TMV resistance protein N-like
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 3 cut(s) | 741, 1013, 2762 |
| Acc65I | GGTACC | 1 cut(s) | 609 |
| AccB1I | GGYRCC | 3 cut(s) | 609, 1476, 3419 |
| AccBSI | CCGCTC | 1 cut(s) | 2577 |
| AccI | GTMKAC | 1 cut(s) | 1500 |
| AccII | CGCG | 2 cut(s) | 521, 3309 |
| AciI | CCGC | 8 cut(s) | 519, 1193, 1434, 1552, 1729, 2577, 3410, 3701 |
| AclI | AACGTT | 1 cut(s) | 168 |
| AclWI | GGATC | 5 cut(s) | 362, 890, 1605, 2247, 3337 |
| AcoI | YGGCCR | 1 cut(s) | 2674 |
| AcuI | CTGAAG | 5 cut(s) | 82, 494, 897, 1428, 1701 |
| AfaI | GTAC | 7 cut(s) | 80, 472, 611, 921, 1258, 1352, 1914 |
| AfeI | AGCGCT | 1 cut(s) | 431 |
| AfiI | CCNNNNNNNGG | 6 cut(s) | 142, 525, 677, 2872, 3088, 3196 |
| AflIII | ACRYGT | 2 cut(s) | 1030, 2848 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 2992 |
| AhlI | ACTAGT | 1 cut(s) | 1657 |
| AjiI | CACGTC | 1 cut(s) | 591 |
| AjnI | CCWGG | 4 cut(s) | 994, 1464, 2470, 3397 |
| AleI | CACNNNNGTG | 1 cut(s) | 3691 |
| Alw21I | GWGCWC | 2 cut(s) | 1104, 2525 |
| Alw26I | GTCTC | 5 cut(s) | 442, 1036, 3000, 3266, 3368 |
| Alw44I | GTGCAC | 1 cut(s) | 2521 |
| AlwI | GGATC | 5 cut(s) | 362, 890, 1605, 2247, 3337 |
| AlwNI | CAGNNNCTG | 2 cut(s) | 2175, 2209 |
| Aor51HI | AGCGCT | 1 cut(s) | 431 |
| AoxI | GGCC | 2 cut(s) | 2086, 2674 |
| ApaLI | GTGCAC | 1 cut(s) | 2521 |
| ApeKI | GCWGC | 6 cut(s) | 184, 485, 2493, 2819, 3366, 3562 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 2717, 2749 |
| Asp700I | GAANNNNTTC | 6 cut(s) | 555, 1032, 1364, 1776, 2505, 3682 |
| Asp718I | GGTACC | 1 cut(s) | 609 |
| AspLEI | GCGC | 2 cut(s) | 432, 3311 |
| AspS9I | GGNCC | 5 cut(s) | 1999, 2323, 2912, 3239, 3728 |
| AsuHPI | GGTGA | 8 cut(s) | 116, 316, 1343, 1959, 2091, 2750, 2894, 3649 |
| AsuNHI | GCTAGC | 2 cut(s) | 1195, 2842 |
| AvaII | GGWCC | 5 cut(s) | 1999, 2323, 2912, 3239, 3728 |
| AxyI | CCTNAGG | 2 cut(s) | 1908, 2202 |
| BaeGI | GKGCMC | 2 cut(s) | 531, 2525 |
| BanI | GGYRCC | 3 cut(s) | 609, 1476, 3419 |
| BanII | GRGCYC | 1 cut(s) | 1104 |
| BarI | GAAGNNNNNNTAC | 4 cut(s) | 71, 103, 460, 492 |
| BauI | CACGAG | 2 cut(s) | 630, 1020 |
| BbsI | GAAGAC | 7 cut(s) | 79, 114, 1689, 2145, 3021, 3129, 3529 |
| Bbv12I | GWGCWC | 2 cut(s) | 1104, 2525 |
| BbvCI | CCTCAGC | 1 cut(s) | 2276 |
| BbvI | GCAGC | 6 cut(s) | 196, 472, 2480, 2806, 3353, 3549 |
| BccI | CCATC | 9 cut(s) | 758, 1328, 1421, 2078, 2110, 2459, 2559, 3320, 3481 |
| BceAI | ACGGC | 4 cut(s) | 159, 2661, 3231, 3329 |
| BciT130I | CCWGG | 4 cut(s) | 996, 1466, 2472, 3399 |
| BclI | TGATCA | 1 cut(s) | 673 |
| BcoDI | GTCTC | 5 cut(s) | 442, 1036, 3000, 3266, 3368 |
| BcuI | ACTAGT | 1 cut(s) | 1657 |
| BfmI | CTRYAG | 1 cut(s) | 3563 |
| BfoI | RGCGCY | 1 cut(s) | 433 |
| BglII | AGATCT | 1 cut(s) | 1936 |
| BisI | GCNGC | 9 cut(s) | 185, 486, 1193, 1435, 2494, 2820, 3367, 3411, 3563 |
| BlsI | GCNGC | 9 cut(s) | 186, 487, 1194, 1436, 2495, 2821, 3368, 3412, 3564 |
| BmcAI | AGTACT | 2 cut(s) | 472, 1352 |
| Bme1390I | CCNGG | 4 cut(s) | 996, 1466, 2472, 3399 |
| Bme18I | GGWCC | 5 cut(s) | 1999, 2323, 2912, 3239, 3728 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 2992 |
| BmgBI | CACGTC | 1 cut(s) | 591 |
| BmgT120I | GGNCC | 5 cut(s) | 1999, 2323, 2912, 3239, 3728 |
| BmiI | GGNNCC | 6 cut(s) | 611, 1478, 2469, 2913, 3296, 3421 |
| BmrFI | CCNGG | 4 cut(s) | 996, 1466, 2472, 3399 |
| BmrI | ACTGGG | 1 cut(s) | 2998 |
| BmsI | GCATC | 7 cut(s) | 199, 952, 2182, 2349, 2371, 2446, 3515 |
| BmtI | GCTAGC | 2 cut(s) | 1199, 2846 |
| BmuI | ACTGGG | 1 cut(s) | 2998 |
| BpiI | GAAGAC | 7 cut(s) | 79, 114, 1689, 2145, 3021, 3129, 3529 |
| BplI | GAGNNNNNCTC | 4 cut(s) | 457, 489, 1133, 1165 |
| BpmI | CTGGAG | 3 cut(s) | 1118, 1487, 3336 |
| Bpu10I | CCTNAGC | 1 cut(s) | 2276 |
| BpuEI | CTTGAG | 4 cut(s) | 112, 1871, 1937, 2015 |
| BsaBI | GATNNNNATC | 2 cut(s) | 366, 1630 |
| BsaJI | CCNNGG | 3 cut(s) | 995, 2907, 3724 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 3454, 3484 |
| Bsc4I | CCNNNNNNNGG | 6 cut(s) | 142, 525, 677, 2872, 3088, 3196 |
| Bse1I | ACTGG | 7 cut(s) | 222, 397, 1101, 1348, 1765, 2624, 2993 |
| Bse21I | CCTNAGG | 2 cut(s) | 1908, 2202 |
| Bse3DI | GCAATG | 2 cut(s) | 2812, 3376 |
| Bse8I | GATNNNNATC | 2 cut(s) | 366, 1630 |
| BseBI | CCWGG | 4 cut(s) | 996, 1466, 2472, 3399 |
| BseDI | CCNNGG | 3 cut(s) | 995, 2907, 3724 |
| BseGI | GGATG | 9 cut(s) | 47, 53, 301, 1432, 1559, 2089, 2102, 2570, 3506 |
| BseJI | GATNNNNATC | 2 cut(s) | 366, 1630 |
| BseLI | CCNNNNNNNGG | 6 cut(s) | 142, 525, 677, 2872, 3088, 3196 |
| BseMI | GCAATG | 2 cut(s) | 2812, 3376 |
| BseNI | ACTGG | 7 cut(s) | 222, 397, 1101, 1348, 1765, 2624, 2993 |
| BseRI | GAGGAG | 1 cut(s) | 1419 |
| BseSI | GKGCMC | 2 cut(s) | 531, 2525 |
| BseXI | GCAGC | 6 cut(s) | 196, 472, 2480, 2806, 3353, 3549 |
| BseYI | CCCAGC | 1 cut(s) | 2772 |
| BsgI | GTGCAG | 3 cut(s) | 242, 2365, 3523 |
| Bsh1236I | CGCG | 2 cut(s) | 521, 3309 |
| BshFI | GGCC | 2 cut(s) | 2088, 2676 |
| BshNI | GGYRCC | 3 cut(s) | 609, 1476, 3419 |
| BsiHKAI | GWGCWC | 2 cut(s) | 1104, 2525 |
| BslFI | GGGAC | 6 cut(s) | 601, 1544, 2925, 2972, 3412, 3476 |
| BslI | CCNNNNNNNGG | 6 cut(s) | 142, 525, 677, 2872, 3088, 3196 |
| BsmAI | GTCTC | 5 cut(s) | 442, 1036, 3000, 3266, 3368 |
| BsmBI | CGTCTC | 1 cut(s) | 442 |
| BsmFI | GGGAC | 6 cut(s) | 601, 1544, 2925, 2972, 3412, 3476 |
| BsmI | GAATGC | 4 cut(s) | 160, 1401, 1945, 2403 |
| BsnI | GGCC | 2 cut(s) | 2088, 2676 |
| Bsp1286I | GDGCHC | 3 cut(s) | 531, 1104, 2525 |
| BspACI | CCGC | 8 cut(s) | 519, 1193, 1434, 1552, 1729, 2577, 3410, 3701 |
| BspANI | GGCC | 2 cut(s) | 2088, 2676 |
| BspFNI | CGCG | 2 cut(s) | 521, 3309 |
| BspLI | GGNNCC | 6 cut(s) | 611, 1478, 2469, 2913, 3296, 3421 |
| BspMAI | CTGCAG | 1 cut(s) | 3567 |
| BspOI | GCTAGC | 2 cut(s) | 1199, 2846 |
| BspPI | GGATC | 5 cut(s) | 362, 890, 1605, 2247, 3337 |
| BspQI | GCTCTTC | 1 cut(s) | 420 |
| BspT107I | GGYRCC | 3 cut(s) | 609, 1476, 3419 |
| BsrBI | CCGCTC | 1 cut(s) | 2577 |
| BsrDI | GCAATG | 2 cut(s) | 2812, 3376 |
| BsrI | ACTGG | 7 cut(s) | 222, 397, 1101, 1348, 1765, 2624, 2993 |
| BssECI | CCNNGG | 3 cut(s) | 995, 2907, 3724 |
| BssSI | CACGAG | 2 cut(s) | 630, 1020 |
| BssT1I | CCWWGG | 2 cut(s) | 2907, 3724 |
| Bst2BI | CACGAG | 2 cut(s) | 630, 1020 |
| Bst2UI | CCWGG | 4 cut(s) | 996, 1466, 2472, 3399 |
| Bst4CI | ACNGT | 6 cut(s) | 724, 1181, 2018, 2698, 3294, 3693 |
| Bst6I | CTCTTC | 3 cut(s) | 64, 187, 420 |
| BstAPI | GCANNNNNTGC | 3 cut(s) | 1726, 3419, 3589 |
| BstC8I | GCNNGC | 8 cut(s) | 225, 1116, 1197, 1554, 1805, 1883, 2844, 3567 |
| BstF5I | GGATG | 9 cut(s) | 47, 53, 301, 1432, 1559, 2089, 2102, 2570, 3506 |
| BstFNI | CGCG | 2 cut(s) | 521, 3309 |
| BstH2I | RGCGCY | 1 cut(s) | 433 |
| BstHHI | GCGC | 2 cut(s) | 432, 3311 |
| BstMAI | GTCTC | 5 cut(s) | 442, 1036, 3000, 3266, 3368 |
| BstMWI | GCNNNNNNNGC | 7 cut(s) | 811, 1726, 1804, 2894, 3344, 3419, 3589 |
| BstNI | CCWGG | 4 cut(s) | 996, 1466, 2472, 3399 |
| BstNSI | RCATGY | 6 cut(s) | 1034, 1118, 1873, 2852, 2901, 3482 |
| BstSCI | CCNGG | 4 cut(s) | 994, 1464, 2470, 3397 |
| BstSFI | CTRYAG | 1 cut(s) | 3563 |
| BstSLI | GKGCMC | 2 cut(s) | 531, 2525 |
| BstUI | CGCG | 2 cut(s) | 521, 3309 |
| BstV1I | GCAGC | 6 cut(s) | 196, 472, 2480, 2806, 3353, 3549 |
| BstV2I | GAAGAC | 7 cut(s) | 79, 114, 1689, 2145, 3021, 3129, 3529 |
| BstX2I | RGATCY | 2 cut(s) | 1936, 2239 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 2988 |
| BstYI | RGATCY | 2 cut(s) | 1936, 2239 |
| Bsu36I | CCTNAGG | 2 cut(s) | 1908, 2202 |
| BsuRI | GGCC | 2 cut(s) | 2088, 2676 |
| BtrI | CACGTC | 1 cut(s) | 591 |
| BtsCI | GGATG | 9 cut(s) | 47, 53, 301, 1432, 1559, 2089, 2102, 2570, 3506 |
| BtsI | GCAGTG | 4 cut(s) | 800, 2377, 2382, 3420 |
| Cac8I | GCNNGC | 8 cut(s) | 225, 1116, 1197, 1554, 1805, 1883, 2844, 3567 |
| CaiI | CAGNNNCTG | 2 cut(s) | 2175, 2209 |
| CfoI | GCGC | 2 cut(s) | 432, 3311 |
| Cfr13I | GGNCC | 5 cut(s) | 1999, 2323, 2912, 3239, 3728 |
| CseI | GACGC | 1 cut(s) | 2894 |
| CsiI | ACCWGGT | 1 cut(s) | 3397 |
| Csp6I | GTAC | 7 cut(s) | 79, 471, 610, 920, 1257, 1351, 1913 |
| CviQI | GTAC | 7 cut(s) | 79, 471, 610, 920, 1257, 1351, 1913 |
| DriI | GACNNNNNGTC | 1 cut(s) | 2992 |
| EaeI | YGGCCR | 1 cut(s) | 2674 |
| Eam1104I | CTCTTC | 3 cut(s) | 64, 187, 420 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 2992 |
| EarI | CTCTTC | 3 cut(s) | 64, 187, 420 |
| EciI | GGCGGA | 1 cut(s) | 3690 |
| Ecl136II | GAGCTC | 1 cut(s) | 1102 |
| Eco130I | CCWWGG | 2 cut(s) | 2907, 3724 |
| Eco24I | GRGCYC | 1 cut(s) | 1104 |
| Eco32I | GATATC | 2 cut(s) | 2300, 2863 |
| Eco47I | GGWCC | 5 cut(s) | 1999, 2323, 2912, 3239, 3728 |
| Eco47III | AGCGCT | 1 cut(s) | 431 |
| Eco53kI | GAGCTC | 1 cut(s) | 1102 |
| Eco57I | CTGAAG | 5 cut(s) | 82, 494, 897, 1428, 1701 |
| Eco81I | CCTNAGG | 2 cut(s) | 1908, 2202 |
| EcoICRI | GAGCTC | 1 cut(s) | 1102 |
| EcoO109I | RGGNCCY | 4 cut(s) | 1999, 2323, 3239, 3728 |
| EcoRI | GAATTC | 2 cut(s) | 1145, 1455 |
| EcoRII | CCWGG | 4 cut(s) | 994, 1464, 2470, 3397 |
| EcoRV | GATATC | 2 cut(s) | 2300, 2863 |
| EcoT14I | CCWWGG | 2 cut(s) | 2907, 3724 |
| EcoT22I | ATGCAT | 3 cut(s) | 1518, 1722, 2364 |
| EcoT38I | GRGCYC | 1 cut(s) | 1104 |
| ErhI | CCWWGG | 2 cut(s) | 2907, 3724 |
| Esp3I | CGTCTC | 1 cut(s) | 442 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 2820, 2852 |
| FaqI | GGGAC | 6 cut(s) | 601, 1544, 2925, 2972, 3412, 3476 |
| FauI | CCCGC | 1 cut(s) | 1545 |
| FauNDI | CATATG | 4 cut(s) | 2415, 2422, 2511, 2792 |
| FbaI | TGATCA | 1 cut(s) | 673 |
| FblI | GTMKAC | 1 cut(s) | 1500 |
| Fnu4HI | GCNGC | 9 cut(s) | 185, 486, 1193, 1435, 2494, 2820, 3367, 3411, 3563 |
| FokI | GGATG | 9 cut(s) | 34, 40, 308, 1439, 1546, 2089, 2096, 2577, 3493 |
| FriOI | GRGCYC | 1 cut(s) | 1104 |
| Fsp4HI | GCNGC | 9 cut(s) | 185, 486, 1193, 1435, 2494, 2820, 3367, 3411, 3563 |
| GlaI | GCGC | 2 cut(s) | 431, 3310 |
| GluI | GCNGC | 9 cut(s) | 185, 486, 1193, 1435, 2494, 2820, 3367, 3411, 3563 |
| GsaI | CCCAGC | 1 cut(s) | 2776 |
| GsuI | CTGGAG | 3 cut(s) | 1118, 1487, 3336 |
| HaeII | RGCGCY | 1 cut(s) | 433 |
| HaeIII | GGCC | 2 cut(s) | 2088, 2676 |
| HgaI | GACGC | 1 cut(s) | 2894 |
| HhaI | GCGC | 2 cut(s) | 432, 3311 |
| Hin6I | GCGC | 2 cut(s) | 430, 3309 |
| HinP1I | GCGC | 2 cut(s) | 430, 3309 |
| HincII | GTYRAC | 4 cut(s) | 13, 2038, 3103, 3211 |
| HindII | GTYRAC | 4 cut(s) | 13, 2038, 3103, 3211 |
| HphI | GGTGA | 8 cut(s) | 116, 316, 1343, 1959, 2091, 2750, 2894, 3649 |
| Hpy166II | GTNNAC | 8 cut(s) | 13, 1501, 2038, 2099, 2523, 3103, 3211, 3462 |
| Hpy8I | GTNNAC | 8 cut(s) | 13, 1501, 2038, 2099, 2523, 3103, 3211, 3462 |
| HpyCH4III | ACNGT | 6 cut(s) | 724, 1181, 2018, 2698, 3294, 3693 |
| HpyCH4IV | ACGT | 4 cut(s) | 168, 590, 939, 1582 |
| HpyF10VI | GCNNNNNNNGC | 7 cut(s) | 811, 1726, 1804, 2894, 3344, 3419, 3589 |
| HpySE526I | ACGT | 4 cut(s) | 168, 590, 939, 1582 |
| HspAI | GCGC | 2 cut(s) | 430, 3309 |
| KpnI | GGTACC | 1 cut(s) | 613 |
| Ksp22I | TGATCA | 1 cut(s) | 673 |
| LguI | GCTCTTC | 1 cut(s) | 420 |
| LmnI | GCTCC | 4 cut(s) | 334, 997, 1099, 1740 |
| Lsp1109I | GCAGC | 6 cut(s) | 196, 472, 2480, 2806, 3353, 3549 |
| LweI | GCATC | 7 cut(s) | 199, 952, 2182, 2349, 2371, 2446, 3515 |
| MabI | ACCWGGT | 1 cut(s) | 3397 |
| MaeII | ACGT | 4 cut(s) | 168, 590, 939, 1582 |
| MaeIII | GTNAC | 5 cut(s) | 1952, 2809, 2882, 3065, 3173 |
| MbiI | CCGCTC | 1 cut(s) | 2577 |
| MfeI | CAATTG | 2 cut(s) | 2314, 3453 |
| MflI | RGATCY | 2 cut(s) | 1936, 2239 |
| MhlI | GDGCHC | 3 cut(s) | 531, 1104, 2525 |
| MlyI | GAGTC | 4 cut(s) | 2720, 3002, 3434, 3490 |
| MmeI | TCCRAC | 5 cut(s) | 256, 598, 643, 1762, 3622 |
| Mph1103I | ATGCAT | 3 cut(s) | 1518, 1722, 2364 |
| MroXI | GAANNNNTTC | 6 cut(s) | 555, 1032, 1364, 1776, 2505, 3682 |
| MslI | CAYNNNNRTG | 3 cut(s) | 1605, 2501, 3691 |
| MspR9I | CCNGG | 4 cut(s) | 996, 1466, 2472, 3399 |
| MunI | CAATTG | 2 cut(s) | 2314, 3453 |
| Mva1269I | GAATGC | 4 cut(s) | 160, 1401, 1945, 2403 |
| MvaI | CCWGG | 4 cut(s) | 996, 1466, 2472, 3399 |
| MvnI | CGCG | 2 cut(s) | 521, 3309 |
| MwoI | GCNNNNNNNGC | 7 cut(s) | 811, 1726, 1804, 2894, 3344, 3419, 3589 |
| NdeI | CATATG | 4 cut(s) | 2415, 2422, 2511, 2792 |
| NheI | GCTAGC | 2 cut(s) | 1195, 2842 |
| NlaIV | GGNNCC | 6 cut(s) | 611, 1478, 2469, 2913, 3296, 3421 |
| NmeAIII | GCCGAG | 1 cut(s) | 3448 |
| NmuCI | GTSAC | 3 cut(s) | 2882, 3065, 3173 |
| NsiI | ATGCAT | 3 cut(s) | 1518, 1722, 2364 |
| NspI | RCATGY | 6 cut(s) | 1034, 1118, 1873, 2852, 2901, 3482 |
| OliI | CACNNNNGTG | 1 cut(s) | 3691 |
| PaeI | GCATGC | 1 cut(s) | 1118 |
| PciI | ACATGT | 2 cut(s) | 1030, 2848 |
| PciSI | GCTCTTC | 1 cut(s) | 420 |
| PctI | GAATGC | 4 cut(s) | 160, 1401, 1945, 2403 |
| PdmI | GAANNNNTTC | 6 cut(s) | 555, 1032, 1364, 1776, 2505, 3682 |
| PfeI | GAWTC | 8 cut(s) | 278, 626, 1264, 1626, 2108, 2125, 2506, 3707 |
| PfoI | TCCNGGA | 1 cut(s) | 1464 |
| PkrI | GCNGC | 9 cut(s) | 186, 487, 1194, 1436, 2495, 2821, 3368, 3412, 3564 |
| PleI | GAGTC | 4 cut(s) | 2720, 3001, 3433, 3490 |
| PpsI | GAGTC | 4 cut(s) | 2720, 3001, 3433, 3490 |
| PpuMI | RGGWCCY | 4 cut(s) | 1999, 2323, 3239, 3728 |
| PscI | ACATGT | 2 cut(s) | 1030, 2848 |
| PsiI | TTATAA | 3 cut(s) | 741, 1013, 2762 |
| Psp124BI | GAGCTC | 1 cut(s) | 1104 |
| Psp1406I | AACGTT | 1 cut(s) | 168 |
| Psp5II | RGGWCCY | 4 cut(s) | 1999, 2323, 3239, 3728 |
| Psp6I | CCWGG | 4 cut(s) | 994, 1464, 2470, 3397 |
| PspFI | CCCAGC | 1 cut(s) | 2772 |
| PspGI | CCWGG | 4 cut(s) | 994, 1464, 2470, 3397 |
| PspN4I | GGNNCC | 6 cut(s) | 611, 1478, 2469, 2913, 3296, 3421 |
| PspPI | GGNCC | 5 cut(s) | 1999, 2323, 2912, 3239, 3728 |
| PspPPI | RGGWCCY | 4 cut(s) | 1999, 2323, 3239, 3728 |
| PstI | CTGCAG | 1 cut(s) | 3567 |
| PstNI | CAGNNNCTG | 2 cut(s) | 2175, 2209 |
| PsuI | RGATCY | 2 cut(s) | 1936, 2239 |
| RsaI | GTAC | 7 cut(s) | 80, 472, 611, 921, 1258, 1352, 1914 |
| RsaNI | GTAC | 7 cut(s) | 79, 471, 610, 920, 1257, 1351, 1913 |
| RseI | CAYNNNNRTG | 3 cut(s) | 1605, 2501, 3691 |
| SacI | GAGCTC | 1 cut(s) | 1104 |
| SapI | GCTCTTC | 1 cut(s) | 420 |
| SatI | GCNGC | 9 cut(s) | 185, 486, 1193, 1435, 2494, 2820, 3367, 3411, 3563 |
| Sau96I | GGNCC | 5 cut(s) | 1999, 2323, 2912, 3239, 3728 |
| ScaI | AGTACT | 2 cut(s) | 472, 1352 |
| SchI | GAGTC | 4 cut(s) | 2720, 3002, 3434, 3490 |
| ScrFI | CCNGG | 4 cut(s) | 996, 1466, 2472, 3399 |
| SduI | GDGCHC | 3 cut(s) | 531, 1104, 2525 |
| SexAI | ACCWGGT | 1 cut(s) | 3397 |
| SfaNI | GCATC | 7 cut(s) | 199, 952, 2182, 2349, 2371, 2446, 3515 |
| SfcI | CTRYAG | 1 cut(s) | 3563 |
| SinI | GGWCC | 5 cut(s) | 1999, 2323, 2912, 3239, 3728 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 1605, 2501, 3691 |
| SmlI | CTYRAG | 4 cut(s) | 91, 1886, 1916, 1994 |
| SmoI | CTYRAG | 4 cut(s) | 91, 1886, 1916, 1994 |
| SpeI | ACTAGT | 1 cut(s) | 1657 |
| SphI | GCATGC | 1 cut(s) | 1118 |
| SsiI | CCGC | 8 cut(s) | 519, 1193, 1434, 1552, 1729, 2577, 3410, 3701 |
| SspI | AATATT | 3 cut(s) | 354, 1294, 1422 |
| SstI | GAGCTC | 1 cut(s) | 1104 |
| StyD4I | CCNGG | 4 cut(s) | 994, 1464, 2470, 3397 |
| StyI | CCWWGG | 2 cut(s) | 2907, 3724 |
| TaaI | ACNGT | 6 cut(s) | 724, 1181, 2018, 2698, 3294, 3693 |
| TaiI | ACGT | 4 cut(s) | 171, 593, 942, 1585 |
| TaqI | TCGA | 3 cut(s) | 1072, 1866, 2010 |
| TatI | WGTACW | 3 cut(s) | 470, 1256, 1350 |
| TauI | GCSGC | 3 cut(s) | 1195, 1437, 3413 |
| TfiI | GAWTC | 8 cut(s) | 278, 626, 1264, 1626, 2108, 2125, 2506, 3707 |
| TseFI | GTSAC | 3 cut(s) | 2882, 3065, 3173 |
| TseI | GCWGC | 6 cut(s) | 184, 485, 2493, 2819, 3366, 3562 |
| Tsp45I | GTSAC | 3 cut(s) | 2882, 3065, 3173 |
| VneI | GTGCAC | 1 cut(s) | 2521 |
| VpaK11BI | GGWCC | 5 cut(s) | 1999, 2323, 2912, 3239, 3728 |
| XbaI | TCTAGA | 2 cut(s) | 1663, 1933 |
| XceI | RCATGY | 6 cut(s) | 1034, 1118, 1873, 2852, 2901, 3482 |
| XmiI | GTMKAC | 1 cut(s) | 1500 |
| XmnI | GAANNNNTTC | 6 cut(s) | 555, 1032, 1364, 1776, 2505, 3682 |
| ZrmI | AGTACT | 2 cut(s) | 472, 1352 |
| Zsp2I | ATGCAT | 3 cut(s) | 1518, 1722, 2364 |