Rroxscaffold_5G00341800

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0003702
Physical Location & Seq
Reverse (-)
10401669 .. 10407142
5474 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00341800.1

Sequence Viewer

Length: 519 bp
ATGGCGATGTACAATCTCTATCACACTTCGGAAGGTGTTGATTGTGATGAGAAGCACATCATTTTGCTTCAACATGGCATAGTAGAGCCAAAGCGTGGCACTAAACAGTGCTACCAGATACAGAACCGATTGGAAACCCTTAGTCGATTTCTTTCTGTAAATCCTGTAAAACGATGCCAAATGCAAATGCCCAAGGATGTTGACTGTCTGCTACTGCGCCCATCAAAGGGTGAACAAACTCGGTTTAATGTAACGAAAACTTTGAGAAGATTGAGATCAAAGAAGAGGAAGAAGAAGAAGATAGAACTATTTAAAAGTTTCAGTGCTAAGAAACCCGAACATCCTTGCCATCACCGGATACCCATCGACGGCGAGCCCTACGTGCATGGCCAATTCGACTCCGTCACATTCACTTCGACAATGGCTCAAGCCGGCCGCAGCTCCCACAGTGGTTCCTACCCTCGGTGGTTGACTCATTACCGCGAAAGCACAATGCGATCGGAGGAATTTCTTTCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

172

Amino Acids

20.15

Weight (kDa)

9.81

Isoelectric Point (pI)

60.11

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 95
AccII CGCG 1 cut(s) 483
AciI CCGC 2 cut(s) 436, 481
AcoI YGGCCR 2 cut(s) 388, 433
AcsI RAATTY 1 cut(s) 506
AfaI GTAC 1 cut(s) 11
AfiI CCNNNNNNNGG 5 cut(s) 95, 226, 227, 368, 462
AgsI TTSAA 1 cut(s) 71
AluBI AGCT 1 cut(s) 441
AluI AGCT 1 cut(s) 441
AoxI GGCC 2 cut(s) 388, 433
ApeKI GCWGC 1 cut(s) 438
ApoI RAATTY 1 cut(s) 506
AspLEI GCGC 1 cut(s) 219
AsuHPI GGTGA 2 cut(s) 242, 344
BalI TGGCCA 1 cut(s) 390
BanII GRGCYC 1 cut(s) 378
BbvI GCAGC 1 cut(s) 450
BccI CCATC 3 cut(s) 229, 357, 371
BceAI ACGGC 1 cut(s) 385
BciVI GTATCC 1 cut(s) 351
BfuI GTATCC 1 cut(s) 351
BisI GCNGC 2 cut(s) 436, 439
BlsI GCNGC 2 cut(s) 437, 440
BmiI GGNNCC 1 cut(s) 454
BmsI GCATC 1 cut(s) 164
BpuEI CTTGAG 1 cut(s) 411
BsaAI YACGTR 1 cut(s) 382
BsaBI GATNNNNATC 2 cut(s) 274, 362
BsaJI CCNNGG 2 cut(s) 192, 461
BsaWI WCCGGW 1 cut(s) 354
Bsc4I CCNNNNNNNGG 5 cut(s) 95, 226, 227, 368, 462
Bse118I RCCGGY 1 cut(s) 431
Bse8I GATNNNNATC 2 cut(s) 274, 362
BseDI CCNNGG 2 cut(s) 192, 461
BseGI GGATG 2 cut(s) 202, 340
BseJI GATNNNNATC 2 cut(s) 274, 362
BseLI CCNNNNNNNGG 5 cut(s) 95, 226, 227, 368, 462
BseX3I CGGCCG 1 cut(s) 433
BseXI GCAGC 1 cut(s) 450
Bsh1236I CGCG 1 cut(s) 483
Bsh1285I CGRYCG 2 cut(s) 436, 500
BshFI GGCC 2 cut(s) 390, 435
BsiEI CGRYCG 2 cut(s) 436, 500
BsiSI CCGG 2 cut(s) 355, 432
BslI CCNNNNNNNGG 5 cut(s) 95, 226, 227, 368, 462
BsnI GGCC 2 cut(s) 390, 435
Bsp1286I GDGCHC 1 cut(s) 378
Bsp1407I TGTACA 1 cut(s) 9
Bsp143I GATC 2 cut(s) 275, 497
BspACI CCGC 2 cut(s) 436, 481
BspANI GGCC 2 cut(s) 390, 435
BspFNI CGCG 1 cut(s) 483
BspLI GGNNCC 1 cut(s) 454
BsrFI RCCGGY 1 cut(s) 431
BsrGI TGTACA 1 cut(s) 9
BssAI RCCGGY 1 cut(s) 431
BssECI CCNNGG 2 cut(s) 192, 461
BssMI GATC 2 cut(s) 275, 497
BssT1I CCWWGG 1 cut(s) 192
Bst4CI ACNGT 3 cut(s) 108, 206, 449
Bst6I CTCTTC 1 cut(s) 278
BstAUI TGTACA 1 cut(s) 9
BstBAI YACGTR 1 cut(s) 382
BstC8I GCNNGC 2 cut(s) 374, 433
BstDEI CTNAG 2 cut(s) 140, 327
BstF5I GGATG 2 cut(s) 202, 340
BstFNI CGCG 1 cut(s) 483
BstHHI GCGC 1 cut(s) 219
BstKTI GATC 2 cut(s) 278, 500
BstMBI GATC 2 cut(s) 275, 497
BstMCI CGRYCG 2 cut(s) 436, 500
BstMWI GCNNNNNNNGC 1 cut(s) 382
BstUI CGCG 1 cut(s) 483
BstV1I GCAGC 1 cut(s) 450
BstZI CGGCCG 1 cut(s) 433
BsuI GTATCC 1 cut(s) 351
BsuRI GGCC 2 cut(s) 390, 435
BtgZI GCGATG 1 cut(s) 20
BtsCI GGATG 2 cut(s) 202, 340
BtsIMutI CAGTG 3 cut(s) 113, 328, 454
Cac8I GCNNGC 2 cut(s) 374, 433
CfoI GCGC 1 cut(s) 219
Cfr10I RCCGGY 1 cut(s) 431
Csp6I GTAC 1 cut(s) 10
CviAII CATG 2 cut(s) 74, 386
CviJI RGCY 7 cut(s) 88, 376, 390, 425, 431, 435, 441
CviKI_1 RGCY 7 cut(s) 88, 376, 390, 425, 431, 435, 441
CviQI GTAC 1 cut(s) 10
DdeI CTNAG 2 cut(s) 140, 327
DpnI GATC 2 cut(s) 277, 499
DpnII GATC 2 cut(s) 275, 497
DraI TTTAAA 1 cut(s) 313
EaeI YGGCCR 2 cut(s) 388, 433
EagI CGGCCG 1 cut(s) 433
Eam1104I CTCTTC 1 cut(s) 278
EarI CTCTTC 1 cut(s) 278
EclXI CGGCCG 1 cut(s) 433
Eco130I CCWWGG 1 cut(s) 192
Eco24I GRGCYC 1 cut(s) 378
Eco52I CGGCCG 1 cut(s) 433
EcoT14I CCWWGG 1 cut(s) 192
EcoT38I GRGCYC 1 cut(s) 378
ErhI CCWWGG 1 cut(s) 192
FaeI CATG 2 cut(s) 77, 389
FaiI YATR 4 cut(s) 75, 80, 387, 517
FatI CATG 2 cut(s) 73, 385
Fnu4HI GCNGC 2 cut(s) 436, 439
FokI GGATG 2 cut(s) 209, 327
FriOI GRGCYC 1 cut(s) 378
Fsp4HI GCNGC 2 cut(s) 436, 439
GlaI GCGC 1 cut(s) 218
GluI GCNGC 2 cut(s) 436, 439
HaeIII GGCC 2 cut(s) 390, 435
HapII CCGG 2 cut(s) 355, 432
HhaI GCGC 1 cut(s) 219
Hin1II CATG 2 cut(s) 77, 389
Hin6I GCGC 1 cut(s) 217
HinP1I GCGC 1 cut(s) 217
HincII GTYRAC 2 cut(s) 202, 471
HindII GTYRAC 2 cut(s) 202, 471
HinfI GANTC 2 cut(s) 398, 472
HpaII CCGG 2 cut(s) 355, 432
HphI GGTGA 2 cut(s) 242, 344
Hpy166II GTNNAC 3 cut(s) 202, 233, 471
Hpy188I TCNGA 2 cut(s) 31, 502
Hpy8I GTNNAC 3 cut(s) 202, 233, 471
Hpy99I CGWCG 1 cut(s) 371
HpyAV CCTTC 1 cut(s) 26
HpyCH4III ACNGT 3 cut(s) 108, 206, 449
HpyCH4IV ACGT 1 cut(s) 381
HpyCH4V TGCA 2 cut(s) 184, 385
HpyF10VI GCNNNNNNNGC 1 cut(s) 382
HpyF3I CTNAG 2 cut(s) 140, 327
HpySE526I ACGT 1 cut(s) 381
Hsp92II CATG 2 cut(s) 77, 389
HspAI GCGC 1 cut(s) 217
KroI GCCGGC 1 cut(s) 431
KroNI GCCGGC 1 cut(s) 433
Kzo9I GATC 2 cut(s) 275, 497
LmnI GCTCC 1 cut(s) 446
LpnPI CCDG 4 cut(s) 128, 177, 368, 445
Lsp1109I GCAGC 1 cut(s) 450
LweI GCATC 1 cut(s) 164
MaeII ACGT 1 cut(s) 381
MaeIII GTNAC 2 cut(s) 250, 403
MalI GATC 2 cut(s) 277, 499
MboI GATC 2 cut(s) 275, 497
MboII GAAGA 6 cut(s) 279, 295, 301, 304, 307, 310
MhlI GDGCHC 1 cut(s) 378
MlsI TGGCCA 1 cut(s) 390
MluCI AATT 2 cut(s) 392, 506
MluNI TGGCCA 1 cut(s) 390
MlyI GAGTC 2 cut(s) 392, 466
MnlI CCTC 3 cut(s) 279, 471, 496
Mox20I TGGCCA 1 cut(s) 390
MroNI GCCGGC 1 cut(s) 431
MscI TGGCCA 1 cut(s) 390
MseI TTAA 2 cut(s) 246, 312
Msp20I TGGCCA 1 cut(s) 390
MspI CCGG 2 cut(s) 355, 432
MvnI CGCG 1 cut(s) 483
MwoI GCNNNNNNNGC 1 cut(s) 382
NaeI GCCGGC 1 cut(s) 433
NdeII GATC 2 cut(s) 275, 497
NgoMIV GCCGGC 1 cut(s) 431
NlaIII CATG 2 cut(s) 77, 389
NlaIV GGNNCC 1 cut(s) 454
NmuCI GTSAC 1 cut(s) 403
PdiI GCCGGC 1 cut(s) 433
PflFI GACNNNGTC 1 cut(s) 401
PflMI CCANNNNNTGG 1 cut(s) 95
PkrI GCNGC 2 cut(s) 437, 440
Ple19I CGATCG 1 cut(s) 500
PleI GAGTC 2 cut(s) 392, 466
PpsI GAGTC 2 cut(s) 392, 466
Ppu21I YACGTR 1 cut(s) 382
PspN4I GGNNCC 1 cut(s) 454
PsyI GACNNNGTC 1 cut(s) 401
PvuI CGATCG 1 cut(s) 500
RsaI GTAC 1 cut(s) 11
RsaNI GTAC 1 cut(s) 10
SaqAI TTAA 2 cut(s) 246, 312
SatI GCNGC 2 cut(s) 436, 439
Sau3AI GATC 2 cut(s) 275, 497
SchI GAGTC 2 cut(s) 392, 466
SduI GDGCHC 1 cut(s) 378
SetI ASST 3 cut(s) 37, 384, 443
SfaNI GCATC 1 cut(s) 164
SmlI CTYRAG 1 cut(s) 426
SmoI CTYRAG 1 cut(s) 426
Sse9I AATT 2 cut(s) 392, 506
SsiI CCGC 2 cut(s) 436, 481
StyI CCWWGG 1 cut(s) 192
TaaI ACNGT 3 cut(s) 108, 206, 449
TaiI ACGT 1 cut(s) 384
TaqI TCGA 4 cut(s) 145, 366, 396, 416
TasI AATT 2 cut(s) 392, 506
TatI WGTACW 1 cut(s) 9
TauI GCSGC 1 cut(s) 438
Tru1I TTAA 2 cut(s) 246, 312
Tru9I TTAA 2 cut(s) 246, 312
TscAI CASTG 3 cut(s) 113, 328, 454
TseFI GTSAC 1 cut(s) 403
TseI GCWGC 1 cut(s) 438
Tsp45I GTSAC 1 cut(s) 403
TspDTI ATGAA 1 cut(s) 504
TspGWI ACGGA 1 cut(s) 391
TspRI CASTG 3 cut(s) 113, 328, 454
Tth111I GACNNNGTC 1 cut(s) 401
Van91I CCANNNNNTGG 1 cut(s) 95
XapI RAATTY 1 cut(s) 506
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.