Rroxscaffold_5G00353800

40S ribosomal protein S12-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0004882
Physical Location & Seq
Forward (+)
31753535 .. 31755202
1668 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00353800.1

Sequence Viewer

Length: 1668 bp
ATGATAAACCAGGCAGAAGAAGAAGCAACGTTTCTTTATATCATGATGCAATATGAGGACGGCCCGCACTTTGGATCGTTGTATGAATTTAATTTATGTAAGCAAGACTCCTTTCGACCTTTAGCACAGTTCAGGGATGAGGATGTTAACCTTCCGTTAAGTTGGAAATTTTGCTCCTTGGATACCAACAACATATGTCTATTGCCGGATAGTGGTCCACCTCCTGATGATGTGGTGAACCCCTTGTCTGGATATATGTTCAATGTTGATACCAATAAATGTCATTTAATCAAGCCGCCCGTTGCTCCCAAATGGAACGGACCTGTTGTATCTGCATGTGGGAAACTTTACTCTATTACGGATCCATACTGCTCATTTGATGTGCCAGAGCCCTCTTTCGAGCGATATGATCCTTACAGGTGTTGTTGGGAAACTCGGAAATCTTTTCCTTATGTTCAAAATTGTTCCGAACAAAGGATTGGGGGTCATGCCGTTTGTGATGGACTCATCCTGTATAATATAATCCCTTCTGTTGGATTCATTCAGGAGGATAATCCATTCTCTTTCTCTAAAAAATATCAAGATGAACTGTGGGCTTATGATGTAACGCTGGATGAATGGCTTCCAGTTAGGGTCGAGAACACAGATTGCTATAGTTTCCGAGGACCGGCTGTATGTGTGGACAAGACTATCTATGCGTTATCAAGCAGCTTGGCGGTTATGGCACTCTCTATCACAAGGCATGAAAATCTTGTTACCTGCTCTCTAAAGTCACAGTATGTTTGTCCAGAGTTTGGGGACTTTCGACCAGGCGCACAGAGAAGGGAGCATTTGATTTATTTGGGTGACCTGAACTTTTGCCTTGTTCAGACTGGATTTCAGTATGGCGAAATTAGATCCAGTCAATCTCTTAAAATTACCACATTTCACCTTGTGGGAGAAACCAATATCAAAATCGAACGTTCATTCATTTATGAGGTGCCTCTTGAGGGTTGTATGAAATTCACAATTGGACATTGCTTTATCCATACTTTTAAACGCATTGAACTAGAAGAGGTGGGTAGTCGCATCGACTTCGTAAACCAAGAATACAGATTCGGCATTGAAACTGACTTCATCATGAAGCCTGTTGACTTCATCTTCAAGCCAGAATATGATTATCTCGAGATCAACCAGCCAAATTATGCAAATATTGAAGAAGAAAATATGTCCTTACAAGGTGGACCTCATTTCAAAAACTTCGTGGACTTGTTGAAAGATTTGTTGGCAATGGCATTGGCCAATGGTTTGCTCAGAAGAGGACTTCCCGCAGCTTCCAATCTCATTCCGTACCAGAAGGTATGGCTGTGTGTTTTTGCTGAGGACAGCAACCAGGATGATTTTGTCGAGGCGGTAAAGTCATCGTGTGAGGGGTATCATGTTAAGTATCTGACAATTGCCAGTGCTAAAACTCTTGGACAGTGGTCTCTGTCTGGTAATAAGGATTCTGAAGGAAAGGCAAAGAGGGTGGTTCATTGCTCTTGTGTTGTTATTGAGGAAGGCATATATGGGACGGAACTTCATAAAGCAGTTTTGGAAGTCTGTAAGTCTCTCCCATCAAAGCTGGAAGTGCTGGAAGAGTCTGGACAGTGTCACCCTGTTGTAGGGTCAAGGGCTTATTTAGAATAG

Protein Analysis

555

Amino Acids

63.15

Weight (kDa)

5.0

Isoelectric Point (pI)

47.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L7Ae PF01248 419 - 510 4.6e-07 Ribosomal protein L7Ae/L30e/S12e/Gadd45 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 767
AccB1I GGYRCC 1 cut(s) 979
AccB7I CCANNNNNTGG 1 cut(s) 794
AciI CCGC 5 cut(s) 65, 296, 716, 1308, 1391
AclI AACGTT 2 cut(s) 29, 961
AclWI GGATC 5 cut(s) 82, 356, 369, 404, 891
AcoI YGGCCR 1 cut(s) 1278
AcsI RAATTY 3 cut(s) 86, 167, 1001
AcuI CTGAAG 1 cut(s) 1509
AdeI CACNNNGTG 1 cut(s) 934
AfaI GTAC 1 cut(s) 1331
AfiI CCNNNNNNNGG 9 cut(s) 71, 212, 248, 474, 533, 667, 794, 989, 1643
AgsI TTSAA 8 cut(s) 262, 458, 1046, 1106, 1144, 1196, 1234, 1255
AjnI CCWGG 3 cut(s) 9, 808, 1371
AjuI GAANNNNNNNTTGG 4 cut(s) 462, 494, 1247, 1279
AluBI AGCT 3 cut(s) 711, 1313, 1603
AluI AGCT 3 cut(s) 711, 1313, 1603
Alw26I GTCTC 2 cut(s) 1470, 1593
AlwI GGATC 5 cut(s) 82, 356, 369, 404, 891
Ama87I CYCGRG 1 cut(s) 1163
AoxI GGCC 2 cut(s) 61, 1278
ApeKI GCWGC 2 cut(s) 708, 1310
ApoI RAATTY 3 cut(s) 86, 167, 1001
Asp700I GAANNNNTTC 1 cut(s) 621
AspLEI GCGC 1 cut(s) 815
AspS9I GGNCC 5 cut(s) 62, 215, 320, 665, 1223
AsuHPI GGTGA 4 cut(s) 247, 857, 920, 1625
AvaI CYCGRG 1 cut(s) 1163
AvaII GGWCC 4 cut(s) 215, 320, 665, 1223
BalI TGGCCA 1 cut(s) 1280
BamHI GGATCC 1 cut(s) 361
BanI GGYRCC 1 cut(s) 979
BanII GRGCYC 1 cut(s) 393
BbvCI CCTCAGC 1 cut(s) 1359
BbvI GCAGC 2 cut(s) 720, 1322
BccI CCATC 2 cut(s) 494, 1603
BceAI ACGGC 2 cut(s) 76, 476
BcgI CGANNNNNNTGC 2 cut(s) 1057, 1091
BciT130I CCWGG 3 cut(s) 11, 810, 1373
BciVI GTATCC 1 cut(s) 175
BcoDI GTCTC 2 cut(s) 1470, 1593
BfaI CTAG 1 cut(s) 1049
BfmI CTRYAG 1 cut(s) 652
BfuAI ACCTGC 1 cut(s) 767
BfuI GTATCC 1 cut(s) 175
BisI GCNGC 3 cut(s) 296, 709, 1311
BlsI GCNGC 3 cut(s) 297, 710, 1312
Bme1390I CCNGG 3 cut(s) 11, 810, 1373
Bme18I GGWCC 4 cut(s) 215, 320, 665, 1223
BmeT110I CYCGRG 1 cut(s) 1163
BmgT120I GGNCC 5 cut(s) 62, 215, 320, 665, 1223
BmiI GGNNCC 2 cut(s) 363, 981
BmrFI CCNGG 3 cut(s) 11, 810, 1373
BmsI GCATC 2 cut(s) 36, 1077
BoxI GACNNNNGTC 1 cut(s) 1462
Bpu10I CCTNAGC 1 cut(s) 1359
BpuEI CTTGAG 1 cut(s) 1007
BsaI GGTCTC 1 cut(s) 1470
BsaJI CCNNGG 2 cut(s) 177, 661
Bsc4I CCNNNNNNNGG 9 cut(s) 71, 212, 248, 474, 533, 667, 794, 989, 1643
Bse118I RCCGGY 1 cut(s) 667
Bse1I ACTGG 4 cut(s) 626, 877, 900, 1440
Bse3DI GCAATG 3 cut(s) 1015, 1275, 1513
BseBI CCWGG 3 cut(s) 11, 810, 1373
BseDI CCNNGG 2 cut(s) 177, 661
BseGI GGATG 5 cut(s) 142, 148, 507, 619, 1381
BseLI CCNNNNNNNGG 9 cut(s) 71, 212, 248, 474, 533, 667, 794, 989, 1643
BseMI GCAATG 3 cut(s) 1015, 1275, 1513
BseMII CTCAG 2 cut(s) 1306, 1350
BseNI ACTGG 4 cut(s) 626, 877, 900, 1440
BseXI GCAGC 2 cut(s) 720, 1322
BshFI GGCC 2 cut(s) 63, 1280
BshNI GGYRCC 1 cut(s) 979
BsiHKCI CYCGRG 1 cut(s) 1163
BsiSI CCGG 2 cut(s) 206, 668
BslFI GGGAC 2 cut(s) 812, 1564
BslI CCNNNNNNNGG 9 cut(s) 71, 212, 248, 474, 533, 667, 794, 989, 1643
BsmAI GTCTC 2 cut(s) 1470, 1593
BsmFI GGGAC 2 cut(s) 812, 1564
BsnI GGCC 2 cut(s) 63, 1280
Bso31I GGTCTC 1 cut(s) 1470
BsoBI CYCGRG 1 cut(s) 1163
Bsp1286I GDGCHC 1 cut(s) 393
Bsp143I GATC 5 cut(s) 74, 361, 409, 896, 1167
BspACI CCGC 5 cut(s) 65, 296, 716, 1308, 1391
BspANI GGCC 2 cut(s) 63, 1280
BspCNI CTCAG 2 cut(s) 1305, 1351
BspHI TCATGA 2 cut(s) 42, 1119
BspLI GGNNCC 2 cut(s) 363, 981
BspMI ACCTGC 1 cut(s) 767
BspPI GGATC 5 cut(s) 82, 356, 369, 404, 891
BspT107I GGYRCC 1 cut(s) 979
BspTNI GGTCTC 1 cut(s) 1470
BsrDI GCAATG 3 cut(s) 1015, 1275, 1513
BsrFI RCCGGY 1 cut(s) 667
BsrI ACTGG 4 cut(s) 626, 877, 900, 1440
BssAI RCCGGY 1 cut(s) 667
BssECI CCNNGG 2 cut(s) 177, 661
BssMI GATC 5 cut(s) 74, 361, 409, 896, 1167
BssT1I CCWWGG 1 cut(s) 177
Bst2UI CCWGG 3 cut(s) 11, 810, 1373
Bst4CI ACNGT 5 cut(s) 129, 591, 777, 1461, 1629
Bst6I CTCTTC 3 cut(s) 1047, 1291, 1611
BstC8I GCNNGC 1 cut(s) 65
BstDEI CTNAG 2 cut(s) 1292, 1359
BstEII GGTNACC 1 cut(s) 845
BstENI CCTNNNNNAGG 1 cut(s) 1641
BstF5I GGATG 5 cut(s) 142, 148, 507, 619, 1381
BstHHI GCGC 1 cut(s) 815
BstKTI GATC 5 cut(s) 77, 364, 412, 899, 1170
BstMAI GTCTC 2 cut(s) 1470, 1593
BstMBI GATC 5 cut(s) 74, 361, 409, 896, 1167
BstMWI GCNNNNNNNGC 2 cut(s) 722, 1609
BstNI CCWGG 3 cut(s) 11, 810, 1373
BstNSI RCATGY 1 cut(s) 339
BstPAI GACNNNNGTC 1 cut(s) 1462
BstPI GGTNACC 1 cut(s) 845
BstSCI CCNGG 3 cut(s) 9, 808, 1371
BstSFI CTRYAG 1 cut(s) 652
BstV1I GCAGC 2 cut(s) 720, 1322
BstX2I RGATCY 2 cut(s) 361, 896
BstYI RGATCY 2 cut(s) 361, 896
BsuI GTATCC 1 cut(s) 175
BsuRI GGCC 2 cut(s) 63, 1280
BtsCI GGATG 5 cut(s) 142, 148, 507, 619, 1381
BtsIMutI CAGTG 3 cut(s) 1447, 1466, 1634
BveI ACCTGC 1 cut(s) 767
Cac8I GCNNGC 1 cut(s) 65
CciI TCATGA 2 cut(s) 42, 1119
CfoI GCGC 1 cut(s) 815
Cfr10I RCCGGY 1 cut(s) 667
Cfr13I GGNCC 5 cut(s) 62, 215, 320, 665, 1223
Csp6I GTAC 1 cut(s) 1330
CspCI CAANNNNNGTGG 2 cut(s) 1488, 1523
CviAII CATG 6 cut(s) 43, 336, 488, 743, 1120, 1418
CviQI GTAC 1 cut(s) 1330
DdeI CTNAG 2 cut(s) 1292, 1359
DpnI GATC 5 cut(s) 76, 363, 411, 898, 1169
DpnII GATC 5 cut(s) 74, 361, 409, 896, 1167
DraI TTTAAA 1 cut(s) 1036
DraIII CACNNNGTG 1 cut(s) 934
EaeI YGGCCR 1 cut(s) 1278
Eam1104I CTCTTC 3 cut(s) 1047, 1291, 1611
EarI CTCTTC 3 cut(s) 1047, 1291, 1611
Eco130I CCWWGG 1 cut(s) 177
Eco24I GRGCYC 1 cut(s) 393
Eco31I GGTCTC 1 cut(s) 1470
Eco47I GGWCC 4 cut(s) 215, 320, 665, 1223
Eco57I CTGAAG 1 cut(s) 1509
Eco88I CYCGRG 1 cut(s) 1163
Eco91I GGTNACC 1 cut(s) 845
EcoNI CCTNNNNNAGG 1 cut(s) 1641
EcoO65I GGTNACC 1 cut(s) 845
EcoRII CCWGG 3 cut(s) 9, 808, 1371
EcoT14I CCWWGG 1 cut(s) 177
EcoT38I GRGCYC 1 cut(s) 393
ErhI CCWWGG 1 cut(s) 177
FaeI CATG 6 cut(s) 46, 339, 491, 746, 1123, 1421
FaqI GGGAC 2 cut(s) 812, 1564
FatI CATG 6 cut(s) 42, 335, 487, 742, 1119, 1417
FauI CCCGC 2 cut(s) 72, 1315
FauNDI CATATG 1 cut(s) 194
Fnu4HI GCNGC 3 cut(s) 296, 709, 1311
FokI GGATG 5 cut(s) 149, 155, 494, 626, 1388
FriOI GRGCYC 1 cut(s) 393
Fsp4HI GCNGC 3 cut(s) 296, 709, 1311
FspBI CTAG 1 cut(s) 1049
GlaI GCGC 1 cut(s) 814
GluI GCNGC 3 cut(s) 296, 709, 1311
HaeIII GGCC 2 cut(s) 63, 1280
HapII CCGG 2 cut(s) 206, 668
HhaI GCGC 1 cut(s) 815
Hin1II CATG 6 cut(s) 46, 339, 491, 746, 1123, 1421
Hin6I GCGC 1 cut(s) 813
HinP1I GCGC 1 cut(s) 813
HincII GTYRAC 2 cut(s) 148, 1132
HindII GTYRAC 2 cut(s) 148, 1132
HinfI GANTC 6 cut(s) 107, 504, 537, 1095, 1484, 1619
HpaI GTTAAC 1 cut(s) 148
HpaII CCGG 2 cut(s) 206, 668
HphI GGTGA 4 cut(s) 247, 857, 920, 1625
Hpy166II GTNNAC 8 cut(s) 148, 218, 238, 682, 1081, 1132, 1223, 1246
Hpy188I TCNGA 7 cut(s) 438, 469, 662, 870, 1295, 1431, 1489
Hpy8I GTNNAC 8 cut(s) 148, 218, 238, 682, 1081, 1132, 1223, 1246
HpyAV CCTTC 6 cut(s) 161, 537, 816, 1330, 1484, 1532
HpyCH4III ACNGT 5 cut(s) 129, 591, 777, 1461, 1629
HpyCH4IV ACGT 2 cut(s) 29, 961
HpyCH4V TGCA 3 cut(s) 49, 335, 1187
HpyF10VI GCNNNNNNNGC 2 cut(s) 722, 1609
HpyF3I CTNAG 2 cut(s) 1292, 1359
HpySE526I ACGT 2 cut(s) 29, 961
Hsp92II CATG 6 cut(s) 46, 339, 491, 746, 1123, 1421
HspAI GCGC 1 cut(s) 813
KspAI GTTAAC 1 cut(s) 148
Kzo9I GATC 5 cut(s) 74, 361, 409, 896, 1167
LmnI GCTCC 3 cut(s) 179, 310, 826
Lsp1109I GCAGC 2 cut(s) 720, 1322
LweI GCATC 2 cut(s) 36, 1077
MaeI CTAG 1 cut(s) 1049
MaeII ACGT 2 cut(s) 29, 961
MaeIII GTNAC 5 cut(s) 604, 754, 771, 845, 1631
MalI GATC 5 cut(s) 76, 363, 411, 898, 1169
MboI GATC 5 cut(s) 74, 361, 409, 896, 1167
MboII GAAGA 8 cut(s) 29, 32, 1064, 1132, 1208, 1211, 1308, 1628
MfeI CAATTG 2 cut(s) 1008, 1434
MflI RGATCY 2 cut(s) 361, 896
MhlI GDGCHC 1 cut(s) 393
MlsI TGGCCA 1 cut(s) 1280
MluNI TGGCCA 1 cut(s) 1280
MlyI GAGTC 3 cut(s) 101, 498, 1628
MmeI TCCRAC 2 cut(s) 143, 514
Mox20I TGGCCA 1 cut(s) 1280
MroXI GAANNNNTTC 1 cut(s) 621
MscI TGGCCA 1 cut(s) 1280
MseI TTAA 7 cut(s) 90, 147, 158, 287, 912, 1035, 1422
Msp20I TGGCCA 1 cut(s) 1280
MspI CCGG 2 cut(s) 206, 668
MspR9I CCNGG 3 cut(s) 11, 810, 1373
MunI CAATTG 2 cut(s) 1008, 1434
MvaI CCWGG 3 cut(s) 11, 810, 1373
MwoI GCNNNNNNNGC 2 cut(s) 722, 1609
NdeI CATATG 1 cut(s) 194
NdeII GATC 5 cut(s) 74, 361, 409, 896, 1167
NlaIII CATG 6 cut(s) 46, 339, 491, 746, 1123, 1421
NlaIV GGNNCC 2 cut(s) 363, 981
NmuCI GTSAC 3 cut(s) 771, 845, 1631
NspI RCATGY 1 cut(s) 339
PaeR7I CTCGAG 1 cut(s) 1163
PagI TCATGA 2 cut(s) 42, 1119
PdmI GAANNNNTTC 1 cut(s) 621
PfeI GAWTC 3 cut(s) 537, 1095, 1484
PflFI GACNNNGTC 1 cut(s) 1629
PflMI CCANNNNNTGG 1 cut(s) 794
PkrI GCNGC 3 cut(s) 297, 710, 1312
PleI GAGTC 3 cut(s) 101, 498, 1627
PpsI GAGTC 3 cut(s) 101, 498, 1627
PshAI GACNNNNGTC 1 cut(s) 1462
Psp1406I AACGTT 2 cut(s) 29, 961
Psp6I CCWGG 3 cut(s) 9, 808, 1371
PspEI GGTNACC 1 cut(s) 845
PspGI CCWGG 3 cut(s) 9, 808, 1371
PspN4I GGNNCC 2 cut(s) 363, 981
PspPI GGNCC 5 cut(s) 62, 215, 320, 665, 1223
PsuI RGATCY 2 cut(s) 361, 896
PsyI GACNNNGTC 1 cut(s) 1629
RsaI GTAC 1 cut(s) 1331
RsaNI GTAC 1 cut(s) 1330
SaqAI TTAA 7 cut(s) 90, 147, 158, 287, 912, 1035, 1422
SatI GCNGC 3 cut(s) 296, 709, 1311
Sau3AI GATC 5 cut(s) 74, 361, 409, 896, 1167
Sau96I GGNCC 5 cut(s) 62, 215, 320, 665, 1223
SchI GAGTC 3 cut(s) 101, 498, 1628
ScrFI CCNGG 3 cut(s) 11, 810, 1373
SduI GDGCHC 1 cut(s) 393
SfaNI GCATC 2 cut(s) 36, 1077
SfcI CTRYAG 1 cut(s) 652
Sfr274I CTCGAG 1 cut(s) 1163
SinI GGWCC 4 cut(s) 215, 320, 665, 1223
SlaI CTCGAG 1 cut(s) 1163
SmlI CTYRAG 2 cut(s) 986, 1163
SmoI CTYRAG 2 cut(s) 986, 1163
SsiI CCGC 5 cut(s) 65, 296, 716, 1308, 1391
SspI AATATT 1 cut(s) 1192
SspMI CTAG 1 cut(s) 1049
StyD4I CCNGG 3 cut(s) 9, 808, 1371
StyI CCWWGG 1 cut(s) 177
TaaI ACNGT 5 cut(s) 129, 591, 777, 1461, 1629
TaiI ACGT 2 cut(s) 32, 964
TaqI TCGA 8 cut(s) 115, 399, 636, 805, 957, 1071, 1164, 1386
TauI GCSGC 1 cut(s) 298
TfiI GAWTC 3 cut(s) 537, 1095, 1484
Tru1I TTAA 7 cut(s) 90, 147, 158, 287, 912, 1035, 1422
Tru9I TTAA 7 cut(s) 90, 147, 158, 287, 912, 1035, 1422
TscAI CASTG 3 cut(s) 1447, 1466, 1634
TseFI GTSAC 3 cut(s) 771, 845, 1631
TseI GCWGC 2 cut(s) 708, 1310
Tsp45I GTSAC 3 cut(s) 771, 845, 1631
TspGWI ACGGA 5 cut(s) 144, 333, 374, 1317, 1568
TspRI CASTG 3 cut(s) 1447, 1466, 1634
Tth111I GACNNNGTC 1 cut(s) 1629
Van91I CCANNNNNTGG 1 cut(s) 794
VpaK11BI GGWCC 4 cut(s) 215, 320, 665, 1223
XagI CCTNNNNNAGG 1 cut(s) 1641
XapI RAATTY 3 cut(s) 86, 167, 1001
XceI RCATGY 1 cut(s) 339
XhoI CTCGAG 1 cut(s) 1163
XmnI GAANNNNTTC 1 cut(s) 621
XspI CTAG 1 cut(s) 1049
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.