut(s) 98, 155, 398, 459, 573
StyD4I CCNGG 1 cut(s) 229
StyI CCWWGG 1 cut(s) 572
TaaI ACNGT 3 cut(s) 52, 304, 523
TaqI TCGA 2 cut(s) 273, 386
TasI AATT 1 cut(s) 419
TatI WGTACW 1 cut(s) 394
TfiI GAWTC 1 cut(s) 472
TscAI CASTG 3 cut(s) 179, 526, 604
TseI GCWGC 1 cut(s) 167
TspDTI ATGAA 1 cut(s) 216
TspGWI ACGGA 2 cut(s) 259, 267
TspRI CASTG 3 cut(s) 179, 526, 604
Tth111I GACNNNGTC 1 cut(s) 258
VpaK11BI GGWCC 2 cut(s) 218, 260
XapI RAATTY 1 cut(s) 419
XceI RCATGY 1 cut(s) 470
XmaJI CCTAGG 1 cut(s) 572
XmiI GTMKAC 1 cut(s) 273
XspI CTAG 5 cut(s) 98, 155, 398, 459, 573
ZrmI AGTACT 1 cut(s) 396
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.