Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB1I | GGYRCC | 2 cut(s) | 1038, 1307 |
| AccII | CGCG | 1 cut(s) | 192 |
| AciI | CCGC | 8 cut(s) | 250, 361, 685, 833, 922, 990, 1059, 1317 |
| AclWI | GGATC | 5 cut(s) | 13, 787, 800, 911, 1278 |
| AcoI | YGGCCR | 1 cut(s) | 100 |
| AcsI | RAATTY | 1 cut(s) | 761 |
| AcuI | CTGAAG | 2 cut(s) | 142, 1341 |
| AfaI | GTAC | 6 cut(s) | 388, 845, 956, 972, 1018, 1349 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 460, 926, 1108, 1288 |
| AgsI | TTSAA | 4 cut(s) | 148, 398, 415, 1054 |
| AjiI | CACGTC | 1 cut(s) | 754 |
| AjnI | CCWGG | 3 cut(s) | 42, 582, 788 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 1235, 1267 |
| AluBI | AGCT | 4 cut(s) | 67, 173, 256, 499 |
| AluI | AGCT | 4 cut(s) | 67, 173, 256, 499 |
| Alw21I | GWGCWC | 3 cut(s) | 609, 742, 1218 |
| Alw26I | GTCTC | 2 cut(s) | 473, 699 |
| Alw44I | GTGCAC | 2 cut(s) | 738, 1214 |
| AlwI | GGATC | 5 cut(s) | 13, 787, 800, 911, 1278 |
| Ama87I | CYCGRG | 1 cut(s) | 1090 |
| AoxI | GGCC | 3 cut(s) | 100, 939, 1119 |
| ApaLI | GTGCAC | 2 cut(s) | 738, 1214 |
| ApeKI | GCWGC | 2 cut(s) | 296, 1277 |
| ApoI | RAATTY | 1 cut(s) | 761 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 1166, 1198 |
| AspLEI | GCGC | 2 cut(s) | 194, 645 |
| AspS9I | GGNCC | 3 cut(s) | 450, 778, 1119 |
| AsuC2I | CCSGG | 2 cut(s) | 1091, 1092 |
| AsuHPI | GGTGA | 4 cut(s) | 146, 865, 974, 1241 |
| AvaI | CYCGRG | 1 cut(s) | 1090 |
| AvaII | GGWCC | 2 cut(s) | 450, 778 |
| BaeGI | GKGCMC | 3 cut(s) | 742, 1043, 1218 |
| BalI | TGGCCA | 1 cut(s) | 102 |
| BamHI | GGATCC | 1 cut(s) | 792 |
| BanI | GGYRCC | 2 cut(s) | 1038, 1307 |
| BanII | GRGCYC | 3 cut(s) | 216, 552, 894 |
| BbsI | GAAGAC | 1 cut(s) | 1347 |
| Bbv12I | GWGCWC | 3 cut(s) | 609, 742, 1218 |
| BbvCI | CCTCAGC | 1 cut(s) | 1155 |
| BbvI | GCAGC | 2 cut(s) | 283, 1264 |
| BccI | CCATC | 6 cut(s) | 92, 526, 728, 898, 1010, 1328 |
| BceAI | ACGGC | 2 cut(s) | 943, 1106 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 170, 204 |
| BciT130I | CCWGG | 3 cut(s) | 44, 584, 790 |
| BciVI | GTATCC | 2 cut(s) | 50, 525 |
| BcnI | CCSGG | 2 cut(s) | 1091, 1092 |
| BcoDI | GTCTC | 2 cut(s) | 473, 699 |
| BfaI | CTAG | 2 cut(s) | 111, 1167 |
| BfmI | CTRYAG | 1 cut(s) | 637 |
| BfoI | RGCGCY | 1 cut(s) | 646 |
| BfuI | GTATCC | 2 cut(s) | 50, 525 |
| BisI | GCNGC | 4 cut(s) | 297, 990, 1060, 1278 |
| BlsI | GCNGC | 4 cut(s) | 298, 991, 1061, 1279 |
| BmcAI | AGTACT | 1 cut(s) | 1349 |
| Bme1390I | CCNGG | 5 cut(s) | 44, 584, 790, 1091, 1092 |
| Bme18I | GGWCC | 2 cut(s) | 450, 778 |
| BmeT110I | CYCGRG | 1 cut(s) | 1090 |
| BmgBI | CACGTC | 1 cut(s) | 754 |
| BmgT120I | GGNCC | 3 cut(s) | 450, 778, 1119 |
| BmiI | GGNNCC | 8 cut(s) | 404, 433, 451, 452, 780, 794, 1040, 1309 |
| BmrFI | CCNGG | 5 cut(s) | 44, 584, 790, 1091, 1092 |
| BmrI | ACTGGG | 1 cut(s) | 399 |
| BmsI | GCATC | 4 cut(s) | 130, 283, 372, 1071 |
| BmuI | ACTGGG | 1 cut(s) | 399 |
| BpiI | GAAGAC | 1 cut(s) | 1347 |
| BplI | GAGNNNNNCTC | 4 cut(s) | 455, 487, 1148, 1180 |
| BpmI | CTGGAG | 1 cut(s) | 189 |
| Bpu10I | CCTNAGC | 2 cut(s) | 267, 1155 |
| BpuEI | CTTGAG | 3 cut(s) | 98, 230, 1304 |
| BpuMI | CCSGG | 2 cut(s) | 1091, 1092 |
| BsaBI | GATNNNNATC | 1 cut(s) | 23 |
| BsaJI | CCNNGG | 7 cut(s) | 313, 788, 925, 958, 975, 1090, 1107 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 827, 857 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 460, 926, 1108, 1288 |
| Bse118I | RCCGGY | 1 cut(s) | 434 |
| Bse1I | ACTGG | 8 cut(s) | 172, 244, 394, 821, 1040, 1051, 1146, 1223 |
| Bse3DI | GCAATG | 1 cut(s) | 58 |
| Bse8I | GATNNNNATC | 1 cut(s) | 23 |
| BseBI | CCWGG | 3 cut(s) | 44, 584, 790 |
| BseDI | CCNNGG | 7 cut(s) | 313, 788, 925, 958, 975, 1090, 1107 |
| BseGI | GGATG | 4 cut(s) | 363, 896, 985, 1336 |
| BseJI | GATNNNNATC | 1 cut(s) | 23 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 460, 926, 1108, 1288 |
| BseMI | GCAATG | 1 cut(s) | 58 |
| BseMII | CTCAG | 4 cut(s) | 258, 710, 716, 1146 |
| BseNI | ACTGG | 8 cut(s) | 172, 244, 394, 821, 1040, 1051, 1146, 1223 |
| BseSI | GKGCMC | 3 cut(s) | 742, 1043, 1218 |
| BseXI | GCAGC | 2 cut(s) | 283, 1264 |
| BsgI | GTGCAG | 1 cut(s) | 1133 |
| Bsh1236I | CGCG | 1 cut(s) | 192 |
| BshFI | GGCC | 3 cut(s) | 102, 941, 1121 |
| BshNI | GGYRCC | 2 cut(s) | 1038, 1307 |
| BsiHKAI | GWGCWC | 3 cut(s) | 609, 742, 1218 |
| BsiHKCI | CYCGRG | 1 cut(s) | 1090 |
| BsiSI | CCGG | 2 cut(s) | 435, 1091 |
| BslFI | GGGAC | 3 cut(s) | 436, 764, 1073 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 460, 926, 1108, 1288 |
| BsmAI | GTCTC | 2 cut(s) | 473, 699 |
| BsmBI | CGTCTC | 1 cut(s) | 473 |
| BsmFI | GGGAC | 3 cut(s) | 436, 764, 1073 |
| BsmI | GAATGC | 2 cut(s) | 87, 991 |
| BsnI | GGCC | 3 cut(s) | 102, 941, 1121 |
| BsoBI | CYCGRG | 1 cut(s) | 1090 |
| Bsp1286I | GDGCHC | 7 cut(s) | 216, 552, 609, 742, 894, 1043, 1218 |
| Bsp143I | GATC | 7 cut(s) | 18, 205, 792, 916, 1131, 1186, 1270 |
| BspACI | CCGC | 8 cut(s) | 250, 361, 685, 833, 922, 990, 1059, 1317 |
| BspANI | GGCC | 3 cut(s) | 102, 941, 1121 |
| BspCNI | CTCAG | 4 cut(s) | 259, 709, 717, 1147 |
| BspFNI | CGCG | 1 cut(s) | 192 |
| BspLI | GGNNCC | 8 cut(s) | 404, 433, 451, 452, 780, 794, 1040, 1309 |
| BspMAI | CTGCAG | 1 cut(s) | 641 |
| BspPI | GGATC | 5 cut(s) | 13, 787, 800, 911, 1278 |
| BspQI | GCTCTTC | 1 cut(s) | 263 |
| BspT107I | GGYRCC | 2 cut(s) | 1038, 1307 |
| BsrDI | GCAATG | 1 cut(s) | 58 |
| BsrFI | RCCGGY | 1 cut(s) | 434 |
| BsrI | ACTGG | 8 cut(s) | 172, 244, 394, 821, 1040, 1051, 1146, 1223 |
| BssAI | RCCGGY | 1 cut(s) | 434 |
| BssECI | CCNNGG | 7 cut(s) | 313, 788, 925, 958, 975, 1090, 1107 |
| BssMI | GATC | 7 cut(s) | 18, 205, 792, 916, 1131, 1186, 1270 |
| BssT1I | CCWWGG | 2 cut(s) | 975, 1107 |
| Bst2UI | CCWGG | 3 cut(s) | 44, 584, 790 |
| Bst4CI | ACNGT | 8 cut(s) | 74, 220, 386, 511, 560, 881, 959, 1084 |
| Bst6I | CTCTTC | 1 cut(s) | 263 |
| BstC8I | GCNNGC | 6 cut(s) | 175, 179, 254, 641, 943, 1206 |
| BstDEI | CTNAG | 5 cut(s) | 267, 355, 696, 725, 1155 |
| BstDSI | CCRYGG | 3 cut(s) | 313, 925, 958 |
| BstENI | CCTNNNNNAGG | 2 cut(s) | 1106, 1286 |
| BstF5I | GGATG | 4 cut(s) | 363, 896, 985, 1336 |
| BstFNI | CGCG | 1 cut(s) | 192 |
| BstH2I | RGCGCY | 1 cut(s) | 646 |
| BstHHI | GCGC | 2 cut(s) | 194, 645 |
| BstKTI | GATC | 7 cut(s) | 21, 208, 795, 919, 1134, 1189, 1273 |
| BstMAI | GTCTC | 2 cut(s) | 473, 699 |
| BstMBI | GATC | 7 cut(s) | 18, 205, 792, 916, 1131, 1186, 1270 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 360, 839, 995, 1205, 1316 |
| BstNI | CCWGG | 3 cut(s) | 44, 584, 790 |
| BstNSI | RCATGY | 1 cut(s) | 185 |
| BstSCI | CCNGG | 5 cut(s) | 42, 582, 788, 1089, 1090 |
| BstSFI | CTRYAG | 1 cut(s) | 637 |
| BstSLI | GKGCMC | 3 cut(s) | 742, 1043, 1218 |
| BstUI | CGCG | 1 cut(s) | 192 |
| BstV1I | GCAGC | 2 cut(s) | 283, 1264 |
| BstV2I | GAAGAC | 1 cut(s) | 1347 |
| BstX2I | RGATCY | 2 cut(s) | 792, 1270 |
| BstYI | RGATCY | 2 cut(s) | 792, 1270 |
| BsuI | GTATCC | 2 cut(s) | 50, 525 |
| BsuRI | GGCC | 3 cut(s) | 102, 941, 1121 |
| BtgI | CCRYGG | 3 cut(s) | 313, 925, 958 |
| BtrI | CACGTC | 1 cut(s) | 754 |
| BtsCI | GGATG | 4 cut(s) | 363, 896, 985, 1336 |
| BtsI | GCAGTG | 1 cut(s) | 609 |
| BtsIMutI | CAGTG | 8 cut(s) | 166, 216, 507, 565, 609, 886, 1153, 1216 |
| Cac8I | GCNNGC | 6 cut(s) | 175, 179, 254, 641, 943, 1206 |
| CfoI | GCGC | 2 cut(s) | 194, 645 |
| Cfr10I | RCCGGY | 1 cut(s) | 434 |
| Cfr13I | GGNCC | 3 cut(s) | 450, 778, 1119 |
| Cfr9I | CCCGGG | 1 cut(s) | 1090 |
| CseI | GACGC | 1 cut(s) | 455 |
| Csp6I | GTAC | 6 cut(s) | 387, 844, 955, 971, 1017, 1348 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 205, 240 |
| CviAII | CATG | 7 cut(s) | 83, 182, 848, 901, 937, 1129, 1192 |
| CviQI | GTAC | 6 cut(s) | 387, 844, 955, 971, 1017, 1348 |
| DdeI | CTNAG | 5 cut(s) | 267, 355, 696, 725, 1155 |
| DpnI | GATC | 7 cut(s) | 20, 207, 794, 918, 1133, 1188, 1272 |
| DpnII | GATC | 7 cut(s) | 18, 205, 792, 916, 1131, 1186, 1270 |
| EaeI | YGGCCR | 1 cut(s) | 100 |
| Eam1104I | CTCTTC | 1 cut(s) | 263 |
| EarI | CTCTTC | 1 cut(s) | 263 |
| Eco130I | CCWWGG | 2 cut(s) | 975, 1107 |
| Eco24I | GRGCYC | 3 cut(s) | 216, 552, 894 |
| Eco32I | GATATC | 1 cut(s) | 634 |
| Eco47I | GGWCC | 2 cut(s) | 450, 778 |
| Eco57I | CTGAAG | 2 cut(s) | 142, 1341 |
| Eco88I | CYCGRG | 1 cut(s) | 1090 |
| EcoNI | CCTNNNNNAGG | 2 cut(s) | 1106, 1286 |
| EcoO109I | RGGNCCY | 1 cut(s) | 450 |
| EcoRI | GAATTC | 1 cut(s) | 761 |
| EcoRII | CCWGG | 3 cut(s) | 42, 582, 788 |
| EcoRV | GATATC | 1 cut(s) | 634 |
| EcoT14I | CCWWGG | 2 cut(s) | 975, 1107 |
| EcoT38I | GRGCYC | 3 cut(s) | 216, 552, 894 |
| ErhI | CCWWGG | 2 cut(s) | 975, 1107 |
| Esp3I | CGTCTC | 1 cut(s) | 473 |
| FaeI | CATG | 7 cut(s) | 86, 185, 851, 904, 940, 1132, 1195 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 390, 422, 1193, 1225 |
| FaqI | GGGAC | 3 cut(s) | 436, 764, 1073 |
| FatI | CATG | 7 cut(s) | 82, 181, 847, 900, 936, 1128, 1191 |
| FauI | CCCGC | 1 cut(s) | 929 |
| FauNDI | CATATG | 1 cut(s) | 814 |
| Fnu4HI | GCNGC | 4 cut(s) | 297, 990, 1060, 1278 |
| FokI | GGATG | 4 cut(s) | 350, 883, 992, 1343 |
| FriOI | GRGCYC | 3 cut(s) | 216, 552, 894 |
| Fsp4HI | GCNGC | 4 cut(s) | 297, 990, 1060, 1278 |
| FspBI | CTAG | 2 cut(s) | 111, 1167 |
| GlaI | GCGC | 2 cut(s) | 193, 644 |
| GluI | GCNGC | 4 cut(s) | 297, 990, 1060, 1278 |
| GsuI | CTGGAG | 1 cut(s) | 189 |
| HaeII | RGCGCY | 1 cut(s) | 646 |
| HaeIII | GGCC | 3 cut(s) | 102, 941, 1121 |
| HapII | CCGG | 2 cut(s) | 435, 1091 |
| HgaI | GACGC | 1 cut(s) | 455 |
| HhaI | GCGC | 2 cut(s) | 194, 645 |
| Hin1II | CATG | 7 cut(s) | 86, 185, 851, 904, 940, 1132, 1195 |
| Hin6I | GCGC | 2 cut(s) | 192, 643 |
| HinP1I | GCGC | 2 cut(s) | 192, 643 |
| HincII | GTYRAC | 2 cut(s) | 346, 478 |
| HindII | GTYRAC | 2 cut(s) | 346, 478 |
| HindIII | AAGCTT | 1 cut(s) | 497 |
| HinfI | GANTC | 2 cut(s) | 1050, 1313 |
| HpaII | CCGG | 2 cut(s) | 435, 1091 |
| HphI | GGTGA | 4 cut(s) | 146, 865, 974, 1241 |
| Hpy166II | GTNNAC | 7 cut(s) | 346, 371, 478, 740, 751, 1033, 1216 |
| Hpy188I | TCNGA | 3 cut(s) | 726, 951, 1258 |
| Hpy188III | TCNNGA | 6 cut(s) | 77, 209, 650, 766, 1006, 1167 |
| Hpy8I | GTNNAC | 7 cut(s) | 346, 371, 478, 740, 751, 1033, 1216 |
| HpyAV | CCTTC | 7 cut(s) | 154, 163, 464, 832, 1238, 1282, 1316 |
| HpyCH4III | ACNGT | 8 cut(s) | 74, 220, 386, 511, 560, 881, 959, 1084 |
| HpyCH4IV | ACGT | 2 cut(s) | 753, 966 |
| HpyCH4V | TGCA | 8 cut(s) | 12, 602, 639, 740, 818, 1114, 1128, 1216 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 360, 839, 995, 1205, 1316 |
| HpyF3I | CTNAG | 5 cut(s) | 267, 355, 696, 725, 1155 |
| HpySE526I | ACGT | 2 cut(s) | 753, 966 |
| Hsp92II | CATG | 7 cut(s) | 86, 185, 851, 904, 940, 1132, 1195 |
| HspAI | GCGC | 2 cut(s) | 192, 643 |
| KflI | GGGWCCC | 1 cut(s) | 450 |
| Kzo9I | GATC | 7 cut(s) | 18, 205, 792, 916, 1131, 1186, 1270 |
| LguI | GCTCTTC | 1 cut(s) | 263 |
| LmnI | GCTCC | 4 cut(s) | 64, 170, 463, 612 |
| Lsp1109I | GCAGC | 2 cut(s) | 283, 1264 |
| LweI | GCATC | 4 cut(s) | 130, 283, 372, 1071 |
| MaeI | CTAG | 2 cut(s) | 111, 1167 |
| MaeII | ACGT | 2 cut(s) | 753, 966 |
| MaeIII | GTNAC | 3 cut(s) | 113, 754, 962 |
| MalI | GATC | 7 cut(s) | 20, 207, 794, 918, 1133, 1188, 1272 |
| MboI | GATC | 7 cut(s) | 18, 205, 792, 916, 1131, 1186, 1270 |
| MboII | GAAGA | 5 cut(s) | 148, 250, 302, 1310, 1352 |
| MflI | RGATCY | 2 cut(s) | 792, 1270 |
| MhlI | GDGCHC | 7 cut(s) | 216, 552, 609, 742, 894, 1043, 1218 |
| MlsI | TGGCCA | 1 cut(s) | 102 |
| MluCI | AATT | 2 cut(s) | 761, 1170 |
| MluNI | TGGCCA | 1 cut(s) | 102 |
| MlyI | GAGTC | 1 cut(s) | 1322 |
| MmeI | TCCRAC | 1 cut(s) | 1161 |
| Mox20I | TGGCCA | 1 cut(s) | 102 |
| MscI | TGGCCA | 1 cut(s) | 102 |
| MseI | TTAA | 1 cut(s) | 744 |
| MslI | CAYNNNNRTG | 2 cut(s) | 594, 1221 |
| Msp20I | TGGCCA | 1 cut(s) | 102 |
| MspA1I | CMGCKG | 2 cut(s) | 992, 1319 |
| MspI | CCGG | 2 cut(s) | 435, 1091 |
| MspR9I | CCNGG | 5 cut(s) | 44, 584, 790, 1091, 1092 |
| Mva1269I | GAATGC | 2 cut(s) | 87, 991 |
| MvaI | CCWGG | 3 cut(s) | 44, 584, 790 |
| MvnI | CGCG | 1 cut(s) | 192 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 360, 839, 995, 1205, 1316 |
| NciI | CCSGG | 2 cut(s) | 1091, 1092 |
| NdeI | CATATG | 1 cut(s) | 814 |
| NdeII | GATC | 7 cut(s) | 18, 205, 792, 916, 1131, 1186, 1270 |
| NlaIII | CATG | 7 cut(s) | 86, 185, 851, 904, 940, 1132, 1195 |
| NlaIV | GGNNCC | 8 cut(s) | 404, 433, 451, 452, 780, 794, 1040, 1309 |
| NmeAIII | GCCGAG | 1 cut(s) | 1336 |
| NmuCI | GTSAC | 3 cut(s) | 113, 754, 962 |
| NspI | RCATGY | 1 cut(s) | 185 |
| PciSI | GCTCTTC | 1 cut(s) | 263 |
| PctI | GAATGC | 2 cut(s) | 87, 991 |
| PfeI | GAWTC | 1 cut(s) | 1050 |
| PfoI | TCCNGGA | 1 cut(s) | 582 |
| PkrI | GCNGC | 4 cut(s) | 298, 991, 1061, 1279 |
| PleI | GAGTC | 1 cut(s) | 1321 |
| PpsI | GAGTC | 1 cut(s) | 1321 |
| PpuMI | RGGWCCY | 1 cut(s) | 450 |
| Psp5II | RGGWCCY | 1 cut(s) | 450 |
| Psp6I | CCWGG | 3 cut(s) | 42, 582, 788 |
| PspGI | CCWGG | 3 cut(s) | 42, 582, 788 |
| PspN4I | GGNNCC | 8 cut(s) | 404, 433, 451, 452, 780, 794, 1040, 1309 |
| PspPI | GGNCC | 3 cut(s) | 450, 778, 1119 |
| PspPPI | RGGWCCY | 1 cut(s) | 450 |
| PstI | CTGCAG | 1 cut(s) | 641 |
| PsuI | RGATCY | 2 cut(s) | 792, 1270 |
| RsaI | GTAC | 6 cut(s) | 388, 845, 956, 972, 1018, 1349 |
| RsaNI | GTAC | 6 cut(s) | 387, 844, 955, 971, 1017, 1348 |
| RseI | CAYNNNNRTG | 2 cut(s) | 594, 1221 |
| SapI | GCTCTTC | 1 cut(s) | 263 |
| SaqAI | TTAA | 1 cut(s) | 744 |
| SatI | GCNGC | 4 cut(s) | 297, 990, 1060, 1278 |
| Sau3AI | GATC | 7 cut(s) | 18, 205, 792, 916, 1131, 1186, 1270 |
| Sau96I | GGNCC | 3 cut(s) | 450, 778, 1119 |
| ScaI | AGTACT | 1 cut(s) | 1349 |
| SchI | GAGTC | 1 cut(s) | 1322 |
| ScrFI | CCNGG | 5 cut(s) | 44, 584, 790, 1091, 1092 |
| SduI | GDGCHC | 7 cut(s) | 216, 552, 609, 742, 894, 1043, 1218 |
| SfaNI | GCATC | 4 cut(s) | 130, 283, 372, 1071 |
| SfcI | CTRYAG | 1 cut(s) | 637 |
| SinI | GGWCC | 2 cut(s) | 450, 778 |
| SmaI | CCCGGG | 1 cut(s) | 1092 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 594, 1221 |
| SmlI | CTYRAG | 3 cut(s) | 77, 209, 1283 |
| SmoI | CTYRAG | 3 cut(s) | 77, 209, 1283 |
| Sse9I | AATT | 2 cut(s) | 761, 1170 |
| SsiI | CCGC | 8 cut(s) | 250, 361, 685, 833, 922, 990, 1059, 1317 |
| SspMI | CTAG | 2 cut(s) | 111, 1167 |
| StyD4I | CCNGG | 5 cut(s) | 42, 582, 788, 1089, 1090 |
| StyI | CCWWGG | 2 cut(s) | 975, 1107 |
| TaaI | ACNGT | 8 cut(s) | 74, 220, 386, 511, 560, 881, 959, 1084 |
| TaiI | ACGT | 2 cut(s) | 756, 969 |
| TaqI | TCGA | 3 cut(s) | 17, 732, 1142 |
| TaqII | GACCGA | 1 cut(s) | 17 |
| TasI | AATT | 2 cut(s) | 761, 1170 |
| TatI | WGTACW | 2 cut(s) | 386, 1347 |
| TauI | GCSGC | 2 cut(s) | 992, 1062 |
| TfiI | GAWTC | 1 cut(s) | 1050 |
| Tru1I | TTAA | 1 cut(s) | 744 |
| Tru9I | TTAA | 1 cut(s) | 744 |
| TscAI | CASTG | 8 cut(s) | 166, 223, 514, 565, 609, 886, 1153, 1223 |
| TseFI | GTSAC | 3 cut(s) | 113, 754, 962 |
| TseI | GCWGC | 2 cut(s) | 296, 1277 |
| Tsp45I | GTSAC | 3 cut(s) | 113, 754, 962 |
| TspDTI | ATGAA | 3 cut(s) | 243, 864, 1311 |
| TspGWI | ACGGA | 1 cut(s) | 356 |
| TspMI | CCCGGG | 1 cut(s) | 1090 |
| TspRI | CASTG | 8 cut(s) | 166, 223, 514, 565, 609, 886, 1153, 1223 |
| VneI | GTGCAC | 2 cut(s) | 738, 1214 |
| VpaK11BI | GGWCC | 2 cut(s) | 450, 778 |
| XagI | CCTNNNNNAGG | 2 cut(s) | 1106, 1286 |
| XapI | RAATTY | 1 cut(s) | 761 |
| XbaI | TCTAGA | 1 cut(s) | 1166 |
| XceI | RCATGY | 1 cut(s) | 185 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 1027 |
| XmaI | CCCGGG | 1 cut(s) | 1090 |
| XspI | CTAG | 2 cut(s) | 111, 1167 |
| ZrmI | AGTACT | 1 cut(s) | 1349 |