FvH4_6g48940

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0000549
Physical Location & Seq
Reverse (-)
36736698 .. 36739763
3066 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g48940.t1

Sequence Viewer

Length: 1353 bp
ATGAGAGAGTGCATCTCCATCCACATCGGCCAAGCCGGAATCCAAGTCGGCAACGCCTGCTGGGAGCTTTACTGCCTCGAACACGGCATTCAGCCTGATGGCCAAATGCCGAGTGACAAGACCGTCGGCGGCGGAGACGACGCCTTCAACACTTTCTTCAGCGAAACCGGCGCCGGAAAGCACGTCCCCCGCGCCGTGTTCTTGGATCTGGAGCCCACCGTCATCGACGAGGTGAGGACCGGAACTTACCGTCAGCTCTTCCACCCTGAGCAGCTCATCAGCGGCAAGGAGGACGCCGCCAACAACTTCGCCAGAGGCCACTACACAATCGGGAAGGAGATTGTGGATCTGTGCTTGGACAGGATCCGGAAGCTTGCGGACAACTGTACTGGGCTTCAAGGGTTTCTGGTGTTCCACGCTGTCGGAGGTGGGACTGGATCTGGACTTGGATCTCTGCTCTTGGAGAGGCTCTCTGTCGACTATGGGAAGAAATCTAAGCTTGGATTTACTGTGTACCCATCTCCTCAGGTCTCTACTTCTGTTGTGGAGCCTTACAACAGTGTCTTGTCCACTCACTCGCTCTTGGAGCACACTGATGTGGCTGTGCTTTTGGACAATGAAGCCATCTATGATATCTGCCGTAGGTCGCTTGACATTGAGAGACCTACCTACACCAACCTCAACAGGCTTGTTTCTCAGGTTATTTCCTCACTCACTGCTTCTCTGCGTTTTGATGGAGCACTCAATGTGGATGTGACTGAGTTCCAGACCAATCTGGTGCCATACCCCAGAATCCATTTCATGCTTTCATCCTATGCACCAGTCATCTCTGCCGAGAAGGCCTACCATGAGCAACTCTCTGTGGCTGAAATCACCAACAGCGCCTTTGAGCCAGCCTCTATGATGGCCAAATGTGATCCTAGGCATGGGAAGTACATGGCTTGCTGTTTGATGTACAGAGGAGATGTCGTACCAAAGGATGTGAATGCGGCTGTGGCCACAATCAAGACCAAGAGGACCATTCAGTTTGTGGATTGGTGCCCAACTGGTTTCAAGTGTGGTATCAACTACCAGCCTCCTACTGTTGTACCTGGTGGTGACTTGGCCAAGGTTCAGAGGGCTGTGTGCATGATTTCCAACTCCACAAGTGTTGCTGAGGTGTTTTCAAGGATTGATCACAAGTTTGATCTGATGTATGCCAAGCGTGCTTTTGTGCACTGGTATGTTGGTGAGGGTATGGAGGAGGGTGAGTTCTCAGAAGCTAGGGAGGACTTGGCTGCTCTTGAGAAGGATTATGAAGAAGTTGGGTTGGAGTCTGCTGAAGGTGAAGATGGTGAGGATGAAGAGTACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04145 ko04210 ko04530 ko04540 ko05130 map04145 map04210 map04530 map04540 map05130
Pfam Domains
Protein Families
Tubulin Tubulin_C

Protein Analysis

451

Amino Acids

49.65

Weight (kDa)

4.97

Isoelectric Point (pI)

37.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tubulin PF00091 3 - 213 6.2e-63 Tubulin/FtsZ family, GTPase domain
Tubulin_C PF03953 263 - 392 8e-52 Tubulin C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000549)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G04820 AT1G50010 AT4G14960 AT4G14960
fragaria_vesca FvH4_1g18660 FvH4_2g03140 FvH4_5g33560 FvH4_6g48940 FvH4_6g48940
malus_domestica MD02G1204800.v1.1 MD05G1106800.v1.1 MD08G1210600.v1.1 MD09G1049500.v1.1 MD10G1111600.v1.1 MD15G1398700.v1.1 MD17G1049700.v1.1
prunus_persica Prupe.1G542600_v2.0.a1 Prupe.3G269300_v2.0.a1 Prupe.6G212700_v2.0.a1 Prupe.8G150900_v2.0.a1
pyrus_communis pycom05g10370 pycom08g18150 pycom10g09640 pycom10g09660 pycom111g04050 pycom15g35470 pycom17g04460
rosa_chinensis RchiOBHm_Chr2g0109481 RchiOBHm_Chr2g0168751 RchiOBHm_Chr3g0453071 RchiOBHm_Chr3g0460741 RchiOBHm_Chr3g0467971 RchiOBHm_Chr4g0400901 RchiOBHm_Chr6g0248011 RchiOBHm_Chr7g0232381 RchiOBHm_Chr7g0238121
rosa_laevigata RLG00000001350 RLG00000009167 RLG00000015163 RLG00000017708 RLG00000017741 RLG00000021807 RLG00000023849 RLG00000026518
rosa_multiflora Rmu_sc0000332.1_g000021 Rmu_sc0000332.1_g000029 Rmu_sc0002416.1_g000007 Rmu_sc0002611.1_g000001 Rmu_sc0003113.1_g000016 Rmu_sc0004780.1_g000001 Rmu_sc0004805.1_g000035 Rmu_sc0010633.1_g000010 Rmu_sc0011069.1_g000003 Rmu_sc0016170.1_g000002 Rmu_sc0025757.1_g000003 Rmu_ssc0000484.1_g000003
rosa_roxburghii Rroxscaffold_2G00134200 Rroxscaffold_4G00321030 Rroxscaffold_7G00213500
rosa_rugosa Rorug01G0283100 Rorug02G0160500 Rorug02G0535300 Rorug04G0027000 Rorug04G0328100 Rorug05G0388700 Rorug05G0530200 Rorug06G0489200 Rorug07G0104900 Rorug07G0209400 Rorug07G0274500
rosa_samantha Rh2BG222800 Rh2BG618900 Rh2CG214400 Rh2CG587200 Rh6BG040200 Rh6CG206500 Rh6DG035600 Rh7BG401600 Rh7BG401700 Rh7DG418400
rosa_wichuraiana Rw2G050240 Rw6G003920 Rw7G035430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 122
AccB1I GGYRCC 3 cut(s) 170, 778, 1038
AccI GTMKAC 1 cut(s) 477
AccII CGCG 1 cut(s) 192
AccIII TCCGGA 1 cut(s) 366
AciI CCGC 7 cut(s) 129, 132, 190, 282, 297, 377, 989
AclWI GGATC 7 cut(s) 213, 354, 358, 371, 445, 457, 911
AcoI YGGCCR 5 cut(s) 28, 100, 906, 996, 1104
AcuI CTGAAG 2 cut(s) 142, 1341
AcyI GRCGYC 3 cut(s) 141, 171, 294
AfaI GTAC 7 cut(s) 388, 515, 935, 956, 972, 1089, 1349
AfiI CCNNNNNNNGG 1 cut(s) 926
AgsI TTSAA 4 cut(s) 148, 398, 1054, 1167
AjiI CACGTC 1 cut(s) 184
AjnI CCWGG 1 cut(s) 1090
AleI CACNNNNGTG 1 cut(s) 596
AloI GAACNNNNNNTCC 2 cut(s) 1235, 1267
AluBI AGCT 6 cut(s) 67, 256, 274, 373, 499, 1262
AluI AGCT 6 cut(s) 67, 256, 274, 373, 499, 1262
Alw21I GWGCWC 3 cut(s) 591, 742, 1218
Alw26I GTCTC 3 cut(s) 129, 535, 655
Alw44I GTGCAC 1 cut(s) 1214
AlwI GGATC 7 cut(s) 213, 354, 358, 371, 445, 457, 911
Aor13HI TCCGGA 1 cut(s) 366
AoxI GGCC 7 cut(s) 28, 100, 316, 840, 906, 996, 1104
ApaLI GTGCAC 1 cut(s) 1214
ApeKI GCWGC 2 cut(s) 271, 1277
AspA2I CCTAGG 1 cut(s) 920
AspLEI GCGC 3 cut(s) 173, 194, 884
AspS9I GGNCC 2 cut(s) 237, 1017
AsuHPI GGTGA 7 cut(s) 244, 865, 1109, 1241, 1259, 1337, 1346
AvaII GGWCC 2 cut(s) 237, 1017
AvrII CCTAGG 1 cut(s) 920
AxyI CCTNAGG 1 cut(s) 525
BaeGI GKGCMC 2 cut(s) 1043, 1218
BaeI ACNNNNGTAYC 2 cut(s) 1071, 1104
BalI TGGCCA 4 cut(s) 102, 908, 998, 1106
BamHI GGATCC 1 cut(s) 363
BanI GGYRCC 3 cut(s) 170, 778, 1038
BanII GRGCYC 1 cut(s) 216
Bbv12I GWGCWC 3 cut(s) 591, 742, 1218
BbvCI CCTCAGC 1 cut(s) 1155
BbvI GCAGC 2 cut(s) 283, 1264
BccI CCATC 7 cut(s) 26, 92, 526, 632, 728, 898, 1325
BceAI ACGGC 3 cut(s) 100, 179, 624
BciT130I CCWGG 1 cut(s) 1092
BclI TGATCA 1 cut(s) 1174
BcoDI GTCTC 3 cut(s) 129, 535, 655
BfaI CTAG 3 cut(s) 921, 1263, 1351
BfoI RGCGCY 2 cut(s) 174, 885
BglI GCCNNNNNGGC 1 cut(s) 839
BisI GCNGC 6 cut(s) 130, 272, 283, 297, 990, 1278
BlnI CCTAGG 1 cut(s) 920
BlsI GCNGC 6 cut(s) 131, 273, 284, 298, 991, 1279
BmcAI AGTACT 1 cut(s) 1349
Bme1390I CCNGG 1 cut(s) 1092
Bme18I GGWCC 2 cut(s) 237, 1017
BmgBI CACGTC 1 cut(s) 184
BmgT120I GGNCC 2 cut(s) 237, 1017
BmiI GGNNCC 6 cut(s) 172, 213, 365, 549, 780, 1040
BmrFI CCNGG 1 cut(s) 1092
BmrI ACTGGG 1 cut(s) 399
BmsI GCATC 1 cut(s) 21
BmuI ACTGGG 1 cut(s) 399
BplI GAGNNNNNCTC 7 cut(s) 31, 455, 487, 842, 874, 881, 913
BpmI CTGGAG 1 cut(s) 230
Bpu10I CCTNAGC 2 cut(s) 267, 1155
BpuEI CTTGAG 1 cut(s) 1304
BsaHI GRCGYC 3 cut(s) 141, 171, 294
BsaI GGTCTC 2 cut(s) 535, 655
BsaJI CCNNGG 2 cut(s) 920, 1107
BsaWI WCCGGW 2 cut(s) 239, 366
BsaXI ACNNNNNCTCC 4 cut(s) 954, 984, 1235, 1265
Bsc4I CCNNNNNNNGG 1 cut(s) 926
Bse118I RCCGGY 1 cut(s) 167
Bse1I ACTGG 5 cut(s) 394, 439, 821, 1051, 1223
Bse21I CCTNAGG 1 cut(s) 525
BseAI TCCGGA 1 cut(s) 366
BseBI CCWGG 1 cut(s) 1092
BseDI CCNNGG 2 cut(s) 920, 1107
BseGI GGATG 5 cut(s) 18, 757, 809, 985, 1345
BseLI CCNNNNNNNGG 1 cut(s) 926
BseMII CTCAG 6 cut(s) 258, 539, 710, 750, 1146, 1269
BseNI ACTGG 5 cut(s) 394, 439, 821, 1051, 1223
BseRI GAGGAG 3 cut(s) 513, 975, 1256
BseSI GKGCMC 2 cut(s) 1043, 1218
BseXI GCAGC 2 cut(s) 283, 1264
BseYI CCCAGC 1 cut(s) 60
Bsh1236I CGCG 1 cut(s) 192
BshFI GGCC 7 cut(s) 30, 102, 318, 842, 908, 998, 1106
BshNI GGYRCC 3 cut(s) 170, 778, 1038
BsiHKAI GWGCWC 3 cut(s) 591, 742, 1218
BsiSI CCGG 5 cut(s) 36, 168, 174, 240, 367
BslFI GGGAC 2 cut(s) 170, 445
BslI CCNNNNNNNGG 1 cut(s) 926
BsmAI GTCTC 3 cut(s) 129, 535, 655
BsmBI CGTCTC 1 cut(s) 129
BsmFI GGGAC 2 cut(s) 170, 445
BsmI GAATGC 2 cut(s) 87, 991
BsnI GGCC 7 cut(s) 30, 102, 318, 842, 908, 998, 1106
Bso31I GGTCTC 2 cut(s) 535, 655
Bsp1286I GDGCHC 5 cut(s) 216, 591, 742, 1043, 1218
Bsp13I TCCGGA 1 cut(s) 366
Bsp1407I TGTACA 1 cut(s) 954
Bsp143I GATC 8 cut(s) 205, 346, 363, 437, 449, 916, 1174, 1186
BspACI CCGC 7 cut(s) 129, 132, 190, 282, 297, 377, 989
BspANI GGCC 7 cut(s) 30, 102, 318, 842, 908, 998, 1106
BspCNI CTCAG 6 cut(s) 259, 538, 709, 751, 1147, 1268
BspEI TCCGGA 1 cut(s) 366
BspFNI CGCG 1 cut(s) 192
BspLI GGNNCC 6 cut(s) 172, 213, 365, 549, 780, 1040
BspPI GGATC 7 cut(s) 213, 354, 358, 371, 445, 457, 911
BspQI GCTCTTC 1 cut(s) 263
BspT107I GGYRCC 3 cut(s) 170, 778, 1038
BspTNI GGTCTC 2 cut(s) 535, 655
BsrFI RCCGGY 1 cut(s) 167
BsrGI TGTACA 1 cut(s) 954
BsrI ACTGG 5 cut(s) 394, 439, 821, 1051, 1223
BssAI RCCGGY 1 cut(s) 167
BssECI CCNNGG 2 cut(s) 920, 1107
BssMI GATC 8 cut(s) 205, 346, 363, 437, 449, 916, 1174, 1186
BssNI GRCGYC 3 cut(s) 141, 171, 294
BssT1I CCWWGG 2 cut(s) 920, 1107
Bst2UI CCWGG 1 cut(s) 1092
Bst4CI ACNGT 7 cut(s) 124, 220, 251, 386, 511, 560, 1084
Bst6I CTCTTC 2 cut(s) 263, 1338
BstACI GRCGYC 3 cut(s) 141, 171, 294
BstAPI GCANNNNNTGC 1 cut(s) 57
BstAUI TGTACA 1 cut(s) 954
BstC8I GCNNGC 5 cut(s) 58, 375, 894, 943, 1206
BstDEI CTNAG 7 cut(s) 267, 495, 525, 696, 759, 1155, 1255
BstF5I GGATG 5 cut(s) 18, 757, 809, 985, 1345
BstFNI CGCG 1 cut(s) 192
BstH2I RGCGCY 2 cut(s) 174, 885
BstHHI GCGC 3 cut(s) 173, 194, 884
BstKTI GATC 8 cut(s) 208, 349, 366, 440, 452, 919, 1177, 1189
BstMAI GTCTC 3 cut(s) 129, 535, 655
BstMBI GATC 8 cut(s) 205, 346, 363, 437, 449, 916, 1174, 1186
BstMWI GCNNNNNNNGC 6 cut(s) 57, 168, 586, 839, 995, 1205
BstNI CCWGG 1 cut(s) 1092
BstSCI CCNGG 1 cut(s) 1090
BstSLI GKGCMC 2 cut(s) 1043, 1218
BstUI CGCG 1 cut(s) 192
BstV1I GCAGC 2 cut(s) 283, 1264
BstX2I RGATCY 5 cut(s) 205, 346, 363, 437, 449
BstYI RGATCY 5 cut(s) 205, 346, 363, 437, 449
Bsu36I CCTNAGG 1 cut(s) 525
BsuRI GGCC 7 cut(s) 30, 102, 318, 842, 908, 998, 1106
BtrI CACGTC 1 cut(s) 184
BtsCI GGATG 5 cut(s) 18, 757, 809, 985, 1345
BtsI GCAGTG 1 cut(s) 714
BtsIMutI CAGTG 4 cut(s) 565, 591, 714, 1216
Cac8I GCNNGC 5 cut(s) 58, 375, 894, 943, 1206
CfoI GCGC 3 cut(s) 173, 194, 884
Cfr10I RCCGGY 1 cut(s) 167
Cfr13I GGNCC 2 cut(s) 237, 1017
CseI GACGC 2 cut(s) 149, 302
CsiI ACCWGGT 1 cut(s) 1090
Csp6I GTAC 7 cut(s) 387, 514, 934, 955, 971, 1088, 1348
CspCI CAANNNNNGTGG 2 cut(s) 1132, 1167
CviAII CATG 5 cut(s) 802, 848, 926, 937, 1129
CviQI GTAC 7 cut(s) 387, 514, 934, 955, 971, 1088, 1348
DdeI CTNAG 7 cut(s) 267, 495, 525, 696, 759, 1155, 1255
DinI GGCGCC 1 cut(s) 172
DpnI GATC 8 cut(s) 207, 348, 365, 439, 451, 918, 1176, 1188
DpnII GATC 8 cut(s) 205, 346, 363, 437, 449, 916, 1174, 1186
DrdI GACNNNNNNGTC 1 cut(s) 122
DseDI GACNNNNNNGTC 1 cut(s) 122
EaeI YGGCCR 5 cut(s) 28, 100, 906, 996, 1104
Eam1104I CTCTTC 2 cut(s) 263, 1338
EarI CTCTTC 2 cut(s) 263, 1338
EciI GGCGGA 1 cut(s) 147
Eco130I CCWWGG 2 cut(s) 920, 1107
Eco147I AGGCCT 1 cut(s) 842
Eco24I GRGCYC 1 cut(s) 216
Eco31I GGTCTC 2 cut(s) 535, 655
Eco32I GATATC 1 cut(s) 634
Eco47I GGWCC 2 cut(s) 237, 1017
Eco57I CTGAAG 2 cut(s) 142, 1341
Eco81I CCTNAGG 1 cut(s) 525
EcoRII CCWGG 1 cut(s) 1090
EcoRV GATATC 1 cut(s) 634
EcoT14I CCWWGG 2 cut(s) 920, 1107
EcoT38I GRGCYC 1 cut(s) 216
EgeI GGCGCC 1 cut(s) 172
EheI GGCGCC 1 cut(s) 172
ErhI CCWWGG 2 cut(s) 920, 1107
Esp3I CGTCTC 1 cut(s) 129
FaeI CATG 5 cut(s) 805, 851, 929, 940, 1132
FaqI GGGAC 2 cut(s) 170, 445
FatI CATG 5 cut(s) 801, 847, 925, 936, 1128
FauI CCCGC 1 cut(s) 197
FbaI TGATCA 1 cut(s) 1174
FblI GTMKAC 1 cut(s) 477
Fnu4HI GCNGC 6 cut(s) 130, 272, 283, 297, 990, 1278
FokI GGATG 4 cut(s) 5, 764, 796, 992
FriOI GRGCYC 1 cut(s) 216
Fsp4HI GCNGC 6 cut(s) 130, 272, 283, 297, 990, 1278
FspBI CTAG 3 cut(s) 921, 1263, 1351
GlaI GCGC 3 cut(s) 172, 193, 883
GluI GCNGC 6 cut(s) 130, 272, 283, 297, 990, 1278
GsaI CCCAGC 1 cut(s) 64
GsuI CTGGAG 1 cut(s) 230
HaeII RGCGCY 2 cut(s) 174, 885
HaeIII GGCC 7 cut(s) 30, 102, 318, 842, 908, 998, 1106
HapII CCGG 5 cut(s) 36, 168, 174, 240, 367
HgaI GACGC 2 cut(s) 149, 302
HhaI GCGC 3 cut(s) 173, 194, 884
Hin1I GRCGYC 3 cut(s) 141, 171, 294
Hin1II CATG 5 cut(s) 805, 851, 929, 940, 1132
Hin6I GCGC 3 cut(s) 171, 192, 882
HinP1I GCGC 3 cut(s) 171, 192, 882
HincII GTYRAC 1 cut(s) 478
HindII GTYRAC 1 cut(s) 478
HindIII AAGCTT 2 cut(s) 371, 497
HinfI GANTC 3 cut(s) 39, 792, 1313
HpaII CCGG 5 cut(s) 36, 168, 174, 240, 367
HphI GGTGA 7 cut(s) 244, 865, 1109, 1241, 1259, 1337, 1346
Hpy166II GTNNAC 4 cut(s) 478, 514, 570, 1216
Hpy188I TCNGA 4 cut(s) 425, 1116, 1191, 1258
Hpy188III TCNNGA 7 cut(s) 209, 331, 367, 441, 766, 1006, 1283
Hpy8I GTNNAC 4 cut(s) 478, 514, 570, 1216
Hpy99I CGWCG 3 cut(s) 128, 143, 230
HpyAV CCTTC 5 cut(s) 154, 328, 832, 1282, 1316
HpyCH4III ACNGT 7 cut(s) 124, 220, 251, 386, 511, 560, 1084
HpyCH4IV ACGT 1 cut(s) 183
HpyCH4V TGCA 4 cut(s) 12, 818, 1128, 1216
HpyF10VI GCNNNNNNNGC 6 cut(s) 57, 168, 586, 839, 995, 1205
HpyF3I CTNAG 7 cut(s) 267, 495, 525, 696, 759, 1155, 1255
HpySE526I ACGT 1 cut(s) 183
Hsp92I GRCGYC 3 cut(s) 141, 171, 294
Hsp92II CATG 5 cut(s) 805, 851, 929, 940, 1132
HspAI GCGC 3 cut(s) 171, 192, 882
KasI GGCGCC 1 cut(s) 170
Kpn2I TCCGGA 1 cut(s) 366
Ksp22I TGATCA 1 cut(s) 1174
Kzo9I GATC 8 cut(s) 205, 346, 363, 437, 449, 916, 1174, 1186
LguI GCTCTTC 1 cut(s) 263
LmnI GCTCC 5 cut(s) 64, 211, 547, 586, 737
Lsp1109I GCAGC 2 cut(s) 283, 1264
LweI GCATC 1 cut(s) 21
MabI ACCWGGT 1 cut(s) 1090
MaeI CTAG 3 cut(s) 921, 1263, 1351
MaeII ACGT 1 cut(s) 183
MaeIII GTNAC 3 cut(s) 113, 754, 1097
MalI GATC 8 cut(s) 207, 348, 365, 439, 451, 918, 1176, 1188
MboI GATC 8 cut(s) 205, 346, 363, 437, 449, 916, 1174, 1186
MboII GAAGA 5 cut(s) 148, 250, 499, 1310, 1340
MflI RGATCY 5 cut(s) 205, 346, 363, 437, 449
MhlI GDGCHC 5 cut(s) 216, 591, 742, 1043, 1218
MlsI TGGCCA 4 cut(s) 102, 908, 998, 1106
MluNI TGGCCA 4 cut(s) 102, 908, 998, 1106
Mly113I GGCGCC 1 cut(s) 171
MlyI GAGTC 1 cut(s) 1322
MmeI TCCRAC 3 cut(s) 403, 1161, 1290
Mox20I TGGCCA 4 cut(s) 102, 908, 998, 1106
MroI TCCGGA 1 cut(s) 366
MscI TGGCCA 4 cut(s) 102, 908, 998, 1106
MslI CAYNNNNRTG 3 cut(s) 594, 596, 1221
Msp20I TGGCCA 4 cut(s) 102, 908, 998, 1106
MspA1I CMGCKG 1 cut(s) 282
MspI CCGG 5 cut(s) 36, 168, 174, 240, 367
MspR9I CCNGG 1 cut(s) 1092
Mva1269I GAATGC 2 cut(s) 87, 991
MvaI CCWGG 1 cut(s) 1092
MvnI CGCG 1 cut(s) 192
MwoI GCNNNNNNNGC 6 cut(s) 57, 168, 586, 839, 995, 1205
NarI GGCGCC 1 cut(s) 171
NdeII GATC 8 cut(s) 205, 346, 363, 437, 449, 916, 1174, 1186
NlaIII CATG 5 cut(s) 805, 851, 929, 940, 1132
NlaIV GGNNCC 6 cut(s) 172, 213, 365, 549, 780, 1040
NmeAIII GCCGAG 2 cut(s) 135, 859
NmuCI GTSAC 3 cut(s) 113, 754, 1097
OliI CACNNNNGTG 1 cut(s) 596
PceI AGGCCT 1 cut(s) 842
PciSI GCTCTTC 1 cut(s) 263
PctI GAATGC 2 cut(s) 87, 991
PfeI GAWTC 2 cut(s) 39, 792
PkrI GCNGC 6 cut(s) 131, 273, 284, 298, 991, 1279
PleI GAGTC 1 cut(s) 1321
PluTI GGCGCC 1 cut(s) 174
PpsI GAGTC 1 cut(s) 1321
Psp6I CCWGG 1 cut(s) 1090
PspFI CCCAGC 1 cut(s) 60
PspGI CCWGG 1 cut(s) 1090
PspN4I GGNNCC 6 cut(s) 172, 213, 365, 549, 780, 1040
PspPI GGNCC 2 cut(s) 237, 1017
PsuI RGATCY 5 cut(s) 205, 346, 363, 437, 449
RsaI GTAC 7 cut(s) 388, 515, 935, 956, 972, 1089, 1349
RsaNI GTAC 7 cut(s) 387, 514, 934, 955, 971, 1088, 1348
RseI CAYNNNNRTG 3 cut(s) 594, 596, 1221
SalI GTCGAC 1 cut(s) 476
SapI GCTCTTC 1 cut(s) 263
SatI GCNGC 6 cut(s) 130, 272, 283, 297, 990, 1278
Sau3AI GATC 8 cut(s) 205, 346, 363, 437, 449, 916, 1174, 1186
Sau96I GGNCC 2 cut(s) 237, 1017
ScaI AGTACT 1 cut(s) 1349
SchI GAGTC 1 cut(s) 1322
ScrFI CCNGG 1 cut(s) 1092
SduI GDGCHC 5 cut(s) 216, 591, 742, 1043, 1218
SexAI ACCWGGT 1 cut(s) 1090
SfaNI GCATC 1 cut(s) 21
SfoI GGCGCC 1 cut(s) 172
SinI GGWCC 2 cut(s) 237, 1017
SmiMI CAYNNNNRTG 3 cut(s) 594, 596, 1221
SmlI CTYRAG 1 cut(s) 1283
SmoI CTYRAG 1 cut(s) 1283
SseBI AGGCCT 1 cut(s) 842
SsiI CCGC 7 cut(s) 129, 132, 190, 282, 297, 377, 989
SspDI GGCGCC 1 cut(s) 170
SspMI CTAG 3 cut(s) 921, 1263, 1351
StuI AGGCCT 1 cut(s) 842
StyD4I CCNGG 1 cut(s) 1090
StyI CCWWGG 2 cut(s) 920, 1107
TaaI ACNGT 7 cut(s) 124, 220, 251, 386, 511, 560, 1084
TaiI ACGT 1 cut(s) 186
TaqI TCGA 3 cut(s) 78, 225, 477
TatI WGTACW 4 cut(s) 386, 933, 954, 1347
TauI GCSGC 4 cut(s) 132, 285, 299, 992
TfiI GAWTC 2 cut(s) 39, 792
TscAI CASTG 4 cut(s) 565, 598, 721, 1223
TseFI GTSAC 3 cut(s) 113, 754, 1097
TseI GCWGC 2 cut(s) 271, 1277
Tsp45I GTSAC 3 cut(s) 113, 754, 1097
TspDTI ATGAA 4 cut(s) 633, 790, 798, 1311
TspRI CASTG 4 cut(s) 565, 598, 721, 1223
VneI GTGCAC 1 cut(s) 1214
VpaK11BI GGWCC 2 cut(s) 237, 1017
XcmI CCANNNNNNNNNTGG 1 cut(s) 1027
XmaJI CCTAGG 1 cut(s) 920
XmiI GTMKAC 1 cut(s) 477
XspI CTAG 3 cut(s) 921, 1263, 1351
ZrmI AGTACT 1 cut(s) 1349
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.