Rorug04G0027000

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0000549
Physical Location & Seq
Forward (+)
3701436 .. 3723511
22076 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0027000.1

Sequence Viewer

Length: 3441 bp
ATGGAGCAAAGCAAGCAGAATATGGCTTCTCTGACCAACGAAGCATCAGCCAGTTCTTCATCTTCTTCTCTCCCACTTTCTTCTACTGATCACCAAAATCATTACACATACGAGGTCTTCCTGAGTTTTCGAGGCGAGGATACACGCTTTAATTTTACAGATCATTTGTATACTGCTCTGTGTCAGAGGGGAATTGAGACCTTCAGAGATGATAAGCTTAGCAGAGGAGAAGACATATCACAAGAGCTCCTCAAAGCAATTGAGGAGTCCAGAGTTTCAATCGTCGTCTTCTCTCAAAATTATGCTTCCTCAAGGTGGTGCTTAGATGAACTGGTCAAGATACTTGACTGCAGAAAATCCAAAGGACAGAAGTTTAGAGCTGTTTTCTACAAGGTTGCTCCCTCAGATGTACGACACCAAACAGGTGCTTTCGGTGACGCTTTTGCTAAAATTGATCAATGCAAATACAAGGATAGCATCGGCAAATGGAGGAAGGCTCTTGAGGAATCAGCAAATTTGTCTGGATGGACTTTCGAGGAGGGCCGGTACGAGACTGAATTTATCAACGACATTGTTGGGGAGTTGTCTGCCCGAGTAGTAAACCCTTCATGTGAGTGGCACGTTGCTCAATATCCAATTGGATTAGAGTCTTGTATACAAGATGTCGACAGTCTTTTAAAAACCGAGGAAAATATTTTTCGCATGGTGGGCATATGGGGGACTGGTGGAATAGGAAAGACCACAATTGCGAAAGCTGTGTTTAATTCAATTTGCCATAAGTTTGAAACTAATTGTTTCTTGGCAGATGTTAGGTCTCATTCAAATGGCCTAGCCCAACTGCAAGAGAGACTTTTGCATGATGTTTTAAGGGACTCAAATTTGAAGGTGAGCAGTGTTGACCAAGGAGTCAGTTTTATAAAGGAAAGAATGCAAAATAAAAAAGTTCTCTTAATCCTTGATGACGTGAGTCATTCTAGCCAATTAAAGAACTTAGTTCCATCCGTTGATTGTTTTGGCCCGGGCAGTAGAATTCTCATAACAACAAGAGATAAACGTTGGCTAATTGCTCATGAAGTTAATGAAGTATATGAGGTCAAGATGTTAGATGATCGTCATGCTTTGGAGTTGTTTAGCTTGCATGCATTCAAAAGAAATGAACCTCCAAGCGATTATCTGAAACTTGCACAACATGTTATACGCTATGCCCAAGGCCTTCCATTAGCTGTGATAGTCTTAGGCTCTCATCTATTTCGTAGAAGCACAGAGGAGTGGGAAGCTACAATAGGTAGTTGTAAAGGAGGTCCTCAGGCAGATATACATAAAGTTCTCAAACTAAGTTATGATGCTCTAGGACGAGATTTAAAAGAACTATTTCTTGATATCGCTTGCTTCTTTAAAGGTAAGCTTGTAGACCGTGTGAAATCAATACTAGAAGCTTGCTACGACCGCAAAACTATGATGATTGGTATTACACAATTGCAAGAAAAAGCCTTGATAAGAATTGACAAAGATGATCAGATTTGGATGCATGACTTGATAGAAGAAATGGGTAAAGACATAGTGTATCAGGAGTCACCTGATGAACCCGGGAAACGCAGCAGAGTGTGGAGTAAAGAAGATGTAGACTACATTCTAACAAATAATACAGGTACAAATAAAGTTATAGGCATTCAGGCCCCCTGGTGGCGATCTACAATATCTTTGAATGCTAAAAGCTTTTCAAAGATGAAGAATCTTAGATATATTTCTATGAGAGGAACATCCTTTTCTGGAGATATTGATTATCTCTCCAACCAGTTGAGGTGGCTCGATTGGCAAGAATGTCCGTTGCAATGTTTTCCATCCGATTTTCAAGCAAACAAACTTGTGGAGCTCAACATTAGTAGGGGCAAAATCAGACGACTATGGGAGGGACGGAAGAATTTTTCAAGGCTAACATGTATGAATTTAAGTGACTGTGAATCCCTAAAGGAACTCCCAGACTTGGGTGGAATCCCTAACTTGAAAGAGTTGAATTTAAGTGGCTGTGAATCCCTAAAGGAACTCCCAGACTTGAGTGGAATCCCTAACTTGAAAGAGTTGGATTTACGTGGCTGTACATCCCTAGAGGAACTCCCAGACTTGAGTGGAATCCCTAACTTGAAGGGGTTGCTCTTACGTGGCTGTACATCCCTAAAGAAACTCCCATACTTGAGTGGAATCCCTAACTTGAAAGGGTTGAATTTAAGCGGTTGTGTATCCCTAAAAGAACTCCCAGACTTTTGTGGAATCCCTAACTTGAAAGAGTTGGAACTATTTGAATGTATCGGTTTAGTTGAGGTTCCTGATTCAATTCGATTCCTTCATAAGCTTGTTACCTTGAATGTGGGCGGTTGCTTTAACCTTATTATGTTTCCCAGAAAAATCAACTTGAAATCTATAGAAACAATCTCTATTAGGTATTGCAAGCTTGAAGAATTTTCAGAAGTTGGGGAAGAGATGGGTTTCTTGAGAAAATTGGATCTAAGAGGCACTTGCATTAAAGAATTGCATCCGTCCATTACAAGGCTTATCAGGCTGGAAAAGTTGGACCTAACGGACAGTCAAAATCTTATAACCCTGCCATATAATATTTATGAGTTGCACAATCTAAAGGCTCTTTATGCGGGAGGATGCTCAAAACTAGCTACGTTTCCAAAAATTCTAGTAAAGATGGATTCTTTGAGAGAACTCTCTCTTCAGGGCAGTGACATAAGAGAATTGGATGAGTCAATTGAAAATCTCATTGGGCTTGAATATTTGGATCTTACTGAATGCAAAAATCTCACAACTCTACCGTGCAGCATTTATGGGTTGCAAAATTTAGGGTTTCTTGGTCTTGGTGAATGCTCAAAACTTGTCAGATTTCCAACAAATACAAAAAATTTGAATGACGATGGTTGCTCACTATCACTTCCCAAGCTACGTGTGTTCAGTATCGCAGGGTGCAGTTTATTATCAGATTGTGATTTCCTGATGACTCTTGATTGCTGGGAAACATTATGGTTGCTTGATCTGTCAAGAAACAATTTTGTTAGTCTTCCTGCTTGCCTCACCAAATTTGTCAACTTGCACGGGCTTGACTTGCGGGATTGCAAGAGACTCCGAGAAATTCCGGAGCTTCCACCAAACGTTGATGACCTAAGGATAAGTGGTTGTGAATTACTGGAGGAGAGATTCTCGACATTGACAAATCGTGGAGCTGGGACAAATAGTCGCCGTCAATTCAAGAGCCAACTCCAAAACGACACCGCACCGCGGAAGAACCATCGCTGGCTGGGTTGTTTCGGCTTTCTCTCTCTATGGAACCAACACCAACGAATTCTCCGAAACGACAACGCCTTGCCGCCGCGGGGGGCAAGCATCGATGGTCCCCATGTTCCTCTGCTTCAACACCAAGCAGAGGAACCAACTGACCTCTGA

Protein Analysis

1146

Amino Acids

129.95

Weight (kDa)

6.3

Isoelectric Point (pI)

43.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 37 - 198 7.7e-54 TIR domain
TIR_2 PF13676 39 - 131 9.3e-15 TIR domain
NB-ARC PF00931 223 - 385 2.1e-21 NB-ARC domain
WHD_ROQ1 PF23282 455 - 525 1.9e-16 Disease resistance protein Roq1-like, winged-helix domain
LRR_4 PF12799 666 - 709 4e-07 Leucine Rich repeats (2 copies)
LRR_13 PF23286 804 - 888 3.3e-07 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 830 - 906 1.5e-08 Leucine-rich repeat region
LRR_13 PF23286 885 - 1046 5.6e-18 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 901 - 969 2.4e-06 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000549)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G04820 AT1G50010 AT4G14960 AT4G14960
fragaria_vesca FvH4_1g18660 FvH4_2g03140 FvH4_5g33560 FvH4_6g48940 FvH4_6g48940
malus_domestica MD02G1204800.v1.1 MD05G1106800.v1.1 MD08G1210600.v1.1 MD09G1049500.v1.1 MD10G1111600.v1.1 MD15G1398700.v1.1 MD17G1049700.v1.1
prunus_persica Prupe.1G542600_v2.0.a1 Prupe.3G269300_v2.0.a1 Prupe.6G212700_v2.0.a1 Prupe.8G150900_v2.0.a1
pyrus_communis pycom05g10370 pycom08g18150 pycom10g09640 pycom10g09660 pycom111g04050 pycom15g35470 pycom17g04460
rosa_chinensis RchiOBHm_Chr2g0109481 RchiOBHm_Chr2g0168751 RchiOBHm_Chr3g0453071 RchiOBHm_Chr3g0460741 RchiOBHm_Chr3g0467971 RchiOBHm_Chr4g0400901 RchiOBHm_Chr6g0248011 RchiOBHm_Chr7g0232381 RchiOBHm_Chr7g0238121
rosa_laevigata RLG00000001350 RLG00000009167 RLG00000015163 RLG00000017708 RLG00000017741 RLG00000021807 RLG00000023849 RLG00000026518
rosa_multiflora Rmu_sc0000332.1_g000021 Rmu_sc0000332.1_g000029 Rmu_sc0002416.1_g000007 Rmu_sc0002611.1_g000001 Rmu_sc0003113.1_g000016 Rmu_sc0004780.1_g000001 Rmu_sc0004805.1_g000035 Rmu_sc0010633.1_g000010 Rmu_sc0011069.1_g000003 Rmu_sc0016170.1_g000002 Rmu_sc0025757.1_g000003 Rmu_ssc0000484.1_g000003
rosa_roxburghii Rroxscaffold_2G00134200 Rroxscaffold_4G00321030 Rroxscaffold_7G00213500
rosa_rugosa Rorug01G0283100 Rorug02G0160500 Rorug02G0535300 Rorug04G0027000 Rorug04G0328100 Rorug05G0388700 Rorug05G0530200 Rorug06G0489200 Rorug07G0104900 Rorug07G0209400 Rorug07G0274500
rosa_samantha Rh2BG222800 Rh2BG618900 Rh2CG214400 Rh2CG587200 Rh6BG040200 Rh6CG206500 Rh6DG035600 Rh7BG401600 Rh7BG401700 Rh7DG418400
rosa_wichuraiana Rw2G050240 Rw6G003920 Rw7G035430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 917, 2594
AasI GACNNNNNNGTC 1 cut(s) 905
AccI GTMKAC 5 cut(s) 170, 655, 666, 1410, 1623
AccII CGCG 2 cut(s) 3277, 3370
AccIII TCCGGA 1 cut(s) 3133
AclI AACGTT 2 cut(s) 1054, 3150
AclWI GGATC 2 cut(s) 2508, 2790
AcuI CTGAAG 2 cut(s) 187, 2702
AfaI GTAC 5 cut(s) 411, 548, 1651, 2098, 2167
AfiI CCNNNNNNNGG 8 cut(s) 315, 1683, 1984, 1985, 2544, 3276, 3371, 3421
AflIII ACRYGT 3 cut(s) 1189, 1937, 2944
AhdI GACNNNNNGTC 1 cut(s) 3231
AjiI CACGTC 1 cut(s) 964
AjnI CCWGG 1 cut(s) 1679
AjuI GAANNNNNNNTTGG 2 cut(s) 2748, 2780
Alw21I GWGCWC 2 cut(s) 249, 1875
Alw26I GTCTC 5 cut(s) 191, 545, 819, 841, 3112
AlwI GGATC 2 cut(s) 2508, 2790
Ama87I CYCGRG 3 cut(s) 591, 1018, 1586
Aor13HI TCCGGA 1 cut(s) 3133
AoxI GGCC 5 cut(s) 541, 826, 1015, 1210, 1674
ApeKI GCWGC 2 cut(s) 1596, 2820
Asp700I GAANNNNTTC 1 cut(s) 1371
AspS9I GGNCC 6 cut(s) 541, 1016, 1301, 1675, 2569, 3389
AsuC2I CCSGG 4 cut(s) 1019, 1020, 1587, 1588
AsuHPI GGTGA 6 cut(s) 83, 446, 898, 1566, 2873, 3064
AvaI CYCGRG 3 cut(s) 591, 1018, 1586
AvaII GGWCC 3 cut(s) 1301, 2569, 3389
AxyI CCTNAGG 2 cut(s) 1305, 3161
BanII GRGCYC 2 cut(s) 249, 1875
BbsI GAAGAC 4 cut(s) 109, 237, 280, 3050
Bbv12I GWGCWC 2 cut(s) 249, 1875
BbvI GCAGC 2 cut(s) 1608, 2832
BccI CCATC 8 cut(s) 519, 1006, 1849, 2473, 2686, 2909, 3294, 3380
BceAI ACGGC 1 cut(s) 3222
BciT130I CCWGG 1 cut(s) 1681
BciVI GTATCC 2 cut(s) 133, 2248
BclI TGATCA 3 cut(s) 88, 454, 1513
BcnI CCSGG 4 cut(s) 1019, 1020, 1587, 1588
BcoDI GTCTC 5 cut(s) 191, 545, 819, 841, 3112
BfaI CTAG 7 cut(s) 830, 975, 1349, 1430, 2105, 2663, 2684
BfmI CTRYAG 2 cut(s) 349, 2418
BfuI GTATCC 2 cut(s) 133, 2248
BisI GCNGC 4 cut(s) 1597, 2821, 3365, 3368
BlpI GCTNAGC 1 cut(s) 218
BlsI GCNGC 4 cut(s) 1598, 2822, 3366, 3369
Bme1390I CCNGG 5 cut(s) 1019, 1020, 1587, 1588, 1681
Bme18I GGWCC 3 cut(s) 1301, 2569, 3389
BmeRI GACNNNNNGTC 1 cut(s) 3231
BmeT110I CYCGRG 3 cut(s) 591, 1018, 1586
BmgBI CACGTC 1 cut(s) 964
BmgT120I GGNCC 6 cut(s) 541, 1016, 1301, 1675, 2569, 3389
BmiI GGNNCC 5 cut(s) 1677, 2322, 3326, 3391, 3426
BmrFI CCNGG 5 cut(s) 1019, 1020, 1587, 1588, 1681
BmsI GCATC 7 cut(s) 53, 486, 1333, 1515, 2539, 2642, 3390
BoxI GACNNNNGTC 1 cut(s) 966
BpiI GAAGAC 4 cut(s) 109, 237, 280, 3050
BplI GAGNNNNNCTC 4 cut(s) 481, 513, 3182, 3214
BpmI CTGGAG 2 cut(s) 1791, 3206
Bpu1102I GCTNAGC 1 cut(s) 218
BpuEI CTTGAG 6 cut(s) 295, 521, 2074, 2143, 2212, 2509
BpuMI CCSGG 4 cut(s) 1019, 1020, 1587, 1588
Bsa29I ATCGAT 1 cut(s) 3384
BsaAI YACGTR 3 cut(s) 2090, 2159, 2945
BsaI GGTCTC 2 cut(s) 191, 819
BsaJI CCNNGG 8 cut(s) 684, 901, 1018, 1207, 1586, 1679, 3275, 3368
BsaWI WCCGGW 1 cut(s) 3133
BsaXI ACNNNNNCTCC 6 cut(s) 231, 261, 530, 560, 3327, 3357
Bsc4I CCNNNNNNNGG 8 cut(s) 315, 1683, 1984, 1985, 2544, 3276, 3371, 3421
Bse118I RCCGGY 1 cut(s) 543
Bse1I ACTGG 5 cut(s) 51, 336, 727, 1795, 3189
Bse21I CCTNAGG 2 cut(s) 1305, 3161
Bse3DI GCAATG 1 cut(s) 1838
BseAI TCCGGA 1 cut(s) 3133
BseBI CCWGG 1 cut(s) 1681
BseCI ATCGAT 1 cut(s) 3384
BseDI CCNNGG 8 cut(s) 684, 901, 1018, 1207, 1586, 1679, 3275, 3368
BseLI CCNNNNNNNGG 8 cut(s) 315, 1683, 1984, 1985, 2544, 3276, 3371, 3421
BseMI GCAATG 1 cut(s) 1838
BseMII CTCAG 3 cut(s) 113, 417, 1319
BseNI ACTGG 5 cut(s) 51, 336, 727, 1795, 3189
BseRI GAGGAG 6 cut(s) 239, 240, 278, 551, 1280, 3203
BseXI GCAGC 2 cut(s) 1608, 2832
BseYI CCCAGC 3 cut(s) 3009, 3221, 3295
BsgI GTGCAG 2 cut(s) 2839, 2986
Bsh1236I CGCG 2 cut(s) 3277, 3370
Bsh1285I CGRYCG 1 cut(s) 1447
BshFI GGCC 5 cut(s) 543, 828, 1017, 1212, 1676
BshVI ATCGAT 1 cut(s) 3384
BsiEI CGRYCG 1 cut(s) 1447
BsiHKAI GWGCWC 2 cut(s) 249, 1875
BsiHKCI CYCGRG 3 cut(s) 591, 1018, 1586
BsiSI CCGG 4 cut(s) 544, 1019, 1587, 3134
BslFI GGGAC 5 cut(s) 733, 884, 1926, 3238, 3375
BslI CCNNNNNNNGG 8 cut(s) 315, 1683, 1984, 1985, 2544, 3276, 3371, 3421
BsmAI GTCTC 5 cut(s) 191, 545, 819, 841, 3112
BsmFI GGGAC 5 cut(s) 733, 884, 1926, 3238, 3375
BsmI GAATGC 6 cut(s) 933, 1142, 1668, 1711, 2798, 2870
BsnI GGCC 5 cut(s) 543, 828, 1017, 1212, 1676
Bso31I GGTCTC 2 cut(s) 191, 819
BsoBI CYCGRG 3 cut(s) 591, 1018, 1586
Bsp1286I GDGCHC 2 cut(s) 249, 1875
Bsp13I TCCGGA 1 cut(s) 3133
Bsp1407I TGTACA 2 cut(s) 2096, 2165
Bsp143I GATC 9 cut(s) 88, 160, 454, 1108, 1513, 1688, 2500, 2782, 3031
Bsp1720I GCTNAGC 1 cut(s) 218
BspANI GGCC 5 cut(s) 543, 828, 1017, 1212, 1676
BspCNI CTCAG 3 cut(s) 114, 416, 1318
BspDI ATCGAT 1 cut(s) 3384
BspEI TCCGGA 1 cut(s) 3133
BspFNI CGCG 2 cut(s) 3277, 3370
BspHI TCATGA 1 cut(s) 1069
BspLI GGNNCC 5 cut(s) 1677, 2322, 3326, 3391, 3426
BspMAI CTGCAG 1 cut(s) 353
BspPI GGATC 2 cut(s) 2508, 2790
BspTNI GGTCTC 2 cut(s) 191, 819
BsrDI GCAATG 1 cut(s) 1838
BsrFI RCCGGY 1 cut(s) 543
BsrGI TGTACA 2 cut(s) 2096, 2165
BsrI ACTGG 5 cut(s) 51, 336, 727, 1795, 3189
BssAI RCCGGY 1 cut(s) 543
BssECI CCNNGG 8 cut(s) 684, 901, 1018, 1207, 1586, 1679, 3275, 3368
BssMI GATC 9 cut(s) 88, 160, 454, 1108, 1513, 1688, 2500, 2782, 3031
BssNAI GTATAC 2 cut(s) 171, 656
BssT1I CCWWGG 2 cut(s) 901, 1207
Bst1107I GTATAC 2 cut(s) 171, 656
Bst2UI CCWGG 1 cut(s) 1681
Bst4CI ACNGT 5 cut(s) 671, 1415, 1958, 2582, 2817
Bst6I CTCTTC 2 cut(s) 2469, 2721
BstAUI TGTACA 2 cut(s) 2096, 2165
BstBAI YACGTR 3 cut(s) 2090, 2159, 2945
BstC8I GCNNGC 9 cut(s) 14, 1136, 1140, 1387, 1438, 2447, 3067, 3293, 3379
BstDSI CCRYGG 2 cut(s) 3275, 3368
BstFNI CGCG 2 cut(s) 3277, 3370
BstKTI GATC 9 cut(s) 91, 163, 457, 1111, 1516, 1691, 2503, 2785, 3034
BstMAI GTCTC 5 cut(s) 191, 545, 819, 841, 3112
BstMBI GATC 9 cut(s) 88, 160, 454, 1108, 1513, 1688, 2500, 2782, 3031
BstMCI CGRYCG 1 cut(s) 1447
BstMWI GCNNNNNNNGC 7 cut(s) 13, 708, 1446, 1813, 2554, 2642, 3103
BstNI CCWGG 1 cut(s) 1681
BstNSI RCATGY 3 cut(s) 1142, 1193, 1941
BstPAI GACNNNNGTC 1 cut(s) 966
BstSCI CCNGG 5 cut(s) 1017, 1018, 1585, 1586, 1679
BstSFI CTRYAG 2 cut(s) 349, 2418
BstUI CGCG 2 cut(s) 3277, 3370
BstV1I GCAGC 2 cut(s) 1608, 2832
BstV2I GAAGAC 4 cut(s) 109, 237, 280, 3050
BstX2I RGATCY 2 cut(s) 2500, 2782
BstYI RGATCY 2 cut(s) 2500, 2782
BstZ17I GTATAC 2 cut(s) 171, 656
Bsu15I ATCGAT 1 cut(s) 3384
Bsu36I CCTNAGG 2 cut(s) 1305, 3161
BsuI GTATCC 2 cut(s) 133, 2248
BsuRI GGCC 5 cut(s) 543, 828, 1017, 1212, 1676
BsuTUI ATCGAT 1 cut(s) 3384
BtgI CCRYGG 2 cut(s) 3275, 3368
BtgZI GCGATG 1 cut(s) 3272
BtrI CACGTC 1 cut(s) 964
BtsI GCAGTG 2 cut(s) 898, 2731
BtsIMutI CAGTG 2 cut(s) 898, 2731
Cac8I GCNNGC 9 cut(s) 14, 1136, 1140, 1387, 1438, 2447, 3067, 3293, 3379
CciI TCATGA 1 cut(s) 1069
Cfr10I RCCGGY 1 cut(s) 543
Cfr13I GGNCC 6 cut(s) 541, 1016, 1301, 1675, 2569, 3389
Cfr42I CCGCGG 2 cut(s) 3278, 3371
Cfr9I CCCGGG 2 cut(s) 1018, 1586
ClaI ATCGAT 1 cut(s) 3384
CseI GACGC 1 cut(s) 446
Csp6I GTAC 5 cut(s) 410, 547, 1650, 2097, 2166
CviQI GTAC 5 cut(s) 410, 547, 1650, 2097, 2166
DpnI GATC 9 cut(s) 90, 162, 456, 1110, 1515, 1690, 2502, 2784, 3033
DpnII GATC 9 cut(s) 88, 160, 454, 1108, 1513, 1688, 2500, 2782, 3031
DraI TTTAAA 3 cut(s) 678, 1362, 1396
DrdI GACNNNNNNGTC 1 cut(s) 905
DriI GACNNNNNGTC 1 cut(s) 3231
DseDI GACNNNNNNGTC 1 cut(s) 905
Eam1104I CTCTTC 2 cut(s) 2469, 2721
Eam1105I GACNNNNNGTC 1 cut(s) 3231
EarI CTCTTC 2 cut(s) 2469, 2721
Ecl136II GAGCTC 2 cut(s) 247, 1873
Eco130I CCWWGG 2 cut(s) 901, 1207
Eco147I AGGCCT 1 cut(s) 1212
Eco24I GRGCYC 2 cut(s) 249, 1875
Eco31I GGTCTC 2 cut(s) 191, 819
Eco32I GATATC 1 cut(s) 1381
Eco47I GGWCC 3 cut(s) 1301, 2569, 3389
Eco53kI GAGCTC 2 cut(s) 247, 1873
Eco57I CTGAAG 2 cut(s) 187, 2702
Eco81I CCTNAGG 2 cut(s) 1305, 3161
Eco88I CYCGRG 3 cut(s) 591, 1018, 1586
EcoICRI GAGCTC 2 cut(s) 247, 1873
EcoO109I RGGNCCY 2 cut(s) 1301, 1675
EcoRI GAATTC 2 cut(s) 1029, 3339
EcoRII CCWGG 1 cut(s) 1679
EcoRV GATATC 1 cut(s) 1381
EcoT14I CCWWGG 2 cut(s) 901, 1207
EcoT22I ATGCAT 2 cut(s) 1144, 1530
EcoT38I GRGCYC 2 cut(s) 249, 1875
ErhI CCWWGG 2 cut(s) 901, 1207
FalI AAGNNNNNCTT 6 cut(s) 834, 866, 1389, 1421, 2497, 2529
FaqI GGGAC 5 cut(s) 733, 884, 1926, 3238, 3375
FauI CCCGC 3 cut(s) 2638, 3099, 3363
FauNDI CATATG 1 cut(s) 713
FbaI TGATCA 3 cut(s) 88, 454, 1513
FblI GTMKAC 5 cut(s) 170, 655, 666, 1410, 1623
Fnu4HI GCNGC 4 cut(s) 1597, 2821, 3365, 3368
FriOI GRGCYC 2 cut(s) 249, 1875
Fsp4HI GCNGC 4 cut(s) 1597, 2821, 3365, 3368
FspBI CTAG 7 cut(s) 830, 975, 1349, 1430, 2105, 2663, 2684
GluI GCNGC 4 cut(s) 1597, 2821, 3365, 3368
GsaI CCCAGC 3 cut(s) 3013, 3225, 3299
GsuI CTGGAG 2 cut(s) 1791, 3206
HaeIII GGCC 5 cut(s) 543, 828, 1017, 1212, 1676
HapII CCGG 4 cut(s) 544, 1019, 1587, 3134
HgaI GACGC 1 cut(s) 446
HincII GTYRAC 3 cut(s) 667, 898, 3085
HindII GTYRAC 3 cut(s) 667, 898, 3085
HindIII AAGCTT 6 cut(s) 215, 1403, 1434, 1714, 2348, 2447
HpaII CCGG 4 cut(s) 544, 1019, 1587, 3134
HphI GGTGA 6 cut(s) 83, 446, 898, 1566, 2873, 3064
Hpy166II GTNNAC 8 cut(s) 171, 601, 656, 667, 898, 1411, 1624, 3085
Hpy8I GTNNAC 8 cut(s) 171, 601, 656, 667, 898, 1411, 1624, 3085
Hpy99I CGWCG 1 cut(s) 287
HpyAV CCTTC 7 cut(s) 211, 487, 615, 877, 1223, 2137, 2351
HpyCH4III ACNGT 5 cut(s) 671, 1415, 1958, 2582, 2817
HpyCH4IV ACGT 8 cut(s) 621, 963, 1054, 2089, 2158, 2669, 2944, 3150
HpyF10VI GCNNNNNNNGC 7 cut(s) 13, 708, 1446, 1813, 2554, 2642, 3103
HpySE526I ACGT 8 cut(s) 621, 963, 1054, 2089, 2158, 2669, 2944, 3150
Kpn2I TCCGGA 1 cut(s) 3133
Ksp22I TGATCA 3 cut(s) 88, 454, 1513
KspI CCGCGG 2 cut(s) 3278, 3371
Kzo9I GATC 9 cut(s) 88, 160, 454, 1108, 1513, 1688, 2500, 2782, 3031
LmnI GCTCC 6 cut(s) 4, 252, 403, 1870, 3136, 3218
Lsp1109I GCAGC 2 cut(s) 1608, 2832
LweI GCATC 7 cut(s) 53, 486, 1333, 1515, 2539, 2642, 3390
MaeI CTAG 7 cut(s) 830, 975, 1349, 1430, 2105, 2663, 2684
MaeII ACGT 8 cut(s) 621, 963, 1054, 2089, 2158, 2669, 2944, 3150
MaeIII GTNAC 5 cut(s) 434, 1572, 1952, 2353, 2726
MalI GATC 9 cut(s) 90, 162, 456, 1110, 1515, 1690, 2502, 2784, 3033
MboI GATC 9 cut(s) 88, 160, 454, 1108, 1513, 1688, 2500, 2782, 3031
MfeI CAATTG 5 cut(s) 258, 636, 744, 1475, 2751
MflI RGATCY 2 cut(s) 2500, 2782
MhlI GDGCHC 2 cut(s) 249, 1875
MlyI GAGTC 9 cut(s) 275, 656, 866, 915, 976, 1580, 2756, 2992, 3114
MmeI TCCRAC 5 cut(s) 1815, 2061, 2268, 2547, 2912
Mph1103I ATGCAT 2 cut(s) 1144, 1530
MroI TCCGGA 1 cut(s) 3133
MroXI GAANNNNTTC 1 cut(s) 1371
MslI CAYNNNNRTG 2 cut(s) 613, 822
MspA1I CMGCKG 2 cut(s) 3277, 3370
MspI CCGG 4 cut(s) 544, 1019, 1587, 3134
MspR9I CCNGG 5 cut(s) 1019, 1020, 1587, 1588, 1681
MunI CAATTG 5 cut(s) 258, 636, 744, 1475, 2751
Mva1269I GAATGC 6 cut(s) 933, 1142, 1668, 1711, 2798, 2870
MvaI CCWGG 1 cut(s) 1681
MvnI CGCG 2 cut(s) 3277, 3370
MwoI GCNNNNNNNGC 7 cut(s) 13, 708, 1446, 1813, 2554, 2642, 3103
NciI CCSGG 4 cut(s) 1019, 1020, 1587, 1588
NdeI CATATG 1 cut(s) 713
NdeII GATC 9 cut(s) 88, 160, 454, 1108, 1513, 1688, 2500, 2782, 3031
NlaIV GGNNCC 5 cut(s) 1677, 2322, 3326, 3391, 3426
NmuCI GTSAC 4 cut(s) 434, 1572, 1952, 2726
NsiI ATGCAT 2 cut(s) 1144, 1530
NspI RCATGY 3 cut(s) 1142, 1193, 1941
PaeI GCATGC 1 cut(s) 1142
PagI TCATGA 1 cut(s) 1069
PceI AGGCCT 1 cut(s) 1212
PciI ACATGT 2 cut(s) 1189, 1937
PcsI WCGNNNNNNNCGW 1 cut(s) 3343
PctI GAATGC 6 cut(s) 933, 1142, 1668, 1711, 2798, 2870
PdmI GAANNNNTTC 1 cut(s) 1371
PkrI GCNGC 4 cut(s) 1598, 2822, 3366, 3369
PleI GAGTC 9 cut(s) 274, 655, 866, 914, 975, 1579, 2755, 2992, 3114
PpsI GAGTC 9 cut(s) 274, 655, 866, 914, 975, 1579, 2755, 2992, 3114
Ppu21I YACGTR 3 cut(s) 2090, 2159, 2945
PpuMI RGGWCCY 1 cut(s) 1301
PscI ACATGT 2 cut(s) 1189, 1937
PshAI GACNNNNGTC 1 cut(s) 966
PsiI TTATAA 2 cut(s) 917, 2594
Psp124BI GAGCTC 2 cut(s) 249, 1875
Psp1406I AACGTT 2 cut(s) 1054, 3150
Psp5II RGGWCCY 1 cut(s) 1301
Psp6I CCWGG 1 cut(s) 1679
PspFI CCCAGC 3 cut(s) 3009, 3221, 3295
PspGI CCWGG 1 cut(s) 1679
PspN4I GGNNCC 5 cut(s) 1677, 2322, 3326, 3391, 3426
PspPI GGNCC 6 cut(s) 541, 1016, 1301, 1675, 2569, 3389
PspPPI RGGWCCY 1 cut(s) 1301
PstI CTGCAG 1 cut(s) 353
PsuI RGATCY 2 cut(s) 2500, 2782
RsaI GTAC 5 cut(s) 411, 548, 1651, 2098, 2167
RsaNI GTAC 5 cut(s) 410, 547, 1650, 2097, 2166
RseI CAYNNNNRTG 2 cut(s) 613, 822
SacI GAGCTC 2 cut(s) 249, 1875
SacII CCGCGG 2 cut(s) 3278, 3371
SalI GTCGAC 1 cut(s) 665
SatI GCNGC 4 cut(s) 1597, 2821, 3365, 3368
Sau3AI GATC 9 cut(s) 88, 160, 454, 1108, 1513, 1688, 2500, 2782, 3031
Sau96I GGNCC 6 cut(s) 541, 1016, 1301, 1675, 2569, 3389
SchI GAGTC 9 cut(s) 275, 656, 866, 915, 976, 1580, 2756, 2992, 3114
ScrFI CCNGG 5 cut(s) 1019, 1020, 1587, 1588, 1681
SduI GDGCHC 2 cut(s) 249, 1875
SfaNI GCATC 7 cut(s) 53, 486, 1333, 1515, 2539, 2642, 3390
SfcI CTRYAG 2 cut(s) 349, 2418
Sfr303I CCGCGG 2 cut(s) 3278, 3371
SgrBI CCGCGG 2 cut(s) 3278, 3371
SinI GGWCC 3 cut(s) 1301, 2569, 3389
SmaI CCCGGG 2 cut(s) 1020, 1588
SmiMI CAYNNNNRTG 2 cut(s) 613, 822
SmlI CTYRAG 6 cut(s) 310, 500, 2053, 2122, 2191, 2488
SmoI CTYRAG 6 cut(s) 310, 500, 2053, 2122, 2191, 2488
SphI GCATGC 1 cut(s) 1142
SrfI GCCCGGGC 1 cut(s) 1020
SseBI AGGCCT 1 cut(s) 1212
SspI AATATT 3 cut(s) 694, 2611, 2777
SspMI CTAG 7 cut(s) 830, 975, 1349, 1430, 2105, 2663, 2684
SstI GAGCTC 2 cut(s) 249, 1875
StuI AGGCCT 1 cut(s) 1212
StyD4I CCNGG 5 cut(s) 1017, 1018, 1585, 1586, 1679
StyI CCWWGG 2 cut(s) 901, 1207
TaaI ACNGT 5 cut(s) 671, 1415, 1958, 2582, 2817
TaiI ACGT 8 cut(s) 624, 966, 1057, 2092, 2161, 2672, 2947, 3153
TaqI TCGA 7 cut(s) 130, 534, 666, 1809, 2335, 3200, 3384
TatI WGTACW 2 cut(s) 2096, 2165
TauI GCSGC 2 cut(s) 3367, 3370
TscAI CASTG 2 cut(s) 898, 2731
TseFI GTSAC 4 cut(s) 434, 1572, 1952, 2726
TseI GCWGC 2 cut(s) 1596, 2820
Tsp45I GTSAC 4 cut(s) 434, 1572, 1952, 2726
TspGWI ACGGA 5 cut(s) 991, 1815, 1930, 2523, 2591
TspMI CCCGGG 2 cut(s) 1018, 1586
TspRI CASTG 2 cut(s) 898, 2731
VpaK11BI GGWCC 3 cut(s) 1301, 2569, 3389
XceI RCATGY 3 cut(s) 1142, 1193, 1941
XmaI CCCGGG 2 cut(s) 1018, 1586
XmiI GTMKAC 5 cut(s) 170, 655, 666, 1410, 1623
XmnI GAANNNNTTC 1 cut(s) 1371
XspI CTAG 7 cut(s) 830, 975, 1349, 1430, 2105, 2663, 2684
Zsp2I ATGCAT 2 cut(s) 1144, 1530
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.