MD02G1204800.v1.1

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0000549
Physical Location & Seq
Reverse (-)
20464736 .. 20464969
234 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1204800.v1.1.491

Sequence Viewer

Length: 234 bp
ATGAGAGAGTGTATCTCCATCCACATTGGCCAGGCCGGAATCCAAATCGGAAACGCCTGCTGGGAGCTCTACCGCCTCGAACACGGCATCCAGCCTGATGGCCAAATGCTGAGTGACAAGACCGTCGATGGTGGAGACGACGCTTTCAACACTTTCTTCAGCGAAACCAGAGTCGGAAAACACATTCCCCGTGCAATGTTCTTGGATCTTGAGCCCGCCCACCGTCATCGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04145 ko04210 ko04530 ko04540 ko05130 map04145 map04210 map04530 map04540 map05130
Pfam Domains
Protein Families
Tubulin

Protein Analysis

78

Amino Acids

8.74

Weight (kDa)

5.88

Isoelectric Point (pI)

34.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tubulin PF00091 3 - 73 2.9e-14 Tubulin/FtsZ family, GTPase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000549)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G04820 AT1G50010 AT4G14960 AT4G14960
fragaria_vesca FvH4_1g18660 FvH4_2g03140 FvH4_5g33560 FvH4_6g48940 FvH4_6g48940
malus_domestica MD02G1204800.v1.1 MD05G1106800.v1.1 MD08G1210600.v1.1 MD09G1049500.v1.1 MD10G1111600.v1.1 MD15G1398700.v1.1 MD17G1049700.v1.1
prunus_persica Prupe.1G542600_v2.0.a1 Prupe.3G269300_v2.0.a1 Prupe.6G212700_v2.0.a1 Prupe.8G150900_v2.0.a1
pyrus_communis pycom05g10370 pycom08g18150 pycom10g09640 pycom10g09660 pycom111g04050 pycom15g35470 pycom17g04460
rosa_chinensis RchiOBHm_Chr2g0109481 RchiOBHm_Chr2g0168751 RchiOBHm_Chr3g0453071 RchiOBHm_Chr3g0460741 RchiOBHm_Chr3g0467971 RchiOBHm_Chr4g0400901 RchiOBHm_Chr6g0248011 RchiOBHm_Chr7g0232381 RchiOBHm_Chr7g0238121
rosa_laevigata RLG00000001350 RLG00000009167 RLG00000015163 RLG00000017708 RLG00000017741 RLG00000021807 RLG00000023849 RLG00000026518
rosa_multiflora Rmu_sc0000332.1_g000021 Rmu_sc0000332.1_g000029 Rmu_sc0002416.1_g000007 Rmu_sc0002611.1_g000001 Rmu_sc0003113.1_g000016 Rmu_sc0004780.1_g000001 Rmu_sc0004805.1_g000035 Rmu_sc0010633.1_g000010 Rmu_sc0011069.1_g000003 Rmu_sc0016170.1_g000002 Rmu_sc0025757.1_g000003 Rmu_ssc0000484.1_g000003
rosa_roxburghii Rroxscaffold_2G00134200 Rroxscaffold_4G00321030 Rroxscaffold_7G00213500
rosa_rugosa Rorug01G0283100 Rorug02G0160500 Rorug02G0535300 Rorug04G0027000 Rorug04G0328100 Rorug05G0388700 Rorug05G0530200 Rorug06G0489200 Rorug07G0104900 Rorug07G0209400 Rorug07G0274500
rosa_samantha Rh2BG222800 Rh2BG618900 Rh2CG214400 Rh2CG587200 Rh6BG040200 Rh6CG206500 Rh6DG035600 Rh7BG401600 Rh7BG401700 Rh7DG418400
rosa_wichuraiana Rw2G050240 Rw6G003920 Rw7G035430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 122
AciI CCGC 2 cut(s) 73, 216
AclWI GGATC 1 cut(s) 213
AcoI YGGCCR 2 cut(s) 28, 100
AcuI CTGAAG 1 cut(s) 142
AgsI TTSAA 1 cut(s) 148
AjnI CCWGG 1 cut(s) 30
AloI GAACNNNNNNTCC 2 cut(s) 72, 104
AluBI AGCT 1 cut(s) 67
AluI AGCT 1 cut(s) 67
Alw21I GWGCWC 1 cut(s) 69
Alw26I GTCTC 1 cut(s) 129
AlwI GGATC 1 cut(s) 213
AoxI GGCC 3 cut(s) 28, 33, 100
ArsI GACNNNNNNTTYG 2 cut(s) 156, 188
BalI TGGCCA 2 cut(s) 30, 102
BanII GRGCYC 2 cut(s) 69, 216
Bbv12I GWGCWC 1 cut(s) 69
BccI CCATC 3 cut(s) 26, 92, 122
BceAI ACGGC 1 cut(s) 100
BciT130I CCWGG 1 cut(s) 32
BcoDI GTCTC 1 cut(s) 129
Bme1390I CCNGG 1 cut(s) 32
BmrFI CCNGG 1 cut(s) 32
BmsI GCATC 1 cut(s) 96
BplI GAGNNNNNCTC 1 cut(s) 31
BpuEI CTTGAG 1 cut(s) 230
Bsa29I ATCGAT 1 cut(s) 229
Bse3DI GCAATG 1 cut(s) 201
BseBI CCWGG 1 cut(s) 32
BseCI ATCGAT 1 cut(s) 229
BseGI GGATG 2 cut(s) 18, 87
BseMI GCAATG 1 cut(s) 201
BseMII CTCAG 1 cut(s) 101
BseYI CCCAGC 1 cut(s) 60
BshFI GGCC 3 cut(s) 30, 35, 102
BshVI ATCGAT 1 cut(s) 229
BsiHKAI GWGCWC 1 cut(s) 69
BsiSI CCGG 1 cut(s) 36
BsmAI GTCTC 1 cut(s) 129
BsmBI CGTCTC 1 cut(s) 129
BsnI GGCC 3 cut(s) 30, 35, 102
Bsp1286I GDGCHC 2 cut(s) 69, 216
Bsp143I GATC 1 cut(s) 205
BspACI CCGC 2 cut(s) 73, 216
BspANI GGCC 3 cut(s) 30, 35, 102
BspCNI CTCAG 1 cut(s) 102
BspDI ATCGAT 1 cut(s) 229
BspPI GGATC 1 cut(s) 213
BsrDI GCAATG 1 cut(s) 201
BssMI GATC 1 cut(s) 205
Bst2UI CCWGG 1 cut(s) 32
Bst4CI ACNGT 2 cut(s) 124, 224
BstC8I GCNNGC 2 cut(s) 58, 216
BstDEI CTNAG 1 cut(s) 110
BstF5I GGATG 2 cut(s) 18, 87
BstKTI GATC 1 cut(s) 208
BstMAI GTCTC 1 cut(s) 129
BstMBI GATC 1 cut(s) 205
BstNI CCWGG 1 cut(s) 32
BstSCI CCNGG 1 cut(s) 30
BstX2I RGATCY 1 cut(s) 205
BstXI CCANNNNNNTGG 1 cut(s) 98
BstYI RGATCY 1 cut(s) 205
Bsu15I ATCGAT 1 cut(s) 229
BsuRI GGCC 3 cut(s) 30, 35, 102
BsuTUI ATCGAT 1 cut(s) 229
BtsCI GGATG 2 cut(s) 18, 87
Cac8I GCNNGC 2 cut(s) 58, 216
ClaI ATCGAT 1 cut(s) 229
CseI GACGC 1 cut(s) 149
CviJI RGCY 6 cut(s) 30, 35, 67, 94, 102, 214
CviKI_1 RGCY 6 cut(s) 30, 35, 67, 94, 102, 214
DdeI CTNAG 1 cut(s) 110
DpnI GATC 1 cut(s) 207
DpnII GATC 1 cut(s) 205
DrdI GACNNNNNNGTC 1 cut(s) 122
DseDI GACNNNNNNGTC 1 cut(s) 122
EaeI YGGCCR 2 cut(s) 28, 100
Ecl136II GAGCTC 1 cut(s) 67
Eco24I GRGCYC 2 cut(s) 69, 216
Eco53kI GAGCTC 1 cut(s) 67
Eco57I CTGAAG 1 cut(s) 142
EcoICRI GAGCTC 1 cut(s) 67
EcoRII CCWGG 1 cut(s) 30
EcoT38I GRGCYC 2 cut(s) 69, 216
Esp3I CGTCTC 1 cut(s) 129
FauI CCCGC 1 cut(s) 223
FokI GGATG 2 cut(s) 5, 74
FriOI GRGCYC 2 cut(s) 69, 216
GsaI CCCAGC 1 cut(s) 64
HaeIII GGCC 3 cut(s) 30, 35, 102
HapII CCGG 1 cut(s) 36
HgaI GACGC 1 cut(s) 149
HinfI GANTC 2 cut(s) 39, 171
HpaII CCGG 1 cut(s) 36
Hpy188I TCNGA 2 cut(s) 50, 176
Hpy188III TCNNGA 1 cut(s) 209
Hpy99I CGWCG 2 cut(s) 128, 143
HpyCH4III ACNGT 2 cut(s) 124, 224
HpyCH4V TGCA 1 cut(s) 194
HpyF3I CTNAG 1 cut(s) 110
Kzo9I GATC 1 cut(s) 205
LmnI GCTCC 1 cut(s) 64
LpnPI CCDG 8 cut(s) 17, 44, 46, 49, 70, 104, 108, 181
LweI GCATC 1 cut(s) 96
MaeIII GTNAC 1 cut(s) 113
MalI GATC 1 cut(s) 207
MboI GATC 1 cut(s) 205
MboII GAAGA 1 cut(s) 148
MflI RGATCY 1 cut(s) 205
MhlI GDGCHC 2 cut(s) 69, 216
MlsI TGGCCA 2 cut(s) 30, 102
MluNI TGGCCA 2 cut(s) 30, 102
MlyI GAGTC 1 cut(s) 180
MmeI TCCRAC 1 cut(s) 154
MnlI CCTC 1 cut(s) 86
Mox20I TGGCCA 2 cut(s) 30, 102
MscI TGGCCA 2 cut(s) 30, 102
Msp20I TGGCCA 2 cut(s) 30, 102
MspI CCGG 1 cut(s) 36
MspR9I CCNGG 1 cut(s) 32
MvaI CCWGG 1 cut(s) 32
NdeII GATC 1 cut(s) 205
NmuCI GTSAC 1 cut(s) 113
PfeI GAWTC 1 cut(s) 39
PleI GAGTC 1 cut(s) 179
PpsI GAGTC 1 cut(s) 179
Psp124BI GAGCTC 1 cut(s) 69
Psp6I CCWGG 1 cut(s) 30
PspFI CCCAGC 1 cut(s) 60
PspGI CCWGG 1 cut(s) 30
PsuI RGATCY 1 cut(s) 205
SacI GAGCTC 1 cut(s) 69
Sau3AI GATC 1 cut(s) 205
SchI GAGTC 1 cut(s) 180
ScrFI CCNGG 1 cut(s) 32
SduI GDGCHC 2 cut(s) 69, 216
SetI ASST 1 cut(s) 69
SfaNI GCATC 1 cut(s) 96
SmlI CTYRAG 1 cut(s) 209
SmoI CTYRAG 1 cut(s) 209
SsiI CCGC 2 cut(s) 73, 216
SstI GAGCTC 1 cut(s) 69
StyD4I CCNGG 1 cut(s) 30
TaaI ACNGT 2 cut(s) 124, 224
TaqI TCGA 3 cut(s) 78, 126, 229
TfiI GAWTC 1 cut(s) 39
TseFI GTSAC 1 cut(s) 113
Tsp45I GTSAC 1 cut(s) 113
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.