Rh4DG018400

40S ribosomal protein S12-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0004882
Physical Location & Seq
Forward (+)
2879369 .. 2890155
10787 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG018400.1

Sequence Viewer

Length: 1611 bp
ATGGGGACGGCCCGCACTTTGGATCGTTGTATGAATTTAATATATGCATGTAAGCAAGACTACTTTCGACCTTTAGCACAGTTCAGGGATGATGATGTTAGCCTTCCGTTAAGTTGGAAATTTTGCTCCTTGGATACCAACAACATATGTCTATTGCCAGATAGTGGTCCTTTACCTCCTGATGATGTGGTGAACCCCTTGTCTGGATATATGTTCAATGTTGATACCAATAAATGTCATTTAATCAAGCCGCCCGTTGCTCCCAAATGGGAAGGACCTGTTGTATCTGCATGTGGGAAACTTTACTCTATTACGGATCCATACTGCTCATTTGATGTGCCAGAGCCCTCTTTCGAGCGATATGATCCTTATAGGTGTTGTTGGGAAACTCGGAAATCTTTTCCTTATGGTAAAAATTGTTCCGAACAAAGGATTGGGGGTCATGCCGTTTGTGATGGACTCATCCTGTATAATATAATCATTCCAGGGGATAATCCATTCTTTTTCTCTAGAAAATATCAAGATGAAATGTGGGCTTATGATGTAACACTGGATGAATGGCTTCCAGTTAGGGTCGAGAACACAGATTGCTATAGTTTCCGAGGACCGGCTGTATGTGTGGACAAGACTATCTATGCGTTATCAAGCAGCTTGGCGGTTATGGCACTCTCTATCACAAGGCATGAAAATCTTGTTACCTGCTCTCTAAAGTCACAGTTTGTTTGTCCAGAGTTTGGGAACTTTCGACCAGGTGTACAGAGAATGGAGCGTTTGATTTATTTGGGTGACCTGAACTTTTGTCTTGTTCAGACTGGATTTCACTATGACAGTAGATCCAGTCAATCTCTTAAAATTACCACATTTCACCTTGTGGGAGAAACCAATATCAAAATCGAACGTTCATTCATTTATGAGGTGCCTCTTGAGGGTCATAAGAGATTCACAATTGGACATTGCTTTATCCATACTTTTAAACGCATTGAACTAGAAGAGTTGGCTAGTCGCATCGACTTCTTAAACCAAGAATACAGATTCGGCATTCATGCTGAATTCATCATCAAGCCTGTTGACTTCATATTCAAGCCAGAATATGATTATCTCGAGATCGACCAGCCAAATTATGCAAATATTGAAGAAGAAAATATATCCTTACAAGGTGGACCTCATTTCAAAAACTTCGTGGACTTGTTGAAAGATTTGTTGGCAATGGCATTGGCCAATGGATTGCTCAGAAGAGGAATTCCCACAGCTTCCAATCTCATTCCGGACCAGAAGGTATGGCTGTGTGTTTTTGCTGAGGACAGCAACCAGGATGATGTTGTCAAGGCGGTAAAGTCATTGTGTGAGGGGTATCATGTTAAGTATCTGACAATTGCCAGTGCTAAAACTCTTGGACAGTGGTCTCTGTCTGGTAATAAGGATTCTGAAGGAAAGGCAAAGAGGGTGGTTCGTTGCTCTTGTGTTGTTATTGAGGAAGGCATATATGAGACGGAACTTCATAAAGCAGTTTTGGAAGTCTGGAAGTCTCTCCCATCAAAGCTGGAAGTGCTGGAAGAGTCTGGACAGTGTCGCCCTTTTGTCGGGTCAAGGGCTTCTTTAGATAGTCCATGA

Protein Analysis

536

Amino Acids

61.05

Weight (kDa)

5.51

Isoelectric Point (pI)

47.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L7Ae PF01248 398 - 489 2.4e-09 Ribosomal protein L7Ae/L30e/S12e/Gadd45 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 707
AccB1I GGYRCC 1 cut(s) 916
AccB7I CCANNNNNTGG 2 cut(s) 164, 734
AccIII TCCGGA 1 cut(s) 1264
AciI CCGC 4 cut(s) 13, 251, 656, 1328
AclI AACGTT 1 cut(s) 898
AclWI GGATC 5 cut(s) 30, 311, 324, 359, 828
AcoI YGGCCR 1 cut(s) 1215
AcsI RAATTY 4 cut(s) 34, 119, 1049, 1239
AcuI CTGAAG 1 cut(s) 1446
AdeI CACNNNGTG 1 cut(s) 871
AfaI GTAC 1 cut(s) 756
AfiI CCNNNNNNNGG 8 cut(s) 19, 164, 203, 429, 607, 734, 926, 1580
AgsI TTSAA 6 cut(s) 217, 983, 1081, 1133, 1171, 1192
AjnI CCWGG 3 cut(s) 484, 748, 1308
AjuI GAANNNNNNNTTGG 4 cut(s) 417, 449, 1184, 1216
AluBI AGCT 3 cut(s) 651, 1250, 1540
AluI AGCT 3 cut(s) 651, 1250, 1540
Alw26I GTCTC 3 cut(s) 1407, 1481, 1530
AlwI GGATC 5 cut(s) 30, 311, 324, 359, 828
Ama87I CYCGRG 1 cut(s) 1100
Aor13HI TCCGGA 1 cut(s) 1264
AoxI GGCC 2 cut(s) 9, 1215
ApeKI GCWGC 1 cut(s) 648
ApoI RAATTY 4 cut(s) 34, 119, 1049, 1239
Asp700I GAANNNNTTC 1 cut(s) 561
AspS9I GGNCC 6 cut(s) 10, 167, 275, 605, 1160, 1267
AsuHPI GGTGA 3 cut(s) 202, 797, 857
AvaI CYCGRG 1 cut(s) 1100
AvaII GGWCC 5 cut(s) 167, 275, 605, 1160, 1267
BalI TGGCCA 1 cut(s) 1217
BamHI GGATCC 1 cut(s) 316
BanI GGYRCC 1 cut(s) 916
BanII GRGCYC 1 cut(s) 348
BbvCI CCTCAGC 1 cut(s) 1296
BbvI GCAGC 1 cut(s) 660
BccI CCATC 2 cut(s) 449, 1540
BceAI ACGGC 2 cut(s) 24, 431
BciT130I CCWGG 3 cut(s) 486, 750, 1310
BciVI GTATCC 1 cut(s) 127
BcoDI GTCTC 3 cut(s) 1407, 1481, 1530
BfaI CTAG 3 cut(s) 510, 986, 999
BfmI CTRYAG 1 cut(s) 592
BfuAI ACCTGC 1 cut(s) 707
BfuI GTATCC 1 cut(s) 127
BisI GCNGC 2 cut(s) 251, 649
BlsI GCNGC 2 cut(s) 252, 650
Bme1390I CCNGG 3 cut(s) 486, 750, 1310
Bme18I GGWCC 5 cut(s) 167, 275, 605, 1160, 1267
BmeT110I CYCGRG 1 cut(s) 1100
BmgT120I GGNCC 6 cut(s) 10, 167, 275, 605, 1160, 1267
BmiI GGNNCC 2 cut(s) 318, 918
BmrFI CCNGG 3 cut(s) 486, 750, 1310
BmsI GCATC 1 cut(s) 1014
BoxI GACNNNNGTC 1 cut(s) 1399
Bpu10I CCTNAGC 1 cut(s) 1296
BpuEI CTTGAG 1 cut(s) 944
BsaI GGTCTC 1 cut(s) 1407
BsaJI CCNNGG 3 cut(s) 129, 485, 601
BsaWI WCCGGW 1 cut(s) 1264
Bsc4I CCNNNNNNNGG 8 cut(s) 19, 164, 203, 429, 607, 734, 926, 1580
Bse118I RCCGGY 1 cut(s) 607
Bse1I ACTGG 5 cut(s) 555, 566, 817, 837, 1377
Bse3DI GCAATG 2 cut(s) 952, 1212
BseAI TCCGGA 1 cut(s) 1264
BseBI CCWGG 3 cut(s) 486, 750, 1310
BseDI CCNNGG 3 cut(s) 129, 485, 601
BseGI GGATG 4 cut(s) 94, 462, 559, 1318
BseLI CCNNNNNNNGG 8 cut(s) 19, 164, 203, 429, 607, 734, 926, 1580
BseMI GCAATG 2 cut(s) 952, 1212
BseMII CTCAG 2 cut(s) 1243, 1287
BseNI ACTGG 5 cut(s) 555, 566, 817, 837, 1377
BseXI GCAGC 1 cut(s) 660
BshFI GGCC 2 cut(s) 11, 1217
BshNI GGYRCC 1 cut(s) 916
BsiHKCI CYCGRG 1 cut(s) 1100
BsiSI CCGG 2 cut(s) 608, 1265
BslFI GGGAC 1 cut(s) 19
BslI CCNNNNNNNGG 8 cut(s) 19, 164, 203, 429, 607, 734, 926, 1580
BsmAI GTCTC 3 cut(s) 1407, 1481, 1530
BsmBI CGTCTC 1 cut(s) 1481
BsmFI GGGAC 1 cut(s) 19
BsmI GAATGC 1 cut(s) 1038
BsnI GGCC 2 cut(s) 11, 1217
Bso31I GGTCTC 1 cut(s) 1407
BsoBI CYCGRG 1 cut(s) 1100
Bsp1286I GDGCHC 1 cut(s) 348
Bsp13I TCCGGA 1 cut(s) 1264
Bsp1407I TGTACA 1 cut(s) 754
Bsp143I GATC 5 cut(s) 22, 316, 364, 833, 1104
BspACI CCGC 4 cut(s) 13, 251, 656, 1328
BspANI GGCC 2 cut(s) 11, 1217
BspCNI CTCAG 2 cut(s) 1242, 1288
BspEI TCCGGA 1 cut(s) 1264
BspLI GGNNCC 2 cut(s) 318, 918
BspMI ACCTGC 1 cut(s) 707
BspPI GGATC 5 cut(s) 30, 311, 324, 359, 828
BspT107I GGYRCC 1 cut(s) 916
BspTNI GGTCTC 1 cut(s) 1407
BsrDI GCAATG 2 cut(s) 952, 1212
BsrFI RCCGGY 1 cut(s) 607
BsrGI TGTACA 1 cut(s) 754
BsrI ACTGG 5 cut(s) 555, 566, 817, 837, 1377
BssAI RCCGGY 1 cut(s) 607
BssECI CCNNGG 3 cut(s) 129, 485, 601
BssMI GATC 5 cut(s) 22, 316, 364, 833, 1104
BssT1I CCWWGG 1 cut(s) 129
Bst2UI CCWGG 3 cut(s) 486, 750, 1310
Bst4CI ACNGT 5 cut(s) 81, 717, 830, 1398, 1566
Bst6I CTCTTC 3 cut(s) 984, 1228, 1548
BstAUI TGTACA 1 cut(s) 754
BstC8I GCNNGC 1 cut(s) 13
BstDEI CTNAG 2 cut(s) 1229, 1296
BstEII GGTNACC 1 cut(s) 785
BstF5I GGATG 4 cut(s) 94, 462, 559, 1318
BstKTI GATC 5 cut(s) 25, 319, 367, 836, 1107
BstMAI GTCTC 3 cut(s) 1407, 1481, 1530
BstMBI GATC 5 cut(s) 22, 316, 364, 833, 1104
BstMWI GCNNNNNNNGC 2 cut(s) 662, 1546
BstNI CCWGG 3 cut(s) 486, 750, 1310
BstNSI RCATGY 2 cut(s) 51, 294
BstPAI GACNNNNGTC 1 cut(s) 1399
BstPI GGTNACC 1 cut(s) 785
BstSCI CCNGG 3 cut(s) 484, 748, 1308
BstSFI CTRYAG 1 cut(s) 592
BstV1I GCAGC 1 cut(s) 660
BstX2I RGATCY 2 cut(s) 316, 833
BstYI RGATCY 2 cut(s) 316, 833
BsuI GTATCC 1 cut(s) 127
BsuRI GGCC 2 cut(s) 11, 1217
BtsCI GGATG 4 cut(s) 94, 462, 559, 1318
BtsIMutI CAGTG 4 cut(s) 548, 1384, 1403, 1571
BveI ACCTGC 1 cut(s) 707
Cac8I GCNNGC 1 cut(s) 13
Cfr10I RCCGGY 1 cut(s) 607
Cfr13I GGNCC 6 cut(s) 10, 167, 275, 605, 1160, 1267
CsiI ACCWGGT 1 cut(s) 748
Csp6I GTAC 1 cut(s) 755
CspCI CAANNNNNGTGG 2 cut(s) 1425, 1460
CviAII CATG 7 cut(s) 48, 291, 443, 683, 1043, 1355, 1608
CviQI GTAC 1 cut(s) 755
DdeI CTNAG 2 cut(s) 1229, 1296
DpnI GATC 5 cut(s) 24, 318, 366, 835, 1106
DpnII GATC 5 cut(s) 22, 316, 364, 833, 1104
DraI TTTAAA 1 cut(s) 973
DraIII CACNNNGTG 1 cut(s) 871
EaeI YGGCCR 1 cut(s) 1215
Eam1104I CTCTTC 3 cut(s) 984, 1228, 1548
EarI CTCTTC 3 cut(s) 984, 1228, 1548
Eco130I CCWWGG 1 cut(s) 129
Eco24I GRGCYC 1 cut(s) 348
Eco31I GGTCTC 1 cut(s) 1407
Eco47I GGWCC 5 cut(s) 167, 275, 605, 1160, 1267
Eco57I CTGAAG 1 cut(s) 1446
Eco88I CYCGRG 1 cut(s) 1100
Eco91I GGTNACC 1 cut(s) 785
EcoO109I RGGNCCY 1 cut(s) 275
EcoO65I GGTNACC 1 cut(s) 785
EcoRI GAATTC 2 cut(s) 1049, 1239
EcoRII CCWGG 3 cut(s) 484, 748, 1308
EcoT14I CCWWGG 1 cut(s) 129
EcoT22I ATGCAT 1 cut(s) 49
EcoT38I GRGCYC 1 cut(s) 348
ErhI CCWWGG 1 cut(s) 129
Esp3I CGTCTC 1 cut(s) 1481
FaeI CATG 7 cut(s) 51, 294, 446, 686, 1046, 1358, 1611
FalI AAGNNNNNCTT 2 cut(s) 1579, 1611
FaqI GGGAC 1 cut(s) 19
FatI CATG 7 cut(s) 47, 290, 442, 682, 1042, 1354, 1607
FauI CCCGC 1 cut(s) 20
FauNDI CATATG 1 cut(s) 146
Fnu4HI GCNGC 2 cut(s) 251, 649
FokI GGATG 4 cut(s) 101, 449, 566, 1325
FriOI GRGCYC 1 cut(s) 348
Fsp4HI GCNGC 2 cut(s) 251, 649
FspBI CTAG 3 cut(s) 510, 986, 999
GluI GCNGC 2 cut(s) 251, 649
HaeIII GGCC 2 cut(s) 11, 1217
HapII CCGG 2 cut(s) 608, 1265
Hin1II CATG 7 cut(s) 51, 294, 446, 686, 1046, 1358, 1611
HincII GTYRAC 1 cut(s) 1069
HindII GTYRAC 1 cut(s) 1069
HinfI GANTC 5 cut(s) 459, 939, 1032, 1421, 1556
HpaII CCGG 2 cut(s) 608, 1265
HphI GGTGA 3 cut(s) 202, 797, 857
Hpy166II GTNNAC 6 cut(s) 193, 622, 755, 1069, 1160, 1183
Hpy188I TCNGA 7 cut(s) 393, 424, 602, 810, 1232, 1368, 1426
Hpy8I GTNNAC 6 cut(s) 193, 622, 755, 1069, 1160, 1183
HpyAV CCTTC 5 cut(s) 113, 266, 1267, 1421, 1469
HpyCH4III ACNGT 5 cut(s) 81, 717, 830, 1398, 1566
HpyCH4IV ACGT 1 cut(s) 898
HpyCH4V TGCA 3 cut(s) 47, 290, 1124
HpyF10VI GCNNNNNNNGC 2 cut(s) 662, 1546
HpyF3I CTNAG 2 cut(s) 1229, 1296
HpySE526I ACGT 1 cut(s) 898
Hsp92II CATG 7 cut(s) 51, 294, 446, 686, 1046, 1358, 1611
Kpn2I TCCGGA 1 cut(s) 1264
Kzo9I GATC 5 cut(s) 22, 316, 364, 833, 1104
LmnI GCTCC 3 cut(s) 131, 265, 766
Lsp1109I GCAGC 1 cut(s) 660
LweI GCATC 1 cut(s) 1014
MabI ACCWGGT 1 cut(s) 748
MaeI CTAG 3 cut(s) 510, 986, 999
MaeII ACGT 1 cut(s) 898
MaeIII GTNAC 4 cut(s) 544, 694, 711, 785
MalI GATC 5 cut(s) 24, 318, 366, 835, 1106
MboI GATC 5 cut(s) 22, 316, 364, 833, 1104
MboII GAAGA 5 cut(s) 1001, 1145, 1148, 1245, 1565
MfeI CAATTG 2 cut(s) 945, 1371
MflI RGATCY 2 cut(s) 316, 833
MhlI GDGCHC 1 cut(s) 348
MlsI TGGCCA 1 cut(s) 1217
MluCI AATT 9 cut(s) 34, 119, 415, 852, 945, 1049, 1117, 1239, 1371
MluNI TGGCCA 1 cut(s) 1217
MlyI GAGTC 2 cut(s) 453, 1565
MmeI TCCRAC 1 cut(s) 95
Mox20I TGGCCA 1 cut(s) 1217
Mph1103I ATGCAT 1 cut(s) 49
MroI TCCGGA 1 cut(s) 1264
MroXI GAANNNNTTC 1 cut(s) 561
MscI TGGCCA 1 cut(s) 1217
MseI TTAA 7 cut(s) 38, 110, 242, 849, 972, 1016, 1359
Msp20I TGGCCA 1 cut(s) 1217
MspI CCGG 2 cut(s) 608, 1265
MspR9I CCNGG 3 cut(s) 486, 750, 1310
MunI CAATTG 2 cut(s) 945, 1371
Mva1269I GAATGC 1 cut(s) 1038
MvaI CCWGG 3 cut(s) 486, 750, 1310
MwoI GCNNNNNNNGC 2 cut(s) 662, 1546
NdeI CATATG 1 cut(s) 146
NdeII GATC 5 cut(s) 22, 316, 364, 833, 1104
NlaIII CATG 7 cut(s) 51, 294, 446, 686, 1046, 1358, 1611
NlaIV GGNNCC 2 cut(s) 318, 918
NmuCI GTSAC 2 cut(s) 711, 785
NsiI ATGCAT 1 cut(s) 49
NspI RCATGY 2 cut(s) 51, 294
PaeR7I CTCGAG 1 cut(s) 1100
PctI GAATGC 1 cut(s) 1038
PdmI GAANNNNTTC 1 cut(s) 561
PfeI GAWTC 3 cut(s) 939, 1032, 1421
PflFI GACNNNGTC 1 cut(s) 1566
PflMI CCANNNNNTGG 2 cut(s) 164, 734
PkrI GCNGC 2 cut(s) 252, 650
PleI GAGTC 2 cut(s) 453, 1564
PpsI GAGTC 2 cut(s) 453, 1564
PpuMI RGGWCCY 1 cut(s) 275
PshAI GACNNNNGTC 1 cut(s) 1399
Psp1406I AACGTT 1 cut(s) 898
Psp5II RGGWCCY 1 cut(s) 275
Psp6I CCWGG 3 cut(s) 484, 748, 1308
PspEI GGTNACC 1 cut(s) 785
PspGI CCWGG 3 cut(s) 484, 748, 1308
PspN4I GGNNCC 2 cut(s) 318, 918
PspPI GGNCC 6 cut(s) 10, 167, 275, 605, 1160, 1267
PspPPI RGGWCCY 1 cut(s) 275
PsrI GAACNNNNNNTAC 2 cut(s) 403, 435
PsuI RGATCY 2 cut(s) 316, 833
PsyI GACNNNGTC 1 cut(s) 1566
RsaI GTAC 1 cut(s) 756
RsaNI GTAC 1 cut(s) 755
SaqAI TTAA 7 cut(s) 38, 110, 242, 849, 972, 1016, 1359
SatI GCNGC 2 cut(s) 251, 649
Sau3AI GATC 5 cut(s) 22, 316, 364, 833, 1104
Sau96I GGNCC 6 cut(s) 10, 167, 275, 605, 1160, 1267
SchI GAGTC 2 cut(s) 453, 1565
ScrFI CCNGG 3 cut(s) 486, 750, 1310
SduI GDGCHC 1 cut(s) 348
SexAI ACCWGGT 1 cut(s) 748
SfaNI GCATC 1 cut(s) 1014
SfcI CTRYAG 1 cut(s) 592
Sfr274I CTCGAG 1 cut(s) 1100
SinI GGWCC 5 cut(s) 167, 275, 605, 1160, 1267
SlaI CTCGAG 1 cut(s) 1100
SmlI CTYRAG 2 cut(s) 923, 1100
SmoI CTYRAG 2 cut(s) 923, 1100
Sse9I AATT 9 cut(s) 34, 119, 415, 852, 945, 1049, 1117, 1239, 1371
SsiI CCGC 4 cut(s) 13, 251, 656, 1328
SspI AATATT 1 cut(s) 1129
SspMI CTAG 3 cut(s) 510, 986, 999
StyD4I CCNGG 3 cut(s) 484, 748, 1308
StyI CCWWGG 1 cut(s) 129
TaaI ACNGT 5 cut(s) 81, 717, 830, 1398, 1566
TaiI ACGT 1 cut(s) 901
TaqI TCGA 8 cut(s) 67, 354, 576, 745, 894, 1008, 1101, 1107
TasI AATT 9 cut(s) 34, 119, 415, 852, 945, 1049, 1117, 1239, 1371
TatI WGTACW 1 cut(s) 754
TauI GCSGC 1 cut(s) 253
TfiI GAWTC 3 cut(s) 939, 1032, 1421
Tru1I TTAA 7 cut(s) 38, 110, 242, 849, 972, 1016, 1359
Tru9I TTAA 7 cut(s) 38, 110, 242, 849, 972, 1016, 1359
TscAI CASTG 4 cut(s) 555, 1384, 1403, 1571
TseFI GTSAC 2 cut(s) 711, 785
TseI GCWGC 1 cut(s) 648
Tsp45I GTSAC 2 cut(s) 711, 785
TspGWI ACGGA 3 cut(s) 96, 329, 1505
TspRI CASTG 4 cut(s) 555, 1384, 1403, 1571
Tth111I GACNNNGTC 1 cut(s) 1566
Van91I CCANNNNNTGG 2 cut(s) 164, 734
VpaK11BI GGWCC 5 cut(s) 167, 275, 605, 1160, 1267
XapI RAATTY 4 cut(s) 34, 119, 1049, 1239
XbaI TCTAGA 1 cut(s) 509
XceI RCATGY 2 cut(s) 51, 294
XhoI CTCGAG 1 cut(s) 1100
XmnI GAANNNNTTC 1 cut(s) 561
XspI CTAG 3 cut(s) 510, 986, 999
Zsp2I ATGCAT 1 cut(s) 49
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.