Rh2BG222800

Tubulin/FtsZ family, C-terminal domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0000549
Physical Location & Seq
Reverse (-)
21547094 .. 21550266
3173 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG222800.1

Sequence Viewer

Length: 1251 bp
ATGCCAAGTGACAAGACCGTTGGTGGAGGTGATGATGCTTTCAACACCTTCTTTAGTGAGACTGGTGCTGGGAAGCATGTGCCTCGTGCAGTGTTTGTAGATTTGGAGCCCACTGTCATTGATGAAGTGAGGACTGGAACATACCGCCAACTCTTCCACCCTGAACAGCTCATCAGTGGCAAGGAAGACGCAGCCAACAACTTTGCTCGTGGCCACTATACCATTGGGAAAGAGATTGTTGACCTGTGCCTGGACCGTATTCGAAAGCTTGCTGACAACTGCACTGGTCTCCAAGGGTTCCTTGTTTTCAATGCTGTGGGTGGAGGCACTGGTTCAGGTCTTGGCTCCCTTTTACTGGAACGTCTCTCAGTGGACTATGGCAAGAAGTCCAAGCTTGGATTCACTGTCTACCCCTCTCCACAAGTATCTACCTCTGTGGTTGAGCCCTACAACAGTGTTCTCTCAACCCACTCCCTTCTGGAACACACTGATGTTGCTGTGCTGTTGGATAATGAGGCCATCTATGACATATGCAGGCGCTCCCTTGACATTGAACGCCCTACCTATACCAACCTCAACCGCCTCGTTTCACAGGTTATCTCATCTCTGACTGCATCTCTGAGGTTTGATGGTGCCTTGAATGTGGATGTGACTGAATTCCAGACCAATCTTGTCCCATACCCCAGAATCCACTTCATGCTTTCATCTTATGCTCCTGTTATCTCAGCTGAGAAGGCATATCATGAGCAGCTTTCAGTTGCAGAGATCACCAACAGCGCATTTGAGCCTTCCTCCATGATGGCCAAGTGCGATCCTCGCCATGGTAAATACATGGCCTGCTGTCTCATGTACAGAGGTGATGTGGTGCCCAAGGATGTCAACGCAGCTGTTGCCACCATCAAGACCAAGCGTACCATCCAATTTGTTGACTGGTGCCCCACTGGATTCAAATGTGGTATCAACTACCAGCCCCCAACTGTTGTTCCAGGTGGTGATCTCGCCAAGGTTCAGAGGGCAGTGTGCATGATCTCAAACTCCACCAGCGTTGCTGAGGTGTTCTCACGCATTGACCACAAGTTTGACCTCATGTATGCCAAGAGGGCCTTCGTCCACTGGTATGTCGGTGAGGGAATGGAGGAAGGTGAGTTCTCTGAGGCCCGTGAGGATCTTGCTGCCCTGGAGAAGGACTATGAGGAGGTTGGTGCTGAGGGCGAGGAGGGCGAAGACGGTGATGAGGGAGATGAGTATTGA

Protein Analysis

416

Amino Acids

45.8

Weight (kDa)

4.9

Isoelectric Point (pI)

35.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tubulin PF00091 13 - 178 1.4e-50 Tubulin/FtsZ family, GTPase domain
Tubulin_C PF03953 228 - 357 3.9e-52 Tubulin C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000549)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G04820 AT1G50010 AT4G14960 AT4G14960
fragaria_vesca FvH4_1g18660 FvH4_2g03140 FvH4_5g33560 FvH4_6g48940 FvH4_6g48940
malus_domestica MD02G1204800.v1.1 MD05G1106800.v1.1 MD08G1210600.v1.1 MD09G1049500.v1.1 MD10G1111600.v1.1 MD15G1398700.v1.1 MD17G1049700.v1.1
prunus_persica Prupe.1G542600_v2.0.a1 Prupe.3G269300_v2.0.a1 Prupe.6G212700_v2.0.a1 Prupe.8G150900_v2.0.a1
pyrus_communis pycom05g10370 pycom08g18150 pycom10g09640 pycom10g09660 pycom111g04050 pycom15g35470 pycom17g04460
rosa_chinensis RchiOBHm_Chr2g0109481 RchiOBHm_Chr2g0168751 RchiOBHm_Chr3g0453071 RchiOBHm_Chr3g0460741 RchiOBHm_Chr3g0467971 RchiOBHm_Chr4g0400901 RchiOBHm_Chr6g0248011 RchiOBHm_Chr7g0232381 RchiOBHm_Chr7g0238121
rosa_laevigata RLG00000001350 RLG00000009167 RLG00000015163 RLG00000017708 RLG00000017741 RLG00000021807 RLG00000023849 RLG00000026518
rosa_multiflora Rmu_sc0000332.1_g000021 Rmu_sc0000332.1_g000029 Rmu_sc0002416.1_g000007 Rmu_sc0002611.1_g000001 Rmu_sc0003113.1_g000016 Rmu_sc0004780.1_g000001 Rmu_sc0004805.1_g000035 Rmu_sc0010633.1_g000010 Rmu_sc0011069.1_g000003 Rmu_sc0016170.1_g000002 Rmu_sc0025757.1_g000003 Rmu_ssc0000484.1_g000003
rosa_roxburghii Rroxscaffold_2G00134200 Rroxscaffold_4G00321030 Rroxscaffold_7G00213500
rosa_rugosa Rorug01G0283100 Rorug02G0160500 Rorug02G0535300 Rorug04G0027000 Rorug04G0328100 Rorug05G0388700 Rorug05G0530200 Rorug06G0489200 Rorug07G0104900 Rorug07G0209400 Rorug07G0274500
rosa_samantha Rh2BG222800 Rh2BG618900 Rh2CG214400 Rh2CG587200 Rh6BG040200 Rh6CG206500 Rh6DG035600 Rh7BG401600 Rh7BG401700 Rh7DG418400
rosa_wichuraiana Rw2G050240 Rw6G003920 Rw7G035430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 3 cut(s) 632, 865, 933
AccI GTMKAC 1 cut(s) 408
AciI CCGC 2 cut(s) 145, 580
AclWI GGATC 2 cut(s) 806, 1173
AcoI YGGCCR 2 cut(s) 211, 801
AcsI RAATTY 1 cut(s) 656
AfaI GTAC 2 cut(s) 851, 913
AfiI CCNNNNNNNGG 4 cut(s) 250, 355, 821, 1183
AgsI TTSAA 5 cut(s) 43, 310, 554, 640, 949
AjnI CCWGG 3 cut(s) 249, 985, 1176
AjuI GAANNNNNNNTTGG 4 cut(s) 383, 415, 1088, 1120
AloI GAACNNNNNNTCC 2 cut(s) 1130, 1162
AluBI AGCT 6 cut(s) 169, 268, 394, 728, 751, 887
AluI AGCT 6 cut(s) 169, 268, 394, 728, 751, 887
Alw26I GTCTC 4 cut(s) 53, 293, 368, 848
AlwI GGATC 2 cut(s) 806, 1173
AoxI GGCC 6 cut(s) 211, 516, 801, 834, 1101, 1155
ApeKI GCWGC 4 cut(s) 191, 748, 884, 1172
ApoI RAATTY 1 cut(s) 656
ArsI GACNNNNNNTTYG 2 cut(s) 1061, 1093
AspLEI GCGC 2 cut(s) 540, 779
AspS9I GGNCC 3 cut(s) 253, 1101, 1156
AsuHPI GGTGA 7 cut(s) 41, 760, 869, 1004, 1136, 1154, 1241
AsuII TTCGAA 1 cut(s) 262
AvaII GGWCC 1 cut(s) 253
BaeGI GKGCMC 2 cut(s) 870, 938
BalI TGGCCA 2 cut(s) 213, 803
BanI GGYRCC 3 cut(s) 632, 865, 933
BanII GRGCYC 2 cut(s) 111, 447
BauI CACGAG 2 cut(s) 84, 207
BbsI GAAGAC 2 cut(s) 192, 1230
BbvCI CCTCAGC 2 cut(s) 1050, 1206
BbvI GCAGC 4 cut(s) 203, 760, 896, 1159
BccI CCATC 5 cut(s) 527, 623, 793, 905, 923
BcgI CGANNNNNNTGC 2 cut(s) 65, 99
BciT130I CCWGG 3 cut(s) 251, 987, 1178
BcoDI GTCTC 4 cut(s) 53, 293, 368, 848
BfoI RGCGCY 1 cut(s) 541
BglI GCCNNNNNGGC 1 cut(s) 1100
BisI GCNGC 4 cut(s) 192, 749, 885, 1173
BlsI GCNGC 4 cut(s) 193, 750, 886, 1174
Bme1390I CCNGG 3 cut(s) 251, 987, 1178
Bme18I GGWCC 1 cut(s) 253
BmgT120I GGNCC 3 cut(s) 253, 1101, 1156
BmiI GGNNCC 6 cut(s) 108, 299, 346, 634, 867, 935
BmrFI CCNGG 3 cut(s) 251, 987, 1178
BmsI GCATC 2 cut(s) 25, 623
BpiI GAAGAC 2 cut(s) 192, 1230
BplI GAGNNNNNCTC 4 cut(s) 776, 808, 1043, 1075
BpmI CTGGAG 1 cut(s) 1199
Bpu10I CCTNAGC 2 cut(s) 1050, 1206
Bpu14I TTCGAA 1 cut(s) 262
BsaI GGTCTC 1 cut(s) 293
BsaJI CCNNGG 5 cut(s) 292, 820, 870, 1002, 1176
Bsc4I CCNNNNNNNGG 4 cut(s) 250, 355, 821, 1183
Bse1I ACTGG 8 cut(s) 67, 139, 289, 334, 360, 935, 946, 1118
BseBI CCWGG 3 cut(s) 251, 987, 1178
BseDI CCNNGG 5 cut(s) 292, 820, 870, 1002, 1176
BseGI GGATG 3 cut(s) 652, 880, 915
BseLI CCNNNNNNNGG 4 cut(s) 250, 355, 821, 1183
BseMII CTCAG 7 cut(s) 381, 611, 720, 738, 1041, 1143, 1197
BseNI ACTGG 8 cut(s) 67, 139, 289, 334, 360, 935, 946, 1118
BseRI GAGGAG 2 cut(s) 1208, 1229
BseSI GKGCMC 2 cut(s) 870, 938
BseXI GCAGC 4 cut(s) 203, 760, 896, 1159
BseYI CCCAGC 1 cut(s) 68
BsgI GTGCAG 2 cut(s) 108, 265
BshFI GGCC 6 cut(s) 213, 518, 803, 836, 1103, 1157
BshNI GGYRCC 3 cut(s) 632, 865, 933
BslFI GGGAC 1 cut(s) 659
BslI CCNNNNNNNGG 4 cut(s) 250, 355, 821, 1183
BsmAI GTCTC 4 cut(s) 53, 293, 368, 848
BsmBI CGTCTC 1 cut(s) 368
BsmFI GGGAC 1 cut(s) 659
BsnI GGCC 6 cut(s) 213, 518, 803, 836, 1103, 1157
Bso31I GGTCTC 1 cut(s) 293
Bsp119I TTCGAA 1 cut(s) 262
Bsp1286I GDGCHC 4 cut(s) 111, 447, 870, 938
Bsp1407I TGTACA 1 cut(s) 849
Bsp143I GATC 5 cut(s) 765, 811, 994, 1026, 1165
Bsp19I CCATGG 1 cut(s) 820
BspACI CCGC 2 cut(s) 145, 580
BspANI GGCC 6 cut(s) 213, 518, 803, 836, 1103, 1157
BspCNI CTCAG 7 cut(s) 380, 612, 721, 737, 1042, 1144, 1198
BspHI TCATGA 1 cut(s) 742
BspLI GGNNCC 6 cut(s) 108, 299, 346, 634, 867, 935
BspPI GGATC 2 cut(s) 806, 1173
BspT104I TTCGAA 1 cut(s) 262
BspT107I GGYRCC 3 cut(s) 632, 865, 933
BspTNI GGTCTC 1 cut(s) 293
BsrGI TGTACA 1 cut(s) 849
BsrI ACTGG 8 cut(s) 67, 139, 289, 334, 360, 935, 946, 1118
BssECI CCNNGG 5 cut(s) 292, 820, 870, 1002, 1176
BssMI GATC 5 cut(s) 765, 811, 994, 1026, 1165
BssSI CACGAG 2 cut(s) 84, 207
BssT1I CCWWGG 4 cut(s) 292, 820, 870, 1002
Bst2BI CACGAG 2 cut(s) 84, 207
Bst2UI CCWGG 3 cut(s) 251, 987, 1178
Bst4CI ACNGT 7 cut(s) 19, 115, 257, 406, 455, 979, 1229
Bst6I CTCTTC 1 cut(s) 158
BstAPI GCANNNNNTGC 1 cut(s) 890
BstAUI TGTACA 1 cut(s) 849
BstBI TTCGAA 1 cut(s) 262
BstC8I GCNNGC 3 cut(s) 270, 536, 838
BstDEI CTNAG 7 cut(s) 367, 620, 724, 729, 1050, 1152, 1206
BstDSI CCRYGG 1 cut(s) 820
BstENI CCTNNNNNAGG 1 cut(s) 1181
BstF5I GGATG 3 cut(s) 652, 880, 915
BstH2I RGCGCY 1 cut(s) 541
BstHHI GCGC 2 cut(s) 540, 779
BstKTI GATC 5 cut(s) 768, 814, 997, 1029, 1168
BstMAI GTCTC 4 cut(s) 53, 293, 368, 848
BstMBI GATC 5 cut(s) 765, 811, 994, 1026, 1165
BstMWI GCNNNNNNNGC 5 cut(s) 734, 816, 890, 1100, 1218
BstNI CCWGG 3 cut(s) 251, 987, 1178
BstNSI RCATGY 1 cut(s) 80
BstSCI CCNGG 3 cut(s) 249, 985, 1176
BstSLI GKGCMC 2 cut(s) 870, 938
BstV1I GCAGC 4 cut(s) 203, 760, 896, 1159
BstV2I GAAGAC 2 cut(s) 192, 1230
BstX2I RGATCY 1 cut(s) 1165
BstYI RGATCY 1 cut(s) 1165
BsuRI GGCC 6 cut(s) 213, 518, 803, 836, 1103, 1157
BtgI CCRYGG 1 cut(s) 820
BtsCI GGATG 3 cut(s) 652, 880, 915
BtsI GCAGTG 2 cut(s) 96, 1023
Cac8I GCNNGC 3 cut(s) 270, 536, 838
CciI TCATGA 1 cut(s) 742
CfoI GCGC 2 cut(s) 540, 779
Cfr13I GGNCC 3 cut(s) 253, 1101, 1156
CseI GACGC 1 cut(s) 197
Csp6I GTAC 2 cut(s) 850, 912
CspCI CAANNNNNGTGG 4 cut(s) 100, 135, 1027, 1062
CviAII CATG 9 cut(s) 77, 697, 743, 796, 821, 832, 847, 1024, 1087
CviQI GTAC 2 cut(s) 850, 912
DdeI CTNAG 7 cut(s) 367, 620, 724, 729, 1050, 1152, 1206
DpnI GATC 5 cut(s) 767, 813, 996, 1028, 1167
DpnII GATC 5 cut(s) 765, 811, 994, 1026, 1165
EaeI YGGCCR 2 cut(s) 211, 801
Eam1104I CTCTTC 1 cut(s) 158
EarI CTCTTC 1 cut(s) 158
Eco130I CCWWGG 4 cut(s) 292, 820, 870, 1002
Eco24I GRGCYC 2 cut(s) 111, 447
Eco31I GGTCTC 1 cut(s) 293
Eco47I GGWCC 1 cut(s) 253
EcoNI CCTNNNNNAGG 1 cut(s) 1181
EcoO109I RGGNCCY 1 cut(s) 1101
EcoRI GAATTC 1 cut(s) 656
EcoRII CCWGG 3 cut(s) 249, 985, 1176
EcoT14I CCWWGG 4 cut(s) 292, 820, 870, 1002
EcoT38I GRGCYC 2 cut(s) 111, 447
ErhI CCWWGG 4 cut(s) 292, 820, 870, 1002
Esp3I CGTCTC 1 cut(s) 368
FaeI CATG 9 cut(s) 80, 700, 746, 799, 824, 835, 850, 1027, 1090
FalI AAGNNNNNCTT 4 cut(s) 285, 317, 1088, 1120
FaqI GGGAC 1 cut(s) 659
FatI CATG 9 cut(s) 76, 696, 742, 795, 820, 831, 846, 1023, 1086
FauNDI CATATG 1 cut(s) 530
FblI GTMKAC 1 cut(s) 408
Fnu4HI GCNGC 4 cut(s) 192, 749, 885, 1173
FokI GGATG 3 cut(s) 659, 887, 902
FriOI GRGCYC 2 cut(s) 111, 447
Fsp4HI GCNGC 4 cut(s) 192, 749, 885, 1173
GlaI GCGC 2 cut(s) 539, 778
GluI GCNGC 4 cut(s) 192, 749, 885, 1173
GsaI CCCAGC 1 cut(s) 72
GsuI CTGGAG 1 cut(s) 1199
HaeII RGCGCY 1 cut(s) 541
HaeIII GGCC 6 cut(s) 213, 518, 803, 836, 1103, 1157
HgaI GACGC 1 cut(s) 197
HhaI GCGC 2 cut(s) 540, 779
Hin1II CATG 9 cut(s) 80, 700, 746, 799, 824, 835, 850, 1027, 1090
Hin6I GCGC 2 cut(s) 538, 777
HinP1I GCGC 2 cut(s) 538, 777
HincII GTYRAC 3 cut(s) 241, 880, 928
HindII GTYRAC 3 cut(s) 241, 880, 928
HindIII AAGCTT 2 cut(s) 266, 392
HinfI GANTC 3 cut(s) 399, 687, 945
HphI GGTGA 7 cut(s) 41, 760, 869, 1004, 1136, 1154, 1241
Hpy166II GTNNAC 6 cut(s) 241, 373, 409, 880, 928, 1111
Hpy188I TCNGA 4 cut(s) 609, 621, 1011, 1153
Hpy188III TCNNGA 4 cut(s) 479, 661, 743, 901
Hpy8I GTNNAC 6 cut(s) 241, 373, 409, 880, 928, 1111
HpyAV CCTTC 7 cut(s) 58, 485, 727, 798, 1114, 1133, 1177
HpyCH4III ACNGT 7 cut(s) 19, 115, 257, 406, 455, 979, 1229
HpyCH4IV ACGT 1 cut(s) 361
HpyCH4V TGCA 6 cut(s) 89, 282, 534, 614, 761, 1023
HpyF10VI GCNNNNNNNGC 5 cut(s) 734, 816, 890, 1100, 1218
HpyF3I CTNAG 7 cut(s) 367, 620, 724, 729, 1050, 1152, 1206
HpySE526I ACGT 1 cut(s) 361
Hsp92II CATG 9 cut(s) 80, 700, 746, 799, 824, 835, 850, 1027, 1090
HspAI GCGC 2 cut(s) 538, 777
Kzo9I GATC 5 cut(s) 765, 811, 994, 1026, 1165
LmnI GCTCC 4 cut(s) 106, 350, 545, 718
Lsp1109I GCAGC 4 cut(s) 203, 760, 896, 1159
LweI GCATC 2 cut(s) 25, 623
MaeII ACGT 1 cut(s) 361
MaeIII GTNAC 2 cut(s) 8, 649
MalI GATC 5 cut(s) 767, 813, 996, 1028, 1167
MboI GATC 5 cut(s) 765, 811, 994, 1026, 1165
MboII GAAGA 3 cut(s) 145, 197, 1235
MflI RGATCY 1 cut(s) 1165
MhlI GDGCHC 4 cut(s) 111, 447, 870, 938
MlsI TGGCCA 2 cut(s) 213, 803
MluCI AATT 2 cut(s) 656, 920
MluNI TGGCCA 2 cut(s) 213, 803
MmeI TCCRAC 1 cut(s) 486
Mox20I TGGCCA 2 cut(s) 213, 803
MscI TGGCCA 2 cut(s) 213, 803
MslI CAYNNNNRTG 2 cut(s) 489, 1116
Msp20I TGGCCA 2 cut(s) 213, 803
MspA1I CMGCKG 2 cut(s) 728, 887
MspR9I CCNGG 3 cut(s) 251, 987, 1178
MvaI CCWGG 3 cut(s) 251, 987, 1178
MwoI GCNNNNNNNGC 5 cut(s) 734, 816, 890, 1100, 1218
NcoI CCATGG 1 cut(s) 820
NdeI CATATG 1 cut(s) 530
NdeII GATC 5 cut(s) 765, 811, 994, 1026, 1165
NlaIII CATG 9 cut(s) 80, 700, 746, 799, 824, 835, 850, 1027, 1090
NlaIV GGNNCC 6 cut(s) 108, 299, 346, 634, 867, 935
NmuCI GTSAC 2 cut(s) 8, 649
NspI RCATGY 1 cut(s) 80
NspV TTCGAA 1 cut(s) 262
PagI TCATGA 1 cut(s) 742
PfeI GAWTC 3 cut(s) 399, 687, 945
PkrI GCNGC 4 cut(s) 193, 750, 886, 1174
Psp6I CCWGG 3 cut(s) 249, 985, 1176
PspFI CCCAGC 1 cut(s) 68
PspGI CCWGG 3 cut(s) 249, 985, 1176
PspN4I GGNNCC 6 cut(s) 108, 299, 346, 634, 867, 935
PspPI GGNCC 3 cut(s) 253, 1101, 1156
PsuI RGATCY 1 cut(s) 1165
PvuII CAGCTG 2 cut(s) 728, 887
RsaI GTAC 2 cut(s) 851, 913
RsaNI GTAC 2 cut(s) 850, 912
RseI CAYNNNNRTG 2 cut(s) 489, 1116
SatI GCNGC 4 cut(s) 192, 749, 885, 1173
Sau3AI GATC 5 cut(s) 765, 811, 994, 1026, 1165
Sau96I GGNCC 3 cut(s) 253, 1101, 1156
ScrFI CCNGG 3 cut(s) 251, 987, 1178
SduI GDGCHC 4 cut(s) 111, 447, 870, 938
SfaNI GCATC 2 cut(s) 25, 623
SfuI TTCGAA 1 cut(s) 262
SinI GGWCC 1 cut(s) 253
SmiMI CAYNNNNRTG 2 cut(s) 489, 1116
Sse9I AATT 2 cut(s) 656, 920
SsiI CCGC 2 cut(s) 145, 580
StyD4I CCNGG 3 cut(s) 249, 985, 1176
StyI CCWWGG 4 cut(s) 292, 820, 870, 1002
TaaI ACNGT 7 cut(s) 19, 115, 257, 406, 455, 979, 1229
TaiI ACGT 1 cut(s) 364
TaqI TCGA 1 cut(s) 262
TasI AATT 2 cut(s) 656, 920
TatI WGTACW 1 cut(s) 849
TfiI GAWTC 3 cut(s) 399, 687, 945
TseFI GTSAC 2 cut(s) 8, 649
TseI GCWGC 4 cut(s) 191, 748, 884, 1172
Tsp45I GTSAC 2 cut(s) 8, 649
TspDTI ATGAA 3 cut(s) 138, 685, 693
VpaK11BI GGWCC 1 cut(s) 253
XagI CCTNNNNNAGG 1 cut(s) 1181
XapI RAATTY 1 cut(s) 656
XceI RCATGY 1 cut(s) 80
XcmI CCANNNNNNNNNTGG 1 cut(s) 221
XmiI GTMKAC 1 cut(s) 408
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.