Serine threonine-protein kinase
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AciI | CCGC | 1 cut(s) | 242 |
| AclWI | GGATC | 2 cut(s) | 210, 634 |
| AcoI | YGGCCR | 1 cut(s) | 243 |
| AcsI | RAATTY | 1 cut(s) | 553 |
| AcuI | CTGAAG | 2 cut(s) | 408, 918 |
| AfaI | GTAC | 2 cut(s) | 680, 1141 |
| AfiI | CCNNNNNNNGG | 5 cut(s) | 98, 166, 312, 545, 591 |
| AgsI | TTSAA | 3 cut(s) | 7, 479, 952 |
| AjnI | CCWGG | 2 cut(s) | 118, 856 |
| AluBI | AGCT | 9 cut(s) | 173, 475, 482, 500, 620, 926, 932, 940, 1064 |
| AluI | AGCT | 9 cut(s) | 173, 475, 482, 500, 620, 926, 932, 940, 1064 |
| Alw21I | GWGCWC | 1 cut(s) | 907 |
| Alw26I | GTCTC | 2 cut(s) | 706, 765 |
| AlwI | GGATC | 2 cut(s) | 210, 634 |
| AoxI | GGCC | 7 cut(s) | 89, 116, 243, 314, 535, 582, 963 |
| ApaI | GGGCCC | 1 cut(s) | 967 |
| ApeKI | GCWGC | 7 cut(s) | 170, 497, 500, 620, 926, 929, 1193 |
| ApoI | RAATTY | 1 cut(s) | 553 |
| AseI | ATTAAT | 1 cut(s) | 251 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 956 |
| AspS9I | GGNCC | 5 cut(s) | 50, 536, 963, 964, 1103 |
| AsuHPI | GGTGA | 2 cut(s) | 134, 550 |
| AsuNHI | GCTAGC | 1 cut(s) | 616 |
| AvaII | GGWCC | 2 cut(s) | 50, 1103 |
| AxyI | CCTNAGG | 1 cut(s) | 1109 |
| BaeGI | GKGCMC | 1 cut(s) | 967 |
| BanII | GRGCYC | 1 cut(s) | 967 |
| Bbv12I | GWGCWC | 1 cut(s) | 907 |
| BbvI | GCAGC | 7 cut(s) | 182, 487, 509, 607, 913, 941, 1205 |
| BccI | CCATC | 4 cut(s) | 61, 451, 500, 1163 |
| BciT130I | CCWGG | 2 cut(s) | 120, 858 |
| BclI | TGATCA | 2 cut(s) | 228, 708 |
| BcoDI | GTCTC | 2 cut(s) | 706, 765 |
| BfaI | CTAG | 3 cut(s) | 617, 893, 1029 |
| BfmI | CTRYAG | 4 cut(s) | 323, 501, 804, 927 |
| BisI | GCNGC | 8 cut(s) | 171, 243, 498, 501, 621, 927, 930, 1194 |
| BlsI | GCNGC | 8 cut(s) | 172, 244, 499, 502, 622, 928, 931, 1195 |
| Bme1390I | CCNGG | 2 cut(s) | 120, 858 |
| Bme18I | GGWCC | 2 cut(s) | 50, 1103 |
| BmgT120I | GGNCC | 5 cut(s) | 50, 536, 963, 964, 1103 |
| BmiI | GGNNCC | 4 cut(s) | 538, 964, 965, 1105 |
| BmrFI | CCNGG | 2 cut(s) | 120, 858 |
| BmrI | ACTGGG | 1 cut(s) | 919 |
| BmsI | GCATC | 2 cut(s) | 334, 555 |
| BmtI | GCTAGC | 1 cut(s) | 620 |
| BmuI | ACTGGG | 1 cut(s) | 919 |
| BpuEI | CTTGAG | 2 cut(s) | 218, 235 |
| BsaJI | CCNNGG | 3 cut(s) | 311, 727, 857 |
| Bsc4I | CCNNNNNNNGG | 5 cut(s) | 98, 166, 312, 545, 591 |
| Bse1I | ACTGG | 2 cut(s) | 791, 914 |
| Bse21I | CCTNAGG | 1 cut(s) | 1109 |
| Bse3DI | GCAATG | 1 cut(s) | 66 |
| BseBI | CCWGG | 2 cut(s) | 120, 858 |
| BseDI | CCNNGG | 3 cut(s) | 311, 727, 857 |
| BseGI | GGATG | 1 cut(s) | 1120 |
| BseLI | CCNNNNNNNGG | 5 cut(s) | 98, 166, 312, 545, 591 |
| BseMI | GCAATG | 1 cut(s) | 66 |
| BseMII | CTCAG | 6 cut(s) | 366, 485, 747, 1029, 1084, 1123 |
| BseNI | ACTGG | 2 cut(s) | 791, 914 |
| BseRI | GAGGAG | 3 cut(s) | 541, 561, 1243 |
| BseSI | GKGCMC | 1 cut(s) | 967 |
| BseXI | GCAGC | 7 cut(s) | 182, 487, 509, 607, 913, 941, 1205 |
| BshFI | GGCC | 7 cut(s) | 91, 118, 245, 316, 537, 584, 965 |
| BsiHKAI | GWGCWC | 1 cut(s) | 907 |
| BslI | CCNNNNNNNGG | 5 cut(s) | 98, 166, 312, 545, 591 |
| BsmAI | GTCTC | 2 cut(s) | 706, 765 |
| BsnI | GGCC | 7 cut(s) | 91, 118, 245, 316, 537, 584, 965 |
| Bsp120I | GGGCCC | 1 cut(s) | 963 |
| Bsp1286I | GDGCHC | 2 cut(s) | 907, 967 |
| Bsp143I | GATC | 4 cut(s) | 202, 228, 626, 708 |
| BspACI | CCGC | 1 cut(s) | 242 |
| BspANI | GGCC | 7 cut(s) | 91, 118, 245, 316, 537, 584, 965 |
| BspCNI | CTCAG | 6 cut(s) | 367, 484, 748, 1028, 1083, 1122 |
| BspLI | GGNNCC | 4 cut(s) | 538, 964, 965, 1105 |
| BspMAI | CTGCAG | 3 cut(s) | 327, 505, 931 |
| BspOI | GCTAGC | 1 cut(s) | 620 |
| BspPI | GGATC | 2 cut(s) | 210, 634 |
| BsrDI | GCAATG | 1 cut(s) | 66 |
| BsrI | ACTGG | 2 cut(s) | 791, 914 |
| BssECI | CCNNGG | 3 cut(s) | 311, 727, 857 |
| BssMI | GATC | 4 cut(s) | 202, 228, 626, 708 |
| BssT1I | CCWWGG | 1 cut(s) | 311 |
| Bst2UI | CCWGG | 2 cut(s) | 120, 858 |
| Bst4CI | ACNGT | 3 cut(s) | 489, 640, 880 |
| Bst6I | CTCTTC | 2 cut(s) | 526, 947 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 995 |
| BstC8I | GCNNGC | 4 cut(s) | 381, 570, 618, 924 |
| BstDEI | CTNAG | 6 cut(s) | 375, 471, 756, 1015, 1070, 1109 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 96 |
| BstF5I | GGATG | 1 cut(s) | 1120 |
| BstKTI | GATC | 4 cut(s) | 205, 231, 629, 711 |
| BstMAI | GTCTC | 2 cut(s) | 706, 765 |
| BstMBI | GATC | 4 cut(s) | 202, 228, 626, 708 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 322, 509, 911, 995 |
| BstNI | CCWGG | 2 cut(s) | 120, 858 |
| BstSCI | CCNGG | 2 cut(s) | 118, 856 |
| BstSFI | CTRYAG | 4 cut(s) | 323, 501, 804, 927 |
| BstSLI | GKGCMC | 1 cut(s) | 967 |
| BstV1I | GCAGC | 7 cut(s) | 182, 487, 509, 607, 913, 941, 1205 |
| BstX2I | RGATCY | 2 cut(s) | 202, 626 |
| BstYI | RGATCY | 2 cut(s) | 202, 626 |
| Bsu36I | CCTNAGG | 1 cut(s) | 1109 |
| BsuRI | GGCC | 7 cut(s) | 91, 118, 245, 316, 537, 584, 965 |
| BtsCI | GGATG | 1 cut(s) | 1120 |
| BtsI | GCAGTG | 1 cut(s) | 519 |
| BtsIMutI | CAGTG | 5 cut(s) | 519, 681, 798, 876, 907 |
| Cac8I | GCNNGC | 4 cut(s) | 381, 570, 618, 924 |
| Cfr13I | GGNCC | 5 cut(s) | 50, 536, 963, 964, 1103 |
| CseI | GACGC | 1 cut(s) | 12 |
| Csp6I | GTAC | 2 cut(s) | 679, 1140 |
| CviAII | CATG | 4 cut(s) | 598, 706, 820, 1207 |
| CviQI | GTAC | 2 cut(s) | 679, 1140 |
| DdeI | CTNAG | 6 cut(s) | 375, 471, 756, 1015, 1070, 1109 |
| DpnI | GATC | 4 cut(s) | 204, 230, 628, 710 |
| DpnII | GATC | 4 cut(s) | 202, 228, 626, 708 |
| EaeI | YGGCCR | 1 cut(s) | 243 |
| Eam1104I | CTCTTC | 2 cut(s) | 526, 947 |
| EarI | CTCTTC | 2 cut(s) | 526, 947 |
| Eco130I | CCWWGG | 1 cut(s) | 311 |
| Eco147I | AGGCCT | 1 cut(s) | 91 |
| Eco24I | GRGCYC | 1 cut(s) | 967 |
| Eco47I | GGWCC | 2 cut(s) | 50, 1103 |
| Eco57I | CTGAAG | 2 cut(s) | 408, 918 |
| Eco81I | CCTNAGG | 1 cut(s) | 1109 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 96 |
| EcoO109I | RGGNCCY | 3 cut(s) | 536, 963, 964 |
| EcoRII | CCWGG | 2 cut(s) | 118, 856 |
| EcoT14I | CCWWGG | 1 cut(s) | 311 |
| EcoT22I | ATGCAT | 1 cut(s) | 1187 |
| EcoT38I | GRGCYC | 1 cut(s) | 967 |
| ErhI | CCWWGG | 1 cut(s) | 311 |
| FaeI | CATG | 4 cut(s) | 601, 709, 823, 1210 |
| FatI | CATG | 4 cut(s) | 597, 705, 819, 1206 |
| FbaI | TGATCA | 2 cut(s) | 228, 708 |
| Fnu4HI | GCNGC | 8 cut(s) | 171, 243, 498, 501, 621, 927, 930, 1194 |
| FokI | GGATG | 1 cut(s) | 1127 |
| FriOI | GRGCYC | 1 cut(s) | 967 |
| Fsp4HI | GCNGC | 8 cut(s) | 171, 243, 498, 501, 621, 927, 930, 1194 |
| FspBI | CTAG | 3 cut(s) | 617, 893, 1029 |
| GluI | GCNGC | 8 cut(s) | 171, 243, 498, 501, 621, 927, 930, 1194 |
| HaeIII | GGCC | 7 cut(s) | 91, 118, 245, 316, 537, 584, 965 |
| HgaI | GACGC | 1 cut(s) | 12 |
| Hin1II | CATG | 4 cut(s) | 601, 709, 823, 1210 |
| HinfI | GANTC | 5 cut(s) | 135, 434, 465, 1167, 1255 |
| HphI | GGTGA | 2 cut(s) | 134, 550 |
| Hpy166II | GTNNAC | 4 cut(s) | 144, 681, 876, 1237 |
| Hpy188III | TCNNGA | 1 cut(s) | 523 |
| Hpy8I | GTNNAC | 4 cut(s) | 144, 681, 876, 1237 |
| Hpy99I | CGWCG | 1 cut(s) | 28 |
| HpyAV | CCTTC | 4 cut(s) | 344, 356, 412, 449 |
| HpyCH4III | ACNGT | 3 cut(s) | 489, 640, 880 |
| HpyCH4IV | ACGT | 2 cut(s) | 140, 733 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 322, 509, 911, 995 |
| HpyF3I | CTNAG | 6 cut(s) | 375, 471, 756, 1015, 1070, 1109 |
| HpySE526I | ACGT | 2 cut(s) | 140, 733 |
| Hsp92II | CATG | 4 cut(s) | 601, 709, 823, 1210 |
| Ksp22I | TGATCA | 2 cut(s) | 228, 708 |
| Kzo9I | GATC | 4 cut(s) | 202, 228, 626, 708 |
| LmnI | GCTCC | 1 cut(s) | 509 |
| Lsp1109I | GCAGC | 7 cut(s) | 182, 487, 509, 607, 913, 941, 1205 |
| LweI | GCATC | 2 cut(s) | 334, 555 |
| MaeI | CTAG | 3 cut(s) | 617, 893, 1029 |
| MaeII | ACGT | 2 cut(s) | 140, 733 |
| MaeIII | GTNAC | 3 cut(s) | 101, 122, 669 |
| MalI | GATC | 4 cut(s) | 204, 230, 628, 710 |
| MboI | GATC | 4 cut(s) | 202, 228, 626, 708 |
| MboII | GAAGA | 6 cut(s) | 292, 368, 371, 422, 543, 964 |
| MfeI | CAATTG | 1 cut(s) | 1264 |
| MflI | RGATCY | 2 cut(s) | 202, 626 |
| MhlI | GDGCHC | 2 cut(s) | 907, 967 |
| MluCI | AATT | 6 cut(s) | 339, 553, 649, 915, 973, 1264 |
| MmeI | TCCRAC | 1 cut(s) | 28 |
| Mph1103I | ATGCAT | 1 cut(s) | 1187 |
| MroXI | GAANNNNTTC | 1 cut(s) | 956 |
| MseI | TTAA | 2 cut(s) | 251, 1202 |
| MslI | CAYNNNNRTG | 1 cut(s) | 563 |
| MspA1I | CMGCKG | 2 cut(s) | 500, 926 |
| MspR9I | CCNGG | 2 cut(s) | 120, 858 |
| MunI | CAATTG | 1 cut(s) | 1264 |
| MvaI | CCWGG | 2 cut(s) | 120, 858 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 322, 509, 911, 995 |
| NdeII | GATC | 4 cut(s) | 202, 228, 626, 708 |
| NheI | GCTAGC | 1 cut(s) | 616 |
| NlaIII | CATG | 4 cut(s) | 601, 709, 823, 1210 |
| NlaIV | GGNNCC | 4 cut(s) | 538, 964, 965, 1105 |
| NmuCI | GTSAC | 3 cut(s) | 101, 122, 669 |
| NsiI | ATGCAT | 1 cut(s) | 1187 |
| PceI | AGGCCT | 1 cut(s) | 91 |
| PdmI | GAANNNNTTC | 1 cut(s) | 956 |
| PfeI | GAWTC | 5 cut(s) | 135, 434, 465, 1167, 1255 |
| PkrI | GCNGC | 8 cut(s) | 172, 244, 499, 502, 622, 928, 931, 1195 |
| PshBI | ATTAAT | 1 cut(s) | 251 |
| Psp6I | CCWGG | 2 cut(s) | 118, 856 |
| PspGI | CCWGG | 2 cut(s) | 118, 856 |
| PspN4I | GGNNCC | 4 cut(s) | 538, 964, 965, 1105 |
| PspOMI | GGGCCC | 1 cut(s) | 963 |
| PspPI | GGNCC | 5 cut(s) | 50, 536, 963, 964, 1103 |
| PstI | CTGCAG | 3 cut(s) | 327, 505, 931 |
| PsuI | RGATCY | 2 cut(s) | 202, 626 |
| PvuII | CAGCTG | 2 cut(s) | 500, 926 |
| RsaI | GTAC | 2 cut(s) | 680, 1141 |
| RsaNI | GTAC | 2 cut(s) | 679, 1140 |
| RseI | CAYNNNNRTG | 1 cut(s) | 563 |
| SaqAI | TTAA | 2 cut(s) | 251, 1202 |
| SatI | GCNGC | 8 cut(s) | 171, 243, 498, 501, 621, 927, 930, 1194 |
| Sau3AI | GATC | 4 cut(s) | 202, 228, 626, 708 |
| Sau96I | GGNCC | 5 cut(s) | 50, 536, 963, 964, 1103 |
| ScrFI | CCNGG | 2 cut(s) | 120, 858 |
| SduI | GDGCHC | 2 cut(s) | 907, 967 |
| SfaNI | GCATC | 2 cut(s) | 334, 555 |
| SfcI | CTRYAG | 4 cut(s) | 323, 501, 804, 927 |
| SinI | GGWCC | 2 cut(s) | 50, 1103 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 563 |
| SmlI | CTYRAG | 2 cut(s) | 197, 214 |
| SmoI | CTYRAG | 2 cut(s) | 197, 214 |
| Sse9I | AATT | 6 cut(s) | 339, 553, 649, 915, 973, 1264 |
| SseBI | AGGCCT | 1 cut(s) | 91 |
| SsiI | CCGC | 1 cut(s) | 242 |
| SspMI | CTAG | 3 cut(s) | 617, 893, 1029 |
| StuI | AGGCCT | 1 cut(s) | 91 |
| StyD4I | CCNGG | 2 cut(s) | 118, 856 |
| StyI | CCWWGG | 1 cut(s) | 311 |
| TaaI | ACNGT | 3 cut(s) | 489, 640, 880 |
| TaiI | ACGT | 2 cut(s) | 143, 736 |
| TaqI | TCGA | 2 cut(s) | 645, 1222 |
| TasI | AATT | 6 cut(s) | 339, 553, 649, 915, 973, 1264 |
| TauI | GCSGC | 1 cut(s) | 245 |
| TfiI | GAWTC | 5 cut(s) | 135, 434, 465, 1167, 1255 |
| Tru1I | TTAA | 2 cut(s) | 251, 1202 |
| Tru9I | TTAA | 2 cut(s) | 251, 1202 |
| TscAI | CASTG | 5 cut(s) | 519, 688, 798, 883, 914 |
| TseFI | GTSAC | 3 cut(s) | 101, 122, 669 |
| TseI | GCWGC | 7 cut(s) | 170, 497, 500, 620, 926, 929, 1193 |
| Tsp45I | GTSAC | 3 cut(s) | 101, 122, 669 |
| TspDTI | ATGAA | 1 cut(s) | 70 |
| TspRI | CASTG | 5 cut(s) | 519, 688, 798, 883, 914 |
| VpaK11BI | GGWCC | 2 cut(s) | 50, 1103 |
| VspI | ATTAAT | 1 cut(s) | 251 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 96 |
| XapI | RAATTY | 1 cut(s) | 553 |
| XmnI | GAANNNNTTC | 1 cut(s) | 956 |
| XspI | CTAG | 3 cut(s) | 617, 893, 1029 |
| Zsp2I | ATGCAT | 1 cut(s) | 1187 |