RchiOBHm_Chr6g0248011

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0000549
Physical Location & Seq
Reverse (-)
4047961 .. 4050838
2878 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ22234

Sequence Viewer

Length: 1353 bp
ATGAGAGAGTGCATTTCGATCCACATCGGTCAGGCCGGTATTCAGGTCGGCAATGCCTGCTGGGAGCTTTATTGCCTTGAGCATGGTATCCAGCCTGATGGCCAAATGCCAAGTGACAAGACTGTAGGCGGAGGAGACGATGCCTTCAACACCTTTTTCAGTGAAACTGGTGCCGGGAAGCATGTCCCTCGCGCAATCTTTGTTGACCTCGAGCCCACAGTTATTGATGAAGTGAGGACTGGAACCTACCGCCAGCTCTTCCACCCTGAGCAGCTCATCAGCGGCAAGGAAGATGCTGCCAACAACTTTGCCAGAGGTCACTATACCATTGGCAAGGAGATTGTTGATCTCTGCTTGGACCGCATCAGGAAACTCGCTGACAACTGCACTGGTCTGCAAGGGTTCCTGGTTTTCAATGCTGTTGGTGGAGGCACTGGCTCCGGGCTTGGATCCCTTCTGTTGGAGCGTTTGTCTGTTGACTATGGAAAGAAGTCAAAGCTTGGGTTCACTGTGTACCCCTCTCCACAGGTTTCTACCTCTGTGGTTGAGCCCTACAACAGTGTACTCTCAACCCACTCCCTCCTGGAGCACACAGATGTTGCTGTTCTCCTTGACAATGAGGCAATCTATGATATCTGCAGGCGCTCTCTTGACATTGAGCGACCCACCTACACAAACCTCAACCGACTTGTATCTCAGGTCATTTCCTCTTTGACTGCCTCCTTGAGGTTTGACGGTGCTCTCAATGTGGATGTTACTGAGTTCCAGACCAACTTGGTTCCCTACCCCAGAATCCACTTTATGCTTTCTTCCTATGCACCTGTCATCTCTGCTGAGAAGGCTTACCATGAACAGCTCTCAGTGGCTGAGATCACCAACAGTGCTTTTGAACCCTCATCTATGATGGCCAAGTGCGATCCTCGCCATGGGAAATACATGGCTTGCTGTTTGATGTACCGTGGTGATGTTGTACCCAAGGACGTGAATGCTGCTGTTGCCACCATCAAGACCAAGCGCACAATTCAATTTGTTGACTGGTGCCCTACCGGATTCAAGTGCGGTATCAACTACCAGCCACCCACTGTTGTCCCCGGAGGTGACCTTGCCAAGGTGCAGAGGGCTGTCTGCATGATCTCCAACTCAACCAGTGTTGCTGAGGTCTTCTCAAGAATTGACCACAAGTTTGATCTCATGTATGCCAAGCGTGCTTTCGTGCACTGGTATGTGGGTGAGGGTATGGAGGAAGGTGAGTTCTCTGAGGCCCGTGAGGATCTTGCTGCCCTCGAGAAGGATTATGAAGAGGTTGGTGCTGAGTCTGCTGAGGGCGATGATGATGAAGGGGATGAGTACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04145 ko04210 ko04530 ko04540 ko05130 map04145 map04210 map04530 map04540 map05130
Pfam Domains
Protein Families
Tubulin Tubulin_C

Protein Analysis

450

Amino Acids

49.57

Weight (kDa)

4.92

Isoelectric Point (pI)

36.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tubulin PF00091 3 - 213 9.4e-63 Tubulin/FtsZ family, GTPase domain
Tubulin_C PF03953 263 - 392 4.5e-52 Tubulin C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000549)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G04820 AT1G50010 AT4G14960 AT4G14960
fragaria_vesca FvH4_1g18660 FvH4_2g03140 FvH4_5g33560 FvH4_6g48940 FvH4_6g48940
malus_domestica MD02G1204800.v1.1 MD05G1106800.v1.1 MD08G1210600.v1.1 MD09G1049500.v1.1 MD10G1111600.v1.1 MD15G1398700.v1.1 MD17G1049700.v1.1
prunus_persica Prupe.1G542600_v2.0.a1 Prupe.3G269300_v2.0.a1 Prupe.6G212700_v2.0.a1 Prupe.8G150900_v2.0.a1
pyrus_communis pycom05g10370 pycom08g18150 pycom10g09640 pycom10g09660 pycom111g04050 pycom15g35470 pycom17g04460
rosa_chinensis RchiOBHm_Chr2g0109481 RchiOBHm_Chr2g0168751 RchiOBHm_Chr3g0453071 RchiOBHm_Chr3g0460741 RchiOBHm_Chr3g0467971 RchiOBHm_Chr4g0400901 RchiOBHm_Chr6g0248011 RchiOBHm_Chr7g0232381 RchiOBHm_Chr7g0238121
rosa_laevigata RLG00000001350 RLG00000009167 RLG00000015163 RLG00000017708 RLG00000017741 RLG00000021807 RLG00000023849 RLG00000026518
rosa_multiflora Rmu_sc0000332.1_g000021 Rmu_sc0000332.1_g000029 Rmu_sc0002416.1_g000007 Rmu_sc0002611.1_g000001 Rmu_sc0003113.1_g000016 Rmu_sc0004780.1_g000001 Rmu_sc0004805.1_g000035 Rmu_sc0010633.1_g000010 Rmu_sc0011069.1_g000003 Rmu_sc0016170.1_g000002 Rmu_sc0025757.1_g000003 Rmu_ssc0000484.1_g000003
rosa_roxburghii Rroxscaffold_2G00134200 Rroxscaffold_4G00321030 Rroxscaffold_7G00213500
rosa_rugosa Rorug01G0283100 Rorug02G0160500 Rorug02G0535300 Rorug04G0027000 Rorug04G0328100 Rorug05G0388700 Rorug05G0530200 Rorug06G0489200 Rorug07G0104900 Rorug07G0209400 Rorug07G0274500
rosa_samantha Rh2BG222800 Rh2BG618900 Rh2CG214400 Rh2CG587200 Rh6BG040200 Rh6CG206500 Rh6DG035600 Rh7BG401600 Rh7BG401700 Rh7DG418400
rosa_wichuraiana Rw2G050240 Rw6G003920 Rw7G035430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 170, 1038
AccII CGCG 1 cut(s) 192
AciI CCGC 5 cut(s) 129, 250, 282, 361, 1059
AclWI GGATC 5 cut(s) 13, 444, 457, 911, 1278
AcoI YGGCCR 2 cut(s) 100, 906
AfaI GTAC 5 cut(s) 515, 564, 956, 972, 1349
AfiI CCNNNNNNNGG 5 cut(s) 460, 726, 926, 1108, 1288
AgsI TTSAA 5 cut(s) 148, 415, 890, 1025, 1054
AjiI CACGTC 1 cut(s) 982
AjnI CCWGG 2 cut(s) 405, 582
AjuI GAANNNNNNNTTGG 2 cut(s) 1193, 1225
AloI GAACNNNNNNTCC 2 cut(s) 1235, 1267
AluBI AGCT 5 cut(s) 67, 256, 274, 499, 856
AluI AGCT 5 cut(s) 67, 256, 274, 499, 856
Alw21I GWGCWC 3 cut(s) 591, 742, 1218
Alw26I GTCTC 1 cut(s) 129
Alw44I GTGCAC 1 cut(s) 1214
AlwI GGATC 5 cut(s) 13, 444, 457, 911, 1278
AlwNI CAGNNNCTG 1 cut(s) 866
Ama87I CYCGRG 2 cut(s) 209, 1283
AoxI GGCC 4 cut(s) 33, 100, 906, 1260
ApaLI GTGCAC 1 cut(s) 1214
ApeKI GCWGC 4 cut(s) 271, 296, 989, 1277
ArsI GACNNNNNNTTYG 2 cut(s) 1166, 1198
AspLEI GCGC 3 cut(s) 194, 645, 1017
AspS9I GGNCC 2 cut(s) 358, 1261
AsuC2I CCSGG 3 cut(s) 175, 442, 1092
AsuHPI GGTGA 5 cut(s) 865, 974, 1109, 1241, 1259
AvaI CYCGRG 2 cut(s) 209, 1283
AvaII GGWCC 1 cut(s) 358
BaeGI GKGCMC 2 cut(s) 1043, 1218
BalI TGGCCA 2 cut(s) 102, 908
BamHI GGATCC 1 cut(s) 449
BanI GGYRCC 2 cut(s) 170, 1038
BanII GRGCYC 2 cut(s) 216, 552
BbsI GAAGAC 1 cut(s) 1153
Bbv12I GWGCWC 3 cut(s) 591, 742, 1218
BbvCI CCTCAGC 2 cut(s) 1155, 1320
BbvI GCAGC 4 cut(s) 283, 283, 976, 1264
BccI CCATC 3 cut(s) 92, 898, 1010
BcgI CGANNNNNNTGC 2 cut(s) 170, 204
BciT130I CCWGG 2 cut(s) 407, 584
BciVI GTATCC 1 cut(s) 98
BcnI CCSGG 3 cut(s) 175, 442, 1092
BcoDI GTCTC 1 cut(s) 129
BfmI CTRYAG 2 cut(s) 123, 637
BfoI RGCGCY 1 cut(s) 646
BfuI GTATCC 1 cut(s) 98
BisI GCNGC 5 cut(s) 272, 283, 297, 990, 1278
BlsI GCNGC 5 cut(s) 273, 284, 298, 991, 1279
BmcAI AGTACT 1 cut(s) 1349
Bme1390I CCNGG 5 cut(s) 175, 407, 442, 584, 1092
Bme18I GGWCC 1 cut(s) 358
BmeT110I CYCGRG 2 cut(s) 209, 1283
BmgBI CACGTC 1 cut(s) 982
BmgT120I GGNCC 2 cut(s) 358, 1261
BmiI GGNNCC 7 cut(s) 172, 244, 404, 439, 451, 780, 1040
BmrFI CCNGG 5 cut(s) 175, 407, 442, 584, 1092
BmsI GCATC 3 cut(s) 130, 283, 372
BpiI GAAGAC 1 cut(s) 1153
BplI GAGNNNNNCTC 2 cut(s) 1148, 1180
BpmI CTGGAG 1 cut(s) 605
Bpu10I CCTNAGC 3 cut(s) 267, 1155, 1320
BpuEI CTTGAG 3 cut(s) 98, 745, 1150
BpuMI CCSGG 3 cut(s) 175, 442, 1092
BsaBI GATNNNNATC 1 cut(s) 23
BsaJI CCNNGG 5 cut(s) 925, 958, 975, 1090, 1107
BsaWI WCCGGW 1 cut(s) 1046
BsaXI ACNNNNNCTCC 2 cut(s) 455, 485
Bsc4I CCNNNNNNNGG 5 cut(s) 460, 726, 926, 1108, 1288
Bse118I RCCGGY 1 cut(s) 35
Bse1I ACTGG 7 cut(s) 172, 244, 394, 439, 1040, 1146, 1223
Bse3DI GCAATG 1 cut(s) 58
Bse8I GATNNNNATC 1 cut(s) 23
BseBI CCWGG 2 cut(s) 407, 584
BseDI CCNNGG 5 cut(s) 925, 958, 975, 1090, 1107
BseGI GGATG 2 cut(s) 757, 1348
BseJI GATNNNNATC 1 cut(s) 23
BseLI CCNNNNNNNGG 5 cut(s) 460, 726, 926, 1108, 1288
BseMI GCAATG 1 cut(s) 58
BseNI ACTGG 7 cut(s) 172, 244, 394, 439, 1040, 1146, 1223
BseRI GAGGAG 1 cut(s) 147
BseSI GKGCMC 2 cut(s) 1043, 1218
BseXI GCAGC 4 cut(s) 283, 283, 976, 1264
BseYI CCCAGC 1 cut(s) 60
BsgI GTGCAG 2 cut(s) 370, 1133
Bsh1236I CGCG 1 cut(s) 192
BshFI GGCC 4 cut(s) 35, 102, 908, 1262
BshNI GGYRCC 2 cut(s) 170, 1038
BsiHKAI GWGCWC 3 cut(s) 591, 742, 1218
BsiHKCI CYCGRG 2 cut(s) 209, 1283
BsiSI CCGG 5 cut(s) 36, 174, 441, 1047, 1092
BslFI GGGAC 2 cut(s) 170, 1073
BslI CCNNNNNNNGG 5 cut(s) 460, 726, 926, 1108, 1288
BsmAI GTCTC 1 cut(s) 129
BsmBI CGTCTC 1 cut(s) 129
BsmFI GGGAC 2 cut(s) 170, 1073
BsmI GAATGC 1 cut(s) 991
BsnI GGCC 4 cut(s) 35, 102, 908, 1262
BsoBI CYCGRG 2 cut(s) 209, 1283
Bsp1286I GDGCHC 6 cut(s) 216, 552, 591, 742, 1043, 1218
Bsp143I GATC 8 cut(s) 18, 346, 449, 870, 916, 1131, 1186, 1270
Bsp19I CCATGG 1 cut(s) 925
BspACI CCGC 5 cut(s) 129, 250, 282, 361, 1059
BspANI GGCC 4 cut(s) 35, 102, 908, 1262
BspFNI CGCG 1 cut(s) 192
BspLI GGNNCC 7 cut(s) 172, 244, 404, 439, 451, 780, 1040
BspMAI CTGCAG 1 cut(s) 641
BspPI GGATC 5 cut(s) 13, 444, 457, 911, 1278
BspQI GCTCTTC 1 cut(s) 263
BspT107I GGYRCC 2 cut(s) 170, 1038
BsrDI GCAATG 1 cut(s) 58
BsrFI RCCGGY 1 cut(s) 35
BsrI ACTGG 7 cut(s) 172, 244, 394, 439, 1040, 1146, 1223
BssAI RCCGGY 1 cut(s) 35
BssECI CCNNGG 5 cut(s) 925, 958, 975, 1090, 1107
BssMI GATC 8 cut(s) 18, 346, 449, 870, 916, 1131, 1186, 1270
BssT1I CCWWGG 3 cut(s) 925, 975, 1107
Bst2UI CCWGG 2 cut(s) 407, 584
Bst4CI ACNGT 8 cut(s) 124, 220, 511, 560, 737, 881, 959, 1084
Bst6I CTCTTC 2 cut(s) 263, 1293
BstAPI GCANNNNNTGC 1 cut(s) 57
BstC8I GCNNGC 5 cut(s) 58, 254, 641, 943, 1206
BstDSI CCRYGG 2 cut(s) 925, 958
BstEII GGTNACC 1 cut(s) 1097
BstENI CCTNNNNNAGG 3 cut(s) 724, 1106, 1286
BstF5I GGATG 2 cut(s) 757, 1348
BstFNI CGCG 1 cut(s) 192
BstH2I RGCGCY 1 cut(s) 646
BstHHI GCGC 3 cut(s) 194, 645, 1017
BstKTI GATC 8 cut(s) 21, 349, 452, 873, 919, 1134, 1189, 1273
BstMAI GTCTC 1 cut(s) 129
BstMBI GATC 8 cut(s) 18, 346, 449, 870, 916, 1131, 1186, 1270
BstMWI GCNNNNNNNGC 7 cut(s) 57, 360, 839, 921, 995, 1205, 1316
BstNI CCWGG 2 cut(s) 407, 584
BstNSI RCATGY 1 cut(s) 185
BstPI GGTNACC 1 cut(s) 1097
BstSCI CCNGG 5 cut(s) 173, 405, 440, 582, 1090
BstSFI CTRYAG 2 cut(s) 123, 637
BstSLI GKGCMC 2 cut(s) 1043, 1218
BstUI CGCG 1 cut(s) 192
BstV1I GCAGC 4 cut(s) 283, 283, 976, 1264
BstV2I GAAGAC 1 cut(s) 1153
BstX2I RGATCY 2 cut(s) 449, 1270
BstXI CCANNNNNNTGG 1 cut(s) 98
BstYI RGATCY 2 cut(s) 449, 1270
BsuI GTATCC 1 cut(s) 98
BsuRI GGCC 4 cut(s) 35, 102, 908, 1262
BtgI CCRYGG 2 cut(s) 925, 958
BtgZI GCGATG 1 cut(s) 1341
BtrI CACGTC 1 cut(s) 982
BtsCI GGATG 2 cut(s) 757, 1348
Cac8I GCNNGC 5 cut(s) 58, 254, 641, 943, 1206
CaiI CAGNNNCTG 1 cut(s) 866
CfoI GCGC 3 cut(s) 194, 645, 1017
Cfr10I RCCGGY 1 cut(s) 35
Cfr13I GGNCC 2 cut(s) 358, 1261
Csp6I GTAC 5 cut(s) 514, 563, 955, 971, 1348
CspCI CAANNNNNGTGG 2 cut(s) 205, 240
CviAII CATG 7 cut(s) 83, 182, 848, 926, 937, 1129, 1192
CviQI GTAC 5 cut(s) 514, 563, 955, 971, 1348
DpnI GATC 8 cut(s) 20, 348, 451, 872, 918, 1133, 1188, 1272
DpnII GATC 8 cut(s) 18, 346, 449, 870, 916, 1131, 1186, 1270
EaeI YGGCCR 2 cut(s) 100, 906
Eam1104I CTCTTC 2 cut(s) 263, 1293
EarI CTCTTC 2 cut(s) 263, 1293
EciI GGCGGA 1 cut(s) 144
Eco130I CCWWGG 3 cut(s) 925, 975, 1107
Eco24I GRGCYC 2 cut(s) 216, 552
Eco32I GATATC 1 cut(s) 634
Eco47I GGWCC 1 cut(s) 358
Eco88I CYCGRG 2 cut(s) 209, 1283
Eco91I GGTNACC 1 cut(s) 1097
EcoNI CCTNNNNNAGG 3 cut(s) 724, 1106, 1286
EcoO65I GGTNACC 1 cut(s) 1097
EcoRII CCWGG 2 cut(s) 405, 582
EcoRV GATATC 1 cut(s) 634
EcoT14I CCWWGG 3 cut(s) 925, 975, 1107
EcoT38I GRGCYC 2 cut(s) 216, 552
ErhI CCWWGG 3 cut(s) 925, 975, 1107
Esp3I CGTCTC 1 cut(s) 129
FaeI CATG 7 cut(s) 86, 185, 851, 929, 940, 1132, 1195
FaqI GGGAC 2 cut(s) 170, 1073
FatI CATG 7 cut(s) 82, 181, 847, 925, 936, 1128, 1191
Fnu4HI GCNGC 5 cut(s) 272, 283, 297, 990, 1278
FokI GGATG 1 cut(s) 764
FriOI GRGCYC 2 cut(s) 216, 552
Fsp4HI GCNGC 5 cut(s) 272, 283, 297, 990, 1278
GlaI GCGC 3 cut(s) 193, 644, 1016
GluI GCNGC 5 cut(s) 272, 283, 297, 990, 1278
GsaI CCCAGC 1 cut(s) 64
GsuI CTGGAG 1 cut(s) 605
HaeII RGCGCY 1 cut(s) 646
HaeIII GGCC 4 cut(s) 35, 102, 908, 1262
HapII CCGG 5 cut(s) 36, 174, 441, 1047, 1092
HhaI GCGC 3 cut(s) 194, 645, 1017
Hin1II CATG 7 cut(s) 86, 185, 851, 929, 940, 1132, 1195
Hin6I GCGC 3 cut(s) 192, 643, 1015
HinP1I GCGC 3 cut(s) 192, 643, 1015
HincII GTYRAC 3 cut(s) 205, 478, 1033
HindII GTYRAC 3 cut(s) 205, 478, 1033
HindIII AAGCTT 1 cut(s) 497
HinfI GANTC 3 cut(s) 792, 1050, 1313
HpaII CCGG 5 cut(s) 36, 174, 441, 1047, 1092
HphI GGTGA 5 cut(s) 865, 974, 1109, 1241, 1259
Hpy166II GTNNAC 7 cut(s) 205, 478, 507, 514, 563, 1033, 1216
Hpy188I TCNGA 1 cut(s) 1258
Hpy188III TCNNGA 6 cut(s) 367, 650, 766, 1006, 1167, 1285
Hpy8I GTNNAC 7 cut(s) 205, 478, 507, 514, 563, 1033, 1216
HpyAV CCTTC 6 cut(s) 154, 464, 832, 1238, 1282, 1331
HpyCH4III ACNGT 8 cut(s) 124, 220, 511, 560, 737, 881, 959, 1084
HpyCH4IV ACGT 1 cut(s) 981
HpyCH4V TGCA 8 cut(s) 12, 387, 397, 639, 818, 1114, 1128, 1216
HpyF10VI GCNNNNNNNGC 7 cut(s) 57, 360, 839, 921, 995, 1205, 1316
HpySE526I ACGT 1 cut(s) 981
Hsp92II CATG 7 cut(s) 86, 185, 851, 929, 940, 1132, 1195
HspAI GCGC 3 cut(s) 192, 643, 1015
Kzo9I GATC 8 cut(s) 18, 346, 449, 870, 916, 1131, 1186, 1270
LguI GCTCTTC 1 cut(s) 263
LmnI GCTCC 4 cut(s) 64, 443, 463, 586
Lsp1109I GCAGC 4 cut(s) 283, 283, 976, 1264
LweI GCATC 3 cut(s) 130, 283, 372
MaeII ACGT 1 cut(s) 981
MaeIII GTNAC 4 cut(s) 113, 317, 754, 1097
MalI GATC 8 cut(s) 20, 348, 451, 872, 918, 1133, 1188, 1272
MboI GATC 8 cut(s) 18, 346, 449, 870, 916, 1131, 1186, 1270
MboII GAAGA 5 cut(s) 250, 302, 801, 1153, 1310
MflI RGATCY 2 cut(s) 449, 1270
MhlI GDGCHC 6 cut(s) 216, 552, 591, 742, 1043, 1218
MlsI TGGCCA 2 cut(s) 102, 908
MluCI AATT 3 cut(s) 1020, 1025, 1170
MluNI TGGCCA 2 cut(s) 102, 908
MlyI GAGTC 1 cut(s) 1322
MmeI TCCRAC 2 cut(s) 441, 1161
Mox20I TGGCCA 2 cut(s) 102, 908
MscI TGGCCA 2 cut(s) 102, 908
MslI CAYNNNNRTG 2 cut(s) 594, 1221
Msp20I TGGCCA 2 cut(s) 102, 908
MspA1I CMGCKG 1 cut(s) 282
MspI CCGG 5 cut(s) 36, 174, 441, 1047, 1092
MspR9I CCNGG 5 cut(s) 175, 407, 442, 584, 1092
Mva1269I GAATGC 1 cut(s) 991
MvaI CCWGG 2 cut(s) 407, 584
MvnI CGCG 1 cut(s) 192
MwoI GCNNNNNNNGC 7 cut(s) 57, 360, 839, 921, 995, 1205, 1316
NciI CCSGG 3 cut(s) 175, 442, 1092
NcoI CCATGG 1 cut(s) 925
NdeII GATC 8 cut(s) 18, 346, 449, 870, 916, 1131, 1186, 1270
NlaIII CATG 7 cut(s) 86, 185, 851, 929, 940, 1132, 1195
NlaIV GGNNCC 7 cut(s) 172, 244, 404, 439, 451, 780, 1040
NmuCI GTSAC 3 cut(s) 113, 317, 1097
NspI RCATGY 1 cut(s) 185
PaeR7I CTCGAG 2 cut(s) 209, 1283
PciSI GCTCTTC 1 cut(s) 263
PctI GAATGC 1 cut(s) 991
PfeI GAWTC 2 cut(s) 792, 1050
PfoI TCCNGGA 1 cut(s) 582
PkrI GCNGC 5 cut(s) 273, 284, 298, 991, 1279
PleI GAGTC 1 cut(s) 1321
PpsI GAGTC 1 cut(s) 1321
Psp6I CCWGG 2 cut(s) 405, 582
PspEI GGTNACC 1 cut(s) 1097
PspFI CCCAGC 1 cut(s) 60
PspGI CCWGG 2 cut(s) 405, 582
PspN4I GGNNCC 7 cut(s) 172, 244, 404, 439, 451, 780, 1040
PspPI GGNCC 2 cut(s) 358, 1261
PspXI VCTCGAGB 1 cut(s) 209
PstI CTGCAG 1 cut(s) 641
PstNI CAGNNNCTG 1 cut(s) 866
PsuI RGATCY 2 cut(s) 449, 1270
RsaI GTAC 5 cut(s) 515, 564, 956, 972, 1349
RsaNI GTAC 5 cut(s) 514, 563, 955, 971, 1348
RseI CAYNNNNRTG 2 cut(s) 594, 1221
SapI GCTCTTC 1 cut(s) 263
SatI GCNGC 5 cut(s) 272, 283, 297, 990, 1278
Sau3AI GATC 8 cut(s) 18, 346, 449, 870, 916, 1131, 1186, 1270
Sau96I GGNCC 2 cut(s) 358, 1261
ScaI AGTACT 1 cut(s) 1349
SchI GAGTC 1 cut(s) 1322
ScrFI CCNGG 5 cut(s) 175, 407, 442, 584, 1092
SduI GDGCHC 6 cut(s) 216, 552, 591, 742, 1043, 1218
SfaNI GCATC 3 cut(s) 130, 283, 372
SfcI CTRYAG 2 cut(s) 123, 637
Sfr274I CTCGAG 2 cut(s) 209, 1283
SinI GGWCC 1 cut(s) 358
SlaI CTCGAG 2 cut(s) 209, 1283
SmiMI CAYNNNNRTG 2 cut(s) 594, 1221
SmlI CTYRAG 5 cut(s) 77, 209, 724, 1165, 1283
SmoI CTYRAG 5 cut(s) 77, 209, 724, 1165, 1283
Sse9I AATT 3 cut(s) 1020, 1025, 1170
SsiI CCGC 5 cut(s) 129, 250, 282, 361, 1059
StyD4I CCNGG 5 cut(s) 173, 405, 440, 582, 1090
StyI CCWWGG 3 cut(s) 925, 975, 1107
TaaI ACNGT 8 cut(s) 124, 220, 511, 560, 737, 881, 959, 1084
TaiI ACGT 1 cut(s) 984
TaqI TCGA 3 cut(s) 17, 210, 1284
TaqII GACCGA 1 cut(s) 17
TasI AATT 3 cut(s) 1020, 1025, 1170
TatI WGTACW 2 cut(s) 562, 1347
TauI GCSGC 1 cut(s) 285
TfiI GAWTC 2 cut(s) 792, 1050
TseFI GTSAC 3 cut(s) 113, 317, 1097
TseI GCWGC 4 cut(s) 271, 296, 989, 1277
Tsp45I GTSAC 3 cut(s) 113, 317, 1097
TspDTI ATGAA 4 cut(s) 243, 864, 1311, 1350
VneI GTGCAC 1 cut(s) 1214
VpaK11BI GGWCC 1 cut(s) 358
XagI CCTNNNNNAGG 3 cut(s) 724, 1106, 1286
XceI RCATGY 1 cut(s) 185
XhoI CTCGAG 2 cut(s) 209, 1283
ZrmI AGTACT 1 cut(s) 1349
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.