Rh4CG027100

Belongs to the eukaryotic ribosomal protein eS12 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0004882
Physical Location & Seq
Forward (+)
5214598 .. 5226183
11586 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG027100.1

Sequence Viewer

Length: 1167 bp
ATGGCATTGGCCAATGGTCTGCTCAGAAGAGGACTTCCCGCAGCTTCCAATCTCATTCCTGACCAGAAGGTATGGCTGTGTGTTTTTGCTGAGGACAGCAACCAGGATGATTTTGTCAAGGCGGTAAAGTCATCGTGTGAGGGGTTTCATGTTAAGTATCTGACAATTGCCAGTGCTAAAACTCTTGGACAGTGGTCTCTGAAGAGAGGAAACAAAGCTGTGGTTGAGGATCTGAAAGATGAGACTGTGAAGATTGAAAAGAAAATTGAGCCTCCTAAGGCTGAAGAGAAAGATGAGGGGACTCCGATGGTTGAAGAGAAAGATGAGCCTGATAAGGTTGATGAGCAGACTCTGATGGTTGAAGAGAAAGATGGCTCTCATGGTGATATGGAGGAGACAGTGCAGGGACAGAACCAGACTGTGCCGTTTCAAGGTGTCGGTGGATATAGTGGCTGGCTGGAATACAATTACGATCAATCCAAGGGGTTTTATGATGGGGAGCTCGGCTTGAATGATGGAGAAAGAAAAGTGCCTGTTTTCTTTATCCCCTTCCAAGACTTGGAAACAGCAAGGAATAGACAAATTCGGGAGCTAGGAGAGGCTGTTGTGCAGATGCAAATGTCTAATGCTACTTATAGTGCACTCCATGAGAGCTCTAGTTTTGTTTTATGCAATGATGAAGTGAAATTAGTGAAGATTGATGGAAATTTGGAGAACGTGGAAGAGAATATTCGGTATCGGAGATTGGTGCAAGATTTTAAGAGATTTGGACAGACCGTTCGTCAGTACTTTCTTGATCCAAGAATCATAGATTGGCAACGAAGGGACGAGTTTTTGAAAATCTTCAACTTTGCAAATATAGGGTATGATAGGCTGACCAAGATGGTGAACAGGCACCCATTTTTAATGGTAAGTGATCATTGCCGGATGAAATATTTCTCGGAGATTTGGCATACGTTATCTAGTACTACTCTCAGTCAGAGGGTGTTGTATGCCTTGGGTTCAAGAGGTCTTATGCACTCCACTTATGTTCAGCAAAAAACCCATGGATGGTTTAGACCGGGCATGCGGAAGGAGTTCCATGCCATATTTGATTTCGAAGGCAGAGACAAGGACAAGGGCAGAGAGGCAATAGCTATGTTGGAACCAAATCAGACCAACTACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L7Ae

Protein Analysis

388

Amino Acids

44.83

Weight (kDa)

5.63

Isoelectric Point (pI)

41.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L7Ae PF01248 4 - 70 2.9e-06 Ribosomal protein L7Ae/L30e/S12e/Gadd45 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 894
AccB7I CCANNNNNTGG 1 cut(s) 559
AciI CCGC 3 cut(s) 39, 122, 1069
AclWI GGATC 2 cut(s) 237, 791
AcoI YGGCCR 1 cut(s) 9
AcsI RAATTY 2 cut(s) 582, 706
AcuI CTGAAG 2 cut(s) 221, 303
AfaI GTAC 2 cut(s) 788, 967
AfiI CCNNNNNNNGG 3 cut(s) 431, 559, 1050
AgsI TTSAA 8 cut(s) 257, 314, 362, 431, 511, 838, 847, 1005
AhdI GACNNNNNGTC 1 cut(s) 780
AjnI CCWGG 1 cut(s) 102
AjuI GAANNNNNNNTTGG 2 cut(s) 796, 828
AloI GAACNNNNNNTCC 2 cut(s) 762, 794
AluBI AGCT 6 cut(s) 44, 218, 502, 592, 654, 1136
AluI AGCT 6 cut(s) 44, 218, 502, 592, 654, 1136
Alw21I GWGCWC 3 cut(s) 504, 643, 656
Alw26I GTCTC 4 cut(s) 201, 236, 389, 1101
Alw44I GTGCAC 1 cut(s) 639
AlwI GGATC 2 cut(s) 237, 791
AlwNI CAGNNNCTG 1 cut(s) 352
AoxI GGCC 1 cut(s) 9
ApaLI GTGCAC 1 cut(s) 639
ApeKI GCWGC 1 cut(s) 41
ApoI RAATTY 2 cut(s) 582, 706
ArsI GACNNNNNNTTYG 2 cut(s) 762, 794
Asp700I GAANNNNTTC 3 cut(s) 842, 935, 1076
AsuC2I CCSGG 1 cut(s) 1062
AsuHPI GGTGA 2 cut(s) 395, 898
AsuII TTCGAA 1 cut(s) 1098
AxyI CCTNAGG 1 cut(s) 276
BaeGI GKGCMC 1 cut(s) 643
BalI TGGCCA 1 cut(s) 11
BanI GGYRCC 1 cut(s) 894
BanII GRGCYC 2 cut(s) 504, 656
Bbv12I GWGCWC 3 cut(s) 504, 643, 656
BbvCI CCTCAGC 1 cut(s) 90
BbvI GCAGC 1 cut(s) 53
BccI CCATC 8 cut(s) 301, 349, 365, 488, 509, 695, 877, 1044
BceAI ACGGC 1 cut(s) 409
BciT130I CCWGG 1 cut(s) 104
BclI TGATCA 1 cut(s) 916
BcnI CCSGG 1 cut(s) 1062
BcoDI GTCTC 4 cut(s) 201, 236, 389, 1101
BfaI CTAG 3 cut(s) 593, 657, 963
BisI GCNGC 1 cut(s) 42
BlsI GCNGC 1 cut(s) 43
BmcAI AGTACT 2 cut(s) 788, 967
Bme1390I CCNGG 2 cut(s) 104, 1062
BmeRI GACNNNNNGTC 1 cut(s) 780
BmiI GGNNCC 2 cut(s) 896, 1146
BmrFI CCNGG 2 cut(s) 104, 1062
BmsI GCATC 1 cut(s) 603
BoxI GACNNNNGTC 1 cut(s) 193
Bpu10I CCTNAGC 1 cut(s) 90
Bpu14I TTCGAA 1 cut(s) 1098
BpuMI CCSGG 1 cut(s) 1062
BsaI GGTCTC 1 cut(s) 201
BsaJI CCNNGG 3 cut(s) 480, 996, 1045
BsaXI ACNNNNNCTCC 2 cut(s) 588, 618
Bsc4I CCNNNNNNNGG 3 cut(s) 431, 559, 1050
Bse1I ACTGG 1 cut(s) 171
Bse21I CCTNAGG 1 cut(s) 276
Bse3DI GCAATG 2 cut(s) 679, 919
BseBI CCWGG 1 cut(s) 104
BseDI CCNNGG 3 cut(s) 480, 996, 1045
BseGI GGATG 3 cut(s) 112, 933, 1055
BseLI CCNNNNNNNGG 3 cut(s) 431, 559, 1050
BseMI GCAATG 2 cut(s) 679, 919
BseMII CTCAG 3 cut(s) 37, 81, 988
BseNI ACTGG 1 cut(s) 171
BseRI GAGGAG 1 cut(s) 407
BseSI GKGCMC 1 cut(s) 643
BseXI GCAGC 1 cut(s) 53
BsgI GTGCAG 2 cut(s) 422, 629
BshFI GGCC 1 cut(s) 11
BshNI GGYRCC 1 cut(s) 894
BsiHKAI GWGCWC 3 cut(s) 504, 643, 656
BsiSI CCGG 2 cut(s) 925, 1061
BslFI GGGAC 3 cut(s) 313, 420, 839
BslI CCNNNNNNNGG 3 cut(s) 431, 559, 1050
BsmAI GTCTC 4 cut(s) 201, 236, 389, 1101
BsmFI GGGAC 3 cut(s) 313, 420, 839
BsnI GGCC 1 cut(s) 11
Bso31I GGTCTC 1 cut(s) 201
Bsp119I TTCGAA 1 cut(s) 1098
Bsp1286I GDGCHC 3 cut(s) 504, 643, 656
Bsp143I GATC 4 cut(s) 229, 472, 796, 916
Bsp19I CCATGG 1 cut(s) 1045
BspACI CCGC 3 cut(s) 39, 122, 1069
BspANI GGCC 1 cut(s) 11
BspCNI CTCAG 3 cut(s) 36, 82, 987
BspLI GGNNCC 2 cut(s) 896, 1146
BspPI GGATC 2 cut(s) 237, 791
BspT104I TTCGAA 1 cut(s) 1098
BspT107I GGYRCC 1 cut(s) 894
BspTNI GGTCTC 1 cut(s) 201
BsrDI GCAATG 2 cut(s) 679, 919
BsrI ACTGG 1 cut(s) 171
BssECI CCNNGG 3 cut(s) 480, 996, 1045
BssMI GATC 4 cut(s) 229, 472, 796, 916
BssT1I CCWWGG 3 cut(s) 480, 996, 1045
Bst2UI CCWGG 1 cut(s) 104
Bst4CI ACNGT 5 cut(s) 192, 247, 400, 421, 778
Bst6I CTCTTC 6 cut(s) 22, 197, 279, 309, 357, 717
BstBI TTCGAA 1 cut(s) 1098
BstC8I GCNNGC 2 cut(s) 455, 1067
BstDEI CTNAG 4 cut(s) 23, 90, 276, 974
BstDSI CCRYGG 1 cut(s) 1045
BstF5I GGATG 3 cut(s) 112, 933, 1055
BstKTI GATC 4 cut(s) 232, 475, 799, 919
BstMAI GTCTC 4 cut(s) 201, 236, 389, 1101
BstMBI GATC 4 cut(s) 229, 472, 796, 916
BstNI CCWGG 1 cut(s) 104
BstNSI RCATGY 1 cut(s) 1069
BstPAI GACNNNNGTC 1 cut(s) 193
BstSCI CCNGG 2 cut(s) 102, 1060
BstSLI GKGCMC 1 cut(s) 643
BstV1I GCAGC 1 cut(s) 53
BstX2I RGATCY 1 cut(s) 229
BstYI RGATCY 1 cut(s) 229
Bsu36I CCTNAGG 1 cut(s) 276
BsuRI GGCC 1 cut(s) 11
BtgI CCRYGG 1 cut(s) 1045
BtsCI GGATG 3 cut(s) 112, 933, 1055
BtsIMutI CAGTG 3 cut(s) 178, 197, 405
Cac8I GCNNGC 2 cut(s) 455, 1067
CaiI CAGNNNCTG 1 cut(s) 352
Csp6I GTAC 2 cut(s) 787, 966
CviAII CATG 6 cut(s) 149, 380, 647, 1046, 1066, 1082
CviQI GTAC 2 cut(s) 787, 966
DdeI CTNAG 4 cut(s) 23, 90, 276, 974
DpnI GATC 4 cut(s) 231, 474, 798, 918
DpnII GATC 4 cut(s) 229, 472, 796, 916
DriI GACNNNNNGTC 1 cut(s) 780
EaeI YGGCCR 1 cut(s) 9
Eam1104I CTCTTC 6 cut(s) 22, 197, 279, 309, 357, 717
Eam1105I GACNNNNNGTC 1 cut(s) 780
EarI CTCTTC 6 cut(s) 22, 197, 279, 309, 357, 717
Ecl136II GAGCTC 2 cut(s) 502, 654
Eco130I CCWWGG 3 cut(s) 480, 996, 1045
Eco24I GRGCYC 2 cut(s) 504, 656
Eco31I GGTCTC 1 cut(s) 201
Eco53kI GAGCTC 2 cut(s) 502, 654
Eco57I CTGAAG 2 cut(s) 221, 303
Eco81I CCTNAGG 1 cut(s) 276
EcoICRI GAGCTC 2 cut(s) 502, 654
EcoRII CCWGG 1 cut(s) 102
EcoT14I CCWWGG 3 cut(s) 480, 996, 1045
EcoT38I GRGCYC 2 cut(s) 504, 656
ErhI CCWWGG 3 cut(s) 480, 996, 1045
FaeI CATG 6 cut(s) 152, 383, 650, 1049, 1069, 1085
FaqI GGGAC 3 cut(s) 313, 420, 839
FatI CATG 6 cut(s) 148, 379, 646, 1045, 1065, 1081
FauI CCCGC 1 cut(s) 46
FbaI TGATCA 1 cut(s) 916
Fnu4HI GCNGC 1 cut(s) 42
FokI GGATG 3 cut(s) 119, 940, 1062
FriOI GRGCYC 2 cut(s) 504, 656
Fsp4HI GCNGC 1 cut(s) 42
FspBI CTAG 3 cut(s) 593, 657, 963
GluI GCNGC 1 cut(s) 42
HaeIII GGCC 1 cut(s) 11
HapII CCGG 2 cut(s) 925, 1061
Hin1II CATG 6 cut(s) 152, 383, 650, 1049, 1069, 1085
HinfI GANTC 3 cut(s) 301, 349, 804
HpaII CCGG 2 cut(s) 925, 1061
HphI GGTGA 2 cut(s) 395, 898
Hpy166II GTNNAC 2 cut(s) 641, 889
Hpy188III TCNNGA 4 cut(s) 59, 587, 794, 1005
Hpy8I GTNNAC 2 cut(s) 641, 889
HpyAV CCTTC 5 cut(s) 61, 559, 816, 1066, 1094
HpyCH4III ACNGT 5 cut(s) 192, 247, 400, 421, 778
HpyCH4IV ACGT 2 cut(s) 717, 956
HpyCH4V TGCA 8 cut(s) 403, 610, 616, 641, 672, 751, 854, 1018
HpyF3I CTNAG 4 cut(s) 23, 90, 276, 974
HpySE526I ACGT 2 cut(s) 717, 956
Hsp92II CATG 6 cut(s) 152, 383, 650, 1049, 1069, 1085
Ksp22I TGATCA 1 cut(s) 916
Kzo9I GATC 4 cut(s) 229, 472, 796, 916
LmnI GCTCC 2 cut(s) 499, 589
Lsp1109I GCAGC 1 cut(s) 53
LweI GCATC 1 cut(s) 603
MaeI CTAG 3 cut(s) 593, 657, 963
MaeII ACGT 2 cut(s) 717, 956
MalI GATC 4 cut(s) 231, 474, 798, 918
MboI GATC 4 cut(s) 229, 472, 796, 916
MboII GAAGA 9 cut(s) 39, 214, 262, 296, 326, 374, 706, 734, 835
MfeI CAATTG 1 cut(s) 165
MflI RGATCY 1 cut(s) 229
MhlI GDGCHC 3 cut(s) 504, 643, 656
MlsI TGGCCA 1 cut(s) 11
MluCI AATT 6 cut(s) 165, 264, 466, 582, 686, 706
MluNI TGGCCA 1 cut(s) 11
MlyI GAGTC 2 cut(s) 295, 343
MmeI TCCRAC 1 cut(s) 1122
Mox20I TGGCCA 1 cut(s) 11
MroXI GAANNNNTTC 3 cut(s) 842, 935, 1076
MscI TGGCCA 1 cut(s) 11
MseI TTAA 3 cut(s) 153, 759, 905
Msp20I TGGCCA 1 cut(s) 11
MspI CCGG 2 cut(s) 925, 1061
MspR9I CCNGG 2 cut(s) 104, 1062
MunI CAATTG 1 cut(s) 165
MvaI CCWGG 1 cut(s) 104
NciI CCSGG 1 cut(s) 1062
NcoI CCATGG 1 cut(s) 1045
NdeII GATC 4 cut(s) 229, 472, 796, 916
NlaIII CATG 6 cut(s) 152, 383, 650, 1049, 1069, 1085
NlaIV GGNNCC 2 cut(s) 896, 1146
NmeAIII GCCGAG 1 cut(s) 483
NspI RCATGY 1 cut(s) 1069
NspV TTCGAA 1 cut(s) 1098
PaeI GCATGC 1 cut(s) 1069
PdmI GAANNNNTTC 3 cut(s) 842, 935, 1076
PfeI GAWTC 1 cut(s) 804
PflMI CCANNNNNTGG 1 cut(s) 559
PkrI GCNGC 1 cut(s) 43
PleI GAGTC 2 cut(s) 295, 343
PpsI GAGTC 2 cut(s) 295, 343
PshAI GACNNNNGTC 1 cut(s) 193
Psp124BI GAGCTC 2 cut(s) 504, 656
Psp6I CCWGG 1 cut(s) 102
PspGI CCWGG 1 cut(s) 102
PspN4I GGNNCC 2 cut(s) 896, 1146
PstNI CAGNNNCTG 1 cut(s) 352
PsuI RGATCY 1 cut(s) 229
RsaI GTAC 2 cut(s) 788, 967
RsaNI GTAC 2 cut(s) 787, 966
SacI GAGCTC 2 cut(s) 504, 656
SaqAI TTAA 3 cut(s) 153, 759, 905
SatI GCNGC 1 cut(s) 42
Sau3AI GATC 4 cut(s) 229, 472, 796, 916
ScaI AGTACT 2 cut(s) 788, 967
SchI GAGTC 2 cut(s) 295, 343
ScrFI CCNGG 2 cut(s) 104, 1062
SduI GDGCHC 3 cut(s) 504, 643, 656
SfaNI GCATC 1 cut(s) 603
SfuI TTCGAA 1 cut(s) 1098
SphI GCATGC 1 cut(s) 1069
Sse9I AATT 6 cut(s) 165, 264, 466, 582, 686, 706
SsiI CCGC 3 cut(s) 39, 122, 1069
SspI AATATT 2 cut(s) 730, 935
SspMI CTAG 3 cut(s) 593, 657, 963
SstI GAGCTC 2 cut(s) 504, 656
StyD4I CCNGG 2 cut(s) 102, 1060
StyI CCWWGG 3 cut(s) 480, 996, 1045
TaaI ACNGT 5 cut(s) 192, 247, 400, 421, 778
TaiI ACGT 2 cut(s) 720, 959
TaqI TCGA 1 cut(s) 1098
TasI AATT 6 cut(s) 165, 264, 466, 582, 686, 706
TatI WGTACW 2 cut(s) 786, 965
TfiI GAWTC 1 cut(s) 804
Tru1I TTAA 3 cut(s) 153, 759, 905
Tru9I TTAA 3 cut(s) 153, 759, 905
TscAI CASTG 3 cut(s) 178, 197, 405
TseI GCWGC 1 cut(s) 41
TspDTI ATGAA 3 cut(s) 137, 693, 944
TspRI CASTG 3 cut(s) 178, 197, 405
Van91I CCANNNNNTGG 1 cut(s) 559
VneI GTGCAC 1 cut(s) 639
XapI RAATTY 2 cut(s) 582, 706
XceI RCATGY 1 cut(s) 1069
XmnI GAANNNNTTC 3 cut(s) 842, 935, 1076
XspI CTAG 3 cut(s) 593, 657, 963
ZrmI AGTACT 2 cut(s) 788, 967
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.