Rh4CG025500

40S ribosomal protein S12-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0004882
Physical Location & Seq
Reverse (-)
4929719 .. 4934386
4668 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG025500.1

Sequence Viewer

Length: 1719 bp
ATGAGGCGTCGGGGAATCTGGAGTACGATTTCTATTGGAACCCAAAAAAGTGAAGGCAGAGACCATTGTGAGGTCGAAGATGGCTTGGATTTACGGCCAGCAGAGGAATGTCTCGGCTCAGTGGTCGCCTTCGCCTTCGCTTTCGCATTTGAAAACACTGAGCAAGACTACTTTCGACCTTTAGAACAGTTCAGGGATGAGGATGTTAGCCTTCCGTTAAGTTGGAAATTTTGCTCCTTGGATACCAACAACATATGTCTATTGCCAGATAGTGGTCCTTTACCTCCTGATGATGTGGTGAACCCCTTGTCTGGATATATGTTCAATGTTGATACCAATAAATGTCATTTAATCAAGCCGCCCGTTGCTCCCAAATGGAACGGACCTGTTGTATCTGCATGTGGGAAACTTTACTCTATTACAGATCCATGCTGCTCATTTGATGTGCCAGAGCCCTCTTTCGAGCGATATGATCCTTACAGGTGTTGTTGGGAAACTCGGAAATCTTTTCCTTATGGTAAAAATTGTTCCGAACAAAGGATTGGGGGTCATGCCGTTTGTGATGGACTCATCCTGTATAATATAATCATTCCAGGGGATAATCCATTCTTTTTCTCTAGAAAATATCAAGATGAAATGTGGGCTTATGATGTAACACTGGATGAATGGCTTCCAGTTAGGGTCGAGAACACAGATTGCTATAGTTTCCGAGGACCGGCTGTATGTGTGGACAAGACTATCTATGCGTTATCAAGCAGCTTGGCGGTTATGGCACTCTCTATCACAAGGCATGAAAATCTTGTTACCTGCTCTCTAAAGTCACAGTTTGTTTGTCCAGAGTTTGGGAACTTTCGACCAGGTGTACAGAGAATGGAGCGTTTGATTTATTTGGGTGACCTGAACTTTTGTCTTGTTCAGACTGGATTTCACTATGACAGTAGATCCAGTCAATCTCTTAAAATTACCACATTTCACCTTGTGGGAGAAACCAATATCAAAATCGAACGTTCATTCATTTATGAGGTGCCTCTTGAGGGTCATAAGAGATTCACAATTGGACATTGCTTTATCCATACTTTTAAACGCATTGAACTAGAAGAGTTGGCTAGTCGCATCGACTTCTTAAACCAAGAATACAGATTCGGCATTCATGCTGAATTCATCATCAAGCCTGTTGACTTCATATTCAAGCCAGAATATGATTATCTCGAGATCGACCAGCCAAATTATGCAAATATTGAAGAAGAAAATATATCCTTACACGGTGGACCTCATTTCAAAAACTTCGTGGACTTGTTGAAAGATTTGTTGGCAATGGCATTGGCCAATGGATTGCTCAGAAGAGGAATTCCCACAGCTTCCAATCTCATTCCGGACCAGAAGGTATGGCTGTGTGTTTTTGCTGAGGACAGCAGCCAGGATGATGTTGTCAAGGCGGTAAAGTCATTGTGTGAGGGGTATCATGTTAAGTATCTGACAATTGCCAGTGCTAAAACTCTTGGACAGTGGTCTCTGTCTGGTAATAAGGATTCTGAAGGAAAGGCAAAGAGGGTGGTTCGTTGCTCTTGTGTTGTTATTGAGGAAGGCATATATGAGACGGAACTTCATAAAGCAGTTTTGGAAGTCTGGAAGTCTCTCCCATCAAAGCTGGAAGTGCTGGAAGAGTCTGGACAGTGTCGCCCTTTTGTCGGGTCAAGGGCTTCTTTAGATAGTCCATGA

Protein Analysis

572

Amino Acids

65.01

Weight (kDa)

5.29

Isoelectric Point (pI)

49.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L7Ae PF01248 434 - 525 1.8e-09 Ribosomal protein L7Ae/L30e/S12e/Gadd45 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 815
AccB1I GGYRCC 1 cut(s) 1024
AccB7I CCANNNNNTGG 2 cut(s) 272, 842
AccIII TCCGGA 1 cut(s) 1372
AciI CCGC 3 cut(s) 359, 764, 1436
AclI AACGTT 1 cut(s) 1006
AclWI GGATC 3 cut(s) 419, 467, 936
AcoI YGGCCR 2 cut(s) 95, 1323
AcsI RAATTY 3 cut(s) 227, 1157, 1347
AcuI CTGAAG 1 cut(s) 1554
AcyI GRCGYC 1 cut(s) 7
AdeI CACNNNGTG 1 cut(s) 979
AfaI GTAC 2 cut(s) 25, 864
AfiI CCNNNNNNNGG 8 cut(s) 70, 272, 311, 537, 715, 842, 1034, 1688
AgsI TTSAA 7 cut(s) 152, 325, 1091, 1189, 1241, 1279, 1300
AjnI CCWGG 3 cut(s) 592, 856, 1416
AjuI GAANNNNNNNTTGG 4 cut(s) 525, 557, 1292, 1324
AluBI AGCT 3 cut(s) 759, 1358, 1648
AluI AGCT 3 cut(s) 759, 1358, 1648
Alw26I GTCTC 5 cut(s) 54, 116, 1515, 1589, 1638
AlwI GGATC 3 cut(s) 419, 467, 936
Ama87I CYCGRG 1 cut(s) 1208
Aor13HI TCCGGA 1 cut(s) 1372
AoxI GGCC 2 cut(s) 95, 1323
ApeKI GCWGC 3 cut(s) 432, 756, 1413
ApoI RAATTY 3 cut(s) 227, 1157, 1347
Asp700I GAANNNNTTC 1 cut(s) 669
AspS9I GGNCC 5 cut(s) 275, 383, 713, 1268, 1375
AsuHPI GGTGA 3 cut(s) 310, 905, 965
AvaI CYCGRG 1 cut(s) 1208
AvaII GGWCC 5 cut(s) 275, 383, 713, 1268, 1375
BalI TGGCCA 1 cut(s) 1325
BanI GGYRCC 1 cut(s) 1024
BanII GRGCYC 1 cut(s) 456
BbvCI CCTCAGC 1 cut(s) 1404
BbvI GCAGC 3 cut(s) 419, 768, 1425
BccI CCATC 3 cut(s) 74, 557, 1648
BceAI ACGGC 2 cut(s) 110, 539
BciT130I CCWGG 3 cut(s) 594, 858, 1418
BciVI GTATCC 1 cut(s) 235
BcoDI GTCTC 5 cut(s) 54, 116, 1515, 1589, 1638
BfaI CTAG 3 cut(s) 618, 1094, 1107
BfmI CTRYAG 1 cut(s) 700
BfuAI ACCTGC 1 cut(s) 815
BfuI GTATCC 1 cut(s) 235
BisI GCNGC 4 cut(s) 359, 433, 757, 1414
BlsI GCNGC 4 cut(s) 360, 434, 758, 1415
Bme1390I CCNGG 3 cut(s) 594, 858, 1418
Bme18I GGWCC 5 cut(s) 275, 383, 713, 1268, 1375
BmeT110I CYCGRG 1 cut(s) 1208
BmgT120I GGNCC 5 cut(s) 275, 383, 713, 1268, 1375
BmiI GGNNCC 2 cut(s) 40, 1026
BmrFI CCNGG 3 cut(s) 594, 858, 1418
BmsI GCATC 1 cut(s) 1122
BoxI GACNNNNGTC 1 cut(s) 1507
BpmI CTGGAG 1 cut(s) 40
Bpu10I CCTNAGC 1 cut(s) 1404
BpuEI CTTGAG 1 cut(s) 1052
BsaHI GRCGYC 1 cut(s) 7
BsaI GGTCTC 2 cut(s) 54, 1515
BsaJI CCNNGG 3 cut(s) 237, 593, 709
BsaWI WCCGGW 1 cut(s) 1372
Bsc4I CCNNNNNNNGG 8 cut(s) 70, 272, 311, 537, 715, 842, 1034, 1688
Bse118I RCCGGY 1 cut(s) 715
Bse1I ACTGG 5 cut(s) 663, 674, 925, 945, 1485
Bse3DI GCAATG 2 cut(s) 1060, 1320
BseAI TCCGGA 1 cut(s) 1372
BseBI CCWGG 3 cut(s) 594, 858, 1418
BseDI CCNNGG 3 cut(s) 237, 593, 709
BseGI GGATG 5 cut(s) 202, 208, 570, 667, 1426
BseLI CCNNNNNNNGG 8 cut(s) 70, 272, 311, 537, 715, 842, 1034, 1688
BseMI GCAATG 2 cut(s) 1060, 1320
BseMII CTCAG 4 cut(s) 132, 150, 1351, 1395
BseNI ACTGG 5 cut(s) 663, 674, 925, 945, 1485
BseXI GCAGC 3 cut(s) 419, 768, 1425
BshFI GGCC 2 cut(s) 97, 1325
BshNI GGYRCC 1 cut(s) 1024
BsiHKCI CYCGRG 1 cut(s) 1208
BsiSI CCGG 2 cut(s) 716, 1373
BslI CCNNNNNNNGG 8 cut(s) 70, 272, 311, 537, 715, 842, 1034, 1688
BsmAI GTCTC 5 cut(s) 54, 116, 1515, 1589, 1638
BsmBI CGTCTC 1 cut(s) 1589
BsmI GAATGC 1 cut(s) 1146
BsnI GGCC 2 cut(s) 97, 1325
Bso31I GGTCTC 2 cut(s) 54, 1515
BsoBI CYCGRG 1 cut(s) 1208
Bsp1286I GDGCHC 1 cut(s) 456
Bsp13I TCCGGA 1 cut(s) 1372
Bsp1407I TGTACA 1 cut(s) 862
Bsp143I GATC 4 cut(s) 424, 472, 941, 1212
BspACI CCGC 3 cut(s) 359, 764, 1436
BspANI GGCC 2 cut(s) 97, 1325
BspCNI CTCAG 4 cut(s) 131, 151, 1350, 1396
BspEI TCCGGA 1 cut(s) 1372
BspLI GGNNCC 2 cut(s) 40, 1026
BspMI ACCTGC 1 cut(s) 815
BspPI GGATC 3 cut(s) 419, 467, 936
BspT107I GGYRCC 1 cut(s) 1024
BspTNI GGTCTC 2 cut(s) 54, 1515
BsrDI GCAATG 2 cut(s) 1060, 1320
BsrFI RCCGGY 1 cut(s) 715
BsrGI TGTACA 1 cut(s) 862
BsrI ACTGG 5 cut(s) 663, 674, 925, 945, 1485
BssAI RCCGGY 1 cut(s) 715
BssECI CCNNGG 3 cut(s) 237, 593, 709
BssMI GATC 4 cut(s) 424, 472, 941, 1212
BssNI GRCGYC 1 cut(s) 7
BssT1I CCWWGG 1 cut(s) 237
Bst2UI CCWGG 3 cut(s) 594, 858, 1418
Bst4CI ACNGT 6 cut(s) 189, 825, 938, 1265, 1506, 1674
Bst6I CTCTTC 3 cut(s) 1092, 1336, 1656
BstACI GRCGYC 1 cut(s) 7
BstAUI TGTACA 1 cut(s) 862
BstC8I GCNNGC 1 cut(s) 99
BstDEI CTNAG 4 cut(s) 118, 159, 1337, 1404
BstEII GGTNACC 1 cut(s) 893
BstF5I GGATG 5 cut(s) 202, 208, 570, 667, 1426
BstKTI GATC 4 cut(s) 427, 475, 944, 1215
BstMAI GTCTC 5 cut(s) 54, 116, 1515, 1589, 1638
BstMBI GATC 4 cut(s) 424, 472, 941, 1212
BstMWI GCNNNNNNNGC 2 cut(s) 770, 1654
BstNI CCWGG 3 cut(s) 594, 858, 1418
BstNSI RCATGY 1 cut(s) 402
BstPAI GACNNNNGTC 1 cut(s) 1507
BstPI GGTNACC 1 cut(s) 893
BstSCI CCNGG 3 cut(s) 592, 856, 1416
BstSFI CTRYAG 1 cut(s) 700
BstV1I GCAGC 3 cut(s) 419, 768, 1425
BstX2I RGATCY 2 cut(s) 424, 941
BstYI RGATCY 2 cut(s) 424, 941
BsuI GTATCC 1 cut(s) 235
BsuRI GGCC 2 cut(s) 97, 1325
BtsCI GGATG 5 cut(s) 202, 208, 570, 667, 1426
BtsIMutI CAGTG 6 cut(s) 126, 156, 656, 1492, 1511, 1679
BveI ACCTGC 1 cut(s) 815
Cac8I GCNNGC 1 cut(s) 99
Cfr10I RCCGGY 1 cut(s) 715
Cfr13I GGNCC 5 cut(s) 275, 383, 713, 1268, 1375
CsiI ACCWGGT 1 cut(s) 856
Csp6I GTAC 2 cut(s) 24, 863
CspCI CAANNNNNGTGG 2 cut(s) 1533, 1568
CviAII CATG 7 cut(s) 399, 429, 551, 791, 1151, 1463, 1716
CviQI GTAC 2 cut(s) 24, 863
DdeI CTNAG 4 cut(s) 118, 159, 1337, 1404
DpnI GATC 4 cut(s) 426, 474, 943, 1214
DpnII GATC 4 cut(s) 424, 472, 941, 1212
DraI TTTAAA 1 cut(s) 1081
DraIII CACNNNGTG 1 cut(s) 979
EaeI YGGCCR 2 cut(s) 95, 1323
Eam1104I CTCTTC 3 cut(s) 1092, 1336, 1656
EarI CTCTTC 3 cut(s) 1092, 1336, 1656
Eco130I CCWWGG 1 cut(s) 237
Eco24I GRGCYC 1 cut(s) 456
Eco31I GGTCTC 2 cut(s) 54, 1515
Eco47I GGWCC 5 cut(s) 275, 383, 713, 1268, 1375
Eco57I CTGAAG 1 cut(s) 1554
Eco88I CYCGRG 1 cut(s) 1208
Eco91I GGTNACC 1 cut(s) 893
EcoO65I GGTNACC 1 cut(s) 893
EcoRI GAATTC 2 cut(s) 1157, 1347
EcoRII CCWGG 3 cut(s) 592, 856, 1416
EcoT14I CCWWGG 1 cut(s) 237
EcoT38I GRGCYC 1 cut(s) 456
ErhI CCWWGG 1 cut(s) 237
Esp3I CGTCTC 1 cut(s) 1589
FaeI CATG 7 cut(s) 402, 432, 554, 794, 1154, 1466, 1719
FalI AAGNNNNNCTT 2 cut(s) 1687, 1719
FatI CATG 7 cut(s) 398, 428, 550, 790, 1150, 1462, 1715
FauNDI CATATG 1 cut(s) 254
Fnu4HI GCNGC 4 cut(s) 359, 433, 757, 1414
FokI GGATG 5 cut(s) 209, 215, 557, 674, 1433
FriOI GRGCYC 1 cut(s) 456
Fsp4HI GCNGC 4 cut(s) 359, 433, 757, 1414
FspBI CTAG 3 cut(s) 618, 1094, 1107
GluI GCNGC 4 cut(s) 359, 433, 757, 1414
GsuI CTGGAG 1 cut(s) 40
HaeIII GGCC 2 cut(s) 97, 1325
HapII CCGG 2 cut(s) 716, 1373
Hin1I GRCGYC 1 cut(s) 7
Hin1II CATG 7 cut(s) 402, 432, 554, 794, 1154, 1466, 1719
HincII GTYRAC 1 cut(s) 1177
HindII GTYRAC 1 cut(s) 1177
HinfI GANTC 6 cut(s) 15, 567, 1047, 1140, 1529, 1664
HpaII CCGG 2 cut(s) 716, 1373
HphI GGTGA 3 cut(s) 310, 905, 965
Hpy166II GTNNAC 6 cut(s) 301, 730, 863, 1177, 1268, 1291
Hpy188I TCNGA 7 cut(s) 501, 532, 710, 918, 1340, 1476, 1534
Hpy8I GTNNAC 6 cut(s) 301, 730, 863, 1177, 1268, 1291
Hpy99I CGWCG 1 cut(s) 12
HpyAV CCTTC 7 cut(s) 47, 139, 145, 221, 1375, 1529, 1577
HpyCH4III ACNGT 6 cut(s) 189, 825, 938, 1265, 1506, 1674
HpyCH4IV ACGT 1 cut(s) 1006
HpyCH4V TGCA 2 cut(s) 398, 1232
HpyF10VI GCNNNNNNNGC 2 cut(s) 770, 1654
HpyF3I CTNAG 4 cut(s) 118, 159, 1337, 1404
HpySE526I ACGT 1 cut(s) 1006
Hsp92I GRCGYC 1 cut(s) 7
Hsp92II CATG 7 cut(s) 402, 432, 554, 794, 1154, 1466, 1719
Kpn2I TCCGGA 1 cut(s) 1372
Kzo9I GATC 4 cut(s) 424, 472, 941, 1212
LmnI GCTCC 3 cut(s) 239, 373, 874
Lsp1109I GCAGC 3 cut(s) 419, 768, 1425
LweI GCATC 1 cut(s) 1122
MabI ACCWGGT 1 cut(s) 856
MaeI CTAG 3 cut(s) 618, 1094, 1107
MaeII ACGT 1 cut(s) 1006
MaeIII GTNAC 4 cut(s) 652, 802, 819, 893
MalI GATC 4 cut(s) 426, 474, 943, 1214
MboI GATC 4 cut(s) 424, 472, 941, 1212
MboII GAAGA 6 cut(s) 89, 1109, 1253, 1256, 1353, 1673
MfeI CAATTG 2 cut(s) 1053, 1479
MflI RGATCY 2 cut(s) 424, 941
MhlI GDGCHC 1 cut(s) 456
MlsI TGGCCA 1 cut(s) 1325
MluCI AATT 8 cut(s) 227, 523, 960, 1053, 1157, 1225, 1347, 1479
MluNI TGGCCA 1 cut(s) 1325
MlyI GAGTC 2 cut(s) 561, 1673
MmeI TCCRAC 1 cut(s) 203
Mox20I TGGCCA 1 cut(s) 1325
MroI TCCGGA 1 cut(s) 1372
MroXI GAANNNNTTC 1 cut(s) 669
MscI TGGCCA 1 cut(s) 1325
MseI TTAA 6 cut(s) 218, 350, 957, 1080, 1124, 1467
Msp20I TGGCCA 1 cut(s) 1325
MspI CCGG 2 cut(s) 716, 1373
MspR9I CCNGG 3 cut(s) 594, 858, 1418
MunI CAATTG 2 cut(s) 1053, 1479
Mva1269I GAATGC 1 cut(s) 1146
MvaI CCWGG 3 cut(s) 594, 858, 1418
MwoI GCNNNNNNNGC 2 cut(s) 770, 1654
NdeI CATATG 1 cut(s) 254
NdeII GATC 4 cut(s) 424, 472, 941, 1212
NlaIII CATG 7 cut(s) 402, 432, 554, 794, 1154, 1466, 1719
NlaIV GGNNCC 2 cut(s) 40, 1026
NmeAIII GCCGAG 1 cut(s) 93
NmuCI GTSAC 2 cut(s) 819, 893
NspI RCATGY 1 cut(s) 402
PaeR7I CTCGAG 1 cut(s) 1208
PctI GAATGC 1 cut(s) 1146
PdmI GAANNNNTTC 1 cut(s) 669
PfeI GAWTC 4 cut(s) 15, 1047, 1140, 1529
PflFI GACNNNGTC 1 cut(s) 1674
PflMI CCANNNNNTGG 2 cut(s) 272, 842
PkrI GCNGC 4 cut(s) 360, 434, 758, 1415
PleI GAGTC 2 cut(s) 561, 1672
PpsI GAGTC 2 cut(s) 561, 1672
PshAI GACNNNNGTC 1 cut(s) 1507
Psp1406I AACGTT 1 cut(s) 1006
Psp6I CCWGG 3 cut(s) 592, 856, 1416
PspEI GGTNACC 1 cut(s) 893
PspGI CCWGG 3 cut(s) 592, 856, 1416
PspN4I GGNNCC 2 cut(s) 40, 1026
PspPI GGNCC 5 cut(s) 275, 383, 713, 1268, 1375
PsrI GAACNNNNNNTAC 2 cut(s) 511, 543
PsuI RGATCY 2 cut(s) 424, 941
PsyI GACNNNGTC 1 cut(s) 1674
RsaI GTAC 2 cut(s) 25, 864
RsaNI GTAC 2 cut(s) 24, 863
SaqAI TTAA 6 cut(s) 218, 350, 957, 1080, 1124, 1467
SatI GCNGC 4 cut(s) 359, 433, 757, 1414
Sau3AI GATC 4 cut(s) 424, 472, 941, 1212
Sau96I GGNCC 5 cut(s) 275, 383, 713, 1268, 1375
SchI GAGTC 2 cut(s) 561, 1673
ScrFI CCNGG 3 cut(s) 594, 858, 1418
SduI GDGCHC 1 cut(s) 456
SexAI ACCWGGT 1 cut(s) 856
SfaNI GCATC 1 cut(s) 1122
SfcI CTRYAG 1 cut(s) 700
Sfr274I CTCGAG 1 cut(s) 1208
SinI GGWCC 5 cut(s) 275, 383, 713, 1268, 1375
SlaI CTCGAG 1 cut(s) 1208
SmlI CTYRAG 2 cut(s) 1031, 1208
SmoI CTYRAG 2 cut(s) 1031, 1208
Sse9I AATT 8 cut(s) 227, 523, 960, 1053, 1157, 1225, 1347, 1479
SsiI CCGC 3 cut(s) 359, 764, 1436
SspI AATATT 1 cut(s) 1237
SspMI CTAG 3 cut(s) 618, 1094, 1107
StyD4I CCNGG 3 cut(s) 592, 856, 1416
StyI CCWWGG 1 cut(s) 237
TaaI ACNGT 6 cut(s) 189, 825, 938, 1265, 1506, 1674
TaiI ACGT 1 cut(s) 1009
TaqI TCGA 9 cut(s) 75, 175, 462, 684, 853, 1002, 1116, 1209, 1215
TasI AATT 8 cut(s) 227, 523, 960, 1053, 1157, 1225, 1347, 1479
TatI WGTACW 1 cut(s) 862
TauI GCSGC 1 cut(s) 361
TfiI GAWTC 4 cut(s) 15, 1047, 1140, 1529
Tru1I TTAA 6 cut(s) 218, 350, 957, 1080, 1124, 1467
Tru9I TTAA 6 cut(s) 218, 350, 957, 1080, 1124, 1467
TscAI CASTG 6 cut(s) 126, 163, 663, 1492, 1511, 1679
TseFI GTSAC 2 cut(s) 819, 893
TseI GCWGC 3 cut(s) 432, 756, 1413
Tsp45I GTSAC 2 cut(s) 819, 893
TspDTI ATGAA 9 cut(s) 648, 678, 807, 999, 1003, 1139, 1150, 1171, 1595
TspGWI ACGGA 3 cut(s) 204, 396, 1613
TspRI CASTG 6 cut(s) 126, 163, 663, 1492, 1511, 1679
Tth111I GACNNNGTC 1 cut(s) 1674
Van91I CCANNNNNTGG 2 cut(s) 272, 842
VpaK11BI GGWCC 5 cut(s) 275, 383, 713, 1268, 1375
XapI RAATTY 3 cut(s) 227, 1157, 1347
XbaI TCTAGA 1 cut(s) 617
XceI RCATGY 1 cut(s) 402
XhoI CTCGAG 1 cut(s) 1208
XmnI GAANNNNTTC 1 cut(s) 669
XspI CTAG 3 cut(s) 618, 1094, 1107
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.