RLG00000017708

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0000549
Physical Location & Seq
Forward (+)
20260077 .. 20260617
541 bp
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UTR
Exon/CDS
Intron
RLM00000017708

Sequence Viewer

Length: 435 bp
ATGCCAAGCGACAAGACCATTGATCGCGGCGACGACGCCTTCAACACCTTCTTCTCCGAGACCGGCGCCGGCAAGCACGACCCACGCCCCATCTTCGTCGATCTCGAGCCCACCGTTATCGATGAGGTCAGCACCGAAACCTACCACAAGCTCTTCCAGCTCGAGCAACTCATCTCCGGCAAGGAGGACGCTGCCAACAACTTCGCCTGTGGTCACTACACCATCGTCAAAGAGATCGTCGATCTCTGCCTGGATCAGATTCGGAAGCTCGCTGATAACTGCACCGAGCTCCAGGGATCCCTGGTCTTCAATGCCGTCGGTGGCGATACCGGGTCGGGTCTCGAGTCGCTTCTGCTGGAGAGGCTTTCTGTGGACTATGGGAAAAAGTCCAAGCTCGGATTCACTGTGTATCCTTCTCCTCAGATGTATTTATAG

Protein Analysis

145

Amino Acids

15.91

Weight (kDa)

4.6

Isoelectric Point (pI)

32.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tubulin PF00091 13 - 139 3.2e-29 Tubulin/FtsZ family, GTPase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000549)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G04820 AT1G50010 AT4G14960 AT4G14960
fragaria_vesca FvH4_1g18660 FvH4_2g03140 FvH4_5g33560 FvH4_6g48940 FvH4_6g48940
malus_domestica MD02G1204800.v1.1 MD05G1106800.v1.1 MD08G1210600.v1.1 MD09G1049500.v1.1 MD10G1111600.v1.1 MD15G1398700.v1.1 MD17G1049700.v1.1
prunus_persica Prupe.1G542600_v2.0.a1 Prupe.3G269300_v2.0.a1 Prupe.6G212700_v2.0.a1 Prupe.8G150900_v2.0.a1
pyrus_communis pycom05g10370 pycom08g18150 pycom10g09640 pycom10g09660 pycom111g04050 pycom15g35470 pycom17g04460
rosa_chinensis RchiOBHm_Chr2g0109481 RchiOBHm_Chr2g0168751 RchiOBHm_Chr3g0453071 RchiOBHm_Chr3g0460741 RchiOBHm_Chr3g0467971 RchiOBHm_Chr4g0400901 RchiOBHm_Chr6g0248011 RchiOBHm_Chr7g0232381 RchiOBHm_Chr7g0238121
rosa_laevigata RLG00000001350 RLG00000009167 RLG00000015163 RLG00000017708 RLG00000017741 RLG00000021807 RLG00000023849 RLG00000026518
rosa_multiflora Rmu_sc0000332.1_g000021 Rmu_sc0000332.1_g000029 Rmu_sc0002416.1_g000007 Rmu_sc0002611.1_g000001 Rmu_sc0003113.1_g000016 Rmu_sc0004780.1_g000001 Rmu_sc0004805.1_g000035 Rmu_sc0010633.1_g000010 Rmu_sc0011069.1_g000003 Rmu_sc0016170.1_g000002 Rmu_sc0025757.1_g000003 Rmu_ssc0000484.1_g000003
rosa_roxburghii Rroxscaffold_2G00134200 Rroxscaffold_4G00321030 Rroxscaffold_7G00213500
rosa_rugosa Rorug01G0283100 Rorug02G0160500 Rorug02G0535300 Rorug04G0027000 Rorug04G0328100 Rorug05G0388700 Rorug05G0530200 Rorug06G0489200 Rorug07G0104900 Rorug07G0209400 Rorug07G0274500
rosa_samantha Rh2BG222800 Rh2BG618900 Rh2CG214400 Rh2CG587200 Rh6BG040200 Rh6CG206500 Rh6DG035600 Rh7BG401600 Rh7BG401700 Rh7DG418400
rosa_wichuraiana Rw2G050240 Rw6G003920 Rw7G035430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 65
AccII CGCG 1 cut(s) 27
AciI CCGC 1 cut(s) 27
AclWI GGATC 3 cut(s) 261, 291, 304
AcyI GRCGYC 2 cut(s) 36, 66
AgsI TTSAA 2 cut(s) 43, 310
AjnI CCWGG 3 cut(s) 249, 291, 300
AjuI GAANNNNNNNTTGG 2 cut(s) 383, 415
AluBI AGCT 5 cut(s) 151, 160, 268, 289, 394
AluI AGCT 5 cut(s) 151, 160, 268, 289, 394
Alw21I GWGCWC 1 cut(s) 291
Alw26I GTCTC 2 cut(s) 53, 344
AlwI GGATC 3 cut(s) 261, 291, 304
Ama87I CYCGRG 3 cut(s) 104, 161, 341
ApeKI GCWGC 1 cut(s) 191
ArsI GACNNNNNNTTYG 2 cut(s) 222, 254
AspLEI GCGC 1 cut(s) 68
AsuC2I CCSGG 1 cut(s) 331
AvaI CYCGRG 3 cut(s) 104, 161, 341
BamHI GGATCC 1 cut(s) 296
BanI GGYRCC 1 cut(s) 65
BanII GRGCYC 2 cut(s) 111, 291
BbsI GAAGAC 1 cut(s) 298
Bbv12I GWGCWC 1 cut(s) 291
BbvI GCAGC 1 cut(s) 178
BccI CCATC 2 cut(s) 98, 230
BceAI ACGGC 1 cut(s) 299
BciT130I CCWGG 3 cut(s) 251, 293, 302
BciVI GTATCC 1 cut(s) 420
BcnI CCSGG 1 cut(s) 331
BcoDI GTCTC 2 cut(s) 53, 344
BfoI RGCGCY 1 cut(s) 69
BfuI GTATCC 1 cut(s) 420
BisI GCNGC 2 cut(s) 28, 192
BlsI GCNGC 2 cut(s) 29, 193
Bme1390I CCNGG 4 cut(s) 251, 293, 302, 331
BmeT110I CYCGRG 3 cut(s) 104, 161, 341
BmiI GGNNCC 2 cut(s) 67, 298
BmrFI CCNGG 4 cut(s) 251, 293, 302, 331
BpiI GAAGAC 1 cut(s) 298
BpmI CTGGAG 2 cut(s) 275, 377
BpuMI CCSGG 1 cut(s) 331
Bsa29I ATCGAT 1 cut(s) 120
BsaHI GRCGYC 2 cut(s) 36, 66
BsaI GGTCTC 2 cut(s) 53, 344
BsaJI CCNNGG 2 cut(s) 292, 300
Bse118I RCCGGY 2 cut(s) 62, 68
BseBI CCWGG 3 cut(s) 251, 293, 302
BseCI ATCGAT 1 cut(s) 120
BseDI CCNNGG 2 cut(s) 292, 300
BseMII CTCAG 1 cut(s) 434
BseRI GAGGAG 1 cut(s) 408
BseXI GCAGC 1 cut(s) 178
BsgI GTGCAG 1 cut(s) 265
Bsh1236I CGCG 1 cut(s) 27
BshNI GGYRCC 1 cut(s) 65
BshVI ATCGAT 1 cut(s) 120
BsiHKAI GWGCWC 1 cut(s) 291
BsiHKCI CYCGRG 3 cut(s) 104, 161, 341
BsiSI CCGG 4 cut(s) 63, 69, 177, 330
BsmAI GTCTC 2 cut(s) 53, 344
Bso31I GGTCTC 2 cut(s) 53, 344
BsoBI CYCGRG 3 cut(s) 104, 161, 341
Bsp1286I GDGCHC 2 cut(s) 111, 291
Bsp143I GATC 6 cut(s) 22, 100, 234, 241, 253, 296
BspACI CCGC 1 cut(s) 27
BspCNI CTCAG 1 cut(s) 433
BspDI ATCGAT 1 cut(s) 120
BspFNI CGCG 1 cut(s) 27
BspLI GGNNCC 2 cut(s) 67, 298
BspPI GGATC 3 cut(s) 261, 291, 304
BspQI GCTCTTC 1 cut(s) 158
BspT107I GGYRCC 1 cut(s) 65
BspTNI GGTCTC 2 cut(s) 53, 344
BsrFI RCCGGY 2 cut(s) 62, 68
BssAI RCCGGY 2 cut(s) 62, 68
BssECI CCNNGG 2 cut(s) 292, 300
BssMI GATC 6 cut(s) 22, 100, 234, 241, 253, 296
BssNI GRCGYC 2 cut(s) 36, 66
Bst2UI CCWGG 3 cut(s) 251, 293, 302
Bst4CI ACNGT 2 cut(s) 115, 406
Bst6I CTCTTC 1 cut(s) 158
BstACI GRCGYC 2 cut(s) 36, 66
BstC8I GCNNGC 3 cut(s) 70, 74, 270
BstDEI CTNAG 1 cut(s) 420
BstFNI CGCG 1 cut(s) 27
BstH2I RGCGCY 1 cut(s) 69
BstHHI GCGC 1 cut(s) 68
BstKTI GATC 6 cut(s) 25, 103, 237, 244, 256, 299
BstMAI GTCTC 2 cut(s) 53, 344
BstMBI GATC 6 cut(s) 22, 100, 234, 241, 253, 296
BstMWI GCNNNNNNNGC 2 cut(s) 157, 361
BstNI CCWGG 3 cut(s) 251, 293, 302
BstSCI CCNGG 4 cut(s) 249, 291, 300, 329
BstUI CGCG 1 cut(s) 27
BstV1I GCAGC 1 cut(s) 178
BstV2I GAAGAC 1 cut(s) 298
BstX2I RGATCY 1 cut(s) 296
BstYI RGATCY 1 cut(s) 296
Bsu15I ATCGAT 1 cut(s) 120
BsuI GTATCC 1 cut(s) 420
BsuTUI ATCGAT 1 cut(s) 120
BtsIMutI CAGTG 1 cut(s) 402
Cac8I GCNNGC 3 cut(s) 70, 74, 270
CfoI GCGC 1 cut(s) 68
Cfr10I RCCGGY 2 cut(s) 62, 68
ClaI ATCGAT 1 cut(s) 120
CseI GACGC 2 cut(s) 44, 197
CviJI RGCY 7 cut(s) 109, 151, 160, 268, 289, 364, 394
CviKI_1 RGCY 7 cut(s) 109, 151, 160, 268, 289, 364, 394
DdeI CTNAG 1 cut(s) 420
DinI GGCGCC 1 cut(s) 67
DpnI GATC 6 cut(s) 24, 102, 236, 243, 255, 298
DpnII GATC 6 cut(s) 22, 100, 234, 241, 253, 296
Eam1104I CTCTTC 1 cut(s) 158
EarI CTCTTC 1 cut(s) 158
Ecl136II GAGCTC 1 cut(s) 289
Eco24I GRGCYC 2 cut(s) 111, 291
Eco31I GGTCTC 2 cut(s) 53, 344
Eco53kI GAGCTC 1 cut(s) 289
Eco88I CYCGRG 3 cut(s) 104, 161, 341
EcoICRI GAGCTC 1 cut(s) 289
EcoRII CCWGG 3 cut(s) 249, 291, 300
EcoT38I GRGCYC 2 cut(s) 111, 291
EgeI GGCGCC 1 cut(s) 67
EheI GGCGCC 1 cut(s) 67
FaiI YATR 2 cut(s) 378, 433
Fnu4HI GCNGC 2 cut(s) 28, 192
FriOI GRGCYC 2 cut(s) 111, 291
Fsp4HI GCNGC 2 cut(s) 28, 192
GlaI GCGC 1 cut(s) 67
GluI GCNGC 2 cut(s) 28, 192
GsuI CTGGAG 2 cut(s) 275, 377
HaeII RGCGCY 1 cut(s) 69
HapII CCGG 4 cut(s) 63, 69, 177, 330
HgaI GACGC 2 cut(s) 44, 197
HhaI GCGC 1 cut(s) 68
Hin1I GRCGYC 2 cut(s) 36, 66
Hin6I GCGC 1 cut(s) 66
HinP1I GCGC 1 cut(s) 66
HinfI GANTC 3 cut(s) 259, 344, 399
HpaII CCGG 4 cut(s) 63, 69, 177, 330
Hpy166II GTNNAC 1 cut(s) 373
Hpy188I TCNGA 5 cut(s) 58, 258, 264, 398, 423
Hpy188III TCNNGA 2 cut(s) 104, 341
Hpy8I GTNNAC 1 cut(s) 373
Hpy99I CGWCG 5 cut(s) 35, 38, 101, 242, 320
HpyAV CCTTC 3 cut(s) 49, 58, 423
HpyCH4III ACNGT 2 cut(s) 115, 406
HpyCH4V TGCA 1 cut(s) 282
HpyF10VI GCNNNNNNNGC 2 cut(s) 157, 361
HpyF3I CTNAG 1 cut(s) 420
Hsp92I GRCGYC 2 cut(s) 36, 66
HspAI GCGC 1 cut(s) 66
KasI GGCGCC 1 cut(s) 65
KroI GCCGGC 1 cut(s) 68
KroNI GCCGGC 1 cut(s) 70
Kzo9I GATC 6 cut(s) 22, 100, 234, 241, 253, 296
LguI GCTCTTC 1 cut(s) 158
LmnI GCTCC 1 cut(s) 294
Lsp1109I GCAGC 1 cut(s) 178
MaeIII GTNAC 1 cut(s) 212
MalI GATC 6 cut(s) 24, 102, 236, 243, 255, 298
MboI GATC 6 cut(s) 22, 100, 234, 241, 253, 296
MboII GAAGA 4 cut(s) 43, 85, 145, 298
MflI RGATCY 1 cut(s) 296
MhlI GDGCHC 2 cut(s) 111, 291
Mly113I GGCGCC 1 cut(s) 66
MlyI GAGTC 1 cut(s) 353
MnlI CCTC 4 cut(s) 118, 178, 354, 429
MroNI GCCGGC 1 cut(s) 68
MspI CCGG 4 cut(s) 63, 69, 177, 330
MspR9I CCNGG 4 cut(s) 251, 293, 302, 331
MvaI CCWGG 3 cut(s) 251, 293, 302
MvnI CGCG 1 cut(s) 27
MwoI GCNNNNNNNGC 2 cut(s) 157, 361
NaeI GCCGGC 1 cut(s) 70
NarI GGCGCC 1 cut(s) 66
NciI CCSGG 1 cut(s) 331
NdeII GATC 6 cut(s) 22, 100, 234, 241, 253, 296
NgoMIV GCCGGC 1 cut(s) 68
NlaIV GGNNCC 2 cut(s) 67, 298
NmuCI GTSAC 1 cut(s) 212
PaeR7I CTCGAG 3 cut(s) 104, 161, 341
PciSI GCTCTTC 1 cut(s) 158
PcsI WCGNNNNNNNCGW 2 cut(s) 102, 111
PdiI GCCGGC 1 cut(s) 70
PfeI GAWTC 2 cut(s) 259, 399
PkrI GCNGC 2 cut(s) 29, 193
PleI GAGTC 1 cut(s) 352
PluTI GGCGCC 1 cut(s) 69
PpsI GAGTC 1 cut(s) 352
Psp124BI GAGCTC 1 cut(s) 291
Psp6I CCWGG 3 cut(s) 249, 291, 300
PspGI CCWGG 3 cut(s) 249, 291, 300
PspN4I GGNNCC 2 cut(s) 67, 298
PspXI VCTCGAGB 1 cut(s) 161
PsuI RGATCY 1 cut(s) 296
SacI GAGCTC 1 cut(s) 291
SapI GCTCTTC 1 cut(s) 158
SatI GCNGC 2 cut(s) 28, 192
Sau3AI GATC 6 cut(s) 22, 100, 234, 241, 253, 296
SchI GAGTC 1 cut(s) 353
ScrFI CCNGG 4 cut(s) 251, 293, 302, 331
SduI GDGCHC 2 cut(s) 111, 291
SetI ASST 8 cut(s) 50, 129, 143, 153, 162, 270, 291, 396
SfoI GGCGCC 1 cut(s) 67
Sfr274I CTCGAG 3 cut(s) 104, 161, 341
SlaI CTCGAG 3 cut(s) 104, 161, 341
SmlI CTYRAG 3 cut(s) 104, 161, 341
SmoI CTYRAG 3 cut(s) 104, 161, 341
SsiI CCGC 1 cut(s) 27
SspDI GGCGCC 1 cut(s) 65
SstI GAGCTC 1 cut(s) 291
StyD4I CCNGG 4 cut(s) 249, 291, 300, 329
TaaI ACNGT 2 cut(s) 115, 406
TaqI TCGA 6 cut(s) 99, 105, 120, 162, 240, 342
TauI GCSGC 1 cut(s) 30
TfiI GAWTC 2 cut(s) 259, 399
TscAI CASTG 1 cut(s) 409
TseFI GTSAC 1 cut(s) 212
TseI GCWGC 1 cut(s) 191
Tsp45I GTSAC 1 cut(s) 212
TspRI CASTG 1 cut(s) 409
XhoI CTCGAG 3 cut(s) 104, 161, 341
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.