Rorug04G0328100

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0000549
Physical Location & Seq
Forward (+)
50291805 .. 50292812
1008 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0328100.1

Sequence Viewer

Length: 1008 bp
ATGGAGGGCACGGAGGACTGGATGGTTGCAGATGTTATTGATTCTGAAAGTAAGGAGTGGGTTCTTGATGTGTTGAGTGAACTCTTTACGGAGGCAGAGGTCAGTCGTATTGCAGCAATTCCTTTGAGTTTGCGTCAGGCTGAAGATAGATTGGTATGGCATTTTGATGCCAAAGGAGTCTTTAACGTGAAAAGTGGTTATCACGTCTACCGCAACTCATTGAAAATGTATCAAAGAGCTTCAACGTCATCTACGCTTAGTGGGGGTCCGTTGCGTCAGTATTGGAGTAAGGTCTGGAAGTCGGTGGTGCCACCTAGAGTCAAGGTGTTTGTTTGGCGTCTTCTTCTGGATGCTCTGCCCACAAGATCAGCCCTTCTAATACACAAGGTTAGTTTGCCTAGTTATGATTGTGTTTTATGTGGTAATGGGATGGAAACAGGCAAACATCTATTTAAAGATTGTGGGGCCCTCCAATGTTTTTGGAACTTTTGTCCCCTGAAGCTCAAGTCAATGGTTCATGCAGCCCATGATTTACGTACCTGGGTGCTTGATATGTTTGATAGACTGAGTGGTGATCAAGTTGACTATTTTTGCATGGCACTGTGGTTGATATGGAATGAACGTAATAATGTAATTTGGAAAGGTGGGCACTTTCAGCCTATGCATCTGATAGAGTGGTGCCGGCAGAAGTTGGAGGTTTTTCAGTGTTACCATCCAAAGGCAACTAGGAAAAAGAAGAGGTCATTGACCAAGTGGGAATGCCCACCGCGAGGACGACTGAAAATTAACATTGATGGCGCTTATAGGGCTGATTGTGGTGTTGGAGGTATTGGGGTGGTCGTCAGAGATGATTTGGGCATGGGTATTGCAGCTATTGCAAGGCCTTTTCAATTCTACATGCACACTCGGCTATTAATATGGAGGCTGAGGCGTGTAGAGCTGGTCTTCTTCTTAGTATACACCAAGGTTGGACGGAAATTGACATTGAGAGCGACTCTGCCCTTCTGA

Protein Analysis

335

Amino Acids

38.86

Weight (kDa)

9.48

Isoelectric Point (pI)

38.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RVT PF13966 91 - 161 2.1e-15 zinc-binding in reverse transcriptase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000549)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G04820 AT1G50010 AT4G14960 AT4G14960
fragaria_vesca FvH4_1g18660 FvH4_2g03140 FvH4_5g33560 FvH4_6g48940 FvH4_6g48940
malus_domestica MD02G1204800.v1.1 MD05G1106800.v1.1 MD08G1210600.v1.1 MD09G1049500.v1.1 MD10G1111600.v1.1 MD15G1398700.v1.1 MD17G1049700.v1.1
prunus_persica Prupe.1G542600_v2.0.a1 Prupe.3G269300_v2.0.a1 Prupe.6G212700_v2.0.a1 Prupe.8G150900_v2.0.a1
pyrus_communis pycom05g10370 pycom08g18150 pycom10g09640 pycom10g09660 pycom111g04050 pycom15g35470 pycom17g04460
rosa_chinensis RchiOBHm_Chr2g0109481 RchiOBHm_Chr2g0168751 RchiOBHm_Chr3g0453071 RchiOBHm_Chr3g0460741 RchiOBHm_Chr3g0467971 RchiOBHm_Chr4g0400901 RchiOBHm_Chr6g0248011 RchiOBHm_Chr7g0232381 RchiOBHm_Chr7g0238121
rosa_laevigata RLG00000001350 RLG00000009167 RLG00000015163 RLG00000017708 RLG00000017741 RLG00000021807 RLG00000023849 RLG00000026518
rosa_multiflora Rmu_sc0000332.1_g000021 Rmu_sc0000332.1_g000029 Rmu_sc0002416.1_g000007 Rmu_sc0002611.1_g000001 Rmu_sc0003113.1_g000016 Rmu_sc0004780.1_g000001 Rmu_sc0004805.1_g000035 Rmu_sc0010633.1_g000010 Rmu_sc0011069.1_g000003 Rmu_sc0016170.1_g000002 Rmu_sc0025757.1_g000003 Rmu_ssc0000484.1_g000003
rosa_roxburghii Rroxscaffold_2G00134200 Rroxscaffold_4G00321030 Rroxscaffold_7G00213500
rosa_rugosa Rorug01G0283100 Rorug02G0160500 Rorug02G0535300 Rorug04G0027000 Rorug04G0328100 Rorug05G0388700 Rorug05G0530200 Rorug06G0489200 Rorug07G0104900 Rorug07G0209400 Rorug07G0274500
rosa_samantha Rh2BG222800 Rh2BG618900 Rh2CG214400 Rh2CG587200 Rh6BG040200 Rh6CG206500 Rh6DG035600 Rh7BG401600 Rh7BG401700 Rh7DG418400
rosa_wichuraiana Rw2G050240 Rw6G003920 Rw7G035430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 307, 678
AccI GTMKAC 2 cut(s) 207, 957
AccII CGCG 1 cut(s) 769
AciI CCGC 2 cut(s) 211, 767
AcuI CTGAAG 2 cut(s) 162, 518
AcyI GRCGYC 1 cut(s) 337
AfaI GTAC 1 cut(s) 538
AfiI CCNNNNNNNGG 2 cut(s) 718, 770
AgsI TTSAA 3 cut(s) 223, 243, 890
AjiI CACGTC 1 cut(s) 205
AjnI CCWGG 1 cut(s) 539
AluBI AGCT 4 cut(s) 239, 502, 872, 940
AluI AGCT 4 cut(s) 239, 502, 872, 940
AoxI GGCC 2 cut(s) 465, 881
ApaI GGGCCC 1 cut(s) 469
ApeKI GCWGC 3 cut(s) 113, 521, 869
AseI ATTAAT 1 cut(s) 914
AspLEI GCGC 1 cut(s) 800
AspS9I GGNCC 3 cut(s) 266, 465, 466
AsuHPI GGTGA 1 cut(s) 584
AvaII GGWCC 1 cut(s) 266
BaeGI GKGCMC 3 cut(s) 11, 469, 651
BanI GGYRCC 2 cut(s) 307, 678
BanII GRGCYC 1 cut(s) 469
BbsI GAAGAC 2 cut(s) 332, 937
BbvCI CCTCAGC 1 cut(s) 926
BbvI GCAGC 3 cut(s) 125, 533, 881
BccI CCATC 4 cut(s) 16, 424, 720, 788
BciT130I CCWGG 1 cut(s) 541
BclI TGATCA 1 cut(s) 574
BfaI CTAG 3 cut(s) 315, 399, 726
BfoI RGCGCY 1 cut(s) 801
BisI GCNGC 3 cut(s) 114, 522, 870
BlsI GCNGC 3 cut(s) 115, 523, 871
Bme1390I CCNGG 1 cut(s) 541
Bme18I GGWCC 1 cut(s) 266
BmgBI CACGTC 1 cut(s) 205
BmgT120I GGNCC 3 cut(s) 266, 465, 466
BmiI GGNNCC 5 cut(s) 267, 309, 466, 467, 680
BmrFI CCNGG 1 cut(s) 541
BmsI GCATC 3 cut(s) 157, 340, 673
BpiI GAAGAC 2 cut(s) 332, 937
BplI GAGNNNNNCTC 1 cut(s) 979
Bpu10I CCTNAGC 1 cut(s) 926
BpuEI CTTGAG 1 cut(s) 488
BsaAI YACGTR 1 cut(s) 536
BsaHI GRCGYC 1 cut(s) 337
BsaJI CCNNGG 2 cut(s) 540, 963
Bsc4I CCNNNNNNNGG 2 cut(s) 718, 770
Bse118I RCCGGY 1 cut(s) 681
Bse1I ACTGG 1 cut(s) 23
BseBI CCWGG 1 cut(s) 541
BseDI CCNNGG 2 cut(s) 540, 963
BseGI GGATG 4 cut(s) 27, 355, 435, 712
BseLI CCNNNNNNNGG 2 cut(s) 718, 770
BseMII CTCAG 2 cut(s) 557, 917
BseNI ACTGG 1 cut(s) 23
BseSI GKGCMC 3 cut(s) 11, 469, 651
BseXI GCAGC 3 cut(s) 125, 533, 881
Bsh1236I CGCG 1 cut(s) 769
BshFI GGCC 2 cut(s) 467, 883
BshNI GGYRCC 2 cut(s) 307, 678
BsiSI CCGG 1 cut(s) 682
BslFI GGGAC 1 cut(s) 477
BslI CCNNNNNNNGG 2 cut(s) 718, 770
BsmFI GGGAC 1 cut(s) 477
BsmI GAATGC 1 cut(s) 764
BsnI GGCC 2 cut(s) 467, 883
Bsp120I GGGCCC 1 cut(s) 465
Bsp1286I GDGCHC 3 cut(s) 11, 469, 651
Bsp143I GATC 2 cut(s) 365, 574
BspACI CCGC 2 cut(s) 211, 767
BspANI GGCC 2 cut(s) 467, 883
BspCNI CTCAG 2 cut(s) 558, 918
BspFNI CGCG 1 cut(s) 769
BspLI GGNNCC 5 cut(s) 267, 309, 466, 467, 680
BspT107I GGYRCC 2 cut(s) 307, 678
BsrFI RCCGGY 1 cut(s) 681
BsrI ACTGG 1 cut(s) 23
BssAI RCCGGY 1 cut(s) 681
BssECI CCNNGG 2 cut(s) 540, 963
BssMI GATC 2 cut(s) 365, 574
BssNAI GTATAC 1 cut(s) 958
BssNI GRCGYC 1 cut(s) 337
BssT1I CCWWGG 1 cut(s) 963
Bst1107I GTATAC 1 cut(s) 958
Bst2UI CCWGG 1 cut(s) 541
Bst4CI ACNGT 1 cut(s) 603
Bst6I CTCTTC 1 cut(s) 731
BstACI GRCGYC 1 cut(s) 337
BstAPI GCANNNNNTGC 1 cut(s) 875
BstBAI YACGTR 1 cut(s) 536
BstC8I GCNNGC 1 cut(s) 683
BstDEI CTNAG 4 cut(s) 257, 566, 926, 952
BstF5I GGATG 4 cut(s) 27, 355, 435, 712
BstFNI CGCG 1 cut(s) 769
BstH2I RGCGCY 1 cut(s) 801
BstHHI GCGC 1 cut(s) 800
BstKTI GATC 2 cut(s) 368, 577
BstMBI GATC 2 cut(s) 365, 574
BstMWI GCNNNNNNNGC 5 cut(s) 655, 806, 875, 907, 937
BstNI CCWGG 1 cut(s) 541
BstNSI RCATGY 1 cut(s) 901
BstSCI CCNGG 1 cut(s) 539
BstSLI GKGCMC 3 cut(s) 11, 469, 651
BstSNI TACGTA 1 cut(s) 536
BstUI CGCG 1 cut(s) 769
BstV1I GCAGC 3 cut(s) 125, 533, 881
BstV2I GAAGAC 2 cut(s) 332, 937
BstZ17I GTATAC 1 cut(s) 958
BsuRI GGCC 2 cut(s) 467, 883
BtrI CACGTC 1 cut(s) 205
BtsCI GGATG 4 cut(s) 27, 355, 435, 712
BtsIMutI CAGTG 2 cut(s) 599, 710
Cac8I GCNNGC 1 cut(s) 683
CfoI GCGC 1 cut(s) 800
Cfr10I RCCGGY 1 cut(s) 681
Cfr13I GGNCC 3 cut(s) 266, 465, 466
CseI GACGC 3 cut(s) 122, 263, 326
Csp6I GTAC 1 cut(s) 537
CviAII CATG 5 cut(s) 518, 527, 595, 859, 898
CviQI GTAC 1 cut(s) 537
DdeI CTNAG 4 cut(s) 257, 566, 926, 952
DpnI GATC 2 cut(s) 367, 576
DpnII GATC 2 cut(s) 365, 574
DraI TTTAAA 1 cut(s) 454
Eam1104I CTCTTC 1 cut(s) 731
EarI CTCTTC 1 cut(s) 731
Eco105I TACGTA 1 cut(s) 536
Eco130I CCWWGG 1 cut(s) 963
Eco147I AGGCCT 1 cut(s) 883
Eco24I GRGCYC 1 cut(s) 469
Eco47I GGWCC 1 cut(s) 266
Eco57I CTGAAG 2 cut(s) 162, 518
EcoO109I RGGNCCY 2 cut(s) 465, 466
EcoRII CCWGG 1 cut(s) 539
EcoT14I CCWWGG 1 cut(s) 963
EcoT22I ATGCAT 1 cut(s) 666
EcoT38I GRGCYC 1 cut(s) 469
ErhI CCWWGG 1 cut(s) 963
FaeI CATG 5 cut(s) 521, 530, 598, 862, 901
FaqI GGGAC 1 cut(s) 477
FatI CATG 5 cut(s) 517, 526, 594, 858, 897
FbaI TGATCA 1 cut(s) 574
FblI GTMKAC 2 cut(s) 207, 957
Fnu4HI GCNGC 3 cut(s) 114, 522, 870
FokI GGATG 4 cut(s) 34, 362, 442, 699
FriOI GRGCYC 1 cut(s) 469
Fsp4HI GCNGC 3 cut(s) 114, 522, 870
FspBI CTAG 3 cut(s) 315, 399, 726
GlaI GCGC 1 cut(s) 799
GluI GCNGC 3 cut(s) 114, 522, 870
HaeII RGCGCY 1 cut(s) 801
HaeIII GGCC 2 cut(s) 467, 883
HapII CCGG 1 cut(s) 682
HgaI GACGC 3 cut(s) 122, 263, 326
HhaI GCGC 1 cut(s) 800
Hin1I GRCGYC 1 cut(s) 337
Hin1II CATG 5 cut(s) 521, 530, 598, 862, 901
Hin6I GCGC 1 cut(s) 798
HinP1I GCGC 1 cut(s) 798
HincII GTYRAC 1 cut(s) 583
HindII GTYRAC 1 cut(s) 583
HinfI GANTC 4 cut(s) 41, 177, 318, 994
HpaII CCGG 1 cut(s) 682
HphI GGTGA 1 cut(s) 584
Hpy166II GTNNAC 4 cut(s) 80, 208, 583, 958
Hpy188I TCNGA 4 cut(s) 46, 669, 845, 1007
Hpy188III TCNNGA 3 cut(s) 65, 295, 347
Hpy8I GTNNAC 4 cut(s) 80, 208, 583, 958
HpyAV CCTTC 1 cut(s) 383
HpyCH4III ACNGT 1 cut(s) 603
HpyCH4IV ACGT 5 cut(s) 186, 204, 245, 535, 622
HpyCH4V TGCA 8 cut(s) 29, 113, 521, 594, 664, 869, 878, 901
HpyF10VI GCNNNNNNNGC 5 cut(s) 655, 806, 875, 907, 937
HpyF3I CTNAG 4 cut(s) 257, 566, 926, 952
HpySE526I ACGT 5 cut(s) 186, 204, 245, 535, 622
Hsp92I GRCGYC 1 cut(s) 337
Hsp92II CATG 5 cut(s) 521, 530, 598, 862, 901
HspAI GCGC 1 cut(s) 798
KroI GCCGGC 1 cut(s) 681
KroNI GCCGGC 1 cut(s) 683
Ksp22I TGATCA 1 cut(s) 574
Kzo9I GATC 2 cut(s) 365, 574
Lsp1109I GCAGC 3 cut(s) 125, 533, 881
LweI GCATC 3 cut(s) 157, 340, 673
MaeI CTAG 3 cut(s) 315, 399, 726
MaeII ACGT 5 cut(s) 186, 204, 245, 535, 622
MaeIII GTNAC 1 cut(s) 707
MalI GATC 2 cut(s) 367, 576
MboI GATC 2 cut(s) 365, 574
MboII GAAGA 6 cut(s) 155, 332, 335, 748, 937, 940
MhlI GDGCHC 3 cut(s) 11, 469, 651
MluCI AATT 5 cut(s) 117, 633, 783, 890, 977
MlyI GAGTC 3 cut(s) 186, 327, 988
MmeI TCCRAC 3 cut(s) 672, 802, 949
Mph1103I ATGCAT 1 cut(s) 666
MroNI GCCGGC 1 cut(s) 681
MseI TTAA 4 cut(s) 183, 453, 786, 914
MslI CAYNNNNRTG 1 cut(s) 165
MspI CCGG 1 cut(s) 682
MspR9I CCNGG 1 cut(s) 541
Mva1269I GAATGC 1 cut(s) 764
MvaI CCWGG 1 cut(s) 541
MvnI CGCG 1 cut(s) 769
MwoI GCNNNNNNNGC 5 cut(s) 655, 806, 875, 907, 937
NaeI GCCGGC 1 cut(s) 683
NdeII GATC 2 cut(s) 365, 574
NgoMIV GCCGGC 1 cut(s) 681
NlaIII CATG 5 cut(s) 521, 530, 598, 862, 901
NlaIV GGNNCC 5 cut(s) 267, 309, 466, 467, 680
NmeAIII GCCGAG 1 cut(s) 886
NsiI ATGCAT 1 cut(s) 666
NspI RCATGY 1 cut(s) 901
PceI AGGCCT 1 cut(s) 883
PctI GAATGC 1 cut(s) 764
PdiI GCCGGC 1 cut(s) 683
PfeI GAWTC 1 cut(s) 41
PkrI GCNGC 3 cut(s) 115, 523, 871
PleI GAGTC 3 cut(s) 185, 326, 988
PpsI GAGTC 3 cut(s) 185, 326, 988
Ppu21I YACGTR 1 cut(s) 536
PshBI ATTAAT 1 cut(s) 914
Psp6I CCWGG 1 cut(s) 539
PspGI CCWGG 1 cut(s) 539
PspN4I GGNNCC 5 cut(s) 267, 309, 466, 467, 680
PspOMI GGGCCC 1 cut(s) 465
PspPI GGNCC 3 cut(s) 266, 465, 466
RsaI GTAC 1 cut(s) 538
RsaNI GTAC 1 cut(s) 537
RseI CAYNNNNRTG 1 cut(s) 165
SaqAI TTAA 4 cut(s) 183, 453, 786, 914
SatI GCNGC 3 cut(s) 114, 522, 870
Sau3AI GATC 2 cut(s) 365, 574
Sau96I GGNCC 3 cut(s) 266, 465, 466
SchI GAGTC 3 cut(s) 186, 327, 988
ScrFI CCNGG 1 cut(s) 541
SduI GDGCHC 3 cut(s) 11, 469, 651
SfaNI GCATC 3 cut(s) 157, 340, 673
SinI GGWCC 1 cut(s) 266
SmiMI CAYNNNNRTG 1 cut(s) 165
SmlI CTYRAG 1 cut(s) 503
SmoI CTYRAG 1 cut(s) 503
SnaBI TACGTA 1 cut(s) 536
Sse9I AATT 5 cut(s) 117, 633, 783, 890, 977
SseBI AGGCCT 1 cut(s) 883
SsiI CCGC 2 cut(s) 211, 767
SspMI CTAG 3 cut(s) 315, 399, 726
StuI AGGCCT 1 cut(s) 883
StyD4I CCNGG 1 cut(s) 539
StyI CCWWGG 1 cut(s) 963
TaaI ACNGT 1 cut(s) 603
TaiI ACGT 5 cut(s) 189, 207, 248, 538, 625
TasI AATT 5 cut(s) 117, 633, 783, 890, 977
TfiI GAWTC 1 cut(s) 41
Tru1I TTAA 4 cut(s) 183, 453, 786, 914
Tru9I TTAA 4 cut(s) 183, 453, 786, 914
TscAI CASTG 2 cut(s) 606, 710
TseI GCWGC 3 cut(s) 113, 521, 869
TspDTI ATGAA 2 cut(s) 506, 633
TspGWI ACGGA 4 cut(s) 26, 104, 258, 988
TspRI CASTG 2 cut(s) 606, 710
VpaK11BI GGWCC 1 cut(s) 266
VspI ATTAAT 1 cut(s) 914
XceI RCATGY 1 cut(s) 901
XmiI GTMKAC 2 cut(s) 207, 957
XspI CTAG 3 cut(s) 315, 399, 726
Zsp2I ATGCAT 1 cut(s) 666
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.