Rh4AG024500

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0004882
Physical Location & Seq
Forward (+)
4762495 .. 4765005
2511 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG024500.1

Sequence Viewer

Length: 720 bp
ATGGAGGAGACAGTGCAGGGACAGAACCAGACTGTGCCGTTTCAAGGTGTCGGTGGATATAGTGGCTGGCTGGAATACAATTACGATCAATCCAAGGGGTTTTATGATGGGGAGCTTGGCTTGAATGATGGAGAAAGAAAAATGCCTGTTTTCTTTATCCCCTTCCAAGACTTGGAAACAGCAAGGAATAGACAAATTCGGGAGCTAGGAGAGGCTGTTGTGCAGATGCAAATGTCTAATGCTACTTATAGTGCACTCCATGAGAGCTCTAGTTTTGTTTTATGCAATGATGAAGTGAAATTAGTGAAGATTGATGGAAATTTGGAGAACGTGGAAGAGAATATTCGGTATCGGAGATTGGTGCAAGATTTTAAGAGATTTGGACAGACCGTTCGTCAGTACTTTCTTGATCCAAGAATCATAGATTGGCAACGAAGGGACGAGTTTTTGAAAATCTTCAACTTTGCAAATATAGGGTATGATAGGCTGACCAAGATGGTGAACAGGCACCCATTTTTAATGGTAAGTGATCATTGCCGGATGAAATATTTCTCGGAGATTTGGCATACATTATCTAGTACTACTCTCAGTCAAAGGGTGTTGTATGCCTTGGGTTCAAGAGGTCTTATGCACTCCACTTATGTTCAGCAAAAAACCCATGGATGGTTTAGACCGGGCATGCGGAAGGCAGAGACAAGGACAAGGGCAGAGAGGCAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

239

Amino Acids

28.23

Weight (kDa)

8.8

Isoelectric Point (pI)

48.76

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 507
AccB7I CCANNNNNTGG 1 cut(s) 172
AciI CCGC 1 cut(s) 682
AclWI GGATC 1 cut(s) 404
AcsI RAATTY 2 cut(s) 195, 319
AfaI GTAC 2 cut(s) 401, 580
AfiI CCNNNNNNNGG 3 cut(s) 44, 172, 663
AgsI TTSAA 5 cut(s) 44, 124, 451, 460, 618
AhdI GACNNNNNGTC 1 cut(s) 393
AjuI GAANNNNNNNTTGG 2 cut(s) 409, 441
AloI GAACNNNNNNTCC 2 cut(s) 375, 407
AluBI AGCT 3 cut(s) 115, 205, 267
AluI AGCT 3 cut(s) 115, 205, 267
Alw21I GWGCWC 2 cut(s) 256, 269
Alw26I GTCTC 2 cut(s) 2, 686
Alw44I GTGCAC 1 cut(s) 252
AlwI GGATC 1 cut(s) 404
ApaLI GTGCAC 1 cut(s) 252
ApoI RAATTY 2 cut(s) 195, 319
ArsI GACNNNNNNTTYG 2 cut(s) 375, 407
Asp700I GAANNNNTTC 2 cut(s) 455, 548
AsuC2I CCSGG 1 cut(s) 675
AsuHPI GGTGA 1 cut(s) 511
BaeGI GKGCMC 1 cut(s) 256
BanI GGYRCC 1 cut(s) 507
BanII GRGCYC 1 cut(s) 269
Bbv12I GWGCWC 2 cut(s) 256, 269
BccI CCATC 5 cut(s) 101, 122, 308, 490, 657
BceAI ACGGC 1 cut(s) 22
BclI TGATCA 1 cut(s) 529
BcnI CCSGG 1 cut(s) 675
BcoDI GTCTC 2 cut(s) 2, 686
BfaI CTAG 3 cut(s) 206, 270, 576
BmcAI AGTACT 2 cut(s) 401, 580
Bme1390I CCNGG 1 cut(s) 675
BmeRI GACNNNNNGTC 1 cut(s) 393
BmiI GGNNCC 1 cut(s) 509
BmrFI CCNGG 1 cut(s) 675
BmsI GCATC 1 cut(s) 216
BpuMI CCSGG 1 cut(s) 675
BsaJI CCNNGG 3 cut(s) 93, 609, 658
BsaXI ACNNNNNCTCC 2 cut(s) 201, 231
Bsc4I CCNNNNNNNGG 3 cut(s) 44, 172, 663
Bse3DI GCAATG 2 cut(s) 292, 532
BseDI CCNNGG 3 cut(s) 93, 609, 658
BseGI GGATG 2 cut(s) 546, 668
BseLI CCNNNNNNNGG 3 cut(s) 44, 172, 663
BseMI GCAATG 2 cut(s) 292, 532
BseMII CTCAG 1 cut(s) 601
BseRI GAGGAG 1 cut(s) 20
BseSI GKGCMC 1 cut(s) 256
BsgI GTGCAG 2 cut(s) 35, 242
BshNI GGYRCC 1 cut(s) 507
BsiHKAI GWGCWC 2 cut(s) 256, 269
BsiSI CCGG 2 cut(s) 538, 674
BslFI GGGAC 2 cut(s) 33, 452
BslI CCNNNNNNNGG 3 cut(s) 44, 172, 663
BsmAI GTCTC 2 cut(s) 2, 686
BsmFI GGGAC 2 cut(s) 33, 452
Bsp1286I GDGCHC 2 cut(s) 256, 269
Bsp143I GATC 3 cut(s) 85, 409, 529
Bsp19I CCATGG 1 cut(s) 658
BspACI CCGC 1 cut(s) 682
BspCNI CTCAG 1 cut(s) 600
BspLI GGNNCC 1 cut(s) 509
BspPI GGATC 1 cut(s) 404
BspT107I GGYRCC 1 cut(s) 507
BsrDI GCAATG 2 cut(s) 292, 532
BssECI CCNNGG 3 cut(s) 93, 609, 658
BssMI GATC 3 cut(s) 85, 409, 529
BssT1I CCWWGG 3 cut(s) 93, 609, 658
Bst4CI ACNGT 3 cut(s) 13, 34, 391
Bst6I CTCTTC 1 cut(s) 330
BstC8I GCNNGC 2 cut(s) 68, 680
BstDEI CTNAG 1 cut(s) 587
BstDSI CCRYGG 1 cut(s) 658
BstF5I GGATG 2 cut(s) 546, 668
BstKTI GATC 3 cut(s) 88, 412, 532
BstMAI GTCTC 2 cut(s) 2, 686
BstMBI GATC 3 cut(s) 85, 409, 529
BstNSI RCATGY 1 cut(s) 682
BstSCI CCNGG 1 cut(s) 673
BstSLI GKGCMC 1 cut(s) 256
BtgI CCRYGG 1 cut(s) 658
BtsCI GGATG 2 cut(s) 546, 668
BtsIMutI CAGTG 1 cut(s) 18
Cac8I GCNNGC 2 cut(s) 68, 680
Csp6I GTAC 2 cut(s) 400, 579
CviAII CATG 3 cut(s) 260, 659, 679
CviJI RGCY 8 cut(s) 66, 70, 115, 120, 205, 215, 267, 487
CviKI_1 RGCY 8 cut(s) 66, 70, 115, 120, 205, 215, 267, 487
CviQI GTAC 2 cut(s) 400, 579
DdeI CTNAG 1 cut(s) 587
DpnI GATC 3 cut(s) 87, 411, 531
DpnII GATC 3 cut(s) 85, 409, 529
DriI GACNNNNNGTC 1 cut(s) 393
Eam1104I CTCTTC 1 cut(s) 330
Eam1105I GACNNNNNGTC 1 cut(s) 393
EarI CTCTTC 1 cut(s) 330
Ecl136II GAGCTC 1 cut(s) 267
Eco130I CCWWGG 3 cut(s) 93, 609, 658
Eco24I GRGCYC 1 cut(s) 269
Eco53kI GAGCTC 1 cut(s) 267
EcoICRI GAGCTC 1 cut(s) 267
EcoT14I CCWWGG 3 cut(s) 93, 609, 658
EcoT38I GRGCYC 1 cut(s) 269
ErhI CCWWGG 3 cut(s) 93, 609, 658
FaeI CATG 3 cut(s) 263, 662, 682
FaqI GGGAC 2 cut(s) 33, 452
FatI CATG 3 cut(s) 259, 658, 678
FbaI TGATCA 1 cut(s) 529
FokI GGATG 2 cut(s) 553, 675
FriOI GRGCYC 1 cut(s) 269
FspBI CTAG 3 cut(s) 206, 270, 576
HapII CCGG 2 cut(s) 538, 674
Hin1II CATG 3 cut(s) 263, 662, 682
HinfI GANTC 1 cut(s) 417
HpaII CCGG 2 cut(s) 538, 674
HphI GGTGA 1 cut(s) 511
Hpy166II GTNNAC 2 cut(s) 254, 502
Hpy188I TCNGA 2 cut(s) 354, 556
Hpy188III TCNNGA 3 cut(s) 200, 407, 618
Hpy8I GTNNAC 2 cut(s) 254, 502
HpyAV CCTTC 3 cut(s) 172, 429, 679
HpyCH4III ACNGT 3 cut(s) 13, 34, 391
HpyCH4IV ACGT 1 cut(s) 330
HpyCH4V TGCA 8 cut(s) 16, 223, 229, 254, 285, 364, 467, 631
HpyF3I CTNAG 1 cut(s) 587
HpySE526I ACGT 1 cut(s) 330
Hsp92II CATG 3 cut(s) 263, 662, 682
Ksp22I TGATCA 1 cut(s) 529
Kzo9I GATC 3 cut(s) 85, 409, 529
LmnI GCTCC 2 cut(s) 112, 202
LpnPI CCDG 8 cut(s) 2, 41, 52, 56, 159, 490, 551, 687
LweI GCATC 1 cut(s) 216
MaeI CTAG 3 cut(s) 206, 270, 576
MaeII ACGT 1 cut(s) 330
MalI GATC 3 cut(s) 87, 411, 531
MboI GATC 3 cut(s) 85, 409, 529
MboII GAAGA 3 cut(s) 319, 347, 448
MhlI GDGCHC 2 cut(s) 256, 269
MluCI AATT 4 cut(s) 79, 195, 299, 319
MnlI CCTC 3 cut(s) 205, 614, 705
MroXI GAANNNNTTC 2 cut(s) 455, 548
MseI TTAA 2 cut(s) 372, 518
MspI CCGG 2 cut(s) 538, 674
MspR9I CCNGG 1 cut(s) 675
NciI CCSGG 1 cut(s) 675
NcoI CCATGG 1 cut(s) 658
NdeII GATC 3 cut(s) 85, 409, 529
NlaIII CATG 3 cut(s) 263, 662, 682
NlaIV GGNNCC 1 cut(s) 509
NspI RCATGY 1 cut(s) 682
PaeI GCATGC 1 cut(s) 682
PdmI GAANNNNTTC 2 cut(s) 455, 548
PfeI GAWTC 1 cut(s) 417
PflMI CCANNNNNTGG 1 cut(s) 172
Psp124BI GAGCTC 1 cut(s) 269
PspN4I GGNNCC 1 cut(s) 509
RsaI GTAC 2 cut(s) 401, 580
RsaNI GTAC 2 cut(s) 400, 579
SacI GAGCTC 1 cut(s) 269
SaqAI TTAA 2 cut(s) 372, 518
Sau3AI GATC 3 cut(s) 85, 409, 529
ScaI AGTACT 2 cut(s) 401, 580
ScrFI CCNGG 1 cut(s) 675
SduI GDGCHC 2 cut(s) 256, 269
SetI ASST 6 cut(s) 49, 117, 207, 269, 333, 625
SfaNI GCATC 1 cut(s) 216
SphI GCATGC 1 cut(s) 682
Sse9I AATT 4 cut(s) 79, 195, 299, 319
SsiI CCGC 1 cut(s) 682
SspI AATATT 2 cut(s) 343, 548
SspMI CTAG 3 cut(s) 206, 270, 576
SstI GAGCTC 1 cut(s) 269
StyD4I CCNGG 1 cut(s) 673
StyI CCWWGG 3 cut(s) 93, 609, 658
TaaI ACNGT 3 cut(s) 13, 34, 391
TaiI ACGT 1 cut(s) 333
TasI AATT 4 cut(s) 79, 195, 299, 319
TatI WGTACW 2 cut(s) 399, 578
TfiI GAWTC 1 cut(s) 417
Tru1I TTAA 2 cut(s) 372, 518
Tru9I TTAA 2 cut(s) 372, 518
TscAI CASTG 1 cut(s) 18
TspDTI ATGAA 2 cut(s) 306, 557
TspRI CASTG 1 cut(s) 18
Van91I CCANNNNNTGG 1 cut(s) 172
VneI GTGCAC 1 cut(s) 252
XapI RAATTY 2 cut(s) 195, 319
XceI RCATGY 1 cut(s) 682
XmnI GAANNNNTTC 2 cut(s) 455, 548
XspI CTAG 3 cut(s) 206, 270, 576
ZrmI AGTACT 2 cut(s) 401, 580
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.