MD02G1044300.v1.1

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0000055
Physical Location & Seq
Reverse (-)
3616412 .. 3620336
3925 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1044300.v1.1.491

Sequence Viewer

Length: 3135 bp
ATGACAACCCACGAAGCCTCCTCTTCATCCTCCTCCAAGCCAAAACTTTGGAATTACGAAGTGTTTTTGAGCTTCAGCGGTGCAGACACACGCAATGGCTTCACGGGCCACCTCCACGCGGCTTTAAAAGACAGGGGATATCAGGCTTATTTCGATGAGGACGATATAGAAAGAGGGGAAGAAATCAAAGACCAACTGTTCCGGGCGATCGAAGAGTCGAGGATCTGTATCGTTGTCTTCTCAAAGAGGTATGCGGATTCGAGTTGGTGTCTTGAGGAGCTGGTGAAGATCATGGAGTGCAGATCCAAACTGGAGCGACATGTTTTGCCAATATTCTATCATGTTGATCCTTCACATGTCAGGAAGCAGGACGGAGATTTAGCCGAAGCATTTCTGAAGCACGAAGAGGGCATCAGTGAAGAAAAGGATGACAAGAAACGTGAAGCTAAACAAGAAAGAGTAAAACAGTGGAGAGAGGCTCTTACAGAAGCTGCAAATTTGTCTGGCCACCATCTTCAAATCACTGACAATAGGCGCGAAGCAAAGCTTATTAGAGGAATTGTTGACAAGATTATTACTAAATGGCTTCCAAGCACAAACAAATTATATGTGGCCAAGCACCAAGTTGGAATCAATTCTCGCATTCAAAATATTATCGCTTTTCTTTCAAGTGGTGGACCAAATAATGTTATCATGGTTGGAATTTGGGGGATGGGTGAATTGGGTAAAACAACAGTTGCCAAAGCCATTTATAACCAAATTCATTGTAAGTTTGAATTCAAAAGTTTCCTTGCCGACGTTAGCGACACTACAAGTAAACATGGTCTAGTTTATTTGCAAGAAACACTAGTTTCTAACATCTTGAAAAAGAAGTATAAAATAAGCAGTGTTGATGAAGGTATCAGTCTGATAGAACAACACTTTCAACGTAGAAGGGTACTTGTCATCGTGGACAATATAGATGAAGTGGAACAACTCAATGCAATAGCTGGAAATCGTGATTGGTATGGTCCAGGAAGTAGAATTATCATAACAACAAGAGATGAACATTTACTAAAGCAAGTGGAAGTGGACAAGATATATCCGCTTCAGAAAATGAATGAAGAAGAAGCTCTGGAGCTCTTTAGTTGGCATGCATTTAGAAATAGTTGGCCTAACGAAGGATATCTTGAACTCGCAAAAAAGGTTGTTTCTTACTGCGGAGGCTTGCCACTAGCACTTGAAGTTTTAGGCTCTTTTTTGATTAAAAGAACCATATCAGAGTGGGGAAGTCAATTGGAGAAATTGGAAAAAATTCCTGATGGAAAAATAATAAAACCACTAAGAATAAGTTTCGAAGGGCTAGATGATACACAAAAGGCTATATTCCTTGACATATCTTGTTTCTTTATTGGATGGAATAAGGACTATGTCATAAAAGTATTAGATGGATGTGAATTTTCTGCAACAATAGGAATCAGTGTTCTTCGTGAACGATGCCTTGTAACTGTTGAACGCAACGAGTTGAATATGCATGATTTGCTTCGAGAAATGGCCAGAGTAATCATTTCTGAAAAATCCCCTGATCACCCTGGAAAATGGAGTAGGTTGTGGAACCCTCAAGTCGTCACCAATGTATTAACAAATAAAACTGGAACTGAAGGAGTCGAAGGACTTGCTCTACCAAGGACCAGTTGTTTCACTACAATAGCATTTACCAATATGAAGAAACTGAGATTGCTTCAGCTCAACTTCGTAGAGCTCAATGGAGAATACAAACATCTTCCCAACGAGTTAATATGGTTGTGCTGGCATGGATTCCCTTTAAAGTCCATACCAGATGACTTTATTAATCAACCAAGACTAGTTGTTTTAGACATGCAGTTTAGCCAACTGCTACAAGTTTGGGAGGGTTCCAAGTCGTTGCTGAAGTTGAAAATCATTAATCTCAGTTATTCCCGTTTGCTAATTAAATCACCGGAGTTTTCACAACTCCCAAATCTTGAAGAGCTGATATTGGAATGCTGTTTTAGTTTGTCCGAGATTCACCCCTCCATTAGTCATCTTGAAAGACTGTCTTTGGTGAACCTTACGAGATGTTACAAGCTTCGTTCTCTTCCAAGGGATTTCTATAAGTCGAAATCTGTTGAGACTCTTCTTCTTAATGGATGTTTCATAGAACTACATGAGGATTTAGGGGAGATGGTATCGTTGAGAGTACTTGAAGCAGAGAGTACAGCCATAAGGCACGTACCACCTTCCATGGTGAGACTGAAAAATTTCACTCGTTTATCCCTAGAATGTGTGCAATTGTTGGGGTTGCCTATTCCTGAGGATCTTGGGAGTCTAATTTCCTTACAAGATTTGAATCTTCGAAGGAATGATTTTCGTAGCCTACCCAGCCTCACTGGTCTTTCAAAGCTCGAAACTCTACGGCTAAGTGGATGCAGAAAGCTTCAAACGATCCCTGATTTACCAACAAATTTGAAATTTCTGTATGCATTTGATTGCCCTGCATTGAGAATTGTGCCAAAGTTATCAGAAAGGGCAAATGGGAGACAGCTGAATGTAAGTGATTCAACCAAACTCACTAAAGTTGTAGGTTCCGGTAAGTCATTAAACACCATGACATGGATTGATATGAAAAGGTGCACCAATCTCACAGCTGAGTTTAGGAAGAACATCCTACAGGGATGGACTTCTTGTGGATTTGGTGGCATTTTCCTCCGTGGAAATTATGTTCCTGATTGGTTTGAGTTTGTCAACGAGGGCAATAAAGTCAGTTTTGTTATTCCCCCAAGTGATGAGCGTAATCTTGAAGGGCTGACTCTATTCTGCAATTTTGAAAGGTTGACTCTGTTTTGCTTTTATTGCTCAGGCAATAGATTCAATCGTCCTCTTCCTCTTGCCAGTATCACTGTAATAAATAATACCAAATGTACAGAGTTGGGGGATTGCATAGTCAGAGAAGATTGGGAGGGGCTAATAGATAACAAACCAGCCCATTATATTTGGCAGGGACAATTATCGAACCATAAGCTCAATTTGCAAGGCAAGGATATAGTTGAAGTTGATATAATTTTTGCGGAATCTGATTTTCCTCAGACAGTGAAGAGAACAGGGGTTAATCCAGTATGGGACAAACCTATGGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

1045

Amino Acids

119.35

Weight (kDa)

7.29

Isoelectric Point (pI)

38.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 18 - 201 2.8e-49 TIR domain
TIR_2 PF13676 21 - 118 4.2e-19 TIR domain
NB-ARC PF00931 214 - 382 2.7e-17 NB-ARC domain
WHD_ROQ1 PF23282 451 - 520 2.8e-14 Disease resistance protein Roq1-like, winged-helix domain
LRR_14 PF23598 715 - 805 1.2e-06 Leucine-rich repeat region
LRR_4 PF12799 778 - 819 8.6e-06 Leucine Rich repeats (2 copies)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000055)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G36930 AT5G36930 AT5G36930 AT5G36930
fragaria_vesca FvH4_3g45361 FvH4_3g45380 FvH4_5g21061 FvH4_5g21062 FvH4_5g21063 FvH4_5g32050 FvH4_5g32970 FvH4_5g32990 FvH4_5g34190 FvH4_5g34210 FvH4_5g34210 FvH4_5g34210 FvH4_5g34210 FvH4_5g34210 FvH4_5g34210 FvH4_5g34211 FvH4_5g34240 FvH4_5g34250
malus_domestica MD00G1011700.v1.1 MD00G1026100.v1.1 MD00G1026500.v1.1 MD00G1027000.v1.1 MD00G1027100.v1.1 MD00G1027700.v1.1 MD00G1027800.v1.1 MD00G1028000.v1.1 MD00G1220000.v1.1 MD02G1024400.v1.1 MD02G1024800.v1.1 MD02G1026000.v1.1 MD02G1026100.v1.1 MD02G1031800.v1.1 MD02G1038500.v1.1 MD02G1038700.v1.1 MD02G1039200.v1.1 MD02G1040600.v1.1 MD02G1040900.v1.1 MD02G1041200.v1.1 MD02G1041300.v1.1 MD02G1041700.v1.1 MD02G1042000.v1.1 MD02G1043800.v1.1 MD02G1043900.v1.1 MD02G1044300.v1.1 MD02G1045200.v1.1 MD02G1045300.v1.1 MD02G1051200.v1.1 MD02G1052200.v1.1 MD02G1052800.v1.1 MD02G1053100.v1.1 MD02G1053600.v1.1 MD02G1055500.v1.1 MD02G1063300.v1.1 MD02G1063400.v1.1 MD02G1063800.v1.1 MD02G1306500.v1.1 MD02G1306600.v1.1 MD02G1306700.v1.1 MD02G1306900.v1.1 MD02G1307200.v1.1 MD04G1011700.v1.1 MD05G1315400.v1.1 MD05G1316000.v1.1 MD05G1316700.v1.1 MD05G1317000.v1.1 MD05G1317300.v1.1 MD05G1317600.v1.1 MD05G1317700.v1.1 MD07G1102800.v1.1 MD07G1238200.v1.1 MD08G1175900.v1.1 MD08G1203600.v1.1 MD09G1024200.v1.1 MD09G1039600.v1.1 MD09G1044800.v1.1 MD09G1045100.v1.1 MD10G1038700.v1.1 MD10G1039000.v1.1 MD10G1039200.v1.1 MD10G1040000.v1.1 MD10G1040200.v1.1 MD10G1040600.v1.1 MD10G1040800.v1.1 MD10G1297300.v1.1 MD12G1247500.v1.1 MD12G1247900.v1.1 MD12G1248300.v1.1 MD12G1248800.v1.1 MD15G1103700.v1.1 MD15G1103800.v1.1 MD15G1104000.v1.1 MD15G1179600.v1.1 MD15G1182500.v1.1 MD16G1068200.v1.1 MD16G1068300.v1.1 MD17G1024600.v1.1 MD17G1024700.v1.1 MD17G1025000.v1.1 MD17G1025900.v1.1
prunus_persica Prupe.1G539800_v2.0.a1 Prupe.1G550800_v2.0.a1 Prupe.1G550800_v2.0.a1 Prupe.1G550800_v2.0.a1 Prupe.1G550800_v2.0.a1 Prupe.1G550800_v2.0.a1 Prupe.1G550800_v2.0.a1 Prupe.1G557100_v2.0.a1 Prupe.1G557200_v2.0.a1 Prupe.1G557200_v2.0.a1 Prupe.1G557300_v2.0.a1
pyrus_communis pycom02g02080 pycom02g02090 pycom02g02110 pycom02g03220 pycom02g03260 pycom02g03370 pycom02g03390 pycom02g03410 pycom02g03420 pycom02g03450 pycom02g03500 pycom02g03730 pycom02g03770 pycom02g04400 pycom02g04410 pycom02g04430 pycom02g04450 pycom02g04460 pycom02g04490 pycom02g05200 pycom02g25710 pycom02g25750 pycom02g25790 pycom02g25810 pycom02g25830 pycom04g00920 pycom04g00930 pycom04g00940 pycom05g29300 pycom05g29310 pycom05g29380 pycom05g29410 pycom07g06310 pycom07g21420 pycom08g15070 pycom08g17540 pycom08g17560 pycom08g17570 pycom10g02670 pycom10g02680 pycom10g02700 pycom10g02770 pycom10g02790 pycom10g02800 pycom10g02810 pycom10g25020 pycom10g25030 pycom10g25050 pycom10g25080 pycom111g01990 pycom111g02000 pycom111g02030 pycom111g02040 pycom111g02050 pycom111g02060 pycom12g22710 pycom12g22720 pycom12g22730 pycom12g22740 pycom12g22750 pycom12g22770 pycom12g22780 pycom12g22790 pycom12g22800 pycom12g22810 pycom12g22830 pycom12g22840 pycom12g22860 pycom15g09440 pycom15g09480 pycom15g16120 pycom15g32380 pycom16g05970 pycom17g02100
rosa_chinensis RchiOBHm_Chr2g0144661 RchiOBHm_Chr4g0407491 RchiOBHm_Chr4g0407501 RchiOBHm_Chr4g0412381 RchiOBHm_Chr7g0208191 RchiOBHm_Chr7g0208201 RchiOBHm_Chr7g0208211 RchiOBHm_Chr7g0208221 RchiOBHm_Chr7g0228071 RchiOBHm_Chr7g0228121 RchiOBHm_Chr7g0230971 RchiOBHm_Chr7g0230981 RchiOBHm_Chr7g0233901 RchiOBHm_Chr7g0233951 RchiOBHm_Chr7g0233961 RchiOBHm_Chr7g0233991 RchiOBHm_Chr7g0234021 RchiOBHm_Chr7g0234151 RchiOBHm_Chr7g0234161 RchiOBHm_Chr7g0234241 RchiOBHm_Chr7g0234501 RchiOBHm_Chr7g0234641 RchiOBHm_Chr7g0234771 RchiOBHm_Chr7g0234781 RchiOBHm_Chr7g0234991 RchiOBHm_Chr7g0241291 RchiOBHm_Chr7g0241301
rosa_laevigata RLG00000001186 RLG00000001187 RLG00000001212 RLG00000001226 RLG00000001233 RLG00000001590 RLG00000003242 RLG00000003243 RLG00000003249 RLG00000008688 RLG00000008690
rosa_multiflora Rmu_co7966574.1_g000001 Rmu_co8120830.1_g000001 Rmu_co8142226.1_g000001 Rmu_co8293083.1_g000001 Rmu_co8351227.1_g000001 Rmu_co8380159.1_g000001 Rmu_co8456505.1_g000001 Rmu_sc0000676.1_g000025 Rmu_sc0000706.1_g000018 Rmu_sc0000706.1_g000040 Rmu_sc0000740.1_g000029 Rmu_sc0000740.1_g000032 Rmu_sc0000740.1_g000037 Rmu_sc0000740.1_g000040 Rmu_sc0000762.1_g000003 Rmu_sc0000762.1_g000019 Rmu_sc0000762.1_g000034 Rmu_sc0000762.1_g000069 Rmu_sc0000892.1_g000001 Rmu_sc0000945.1_g000040 Rmu_sc0001913.1_g000037 Rmu_sc0001913.1_g000041 Rmu_sc0001913.1_g000049 Rmu_sc0002775.1_g000017 Rmu_sc0002775.1_g000018 Rmu_sc0002775.1_g000020 Rmu_sc0002775.1_g000021 Rmu_sc0002775.1_g000022 Rmu_sc0002775.1_g000025 Rmu_sc0002775.1_g000026 Rmu_sc0002775.1_g000028 Rmu_sc0002775.1_g000030 Rmu_sc0004858.1_g000002 Rmu_sc0004864.1_g000022 Rmu_sc0005038.1_g000023 Rmu_sc0005840.1_g000014 Rmu_sc0006724.1_g000003 Rmu_sc0006824.1_g000005 Rmu_sc0007869.1_g000003 Rmu_sc0007869.1_g000005 Rmu_sc0007869.1_g000008 Rmu_sc0008646.1_g000007 Rmu_sc0009637.1_g000009 Rmu_sc0010500.1_g000002 Rmu_sc0010500.1_g000012 Rmu_sc0012403.1_g000025 Rmu_sc0013768.1_g000025 Rmu_sc0015625.1_g000001 Rmu_sc0019715.1_g000001 Rmu_sc0021133.1_g000002 Rmu_sc0021904.1_g000001 Rmu_sc0021904.1_g000003 Rmu_sc0021904.1_g000004 Rmu_sc0022054.1_g000001 Rmu_sc0026025.1_g000001 Rmu_sc0036828.1_g000002 Rmu_sc0041808.1_g000001 Rmu_ssc0000012.1_g000001
rosa_roxburghii Rroxscaffold_3G00226370 Rroxscaffold_3G00226860 Rroxscaffold_3G00226900 Rroxscaffold_3G00226930 Rroxscaffold_3G00226940 Rroxscaffold_3G00226970 Rroxscaffold_3G00226980 Rroxscaffold_3G00231530 Rroxscaffold_3G00237090 Rroxscaffold_3G00250760 Rroxscaffold_3G00250800
rosa_rugosa Rorug04G0082300 Rorug07G0101800 Rorug07G0102000 Rorug07G0102100 Rorug07G0221200 Rorug07G0245700 Rorug07G0245700 Rorug07G0245700 Rorug07G0263900 Rorug07G0264000 Rorug07G0264000 Rorug07G0264500 Rorug07G0282900 Rorug07G0282900 Rorug07G0283100 Rorug07G0283200.1 Rorug07G0283300 Rorug07G0283900 Rorug07G0284000 Rorug07G0284100 Rorug07G0284300 Rorug07G0284400 Rorug07G0284500 Rorug07G0285000 Rorug07G0285200 Rorug07G0285300 Rorug07G0285500 Rorug07G0286800 Rorug07G0286900 Rorug07G0287100 Rorug07G0287200
rosa_samantha Rh7AG236100 Rh7AG236200 Rh7AG236300 Rh7AG440000 Rh7AG440100 Rh7BG411900 Rh7BG412000 Rh7BG412600 Rh7CG251300 Rh7CG417700 Rh7CG460100 Rh7DG242100 Rh7DG242200 Rh7DG242300 Rh7DG429300
rosa_wichuraiana Rw0G005070 Rw0G010780 Rw0G012530 Rw7G020110 Rw7G020160 Rw7G020170 Rw7G033210 Rw7G033230 Rw7G033250 Rw7G034540 Rw7G035150 Rw7G036190 Rw7G036210 Rw7G036260 Rw7G036270 Rw7G036290 Rw7G036300 Rw7G036310 Rw7G036350 Rw7G036360 Rw7G036440 Rw7G036510 Rw7G036530 Rw7G036570 Rw7G036590 Rw7G036730

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 753
AccB7I CCANNNNNTGG 1 cut(s) 2610
AccII CGCG 2 cut(s) 119, 537
AciI CCGC 6 cut(s) 78, 119, 254, 1085, 1200, 3065
AclWI GGATC 5 cut(s) 230, 297, 341, 2322, 2437
AcoI YGGCCR 3 cut(s) 505, 612, 1533
AcsI RAATTY 9 cut(s) 496, 702, 759, 776, 1293, 1436, 2257, 2461, 2468
AcuI CTGAAG 6 cut(s) 58, 416, 1073, 1659, 1706, 1928
AfaI GTAC 5 cut(s) 939, 2199, 2215, 2232, 2920
AfiI CCNNNNNNNGG 3 cut(s) 118, 1160, 2610
AflIII ACRYGT 2 cut(s) 319, 355
AhlI ACTAGT 2 cut(s) 847, 1843
AjnI CCWGG 2 cut(s) 1012, 1570
AjuI GAANNNNNNNTTGG 2 cut(s) 985, 1017
AloI GAACNNNNNNTCC 4 cut(s) 1446, 1478, 2703, 2735
Alw21I GWGCWC 3 cut(s) 1122, 1743, 2633
Alw26I GTCTC 3 cut(s) 2123, 2242, 2530
Alw44I GTGCAC 1 cut(s) 2629
AlwI GGATC 5 cut(s) 230, 297, 341, 2322, 2437
AlwNI CAGNNNCTG 1 cut(s) 491
AoxI GGCC 5 cut(s) 106, 505, 612, 1151, 1533
ApaLI GTGCAC 1 cut(s) 2629
ApeKI GCWGC 1 cut(s) 491
ApoI RAATTY 9 cut(s) 496, 702, 759, 776, 1293, 1436, 2257, 2461, 2468
AseI ATTAAT 2 cut(s) 1830, 1923
Asp700I GAANNNNTTC 4 cut(s) 390, 634, 1293, 2258
AspLEI GCGC 1 cut(s) 537
AspS9I GGNCC 4 cut(s) 106, 677, 1010, 1668
AsuC2I CCSGG 1 cut(s) 203
AsuHPI GGTGA 8 cut(s) 295, 728, 1559, 1600, 1947, 2018, 2074, 2257
AsuII TTCGAA 2 cut(s) 1335, 2353
AvaII GGWCC 3 cut(s) 677, 1010, 1668
AxyI CCTNAGG 1 cut(s) 2310
BaeGI GKGCMC 1 cut(s) 2633
BalI TGGCCA 3 cut(s) 507, 614, 1535
BanII GRGCYC 2 cut(s) 1122, 1743
BbsI GAAGAC 1 cut(s) 229
Bbv12I GWGCWC 3 cut(s) 1122, 1743, 2633
BbvI GCAGC 1 cut(s) 478
BccI CCATC 7 cut(s) 519, 706, 1295, 1389, 1421, 2176, 2667
BceAI ACGGC 1 cut(s) 2429
BcgI CGANNNNNNTGC 2 cut(s) 1637, 1671
BciT130I CCWGG 2 cut(s) 1014, 1572
BclI TGATCA 1 cut(s) 1564
BcnI CCSGG 1 cut(s) 203
BcoDI GTCTC 3 cut(s) 2123, 2242, 2530
BcuI ACTAGT 2 cut(s) 847, 1843
BfaI CTAG 6 cut(s) 827, 848, 1214, 1343, 1844, 2276
BfmI CTRYAG 1 cut(s) 2666
BisI GCNGC 2 cut(s) 120, 492
BlsI GCNGC 2 cut(s) 121, 493
BmcAI AGTACT 1 cut(s) 2199
Bme1390I CCNGG 3 cut(s) 203, 1014, 1572
Bme18I GGWCC 3 cut(s) 677, 1010, 1668
BmgT120I GGNCC 4 cut(s) 106, 677, 1010, 1668
BmiI GGNNCC 3 cut(s) 1595, 1894, 2584
BmrFI CCNGG 3 cut(s) 203, 1014, 1572
BmsI GCATC 3 cut(s) 420, 1466, 2414
BpiI GAAGAC 1 cut(s) 229
BplI GAGNNNNNCTC 2 cut(s) 463, 495
BpmI CTGGAG 2 cut(s) 332, 1136
Bpu10I CCTNAGC 1 cut(s) 2854
Bpu14I TTCGAA 2 cut(s) 1335, 2353
BpuEI CTTGAG 2 cut(s) 293, 1584
BpuMI CCSGG 1 cut(s) 203
BsaAI YACGTR 1 cut(s) 2230
BsaBI GATNNNNATC 2 cut(s) 227, 2346
BsaJI CCNNGG 5 cut(s) 1570, 1664, 2099, 2241, 2707
BsaWI WCCGGW 2 cut(s) 1957, 2585
BsaXI ACNNNNNCTCC 1 cut(s) 32
Bsc4I CCNNNNNNNGG 3 cut(s) 118, 1160, 2610
Bse1I ACTGG 6 cut(s) 315, 1636, 1671, 2392, 2889, 3110
Bse21I CCTNAGG 1 cut(s) 2310
Bse3DI GCAATG 1 cut(s) 100
Bse8I GATNNNNATC 2 cut(s) 227, 2346
BseBI CCWGG 2 cut(s) 1014, 1572
BseDI CCNNGG 5 cut(s) 1570, 1664, 2099, 2241, 2707
BseGI GGATG 9 cut(s) 26, 433, 717, 1400, 1436, 2153, 2429, 2661, 2678
BseJI GATNNNNATC 2 cut(s) 227, 2346
BseLI CCNNNNNNNGG 3 cut(s) 118, 1160, 2610
BseMI GCAATG 1 cut(s) 100
BseMII CTCAG 6 cut(s) 1703, 1942, 2301, 2637, 2868, 3095
BseNI ACTGG 6 cut(s) 315, 1636, 1671, 2392, 2889, 3110
BseRI GAGGAG 3 cut(s) 10, 22, 290
BseSI GKGCMC 1 cut(s) 2633
BseXI GCAGC 1 cut(s) 478
BseYI CCCAGC 1 cut(s) 2378
BsgI GTGCAG 2 cut(s) 102, 319
Bsh1236I CGCG 2 cut(s) 119, 537
Bsh1285I CGRYCG 1 cut(s) 210
BshFI GGCC 5 cut(s) 108, 507, 614, 1153, 1535
BsiEI CGRYCG 1 cut(s) 210
BsiHKAI GWGCWC 3 cut(s) 1122, 1743, 2633
BsiSI CCGG 3 cut(s) 202, 1958, 2586
BslFI GGGAC 2 cut(s) 3012, 3131
BslI CCNNNNNNNGG 3 cut(s) 118, 1160, 2610
BsmAI GTCTC 3 cut(s) 2123, 2242, 2530
BsmFI GGGAC 2 cut(s) 3012, 3131
BsmI GAATGC 2 cut(s) 642, 2006
BsnI GGCC 5 cut(s) 108, 507, 614, 1153, 1535
Bsp119I TTCGAA 2 cut(s) 1335, 2353
Bsp1286I GDGCHC 3 cut(s) 1122, 1743, 2633
Bsp1407I TGTACA 1 cut(s) 2918
Bsp143I GATC 8 cut(s) 207, 222, 288, 302, 346, 1564, 2314, 2442
Bsp19I CCATGG 1 cut(s) 2241
BspACI CCGC 6 cut(s) 78, 119, 254, 1085, 1200, 3065
BspANI GGCC 5 cut(s) 108, 507, 614, 1153, 1535
BspCNI CTCAG 6 cut(s) 1704, 1941, 2302, 2638, 2867, 3094
BspFNI CGCG 2 cut(s) 119, 537
BspLI GGNNCC 3 cut(s) 1595, 1894, 2584
BspPI GGATC 5 cut(s) 230, 297, 341, 2322, 2437
BspQI GCTCTTC 1 cut(s) 1980
BspT104I TTCGAA 2 cut(s) 1335, 2353
BsrDI GCAATG 1 cut(s) 100
BsrGI TGTACA 1 cut(s) 2918
BsrI ACTGG 6 cut(s) 315, 1636, 1671, 2392, 2889, 3110
BssECI CCNNGG 5 cut(s) 1570, 1664, 2099, 2241, 2707
BssMI GATC 8 cut(s) 207, 222, 288, 302, 346, 1564, 2314, 2442
BssT1I CCWWGG 3 cut(s) 1664, 2099, 2241
Bst2UI CCWGG 2 cut(s) 1014, 1572
Bst4CI ACNGT 7 cut(s) 198, 468, 736, 1489, 2055, 2899, 3088
Bst6I CTCTTC 8 cut(s) 28, 207, 399, 1980, 2100, 2139, 2883, 3086
BstAPI GCANNNNNTGC 1 cut(s) 1519
BstAUI TGTACA 1 cut(s) 2918
BstBAI YACGTR 1 cut(s) 2230
BstBI TTCGAA 2 cut(s) 1335, 2353
BstC8I GCNNGC 3 cut(s) 1134, 1208, 1790
BstDEI CTNAG 8 cut(s) 1322, 1712, 1928, 2310, 2417, 2646, 2854, 3081
BstDSI CCRYGG 2 cut(s) 2241, 2707
BstENI CCTNNNNNAGG 1 cut(s) 1158
BstF5I GGATG 9 cut(s) 26, 433, 717, 1400, 1436, 2153, 2429, 2661, 2678
BstFNI CGCG 2 cut(s) 119, 537
BstHHI GCGC 1 cut(s) 537
BstKTI GATC 8 cut(s) 210, 225, 291, 305, 349, 1567, 2317, 2445
BstMAI GTCTC 3 cut(s) 2123, 2242, 2530
BstMBI GATC 8 cut(s) 207, 222, 288, 302, 346, 1564, 2314, 2442
BstMCI CGRYCG 1 cut(s) 210
BstMWI GCNNNNNNNGC 5 cut(s) 105, 1519, 2379, 2850, 3025
BstNI CCWGG 2 cut(s) 1014, 1572
BstNSI RCATGY 4 cut(s) 323, 359, 1136, 1861
BstSCI CCNGG 3 cut(s) 201, 1012, 1570
BstSFI CTRYAG 1 cut(s) 2666
BstSLI GKGCMC 1 cut(s) 2633
BstUI CGCG 2 cut(s) 119, 537
BstV1I GCAGC 1 cut(s) 478
BstV2I GAAGAC 1 cut(s) 229
BstX2I RGATCY 3 cut(s) 222, 302, 2314
BstXI CCANNNNNNTGG 1 cut(s) 48
BstYI RGATCY 3 cut(s) 222, 302, 2314
Bsu36I CCTNAGG 1 cut(s) 2310
BsuRI GGCC 5 cut(s) 108, 507, 614, 1153, 1535
BtgI CCRYGG 2 cut(s) 2241, 2707
BtsCI GGATG 9 cut(s) 26, 433, 717, 1400, 1436, 2153, 2429, 2661, 2678
BtsI GCAGTG 1 cut(s) 892
BtsIMutI CAGTG 8 cut(s) 421, 473, 522, 892, 1465, 2385, 2895, 3093
Cac8I GCNNGC 3 cut(s) 1134, 1208, 1790
CaiI CAGNNNCTG 1 cut(s) 491
CfoI GCGC 1 cut(s) 537
Cfr13I GGNCC 4 cut(s) 106, 677, 1010, 1668
Csp6I GTAC 5 cut(s) 938, 2198, 2214, 2231, 2919
CviQI GTAC 5 cut(s) 938, 2198, 2214, 2231, 2919
DdeI CTNAG 8 cut(s) 1322, 1712, 1928, 2310, 2417, 2646, 2854, 3081
DpnI GATC 8 cut(s) 209, 224, 290, 304, 348, 1566, 2316, 2444
DpnII GATC 8 cut(s) 207, 222, 288, 302, 346, 1564, 2314, 2442
DraI TTTAAA 2 cut(s) 126, 1806
EaeI YGGCCR 3 cut(s) 505, 612, 1533
Eam1104I CTCTTC 8 cut(s) 28, 207, 399, 1980, 2100, 2139, 2883, 3086
EarI CTCTTC 8 cut(s) 28, 207, 399, 1980, 2100, 2139, 2883, 3086
Ecl136II GAGCTC 2 cut(s) 1120, 1741
Eco130I CCWWGG 3 cut(s) 1664, 2099, 2241
Eco24I GRGCYC 2 cut(s) 1122, 1743
Eco32I GATATC 2 cut(s) 140, 1166
Eco47I GGWCC 3 cut(s) 677, 1010, 1668
Eco53kI GAGCTC 2 cut(s) 1120, 1741
Eco57I CTGAAG 6 cut(s) 58, 416, 1073, 1659, 1706, 1928
Eco81I CCTNAGG 1 cut(s) 2310
EcoICRI GAGCTC 2 cut(s) 1120, 1741
EcoNI CCTNNNNNAGG 1 cut(s) 1158
EcoRI GAATTC 1 cut(s) 776
EcoRII CCWGG 2 cut(s) 1012, 1570
EcoRV GATATC 2 cut(s) 140, 1166
EcoT14I CCWWGG 3 cut(s) 1664, 2099, 2241
EcoT22I ATGCAT 3 cut(s) 1138, 1515, 2482
EcoT38I GRGCYC 2 cut(s) 1122, 1743
ErhI CCWWGG 3 cut(s) 1664, 2099, 2241
FalI AAGNNNNNCTT 6 cut(s) 531, 563, 1152, 1184, 2041, 2073
FaqI GGGAC 2 cut(s) 3012, 3131
FbaI TGATCA 1 cut(s) 1564
Fnu4HI GCNGC 2 cut(s) 120, 492
FokI GGATG 9 cut(s) 13, 440, 724, 1407, 1443, 2160, 2436, 2648, 2685
FriOI GRGCYC 2 cut(s) 1122, 1743
Fsp4HI GCNGC 2 cut(s) 120, 492
FspBI CTAG 6 cut(s) 827, 848, 1214, 1343, 1844, 2276
GlaI GCGC 1 cut(s) 536
GluI GCNGC 2 cut(s) 120, 492
GsaI CCCAGC 1 cut(s) 2382
GsuI CTGGAG 2 cut(s) 332, 1136
HaeIII GGCC 5 cut(s) 108, 507, 614, 1153, 1535
HapII CCGG 3 cut(s) 202, 1958, 2586
HhaI GCGC 1 cut(s) 537
Hin6I GCGC 1 cut(s) 535
HinP1I GCGC 1 cut(s) 535
HincII GTYRAC 3 cut(s) 565, 2743, 2832
HindII GTYRAC 3 cut(s) 565, 2743, 2832
HindIII AAGCTT 3 cut(s) 545, 2084, 2432
HpaII CCGG 3 cut(s) 202, 1958, 2586
HphI GGTGA 8 cut(s) 295, 728, 1559, 1600, 1947, 2018, 2074, 2257
Hpy99I CGWCG 1 cut(s) 800
HpyCH4III ACNGT 7 cut(s) 198, 468, 736, 1489, 2055, 2899, 3088
HpyCH4IV ACGT 4 cut(s) 439, 798, 928, 2229
HpyF10VI GCNNNNNNNGC 5 cut(s) 105, 1519, 2379, 2850, 3025
HpyF3I CTNAG 8 cut(s) 1322, 1712, 1928, 2310, 2417, 2646, 2854, 3081
HpySE526I ACGT 4 cut(s) 439, 798, 928, 2229
HspAI GCGC 1 cut(s) 535
Ksp22I TGATCA 1 cut(s) 1564
Kzo9I GATC 8 cut(s) 207, 222, 288, 302, 346, 1564, 2314, 2442
LguI GCTCTTC 1 cut(s) 1980
LmnI GCTCC 3 cut(s) 277, 313, 1117
Lsp1109I GCAGC 1 cut(s) 478
LweI GCATC 3 cut(s) 420, 1466, 2414
MaeI CTAG 6 cut(s) 827, 848, 1214, 1343, 1844, 2276
MaeII ACGT 4 cut(s) 439, 798, 928, 2229
MaeIII GTNAC 3 cut(s) 1483, 1606, 2078
MalI GATC 8 cut(s) 209, 224, 290, 304, 348, 1566, 2316, 2444
MboI GATC 8 cut(s) 207, 222, 288, 302, 346, 1564, 2314, 2442
MfeI CAATTG 2 cut(s) 1274, 2288
MflI RGATCY 3 cut(s) 222, 302, 2314
MhlI GDGCHC 3 cut(s) 1122, 1743, 2633
MlsI TGGCCA 3 cut(s) 507, 614, 1535
MluNI TGGCCA 3 cut(s) 507, 614, 1535
MlyI GAGTC 6 cut(s) 224, 1653, 2125, 2332, 2800, 2827
MmeI TCCRAC 2 cut(s) 607, 679
Mox20I TGGCCA 3 cut(s) 507, 614, 1535
Mph1103I ATGCAT 3 cut(s) 1138, 1515, 2482
MroXI GAANNNNTTC 4 cut(s) 390, 634, 1293, 2258
MscI TGGCCA 3 cut(s) 507, 614, 1535
Msp20I TGGCCA 3 cut(s) 507, 614, 1535
MspA1I CMGCKG 3 cut(s) 78, 2542, 2645
MspI CCGG 3 cut(s) 202, 1958, 2586
MspR9I CCNGG 3 cut(s) 203, 1014, 1572
MunI CAATTG 2 cut(s) 1274, 2288
Mva1269I GAATGC 2 cut(s) 642, 2006
MvaI CCWGG 2 cut(s) 1014, 1572
MvnI CGCG 2 cut(s) 119, 537
MwoI GCNNNNNNNGC 5 cut(s) 105, 1519, 2379, 2850, 3025
NciI CCSGG 1 cut(s) 203
NcoI CCATGG 1 cut(s) 2241
NdeII GATC 8 cut(s) 207, 222, 288, 302, 346, 1564, 2314, 2442
NlaIV GGNNCC 3 cut(s) 1595, 1894, 2584
NmuCI GTSAC 1 cut(s) 1606
NsiI ATGCAT 3 cut(s) 1138, 1515, 2482
NspI RCATGY 4 cut(s) 323, 359, 1136, 1861
NspV TTCGAA 2 cut(s) 1335, 2353
PaeI GCATGC 1 cut(s) 1136
PciI ACATGT 2 cut(s) 319, 355
PciSI GCTCTTC 1 cut(s) 1980
PcsI WCGNNNNNNNCGW 1 cut(s) 159
PctI GAATGC 2 cut(s) 642, 2006
PdmI GAANNNNTTC 4 cut(s) 390, 634, 1293, 2258
PfeI GAWTC 9 cut(s) 257, 630, 1455, 1797, 2023, 2347, 2555, 2865, 3068
PflFI GACNNNGTC 1 cut(s) 1409
PflMI CCANNNNNTGG 1 cut(s) 2610
PfoI TCCNGGA 1 cut(s) 1012
PkrI GCNGC 2 cut(s) 121, 493
Ple19I CGATCG 1 cut(s) 210
PleI GAGTC 6 cut(s) 223, 1652, 2125, 2331, 2800, 2827
PpsI GAGTC 6 cut(s) 223, 1652, 2125, 2331, 2800, 2827
Ppu21I YACGTR 1 cut(s) 2230
PscI ACATGT 2 cut(s) 319, 355
PshBI ATTAAT 2 cut(s) 1830, 1923
PsiI TTATAA 1 cut(s) 753
Psp124BI GAGCTC 2 cut(s) 1122, 1743
Psp6I CCWGG 2 cut(s) 1012, 1570
PspFI CCCAGC 1 cut(s) 2378
PspGI CCWGG 2 cut(s) 1012, 1570
PspN4I GGNNCC 3 cut(s) 1595, 1894, 2584
PspPI GGNCC 4 cut(s) 106, 677, 1010, 1668
PstNI CAGNNNCTG 1 cut(s) 491
PsuI RGATCY 3 cut(s) 222, 302, 2314
PsyI GACNNNGTC 1 cut(s) 1409
PvuI CGATCG 1 cut(s) 210
PvuII CAGCTG 2 cut(s) 2542, 2645
RsaI GTAC 5 cut(s) 939, 2199, 2215, 2232, 2920
RsaNI GTAC 5 cut(s) 938, 2198, 2214, 2231, 2919
SacI GAGCTC 2 cut(s) 1122, 1743
SapI GCTCTTC 1 cut(s) 1980
SatI GCNGC 2 cut(s) 120, 492
Sau3AI GATC 8 cut(s) 207, 222, 288, 302, 346, 1564, 2314, 2442
Sau96I GGNCC 4 cut(s) 106, 677, 1010, 1668
ScaI AGTACT 1 cut(s) 2199
SchI GAGTC 6 cut(s) 224, 1653, 2125, 2332, 2800, 2827
ScrFI CCNGG 3 cut(s) 203, 1014, 1572
SduI GDGCHC 3 cut(s) 1122, 1743, 2633
SfaNI GCATC 3 cut(s) 420, 1466, 2414
SfcI CTRYAG 1 cut(s) 2666
SfuI TTCGAA 2 cut(s) 1335, 2353
SinI GGWCC 3 cut(s) 677, 1010, 1668
SmlI CTYRAG 2 cut(s) 272, 1599
SmoI CTYRAG 2 cut(s) 272, 1599
SpeI ACTAGT 2 cut(s) 847, 1843
SphI GCATGC 1 cut(s) 1136
SsiI CCGC 6 cut(s) 78, 119, 254, 1085, 1200, 3065
SspI AATATT 2 cut(s) 333, 652
SspMI CTAG 6 cut(s) 827, 848, 1214, 1343, 1844, 2276
SstI GAGCTC 2 cut(s) 1122, 1743
StyD4I CCNGG 3 cut(s) 201, 1012, 1570
StyI CCWWGG 3 cut(s) 1664, 2099, 2241
TaaI ACNGT 7 cut(s) 198, 468, 736, 1489, 2055, 2899, 3088
TaiI ACGT 4 cut(s) 442, 801, 931, 2232
TatI WGTACW 3 cut(s) 2197, 2213, 2918
TauI GCSGC 1 cut(s) 122
TfiI GAWTC 9 cut(s) 257, 630, 1455, 1797, 2023, 2347, 2555, 2865, 3068
TscAI CASTG 8 cut(s) 421, 473, 529, 892, 1465, 2392, 2902, 3093
TseFI GTSAC 1 cut(s) 1606
TseI GCWGC 1 cut(s) 491
Tsp45I GTSAC 1 cut(s) 1606
TspGWI ACGGA 2 cut(s) 387, 2696
TspRI CASTG 8 cut(s) 421, 473, 529, 892, 1465, 2392, 2902, 3093
Tth111I GACNNNGTC 1 cut(s) 1409
Van91I CCANNNNNTGG 1 cut(s) 2610
VneI GTGCAC 1 cut(s) 2629
VpaK11BI GGWCC 3 cut(s) 677, 1010, 1668
VspI ATTAAT 2 cut(s) 1830, 1923
XagI CCTNNNNNAGG 1 cut(s) 1158
XapI RAATTY 9 cut(s) 496, 702, 759, 776, 1293, 1436, 2257, 2461, 2468
XceI RCATGY 4 cut(s) 323, 359, 1136, 1861
XmnI GAANNNNTTC 4 cut(s) 390, 634, 1293, 2258
XspI CTAG 6 cut(s) 827, 848, 1214, 1343, 1844, 2276
ZrmI AGTACT 1 cut(s) 2199
Zsp2I ATGCAT 3 cut(s) 1138, 1515, 2482
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.