FvH4_5g33560

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0000549
Physical Location & Seq
Reverse (-)
24282873 .. 24285437
2565 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g33560.t1

Sequence Viewer

Length: 1356 bp
ATGAGAGAGTGCATTTCGATCCACATCGGCCAGGCCGGAATCCAGGTCGGAAACGCCTGCTGGGAGCTCTACTGCCTCGAGCATGGGATCCAGCCGGACGGCCAGATGCCGAGCGACAAGACCGTCGGCCGCGGCGACGACGCCTTCAACACCTTCTTCTCCGAGACCGGCGCCGGCAAACACGTCCCCCGCGCCATCTTCGTCGACCTCGAGCCCACCGTCATCGACGAGGTCCGCACCGGTACCTACCGCCAGCTCTTCCACCCCGAGCAGCTCATCTCCGGCAAGGAGGACGCCGCCAACAACTTTGCCCGTGGCCACTACACCATCGGCAAAGAGATCGTCGATCTCTGCTTGGATCGGATCCGGAAGCTCGCCGATAACTGCACCGGGCTCCAGGGGTTCCTGGTGTTCAATGCCGTCGGTGGAGGTACCGGGTCGGGTCTCGGGTCGCTTCTTCTGGAGAGGCTTTCGGTGGACTATGGGAAAAAGTCCAAGCTCGGATTCACTGTGTATCCTTCTCCTCAGGTTTCCACATCTGTTGTGGAGCCGTATAACAGTGTGCTTTCTACTCATTCTCTGCTTGAGCACACCGATGTGTCTGTGCTGCTTGATAATGAGGCCATCTATGACATCTGCCGCAGATCCCTGGACATTGAGCGTCCAACTTACACCAATCTCAACCGCCTCGTCTCTCAGGTGATTTCATCTTTGACTGCCTCTTTGAGGTTCGATGGGGCTCTAAATGTGGATGTGACTGAGTTCCAGACCAACTTGGTGCCATACCCGAGGATTCATTTCATGCTTTCCTCCTATGCTCCTGTGATCTCCGCAGAGAAAGCATACCATGAGCAGCTCTCAGTGGCTGAGATCACCAACAGTGCTTTTGAGCCTGCTTCCATGATGGCCAAGTGTGATCCTCGCCATGGAAAGTACATGGCCTGCTGTTTGATGTACCGAGGAGATGTGGTGCCAAAGGATGTGAATGCGGCTGTGGCGACCATCAAGACCAAGCGTACCATTCAGTTTGTGGACTGGTGCCCTACTGGGTTCAAGTGTGGTATCAACTACCAGCCACCCACTGTTGTTCCAGGTGGTGACCTTGCTAAGGTGCAGAGGGCTGTGTGCATGATCTCCAACTCCACCAGTGTGGCTGAGGTGTTCTCTCGTATCGATCACAAGTTTGATTTGATGTATGCCAAGCGTGCTTTTGTGCATTGGTATGTGGGTGAGGGCATGGAGGAAGGAGAGTTCTCTGAGGCCCGTGAGGATCTTGCTGCTCTGGAGAAGGATTATGAGGAGGTTGGCGCCGAGTCTGCTGAGGGAGATGATGGCGATGAAGGTGACGAGTACTGA

Protein Analysis

452

Amino Acids

49.73

Weight (kDa)

4.96

Isoelectric Point (pI)

35.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tubulin PF00091 3 - 213 8.3e-63 Tubulin/FtsZ family, GTPase domain
Tubulin_C PF03953 263 - 392 8e-52 Tubulin C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000549)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G04820 AT1G50010 AT4G14960 AT4G14960
fragaria_vesca FvH4_1g18660 FvH4_2g03140 FvH4_5g33560 FvH4_6g48940 FvH4_6g48940
malus_domestica MD02G1204800.v1.1 MD05G1106800.v1.1 MD08G1210600.v1.1 MD09G1049500.v1.1 MD10G1111600.v1.1 MD15G1398700.v1.1 MD17G1049700.v1.1
prunus_persica Prupe.1G542600_v2.0.a1 Prupe.3G269300_v2.0.a1 Prupe.6G212700_v2.0.a1 Prupe.8G150900_v2.0.a1
pyrus_communis pycom05g10370 pycom08g18150 pycom10g09640 pycom10g09660 pycom111g04050 pycom15g35470 pycom17g04460
rosa_chinensis RchiOBHm_Chr2g0109481 RchiOBHm_Chr2g0168751 RchiOBHm_Chr3g0453071 RchiOBHm_Chr3g0460741 RchiOBHm_Chr3g0467971 RchiOBHm_Chr4g0400901 RchiOBHm_Chr6g0248011 RchiOBHm_Chr7g0232381 RchiOBHm_Chr7g0238121
rosa_laevigata RLG00000001350 RLG00000009167 RLG00000015163 RLG00000017708 RLG00000017741 RLG00000021807 RLG00000023849 RLG00000026518
rosa_multiflora Rmu_sc0000332.1_g000021 Rmu_sc0000332.1_g000029 Rmu_sc0002416.1_g000007 Rmu_sc0002611.1_g000001 Rmu_sc0003113.1_g000016 Rmu_sc0004780.1_g000001 Rmu_sc0004805.1_g000035 Rmu_sc0010633.1_g000010 Rmu_sc0011069.1_g000003 Rmu_sc0016170.1_g000002 Rmu_sc0025757.1_g000003 Rmu_ssc0000484.1_g000003
rosa_roxburghii Rroxscaffold_2G00134200 Rroxscaffold_4G00321030 Rroxscaffold_7G00213500
rosa_rugosa Rorug01G0283100 Rorug02G0160500 Rorug02G0535300 Rorug04G0027000 Rorug04G0328100 Rorug05G0388700 Rorug05G0530200 Rorug06G0489200 Rorug07G0104900 Rorug07G0209400 Rorug07G0274500
rosa_samantha Rh2BG222800 Rh2BG618900 Rh2CG214400 Rh2CG587200 Rh6BG040200 Rh6CG206500 Rh6DG035600 Rh7BG401600 Rh7BG401700 Rh7DG418400
rosa_wichuraiana Rw2G050240 Rw6G003920 Rw7G035430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 122
Acc65I GGTACC 2 cut(s) 242, 431
AccB1I GGYRCC 7 cut(s) 170, 242, 431, 778, 970, 1038, 1307
AccI GTMKAC 1 cut(s) 204
AccII CGCG 2 cut(s) 132, 192
AccIII TCCGGA 1 cut(s) 366
AclWI GGATC 9 cut(s) 13, 82, 95, 358, 366, 371, 639, 911, 1278
AcoI YGGCCR 5 cut(s) 28, 100, 127, 316, 906
AcyI GRCGYC 4 cut(s) 141, 171, 294, 1308
AfaI GTAC 6 cut(s) 244, 433, 935, 956, 1018, 1352
AfiI CCNNNNNNNGG 3 cut(s) 726, 926, 1108
AflIII ACRYGT 1 cut(s) 181
AgeI ACCGGT 1 cut(s) 239
AgsI TTSAA 3 cut(s) 148, 415, 1054
AjiI CACGTC 1 cut(s) 184
AjnI CCWGG 6 cut(s) 30, 42, 396, 405, 648, 1090
AjuI GAANNNNNNNTTGG 2 cut(s) 488, 520
AleI CACNNNNGTG 2 cut(s) 596, 1148
AloI GAACNNNNNNTCC 2 cut(s) 1235, 1267
AluBI AGCT 6 cut(s) 67, 256, 274, 373, 499, 856
AluI AGCT 6 cut(s) 67, 256, 274, 373, 499, 856
Alw21I GWGCWC 2 cut(s) 69, 591
Alw26I GTCTC 3 cut(s) 158, 449, 697
AlwI GGATC 9 cut(s) 13, 82, 95, 358, 366, 371, 639, 911, 1278
AlwNI CAGNNNCTG 1 cut(s) 866
Ama87I CYCGRG 5 cut(s) 77, 209, 266, 446, 787
Aor13HI TCCGGA 1 cut(s) 366
AoxI GGCC 9 cut(s) 28, 33, 100, 127, 316, 621, 906, 939, 1260
ApeKI GCWGC 4 cut(s) 271, 607, 853, 1277
ArsI GACNNNNNNTTYG 4 cut(s) 327, 359, 706, 738
AsiGI ACCGGT 1 cut(s) 239
Asp718I GGTACC 2 cut(s) 242, 431
AspLEI GCGC 3 cut(s) 173, 194, 1310
AspS9I GGNCC 2 cut(s) 232, 1261
AsuC2I CCSGG 2 cut(s) 391, 436
AsuHPI GGTGA 5 cut(s) 712, 865, 1109, 1241, 1355
AvaI CYCGRG 5 cut(s) 77, 209, 266, 446, 787
AvaII GGWCC 1 cut(s) 232
AxyI CCTNAGG 1 cut(s) 525
BaeGI GKGCMC 1 cut(s) 1043
BalI TGGCCA 2 cut(s) 318, 908
BamHI GGATCC 2 cut(s) 87, 363
BanI GGYRCC 7 cut(s) 170, 242, 431, 778, 970, 1038, 1307
BanII GRGCYC 4 cut(s) 69, 216, 396, 742
Bbv12I GWGCWC 2 cut(s) 69, 591
BbvCI CCTCAGC 2 cut(s) 1155, 1320
BbvI GCAGC 4 cut(s) 283, 594, 865, 1264
BccI CCATC 7 cut(s) 203, 335, 632, 728, 898, 1010, 1325
BceAI ACGGC 3 cut(s) 115, 404, 535
BcgI CGANNNNNNTGC 2 cut(s) 322, 356
BciT130I CCWGG 6 cut(s) 32, 44, 398, 407, 650, 1092
BciVI GTATCC 1 cut(s) 525
BcnI CCSGG 2 cut(s) 391, 436
BcoDI GTCTC 3 cut(s) 158, 449, 697
BfoI RGCGCY 2 cut(s) 174, 1311
BfuI GTATCC 1 cut(s) 525
BisI GCNGC 9 cut(s) 130, 133, 272, 297, 608, 640, 854, 990, 1278
BlsI GCNGC 9 cut(s) 131, 134, 273, 298, 609, 641, 855, 991, 1279
BmcAI AGTACT 1 cut(s) 1352
Bme1390I CCNGG 8 cut(s) 32, 44, 391, 398, 407, 436, 650, 1092
Bme18I GGWCC 1 cut(s) 232
BmeT110I CYCGRG 5 cut(s) 77, 209, 266, 446, 787
BmgBI CACGTC 1 cut(s) 184
BmgT120I GGNCC 2 cut(s) 232, 1261
BmrFI CCNGG 8 cut(s) 32, 44, 391, 398, 407, 436, 650, 1092
BmrI ACTGGG 1 cut(s) 1056
BmsI GCATC 1 cut(s) 96
BmuI ACTGGG 1 cut(s) 1056
BplI GAGNNNNNCTC 4 cut(s) 842, 874, 1148, 1180
BpmI CTGGAG 3 cut(s) 380, 482, 1304
Bpu10I CCTNAGC 3 cut(s) 1107, 1155, 1320
BpuEI CTTGAG 1 cut(s) 605
BpuMI CCSGG 2 cut(s) 391, 436
Bsa29I ATCGAT 1 cut(s) 1173
BsaBI GATNNNNATC 1 cut(s) 23
BsaHI GRCGYC 4 cut(s) 141, 171, 294, 1308
BsaI GGTCTC 2 cut(s) 158, 449
BsaJI CCNNGG 7 cut(s) 130, 313, 397, 648, 788, 925, 958
BsaWI WCCGGW 2 cut(s) 239, 366
BsaXI ACNNNNNCTCC 2 cut(s) 954, 984
Bsc4I CCNNNNNNNGG 3 cut(s) 726, 926, 1108
Bse118I RCCGGY 3 cut(s) 167, 173, 239
Bse1I ACTGG 3 cut(s) 1040, 1051, 1146
Bse21I CCTNAGG 1 cut(s) 525
Bse8I GATNNNNATC 1 cut(s) 23
BseAI TCCGGA 1 cut(s) 366
BseBI CCWGG 6 cut(s) 32, 44, 398, 407, 650, 1092
BseCI ATCGAT 1 cut(s) 1173
BseDI CCNNGG 7 cut(s) 130, 313, 397, 648, 788, 925, 958
BseGI GGATG 2 cut(s) 757, 985
BseJI GATNNNNATC 1 cut(s) 23
BseLI CCNNNNNNNGG 3 cut(s) 726, 926, 1108
BseMII CTCAG 8 cut(s) 539, 710, 750, 858, 873, 1146, 1248, 1311
BseNI ACTGG 3 cut(s) 1040, 1051, 1146
BseRI GAGGAG 3 cut(s) 513, 975, 1313
BseSI GKGCMC 1 cut(s) 1043
BseX3I CGGCCG 1 cut(s) 127
BseXI GCAGC 4 cut(s) 283, 594, 865, 1264
BseYI CCCAGC 1 cut(s) 60
BsgI GTGCAG 2 cut(s) 370, 1133
Bsh1236I CGCG 2 cut(s) 132, 192
Bsh1285I CGRYCG 1 cut(s) 130
BshFI GGCC 9 cut(s) 30, 35, 102, 129, 318, 623, 908, 941, 1262
BshNI GGYRCC 7 cut(s) 170, 242, 431, 778, 970, 1038, 1307
BshTI ACCGGT 1 cut(s) 239
BshVI ATCGAT 1 cut(s) 1173
BsiEI CGRYCG 1 cut(s) 130
BsiHKAI GWGCWC 2 cut(s) 69, 591
BsiHKCI CYCGRG 5 cut(s) 77, 209, 266, 446, 787
BsiSI CCGG 9 cut(s) 36, 95, 168, 174, 240, 282, 367, 390, 435
BslFI GGGAC 1 cut(s) 170
BslI CCNNNNNNNGG 3 cut(s) 726, 926, 1108
BsmAI GTCTC 3 cut(s) 158, 449, 697
BsmBI CGTCTC 1 cut(s) 697
BsmFI GGGAC 1 cut(s) 170
BsmI GAATGC 1 cut(s) 991
BsnI GGCC 9 cut(s) 30, 35, 102, 129, 318, 623, 908, 941, 1262
Bso31I GGTCTC 2 cut(s) 158, 449
BsoBI CYCGRG 5 cut(s) 77, 209, 266, 446, 787
Bsp1286I GDGCHC 6 cut(s) 69, 216, 396, 591, 742, 1043
Bsp13I TCCGGA 1 cut(s) 366
Bsp19I CCATGG 1 cut(s) 925
BspANI GGCC 9 cut(s) 30, 35, 102, 129, 318, 623, 908, 941, 1262
BspCNI CTCAG 8 cut(s) 538, 709, 751, 859, 872, 1147, 1249, 1312
BspDI ATCGAT 1 cut(s) 1173
BspEI TCCGGA 1 cut(s) 366
BspFNI CGCG 2 cut(s) 132, 192
BspPI GGATC 9 cut(s) 13, 82, 95, 358, 366, 371, 639, 911, 1278
BspQI GCTCTTC 1 cut(s) 263
BspT107I GGYRCC 7 cut(s) 170, 242, 431, 778, 970, 1038, 1307
BspTNI GGTCTC 2 cut(s) 158, 449
BsrFI RCCGGY 3 cut(s) 167, 173, 239
BsrI ACTGG 3 cut(s) 1040, 1051, 1146
BssAI RCCGGY 3 cut(s) 167, 173, 239
BssECI CCNNGG 7 cut(s) 130, 313, 397, 648, 788, 925, 958
BssNI GRCGYC 4 cut(s) 141, 171, 294, 1308
BssT1I CCWWGG 1 cut(s) 925
Bst2UI CCWGG 6 cut(s) 32, 44, 398, 407, 650, 1092
Bst4CI ACNGT 6 cut(s) 124, 220, 511, 560, 881, 1084
Bst6I CTCTTC 1 cut(s) 263
BstACI GRCGYC 4 cut(s) 141, 171, 294, 1308
BstC8I GCNNGC 7 cut(s) 58, 175, 254, 375, 894, 943, 1206
BstDEI CTNAG 9 cut(s) 525, 696, 759, 859, 867, 1107, 1155, 1257, 1320
BstDSI CCRYGG 3 cut(s) 130, 313, 925
BstEII GGTNACC 1 cut(s) 1097
BstENI CCTNNNNNAGG 2 cut(s) 724, 1106
BstF5I GGATG 2 cut(s) 757, 985
BstFNI CGCG 2 cut(s) 132, 192
BstH2I RGCGCY 2 cut(s) 174, 1311
BstHHI GCGC 3 cut(s) 173, 194, 1310
BstMAI GTCTC 3 cut(s) 158, 449, 697
BstMCI CGRYCG 1 cut(s) 130
BstMWI GCNNNNNNNGC 4 cut(s) 839, 995, 1205, 1316
BstNI CCWGG 6 cut(s) 32, 44, 398, 407, 650, 1092
BstPI GGTNACC 1 cut(s) 1097
BstSCI CCNGG 8 cut(s) 30, 42, 389, 396, 405, 434, 648, 1090
BstSLI GKGCMC 1 cut(s) 1043
BstUI CGCG 2 cut(s) 132, 192
BstV1I GCAGC 4 cut(s) 283, 594, 865, 1264
BstX2I RGATCY 4 cut(s) 87, 363, 644, 1270
BstXI CCANNNNNNTGG 1 cut(s) 1150
BstYI RGATCY 4 cut(s) 87, 363, 644, 1270
BstZI CGGCCG 1 cut(s) 127
Bsu15I ATCGAT 1 cut(s) 1173
Bsu36I CCTNAGG 1 cut(s) 525
BsuI GTATCC 1 cut(s) 525
BsuRI GGCC 9 cut(s) 30, 35, 102, 129, 318, 623, 908, 941, 1262
BsuTUI ATCGAT 1 cut(s) 1173
BtgI CCRYGG 3 cut(s) 130, 313, 925
BtgZI GCGATG 1 cut(s) 1350
BtrI CACGTC 1 cut(s) 184
BtsCI GGATG 2 cut(s) 757, 985
BtsIMutI CAGTG 6 cut(s) 507, 565, 867, 886, 1080, 1153
Cac8I GCNNGC 7 cut(s) 58, 175, 254, 375, 894, 943, 1206
CaiI CAGNNNCTG 1 cut(s) 866
CfoI GCGC 3 cut(s) 173, 194, 1310
Cfr10I RCCGGY 3 cut(s) 167, 173, 239
Cfr13I GGNCC 2 cut(s) 232, 1261
Cfr42I CCGCGG 1 cut(s) 133
ClaI ATCGAT 1 cut(s) 1173
CseI GACGC 3 cut(s) 149, 302, 650
Csp6I GTAC 6 cut(s) 243, 432, 934, 955, 1017, 1351
CspAI ACCGGT 1 cut(s) 239
CspCI CAANNNNNGTGG 2 cut(s) 523, 558
CviAII CATG 8 cut(s) 83, 802, 848, 901, 926, 937, 1129, 1237
CviQI GTAC 6 cut(s) 243, 432, 934, 955, 1017, 1351
DdeI CTNAG 9 cut(s) 525, 696, 759, 859, 867, 1107, 1155, 1257, 1320
DinI GGCGCC 2 cut(s) 172, 1309
DrdI GACNNNNNNGTC 1 cut(s) 122
DseDI GACNNNNNNGTC 1 cut(s) 122
EaeI YGGCCR 5 cut(s) 28, 100, 127, 316, 906
EagI CGGCCG 1 cut(s) 127
Eam1104I CTCTTC 1 cut(s) 263
EarI CTCTTC 1 cut(s) 263
Ecl136II GAGCTC 1 cut(s) 67
EclXI CGGCCG 1 cut(s) 127
Eco130I CCWWGG 1 cut(s) 925
Eco24I GRGCYC 4 cut(s) 69, 216, 396, 742
Eco31I GGTCTC 2 cut(s) 158, 449
Eco47I GGWCC 1 cut(s) 232
Eco52I CGGCCG 1 cut(s) 127
Eco53kI GAGCTC 1 cut(s) 67
Eco81I CCTNAGG 1 cut(s) 525
Eco88I CYCGRG 5 cut(s) 77, 209, 266, 446, 787
Eco91I GGTNACC 1 cut(s) 1097
EcoICRI GAGCTC 1 cut(s) 67
EcoNI CCTNNNNNAGG 2 cut(s) 724, 1106
EcoO65I GGTNACC 1 cut(s) 1097
EcoRII CCWGG 6 cut(s) 30, 42, 396, 405, 648, 1090
EcoT14I CCWWGG 1 cut(s) 925
EcoT38I GRGCYC 4 cut(s) 69, 216, 396, 742
EgeI GGCGCC 2 cut(s) 172, 1309
EheI GGCGCC 2 cut(s) 172, 1309
ErhI CCWWGG 1 cut(s) 925
Esp3I CGTCTC 1 cut(s) 697
FaeI CATG 8 cut(s) 86, 805, 851, 904, 929, 940, 1132, 1240
FaqI GGGAC 1 cut(s) 170
FatI CATG 8 cut(s) 82, 801, 847, 900, 925, 936, 1128, 1236
FauI CCCGC 1 cut(s) 197
FblI GTMKAC 1 cut(s) 204
Fnu4HI GCNGC 9 cut(s) 130, 133, 272, 297, 608, 640, 854, 990, 1278
FokI GGATG 2 cut(s) 764, 992
FriOI GRGCYC 4 cut(s) 69, 216, 396, 742
Fsp4HI GCNGC 9 cut(s) 130, 133, 272, 297, 608, 640, 854, 990, 1278
GlaI GCGC 3 cut(s) 172, 193, 1309
GluI GCNGC 9 cut(s) 130, 133, 272, 297, 608, 640, 854, 990, 1278
GsaI CCCAGC 1 cut(s) 64
GsuI CTGGAG 3 cut(s) 380, 482, 1304
HaeII RGCGCY 2 cut(s) 174, 1311
HaeIII GGCC 9 cut(s) 30, 35, 102, 129, 318, 623, 908, 941, 1262
HapII CCGG 9 cut(s) 36, 95, 168, 174, 240, 282, 367, 390, 435
HgaI GACGC 3 cut(s) 149, 302, 650
HhaI GCGC 3 cut(s) 173, 194, 1310
Hin1I GRCGYC 4 cut(s) 141, 171, 294, 1308
Hin1II CATG 8 cut(s) 86, 805, 851, 904, 929, 940, 1132, 1240
Hin6I GCGC 3 cut(s) 171, 192, 1308
HinP1I GCGC 3 cut(s) 171, 192, 1308
HincII GTYRAC 1 cut(s) 205
HindII GTYRAC 1 cut(s) 205
HinfI GANTC 4 cut(s) 39, 504, 793, 1313
HpaII CCGG 9 cut(s) 36, 95, 168, 174, 240, 282, 367, 390, 435
HphI GGTGA 5 cut(s) 712, 865, 1109, 1241, 1355
Hpy166II GTNNAC 3 cut(s) 205, 478, 1033
Hpy188I TCNGA 5 cut(s) 50, 163, 363, 503, 1258
Hpy188III TCNNGA 5 cut(s) 367, 461, 766, 1006, 1283
Hpy8I GTNNAC 3 cut(s) 205, 478, 1033
Hpy99I CGWCG 7 cut(s) 128, 140, 143, 206, 230, 347, 425
HpyAV CCTTC 6 cut(s) 154, 163, 528, 1238, 1282, 1334
HpyCH4III ACNGT 6 cut(s) 124, 220, 511, 560, 881, 1084
HpyCH4IV ACGT 1 cut(s) 183
HpyCH4V TGCA 5 cut(s) 12, 387, 1114, 1128, 1216
HpyF10VI GCNNNNNNNGC 4 cut(s) 839, 995, 1205, 1316
HpyF3I CTNAG 9 cut(s) 525, 696, 759, 859, 867, 1107, 1155, 1257, 1320
HpySE526I ACGT 1 cut(s) 183
Hsp92I GRCGYC 4 cut(s) 141, 171, 294, 1308
Hsp92II CATG 8 cut(s) 86, 805, 851, 904, 929, 940, 1132, 1240
HspAI GCGC 3 cut(s) 171, 192, 1308
KasI GGCGCC 2 cut(s) 170, 1307
Kpn2I TCCGGA 1 cut(s) 366
KpnI GGTACC 2 cut(s) 246, 435
KroI GCCGGC 1 cut(s) 173
KroNI GCCGGC 1 cut(s) 175
KspI CCGCGG 1 cut(s) 133
LguI GCTCTTC 1 cut(s) 263
LmnI GCTCC 4 cut(s) 64, 399, 547, 823
Lsp1109I GCAGC 4 cut(s) 283, 594, 865, 1264
LweI GCATC 1 cut(s) 96
MaeII ACGT 1 cut(s) 183
MaeIII GTNAC 3 cut(s) 754, 1097, 1343
MboII GAAGA 4 cut(s) 148, 190, 250, 449
MflI RGATCY 4 cut(s) 87, 363, 644, 1270
MhlI GDGCHC 6 cut(s) 69, 216, 396, 591, 742, 1043
MlsI TGGCCA 2 cut(s) 318, 908
MluNI TGGCCA 2 cut(s) 318, 908
Mly113I GGCGCC 2 cut(s) 171, 1308
MlyI GAGTC 1 cut(s) 1322
MmeI TCCRAC 3 cut(s) 28, 689, 1161
Mox20I TGGCCA 2 cut(s) 318, 908
MroI TCCGGA 1 cut(s) 366
MroNI GCCGGC 1 cut(s) 173
MscI TGGCCA 2 cut(s) 318, 908
MslI CAYNNNNRTG 4 cut(s) 594, 596, 1148, 1221
Msp20I TGGCCA 2 cut(s) 318, 908
MspA1I CMGCKG 1 cut(s) 132
MspI CCGG 9 cut(s) 36, 95, 168, 174, 240, 282, 367, 390, 435
MspR9I CCNGG 8 cut(s) 32, 44, 391, 398, 407, 436, 650, 1092
Mva1269I GAATGC 1 cut(s) 991
MvaI CCWGG 6 cut(s) 32, 44, 398, 407, 650, 1092
MvnI CGCG 2 cut(s) 132, 192
MwoI GCNNNNNNNGC 4 cut(s) 839, 995, 1205, 1316
NaeI GCCGGC 1 cut(s) 175
NarI GGCGCC 2 cut(s) 171, 1308
NciI CCSGG 2 cut(s) 391, 436
NcoI CCATGG 1 cut(s) 925
NgoMIV GCCGGC 1 cut(s) 173
NlaIII CATG 8 cut(s) 86, 805, 851, 904, 929, 940, 1132, 1240
NmeAIII GCCGAG 2 cut(s) 135, 1336
NmuCI GTSAC 3 cut(s) 754, 1097, 1343
OliI CACNNNNGTG 2 cut(s) 596, 1148
PaeR7I CTCGAG 2 cut(s) 77, 209
PciSI GCTCTTC 1 cut(s) 263
PcsI WCGNNNNNNNCGW 3 cut(s) 132, 207, 216
PctI GAATGC 1 cut(s) 991
PdiI GCCGGC 1 cut(s) 175
PfeI GAWTC 3 cut(s) 39, 504, 793
PflFI GACNNNGTC 1 cut(s) 230
PinAI ACCGGT 1 cut(s) 239
PkrI GCNGC 9 cut(s) 131, 134, 273, 298, 609, 641, 855, 991, 1279
PleI GAGTC 1 cut(s) 1321
PluTI GGCGCC 2 cut(s) 174, 1311
PpsI GAGTC 1 cut(s) 1321
Psp124BI GAGCTC 1 cut(s) 69
Psp6I CCWGG 6 cut(s) 30, 42, 396, 405, 648, 1090
PspEI GGTNACC 1 cut(s) 1097
PspFI CCCAGC 1 cut(s) 60
PspGI CCWGG 6 cut(s) 30, 42, 396, 405, 648, 1090
PspPI GGNCC 2 cut(s) 232, 1261
PspXI VCTCGAGB 2 cut(s) 77, 209
PstNI CAGNNNCTG 1 cut(s) 866
PsuI RGATCY 4 cut(s) 87, 363, 644, 1270
PsyI GACNNNGTC 1 cut(s) 230
RsaI GTAC 6 cut(s) 244, 433, 935, 956, 1018, 1352
RsaNI GTAC 6 cut(s) 243, 432, 934, 955, 1017, 1351
RseI CAYNNNNRTG 4 cut(s) 594, 596, 1148, 1221
SacI GAGCTC 1 cut(s) 69
SacII CCGCGG 1 cut(s) 133
SalI GTCGAC 1 cut(s) 203
SapI GCTCTTC 1 cut(s) 263
SatI GCNGC 9 cut(s) 130, 133, 272, 297, 608, 640, 854, 990, 1278
Sau96I GGNCC 2 cut(s) 232, 1261
ScaI AGTACT 1 cut(s) 1352
SchI GAGTC 1 cut(s) 1322
ScrFI CCNGG 8 cut(s) 32, 44, 391, 398, 407, 436, 650, 1092
SduI GDGCHC 6 cut(s) 69, 216, 396, 591, 742, 1043
SfaNI GCATC 1 cut(s) 96
SfoI GGCGCC 2 cut(s) 172, 1309
Sfr274I CTCGAG 2 cut(s) 77, 209
Sfr303I CCGCGG 1 cut(s) 133
SgrBI CCGCGG 1 cut(s) 133
SinI GGWCC 1 cut(s) 232
SlaI CTCGAG 2 cut(s) 77, 209
SmiMI CAYNNNNRTG 4 cut(s) 594, 596, 1148, 1221
SmlI CTYRAG 3 cut(s) 77, 209, 584
SmoI CTYRAG 3 cut(s) 77, 209, 584
SspDI GGCGCC 2 cut(s) 170, 1307
SstI GAGCTC 1 cut(s) 69
StyD4I CCNGG 8 cut(s) 30, 42, 389, 396, 405, 434, 648, 1090
StyI CCWWGG 1 cut(s) 925
TaaI ACNGT 6 cut(s) 124, 220, 511, 560, 881, 1084
TaiI ACGT 1 cut(s) 186
TaqI TCGA 8 cut(s) 17, 78, 204, 210, 225, 345, 732, 1173
TatI WGTACW 2 cut(s) 933, 1350
TauI GCSGC 5 cut(s) 132, 135, 299, 642, 992
TfiI GAWTC 3 cut(s) 39, 504, 793
TscAI CASTG 6 cut(s) 514, 565, 867, 886, 1087, 1153
TseFI GTSAC 3 cut(s) 754, 1097, 1343
TseI GCWGC 4 cut(s) 271, 607, 853, 1277
Tsp45I GTSAC 3 cut(s) 754, 1097, 1343
TspDTI ATGAA 4 cut(s) 696, 785, 790, 1353
TspRI CASTG 6 cut(s) 514, 565, 867, 886, 1087, 1153
Tth111I GACNNNGTC 1 cut(s) 230
VpaK11BI GGWCC 1 cut(s) 232
XagI CCTNNNNNAGG 2 cut(s) 724, 1106
XcmI CCANNNNNNNNNTGG 2 cut(s) 541, 1027
XhoI CTCGAG 2 cut(s) 77, 209
XmiI GTMKAC 1 cut(s) 204
ZrmI AGTACT 1 cut(s) 1352
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.