MD02G1306900.v1.1

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0000055
Physical Location & Seq
Reverse (-)
36126086 .. 36132972
6887 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1306900.v1.1.491

Sequence Viewer

Length: 3411 bp
ATGGATGCCTCGACGTCCTCGTCTTCCACCTTAAAACGTTGGAATTACGACGTGTACTTGAGCTTTCGAGGTGAATACACACGCCCGAACTTCACGGGTTTCACGAACCACCTCTGCTACGCGTTGAAGCACGCCAAAGTCAACTTCTTTGTCGACGAAGGCAGTCGAAGTGGATTGGAGTATCTGAGAAACGATCTGAATCAGGCAATCCAAGGGTCTAAAGTCGCTGTCATCGTCTTCTCGGAGGACTATGCGGAATCAATATTTTGTCTTGATGATCTGGCGAAGATCATGGAGTGCAGAAGAACACTGGGTCAAATGGTTATTCCAATATTCTGTGATGTCCAACCTTCAGAAGTCCGGACACAGACCGGTACTTTTGCAACAGCATTTCTGGACCACGAATGGCAGTTCTATGACGCCAAAGAAAGGTTACATCTATGGAGAGACGCTCTTACTGAAGCTGCAAATTTGTCTGGTTTTTATGTTAGAAACACCATTTGGGACGAAGGACAAAAGTATATCGGGGAAATTGTCACAGAGATCATCAGAGTCTTGCACCGCATACCATGCTTAGCCACCAATCCAGTTGGAATAGATTCTCGGGTGCAAGAAATCAGTAATTATTTAGATGTTGGAGGATCAAATGATGTTCGCATAATTGGAATTTGGGGTATGGGTGGAGTGGGTAAAACGACGGTTGCCATAGCCATTTTCAACAAATATCAGAATTTGTTTGATGGTAAAAGTTTTCTTGCTAGTGTGAGAGAAGAGAAACTGGTGAAGTCGCAATACACACTTCTTTCTGATGTATTGAGATCGGGAAACATAAAGGTTAGTAGAATTGATGAAGGGACCGAGGATATAAAAAGAAGACTTGGCAACCAAATGGTACTTGTCATAGTTGATGAGGTAGATAGCGTGAAACAGTTAGAAGCATTAGCTATAAAACGTGACTCATTTGGCCCAGGTAGTAGAATTATTATAACCACAAGAGACCAACATTTGCTAAAGATACTAAAAGCGAATAGGATATGTCATCTACCAGCAATGAATAAAGAAGAAGCTCTTGAGCTCCTTAGTTGGCATGCCTTCAGTAAGAATTATCCTAATAAAGAATATCTTCATGTCTCAAGAGAAGTTGTTGATTACTGTGGAGGTTTGCCACTAGCACTTGAAGTTTTAGGTTCTTATCTATCTGGGAAAAGCTCAAGTGAATGGAAAAGTGAGTTCGAGAAATTGAAATGTCATCCTCGTGGGGAGATTCTCAATAGACTTAAAATAAGCTTTGACGAGCTAGCTGATGATGACCTGAAGGCTATATTCCTTGATATATCATGTTTCTTTACTGGAATGAGCAAGGACCATGTCATGAAAATATTGGATGGCTGTGACTTATATCCAGAAATCGGAATCAGTGTCCTCCAAGAACGATGCCTTGTGACTACTAATGATGATTTCACACTGGTGATGCATGATTTGCTTCGCGACATGGGAAGAGAAATTGTGCGTTTAGAATCCCGTGACCCTGGAAAACGCAGTAGGTTATGGCATCACGACGATGTGCTAGATGTATTGATGAACAAATCTGGAACTGAAGCAATTCAAGGACTCACTTTAGATTTGCAAGAGTCTGACGATACTAGTTTCAGCACAGAAGCATTTAGAAACATGCATAGTCTGAGATTGCTCAAACTCAATTACGTCAAGCTCACTGGCAGTTACAACAATCTGTTCAATGATTTAAGATGGTTGTGTTGGCATGGATTCCCCCTGGAGGTCATACCAGAAGATTTTGATCACCCAAACATAGTTGTTATTGACCTGAGCTATAGCAAACTCACACGAGTTTGGGAGGATTCTGATCTGTTGCTTGAGAAGTTGAAGTTTCTTAATCTAAGTCATTCCCGTTACCTAAGACGATCACCTGACTTTTCAAAACTCCCAAATCTCGAGCAGTTGGTACTTCAGGACTGTAAGTCTTTGCTCAACATTGACCGGTCCATTGGTCAACTTGAGAGACTTGTTTCAGTAGATCTTAAAGACTGCTTGCTTAGGGATCTCCCAGAGAGTTTTTACAAATTGACATCTATTGAAACTCTTGATCTCACTGGCTGCTCAAATTTTGAAAACTTGGAGGACTTGGCTGGGAACGTGTTACTTGAGAAGTTAAAGTTTCTTTATCTTGGTTATTGCAATCGCCTGATCAGATTACCAGACTTTTCAAAAATCCCAAATCTTGAGAGATTGATACTTGAAGACTGTAACAGTTTGTCCGAGATTCACTCTGTAGTACGATTGAAAAAGCTTAAGTACTTGTCTCTTGCAAACTGCAATCTAGCAGATCATGCAATTCCTATAGATCTTGGGGCTCTATTTTCGTTAGAAGTTTTAGACGCAAGAGGCAATGGATTTAAATGCCTACCTATCCTCAGTAGACTTAACAAGCTTCAAACGTTACACTTGAATAATTGCACAAACCTTCAGAAAATTCCATATTTACCAACAAATTTGGAAATCCTGGAAGCGGATGAGTGCATTGCATTGGAGAAAATGCCAAATTTTTCGGAAATGTCAAGGATGAGAGTATTGCATCTGAATCACTCCCCCAAAATCACTGAGATTGGGGGCTTGCACAAGTCATTAAAGTCCATGACAAGGATTCATATGGAAGGGTGCACAAATCTCACTGCTGAGTTTAGGAAGAAGATACTACAGGGATGGACTTCTTGCGGATATGGTGGCATTTTCCTCAATGGAAATTATATTCCTGATTGGTTCAAGTATGTTGGGGATGATGAAGTCTCGATTGTAGTGCCTCAAGGGTTTGGTAGTAATTTTAATGGGTTGACTCTGTGCTGTGTATACTCTTCAAACAAACAACCTGAGGATTGTCCGCTTGTCATTTCCGTTCAAAATACAACCATGCGTACTGCTTTGCTCGCCCATATAACATATGCCTCGGTACCAACTTTCTGTGATTATGAAGATCATTATCTTTGGCAGGGGCAATTATCGAACGATGTGCTCCGTTGTCGAAAGGGGGATCAAGTCAACATTTTTGTAGGGCCTGCTCAGCCTGGAGATAATTCTGCAAGAGTGAAGAAAATAGCGGTTGATCTTGTCTGGGACAAATTTATGAAGGAAAATATGGATGATTCGCATCCTGGTTTATATGATTTAAACCCACATCAGGATTGTTTGTCGGGAGACAATGATGAGGCACGAACAAGCCGTGCCGCATCTGATGAGAACCTACCTTCGAAAATATTGAGGTCTGATAGTATTGGTGGTGGTGATGACGAGGTAGGAGCAAGCAATGATTTATCTGATGAGAACCAAACTCCCAAAAGAGGATTGTTTTATTTTTTTTCAAAACTGCTTCCGTCGTGTGGTAAAAAAACCAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

1137

Amino Acids

128.95

Weight (kDa)

5.68

Isoelectric Point (pI)

42.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 15 - 186 2.8e-34 TIR domain
TIR_2 PF13676 32 - 118 4.1e-07 TIR domain
NB-ARC PF00931 201 - 368 5.1e-19 NB-ARC domain
WHD_ROQ1 PF23282 439 - 506 1.9e-17 Disease resistance protein Roq1-like, winged-helix domain
LRR_14 PF23598 738 - 889 8.7e-12 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000055)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G36930 AT5G36930 AT5G36930 AT5G36930
fragaria_vesca FvH4_3g45361 FvH4_3g45380 FvH4_5g21061 FvH4_5g21062 FvH4_5g21063 FvH4_5g32050 FvH4_5g32970 FvH4_5g32990 FvH4_5g34190 FvH4_5g34210 FvH4_5g34210 FvH4_5g34210 FvH4_5g34210 FvH4_5g34210 FvH4_5g34210 FvH4_5g34211 FvH4_5g34240 FvH4_5g34250
malus_domestica MD00G1011700.v1.1 MD00G1026100.v1.1 MD00G1026500.v1.1 MD00G1027000.v1.1 MD00G1027100.v1.1 MD00G1027700.v1.1 MD00G1027800.v1.1 MD00G1028000.v1.1 MD00G1220000.v1.1 MD02G1024400.v1.1 MD02G1024800.v1.1 MD02G1026000.v1.1 MD02G1026100.v1.1 MD02G1031800.v1.1 MD02G1038500.v1.1 MD02G1038700.v1.1 MD02G1039200.v1.1 MD02G1040600.v1.1 MD02G1040900.v1.1 MD02G1041200.v1.1 MD02G1041300.v1.1 MD02G1041700.v1.1 MD02G1042000.v1.1 MD02G1043800.v1.1 MD02G1043900.v1.1 MD02G1044300.v1.1 MD02G1045200.v1.1 MD02G1045300.v1.1 MD02G1051200.v1.1 MD02G1052200.v1.1 MD02G1052800.v1.1 MD02G1053100.v1.1 MD02G1053600.v1.1 MD02G1055500.v1.1 MD02G1063300.v1.1 MD02G1063400.v1.1 MD02G1063800.v1.1 MD02G1306500.v1.1 MD02G1306600.v1.1 MD02G1306700.v1.1 MD02G1306900.v1.1 MD02G1307200.v1.1 MD04G1011700.v1.1 MD05G1315400.v1.1 MD05G1316000.v1.1 MD05G1316700.v1.1 MD05G1317000.v1.1 MD05G1317300.v1.1 MD05G1317600.v1.1 MD05G1317700.v1.1 MD07G1102800.v1.1 MD07G1238200.v1.1 MD08G1175900.v1.1 MD08G1203600.v1.1 MD09G1024200.v1.1 MD09G1039600.v1.1 MD09G1044800.v1.1 MD09G1045100.v1.1 MD10G1038700.v1.1 MD10G1039000.v1.1 MD10G1039200.v1.1 MD10G1040000.v1.1 MD10G1040200.v1.1 MD10G1040600.v1.1 MD10G1040800.v1.1 MD10G1297300.v1.1 MD12G1247500.v1.1 MD12G1247900.v1.1 MD12G1248300.v1.1 MD12G1248800.v1.1 MD15G1103700.v1.1 MD15G1103800.v1.1 MD15G1104000.v1.1 MD15G1179600.v1.1 MD15G1182500.v1.1 MD16G1068200.v1.1 MD16G1068300.v1.1 MD17G1024600.v1.1 MD17G1024700.v1.1 MD17G1025000.v1.1 MD17G1025900.v1.1
prunus_persica Prupe.1G539800_v2.0.a1 Prupe.1G550800_v2.0.a1 Prupe.1G550800_v2.0.a1 Prupe.1G550800_v2.0.a1 Prupe.1G550800_v2.0.a1 Prupe.1G550800_v2.0.a1 Prupe.1G550800_v2.0.a1 Prupe.1G557100_v2.0.a1 Prupe.1G557200_v2.0.a1 Prupe.1G557200_v2.0.a1 Prupe.1G557300_v2.0.a1
pyrus_communis pycom02g02080 pycom02g02090 pycom02g02110 pycom02g03220 pycom02g03260 pycom02g03370 pycom02g03390 pycom02g03410 pycom02g03420 pycom02g03450 pycom02g03500 pycom02g03730 pycom02g03770 pycom02g04400 pycom02g04410 pycom02g04430 pycom02g04450 pycom02g04460 pycom02g04490 pycom02g05200 pycom02g25710 pycom02g25750 pycom02g25790 pycom02g25810 pycom02g25830 pycom04g00920 pycom04g00930 pycom04g00940 pycom05g29300 pycom05g29310 pycom05g29380 pycom05g29410 pycom07g06310 pycom07g21420 pycom08g15070 pycom08g17540 pycom08g17560 pycom08g17570 pycom10g02670 pycom10g02680 pycom10g02700 pycom10g02770 pycom10g02790 pycom10g02800 pycom10g02810 pycom10g25020 pycom10g25030 pycom10g25050 pycom10g25080 pycom111g01990 pycom111g02000 pycom111g02030 pycom111g02040 pycom111g02050 pycom111g02060 pycom12g22710 pycom12g22720 pycom12g22730 pycom12g22740 pycom12g22750 pycom12g22770 pycom12g22780 pycom12g22790 pycom12g22800 pycom12g22810 pycom12g22830 pycom12g22840 pycom12g22860 pycom15g09440 pycom15g09480 pycom15g16120 pycom15g32380 pycom16g05970 pycom17g02100
rosa_chinensis RchiOBHm_Chr2g0144661 RchiOBHm_Chr4g0407491 RchiOBHm_Chr4g0407501 RchiOBHm_Chr4g0412381 RchiOBHm_Chr7g0208191 RchiOBHm_Chr7g0208201 RchiOBHm_Chr7g0208211 RchiOBHm_Chr7g0208221 RchiOBHm_Chr7g0228071 RchiOBHm_Chr7g0228121 RchiOBHm_Chr7g0230971 RchiOBHm_Chr7g0230981 RchiOBHm_Chr7g0233901 RchiOBHm_Chr7g0233951 RchiOBHm_Chr7g0233961 RchiOBHm_Chr7g0233991 RchiOBHm_Chr7g0234021 RchiOBHm_Chr7g0234151 RchiOBHm_Chr7g0234161 RchiOBHm_Chr7g0234241 RchiOBHm_Chr7g0234501 RchiOBHm_Chr7g0234641 RchiOBHm_Chr7g0234771 RchiOBHm_Chr7g0234781 RchiOBHm_Chr7g0234991 RchiOBHm_Chr7g0241291 RchiOBHm_Chr7g0241301
rosa_laevigata RLG00000001186 RLG00000001187 RLG00000001212 RLG00000001226 RLG00000001233 RLG00000001590 RLG00000003242 RLG00000003243 RLG00000003249 RLG00000008688 RLG00000008690
rosa_multiflora Rmu_co7966574.1_g000001 Rmu_co8120830.1_g000001 Rmu_co8142226.1_g000001 Rmu_co8293083.1_g000001 Rmu_co8351227.1_g000001 Rmu_co8380159.1_g000001 Rmu_co8456505.1_g000001 Rmu_sc0000676.1_g000025 Rmu_sc0000706.1_g000018 Rmu_sc0000706.1_g000040 Rmu_sc0000740.1_g000029 Rmu_sc0000740.1_g000032 Rmu_sc0000740.1_g000037 Rmu_sc0000740.1_g000040 Rmu_sc0000762.1_g000003 Rmu_sc0000762.1_g000019 Rmu_sc0000762.1_g000034 Rmu_sc0000762.1_g000069 Rmu_sc0000892.1_g000001 Rmu_sc0000945.1_g000040 Rmu_sc0001913.1_g000037 Rmu_sc0001913.1_g000041 Rmu_sc0001913.1_g000049 Rmu_sc0002775.1_g000017 Rmu_sc0002775.1_g000018 Rmu_sc0002775.1_g000020 Rmu_sc0002775.1_g000021 Rmu_sc0002775.1_g000022 Rmu_sc0002775.1_g000025 Rmu_sc0002775.1_g000026 Rmu_sc0002775.1_g000028 Rmu_sc0002775.1_g000030 Rmu_sc0004858.1_g000002 Rmu_sc0004864.1_g000022 Rmu_sc0005038.1_g000023 Rmu_sc0005840.1_g000014 Rmu_sc0006724.1_g000003 Rmu_sc0006824.1_g000005 Rmu_sc0007869.1_g000003 Rmu_sc0007869.1_g000005 Rmu_sc0007869.1_g000008 Rmu_sc0008646.1_g000007 Rmu_sc0009637.1_g000009 Rmu_sc0010500.1_g000002 Rmu_sc0010500.1_g000012 Rmu_sc0012403.1_g000025 Rmu_sc0013768.1_g000025 Rmu_sc0015625.1_g000001 Rmu_sc0019715.1_g000001 Rmu_sc0021133.1_g000002 Rmu_sc0021904.1_g000001 Rmu_sc0021904.1_g000003 Rmu_sc0021904.1_g000004 Rmu_sc0022054.1_g000001 Rmu_sc0026025.1_g000001 Rmu_sc0036828.1_g000002 Rmu_sc0041808.1_g000001 Rmu_ssc0000012.1_g000001
rosa_roxburghii Rroxscaffold_3G00226370 Rroxscaffold_3G00226860 Rroxscaffold_3G00226900 Rroxscaffold_3G00226930 Rroxscaffold_3G00226940 Rroxscaffold_3G00226970 Rroxscaffold_3G00226980 Rroxscaffold_3G00231530 Rroxscaffold_3G00237090 Rroxscaffold_3G00250760 Rroxscaffold_3G00250800
rosa_rugosa Rorug04G0082300 Rorug07G0101800 Rorug07G0102000 Rorug07G0102100 Rorug07G0221200 Rorug07G0245700 Rorug07G0245700 Rorug07G0245700 Rorug07G0263900 Rorug07G0264000 Rorug07G0264000 Rorug07G0264500 Rorug07G0282900 Rorug07G0282900 Rorug07G0283100 Rorug07G0283200.1 Rorug07G0283300 Rorug07G0283900 Rorug07G0284000 Rorug07G0284100 Rorug07G0284300 Rorug07G0284400 Rorug07G0284500 Rorug07G0285000 Rorug07G0285200 Rorug07G0285300 Rorug07G0285500 Rorug07G0286800 Rorug07G0286900 Rorug07G0287100 Rorug07G0287200
rosa_samantha Rh7AG236100 Rh7AG236200 Rh7AG236300 Rh7AG440000 Rh7AG440100 Rh7BG411900 Rh7BG412000 Rh7BG412600 Rh7CG251300 Rh7CG417700 Rh7CG460100 Rh7DG242100 Rh7DG242200 Rh7DG242300 Rh7DG429300
rosa_wichuraiana Rw0G005070 Rw0G010780 Rw0G012530 Rw7G020110 Rw7G020160 Rw7G020170 Rw7G033210 Rw7G033230 Rw7G033250 Rw7G034540 Rw7G035150 Rw7G036190 Rw7G036210 Rw7G036260 Rw7G036270 Rw7G036290 Rw7G036300 Rw7G036310 Rw7G036350 Rw7G036360 Rw7G036440 Rw7G036510 Rw7G036530 Rw7G036570 Rw7G036590 Rw7G036730

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 986
AasI GACNNNNNNGTC 1 cut(s) 19
AatII GACGTC 1 cut(s) 17
Acc65I GGTACC 1 cut(s) 2965
AccB1I GGYRCC 1 cut(s) 2965
AccI GTMKAC 3 cut(s) 153, 2437, 2865
AccII CGCG 2 cut(s) 122, 1488
AccIII TCCGGA 1 cut(s) 360
AciI CCGC 7 cut(s) 254, 562, 2528, 2733, 2897, 3113, 3240
AclI AACGTT 2 cut(s) 37, 2456
AclWI GGATC 3 cut(s) 649, 2067, 3054
AcsI RAATTY 8 cut(s) 469, 666, 730, 2122, 2490, 2509, 2560, 3134
AcuI CTGAAG 7 cut(s) 336, 480, 1078, 1334, 1617, 1952, 2468
AcyI GRCGYC 2 cut(s) 14, 420
AfaI GTAC 8 cut(s) 56, 376, 894, 1965, 2295, 2315, 2932, 2967
AfiI CCNNNNNNNGG 8 cut(s) 429, 1409, 1777, 2527, 2658, 3193, 3353, 3392
AflII CTTAAG 1 cut(s) 2309
AflIII ACRYGT 3 cut(s) 51, 120, 2154
AgeI ACCGGT 2 cut(s) 371, 1998
AhdI GACNNNNNGTC 1 cut(s) 1978
AhlI ACTAGT 1 cut(s) 1643
AjiI CACGTC 1 cut(s) 52
AjnI CCWGG 6 cut(s) 967, 1528, 1773, 2520, 3079, 3166
AjuI GAANNNNNNNTTGG 4 cut(s) 128, 160, 484, 516
AleI CACNNNNGTG 1 cut(s) 1466
Alw21I GWGCWC 3 cut(s) 1077, 2681, 3030
Alw26I GTCTC 7 cut(s) 441, 990, 1135, 2014, 2325, 2809, 3204
Alw44I GTGCAC 1 cut(s) 2677
AlwI GGATC 3 cut(s) 649, 2067, 3054
Ama87I CYCGRG 2 cut(s) 603, 1952
Aor13HI TCCGGA 1 cut(s) 360
AoxI GGCC 2 cut(s) 964, 3068
ApaLI GTGCAC 1 cut(s) 2677
ApeKI GCWGC 2 cut(s) 464, 2115
ApoI RAATTY 8 cut(s) 469, 666, 730, 2122, 2490, 2509, 2560, 3134
AsiGI ACCGGT 2 cut(s) 371, 1998
Asp700I GAANNNNTTC 3 cut(s) 598, 1122, 1602
Asp718I GGTACC 1 cut(s) 2965
AspS9I GGNCC 6 cut(s) 397, 855, 965, 1363, 2001, 3068
AsuHPI GGTGA 6 cut(s) 83, 793, 1480, 1793, 1917, 3308
AsuII TTCGAA 1 cut(s) 3263
AsuNHI GCTAGC 1 cut(s) 1297
AvaI CYCGRG 2 cut(s) 603, 1952
AvaII GGWCC 4 cut(s) 397, 855, 1363, 2001
AxyI CCTNAGG 1 cut(s) 2886
BaeGI GKGCMC 1 cut(s) 2681
BanI GGYRCC 1 cut(s) 2965
BanII GRGCYC 2 cut(s) 1077, 2374
BarI GAAGNNNNNNTAC 2 cut(s) 776, 808
BauI CACGAG 2 cut(s) 1254, 1845
BbsI GAAGAC 4 cut(s) 15, 229, 880, 2265
Bbv12I GWGCWC 3 cut(s) 1077, 2681, 3030
BbvI GCAGC 2 cut(s) 451, 2102
BccI CCATC 4 cut(s) 734, 1379, 1743, 2715
BceAI ACGGC 1 cut(s) 3219
BcgI CGANNNNNNTGC 4 cut(s) 2797, 2831, 3007, 3041
BciT130I CCWGG 6 cut(s) 969, 1530, 1775, 2522, 3081, 3168
BclI TGATCA 2 cut(s) 1798, 2205
BcoDI GTCTC 7 cut(s) 441, 990, 1135, 2014, 2325, 2809, 3204
BcuI ACTAGT 1 cut(s) 1643
BfaI CTAG 6 cut(s) 759, 1169, 1298, 1568, 1644, 2339
BfmI CTRYAG 4 cut(s) 1831, 2289, 2358, 2714
BfrI CTTAAG 1 cut(s) 2309
BglII AGATCT 2 cut(s) 2035, 2362
BisI GCNGC 3 cut(s) 465, 2116, 3240
BlpI GCTNAGC 2 cut(s) 574, 3075
BlsI GCNGC 3 cut(s) 466, 2117, 3241
BmcAI AGTACT 1 cut(s) 2315
Bme1390I CCNGG 6 cut(s) 969, 1530, 1775, 2522, 3081, 3168
Bme18I GGWCC 4 cut(s) 397, 855, 1363, 2001
BmeRI GACNNNNNGTC 1 cut(s) 1978
BmeT110I CYCGRG 2 cut(s) 603, 1952
BmgBI CACGTC 1 cut(s) 52
BmgT120I GGNCC 6 cut(s) 397, 855, 965, 1363, 2001, 3068
BmiI GGNNCC 2 cut(s) 856, 2967
BmrFI CCNGG 6 cut(s) 969, 1530, 1775, 2522, 3081, 3168
BmrI ACTGGG 1 cut(s) 320
BmsI GCATC 6 cut(s) 1424, 1461, 1561, 2602, 3172, 3251
BmtI GCTAGC 1 cut(s) 1301
BmuI ACTGGG 1 cut(s) 320
BpiI GAAGAC 4 cut(s) 15, 229, 880, 2265
BplI GAGNNNNNCTC 4 cut(s) 436, 468, 2270, 2302
BpmI CTGGAG 2 cut(s) 1796, 3102
Bpu10I CCTNAGC 2 cut(s) 1826, 2054
Bpu1102I GCTNAGC 2 cut(s) 574, 3075
Bpu14I TTCGAA 1 cut(s) 3263
BpuEI CTTGAG 9 cut(s) 79, 1091, 1117, 1195, 1895, 2036, 2183, 2261, 2805
BsaBI GATNNNNATC 5 cut(s) 198, 1797, 1863, 2994, 3162
BsaHI GRCGYC 2 cut(s) 14, 420
BsaI GGTCTC 1 cut(s) 990
BsaJI CCNNGG 6 cut(s) 211, 858, 967, 1528, 1773, 2961
BsaWI WCCGGW 3 cut(s) 360, 371, 1998
Bsc4I CCNNNNNNNGG 8 cut(s) 429, 1409, 1777, 2527, 2658, 3193, 3353, 3392
Bse118I RCCGGY 2 cut(s) 371, 1998
Bse1I ACTGG 7 cut(s) 315, 587, 783, 1354, 1470, 1720, 2116
Bse21I CCTNAGG 1 cut(s) 2886
Bse3DI GCAATG 4 cut(s) 1056, 2413, 2538, 3325
Bse8I GATNNNNATC 5 cut(s) 198, 1797, 1863, 2994, 3162
BseAI TCCGGA 1 cut(s) 360
BseBI CCWGG 6 cut(s) 969, 1530, 1775, 2522, 3081, 3168
BseDI CCNNGG 6 cut(s) 211, 858, 967, 1528, 1773, 2961
BseGI GGATG 9 cut(s) 10, 1249, 1390, 2536, 2586, 2726, 2800, 3160, 3163
BseJI GATNNNNATC 5 cut(s) 198, 1797, 1863, 2994, 3162
BseLI CCNNNNNNNGG 8 cut(s) 429, 1409, 1777, 2527, 2658, 3193, 3353, 3392
BseMI GCAATG 4 cut(s) 1056, 2413, 2538, 3325
BseMII CTCAG 8 cut(s) 176, 1673, 1817, 2446, 2610, 2685, 2877, 3089
BseNI ACTGG 7 cut(s) 315, 587, 783, 1354, 1470, 1720, 2116
BseSI GKGCMC 1 cut(s) 2681
BseXI GCAGC 2 cut(s) 451, 2102
BseYI CCCAGC 1 cut(s) 2147
BsgI GTGCAG 1 cut(s) 319
Bsh1236I CGCG 2 cut(s) 122, 1488
BshFI GGCC 2 cut(s) 966, 3070
BshNI GGYRCC 1 cut(s) 2965
BshTI ACCGGT 2 cut(s) 371, 1998
BsiHKAI GWGCWC 3 cut(s) 1077, 2681, 3030
BsiHKCI CYCGRG 2 cut(s) 603, 1952
BsiSI CCGG 3 cut(s) 361, 372, 1999
BslFI GGGAC 3 cut(s) 518, 868, 3143
BslI CCNNNNNNNGG 8 cut(s) 429, 1409, 1777, 2527, 2658, 3193, 3353, 3392
BsmAI GTCTC 7 cut(s) 441, 990, 1135, 2014, 2325, 2809, 3204
BsmBI CGTCTC 1 cut(s) 441
BsmFI GGGAC 3 cut(s) 518, 868, 3143
BsnI GGCC 2 cut(s) 966, 3070
Bso31I GGTCTC 1 cut(s) 990
BsoBI CYCGRG 2 cut(s) 603, 1952
Bsp119I TTCGAA 1 cut(s) 3263
Bsp1286I GDGCHC 4 cut(s) 1077, 2374, 2681, 3030
Bsp13I TCCGGA 1 cut(s) 360
Bsp1720I GCTNAGC 2 cut(s) 574, 3075
Bsp68I TCGCGA 1 cut(s) 1488
BspACI CCGC 7 cut(s) 254, 562, 2528, 2733, 2897, 3113, 3240
BspANI GGCC 2 cut(s) 966, 3070
BspCNI CTCAG 8 cut(s) 177, 1674, 1818, 2445, 2611, 2686, 2878, 3088
BspEI TCCGGA 1 cut(s) 360
BspFNI CGCG 2 cut(s) 122, 1488
BspHI TCATGA 1 cut(s) 1371
BspLI GGNNCC 2 cut(s) 856, 2967
BspOI GCTAGC 1 cut(s) 1301
BspPI GGATC 3 cut(s) 649, 2067, 3054
BspT104I TTCGAA 1 cut(s) 3263
BspT107I GGYRCC 1 cut(s) 2965
BspTI CTTAAG 1 cut(s) 2309
BspTNI GGTCTC 1 cut(s) 990
BsrDI GCAATG 4 cut(s) 1056, 2413, 2538, 3325
BsrFI RCCGGY 2 cut(s) 371, 1998
BsrI ACTGG 7 cut(s) 315, 587, 783, 1354, 1470, 1720, 2116
BssAI RCCGGY 2 cut(s) 371, 1998
BssECI CCNNGG 6 cut(s) 211, 858, 967, 1528, 1773, 2961
BssNAI GTATAC 1 cut(s) 2866
BssNI GRCGYC 2 cut(s) 14, 420
BssSI CACGAG 2 cut(s) 1254, 1845
BssT1I CCWWGG 1 cut(s) 211
Bst1107I GTATAC 1 cut(s) 2866
Bst2BI CACGAG 2 cut(s) 1254, 1845
Bst2UI CCWGG 6 cut(s) 969, 1530, 1775, 2522, 3081, 3168
Bst4CI ACNGT 6 cut(s) 700, 930, 1154, 1976, 2264, 2270
Bst6I CTCTTC 3 cut(s) 765, 1492, 2875
BstACI GRCGYC 2 cut(s) 14, 420
BstAFI CTTAAG 1 cut(s) 2309
BstAPI GCANNNNNTGC 3 cut(s) 570, 1480, 2348
BstBI TTCGAA 1 cut(s) 3263
BstC8I GCNNGC 8 cut(s) 132, 1089, 1299, 2051, 2633, 2943, 3072, 3316
BstF5I GGATG 9 cut(s) 10, 1249, 1390, 2536, 2586, 2726, 2800, 3160, 3163
BstFNI CGCG 2 cut(s) 122, 1488
BstMAI GTCTC 7 cut(s) 441, 990, 1135, 2014, 2325, 2809, 3204
BstMWI GCNNNNNNNGC 5 cut(s) 570, 1480, 2348, 2942, 3076
BstNI CCWGG 6 cut(s) 969, 1530, 1775, 2522, 3081, 3168
BstNSI RCATGY 2 cut(s) 1091, 1675
BstSCI CCNGG 6 cut(s) 967, 1528, 1773, 2520, 3079, 3166
BstSFI CTRYAG 4 cut(s) 1831, 2289, 2358, 2714
BstSLI GKGCMC 1 cut(s) 2681
BstUI CGCG 2 cut(s) 122, 1488
BstV1I GCAGC 2 cut(s) 451, 2102
BstV2I GAAGAC 4 cut(s) 15, 229, 880, 2265
BstX2I RGATCY 3 cut(s) 2035, 2059, 2362
BstYI RGATCY 3 cut(s) 2035, 2059, 2362
BstZ17I GTATAC 1 cut(s) 2866
Bsu36I CCTNAGG 1 cut(s) 2886
BsuRI GGCC 2 cut(s) 966, 3070
BtrI CACGTC 1 cut(s) 52
BtsCI GGATG 9 cut(s) 10, 1249, 1390, 2536, 2586, 2726, 2800, 3160, 3163
BtsI GCAGTG 1 cut(s) 2688
BtsIMutI CAGTG 7 cut(s) 308, 1423, 1463, 1713, 2109, 2616, 2688
BtuMI TCGCGA 1 cut(s) 1488
Cac8I GCNNGC 8 cut(s) 132, 1089, 1299, 2051, 2633, 2943, 3072, 3316
CciI TCATGA 1 cut(s) 1371
Cfr10I RCCGGY 2 cut(s) 371, 1998
Cfr13I GGNCC 6 cut(s) 397, 855, 965, 1363, 2001, 3068
CseI GACGC 3 cut(s) 428, 458, 2405
Csp6I GTAC 8 cut(s) 55, 375, 893, 1964, 2294, 2314, 2931, 2966
CspAI ACCGGT 2 cut(s) 371, 1998
CviQI GTAC 8 cut(s) 55, 375, 893, 1964, 2294, 2314, 2931, 2966
DraI TTTAAA 2 cut(s) 2416, 3183
DrdI GACNNNNNNGTC 1 cut(s) 19
DriI GACNNNNNGTC 1 cut(s) 1978
DseDI GACNNNNNNGTC 1 cut(s) 19
Eam1104I CTCTTC 3 cut(s) 765, 1492, 2875
Eam1105I GACNNNNNGTC 1 cut(s) 1978
EarI CTCTTC 3 cut(s) 765, 1492, 2875
Ecl136II GAGCTC 1 cut(s) 1075
Eco130I CCWWGG 1 cut(s) 211
Eco24I GRGCYC 2 cut(s) 1077, 2374
Eco31I GGTCTC 1 cut(s) 990
Eco47I GGWCC 4 cut(s) 397, 855, 1363, 2001
Eco53kI GAGCTC 1 cut(s) 1075
Eco57I CTGAAG 7 cut(s) 336, 480, 1078, 1334, 1617, 1952, 2468
Eco81I CCTNAGG 1 cut(s) 2886
Eco88I CYCGRG 2 cut(s) 603, 1952
EcoICRI GAGCTC 1 cut(s) 1075
EcoO109I RGGNCCY 1 cut(s) 3068
EcoRII CCWGG 6 cut(s) 967, 1528, 1773, 2520, 3079, 3166
EcoT14I CCWWGG 1 cut(s) 211
EcoT22I ATGCAT 2 cut(s) 1476, 1677
EcoT38I GRGCYC 2 cut(s) 1077, 2374
ErhI CCWWGG 1 cut(s) 211
Esp3I CGTCTC 1 cut(s) 441
FalI AAGNNNNNCTT 4 cut(s) 1053, 1085, 1107, 1139
FaqI GGGAC 3 cut(s) 518, 868, 3143
FauNDI CATATG 2 cut(s) 2667, 2956
FbaI TGATCA 2 cut(s) 1798, 2205
FblI GTMKAC 3 cut(s) 153, 2437, 2865
Fnu4HI GCNGC 3 cut(s) 465, 2116, 3240
FokI GGATG 9 cut(s) 17, 1236, 1397, 2543, 2593, 2733, 2807, 3150, 3167
FriOI GRGCYC 2 cut(s) 1077, 2374
Fsp4HI GCNGC 3 cut(s) 465, 2116, 3240
FspBI CTAG 6 cut(s) 759, 1169, 1298, 1568, 1644, 2339
GluI GCNGC 3 cut(s) 465, 2116, 3240
GsaI CCCAGC 1 cut(s) 2151
GsuI CTGGAG 2 cut(s) 1796, 3102
HaeIII GGCC 2 cut(s) 966, 3070
HapII CCGG 3 cut(s) 361, 372, 1999
HgaI GACGC 3 cut(s) 428, 458, 2405
Hin1I GRCGYC 2 cut(s) 14, 420
HincII GTYRAC 5 cut(s) 142, 154, 2012, 2850, 3055
HindII GTYRAC 5 cut(s) 142, 154, 2012, 2850, 3055
HindIII AAGCTT 3 cut(s) 1285, 2306, 2447
HpaII CCGG 3 cut(s) 361, 372, 1999
HphI GGTGA 6 cut(s) 83, 793, 1480, 1793, 1917, 3308
Hpy166II GTNNAC 9 cut(s) 55, 142, 154, 2012, 2438, 2679, 2850, 2866, 3055
Hpy8I GTNNAC 9 cut(s) 55, 142, 154, 2012, 2438, 2679, 2850, 2866, 3055
Hpy99I CGWCG 6 cut(s) 16, 53, 158, 700, 1562, 3391
HpyCH4III ACNGT 6 cut(s) 700, 930, 1154, 1976, 2264, 2270
HpyCH4IV ACGT 7 cut(s) 14, 37, 51, 952, 1704, 2154, 2456
HpyF10VI GCNNNNNNNGC 5 cut(s) 570, 1480, 2348, 2942, 3076
HpySE526I ACGT 7 cut(s) 14, 37, 51, 952, 1704, 2154, 2456
Hsp92I GRCGYC 2 cut(s) 14, 420
Kpn2I TCCGGA 1 cut(s) 360
KpnI GGTACC 1 cut(s) 2969
Ksp22I TGATCA 2 cut(s) 1798, 2205
LmnI GCTCC 3 cut(s) 1080, 3033, 3311
Lsp1109I GCAGC 2 cut(s) 451, 2102
LweI GCATC 6 cut(s) 1424, 1461, 1561, 2602, 3172, 3251
MaeI CTAG 6 cut(s) 759, 1169, 1298, 1568, 1644, 2339
MaeII ACGT 7 cut(s) 14, 37, 51, 952, 1704, 2154, 2456
MflI RGATCY 3 cut(s) 2035, 2059, 2362
MhlI GDGCHC 4 cut(s) 1077, 2374, 2681, 3030
MluI ACGCGT 1 cut(s) 120
MlyI GAGTC 5 cut(s) 561, 950, 1605, 1640, 2845
MmeI TCCRAC 4 cut(s) 20, 370, 571, 616
Mph1103I ATGCAT 2 cut(s) 1476, 1677
MroI TCCGGA 1 cut(s) 360
MroXI GAANNNNTTC 3 cut(s) 598, 1122, 1602
MslI CAYNNNNRTG 3 cut(s) 1254, 1466, 1560
MspCI CTTAAG 1 cut(s) 2309
MspI CCGG 3 cut(s) 361, 372, 1999
MspR9I CCNGG 6 cut(s) 969, 1530, 1775, 2522, 3081, 3168
MvaI CCWGG 6 cut(s) 969, 1530, 1775, 2522, 3081, 3168
MvnI CGCG 2 cut(s) 122, 1488
MwoI GCNNNNNNNGC 5 cut(s) 570, 1480, 2348, 2942, 3076
NdeI CATATG 2 cut(s) 2667, 2956
NheI GCTAGC 1 cut(s) 1297
NlaIV GGNNCC 2 cut(s) 856, 2967
NmuCI GTSAC 5 cut(s) 535, 953, 1391, 1441, 1523
NruI TCGCGA 1 cut(s) 1488
NsiI ATGCAT 2 cut(s) 1476, 1677
NspI RCATGY 2 cut(s) 1091, 1675
NspV TTCGAA 1 cut(s) 3263
OliI CACNNNNGTG 1 cut(s) 1466
PaeI GCATGC 1 cut(s) 1091
PaeR7I CTCGAG 1 cut(s) 1952
PagI TCATGA 1 cut(s) 1371
PcsI WCGNNNNNNNCGW 4 cut(s) 17, 101, 231, 3232
PdmI GAANNNNTTC 3 cut(s) 598, 1122, 1602
PflFI GACNNNGTC 1 cut(s) 1367
PfoI TCCNGGA 1 cut(s) 2520
PinAI ACCGGT 2 cut(s) 371, 1998
PkrI GCNGC 3 cut(s) 466, 2117, 3241
PleI GAGTC 5 cut(s) 560, 950, 1605, 1639, 2845
PpsI GAGTC 5 cut(s) 560, 950, 1605, 1639, 2845
PsiI TTATAA 1 cut(s) 986
Psp124BI GAGCTC 1 cut(s) 1077
Psp1406I AACGTT 2 cut(s) 37, 2456
Psp6I CCWGG 6 cut(s) 967, 1528, 1773, 2520, 3079, 3166
PspFI CCCAGC 1 cut(s) 2147
PspGI CCWGG 6 cut(s) 967, 1528, 1773, 2520, 3079, 3166
PspN4I GGNNCC 2 cut(s) 856, 2967
PspPI GGNCC 6 cut(s) 397, 855, 965, 1363, 2001, 3068
PsuI RGATCY 3 cut(s) 2035, 2059, 2362
PsyI GACNNNGTC 1 cut(s) 1367
RruI TCGCGA 1 cut(s) 1488
RsaI GTAC 8 cut(s) 56, 376, 894, 1965, 2295, 2315, 2932, 2967
RsaNI GTAC 8 cut(s) 55, 375, 893, 1964, 2294, 2314, 2931, 2966
RseI CAYNNNNRTG 3 cut(s) 1254, 1466, 1560
SacI GAGCTC 1 cut(s) 1077
SalI GTCGAC 1 cut(s) 152
SatI GCNGC 3 cut(s) 465, 2116, 3240
Sau96I GGNCC 6 cut(s) 397, 855, 965, 1363, 2001, 3068
ScaI AGTACT 1 cut(s) 2315
SchI GAGTC 5 cut(s) 561, 950, 1605, 1640, 2845
ScrFI CCNGG 6 cut(s) 969, 1530, 1775, 2522, 3081, 3168
SduI GDGCHC 4 cut(s) 1077, 2374, 2681, 3030
SfaNI GCATC 6 cut(s) 1424, 1461, 1561, 2602, 3172, 3251
SfcI CTRYAG 4 cut(s) 1831, 2289, 2358, 2714
Sfr274I CTCGAG 1 cut(s) 1952
SfuI TTCGAA 1 cut(s) 3263
SinI GGWCC 4 cut(s) 397, 855, 1363, 2001
SlaI CTCGAG 1 cut(s) 1952
SmiI ATTTAAAT 1 cut(s) 2416
SmiMI CAYNNNNRTG 3 cut(s) 1254, 1466, 1560
SpeI ACTAGT 1 cut(s) 1643
SphI GCATGC 1 cut(s) 1091
SsiI CCGC 7 cut(s) 254, 562, 2528, 2733, 2897, 3113, 3240
SspI AATATT 4 cut(s) 264, 333, 1380, 3270
SspMI CTAG 6 cut(s) 759, 1169, 1298, 1568, 1644, 2339
SstI GAGCTC 1 cut(s) 1077
StyD4I CCNGG 6 cut(s) 967, 1528, 1773, 2520, 3079, 3166
StyI CCWWGG 1 cut(s) 211
SwaI ATTTAAAT 1 cut(s) 2416
TaaI ACNGT 6 cut(s) 700, 930, 1154, 1976, 2264, 2270
TaiI ACGT 7 cut(s) 17, 40, 54, 955, 1707, 2157, 2459
TaqII GACCGA 1 cut(s) 872
TatI WGTACW 2 cut(s) 54, 2313
TauI GCSGC 1 cut(s) 3242
TscAI CASTG 7 cut(s) 315, 1423, 1470, 1720, 2116, 2623, 2695
TseFI GTSAC 5 cut(s) 535, 953, 1391, 1441, 1523
TseI GCWGC 2 cut(s) 464, 2115
Tsp45I GTSAC 5 cut(s) 535, 953, 1391, 1441, 1523
TspDTI ATGAA 9 cut(s) 864, 1067, 1115, 1388, 1595, 2654, 2814, 3000, 3155
TspGWI ACGGA 3 cut(s) 2899, 3020, 3375
TspRI CASTG 7 cut(s) 315, 1423, 1470, 1720, 2116, 2623, 2695
Tth111I GACNNNGTC 1 cut(s) 1367
Vha464I CTTAAG 1 cut(s) 2309
VneI GTGCAC 1 cut(s) 2677
VpaK11BI GGWCC 4 cut(s) 397, 855, 1363, 2001
XapI RAATTY 8 cut(s) 469, 666, 730, 2122, 2490, 2509, 2560, 3134
XceI RCATGY 2 cut(s) 1091, 1675
XhoI CTCGAG 1 cut(s) 1952
XmiI GTMKAC 3 cut(s) 153, 2437, 2865
XmnI GAANNNNTTC 3 cut(s) 598, 1122, 1602
XspI CTAG 6 cut(s) 759, 1169, 1298, 1568, 1644, 2339
ZraI GACGTC 1 cut(s) 15
ZrmI AGTACT 1 cut(s) 2315
Zsp2I ATGCAT 2 cut(s) 1476, 1677
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.