Rh4AG022900

40S ribosomal protein S12-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0004882
Physical Location & Seq
Reverse (-)
4469463 .. 4473094
3632 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG022900.1

Sequence Viewer

Length: 1632 bp
ATGTGTCTTTATATCATGATCGAATATGGGGACGGCCCGCACTTTGGATCGTTGTATGAATTTAATATATGTAAGCAAGACTACTTTCGACCTTTAGCACAGTTCAGGGATGAGGATGTTAGCCTTCCGTTAAGTTGGAAATTTTGCTCCTTGGATACCAACAACATATGTCTATTGCCAGATAGTGGTCCTTTATCTCCTGATGATGTGGTGAACCCCTTGTCTGGATATATGTTCAATGTTGATACCAATAAATGTCATTTAATCAAGCCGCCCGTTGCTCCCAAATGGGAAGGACCTGTTGTATCTGCATGTGGGAAACTTTACTCTATTACGGATCCATGCTGCTCATTTGATGTGCCAGAGCCCTCTTTCGAGCGATATGATCCTTATAGGTGTTGTTGGGAAACTCGGAAATCTTTTCCTTATGGTAAAAATTGTTCCGAACAAAGGATTGGGGGTCATGCCGTTTGTGATGGACTCATCCTGTATAATATAATCATTCCAGGGGATAATCCATTCTTTTTCTCTAGAAAATATCAAGATGAAATGTGGGCTTATGATGTAACACTGGATGAATGGCTTCCAGTTAGGGTCGAGAACACAGATTGCTATAGTTTCCGAGGACCGGCTGTATGTGTGGACAAGACTATCTATGCGTTATCAAGCAGCTTGGCGGTTATGGCACTCTCTATCACAAGGCATGAAAATCTTGTTACCTGCTCTCTAAAGTCACAGTTTGTTTGTCCAGAGTTTGGGAACTTTCGACCAGGTGTACAGAGAATGGAGCGTTTGATTTATTTGGGTGACCTGAACTTTTGTCTTGTTCAGACTAGATTTCACTATGACAGTAGATCCAGTCAATCTCTTAAAATTACCACATTTCACCTTGTGGGAGAAACCAATATCAAAATCGAATGTTCATTCATTTATGAGGTGCCTCTTGAGGGTCATAAGAGATTCACAATTGGACATTGCTTTATCCATACTTTTAAACGCATTGAACTAGAAGAGTTGGCTAGTCGCATCGACTTCTTAAACCAAGAATACAGATTCGGCCTTCATACTGAATTCTTCATCAAGCCTGTTGACTTCATATTCAAGCCAGAATATGATTATCTCGAGATCGACCAGCCAAATTATGCAAATATTGAAGAAGAAAATATATCCTTACAAGGTGGACCTCATTTCAAAAACTTCGTGGACTTGTTGAAAGATTTGTTGGCAATGGCATTGGCCAATGGATTGCTCAGAAGAGGAATTCCCACAGCTTCCAATCTCATTCCGGACCAGAAGGTATGGCTGTGTGTTTTTGCTGAGGACAGCAACCAGGATGATGTTGTCAAGGCGGTAAAGTCATTGTGTGAGGGGTATCATGTTAAGTATCTGACAATTGCCAGTGCTAAAACTCTTGGACAGTGGTCTCTGTCTGGTAATAAGGATTCTGAAGGAAAGGCAAAGAGGGTGGTTCGTTGCTCTTGTGTTGTTATTGAGGAAGGCATATATGAGACGGAACTTCATAAAGCAGTTTTGGAAGTCTGGAAGTCTCTCCCATCAAAGCTGGAAGTGCTGGAAGAGTCTGGACAGTGTCGCCCTGTTGTCGGGTCAAGGGCTTCTTTAGATAGTCCATGA

Protein Analysis

543

Amino Acids

61.94

Weight (kDa)

5.32

Isoelectric Point (pI)

47.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L7Ae PF01248 405 - 496 2.5e-09 Ribosomal protein L7Ae/L30e/S12e/Gadd45 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 728
AccB1I GGYRCC 1 cut(s) 937
AccB7I CCANNNNNTGG 2 cut(s) 185, 755
AccIII TCCGGA 1 cut(s) 1285
AciI CCGC 4 cut(s) 38, 272, 677, 1349
AclWI GGATC 5 cut(s) 55, 332, 345, 380, 849
AcoI YGGCCR 1 cut(s) 1236
AcsI RAATTY 4 cut(s) 59, 140, 1070, 1260
AcuI CTGAAG 1 cut(s) 1467
AdeI CACNNNGTG 1 cut(s) 892
AfaI GTAC 1 cut(s) 777
AfiI CCNNNNNNNGG 8 cut(s) 44, 185, 224, 450, 628, 755, 947, 1601
AgsI TTSAA 6 cut(s) 238, 1004, 1102, 1154, 1192, 1213
AjnI CCWGG 3 cut(s) 505, 769, 1329
AjuI GAANNNNNNNTTGG 4 cut(s) 438, 470, 1205, 1237
AluBI AGCT 3 cut(s) 672, 1271, 1561
AluI AGCT 3 cut(s) 672, 1271, 1561
Alw26I GTCTC 3 cut(s) 1428, 1502, 1551
AlwI GGATC 5 cut(s) 55, 332, 345, 380, 849
Ama87I CYCGRG 1 cut(s) 1121
Aor13HI TCCGGA 1 cut(s) 1285
AoxI GGCC 3 cut(s) 34, 1057, 1236
ApeKI GCWGC 2 cut(s) 345, 669
ApoI RAATTY 4 cut(s) 59, 140, 1070, 1260
Asp700I GAANNNNTTC 1 cut(s) 582
AspS9I GGNCC 6 cut(s) 35, 188, 296, 626, 1181, 1288
AsuHPI GGTGA 3 cut(s) 223, 818, 878
AvaI CYCGRG 1 cut(s) 1121
AvaII GGWCC 5 cut(s) 188, 296, 626, 1181, 1288
BalI TGGCCA 1 cut(s) 1238
BamHI GGATCC 1 cut(s) 337
BanI GGYRCC 1 cut(s) 937
BanII GRGCYC 1 cut(s) 369
BbvCI CCTCAGC 1 cut(s) 1317
BbvI GCAGC 2 cut(s) 332, 681
BccI CCATC 2 cut(s) 470, 1561
BceAI ACGGC 2 cut(s) 49, 452
BciT130I CCWGG 3 cut(s) 507, 771, 1331
BciVI GTATCC 1 cut(s) 148
BcoDI GTCTC 3 cut(s) 1428, 1502, 1551
BfaI CTAG 4 cut(s) 531, 834, 1007, 1020
BfmI CTRYAG 1 cut(s) 613
BfuAI ACCTGC 1 cut(s) 728
BfuI GTATCC 1 cut(s) 148
BisI GCNGC 3 cut(s) 272, 346, 670
BlsI GCNGC 3 cut(s) 273, 347, 671
Bme1390I CCNGG 3 cut(s) 507, 771, 1331
Bme18I GGWCC 5 cut(s) 188, 296, 626, 1181, 1288
BmeT110I CYCGRG 1 cut(s) 1121
BmgT120I GGNCC 6 cut(s) 35, 188, 296, 626, 1181, 1288
BmiI GGNNCC 2 cut(s) 339, 939
BmrFI CCNGG 3 cut(s) 507, 771, 1331
BmsI GCATC 1 cut(s) 1035
BoxI GACNNNNGTC 1 cut(s) 1420
Bpu10I CCTNAGC 1 cut(s) 1317
BpuEI CTTGAG 1 cut(s) 965
BsaI GGTCTC 1 cut(s) 1428
BsaJI CCNNGG 3 cut(s) 150, 506, 622
BsaWI WCCGGW 1 cut(s) 1285
Bsc4I CCNNNNNNNGG 8 cut(s) 44, 185, 224, 450, 628, 755, 947, 1601
Bse118I RCCGGY 1 cut(s) 628
Bse1I ACTGG 4 cut(s) 576, 587, 858, 1398
Bse3DI GCAATG 2 cut(s) 973, 1233
BseAI TCCGGA 1 cut(s) 1285
BseBI CCWGG 3 cut(s) 507, 771, 1331
BseDI CCNNGG 3 cut(s) 150, 506, 622
BseGI GGATG 5 cut(s) 115, 121, 483, 580, 1339
BseLI CCNNNNNNNGG 8 cut(s) 44, 185, 224, 450, 628, 755, 947, 1601
BseMI GCAATG 2 cut(s) 973, 1233
BseMII CTCAG 2 cut(s) 1264, 1308
BseNI ACTGG 4 cut(s) 576, 587, 858, 1398
BseXI GCAGC 2 cut(s) 332, 681
BshFI GGCC 3 cut(s) 36, 1059, 1238
BshNI GGYRCC 1 cut(s) 937
BsiHKCI CYCGRG 1 cut(s) 1121
BsiSI CCGG 2 cut(s) 629, 1286
BslFI GGGAC 1 cut(s) 44
BslI CCNNNNNNNGG 8 cut(s) 44, 185, 224, 450, 628, 755, 947, 1601
BsmAI GTCTC 3 cut(s) 1428, 1502, 1551
BsmBI CGTCTC 1 cut(s) 1502
BsmFI GGGAC 1 cut(s) 44
BsnI GGCC 3 cut(s) 36, 1059, 1238
Bso31I GGTCTC 1 cut(s) 1428
BsoBI CYCGRG 1 cut(s) 1121
Bsp1286I GDGCHC 1 cut(s) 369
Bsp13I TCCGGA 1 cut(s) 1285
Bsp1407I TGTACA 1 cut(s) 775
Bsp143I GATC 6 cut(s) 18, 47, 337, 385, 854, 1125
BspACI CCGC 4 cut(s) 38, 272, 677, 1349
BspANI GGCC 3 cut(s) 36, 1059, 1238
BspCNI CTCAG 2 cut(s) 1263, 1309
BspEI TCCGGA 1 cut(s) 1285
BspHI TCATGA 1 cut(s) 15
BspLI GGNNCC 2 cut(s) 339, 939
BspMI ACCTGC 1 cut(s) 728
BspPI GGATC 5 cut(s) 55, 332, 345, 380, 849
BspT107I GGYRCC 1 cut(s) 937
BspTNI GGTCTC 1 cut(s) 1428
BsrDI GCAATG 2 cut(s) 973, 1233
BsrFI RCCGGY 1 cut(s) 628
BsrGI TGTACA 1 cut(s) 775
BsrI ACTGG 4 cut(s) 576, 587, 858, 1398
BssAI RCCGGY 1 cut(s) 628
BssECI CCNNGG 3 cut(s) 150, 506, 622
BssMI GATC 6 cut(s) 18, 47, 337, 385, 854, 1125
BssT1I CCWWGG 1 cut(s) 150
Bst2UI CCWGG 3 cut(s) 507, 771, 1331
Bst4CI ACNGT 5 cut(s) 102, 738, 851, 1419, 1587
Bst6I CTCTTC 3 cut(s) 1005, 1249, 1569
BstAUI TGTACA 1 cut(s) 775
BstC8I GCNNGC 1 cut(s) 38
BstDEI CTNAG 2 cut(s) 1250, 1317
BstEII GGTNACC 1 cut(s) 806
BstF5I GGATG 5 cut(s) 115, 121, 483, 580, 1339
BstKTI GATC 6 cut(s) 21, 50, 340, 388, 857, 1128
BstMAI GTCTC 3 cut(s) 1428, 1502, 1551
BstMBI GATC 6 cut(s) 18, 47, 337, 385, 854, 1125
BstMWI GCNNNNNNNGC 2 cut(s) 683, 1567
BstNI CCWGG 3 cut(s) 507, 771, 1331
BstNSI RCATGY 1 cut(s) 315
BstPAI GACNNNNGTC 1 cut(s) 1420
BstPI GGTNACC 1 cut(s) 806
BstSCI CCNGG 3 cut(s) 505, 769, 1329
BstSFI CTRYAG 1 cut(s) 613
BstV1I GCAGC 2 cut(s) 332, 681
BstX2I RGATCY 2 cut(s) 337, 854
BstYI RGATCY 2 cut(s) 337, 854
BsuI GTATCC 1 cut(s) 148
BsuRI GGCC 3 cut(s) 36, 1059, 1238
BtsCI GGATG 5 cut(s) 115, 121, 483, 580, 1339
BtsIMutI CAGTG 4 cut(s) 569, 1405, 1424, 1592
BveI ACCTGC 1 cut(s) 728
Cac8I GCNNGC 1 cut(s) 38
CciI TCATGA 1 cut(s) 15
Cfr10I RCCGGY 1 cut(s) 628
Cfr13I GGNCC 6 cut(s) 35, 188, 296, 626, 1181, 1288
CsiI ACCWGGT 1 cut(s) 769
Csp6I GTAC 1 cut(s) 776
CspCI CAANNNNNGTGG 2 cut(s) 1446, 1481
CviAII CATG 7 cut(s) 16, 312, 342, 464, 704, 1376, 1629
CviQI GTAC 1 cut(s) 776
DdeI CTNAG 2 cut(s) 1250, 1317
DpnI GATC 6 cut(s) 20, 49, 339, 387, 856, 1127
DpnII GATC 6 cut(s) 18, 47, 337, 385, 854, 1125
DraI TTTAAA 1 cut(s) 994
DraIII CACNNNGTG 1 cut(s) 892
EaeI YGGCCR 1 cut(s) 1236
Eam1104I CTCTTC 3 cut(s) 1005, 1249, 1569
EarI CTCTTC 3 cut(s) 1005, 1249, 1569
Eco130I CCWWGG 1 cut(s) 150
Eco24I GRGCYC 1 cut(s) 369
Eco31I GGTCTC 1 cut(s) 1428
Eco47I GGWCC 5 cut(s) 188, 296, 626, 1181, 1288
Eco57I CTGAAG 1 cut(s) 1467
Eco88I CYCGRG 1 cut(s) 1121
Eco91I GGTNACC 1 cut(s) 806
EcoO109I RGGNCCY 1 cut(s) 296
EcoO65I GGTNACC 1 cut(s) 806
EcoRI GAATTC 2 cut(s) 1070, 1260
EcoRII CCWGG 3 cut(s) 505, 769, 1329
EcoT14I CCWWGG 1 cut(s) 150
EcoT38I GRGCYC 1 cut(s) 369
ErhI CCWWGG 1 cut(s) 150
Esp3I CGTCTC 1 cut(s) 1502
FaeI CATG 7 cut(s) 19, 315, 345, 467, 707, 1379, 1632
FalI AAGNNNNNCTT 2 cut(s) 1600, 1632
FaqI GGGAC 1 cut(s) 44
FatI CATG 7 cut(s) 15, 311, 341, 463, 703, 1375, 1628
FauI CCCGC 1 cut(s) 45
FauNDI CATATG 1 cut(s) 167
Fnu4HI GCNGC 3 cut(s) 272, 346, 670
FokI GGATG 5 cut(s) 122, 128, 470, 587, 1346
FriOI GRGCYC 1 cut(s) 369
Fsp4HI GCNGC 3 cut(s) 272, 346, 670
FspBI CTAG 4 cut(s) 531, 834, 1007, 1020
GluI GCNGC 3 cut(s) 272, 346, 670
HaeIII GGCC 3 cut(s) 36, 1059, 1238
HapII CCGG 2 cut(s) 629, 1286
Hin1II CATG 7 cut(s) 19, 315, 345, 467, 707, 1379, 1632
HincII GTYRAC 1 cut(s) 1090
HindII GTYRAC 1 cut(s) 1090
HinfI GANTC 5 cut(s) 480, 960, 1053, 1442, 1577
HpaII CCGG 2 cut(s) 629, 1286
HphI GGTGA 3 cut(s) 223, 818, 878
Hpy166II GTNNAC 6 cut(s) 214, 643, 776, 1090, 1181, 1204
Hpy188I TCNGA 7 cut(s) 414, 445, 623, 831, 1253, 1389, 1447
Hpy8I GTNNAC 6 cut(s) 214, 643, 776, 1090, 1181, 1204
HpyAV CCTTC 6 cut(s) 134, 287, 1070, 1288, 1442, 1490
HpyCH4III ACNGT 5 cut(s) 102, 738, 851, 1419, 1587
HpyCH4V TGCA 2 cut(s) 311, 1145
HpyF10VI GCNNNNNNNGC 2 cut(s) 683, 1567
HpyF3I CTNAG 2 cut(s) 1250, 1317
Hsp92II CATG 7 cut(s) 19, 315, 345, 467, 707, 1379, 1632
Kpn2I TCCGGA 1 cut(s) 1285
Kzo9I GATC 6 cut(s) 18, 47, 337, 385, 854, 1125
LmnI GCTCC 3 cut(s) 152, 286, 787
Lsp1109I GCAGC 2 cut(s) 332, 681
LweI GCATC 1 cut(s) 1035
MabI ACCWGGT 1 cut(s) 769
MaeI CTAG 4 cut(s) 531, 834, 1007, 1020
MaeIII GTNAC 4 cut(s) 565, 715, 732, 806
MalI GATC 6 cut(s) 20, 49, 339, 387, 856, 1127
MboI GATC 6 cut(s) 18, 47, 337, 385, 854, 1125
MboII GAAGA 6 cut(s) 1022, 1066, 1166, 1169, 1266, 1586
MfeI CAATTG 2 cut(s) 966, 1392
MflI RGATCY 2 cut(s) 337, 854
MhlI GDGCHC 1 cut(s) 369
MlsI TGGCCA 1 cut(s) 1238
MluCI AATT 9 cut(s) 59, 140, 436, 873, 966, 1070, 1138, 1260, 1392
MluNI TGGCCA 1 cut(s) 1238
MlyI GAGTC 2 cut(s) 474, 1586
MmeI TCCRAC 1 cut(s) 116
Mox20I TGGCCA 1 cut(s) 1238
MroI TCCGGA 1 cut(s) 1285
MroXI GAANNNNTTC 1 cut(s) 582
MscI TGGCCA 1 cut(s) 1238
MseI TTAA 7 cut(s) 63, 131, 263, 870, 993, 1037, 1380
Msp20I TGGCCA 1 cut(s) 1238
MspI CCGG 2 cut(s) 629, 1286
MspR9I CCNGG 3 cut(s) 507, 771, 1331
MunI CAATTG 2 cut(s) 966, 1392
MvaI CCWGG 3 cut(s) 507, 771, 1331
MwoI GCNNNNNNNGC 2 cut(s) 683, 1567
NdeI CATATG 1 cut(s) 167
NdeII GATC 6 cut(s) 18, 47, 337, 385, 854, 1125
NlaIII CATG 7 cut(s) 19, 315, 345, 467, 707, 1379, 1632
NlaIV GGNNCC 2 cut(s) 339, 939
NmuCI GTSAC 2 cut(s) 732, 806
NspI RCATGY 1 cut(s) 315
PaeR7I CTCGAG 1 cut(s) 1121
PagI TCATGA 1 cut(s) 15
PdmI GAANNNNTTC 1 cut(s) 582
PfeI GAWTC 3 cut(s) 960, 1053, 1442
PflFI GACNNNGTC 1 cut(s) 1587
PflMI CCANNNNNTGG 2 cut(s) 185, 755
PkrI GCNGC 3 cut(s) 273, 347, 671
PleI GAGTC 2 cut(s) 474, 1585
PpsI GAGTC 2 cut(s) 474, 1585
PpuMI RGGWCCY 1 cut(s) 296
PshAI GACNNNNGTC 1 cut(s) 1420
Psp5II RGGWCCY 1 cut(s) 296
Psp6I CCWGG 3 cut(s) 505, 769, 1329
PspEI GGTNACC 1 cut(s) 806
PspGI CCWGG 3 cut(s) 505, 769, 1329
PspN4I GGNNCC 2 cut(s) 339, 939
PspPI GGNCC 6 cut(s) 35, 188, 296, 626, 1181, 1288
PspPPI RGGWCCY 1 cut(s) 296
PsrI GAACNNNNNNTAC 2 cut(s) 424, 456
PsuI RGATCY 2 cut(s) 337, 854
PsyI GACNNNGTC 1 cut(s) 1587
RsaI GTAC 1 cut(s) 777
RsaNI GTAC 1 cut(s) 776
SaqAI TTAA 7 cut(s) 63, 131, 263, 870, 993, 1037, 1380
SatI GCNGC 3 cut(s) 272, 346, 670
Sau3AI GATC 6 cut(s) 18, 47, 337, 385, 854, 1125
Sau96I GGNCC 6 cut(s) 35, 188, 296, 626, 1181, 1288
SchI GAGTC 2 cut(s) 474, 1586
ScrFI CCNGG 3 cut(s) 507, 771, 1331
SduI GDGCHC 1 cut(s) 369
SexAI ACCWGGT 1 cut(s) 769
SfaNI GCATC 1 cut(s) 1035
SfcI CTRYAG 1 cut(s) 613
Sfr274I CTCGAG 1 cut(s) 1121
SinI GGWCC 5 cut(s) 188, 296, 626, 1181, 1288
SlaI CTCGAG 1 cut(s) 1121
SmlI CTYRAG 2 cut(s) 944, 1121
SmoI CTYRAG 2 cut(s) 944, 1121
Sse9I AATT 9 cut(s) 59, 140, 436, 873, 966, 1070, 1138, 1260, 1392
SsiI CCGC 4 cut(s) 38, 272, 677, 1349
SspI AATATT 1 cut(s) 1150
SspMI CTAG 4 cut(s) 531, 834, 1007, 1020
StyD4I CCNGG 3 cut(s) 505, 769, 1329
StyI CCWWGG 1 cut(s) 150
TaaI ACNGT 5 cut(s) 102, 738, 851, 1419, 1587
TaqI TCGA 9 cut(s) 21, 88, 375, 597, 766, 915, 1029, 1122, 1128
TasI AATT 9 cut(s) 59, 140, 436, 873, 966, 1070, 1138, 1260, 1392
TatI WGTACW 1 cut(s) 775
TauI GCSGC 1 cut(s) 274
TfiI GAWTC 3 cut(s) 960, 1053, 1442
Tru1I TTAA 7 cut(s) 63, 131, 263, 870, 993, 1037, 1380
Tru9I TTAA 7 cut(s) 63, 131, 263, 870, 993, 1037, 1380
TscAI CASTG 4 cut(s) 576, 1405, 1424, 1592
TseFI GTSAC 2 cut(s) 732, 806
TseI GCWGC 2 cut(s) 345, 669
Tsp45I GTSAC 2 cut(s) 732, 806
TspGWI ACGGA 3 cut(s) 117, 350, 1526
TspRI CASTG 4 cut(s) 576, 1405, 1424, 1592
Tth111I GACNNNGTC 1 cut(s) 1587
Van91I CCANNNNNTGG 2 cut(s) 185, 755
VpaK11BI GGWCC 5 cut(s) 188, 296, 626, 1181, 1288
XapI RAATTY 4 cut(s) 59, 140, 1070, 1260
XbaI TCTAGA 1 cut(s) 530
XceI RCATGY 1 cut(s) 315
XhoI CTCGAG 1 cut(s) 1121
XmnI GAANNNNTTC 1 cut(s) 582
XspI CTAG 4 cut(s) 531, 834, 1007, 1020
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.