Rroxscaffold_3G00273310

40S ribosomal protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0004882
Physical Location & Seq
Forward (+)
65122067 .. 65123763
1697 bp
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UTR
Exon/CDS
Intron
Rroxscaffold_3G00273310.1

Sequence Viewer

Length: 564 bp
ATGAGATTCACAATTGAACATTGCTTTATCCGTACTTTTAAATGCATTGAACCAGAAGAGATGGCTAGTCGCATCAACCTCTTAAACCGAGAAGACAGAGACGGCAATGATACTGACTTCATCATCAAGCCAGAATATGATTTTCTCGAGATCGACCAGCCAAATTATGCAAATATTGAAGAAGAAAATATGTCCTTACAAGGTGGACCTCATTTCAAAAACTTCGTGGACTTATTGAGAGATTTGTTGGCAATGGCATTGGCCAATGGTTTGCTCAGAAGAGGACTTCCCACAGCTTCCAATCTCATTCCGGACCAGAACGTATGGCTGTGTGTTTTTGCTGAGGACAGCAACCAGGATGGTTTTGTCGAGGCGGTAAAGTCATTGTGTGAGGAGTATCATGTTAAGTATCTGACAATTGCCAGTGCTAAAACTCTTGGACAGTGGTCTGTGGAAGGCATATATGAGACGGAACTTCATAAAGCAGTTTTGGAGGTCTGGAAGTCTCTCACATCAAAGCTGGAAGTGCTGGAAGAGTCTGGATTGTGTCGCCCTTTTGTCTAG

Protein Analysis

187

Amino Acids

21.48

Weight (kDa)

4.66

Isoelectric Point (pI)

48.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L7Ae PF01248 80 - 150 3.7e-08 Ribosomal protein L7Ae/L30e/S12e/Gadd45 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 310
AciI CCGC 1 cut(s) 374
AcoI YGGCCR 1 cut(s) 261
AfaI GTAC 1 cut(s) 34
AgsI TTSAA 4 cut(s) 17, 50, 179, 217
AjnI CCWGG 1 cut(s) 354
AluBI AGCT 2 cut(s) 296, 520
AluI AGCT 2 cut(s) 296, 520
Alw26I GTCTC 3 cut(s) 93, 461, 510
Ama87I CYCGRG 1 cut(s) 146
Aor13HI TCCGGA 1 cut(s) 310
AoxI GGCC 1 cut(s) 261
AspS9I GGNCC 2 cut(s) 206, 313
AvaI CYCGRG 1 cut(s) 146
AvaII GGWCC 2 cut(s) 206, 313
BalI TGGCCA 1 cut(s) 263
BbsI GAAGAC 1 cut(s) 99
BbvCI CCTCAGC 1 cut(s) 342
BccI CCATC 2 cut(s) 55, 353
BceAI ACGGC 1 cut(s) 118
BciT130I CCWGG 1 cut(s) 356
BcoDI GTCTC 3 cut(s) 93, 461, 510
BfaI CTAG 2 cut(s) 66, 562
Bme1390I CCNGG 1 cut(s) 356
Bme18I GGWCC 2 cut(s) 206, 313
BmeT110I CYCGRG 1 cut(s) 146
BmgT120I GGNCC 2 cut(s) 206, 313
BmrFI CCNGG 1 cut(s) 356
BmsI GCATC 1 cut(s) 81
BoxI GACNNNNGTC 1 cut(s) 445
BpiI GAAGAC 1 cut(s) 99
Bpu10I CCTNAGC 1 cut(s) 342
BsaWI WCCGGW 1 cut(s) 310
Bse1I ACTGG 1 cut(s) 423
Bse3DI GCAATG 3 cut(s) 19, 112, 258
BseAI TCCGGA 1 cut(s) 310
BseBI CCWGG 1 cut(s) 356
BseGI GGATG 1 cut(s) 364
BseMI GCAATG 3 cut(s) 19, 112, 258
BseMII CTCAG 2 cut(s) 289, 333
BseNI ACTGG 1 cut(s) 423
BseRI GAGGAG 1 cut(s) 407
BshFI GGCC 1 cut(s) 263
BsiHKCI CYCGRG 1 cut(s) 146
BsiSI CCGG 1 cut(s) 311
BsmAI GTCTC 3 cut(s) 93, 461, 510
BsmBI CGTCTC 2 cut(s) 93, 461
BsnI GGCC 1 cut(s) 263
BsoBI CYCGRG 1 cut(s) 146
Bsp13I TCCGGA 1 cut(s) 310
Bsp143I GATC 1 cut(s) 150
BspACI CCGC 1 cut(s) 374
BspANI GGCC 1 cut(s) 263
BspCNI CTCAG 2 cut(s) 288, 334
BspEI TCCGGA 1 cut(s) 310
BsrDI GCAATG 3 cut(s) 19, 112, 258
BsrI ACTGG 1 cut(s) 423
BssMI GATC 1 cut(s) 150
Bst2UI CCWGG 1 cut(s) 356
Bst4CI ACNGT 1 cut(s) 444
Bst6I CTCTTC 3 cut(s) 51, 274, 528
BstDEI CTNAG 2 cut(s) 275, 342
BstF5I GGATG 1 cut(s) 364
BstKTI GATC 1 cut(s) 153
BstMAI GTCTC 3 cut(s) 93, 461, 510
BstMBI GATC 1 cut(s) 150
BstMWI GCNNNNNNNGC 1 cut(s) 526
BstNI CCWGG 1 cut(s) 356
BstPAI GACNNNNGTC 1 cut(s) 445
BstSCI CCNGG 1 cut(s) 354
BstV2I GAAGAC 1 cut(s) 99
BsuRI GGCC 1 cut(s) 263
BtsCI GGATG 1 cut(s) 364
BtsIMutI CAGTG 2 cut(s) 430, 449
Cfr13I GGNCC 2 cut(s) 206, 313
Csp6I GTAC 1 cut(s) 33
CviAII CATG 1 cut(s) 401
CviJI RGCY 7 cut(s) 65, 130, 160, 263, 296, 328, 520
CviKI_1 RGCY 7 cut(s) 65, 130, 160, 263, 296, 328, 520
CviQI GTAC 1 cut(s) 33
DdeI CTNAG 2 cut(s) 275, 342
DpnI GATC 1 cut(s) 152
DpnII GATC 1 cut(s) 150
DraI TTTAAA 1 cut(s) 40
EaeI YGGCCR 1 cut(s) 261
Eam1104I CTCTTC 3 cut(s) 51, 274, 528
EarI CTCTTC 3 cut(s) 51, 274, 528
Eco47I GGWCC 2 cut(s) 206, 313
Eco88I CYCGRG 1 cut(s) 146
EcoRII CCWGG 1 cut(s) 354
EcoT22I ATGCAT 1 cut(s) 47
Esp3I CGTCTC 2 cut(s) 93, 461
FaeI CATG 1 cut(s) 404
FaiI YATR 9 cut(s) 138, 168, 191, 325, 402, 461, 463, 465, 480
FatI CATG 1 cut(s) 400
FokI GGATG 1 cut(s) 371
FspBI CTAG 2 cut(s) 66, 562
HaeIII GGCC 1 cut(s) 263
HapII CCGG 1 cut(s) 311
Hin1II CATG 1 cut(s) 404
HinfI GANTC 2 cut(s) 6, 536
HpaII CCGG 1 cut(s) 311
Hpy166II GTNNAC 2 cut(s) 206, 229
Hpy188I TCNGA 2 cut(s) 278, 414
Hpy188III TCNNGA 5 cut(s) 146, 148, 311, 499, 540
Hpy8I GTNNAC 2 cut(s) 206, 229
HpyAV CCTTC 1 cut(s) 449
HpyCH4III ACNGT 1 cut(s) 444
HpyCH4IV ACGT 1 cut(s) 321
HpyCH4V TGCA 2 cut(s) 45, 170
HpyF10VI GCNNNNNNNGC 1 cut(s) 526
HpyF3I CTNAG 2 cut(s) 275, 342
HpySE526I ACGT 1 cut(s) 321
Hsp92II CATG 1 cut(s) 404
Kpn2I TCCGGA 1 cut(s) 310
Kzo9I GATC 1 cut(s) 150
LweI GCATC 1 cut(s) 81
MaeI CTAG 2 cut(s) 66, 562
MaeII ACGT 1 cut(s) 321
MalI GATC 1 cut(s) 152
MboI GATC 1 cut(s) 150
MboII GAAGA 6 cut(s) 68, 104, 191, 194, 291, 545
MfeI CAATTG 2 cut(s) 12, 417
MlsI TGGCCA 1 cut(s) 263
MluCI AATT 3 cut(s) 12, 163, 417
MluNI TGGCCA 1 cut(s) 263
MlyI GAGTC 1 cut(s) 545
MnlI CCTC 7 cut(s) 89, 219, 275, 337, 364, 385, 487
Mox20I TGGCCA 1 cut(s) 263
Mph1103I ATGCAT 1 cut(s) 47
MroI TCCGGA 1 cut(s) 310
MscI TGGCCA 1 cut(s) 263
MseI TTAA 3 cut(s) 39, 83, 405
Msp20I TGGCCA 1 cut(s) 263
MspI CCGG 1 cut(s) 311
MspR9I CCNGG 1 cut(s) 356
MunI CAATTG 2 cut(s) 12, 417
MvaI CCWGG 1 cut(s) 356
MwoI GCNNNNNNNGC 1 cut(s) 526
NdeII GATC 1 cut(s) 150
NlaIII CATG 1 cut(s) 404
NsiI ATGCAT 1 cut(s) 47
PaeR7I CTCGAG 1 cut(s) 146
PfeI GAWTC 1 cut(s) 6
PleI GAGTC 1 cut(s) 544
PpsI GAGTC 1 cut(s) 544
PshAI GACNNNNGTC 1 cut(s) 445
Psp6I CCWGG 1 cut(s) 354
PspGI CCWGG 1 cut(s) 354
PspPI GGNCC 2 cut(s) 206, 313
RsaI GTAC 1 cut(s) 34
RsaNI GTAC 1 cut(s) 33
SaqAI TTAA 3 cut(s) 39, 83, 405
Sau3AI GATC 1 cut(s) 150
Sau96I GGNCC 2 cut(s) 206, 313
SchI GAGTC 1 cut(s) 545
ScrFI CCNGG 1 cut(s) 356
SetI ASST 7 cut(s) 81, 205, 211, 298, 324, 498, 522
SfaNI GCATC 1 cut(s) 81
Sfr274I CTCGAG 1 cut(s) 146
SinI GGWCC 2 cut(s) 206, 313
SlaI CTCGAG 1 cut(s) 146
SmlI CTYRAG 1 cut(s) 146
SmoI CTYRAG 1 cut(s) 146
Sse9I AATT 3 cut(s) 12, 163, 417
SsiI CCGC 1 cut(s) 374
SspI AATATT 1 cut(s) 175
SspMI CTAG 2 cut(s) 66, 562
StyD4I CCNGG 1 cut(s) 354
TaaI ACNGT 1 cut(s) 444
TaiI ACGT 1 cut(s) 324
TaqI TCGA 3 cut(s) 147, 153, 369
TasI AATT 3 cut(s) 12, 163, 417
TfiI GAWTC 1 cut(s) 6
Tru1I TTAA 3 cut(s) 39, 83, 405
Tru9I TTAA 3 cut(s) 39, 83, 405
TscAI CASTG 2 cut(s) 430, 449
TspDTI ATGAA 2 cut(s) 109, 467
TspGWI ACGGA 2 cut(s) 20, 485
TspRI CASTG 2 cut(s) 430, 449
VpaK11BI GGWCC 2 cut(s) 206, 313
XhoI CTCGAG 1 cut(s) 146
XspI CTAG 2 cut(s) 66, 562
Zsp2I ATGCAT 1 cut(s) 47
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.