Rh7CG030400

40S ribosomal protein S12-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7C
OG0004882
Physical Location & Seq
Reverse (-)
2146829 .. 2148693
1865 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7CG030400.1

Sequence Viewer

Length: 822 bp
ATGGAGCGTTTGATTTATTTGGGTGACCTGAACTTTTGTCTTGTTCAGACTGGATTTCGGTATGACATTAGATCCGGTCAATCTCTTAAAATTACCACATTTCACCTTGTGGGAGAAACCAATATCAAAATCGAACGTTCATTCATTTACGAGGTGCCTCTTGAGGGTTGTATGAGATTCACTATTGAACATTGCTTTTTCCATACTTTTAAACGCATCGAACTCGAAGAGATAACTAGTCGCATCAACTTCTTATACCAAGAAGCCAGATTCGGCATTGATACTGACTTCATCATCAAGCCTCTTGACTTCATCTTCAAGCCAGAATATGATTATCTCGAGATCGACCAGCCAAATTATGCAAATATTGAAGAAGAAAATATGTCCTTACAAGGTGGACCTCATTTCAAAAACTTCGTGGACTTATTGAGAGATTTGTTGGCAATGGCATTGGCCAATGGTTTGCTCAGAAGAGGATTTCCCACAGCTTCCAATCTCATTCCGGACCAGAAGGTATGGCTGTGTGTTTTTGCTGAGGACAGCAACCAGGATGATTTTGTCAAGGGGGTAAAGTCAATGTGTGAGGGGTATCATGTTAAGTATCTGACAATTGCCAGTGCTAAAACTCTTGGACAGTGGTCTGTGTTTGGTAATAAGGATTCTAAAGGAAAGGCAAAGAGGGTGGTTCGTTGCTCTTGCGTTGTTATTGAGGAAGGCATATATGAGACGGAACTTCATAAAGCAGTTTTGGAGGTCTGGAAGTCTCTCCCATCAAAGCTGGAAGTGCTGGAAGAGTCTGGATTGTGTCGCCCTTTTGTCTAG

Protein Analysis

273

Amino Acids

31.54

Weight (kDa)

5.5

Isoelectric Point (pI)

47.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L7Ae PF01248 144 - 233 1.4e-07 Ribosomal protein L7Ae/L30e/S12e/Gadd45 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 154
AccIII TCCGGA 1 cut(s) 502
AclI AACGTT 1 cut(s) 136
AclWI GGATC 1 cut(s) 66
AcoI YGGCCR 1 cut(s) 453
AdeI CACNNNGTG 1 cut(s) 109
AfiI CCNNNNNNNGG 1 cut(s) 164
AgsI TTSAA 4 cut(s) 188, 319, 371, 409
AhlI ACTAGT 1 cut(s) 236
AjnI CCWGG 1 cut(s) 546
AluBI AGCT 2 cut(s) 488, 778
AluI AGCT 2 cut(s) 488, 778
Alw26I GTCTC 2 cut(s) 719, 768
AlwI GGATC 1 cut(s) 66
Ama87I CYCGRG 1 cut(s) 338
Aor13HI TCCGGA 1 cut(s) 502
AoxI GGCC 1 cut(s) 453
AspS9I GGNCC 2 cut(s) 398, 505
AsuHPI GGTGA 2 cut(s) 35, 95
AvaI CYCGRG 1 cut(s) 338
AvaII GGWCC 2 cut(s) 398, 505
BalI TGGCCA 1 cut(s) 455
BanI GGYRCC 1 cut(s) 154
BbvCI CCTCAGC 1 cut(s) 534
BccI CCATC 1 cut(s) 778
BcgI CGANNNNNNTGC 2 cut(s) 205, 239
BciT130I CCWGG 1 cut(s) 548
BcoDI GTCTC 2 cut(s) 719, 768
BcuI ACTAGT 1 cut(s) 236
BfaI CTAG 2 cut(s) 237, 820
Bme1390I CCNGG 1 cut(s) 548
Bme18I GGWCC 2 cut(s) 398, 505
BmeT110I CYCGRG 1 cut(s) 338
BmgT120I GGNCC 2 cut(s) 398, 505
BmiI GGNNCC 1 cut(s) 156
BmrFI CCNGG 1 cut(s) 548
BmsI GCATC 2 cut(s) 225, 252
BoxI GACNNNNGTC 1 cut(s) 637
Bpu10I CCTNAGC 1 cut(s) 534
BpuEI CTTGAG 1 cut(s) 182
BsaWI WCCGGW 2 cut(s) 74, 502
Bsc4I CCNNNNNNNGG 1 cut(s) 164
Bse1I ACTGG 2 cut(s) 55, 615
Bse3DI GCAATG 2 cut(s) 190, 450
BseAI TCCGGA 1 cut(s) 502
BseBI CCWGG 1 cut(s) 548
BseGI GGATG 1 cut(s) 556
BseLI CCNNNNNNNGG 1 cut(s) 164
BseMI GCAATG 2 cut(s) 190, 450
BseMII CTCAG 2 cut(s) 481, 525
BseNI ACTGG 2 cut(s) 55, 615
BshFI GGCC 1 cut(s) 455
BshNI GGYRCC 1 cut(s) 154
BsiHKCI CYCGRG 1 cut(s) 338
BsiSI CCGG 2 cut(s) 75, 503
BslI CCNNNNNNNGG 1 cut(s) 164
BsmAI GTCTC 2 cut(s) 719, 768
BsmBI CGTCTC 1 cut(s) 719
BsnI GGCC 1 cut(s) 455
BsoBI CYCGRG 1 cut(s) 338
Bsp13I TCCGGA 1 cut(s) 502
Bsp143I GATC 2 cut(s) 71, 342
BspANI GGCC 1 cut(s) 455
BspCNI CTCAG 2 cut(s) 480, 526
BspEI TCCGGA 1 cut(s) 502
BspLI GGNNCC 1 cut(s) 156
BspPI GGATC 1 cut(s) 66
BspT107I GGYRCC 1 cut(s) 154
BsrDI GCAATG 2 cut(s) 190, 450
BsrI ACTGG 2 cut(s) 55, 615
BssMI GATC 2 cut(s) 71, 342
Bst2UI CCWGG 1 cut(s) 548
Bst4CI ACNGT 1 cut(s) 636
Bst6I CTCTTC 3 cut(s) 222, 466, 786
BstDEI CTNAG 2 cut(s) 467, 534
BstEII GGTNACC 1 cut(s) 23
BstF5I GGATG 1 cut(s) 556
BstKTI GATC 2 cut(s) 74, 345
BstMAI GTCTC 2 cut(s) 719, 768
BstMBI GATC 2 cut(s) 71, 342
BstMWI GCNNNNNNNGC 1 cut(s) 784
BstNI CCWGG 1 cut(s) 548
BstPAI GACNNNNGTC 1 cut(s) 637
BstPI GGTNACC 1 cut(s) 23
BstSCI CCNGG 1 cut(s) 546
BstX2I RGATCY 1 cut(s) 71
BstYI RGATCY 1 cut(s) 71
BsuRI GGCC 1 cut(s) 455
BtsCI GGATG 1 cut(s) 556
BtsIMutI CAGTG 2 cut(s) 622, 641
Cfr13I GGNCC 2 cut(s) 398, 505
CspCI CAANNNNNGTGG 2 cut(s) 663, 698
CviAII CATG 1 cut(s) 593
CviJI RGCY 8 cut(s) 266, 301, 322, 352, 455, 488, 520, 778
CviKI_1 RGCY 8 cut(s) 266, 301, 322, 352, 455, 488, 520, 778
DdeI CTNAG 2 cut(s) 467, 534
DpnI GATC 2 cut(s) 73, 344
DpnII GATC 2 cut(s) 71, 342
DraI TTTAAA 1 cut(s) 211
DraIII CACNNNGTG 1 cut(s) 109
EaeI YGGCCR 1 cut(s) 453
Eam1104I CTCTTC 3 cut(s) 222, 466, 786
EarI CTCTTC 3 cut(s) 222, 466, 786
Eco47I GGWCC 2 cut(s) 398, 505
Eco88I CYCGRG 1 cut(s) 338
Eco91I GGTNACC 1 cut(s) 23
EcoO65I GGTNACC 1 cut(s) 23
EcoRII CCWGG 1 cut(s) 546
Esp3I CGTCTC 1 cut(s) 719
FaeI CATG 1 cut(s) 596
FatI CATG 1 cut(s) 592
FokI GGATG 1 cut(s) 563
FspBI CTAG 2 cut(s) 237, 820
HaeIII GGCC 1 cut(s) 455
HapII CCGG 2 cut(s) 75, 503
Hin1II CATG 1 cut(s) 596
HinfI GANTC 4 cut(s) 177, 270, 659, 794
HpaII CCGG 2 cut(s) 75, 503
HphI GGTGA 2 cut(s) 35, 95
Hpy166II GTNNAC 2 cut(s) 398, 421
Hpy188I TCNGA 3 cut(s) 48, 470, 606
Hpy188III TCNNGA 7 cut(s) 161, 305, 338, 340, 503, 757, 798
Hpy8I GTNNAC 2 cut(s) 398, 421
HpyAV CCTTC 2 cut(s) 505, 707
HpyCH4III ACNGT 1 cut(s) 636
HpyCH4IV ACGT 1 cut(s) 136
HpyCH4V TGCA 1 cut(s) 362
HpyF10VI GCNNNNNNNGC 1 cut(s) 784
HpyF3I CTNAG 2 cut(s) 467, 534
HpySE526I ACGT 1 cut(s) 136
Hsp92II CATG 1 cut(s) 596
Kpn2I TCCGGA 1 cut(s) 502
Kzo9I GATC 2 cut(s) 71, 342
LmnI GCTCC 1 cut(s) 4
LweI GCATC 2 cut(s) 225, 252
MaeI CTAG 2 cut(s) 237, 820
MaeII ACGT 1 cut(s) 136
MaeIII GTNAC 1 cut(s) 23
MalI GATC 2 cut(s) 73, 344
MboI GATC 2 cut(s) 71, 342
MboII GAAGA 6 cut(s) 239, 307, 383, 386, 483, 803
MfeI CAATTG 1 cut(s) 609
MflI RGATCY 1 cut(s) 71
MlsI TGGCCA 1 cut(s) 455
MluCI AATT 3 cut(s) 90, 355, 609
MluNI TGGCCA 1 cut(s) 455
MlyI GAGTC 1 cut(s) 803
Mox20I TGGCCA 1 cut(s) 455
MroI TCCGGA 1 cut(s) 502
MscI TGGCCA 1 cut(s) 455
MseI TTAA 3 cut(s) 87, 210, 597
Msp20I TGGCCA 1 cut(s) 455
MspI CCGG 2 cut(s) 75, 503
MspR9I CCNGG 1 cut(s) 548
MunI CAATTG 1 cut(s) 609
MvaI CCWGG 1 cut(s) 548
MwoI GCNNNNNNNGC 1 cut(s) 784
NdeII GATC 2 cut(s) 71, 342
NlaIII CATG 1 cut(s) 596
NlaIV GGNNCC 1 cut(s) 156
NmuCI GTSAC 1 cut(s) 23
PaeR7I CTCGAG 1 cut(s) 338
PfeI GAWTC 3 cut(s) 177, 270, 659
PleI GAGTC 1 cut(s) 802
PpsI GAGTC 1 cut(s) 802
PshAI GACNNNNGTC 1 cut(s) 637
Psp1406I AACGTT 1 cut(s) 136
Psp6I CCWGG 1 cut(s) 546
PspEI GGTNACC 1 cut(s) 23
PspGI CCWGG 1 cut(s) 546
PspN4I GGNNCC 1 cut(s) 156
PspPI GGNCC 2 cut(s) 398, 505
PsuI RGATCY 1 cut(s) 71
SaqAI TTAA 3 cut(s) 87, 210, 597
Sau3AI GATC 2 cut(s) 71, 342
Sau96I GGNCC 2 cut(s) 398, 505
SchI GAGTC 1 cut(s) 803
ScrFI CCNGG 1 cut(s) 548
SfaNI GCATC 2 cut(s) 225, 252
Sfr274I CTCGAG 1 cut(s) 338
SinI GGWCC 2 cut(s) 398, 505
SlaI CTCGAG 1 cut(s) 338
SmlI CTYRAG 2 cut(s) 161, 338
SmoI CTYRAG 2 cut(s) 161, 338
SpeI ACTAGT 1 cut(s) 236
Sse9I AATT 3 cut(s) 90, 355, 609
SspI AATATT 1 cut(s) 367
SspMI CTAG 2 cut(s) 237, 820
StyD4I CCNGG 1 cut(s) 546
TaaI ACNGT 1 cut(s) 636
TaiI ACGT 1 cut(s) 139
TaqI TCGA 5 cut(s) 132, 219, 225, 339, 345
TasI AATT 3 cut(s) 90, 355, 609
TfiI GAWTC 3 cut(s) 177, 270, 659
Tru1I TTAA 3 cut(s) 87, 210, 597
Tru9I TTAA 3 cut(s) 87, 210, 597
TscAI CASTG 2 cut(s) 622, 641
TseFI GTSAC 1 cut(s) 23
Tsp45I GTSAC 1 cut(s) 23
TspDTI ATGAA 5 cut(s) 129, 133, 280, 301, 725
TspGWI ACGGA 1 cut(s) 743
TspRI CASTG 2 cut(s) 622, 641
VpaK11BI GGWCC 2 cut(s) 398, 505
XhoI CTCGAG 1 cut(s) 338
XspI CTAG 2 cut(s) 237, 820
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.