Rh7BG028700

40S ribosomal protein S12-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7B
OG0004882
Physical Location & Seq
Reverse (-)
1968087 .. 1975681
7595 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7BG028700.1

Sequence Viewer

Length: 645 bp
ATGTTTCTGATTGTTTTATGTCTCAGTACTTTTAAACGCATTGAACCAGAAGAGATAGCTAGTCGCATCAACCTCTTAAACCAAGAAGACAGATACGGCATTGATACTGACTTCATCATCAAGCCTGTTGACTTCATCTTCAAGCCAGAATATGATTATCTCGAGATCGACCAGCCAAATTATGCAAATATTGAAGAAGAAAATATGTCCTTACAAGGTGGACCTCATTTCAAAAACTTCGTGGACTCATTGAGAGATTTGTTGGCAATGGCATTGGCCAATGGTTTGCTCAGAAGAGGACTTCCCGCAGCTTCCAATTTCATGCCGGACCAGAAGGTATGGCTGTGTGTTTTTGCTGAGGACAGCAACCAGGATGATTTTGTCAAGGCGGTAAAGTCATTGTGTGAGGAGTATCATGTTAAGTATCTGACAATTGCCAGTGCTAAAACTCTTGGACAGTGGTCTGTGTTTGGTAATAAGGATTCTGAAGGAAAGGCAAAGAGGGTCGTTCGTTGCTCTTGCGTTGTTATTGAGGAAGGCATATATGAGACGGAACTTCATAAAGCAGTTTTGGAGGTCTGGAAGTCTCTCCCATCAAAGCTGGAAGTGCTGGAAGAGTCTGGATTGTGTCGCCCTTTTGTCTAG

Protein Analysis

214

Amino Acids

24.45

Weight (kDa)

4.89

Isoelectric Point (pI)

55.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L7Ae PF01248 84 - 175 2.4e-09 Ribosomal protein L7Ae/L30e/S12e/Gadd45 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 306, 389
AcoI YGGCCR 1 cut(s) 276
AcuI CTGAAG 1 cut(s) 507
AfaI GTAC 1 cut(s) 28
AgsI TTSAA 4 cut(s) 44, 142, 194, 232
AjnI CCWGG 1 cut(s) 369
AluBI AGCT 3 cut(s) 59, 311, 601
AluI AGCT 3 cut(s) 59, 311, 601
Alw26I GTCTC 3 cut(s) 26, 542, 591
Ama87I CYCGRG 1 cut(s) 161
AoxI GGCC 1 cut(s) 276
ApeKI GCWGC 1 cut(s) 308
AspS9I GGNCC 2 cut(s) 221, 328
AvaI CYCGRG 1 cut(s) 161
AvaII GGWCC 2 cut(s) 221, 328
BalI TGGCCA 1 cut(s) 278
BbsI GAAGAC 1 cut(s) 93
BbvCI CCTCAGC 1 cut(s) 357
BbvI GCAGC 1 cut(s) 320
BccI CCATC 1 cut(s) 601
BceAI ACGGC 1 cut(s) 112
BciT130I CCWGG 1 cut(s) 371
BcoDI GTCTC 3 cut(s) 26, 542, 591
BfaI CTAG 2 cut(s) 60, 643
BisI GCNGC 1 cut(s) 309
BlsI GCNGC 1 cut(s) 310
BmcAI AGTACT 1 cut(s) 28
Bme1390I CCNGG 1 cut(s) 371
Bme18I GGWCC 2 cut(s) 221, 328
BmeT110I CYCGRG 1 cut(s) 161
BmgT120I GGNCC 2 cut(s) 221, 328
BmrFI CCNGG 1 cut(s) 371
BmsI GCATC 1 cut(s) 75
BoxI GACNNNNGTC 1 cut(s) 460
BpiI GAAGAC 1 cut(s) 93
Bpu10I CCTNAGC 1 cut(s) 357
Bse1I ACTGG 1 cut(s) 438
Bse3DI GCAATG 1 cut(s) 273
BseBI CCWGG 1 cut(s) 371
BseGI GGATG 1 cut(s) 379
BseMI GCAATG 1 cut(s) 273
BseMII CTCAG 3 cut(s) 37, 304, 348
BseNI ACTGG 1 cut(s) 438
BseRI GAGGAG 1 cut(s) 422
BseXI GCAGC 1 cut(s) 320
BshFI GGCC 1 cut(s) 278
BsiHKCI CYCGRG 1 cut(s) 161
BsiSI CCGG 1 cut(s) 326
BsmAI GTCTC 3 cut(s) 26, 542, 591
BsmBI CGTCTC 1 cut(s) 542
BsnI GGCC 1 cut(s) 278
BsoBI CYCGRG 1 cut(s) 161
Bsp143I GATC 1 cut(s) 165
BspACI CCGC 2 cut(s) 306, 389
BspANI GGCC 1 cut(s) 278
BspCNI CTCAG 3 cut(s) 36, 303, 349
BsrDI GCAATG 1 cut(s) 273
BsrI ACTGG 1 cut(s) 438
BssMI GATC 1 cut(s) 165
Bst2UI CCWGG 1 cut(s) 371
Bst4CI ACNGT 1 cut(s) 459
Bst6I CTCTTC 3 cut(s) 45, 289, 609
BstDEI CTNAG 3 cut(s) 23, 290, 357
BstF5I GGATG 1 cut(s) 379
BstKTI GATC 1 cut(s) 168
BstMAI GTCTC 3 cut(s) 26, 542, 591
BstMBI GATC 1 cut(s) 165
BstMWI GCNNNNNNNGC 1 cut(s) 607
BstNI CCWGG 1 cut(s) 371
BstPAI GACNNNNGTC 1 cut(s) 460
BstSCI CCNGG 1 cut(s) 369
BstV1I GCAGC 1 cut(s) 320
BstV2I GAAGAC 1 cut(s) 93
BsuRI GGCC 1 cut(s) 278
BtsCI GGATG 1 cut(s) 379
BtsIMutI CAGTG 2 cut(s) 445, 464
Cfr13I GGNCC 2 cut(s) 221, 328
Csp6I GTAC 1 cut(s) 27
CviAII CATG 2 cut(s) 322, 416
CviJI RGCY 8 cut(s) 59, 124, 145, 175, 278, 311, 343, 601
CviKI_1 RGCY 8 cut(s) 59, 124, 145, 175, 278, 311, 343, 601
CviQI GTAC 1 cut(s) 27
DdeI CTNAG 3 cut(s) 23, 290, 357
DpnI GATC 1 cut(s) 167
DpnII GATC 1 cut(s) 165
DraI TTTAAA 1 cut(s) 34
EaeI YGGCCR 1 cut(s) 276
Eam1104I CTCTTC 3 cut(s) 45, 289, 609
EarI CTCTTC 3 cut(s) 45, 289, 609
Eco47I GGWCC 2 cut(s) 221, 328
Eco57I CTGAAG 1 cut(s) 507
Eco88I CYCGRG 1 cut(s) 161
EcoRII CCWGG 1 cut(s) 369
Esp3I CGTCTC 1 cut(s) 542
FaeI CATG 2 cut(s) 325, 419
FatI CATG 2 cut(s) 321, 415
FauI CCCGC 1 cut(s) 313
Fnu4HI GCNGC 1 cut(s) 309
FokI GGATG 1 cut(s) 386
Fsp4HI GCNGC 1 cut(s) 309
FspBI CTAG 2 cut(s) 60, 643
GluI GCNGC 1 cut(s) 309
HaeIII GGCC 1 cut(s) 278
HapII CCGG 1 cut(s) 326
Hin1II CATG 2 cut(s) 325, 419
HincII GTYRAC 1 cut(s) 130
HindII GTYRAC 1 cut(s) 130
HinfI GANTC 3 cut(s) 245, 482, 617
HpaII CCGG 1 cut(s) 326
Hpy166II GTNNAC 3 cut(s) 130, 221, 244
Hpy188I TCNGA 4 cut(s) 9, 293, 429, 487
Hpy188III TCNNGA 4 cut(s) 161, 163, 580, 621
Hpy8I GTNNAC 3 cut(s) 130, 221, 244
HpyAV CCTTC 3 cut(s) 328, 482, 530
HpyCH4III ACNGT 1 cut(s) 459
HpyCH4V TGCA 1 cut(s) 185
HpyF10VI GCNNNNNNNGC 1 cut(s) 607
HpyF3I CTNAG 3 cut(s) 23, 290, 357
Hsp92II CATG 2 cut(s) 325, 419
Kzo9I GATC 1 cut(s) 165
Lsp1109I GCAGC 1 cut(s) 320
LweI GCATC 1 cut(s) 75
MaeI CTAG 2 cut(s) 60, 643
MalI GATC 1 cut(s) 167
MboI GATC 1 cut(s) 165
MboII GAAGA 7 cut(s) 62, 98, 130, 206, 209, 306, 626
MfeI CAATTG 1 cut(s) 432
MlsI TGGCCA 1 cut(s) 278
MluCI AATT 3 cut(s) 178, 316, 432
MluNI TGGCCA 1 cut(s) 278
MlyI GAGTC 2 cut(s) 239, 626
MnlI CCTC 8 cut(s) 83, 234, 290, 352, 400, 495, 526, 568
Mox20I TGGCCA 1 cut(s) 278
MscI TGGCCA 1 cut(s) 278
MseI TTAA 3 cut(s) 33, 77, 420
Msp20I TGGCCA 1 cut(s) 278
MspI CCGG 1 cut(s) 326
MspR9I CCNGG 1 cut(s) 371
MunI CAATTG 1 cut(s) 432
MvaI CCWGG 1 cut(s) 371
MwoI GCNNNNNNNGC 1 cut(s) 607
NdeII GATC 1 cut(s) 165
NlaIII CATG 2 cut(s) 325, 419
PaeR7I CTCGAG 1 cut(s) 161
PfeI GAWTC 1 cut(s) 482
PkrI GCNGC 1 cut(s) 310
PleI GAGTC 2 cut(s) 239, 625
PpsI GAGTC 2 cut(s) 239, 625
PshAI GACNNNNGTC 1 cut(s) 460
Psp6I CCWGG 1 cut(s) 369
PspGI CCWGG 1 cut(s) 369
PspPI GGNCC 2 cut(s) 221, 328
RsaI GTAC 1 cut(s) 28
RsaNI GTAC 1 cut(s) 27
SaqAI TTAA 3 cut(s) 33, 77, 420
SatI GCNGC 1 cut(s) 309
Sau3AI GATC 1 cut(s) 165
Sau96I GGNCC 2 cut(s) 221, 328
ScaI AGTACT 1 cut(s) 28
SchI GAGTC 2 cut(s) 239, 626
ScrFI CCNGG 1 cut(s) 371
SetI ASST 8 cut(s) 61, 75, 220, 226, 313, 339, 579, 603
SfaNI GCATC 1 cut(s) 75
Sfr274I CTCGAG 1 cut(s) 161
SinI GGWCC 2 cut(s) 221, 328
SlaI CTCGAG 1 cut(s) 161
SmlI CTYRAG 1 cut(s) 161
SmoI CTYRAG 1 cut(s) 161
Sse9I AATT 3 cut(s) 178, 316, 432
SsiI CCGC 2 cut(s) 306, 389
SspI AATATT 1 cut(s) 190
SspMI CTAG 2 cut(s) 60, 643
StyD4I CCNGG 1 cut(s) 369
TaaI ACNGT 1 cut(s) 459
TaqI TCGA 2 cut(s) 162, 168
TasI AATT 3 cut(s) 178, 316, 432
TatI WGTACW 1 cut(s) 26
TfiI GAWTC 1 cut(s) 482
Tru1I TTAA 3 cut(s) 33, 77, 420
Tru9I TTAA 3 cut(s) 33, 77, 420
TscAI CASTG 2 cut(s) 445, 464
TseI GCWGC 1 cut(s) 308
TspDTI ATGAA 4 cut(s) 103, 124, 310, 548
TspGWI ACGGA 1 cut(s) 566
TspRI CASTG 2 cut(s) 445, 464
VpaK11BI GGWCC 2 cut(s) 221, 328
XhoI CTCGAG 1 cut(s) 161
XspI CTAG 2 cut(s) 60, 643
ZrmI AGTACT 1 cut(s) 28
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.