Rh4AG024400

40S ribosomal protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0004882
Physical Location & Seq
Forward (+)
4743739 .. 4761269
17531 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG024400.1

Sequence Viewer

Length: 519 bp
ATGGAAAAAGAATATGATTATCTCGAGATCGACCAGCCAAATTATGCAAATATTGAAGAAGAAAATATGTCCTTACAAGGTGGACCTCATTTCAAAAACTTCGTGGACGTGTTGAAAGATTTGTTGGCAATGGCATTGGCCAATGGTCTGCTCAGAAGAGGACTTCCCGCAGCTTCCAATCTCATTCCTGACCAGAAGGTATGGCTGTGTGTTTTTGCTGAGGACAGCAACCAGGATGATTTTGTCAAGGCGGTAAAGTCATCGTGTGAGGGGTTTCATGTTAAGTATCTGACAATTGCCAGTGCTAAAACTCTTGGACAGTGGTCTCTGCAATCTTCTTTCGGGAAGGTCAGACATTGCAAAGGGAAGGGACAAAAATTGCTCCAGCTGCTTACTAATATAGGCCATCTCCTCATGCCGGCCTCCAAAATCCTGCCTCATCCAGTTGAGAAAATCCAACCTCGTGCTATAAAGTTTTCCCTACAAACAAAACTGCTCGAAACCTTCACTATCCGTTAG

Protein Analysis

172

Amino Acids

19.37

Weight (kDa)

8.72

Isoelectric Point (pI)

50.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L7Ae PF01248 38 - 111 1.2e-07 Ribosomal protein L7Ae/L30e/S12e/Gadd45 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 168, 251
AcoI YGGCCR 1 cut(s) 138
AfiI CCNNNNNNNGG 1 cut(s) 418
AflIII ACRYGT 1 cut(s) 108
AgsI TTSAA 3 cut(s) 56, 94, 115
AjiI CACGTC 1 cut(s) 109
AjnI CCWGG 1 cut(s) 231
AjuI GAANNNNNNNTTGG 2 cut(s) 107, 139
AluBI AGCT 2 cut(s) 173, 388
AluI AGCT 2 cut(s) 173, 388
Alw26I GTCTC 1 cut(s) 330
Ama87I CYCGRG 1 cut(s) 23
AoxI GGCC 3 cut(s) 138, 403, 420
ApeKI GCWGC 2 cut(s) 170, 388
AspS9I GGNCC 1 cut(s) 83
AvaI CYCGRG 1 cut(s) 23
AvaII GGWCC 1 cut(s) 83
BalI TGGCCA 1 cut(s) 140
BauI CACGAG 1 cut(s) 462
BbvCI CCTCAGC 1 cut(s) 219
BbvI GCAGC 2 cut(s) 182, 375
BccI CCATC 1 cut(s) 414
BciT130I CCWGG 1 cut(s) 233
BcoDI GTCTC 1 cut(s) 330
BisI GCNGC 2 cut(s) 171, 389
BlsI GCNGC 2 cut(s) 172, 390
Bme1390I CCNGG 1 cut(s) 233
Bme18I GGWCC 1 cut(s) 83
BmeT110I CYCGRG 1 cut(s) 23
BmgBI CACGTC 1 cut(s) 109
BmgT120I GGNCC 1 cut(s) 83
BmrFI CCNGG 1 cut(s) 233
BoxI GACNNNNGTC 1 cut(s) 322
BpmI CTGGAG 1 cut(s) 368
Bpu10I CCTNAGC 1 cut(s) 219
BsaI GGTCTC 1 cut(s) 330
Bsc4I CCNNNNNNNGG 1 cut(s) 418
Bse118I RCCGGY 1 cut(s) 418
Bse1I ACTGG 2 cut(s) 300, 443
Bse3DI GCAATG 2 cut(s) 135, 355
BseBI CCWGG 1 cut(s) 233
BseGI GGATG 2 cut(s) 241, 439
BseLI CCNNNNNNNGG 1 cut(s) 418
BseMI GCAATG 2 cut(s) 135, 355
BseMII CTCAG 2 cut(s) 166, 210
BseNI ACTGG 2 cut(s) 300, 443
BseRI GAGGAG 1 cut(s) 401
BseXI GCAGC 2 cut(s) 182, 375
BshFI GGCC 3 cut(s) 140, 405, 422
BsiHKCI CYCGRG 1 cut(s) 23
BsiSI CCGG 1 cut(s) 419
BslFI GGGAC 1 cut(s) 384
BslI CCNNNNNNNGG 1 cut(s) 418
BsmAI GTCTC 1 cut(s) 330
BsmFI GGGAC 1 cut(s) 384
BsnI GGCC 3 cut(s) 140, 405, 422
Bso31I GGTCTC 1 cut(s) 330
BsoBI CYCGRG 1 cut(s) 23
Bsp143I GATC 1 cut(s) 27
BspACI CCGC 2 cut(s) 168, 251
BspANI GGCC 3 cut(s) 140, 405, 422
BspCNI CTCAG 2 cut(s) 165, 211
BspTNI GGTCTC 1 cut(s) 330
BsrDI GCAATG 2 cut(s) 135, 355
BsrFI RCCGGY 1 cut(s) 418
BsrI ACTGG 2 cut(s) 300, 443
BssAI RCCGGY 1 cut(s) 418
BssMI GATC 1 cut(s) 27
BssSI CACGAG 1 cut(s) 462
Bst2BI CACGAG 1 cut(s) 462
Bst2UI CCWGG 1 cut(s) 233
Bst4CI ACNGT 1 cut(s) 321
Bst6I CTCTTC 1 cut(s) 151
BstC8I GCNNGC 1 cut(s) 420
BstDEI CTNAG 2 cut(s) 152, 219
BstF5I GGATG 2 cut(s) 241, 439
BstKTI GATC 1 cut(s) 30
BstMAI GTCTC 1 cut(s) 330
BstMBI GATC 1 cut(s) 27
BstMWI GCNNNNNNNGC 1 cut(s) 388
BstNI CCWGG 1 cut(s) 233
BstPAI GACNNNNGTC 1 cut(s) 322
BstSCI CCNGG 1 cut(s) 231
BstV1I GCAGC 2 cut(s) 182, 375
BsuRI GGCC 3 cut(s) 140, 405, 422
BtrI CACGTC 1 cut(s) 109
BtsCI GGATG 2 cut(s) 241, 439
BtsIMutI CAGTG 2 cut(s) 307, 326
Cac8I GCNNGC 1 cut(s) 420
Cfr10I RCCGGY 1 cut(s) 418
Cfr13I GGNCC 1 cut(s) 83
CviAII CATG 2 cut(s) 278, 415
CviJI RGCY 7 cut(s) 37, 140, 173, 205, 388, 405, 422
CviKI_1 RGCY 7 cut(s) 37, 140, 173, 205, 388, 405, 422
DdeI CTNAG 2 cut(s) 152, 219
DpnI GATC 1 cut(s) 29
DpnII GATC 1 cut(s) 27
EaeI YGGCCR 1 cut(s) 138
Eam1104I CTCTTC 1 cut(s) 151
EarI CTCTTC 1 cut(s) 151
Eco31I GGTCTC 1 cut(s) 330
Eco47I GGWCC 1 cut(s) 83
Eco88I CYCGRG 1 cut(s) 23
EcoRII CCWGG 1 cut(s) 231
FaeI CATG 2 cut(s) 281, 418
FaiI YATR 8 cut(s) 15, 45, 68, 202, 279, 401, 416, 470
FaqI GGGAC 1 cut(s) 384
FatI CATG 2 cut(s) 277, 414
FauI CCCGC 1 cut(s) 175
Fnu4HI GCNGC 2 cut(s) 171, 389
FokI GGATG 2 cut(s) 248, 426
Fsp4HI GCNGC 2 cut(s) 171, 389
GluI GCNGC 2 cut(s) 171, 389
GsuI CTGGAG 1 cut(s) 368
HaeIII GGCC 3 cut(s) 140, 405, 422
HapII CCGG 1 cut(s) 419
Hin1II CATG 2 cut(s) 281, 418
HpaII CCGG 1 cut(s) 419
Hpy166II GTNNAC 2 cut(s) 83, 106
Hpy188I TCNGA 3 cut(s) 155, 291, 353
Hpy188III TCNNGA 4 cut(s) 23, 25, 188, 343
Hpy8I GTNNAC 2 cut(s) 83, 106
HpyAV CCTTC 4 cut(s) 190, 340, 361, 514
HpyCH4III ACNGT 1 cut(s) 321
HpyCH4IV ACGT 1 cut(s) 108
HpyCH4V TGCA 3 cut(s) 47, 331, 360
HpyF10VI GCNNNNNNNGC 1 cut(s) 388
HpyF3I CTNAG 2 cut(s) 152, 219
HpySE526I ACGT 1 cut(s) 108
Hsp92II CATG 2 cut(s) 281, 418
KroI GCCGGC 1 cut(s) 418
KroNI GCCGGC 1 cut(s) 420
Kzo9I GATC 1 cut(s) 27
LmnI GCTCC 1 cut(s) 387
Lsp1109I GCAGC 2 cut(s) 182, 375
MaeII ACGT 1 cut(s) 108
MalI GATC 1 cut(s) 29
MboI GATC 1 cut(s) 27
MboII GAAGA 4 cut(s) 68, 71, 168, 327
MfeI CAATTG 1 cut(s) 294
MlsI TGGCCA 1 cut(s) 140
MluCI AATT 3 cut(s) 40, 294, 377
MluNI TGGCCA 1 cut(s) 140
MmeI TCCRAC 1 cut(s) 481
MnlI CCTC 8 cut(s) 96, 152, 214, 262, 422, 433, 447, 471
Mox20I TGGCCA 1 cut(s) 140
MroNI GCCGGC 1 cut(s) 418
MscI TGGCCA 1 cut(s) 140
MseI TTAA 1 cut(s) 282
Msp20I TGGCCA 1 cut(s) 140
MspA1I CMGCKG 1 cut(s) 388
MspI CCGG 1 cut(s) 419
MspR9I CCNGG 1 cut(s) 233
MunI CAATTG 1 cut(s) 294
MvaI CCWGG 1 cut(s) 233
MwoI GCNNNNNNNGC 1 cut(s) 388
NaeI GCCGGC 1 cut(s) 420
NdeII GATC 1 cut(s) 27
NgoMIV GCCGGC 1 cut(s) 418
NlaIII CATG 2 cut(s) 281, 418
PaeR7I CTCGAG 1 cut(s) 23
PdiI GCCGGC 1 cut(s) 420
PkrI GCNGC 2 cut(s) 172, 390
PshAI GACNNNNGTC 1 cut(s) 322
Psp6I CCWGG 1 cut(s) 231
PspGI CCWGG 1 cut(s) 231
PspPI GGNCC 1 cut(s) 83
PvuII CAGCTG 1 cut(s) 388
SaqAI TTAA 1 cut(s) 282
SatI GCNGC 2 cut(s) 171, 389
Sau3AI GATC 1 cut(s) 27
Sau96I GGNCC 1 cut(s) 83
ScrFI CCNGG 1 cut(s) 233
SetI ASST 9 cut(s) 82, 88, 111, 175, 201, 351, 390, 463, 506
Sfr274I CTCGAG 1 cut(s) 23
SinI GGWCC 1 cut(s) 83
SlaI CTCGAG 1 cut(s) 23
SmlI CTYRAG 1 cut(s) 23
SmoI CTYRAG 1 cut(s) 23
Sse9I AATT 3 cut(s) 40, 294, 377
SsiI CCGC 2 cut(s) 168, 251
SspI AATATT 1 cut(s) 52
StyD4I CCNGG 1 cut(s) 231
TaaI ACNGT 1 cut(s) 321
TaiI ACGT 1 cut(s) 111
TaqI TCGA 3 cut(s) 24, 30, 498
TasI AATT 3 cut(s) 40, 294, 377
Tru1I TTAA 1 cut(s) 282
Tru9I TTAA 1 cut(s) 282
TscAI CASTG 2 cut(s) 307, 326
TseI GCWGC 2 cut(s) 170, 388
TspDTI ATGAA 1 cut(s) 266
TspGWI ACGGA 1 cut(s) 503
TspRI CASTG 2 cut(s) 307, 326
VpaK11BI GGWCC 1 cut(s) 83
XhoI CTCGAG 1 cut(s) 23
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.