Rw4G001610

40S ribosomal protein S12-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0004882
Physical Location & Seq
Reverse (-)
2999179 .. 3002836
3658 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G001610.1

Sequence Viewer

Length: 1638 bp
ATGATAAACCAGGCAGAAGAAGAAGCAATGTGTCTTTATATCATGATCGAATATGGGGACGGCCCGCACTTTGGATCGTTGTATGAATTTAATATATGTAAGCAAGACTACTTTCGACCTTTAGCACAGTTCAGGGATGAGGATGTTAGCCTTCCGTTAAGTTGGAAATTTTGCTCCTTGGATACCAACAACATATGTCTATTGCCAGATAGTGGTCCTTTATCTCCTGATGATGTGGTGAACCCCTTGTCTGGATATATGTTCAATGTTGATACCAATAAATGTCATTTAATCAAGCCGCCCGTTGCTCCCAAATGGGAAGGACCTGTTGTATCTGCATGTGGGAAACTTTACTCTATTACGGATCCATGCTGCTCATTTGATGTGCCAGAGCCCTCTTTCGAGCGATATGATCCTTATAGGTGTTGTTGGGAAACTCGGAAATCTTTTCCTTATGGTAAAAATTGTTCCGAACAAAGGATTGGGGGTCATGCCGTTTGTGATGGACTCATCCTGTATAATATAATCATTCCAGGGGATAATCCATTCTTTTTCTCTAGAAAATATCAAGATGAAATGTGGGCTTATGATGTAACACTGGATGAATGGCTTCCAGTTAGGGTCGAGAACACAGATTGCTATAGTTTCCGAGGACCGGCTGTATGTGTGGACAAGACTATCTATGCGTTATCAAGCAGCTTGGCGGTTATGGCACTCTCTATCACAAGGCATGAAAATCTTGTTACCTGCTCTCTAAAGTCACAGTTTGTTTGTCCAGAGTTTGGGAACTTTCGACCAGGTGTACAGAGAATGGAGCGTTTGATTTATTTGGGTGACCTGAACTTTTGTCTTGTTCAGACTAGATTTCACTATGACAGTAGATCCAGTCAATCTCTTAAAATTACCACATTTCACCTTGTGGGAGAAACCAATATCAAAATCGAACGTTCATTCATTTATGAGGTGCCTCTTGAGGGTCATAAGAGATTCACAATTGGACATTGCTTTATCCATACTTTTAAACGCATTGAACTAGAAGAGTTGGCTAGTCGCATCGACTTCTTAAACCAAGAATACAGATTCGGCCTTCATACTGAATTCTTCATCAAGCCAGAATATGATTATCTCGAGATCGACCAGCCAAATTATGCAAATATTGAAGAAGAAAATATATCCTTACAAGGTGGACCTCATTTCAAAAACTTCGTGGACTTGTTGAAAGATTTGTTGGCAATGGCATTGGCCAATGGATTGCTCAGAAGAGGAATTCCCACAGCTTCCAATCTCATTCCGGACCAGAAGGTATGGCTGTGTGTTTTTGCTGAGGACAGCAACCAGGATGATGTTGTCAAGGCGGTAAAGTCATTGTGTGAGGGGTATCATGTTAAGTATCTGACAATTGCCAGTGCTAAAACTCTTGGACAGTGGTCTCTGTCTGGTAATAAGGATTCTGAAGGAAAGGCAAAGAGGGTGGTTCGTTGCTCTTGTGTTGTTATTGAGGAAGGCATATATGAGACGGAACTTCATAAAGCAGTTTTGGAAGTCTGGAAGTCTCTCCCATCAAAGCTGGAAGTGCTGGAAGAGTCTGGACAGTGTCGCCCTGTTGTCGGGTCAAGGGCTTCTTTAGATAGTCCATGA

Protein Analysis

545

Amino Acids

62.17

Weight (kDa)

5.23

Isoelectric Point (pI)

49.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L7Ae PF01248 407 - 498 2.5e-09 Ribosomal protein L7Ae/L30e/S12e/Gadd45 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 755
AccB1I GGYRCC 1 cut(s) 964
AccB7I CCANNNNNTGG 2 cut(s) 212, 782
AccIII TCCGGA 1 cut(s) 1291
AciI CCGC 4 cut(s) 65, 299, 704, 1355
AclI AACGTT 1 cut(s) 946
AclWI GGATC 5 cut(s) 82, 359, 372, 407, 876
AcoI YGGCCR 1 cut(s) 1242
AcsI RAATTY 4 cut(s) 86, 167, 1097, 1266
AcuI CTGAAG 1 cut(s) 1473
AdeI CACNNNGTG 1 cut(s) 919
AfaI GTAC 1 cut(s) 804
AfiI CCNNNNNNNGG 8 cut(s) 71, 212, 251, 477, 655, 782, 974, 1607
AgsI TTSAA 5 cut(s) 265, 1031, 1160, 1198, 1219
AjnI CCWGG 4 cut(s) 9, 532, 796, 1335
AjuI GAANNNNNNNTTGG 4 cut(s) 465, 497, 1211, 1243
AluBI AGCT 3 cut(s) 699, 1277, 1567
AluI AGCT 3 cut(s) 699, 1277, 1567
Alw26I GTCTC 3 cut(s) 1434, 1508, 1557
AlwI GGATC 5 cut(s) 82, 359, 372, 407, 876
Ama87I CYCGRG 1 cut(s) 1127
Aor13HI TCCGGA 1 cut(s) 1291
AoxI GGCC 3 cut(s) 61, 1084, 1242
ApeKI GCWGC 2 cut(s) 372, 696
ApoI RAATTY 4 cut(s) 86, 167, 1097, 1266
Asp700I GAANNNNTTC 1 cut(s) 609
AspS9I GGNCC 6 cut(s) 62, 215, 323, 653, 1187, 1294
AsuHPI GGTGA 3 cut(s) 250, 845, 905
AvaI CYCGRG 1 cut(s) 1127
AvaII GGWCC 5 cut(s) 215, 323, 653, 1187, 1294
BalI TGGCCA 1 cut(s) 1244
BamHI GGATCC 1 cut(s) 364
BanI GGYRCC 1 cut(s) 964
BanII GRGCYC 1 cut(s) 396
BbvCI CCTCAGC 1 cut(s) 1323
BbvI GCAGC 2 cut(s) 359, 708
BccI CCATC 2 cut(s) 497, 1567
BceAI ACGGC 2 cut(s) 76, 479
BciT130I CCWGG 4 cut(s) 11, 534, 798, 1337
BciVI GTATCC 1 cut(s) 175
BcoDI GTCTC 3 cut(s) 1434, 1508, 1557
BfaI CTAG 4 cut(s) 558, 861, 1034, 1047
BfmI CTRYAG 1 cut(s) 640
BfuAI ACCTGC 1 cut(s) 755
BfuI GTATCC 1 cut(s) 175
BisI GCNGC 3 cut(s) 299, 373, 697
BlsI GCNGC 3 cut(s) 300, 374, 698
Bme1390I CCNGG 4 cut(s) 11, 534, 798, 1337
Bme18I GGWCC 5 cut(s) 215, 323, 653, 1187, 1294
BmeT110I CYCGRG 1 cut(s) 1127
BmgT120I GGNCC 6 cut(s) 62, 215, 323, 653, 1187, 1294
BmiI GGNNCC 2 cut(s) 366, 966
BmrFI CCNGG 4 cut(s) 11, 534, 798, 1337
BmsI GCATC 1 cut(s) 1062
BoxI GACNNNNGTC 1 cut(s) 1426
Bpu10I CCTNAGC 1 cut(s) 1323
BpuEI CTTGAG 1 cut(s) 992
BsaI GGTCTC 1 cut(s) 1434
BsaJI CCNNGG 3 cut(s) 177, 533, 649
BsaWI WCCGGW 1 cut(s) 1291
Bsc4I CCNNNNNNNGG 8 cut(s) 71, 212, 251, 477, 655, 782, 974, 1607
Bse118I RCCGGY 1 cut(s) 655
Bse1I ACTGG 4 cut(s) 603, 614, 885, 1404
Bse3DI GCAATG 3 cut(s) 33, 1000, 1239
BseAI TCCGGA 1 cut(s) 1291
BseBI CCWGG 4 cut(s) 11, 534, 798, 1337
BseDI CCNNGG 3 cut(s) 177, 533, 649
BseGI GGATG 5 cut(s) 142, 148, 510, 607, 1345
BseLI CCNNNNNNNGG 8 cut(s) 71, 212, 251, 477, 655, 782, 974, 1607
BseMI GCAATG 3 cut(s) 33, 1000, 1239
BseMII CTCAG 2 cut(s) 1270, 1314
BseNI ACTGG 4 cut(s) 603, 614, 885, 1404
BseXI GCAGC 2 cut(s) 359, 708
BshFI GGCC 3 cut(s) 63, 1086, 1244
BshNI GGYRCC 1 cut(s) 964
BsiHKCI CYCGRG 1 cut(s) 1127
BsiSI CCGG 2 cut(s) 656, 1292
BslFI GGGAC 1 cut(s) 71
BslI CCNNNNNNNGG 8 cut(s) 71, 212, 251, 477, 655, 782, 974, 1607
BsmAI GTCTC 3 cut(s) 1434, 1508, 1557
BsmBI CGTCTC 1 cut(s) 1508
BsmFI GGGAC 1 cut(s) 71
BsnI GGCC 3 cut(s) 63, 1086, 1244
Bso31I GGTCTC 1 cut(s) 1434
BsoBI CYCGRG 1 cut(s) 1127
Bsp1286I GDGCHC 1 cut(s) 396
Bsp13I TCCGGA 1 cut(s) 1291
Bsp1407I TGTACA 1 cut(s) 802
Bsp143I GATC 6 cut(s) 45, 74, 364, 412, 881, 1131
BspACI CCGC 4 cut(s) 65, 299, 704, 1355
BspANI GGCC 3 cut(s) 63, 1086, 1244
BspCNI CTCAG 2 cut(s) 1269, 1315
BspEI TCCGGA 1 cut(s) 1291
BspHI TCATGA 1 cut(s) 42
BspLI GGNNCC 2 cut(s) 366, 966
BspMI ACCTGC 1 cut(s) 755
BspPI GGATC 5 cut(s) 82, 359, 372, 407, 876
BspT107I GGYRCC 1 cut(s) 964
BspTNI GGTCTC 1 cut(s) 1434
BsrDI GCAATG 3 cut(s) 33, 1000, 1239
BsrFI RCCGGY 1 cut(s) 655
BsrGI TGTACA 1 cut(s) 802
BsrI ACTGG 4 cut(s) 603, 614, 885, 1404
BssAI RCCGGY 1 cut(s) 655
BssECI CCNNGG 3 cut(s) 177, 533, 649
BssMI GATC 6 cut(s) 45, 74, 364, 412, 881, 1131
BssT1I CCWWGG 1 cut(s) 177
Bst2UI CCWGG 4 cut(s) 11, 534, 798, 1337
Bst4CI ACNGT 5 cut(s) 129, 765, 878, 1425, 1593
Bst6I CTCTTC 3 cut(s) 1032, 1255, 1575
BstAUI TGTACA 1 cut(s) 802
BstC8I GCNNGC 1 cut(s) 65
BstDEI CTNAG 2 cut(s) 1256, 1323
BstEII GGTNACC 1 cut(s) 833
BstF5I GGATG 5 cut(s) 142, 148, 510, 607, 1345
BstKTI GATC 6 cut(s) 48, 77, 367, 415, 884, 1134
BstMAI GTCTC 3 cut(s) 1434, 1508, 1557
BstMBI GATC 6 cut(s) 45, 74, 364, 412, 881, 1131
BstMWI GCNNNNNNNGC 2 cut(s) 710, 1573
BstNI CCWGG 4 cut(s) 11, 534, 798, 1337
BstNSI RCATGY 1 cut(s) 342
BstPAI GACNNNNGTC 1 cut(s) 1426
BstPI GGTNACC 1 cut(s) 833
BstSCI CCNGG 4 cut(s) 9, 532, 796, 1335
BstSFI CTRYAG 1 cut(s) 640
BstV1I GCAGC 2 cut(s) 359, 708
BstX2I RGATCY 2 cut(s) 364, 881
BstYI RGATCY 2 cut(s) 364, 881
BsuI GTATCC 1 cut(s) 175
BsuRI GGCC 3 cut(s) 63, 1086, 1244
BtsCI GGATG 5 cut(s) 142, 148, 510, 607, 1345
BtsIMutI CAGTG 4 cut(s) 596, 1411, 1430, 1598
BveI ACCTGC 1 cut(s) 755
Cac8I GCNNGC 1 cut(s) 65
CciI TCATGA 1 cut(s) 42
Cfr10I RCCGGY 1 cut(s) 655
Cfr13I GGNCC 6 cut(s) 62, 215, 323, 653, 1187, 1294
CsiI ACCWGGT 1 cut(s) 796
Csp6I GTAC 1 cut(s) 803
CspCI CAANNNNNGTGG 2 cut(s) 1452, 1487
CviAII CATG 7 cut(s) 43, 339, 369, 491, 731, 1382, 1635
CviQI GTAC 1 cut(s) 803
DdeI CTNAG 2 cut(s) 1256, 1323
DpnI GATC 6 cut(s) 47, 76, 366, 414, 883, 1133
DpnII GATC 6 cut(s) 45, 74, 364, 412, 881, 1131
DraI TTTAAA 1 cut(s) 1021
DraIII CACNNNGTG 1 cut(s) 919
EaeI YGGCCR 1 cut(s) 1242
Eam1104I CTCTTC 3 cut(s) 1032, 1255, 1575
EarI CTCTTC 3 cut(s) 1032, 1255, 1575
Eco130I CCWWGG 1 cut(s) 177
Eco24I GRGCYC 1 cut(s) 396
Eco31I GGTCTC 1 cut(s) 1434
Eco47I GGWCC 5 cut(s) 215, 323, 653, 1187, 1294
Eco57I CTGAAG 1 cut(s) 1473
Eco88I CYCGRG 1 cut(s) 1127
Eco91I GGTNACC 1 cut(s) 833
EcoO109I RGGNCCY 1 cut(s) 323
EcoO65I GGTNACC 1 cut(s) 833
EcoRI GAATTC 2 cut(s) 1097, 1266
EcoRII CCWGG 4 cut(s) 9, 532, 796, 1335
EcoT14I CCWWGG 1 cut(s) 177
EcoT38I GRGCYC 1 cut(s) 396
ErhI CCWWGG 1 cut(s) 177
Esp3I CGTCTC 1 cut(s) 1508
FaeI CATG 7 cut(s) 46, 342, 372, 494, 734, 1385, 1638
FalI AAGNNNNNCTT 2 cut(s) 1606, 1638
FaqI GGGAC 1 cut(s) 71
FatI CATG 7 cut(s) 42, 338, 368, 490, 730, 1381, 1634
FauI CCCGC 1 cut(s) 72
FauNDI CATATG 1 cut(s) 194
Fnu4HI GCNGC 3 cut(s) 299, 373, 697
FokI GGATG 5 cut(s) 149, 155, 497, 614, 1352
FriOI GRGCYC 1 cut(s) 396
Fsp4HI GCNGC 3 cut(s) 299, 373, 697
FspBI CTAG 4 cut(s) 558, 861, 1034, 1047
GluI GCNGC 3 cut(s) 299, 373, 697
HaeIII GGCC 3 cut(s) 63, 1086, 1244
HapII CCGG 2 cut(s) 656, 1292
Hin1II CATG 7 cut(s) 46, 342, 372, 494, 734, 1385, 1638
HinfI GANTC 5 cut(s) 507, 987, 1080, 1448, 1583
HpaII CCGG 2 cut(s) 656, 1292
HphI GGTGA 3 cut(s) 250, 845, 905
Hpy166II GTNNAC 5 cut(s) 241, 670, 803, 1187, 1210
Hpy188I TCNGA 7 cut(s) 441, 472, 650, 858, 1259, 1395, 1453
Hpy8I GTNNAC 5 cut(s) 241, 670, 803, 1187, 1210
HpyAV CCTTC 6 cut(s) 161, 314, 1097, 1294, 1448, 1496
HpyCH4III ACNGT 5 cut(s) 129, 765, 878, 1425, 1593
HpyCH4IV ACGT 1 cut(s) 946
HpyCH4V TGCA 2 cut(s) 338, 1151
HpyF10VI GCNNNNNNNGC 2 cut(s) 710, 1573
HpyF3I CTNAG 2 cut(s) 1256, 1323
HpySE526I ACGT 1 cut(s) 946
Hsp92II CATG 7 cut(s) 46, 342, 372, 494, 734, 1385, 1638
Kpn2I TCCGGA 1 cut(s) 1291
Kzo9I GATC 6 cut(s) 45, 74, 364, 412, 881, 1131
LmnI GCTCC 3 cut(s) 179, 313, 814
Lsp1109I GCAGC 2 cut(s) 359, 708
LweI GCATC 1 cut(s) 1062
MabI ACCWGGT 1 cut(s) 796
MaeI CTAG 4 cut(s) 558, 861, 1034, 1047
MaeII ACGT 1 cut(s) 946
MaeIII GTNAC 4 cut(s) 592, 742, 759, 833
MalI GATC 6 cut(s) 47, 76, 366, 414, 883, 1133
MboI GATC 6 cut(s) 45, 74, 364, 412, 881, 1131
MboII GAAGA 8 cut(s) 29, 32, 1049, 1093, 1172, 1175, 1272, 1592
MfeI CAATTG 2 cut(s) 993, 1398
MflI RGATCY 2 cut(s) 364, 881
MhlI GDGCHC 1 cut(s) 396
MlsI TGGCCA 1 cut(s) 1244
MluCI AATT 9 cut(s) 86, 167, 463, 900, 993, 1097, 1144, 1266, 1398
MluNI TGGCCA 1 cut(s) 1244
MlyI GAGTC 2 cut(s) 501, 1592
MmeI TCCRAC 1 cut(s) 143
Mox20I TGGCCA 1 cut(s) 1244
MroI TCCGGA 1 cut(s) 1291
MroXI GAANNNNTTC 1 cut(s) 609
MscI TGGCCA 1 cut(s) 1244
MseI TTAA 7 cut(s) 90, 158, 290, 897, 1020, 1064, 1386
Msp20I TGGCCA 1 cut(s) 1244
MspI CCGG 2 cut(s) 656, 1292
MspR9I CCNGG 4 cut(s) 11, 534, 798, 1337
MunI CAATTG 2 cut(s) 993, 1398
MvaI CCWGG 4 cut(s) 11, 534, 798, 1337
MwoI GCNNNNNNNGC 2 cut(s) 710, 1573
NdeI CATATG 1 cut(s) 194
NdeII GATC 6 cut(s) 45, 74, 364, 412, 881, 1131
NlaIII CATG 7 cut(s) 46, 342, 372, 494, 734, 1385, 1638
NlaIV GGNNCC 2 cut(s) 366, 966
NmuCI GTSAC 2 cut(s) 759, 833
NspI RCATGY 1 cut(s) 342
PaeR7I CTCGAG 1 cut(s) 1127
PagI TCATGA 1 cut(s) 42
PdmI GAANNNNTTC 1 cut(s) 609
PfeI GAWTC 3 cut(s) 987, 1080, 1448
PflFI GACNNNGTC 1 cut(s) 1593
PflMI CCANNNNNTGG 2 cut(s) 212, 782
PkrI GCNGC 3 cut(s) 300, 374, 698
PleI GAGTC 2 cut(s) 501, 1591
PpsI GAGTC 2 cut(s) 501, 1591
PpuMI RGGWCCY 1 cut(s) 323
PshAI GACNNNNGTC 1 cut(s) 1426
Psp1406I AACGTT 1 cut(s) 946
Psp5II RGGWCCY 1 cut(s) 323
Psp6I CCWGG 4 cut(s) 9, 532, 796, 1335
PspEI GGTNACC 1 cut(s) 833
PspGI CCWGG 4 cut(s) 9, 532, 796, 1335
PspN4I GGNNCC 2 cut(s) 366, 966
PspPI GGNCC 6 cut(s) 62, 215, 323, 653, 1187, 1294
PspPPI RGGWCCY 1 cut(s) 323
PsrI GAACNNNNNNTAC 2 cut(s) 451, 483
PsuI RGATCY 2 cut(s) 364, 881
PsyI GACNNNGTC 1 cut(s) 1593
RsaI GTAC 1 cut(s) 804
RsaNI GTAC 1 cut(s) 803
SaqAI TTAA 7 cut(s) 90, 158, 290, 897, 1020, 1064, 1386
SatI GCNGC 3 cut(s) 299, 373, 697
Sau3AI GATC 6 cut(s) 45, 74, 364, 412, 881, 1131
Sau96I GGNCC 6 cut(s) 62, 215, 323, 653, 1187, 1294
SchI GAGTC 2 cut(s) 501, 1592
ScrFI CCNGG 4 cut(s) 11, 534, 798, 1337
SduI GDGCHC 1 cut(s) 396
SexAI ACCWGGT 1 cut(s) 796
SfaNI GCATC 1 cut(s) 1062
SfcI CTRYAG 1 cut(s) 640
Sfr274I CTCGAG 1 cut(s) 1127
SinI GGWCC 5 cut(s) 215, 323, 653, 1187, 1294
SlaI CTCGAG 1 cut(s) 1127
SmlI CTYRAG 2 cut(s) 971, 1127
SmoI CTYRAG 2 cut(s) 971, 1127
Sse9I AATT 9 cut(s) 86, 167, 463, 900, 993, 1097, 1144, 1266, 1398
SsiI CCGC 4 cut(s) 65, 299, 704, 1355
SspI AATATT 1 cut(s) 1156
SspMI CTAG 4 cut(s) 558, 861, 1034, 1047
StyD4I CCNGG 4 cut(s) 9, 532, 796, 1335
StyI CCWWGG 1 cut(s) 177
TaaI ACNGT 5 cut(s) 129, 765, 878, 1425, 1593
TaiI ACGT 1 cut(s) 949
TaqI TCGA 9 cut(s) 48, 115, 402, 624, 793, 942, 1056, 1128, 1134
TasI AATT 9 cut(s) 86, 167, 463, 900, 993, 1097, 1144, 1266, 1398
TatI WGTACW 1 cut(s) 802
TauI GCSGC 1 cut(s) 301
TfiI GAWTC 3 cut(s) 987, 1080, 1448
Tru1I TTAA 7 cut(s) 90, 158, 290, 897, 1020, 1064, 1386
Tru9I TTAA 7 cut(s) 90, 158, 290, 897, 1020, 1064, 1386
TscAI CASTG 4 cut(s) 603, 1411, 1430, 1598
TseFI GTSAC 2 cut(s) 759, 833
TseI GCWGC 2 cut(s) 372, 696
Tsp45I GTSAC 2 cut(s) 759, 833
TspDTI ATGAA 9 cut(s) 99, 588, 618, 747, 939, 943, 1079, 1093, 1514
TspGWI ACGGA 3 cut(s) 144, 377, 1532
TspRI CASTG 4 cut(s) 603, 1411, 1430, 1598
Tth111I GACNNNGTC 1 cut(s) 1593
Van91I CCANNNNNTGG 2 cut(s) 212, 782
VpaK11BI GGWCC 5 cut(s) 215, 323, 653, 1187, 1294
XapI RAATTY 4 cut(s) 86, 167, 1097, 1266
XbaI TCTAGA 1 cut(s) 557
XceI RCATGY 1 cut(s) 342
XhoI CTCGAG 1 cut(s) 1127
XmnI GAANNNNTTC 1 cut(s) 609
XspI CTAG 4 cut(s) 558, 861, 1034, 1047
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.