MD12G1247500.v1.1

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0000055
Physical Location & Seq
Reverse (-)
31790850 .. 31792730
1881 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1247500.v1.1.491

Sequence Viewer

Length: 1479 bp
ATGATTCCTCGTGGAGAAATTCAGGCACAACTAAAAATAAGCTACGACGGGCTAAATGATAATTACAAGAGGCGGATATTCCTCGATATAGCTTGTTTTTTTATCGGAATGGACAAGAACGATGTCGTGCAAATCTTGGATGGTTGTGGCTTTTATGCAACAACAGGAATCGAGGTCCTCTTTAACCGGTGCCTTGTGACTATTAATAGAGAAAACAAGATTATGATGCATGATTTGCTTCGGGATATGGGCAGAGATATCGTGCATGCAGAAAATCCCGATTTCCCTAGAGAACGGAGTAGATTGTGGCATCCTGAAGATGTAAATGATGTATTGATAGACAAATTTGGAACTGAAAAAATTGAAGGTCTGGCTTTGAATTTGCCAAGTCCTGAAGAGACTAGTTTCAGTACTAAGGCGTTTAGAAATATGAAGAGACTGAGATTGCTCCAACTAAACTACGTTCGGCTCACTGGGGGATACCAATGTCTTTCCAAAAAATTAAGATGGTTGTGCTGGCATGGATTCCCATTGGAGTTCATACCAATAGAACTGTGTCAACCAAACATAGTTGTTATCGACATGCAGTACAGCAGCCTCAGACAAGTTCTTTGTGAGTATTCTGGGTTGCTTGATAAGTTGAAGATTCTAAATCTCAGCCACTCCCACGATCTAACACAATCGCCAGACTTTTTGAAATTCCCAAATCTTGAGAAATTGATACTCAAAGACTGTAAGAGGTTGGCTAAAGTTCACAAGTCCATCGGAGATCTCAAGAGTCTTGTGTTGGTGAATTTGAAAGACTGTGAAACGCTTAAGGCTCTTCCGAGGAGTTTCTACAAGTTGAAATCTGTCAAAACTCTTGTTCTCGATGGTTGTTCAAGATTCCAAAGCTTGTCTGAGCACTTGGGAGAAATGGCATCATTGGTCACTCTTTATGCAAATGGGACGGCCATAAAAAAAGTACCACCTTCCATCGTACGACTAGAGAAGCTCGAGCTCTTATCTTTGAGTAAATTGAAGTGTTCTTTGCAGCTACCTTCCTTACAAGGTTTACGCTCTTTGACAAGCTTAATTTTGGCGGACAGCAATATAGAGGAAGTGCCTAATGATATTGGGAGTAGTCTACCTTGTTTAGTGGAGTTATGTGTAGATGACAATAATTTTGGGAGCCTTCCAAGCCTCAGTGACCTCTCCAAGCTTCTTATATTATCCTTGAACGGTTGCAGAAACCTTGTCGAGATTACAGATTTACCAAAAAGTTTGGGTTTCCTGCACATGGACGACTGCTCTGTATTAGAAAGAATGCCAGATTTTTCAGGCATGTCGACAACGGTGGTTCTCTTTTCCCCGAAACTCATTGAGTTTCCAGGCTTGGATAGTGCGTTAAACTCGGGACTCACACTTTTTATGATAACACACAACAATGTCATAGATTTCCTTCTTAAGGATAGCACGCTACAGGAGGTGGAACTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

493

Amino Acids

55.57

Weight (kDa)

6.24

Isoelectric Point (pI)

49.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WHD_ROQ1 PF23282 23 - 89 6.4e-19 Disease resistance protein Roq1-like, winged-helix domain
LRR_14 PF23598 295 - 358 6.6e-06 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000055)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G36930 AT5G36930 AT5G36930 AT5G36930
fragaria_vesca FvH4_3g45361 FvH4_3g45380 FvH4_5g21061 FvH4_5g21062 FvH4_5g21063 FvH4_5g32050 FvH4_5g32970 FvH4_5g32990 FvH4_5g34190 FvH4_5g34210 FvH4_5g34210 FvH4_5g34210 FvH4_5g34210 FvH4_5g34210 FvH4_5g34210 FvH4_5g34211 FvH4_5g34240 FvH4_5g34250
malus_domestica MD00G1011700.v1.1 MD00G1026100.v1.1 MD00G1026500.v1.1 MD00G1027000.v1.1 MD00G1027100.v1.1 MD00G1027700.v1.1 MD00G1027800.v1.1 MD00G1028000.v1.1 MD00G1220000.v1.1 MD02G1024400.v1.1 MD02G1024800.v1.1 MD02G1026000.v1.1 MD02G1026100.v1.1 MD02G1031800.v1.1 MD02G1038500.v1.1 MD02G1038700.v1.1 MD02G1039200.v1.1 MD02G1040600.v1.1 MD02G1040900.v1.1 MD02G1041200.v1.1 MD02G1041300.v1.1 MD02G1041700.v1.1 MD02G1042000.v1.1 MD02G1043800.v1.1 MD02G1043900.v1.1 MD02G1044300.v1.1 MD02G1045200.v1.1 MD02G1045300.v1.1 MD02G1051200.v1.1 MD02G1052200.v1.1 MD02G1052800.v1.1 MD02G1053100.v1.1 MD02G1053600.v1.1 MD02G1055500.v1.1 MD02G1063300.v1.1 MD02G1063400.v1.1 MD02G1063800.v1.1 MD02G1306500.v1.1 MD02G1306600.v1.1 MD02G1306700.v1.1 MD02G1306900.v1.1 MD02G1307200.v1.1 MD04G1011700.v1.1 MD05G1315400.v1.1 MD05G1316000.v1.1 MD05G1316700.v1.1 MD05G1317000.v1.1 MD05G1317300.v1.1 MD05G1317600.v1.1 MD05G1317700.v1.1 MD07G1102800.v1.1 MD07G1238200.v1.1 MD08G1175900.v1.1 MD08G1203600.v1.1 MD09G1024200.v1.1 MD09G1039600.v1.1 MD09G1044800.v1.1 MD09G1045100.v1.1 MD10G1038700.v1.1 MD10G1039000.v1.1 MD10G1039200.v1.1 MD10G1040000.v1.1 MD10G1040200.v1.1 MD10G1040600.v1.1 MD10G1040800.v1.1 MD10G1297300.v1.1 MD12G1247500.v1.1 MD12G1247900.v1.1 MD12G1248300.v1.1 MD12G1248800.v1.1 MD15G1103700.v1.1 MD15G1103800.v1.1 MD15G1104000.v1.1 MD15G1179600.v1.1 MD15G1182500.v1.1 MD16G1068200.v1.1 MD16G1068300.v1.1 MD17G1024600.v1.1 MD17G1024700.v1.1 MD17G1025000.v1.1 MD17G1025900.v1.1
prunus_persica Prupe.1G539800_v2.0.a1 Prupe.1G550800_v2.0.a1 Prupe.1G550800_v2.0.a1 Prupe.1G550800_v2.0.a1 Prupe.1G550800_v2.0.a1 Prupe.1G550800_v2.0.a1 Prupe.1G550800_v2.0.a1 Prupe.1G557100_v2.0.a1 Prupe.1G557200_v2.0.a1 Prupe.1G557200_v2.0.a1 Prupe.1G557300_v2.0.a1
pyrus_communis pycom02g02080 pycom02g02090 pycom02g02110 pycom02g03220 pycom02g03260 pycom02g03370 pycom02g03390 pycom02g03410 pycom02g03420 pycom02g03450 pycom02g03500 pycom02g03730 pycom02g03770 pycom02g04400 pycom02g04410 pycom02g04430 pycom02g04450 pycom02g04460 pycom02g04490 pycom02g05200 pycom02g25710 pycom02g25750 pycom02g25790 pycom02g25810 pycom02g25830 pycom04g00920 pycom04g00930 pycom04g00940 pycom05g29300 pycom05g29310 pycom05g29380 pycom05g29410 pycom07g06310 pycom07g21420 pycom08g15070 pycom08g17540 pycom08g17560 pycom08g17570 pycom10g02670 pycom10g02680 pycom10g02700 pycom10g02770 pycom10g02790 pycom10g02800 pycom10g02810 pycom10g25020 pycom10g25030 pycom10g25050 pycom10g25080 pycom111g01990 pycom111g02000 pycom111g02030 pycom111g02040 pycom111g02050 pycom111g02060 pycom12g22710 pycom12g22720 pycom12g22730 pycom12g22740 pycom12g22750 pycom12g22770 pycom12g22780 pycom12g22790 pycom12g22800 pycom12g22810 pycom12g22830 pycom12g22840 pycom12g22860 pycom15g09440 pycom15g09480 pycom15g16120 pycom15g32380 pycom16g05970 pycom17g02100
rosa_chinensis RchiOBHm_Chr2g0144661 RchiOBHm_Chr4g0407491 RchiOBHm_Chr4g0407501 RchiOBHm_Chr4g0412381 RchiOBHm_Chr7g0208191 RchiOBHm_Chr7g0208201 RchiOBHm_Chr7g0208211 RchiOBHm_Chr7g0208221 RchiOBHm_Chr7g0228071 RchiOBHm_Chr7g0228121 RchiOBHm_Chr7g0230971 RchiOBHm_Chr7g0230981 RchiOBHm_Chr7g0233901 RchiOBHm_Chr7g0233951 RchiOBHm_Chr7g0233961 RchiOBHm_Chr7g0233991 RchiOBHm_Chr7g0234021 RchiOBHm_Chr7g0234151 RchiOBHm_Chr7g0234161 RchiOBHm_Chr7g0234241 RchiOBHm_Chr7g0234501 RchiOBHm_Chr7g0234641 RchiOBHm_Chr7g0234771 RchiOBHm_Chr7g0234781 RchiOBHm_Chr7g0234991 RchiOBHm_Chr7g0241291 RchiOBHm_Chr7g0241301
rosa_laevigata RLG00000001186 RLG00000001187 RLG00000001212 RLG00000001226 RLG00000001233 RLG00000001590 RLG00000003242 RLG00000003243 RLG00000003249 RLG00000008688 RLG00000008690
rosa_multiflora Rmu_co7966574.1_g000001 Rmu_co8120830.1_g000001 Rmu_co8142226.1_g000001 Rmu_co8293083.1_g000001 Rmu_co8351227.1_g000001 Rmu_co8380159.1_g000001 Rmu_co8456505.1_g000001 Rmu_sc0000676.1_g000025 Rmu_sc0000706.1_g000018 Rmu_sc0000706.1_g000040 Rmu_sc0000740.1_g000029 Rmu_sc0000740.1_g000032 Rmu_sc0000740.1_g000037 Rmu_sc0000740.1_g000040 Rmu_sc0000762.1_g000003 Rmu_sc0000762.1_g000019 Rmu_sc0000762.1_g000034 Rmu_sc0000762.1_g000069 Rmu_sc0000892.1_g000001 Rmu_sc0000945.1_g000040 Rmu_sc0001913.1_g000037 Rmu_sc0001913.1_g000041 Rmu_sc0001913.1_g000049 Rmu_sc0002775.1_g000017 Rmu_sc0002775.1_g000018 Rmu_sc0002775.1_g000020 Rmu_sc0002775.1_g000021 Rmu_sc0002775.1_g000022 Rmu_sc0002775.1_g000025 Rmu_sc0002775.1_g000026 Rmu_sc0002775.1_g000028 Rmu_sc0002775.1_g000030 Rmu_sc0004858.1_g000002 Rmu_sc0004864.1_g000022 Rmu_sc0005038.1_g000023 Rmu_sc0005840.1_g000014 Rmu_sc0006724.1_g000003 Rmu_sc0006824.1_g000005 Rmu_sc0007869.1_g000003 Rmu_sc0007869.1_g000005 Rmu_sc0007869.1_g000008 Rmu_sc0008646.1_g000007 Rmu_sc0009637.1_g000009 Rmu_sc0010500.1_g000002 Rmu_sc0010500.1_g000012 Rmu_sc0012403.1_g000025 Rmu_sc0013768.1_g000025 Rmu_sc0015625.1_g000001 Rmu_sc0019715.1_g000001 Rmu_sc0021133.1_g000002 Rmu_sc0021904.1_g000001 Rmu_sc0021904.1_g000003 Rmu_sc0021904.1_g000004 Rmu_sc0022054.1_g000001 Rmu_sc0026025.1_g000001 Rmu_sc0036828.1_g000002 Rmu_sc0041808.1_g000001 Rmu_ssc0000012.1_g000001
rosa_roxburghii Rroxscaffold_3G00226370 Rroxscaffold_3G00226860 Rroxscaffold_3G00226900 Rroxscaffold_3G00226930 Rroxscaffold_3G00226940 Rroxscaffold_3G00226970 Rroxscaffold_3G00226980 Rroxscaffold_3G00231530 Rroxscaffold_3G00237090 Rroxscaffold_3G00250760 Rroxscaffold_3G00250800
rosa_rugosa Rorug04G0082300 Rorug07G0101800 Rorug07G0102000 Rorug07G0102100 Rorug07G0221200 Rorug07G0245700 Rorug07G0245700 Rorug07G0245700 Rorug07G0263900 Rorug07G0264000 Rorug07G0264000 Rorug07G0264500 Rorug07G0282900 Rorug07G0282900 Rorug07G0283100 Rorug07G0283200.1 Rorug07G0283300 Rorug07G0283900 Rorug07G0284000 Rorug07G0284100 Rorug07G0284300 Rorug07G0284400 Rorug07G0284500 Rorug07G0285000 Rorug07G0285200 Rorug07G0285300 Rorug07G0285500 Rorug07G0286800 Rorug07G0286900 Rorug07G0287100 Rorug07G0287200
rosa_samantha Rh7AG236100 Rh7AG236200 Rh7AG236300 Rh7AG440000 Rh7AG440100 Rh7BG411900 Rh7BG412000 Rh7BG412600 Rh7CG251300 Rh7CG417700 Rh7CG460100 Rh7DG242100 Rh7DG242200 Rh7DG242300 Rh7DG429300
rosa_wichuraiana Rw0G005070 Rw0G010780 Rw0G012530 Rw7G020110 Rw7G020160 Rw7G020170 Rw7G033210 Rw7G033230 Rw7G033250 Rw7G034540 Rw7G035150 Rw7G036190 Rw7G036210 Rw7G036260 Rw7G036270 Rw7G036290 Rw7G036300 Rw7G036310 Rw7G036350 Rw7G036360 Rw7G036440 Rw7G036510 Rw7G036530 Rw7G036570 Rw7G036590 Rw7G036730

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 189
AccI GTMKAC 2 cut(s) 1126, 1328
AciI CCGC 2 cut(s) 73, 1082
AcoI YGGCCR 1 cut(s) 951
AcsI RAATTY 5 cut(s) 18, 344, 379, 698, 793
AcuI CTGAAG 2 cut(s) 336, 414
AfaI GTAC 4 cut(s) 412, 590, 966, 981
AfiI CCNNNNNNNGG 2 cut(s) 1279, 1447
AflII CTTAAG 2 cut(s) 815, 1445
AgeI ACCGGT 1 cut(s) 186
AgsI TTSAA 9 cut(s) 365, 379, 643, 697, 799, 847, 882, 1021, 1219
AhlI ACTAGT 1 cut(s) 401
AjnI CCWGG 1 cut(s) 1369
AluBI AGCT 8 cut(s) 42, 92, 894, 994, 1000, 1036, 1071, 1201
AluI AGCT 8 cut(s) 42, 92, 894, 994, 1000, 1036, 1071, 1201
Alw21I GWGCWC 2 cut(s) 906, 1002
Alw26I GTCTC 2 cut(s) 392, 430
Ama87I CYCGRG 2 cut(s) 995, 1393
AoxI GGCC 1 cut(s) 951
ApeKI GCWGC 2 cut(s) 594, 1033
ApoI RAATTY 5 cut(s) 18, 344, 379, 698, 793
ArsI GACNNNNNNTTYG 4 cut(s) 594, 626, 1148, 1180
AseI ATTAAT 1 cut(s) 204
AsiGI ACCGGT 1 cut(s) 186
AspS9I GGNCC 1 cut(s) 175
AsuHPI GGTGA 1 cut(s) 802
AvaI CYCGRG 2 cut(s) 995, 1393
AvaII GGWCC 1 cut(s) 175
BaeI ACNNNNGTAYC 2 cut(s) 472, 505
BanI GGYRCC 1 cut(s) 189
BanII GRGCYC 1 cut(s) 1002
BauI CACGAG 1 cut(s) 9
Bbv12I GWGCWC 2 cut(s) 906, 1002
BbvI GCAGC 2 cut(s) 606, 1045
BccI CCATC 5 cut(s) 134, 501, 770, 866, 983
BceAI ACGGC 1 cut(s) 966
BcgI CGANNNNNNTGC 2 cut(s) 241, 275
BciT130I CCWGG 1 cut(s) 1371
BciVI GTATCC 1 cut(s) 473
BcoDI GTCTC 2 cut(s) 392, 430
BcuI ACTAGT 1 cut(s) 401
BfaI CTAG 3 cut(s) 288, 402, 986
BfmI CTRYAG 1 cut(s) 1460
BfrI CTTAAG 2 cut(s) 815, 1445
BfuI GTATCC 1 cut(s) 473
BglII AGATCT 1 cut(s) 769
BisI GCNGC 2 cut(s) 595, 1034
BlsI GCNGC 2 cut(s) 596, 1035
BmcAI AGTACT 1 cut(s) 412
Bme1390I CCNGG 1 cut(s) 1371
Bme18I GGWCC 1 cut(s) 175
BmeT110I CYCGRG 2 cut(s) 995, 1393
BmgT120I GGNCC 1 cut(s) 175
BmiI GGNNCC 2 cut(s) 191, 1172
BmrFI CCNGG 1 cut(s) 1371
BmrI ACTGGG 1 cut(s) 483
BmsI GCATC 3 cut(s) 216, 319, 929
BmuI ACTGGG 1 cut(s) 483
BpuEI CTTGAG 2 cut(s) 731, 758
BsaJI CCNNGG 1 cut(s) 827
BsaWI WCCGGW 1 cut(s) 186
Bsc4I CCNNNNNNNGG 2 cut(s) 1279, 1447
Bse118I RCCGGY 1 cut(s) 186
Bse1I ACTGG 1 cut(s) 478
BseBI CCWGG 1 cut(s) 1371
BseDI CCNNGG 1 cut(s) 827
BseGI GGATG 2 cut(s) 145, 310
BseLI CCNNNNNNNGG 2 cut(s) 1279, 1447
BseMII CTCAG 5 cut(s) 431, 613, 670, 891, 1198
BseNI ACTGG 1 cut(s) 478
BseRI GAGGAG 1 cut(s) 844
BseXI GCAGC 2 cut(s) 606, 1045
BsgI GTGCAG 1 cut(s) 1259
BshFI GGCC 1 cut(s) 953
BshNI GGYRCC 1 cut(s) 189
BshTI ACCGGT 1 cut(s) 186
BsiHKAI GWGCWC 2 cut(s) 906, 1002
BsiHKCI CYCGRG 2 cut(s) 995, 1393
BsiSI CCGG 1 cut(s) 187
BsiWI CGTACG 1 cut(s) 979
BslFI GGGAC 2 cut(s) 961, 1410
BslI CCNNNNNNNGG 2 cut(s) 1279, 1447
BsmAI GTCTC 2 cut(s) 392, 430
BsmFI GGGAC 2 cut(s) 961, 1410
BsmI GAATGC 1 cut(s) 1311
BsnI GGCC 1 cut(s) 953
BsoBI CYCGRG 2 cut(s) 995, 1393
Bsp1286I GDGCHC 2 cut(s) 906, 1002
Bsp143I GATC 2 cut(s) 670, 769
BspACI CCGC 2 cut(s) 73, 1082
BspANI GGCC 1 cut(s) 953
BspCNI CTCAG 5 cut(s) 432, 612, 669, 892, 1197
BspLI GGNNCC 2 cut(s) 191, 1172
BspQI GCTCTTC 1 cut(s) 828
BspT107I GGYRCC 1 cut(s) 189
BspTI CTTAAG 2 cut(s) 815, 1445
BsrFI RCCGGY 1 cut(s) 186
BsrI ACTGG 1 cut(s) 478
BssAI RCCGGY 1 cut(s) 186
BssECI CCNNGG 1 cut(s) 827
BssMI GATC 2 cut(s) 670, 769
BssSI CACGAG 1 cut(s) 9
Bst2BI CACGAG 1 cut(s) 9
Bst2UI CCWGG 1 cut(s) 1371
Bst4CI ACNGT 5 cut(s) 555, 734, 806, 1223, 1336
Bst6I CTCTTC 3 cut(s) 390, 428, 828
BstAFI CTTAAG 2 cut(s) 815, 1445
BstAPI GCANNNNNTGC 1 cut(s) 235
BstC8I GCNNGC 3 cut(s) 267, 518, 1457
BstDEI CTNAG 6 cut(s) 414, 440, 599, 656, 900, 1184
BstENI CCTNNNNNAGG 1 cut(s) 1445
BstF5I GGATG 2 cut(s) 145, 310
BstKTI GATC 2 cut(s) 673, 772
BstMAI GTCTC 2 cut(s) 392, 430
BstMBI GATC 2 cut(s) 670, 769
BstMWI GCNNNNNNNGC 2 cut(s) 235, 1179
BstNI CCWGG 1 cut(s) 1371
BstNSI RCATGY 3 cut(s) 269, 586, 1327
BstSCI CCNGG 1 cut(s) 1369
BstSFI CTRYAG 1 cut(s) 1460
BstV1I GCAGC 2 cut(s) 606, 1045
BstX2I RGATCY 1 cut(s) 769
BstYI RGATCY 1 cut(s) 769
BsuI GTATCC 1 cut(s) 473
BsuRI GGCC 1 cut(s) 953
BtsCI GGATG 2 cut(s) 145, 310
BtsIMutI CAGTG 2 cut(s) 471, 1192
Cac8I GCNNGC 3 cut(s) 267, 518, 1457
Cfr10I RCCGGY 1 cut(s) 186
Cfr13I GGNCC 1 cut(s) 175
Csp6I GTAC 4 cut(s) 411, 589, 965, 980
CspAI ACCGGT 1 cut(s) 186
CviAII CATG 6 cut(s) 230, 266, 521, 583, 1279, 1324
CviQI GTAC 4 cut(s) 411, 589, 965, 980
DdeI CTNAG 6 cut(s) 414, 440, 599, 656, 900, 1184
DpnI GATC 2 cut(s) 672, 771
DpnII GATC 2 cut(s) 670, 769
EaeI YGGCCR 1 cut(s) 951
Eam1104I CTCTTC 3 cut(s) 390, 428, 828
EarI CTCTTC 3 cut(s) 390, 428, 828
EciI GGCGGA 2 cut(s) 88, 1097
Ecl136II GAGCTC 1 cut(s) 1000
Eco24I GRGCYC 1 cut(s) 1002
Eco32I GATATC 1 cut(s) 259
Eco47I GGWCC 1 cut(s) 175
Eco53kI GAGCTC 1 cut(s) 1000
Eco57I CTGAAG 2 cut(s) 336, 414
Eco88I CYCGRG 2 cut(s) 995, 1393
EcoICRI GAGCTC 1 cut(s) 1000
EcoNI CCTNNNNNAGG 1 cut(s) 1445
EcoO109I RGGNCCY 1 cut(s) 175
EcoRII CCWGG 1 cut(s) 1369
EcoRV GATATC 1 cut(s) 259
EcoT22I ATGCAT 1 cut(s) 231
EcoT38I GRGCYC 1 cut(s) 1002
FaeI CATG 6 cut(s) 233, 269, 524, 586, 1282, 1327
FaqI GGGAC 2 cut(s) 961, 1410
FatI CATG 6 cut(s) 229, 265, 520, 582, 1278, 1323
FblI GTMKAC 2 cut(s) 1126, 1328
Fnu4HI GCNGC 2 cut(s) 595, 1034
FokI GGATG 2 cut(s) 152, 297
FriOI GRGCYC 1 cut(s) 1002
Fsp4HI GCNGC 2 cut(s) 595, 1034
FspBI CTAG 3 cut(s) 288, 402, 986
GluI GCNGC 2 cut(s) 595, 1034
HaeIII GGCC 1 cut(s) 953
HapII CCGG 1 cut(s) 187
Hin1II CATG 6 cut(s) 233, 269, 524, 586, 1282, 1327
HincII GTYRAC 2 cut(s) 560, 1329
HindII GTYRAC 2 cut(s) 560, 1329
HindIII AAGCTT 3 cut(s) 892, 1069, 1199
HinfI GANTC 7 cut(s) 4, 168, 525, 646, 778, 885, 1398
HpaII CCGG 1 cut(s) 187
HphI GGTGA 1 cut(s) 802
Hpy166II GTNNAC 5 cut(s) 560, 754, 1055, 1127, 1329
Hpy188I TCNGA 5 cut(s) 107, 602, 767, 828, 901
Hpy8I GTNNAC 5 cut(s) 560, 754, 1055, 1127, 1329
Hpy99I CGWCG 1 cut(s) 50
HpyAV CCTTC 5 cut(s) 359, 981, 1050, 1184, 1451
HpyCH4III ACNGT 5 cut(s) 555, 734, 806, 1223, 1336
HpyCH4IV ACGT 1 cut(s) 462
HpyF10VI GCNNNNNNNGC 2 cut(s) 235, 1179
HpyF3I CTNAG 6 cut(s) 414, 440, 599, 656, 900, 1184
HpySE526I ACGT 1 cut(s) 462
Hsp92II CATG 6 cut(s) 233, 269, 524, 586, 1282, 1327
Kzo9I GATC 2 cut(s) 670, 769
LguI GCTCTTC 1 cut(s) 828
LmnI GCTCC 2 cut(s) 453, 1170
Lsp1109I GCAGC 2 cut(s) 606, 1045
LweI GCATC 3 cut(s) 216, 319, 929
MaeI CTAG 3 cut(s) 288, 402, 986
MaeII ACGT 1 cut(s) 462
MaeIII GTNAC 3 cut(s) 196, 928, 1187
MalI GATC 2 cut(s) 672, 771
MboI GATC 2 cut(s) 670, 769
MboII GAAGA 5 cut(s) 329, 407, 445, 655, 815
MflI RGATCY 1 cut(s) 769
MhlI GDGCHC 2 cut(s) 906, 1002
MlyI GAGTC 2 cut(s) 787, 1392
MmeI TCCRAC 1 cut(s) 475
Mph1103I ATGCAT 1 cut(s) 231
MseI TTAA 7 cut(s) 183, 204, 503, 816, 1073, 1388, 1446
MslI CAYNNNNRTG 1 cut(s) 1425
MspCI CTTAAG 2 cut(s) 815, 1445
MspI CCGG 1 cut(s) 187
MspR9I CCNGG 1 cut(s) 1371
Mva1269I GAATGC 1 cut(s) 1311
MvaI CCWGG 1 cut(s) 1371
MwoI GCNNNNNNNGC 2 cut(s) 235, 1179
NdeII GATC 2 cut(s) 670, 769
NlaIII CATG 6 cut(s) 233, 269, 524, 586, 1282, 1327
NlaIV GGNNCC 2 cut(s) 191, 1172
NmuCI GTSAC 3 cut(s) 196, 928, 1187
NsiI ATGCAT 1 cut(s) 231
NspI RCATGY 3 cut(s) 269, 586, 1327
PaeI GCATGC 1 cut(s) 269
PaeR7I CTCGAG 1 cut(s) 995
PciSI GCTCTTC 1 cut(s) 828
PctI GAATGC 1 cut(s) 1311
PfeI GAWTC 5 cut(s) 4, 168, 525, 646, 885
Pfl23II CGTACG 1 cut(s) 979
PinAI ACCGGT 1 cut(s) 186
PkrI GCNGC 2 cut(s) 596, 1035
PleI GAGTC 2 cut(s) 786, 1392
PpsI GAGTC 2 cut(s) 786, 1392
PpuMI RGGWCCY 1 cut(s) 175
PshBI ATTAAT 1 cut(s) 204
Psp124BI GAGCTC 1 cut(s) 1002
Psp5II RGGWCCY 1 cut(s) 175
Psp6I CCWGG 1 cut(s) 1369
PspGI CCWGG 1 cut(s) 1369
PspLI CGTACG 1 cut(s) 979
PspN4I GGNNCC 2 cut(s) 191, 1172
PspPI GGNCC 1 cut(s) 175
PspPPI RGGWCCY 1 cut(s) 175
PspXI VCTCGAGB 1 cut(s) 995
PsuI RGATCY 1 cut(s) 769
RsaI GTAC 4 cut(s) 412, 590, 966, 981
RsaNI GTAC 4 cut(s) 411, 589, 965, 980
RseI CAYNNNNRTG 1 cut(s) 1425
SacI GAGCTC 1 cut(s) 1002
SalI GTCGAC 1 cut(s) 1327
SapI GCTCTTC 1 cut(s) 828
SaqAI TTAA 7 cut(s) 183, 204, 503, 816, 1073, 1388, 1446
SatI GCNGC 2 cut(s) 595, 1034
Sau3AI GATC 2 cut(s) 670, 769
Sau96I GGNCC 1 cut(s) 175
ScaI AGTACT 1 cut(s) 412
SchI GAGTC 2 cut(s) 787, 1392
ScrFI CCNGG 1 cut(s) 1371
SduI GDGCHC 2 cut(s) 906, 1002
SfaNI GCATC 3 cut(s) 216, 319, 929
SfcI CTRYAG 1 cut(s) 1460
Sfr274I CTCGAG 1 cut(s) 995
SinI GGWCC 1 cut(s) 175
SlaI CTCGAG 1 cut(s) 995
SmiMI CAYNNNNRTG 1 cut(s) 1425
SmlI CTYRAG 5 cut(s) 710, 773, 815, 995, 1445
SmoI CTYRAG 5 cut(s) 710, 773, 815, 995, 1445
SpeI ACTAGT 1 cut(s) 401
SphI GCATGC 1 cut(s) 269
SsiI CCGC 2 cut(s) 73, 1082
SspMI CTAG 3 cut(s) 288, 402, 986
SstI GAGCTC 1 cut(s) 1002
StyD4I CCNGG 1 cut(s) 1369
TaaI ACNGT 5 cut(s) 555, 734, 806, 1223, 1336
TaiI ACGT 1 cut(s) 465
TaqI TCGA 7 cut(s) 84, 171, 579, 870, 996, 1239, 1328
TatI WGTACW 2 cut(s) 410, 588
TfiI GAWTC 5 cut(s) 4, 168, 525, 646, 885
Tru1I TTAA 7 cut(s) 183, 204, 503, 816, 1073, 1388, 1446
Tru9I TTAA 7 cut(s) 183, 204, 503, 816, 1073, 1388, 1446
TscAI CASTG 2 cut(s) 478, 1192
TseFI GTSAC 3 cut(s) 196, 928, 1187
TseI GCWGC 2 cut(s) 594, 1033
Tsp45I GTSAC 3 cut(s) 196, 928, 1187
TspDTI ATGAA 2 cut(s) 446, 529
TspGWI ACGGA 1 cut(s) 310
TspRI CASTG 2 cut(s) 478, 1192
Vha464I CTTAAG 2 cut(s) 815, 1445
VpaK11BI GGWCC 1 cut(s) 175
VspI ATTAAT 1 cut(s) 204
XagI CCTNNNNNAGG 1 cut(s) 1445
XapI RAATTY 5 cut(s) 18, 344, 379, 698, 793
XceI RCATGY 3 cut(s) 269, 586, 1327
XhoI CTCGAG 1 cut(s) 995
XmiI GTMKAC 2 cut(s) 1126, 1328
XspI CTAG 3 cut(s) 288, 402, 986
ZrmI AGTACT 1 cut(s) 412
Zsp2I ATGCAT 1 cut(s) 231
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.