Rroxscaffold_3G00226370

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0000055
Physical Location & Seq
Forward (+)
9885099 .. 9888716
3618 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00226370.1

Sequence Viewer

Length: 2463 bp
ATGAAGTCAACGGAGCCATCATCGTCATCATTCCAGCCGGGGCATTCATCGTCATCATCACCACTGGAGCCACCGTCCTCACTGTACTGGGACACATATTGGATAACCTACGACGTGTTCTTGAGCTTCAGAGGTGAAGACACACGCAGGACCTTCACGGACCACCTCTACGCGGCACTAATTCGTGCGGGAGTCAGCACGTTTTGGGATGCCGAGGGACTAAGAAGAGGGGAAAAAATATCAGAGGAATTGGGGCGGATAATCCAGGAGTGTAGGATCTCTCTAATTGTCTTCTCAGAGAAGTACGCGGATTCAAGTTGGTGTCTTGAGGAGCTGGTGCACATCATGGAGTGTAGAAAAACACGGGGGCAGTTGGTCTTCCCAATATTCTATCATGTTGATCCTTCTCATGTCAGAAACCAGGCCGGTAGTTTTGGTAAGGCGTTTGAGAAACATGAGTGGAGAACTCCAATTATAGAAGCAGCAATTATGGGACGAAGGGTTACTTTAGAATTATCTAGTGATGTACAAAAGAAATATGAAGCTAGACGTCATCATGGTACAGAAAAGATAGCCAAGTGGAGAGCTGCTCTTAAAGGAGCTGCAAATTTGGCCGGCTTCGATATCGCTGATAGGCCGGAAGCAGAGTTTATCAGTAGCATTGTTGACGAGATATGCAGACAGCTGACCACCCCATACTTGAATTTAGCTGTCTACCCAGTTGGAATTGATTCTCGTGTTGAAGATATTAGTAAATGCTTAGGTGTTGGATTAGATAATGATGTTCGCATGGTTGGAATTTGGGGTATGGGTGGAATTGGGAAAACAACAGTTGCCAAAGCCATCTATAACAAATTTTATCATAATTTTGACAGTAAAAGTTTCCTAGCAGATGTTAGGGAAACTGCAAAGGACTCAAATGGTAAGATTGCTCTGCAAGAAAGACTTCTTTCTGATGTCTTAAAACCAACCAAGATAGTGGTAGGTGATGTTTCTAGAGGGATCAATGTGATAAGAGAACGACTTGGAAGGAAAAAGGTACTTTTCATTGTTGATGATGTAGATCATGAGGACCAATTGAATGCATTGGCTATAAGGCGTGATTCCTTTGGTCCGGGAAGTAGAATTATTATAACAACAAGAGATCGACATTTGCTAGAACTACTGAAAGTGGATACAATACATCTGACCCAAGAAATGAATGAAGAAGAAGCTATTGAGCTCTTCAGTTGGCATGCCTTTCAAAATCATTCTCCTAATGAAGATTATATCGAACTCTCAAGAAGAGTGGTTACTTACTGTGGAGGTTTACCACTTGCCCTTGAAATTTTAGGGTCTTTCCTCTTTGGAAGGAGCATAGAAGATTGGATTAGCACACTGAAGAAATTGAAAAAAATTCCTGATGTCAGAATTCAGTCAAGGCTCAGAATAAGCTTTGATGCAATTGATGAGAGCCAGAGGAACATATTCCTCCATATATGTTGTTACTTCATCGGAATGGACAAGAACTATGTCATAAAAATACTCAAGGGATGTGGTTTTTCTCCAGAAATAGAAATCAGTGTCCTGCTCCAACGTTGCCTTGTAACTGTTAGTGAGAAAAACAAGCTCATGATGCATGATTTGCTTCGAGACATGGGCAGAGAAGTTGTTCGTGAAGAATCCCCAAACCGCCCTGAAAGACGTAGTAGATTGTGGCGTAAGGAAGATGTAATAGATGTGTTGACAGATGAATCTGGAACTGAAGAGACTGAAGGACTTGCTATAAATTTGCAAAGATCTGACAACATGAGTTTCAGTACAGAATCATTTAGAAACATGAAGAGACTGAAATTGCTCCAACTCAACCATGTACAACTCACTGGAGACTGCGACAAGTTTCCCAAAAAGTTAAGGTGGCTCTGCTTGCGAGGATTCTCTCTACAGTCCATTCGAAATGAGCTTCTTAATGAGACATACCTGGTTTCTATCGACCTGCGGTACAGCAATCTTGTACGAGTTTGGGAGTATTCCTGGCGGCTTGGGAAATTGGAGATACTTAATCTCAGTCATTCACATTACCTAAAACAATCACCAGACTTTTCACACCTCCCAAAGCTACAGTATTTGATCCTTAAAGACTGTGTGAGTTTGCCAGAGATTCACTGGTCGGTTGGACTTCTTCGGAGACTTGCCTTGCTAAATCTTAAAGGCTGCACCATGCTCAAGGACCTTCCGGAGGACTTTTATATGTTGATTCATGTTAAGACTCTTGTTCTTTCTGGCTGTTCGAGATTTGAGAATTTGAGCAAGAATATAGGAAATATGATCTCATTGAAAACTCTTATTATAAGTGGCACAGCCATAAGTGAAGTACCATCCTCTATAGATAACATGAGGAGACTGGACTTTTCATCTCGACAAGGCCTGAGTGGCCTAAAACGTTGTTTCCAGGATGAGACATCAGGACCAATCCGAGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

820

Amino Acids

93.97

Weight (kDa)

8.32

Isoelectric Point (pI)

55.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 37 - 153 6.3e-43 TIR domain
TIR_2 PF13676 39 - 135 1e-14 TIR domain
NB-ARC PF00931 259 - 417 1.2e-20 NB-ARC domain
WHD_ROQ1 PF23282 484 - 555 1.5e-18 Disease resistance protein Roq1-like, winged-helix domain
LRR_14 PF23598 724 - 795 5.8e-07 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000055)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G36930 AT5G36930 AT5G36930 AT5G36930
fragaria_vesca FvH4_3g45361 FvH4_3g45380 FvH4_5g21061 FvH4_5g21062 FvH4_5g21063 FvH4_5g32050 FvH4_5g32970 FvH4_5g32990 FvH4_5g34190 FvH4_5g34210 FvH4_5g34210 FvH4_5g34210 FvH4_5g34210 FvH4_5g34210 FvH4_5g34210 FvH4_5g34211 FvH4_5g34240 FvH4_5g34250
malus_domestica MD00G1011700.v1.1 MD00G1026100.v1.1 MD00G1026500.v1.1 MD00G1027000.v1.1 MD00G1027100.v1.1 MD00G1027700.v1.1 MD00G1027800.v1.1 MD00G1028000.v1.1 MD00G1220000.v1.1 MD02G1024400.v1.1 MD02G1024800.v1.1 MD02G1026000.v1.1 MD02G1026100.v1.1 MD02G1031800.v1.1 MD02G1038500.v1.1 MD02G1038700.v1.1 MD02G1039200.v1.1 MD02G1040600.v1.1 MD02G1040900.v1.1 MD02G1041200.v1.1 MD02G1041300.v1.1 MD02G1041700.v1.1 MD02G1042000.v1.1 MD02G1043800.v1.1 MD02G1043900.v1.1 MD02G1044300.v1.1 MD02G1045200.v1.1 MD02G1045300.v1.1 MD02G1051200.v1.1 MD02G1052200.v1.1 MD02G1052800.v1.1 MD02G1053100.v1.1 MD02G1053600.v1.1 MD02G1055500.v1.1 MD02G1063300.v1.1 MD02G1063400.v1.1 MD02G1063800.v1.1 MD02G1306500.v1.1 MD02G1306600.v1.1 MD02G1306700.v1.1 MD02G1306900.v1.1 MD02G1307200.v1.1 MD04G1011700.v1.1 MD05G1315400.v1.1 MD05G1316000.v1.1 MD05G1316700.v1.1 MD05G1317000.v1.1 MD05G1317300.v1.1 MD05G1317600.v1.1 MD05G1317700.v1.1 MD07G1102800.v1.1 MD07G1238200.v1.1 MD08G1175900.v1.1 MD08G1203600.v1.1 MD09G1024200.v1.1 MD09G1039600.v1.1 MD09G1044800.v1.1 MD09G1045100.v1.1 MD10G1038700.v1.1 MD10G1039000.v1.1 MD10G1039200.v1.1 MD10G1040000.v1.1 MD10G1040200.v1.1 MD10G1040600.v1.1 MD10G1040800.v1.1 MD10G1297300.v1.1 MD12G1247500.v1.1 MD12G1247900.v1.1 MD12G1248300.v1.1 MD12G1248800.v1.1 MD15G1103700.v1.1 MD15G1103800.v1.1 MD15G1104000.v1.1 MD15G1179600.v1.1 MD15G1182500.v1.1 MD16G1068200.v1.1 MD16G1068300.v1.1 MD17G1024600.v1.1 MD17G1024700.v1.1 MD17G1025000.v1.1 MD17G1025900.v1.1
prunus_persica Prupe.1G539800_v2.0.a1 Prupe.1G550800_v2.0.a1 Prupe.1G550800_v2.0.a1 Prupe.1G550800_v2.0.a1 Prupe.1G550800_v2.0.a1 Prupe.1G550800_v2.0.a1 Prupe.1G550800_v2.0.a1 Prupe.1G557100_v2.0.a1 Prupe.1G557200_v2.0.a1 Prupe.1G557200_v2.0.a1 Prupe.1G557300_v2.0.a1
pyrus_communis pycom02g02080 pycom02g02090 pycom02g02110 pycom02g03220 pycom02g03260 pycom02g03370 pycom02g03390 pycom02g03410 pycom02g03420 pycom02g03450 pycom02g03500 pycom02g03730 pycom02g03770 pycom02g04400 pycom02g04410 pycom02g04430 pycom02g04450 pycom02g04460 pycom02g04490 pycom02g05200 pycom02g25710 pycom02g25750 pycom02g25790 pycom02g25810 pycom02g25830 pycom04g00920 pycom04g00930 pycom04g00940 pycom05g29300 pycom05g29310 pycom05g29380 pycom05g29410 pycom07g06310 pycom07g21420 pycom08g15070 pycom08g17540 pycom08g17560 pycom08g17570 pycom10g02670 pycom10g02680 pycom10g02700 pycom10g02770 pycom10g02790 pycom10g02800 pycom10g02810 pycom10g25020 pycom10g25030 pycom10g25050 pycom10g25080 pycom111g01990 pycom111g02000 pycom111g02030 pycom111g02040 pycom111g02050 pycom111g02060 pycom12g22710 pycom12g22720 pycom12g22730 pycom12g22740 pycom12g22750 pycom12g22770 pycom12g22780 pycom12g22790 pycom12g22800 pycom12g22810 pycom12g22830 pycom12g22840 pycom12g22860 pycom15g09440 pycom15g09480 pycom15g16120 pycom15g32380 pycom16g05970 pycom17g02100
rosa_chinensis RchiOBHm_Chr2g0144661 RchiOBHm_Chr4g0407491 RchiOBHm_Chr4g0407501 RchiOBHm_Chr4g0412381 RchiOBHm_Chr7g0208191 RchiOBHm_Chr7g0208201 RchiOBHm_Chr7g0208211 RchiOBHm_Chr7g0208221 RchiOBHm_Chr7g0228071 RchiOBHm_Chr7g0228121 RchiOBHm_Chr7g0230971 RchiOBHm_Chr7g0230981 RchiOBHm_Chr7g0233901 RchiOBHm_Chr7g0233951 RchiOBHm_Chr7g0233961 RchiOBHm_Chr7g0233991 RchiOBHm_Chr7g0234021 RchiOBHm_Chr7g0234151 RchiOBHm_Chr7g0234161 RchiOBHm_Chr7g0234241 RchiOBHm_Chr7g0234501 RchiOBHm_Chr7g0234641 RchiOBHm_Chr7g0234771 RchiOBHm_Chr7g0234781 RchiOBHm_Chr7g0234991 RchiOBHm_Chr7g0241291 RchiOBHm_Chr7g0241301
rosa_laevigata RLG00000001186 RLG00000001187 RLG00000001212 RLG00000001226 RLG00000001233 RLG00000001590 RLG00000003242 RLG00000003243 RLG00000003249 RLG00000008688 RLG00000008690
rosa_multiflora Rmu_co7966574.1_g000001 Rmu_co8120830.1_g000001 Rmu_co8142226.1_g000001 Rmu_co8293083.1_g000001 Rmu_co8351227.1_g000001 Rmu_co8380159.1_g000001 Rmu_co8456505.1_g000001 Rmu_sc0000676.1_g000025 Rmu_sc0000706.1_g000018 Rmu_sc0000706.1_g000040 Rmu_sc0000740.1_g000029 Rmu_sc0000740.1_g000032 Rmu_sc0000740.1_g000037 Rmu_sc0000740.1_g000040 Rmu_sc0000762.1_g000003 Rmu_sc0000762.1_g000019 Rmu_sc0000762.1_g000034 Rmu_sc0000762.1_g000069 Rmu_sc0000892.1_g000001 Rmu_sc0000945.1_g000040 Rmu_sc0001913.1_g000037 Rmu_sc0001913.1_g000041 Rmu_sc0001913.1_g000049 Rmu_sc0002775.1_g000017 Rmu_sc0002775.1_g000018 Rmu_sc0002775.1_g000020 Rmu_sc0002775.1_g000021 Rmu_sc0002775.1_g000022 Rmu_sc0002775.1_g000025 Rmu_sc0002775.1_g000026 Rmu_sc0002775.1_g000028 Rmu_sc0002775.1_g000030 Rmu_sc0004858.1_g000002 Rmu_sc0004864.1_g000022 Rmu_sc0005038.1_g000023 Rmu_sc0005840.1_g000014 Rmu_sc0006724.1_g000003 Rmu_sc0006824.1_g000005 Rmu_sc0007869.1_g000003 Rmu_sc0007869.1_g000005 Rmu_sc0007869.1_g000008 Rmu_sc0008646.1_g000007 Rmu_sc0009637.1_g000009 Rmu_sc0010500.1_g000002 Rmu_sc0010500.1_g000012 Rmu_sc0012403.1_g000025 Rmu_sc0013768.1_g000025 Rmu_sc0015625.1_g000001 Rmu_sc0019715.1_g000001 Rmu_sc0021133.1_g000002 Rmu_sc0021904.1_g000001 Rmu_sc0021904.1_g000003 Rmu_sc0021904.1_g000004 Rmu_sc0022054.1_g000001 Rmu_sc0026025.1_g000001 Rmu_sc0036828.1_g000002 Rmu_sc0041808.1_g000001 Rmu_ssc0000012.1_g000001
rosa_roxburghii Rroxscaffold_3G00226370 Rroxscaffold_3G00226860 Rroxscaffold_3G00226900 Rroxscaffold_3G00226930 Rroxscaffold_3G00226940 Rroxscaffold_3G00226970 Rroxscaffold_3G00226980 Rroxscaffold_3G00231530 Rroxscaffold_3G00237090 Rroxscaffold_3G00250760 Rroxscaffold_3G00250800
rosa_rugosa Rorug04G0082300 Rorug07G0101800 Rorug07G0102000 Rorug07G0102100 Rorug07G0221200 Rorug07G0245700 Rorug07G0245700 Rorug07G0245700 Rorug07G0263900 Rorug07G0264000 Rorug07G0264000 Rorug07G0264500 Rorug07G0282900 Rorug07G0282900 Rorug07G0283100 Rorug07G0283200.1 Rorug07G0283300 Rorug07G0283900 Rorug07G0284000 Rorug07G0284100 Rorug07G0284300 Rorug07G0284400 Rorug07G0284500 Rorug07G0285000 Rorug07G0285200 Rorug07G0285300 Rorug07G0285500 Rorug07G0286800 Rorug07G0286900 Rorug07G0287100 Rorug07G0287200
rosa_samantha Rh7AG236100 Rh7AG236200 Rh7AG236300 Rh7AG440000 Rh7AG440100 Rh7BG411900 Rh7BG412000 Rh7BG412600 Rh7CG251300 Rh7CG417700 Rh7CG460100 Rh7DG242100 Rh7DG242200 Rh7DG242300 Rh7DG429300
rosa_wichuraiana Rw0G005070 Rw0G010780 Rw0G012530 Rw7G020110 Rw7G020160 Rw7G020170 Rw7G033210 Rw7G033230 Rw7G033250 Rw7G034540 Rw7G035150 Rw7G036190 Rw7G036210 Rw7G036260 Rw7G036270 Rw7G036290 Rw7G036300 Rw7G036310 Rw7G036350 Rw7G036360 Rw7G036440 Rw7G036510 Rw7G036530 Rw7G036570 Rw7G036590 Rw7G036730

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1133, 2330
AatII GACGTC 1 cut(s) 553
Acc36I ACCTGC 1 cut(s) 1983
AccI GTMKAC 1 cut(s) 714
AccII CGCG 2 cut(s) 173, 308
AccIII TCCGGA 1 cut(s) 2215
AciI CCGC 7 cut(s) 173, 188, 256, 308, 1672, 1978, 2017
AclI AACGTT 2 cut(s) 1576, 2422
AclWI GGATC 4 cut(s) 284, 395, 1010, 2104
AcoI YGGCCR 1 cut(s) 612
AcsI RAATTY 9 cut(s) 607, 703, 798, 854, 1326, 1395, 1410, 1768, 2281
AcuI CTGAAG 5 cut(s) 112, 1210, 1400, 1764, 1773
AcyI GRCGYC 1 cut(s) 550
AfiI CCNNNNNNNGG 3 cut(s) 172, 2218, 2456
AflIII ACRYGT 1 cut(s) 114
AgsI TTSAA 8 cut(s) 315, 703, 743, 1081, 1244, 1325, 1390, 2317
AjiI CACGTC 1 cut(s) 115
AjnI CCWGG 5 cut(s) 264, 420, 1959, 2012, 2430
Alw21I GWGCWC 2 cut(s) 342, 1224
Alw26I GTCTC 8 cut(s) 1626, 1742, 1819, 1860, 1946, 2161, 2374, 2432
Alw44I GTGCAC 1 cut(s) 338
AlwI GGATC 4 cut(s) 284, 395, 1010, 2104
Aor13HI TCCGGA 1 cut(s) 2215
AoxI GGCC 5 cut(s) 423, 612, 635, 2404, 2413
ApaLI GTGCAC 1 cut(s) 338
ApeKI GCWGC 4 cut(s) 482, 587, 602, 2193
ApoI RAATTY 9 cut(s) 607, 703, 798, 854, 1326, 1395, 1410, 1768, 2281
Asp700I GAANNNNTTC 4 cut(s) 730, 945, 1466, 1650
AspS9I GGNCC 6 cut(s) 150, 160, 1072, 1112, 2209, 2447
AsuC2I CCSGG 2 cut(s) 39, 1116
AsuHPI GGTGA 4 cut(s) 51, 146, 998, 2064
AsuII TTCGAA 1 cut(s) 1933
AvaII GGWCC 6 cut(s) 150, 160, 1072, 1112, 2209, 2447
BaeGI GKGCMC 1 cut(s) 342
BanII GRGCYC 1 cut(s) 1224
BarI GAAGNNNNNNTAC 2 cut(s) 1276, 1308
BauI CACGAG 1 cut(s) 735
BbsI GAAGAC 3 cut(s) 144, 283, 370
Bbv12I GWGCWC 2 cut(s) 342, 1224
BbvI GCAGC 4 cut(s) 494, 574, 589, 2180
BccI CCATC 3 cut(s) 25, 851, 2365
BciT130I CCWGG 5 cut(s) 266, 422, 1961, 2014, 2432
BciVI GTATCC 1 cut(s) 1168
BcnI CCSGG 2 cut(s) 39, 1116
BcoDI GTCTC 8 cut(s) 1626, 1742, 1819, 1860, 1946, 2161, 2374, 2432
BfaI CTAG 5 cut(s) 519, 546, 887, 996, 1157
BfmI CTRYAG 3 cut(s) 1922, 2099, 2364
BfuAI ACCTGC 1 cut(s) 1983
BfuI GTATCC 1 cut(s) 1168
BglI GCCNNNNNGGC 1 cut(s) 2412
BglII AGATCT 1 cut(s) 1778
BisI GCNGC 6 cut(s) 174, 483, 588, 603, 2018, 2194
BlsI GCNGC 6 cut(s) 175, 484, 589, 604, 2019, 2195
Bme1390I CCNGG 7 cut(s) 39, 266, 422, 1116, 1961, 2014, 2432
Bme18I GGWCC 6 cut(s) 150, 160, 1072, 1112, 2209, 2447
BmgBI CACGTC 1 cut(s) 115
BmgT120I GGNCC 6 cut(s) 150, 160, 1072, 1112, 2209, 2447
BmiI GGNNCC 2 cut(s) 15, 69
BmrFI CCNGG 7 cut(s) 39, 266, 422, 1116, 1961, 2014, 2432
BmrI ACTGGG 2 cut(s) 97, 713
BmsI GCATC 3 cut(s) 199, 1429, 1605
BmuI ACTGGG 2 cut(s) 97, 713
BpiI GAAGAC 3 cut(s) 144, 283, 370
BplI GAGNNNNNCTC 2 cut(s) 574, 606
BpmI CTGGAG 3 cut(s) 86, 1530, 1884
Bpu10I CCTNAGC 1 cut(s) 760
Bpu14I TTCGAA 1 cut(s) 1933
BpuEI CTTGAG 5 cut(s) 142, 347, 1264, 1511, 2189
BpuMI CCSGG 2 cut(s) 39, 1116
BsaBI GATNNNNATC 1 cut(s) 1062
BsaHI GRCGYC 1 cut(s) 550
BsaJI CCNNGG 3 cut(s) 38, 213, 2455
BsaWI WCCGGW 1 cut(s) 2215
BsaXI ACNNNNNCTCC 2 cut(s) 59, 89
Bsc4I CCNNNNNNNGG 3 cut(s) 172, 2218, 2456
Bse118I RCCGGY 2 cut(s) 425, 614
Bse1I ACTGG 6 cut(s) 69, 92, 719, 1867, 2150, 2388
Bse8I GATNNNNATC 1 cut(s) 1062
BseAI TCCGGA 1 cut(s) 2215
BseBI CCWGG 5 cut(s) 266, 422, 1961, 2014, 2432
BseDI CCNNGG 3 cut(s) 38, 213, 2455
BseGI GGATG 4 cut(s) 214, 1538, 2357, 2440
BseJI GATNNNNATC 1 cut(s) 1062
BseLI CCNNNNNNNGG 3 cut(s) 172, 2218, 2456
BseMII CTCAG 4 cut(s) 309, 1438, 2059, 2399
BseNI ACTGG 6 cut(s) 69, 92, 719, 1867, 2150, 2388
BseRI GAGGAG 2 cut(s) 344, 2392
BseSI GKGCMC 1 cut(s) 342
BseXI GCAGC 4 cut(s) 494, 574, 589, 2180
BsgI GTGCAG 1 cut(s) 2179
Bsh1236I CGCG 2 cut(s) 173, 308
BshFI GGCC 5 cut(s) 425, 614, 637, 2406, 2415
BsiHKAI GWGCWC 2 cut(s) 342, 1224
BsiSI CCGG 6 cut(s) 38, 426, 615, 638, 1115, 2216
BslFI GGGAC 3 cut(s) 104, 231, 507
BslI CCNNNNNNNGG 3 cut(s) 172, 2218, 2456
BsmAI GTCTC 8 cut(s) 1626, 1742, 1819, 1860, 1946, 2161, 2374, 2432
BsmFI GGGAC 3 cut(s) 104, 231, 507
BsmI GAATGC 2 cut(s) 43, 1087
BsnI GGCC 5 cut(s) 425, 614, 637, 2406, 2415
Bsp119I TTCGAA 1 cut(s) 1933
Bsp1286I GDGCHC 2 cut(s) 342, 1224
Bsp13I TCCGGA 1 cut(s) 2215
Bsp1407I TGTACA 2 cut(s) 526, 1852
Bsp143I GATC 8 cut(s) 276, 400, 1002, 1063, 1144, 1778, 2109, 2307
BspACI CCGC 7 cut(s) 173, 188, 256, 308, 1672, 1978, 2017
BspANI GGCC 5 cut(s) 425, 614, 637, 2406, 2415
BspCNI CTCAG 4 cut(s) 308, 1437, 2058, 2400
BspEI TCCGGA 1 cut(s) 2215
BspFNI CGCG 2 cut(s) 173, 308
BspHI TCATGA 2 cut(s) 1066, 1611
BspLI GGNNCC 2 cut(s) 15, 69
BspMI ACCTGC 1 cut(s) 1983
BspPI GGATC 4 cut(s) 284, 395, 1010, 2104
BspQI GCTCTTC 1 cut(s) 1229
BspT104I TTCGAA 1 cut(s) 1933
BsrFI RCCGGY 2 cut(s) 425, 614
BsrGI TGTACA 2 cut(s) 526, 1852
BsrI ACTGG 6 cut(s) 69, 92, 719, 1867, 2150, 2388
BssAI RCCGGY 2 cut(s) 425, 614
BssECI CCNNGG 3 cut(s) 38, 213, 2455
BssMI GATC 8 cut(s) 276, 400, 1002, 1063, 1144, 1778, 2109, 2307
BssNI GRCGYC 1 cut(s) 550
BssSI CACGAG 1 cut(s) 735
Bst2BI CACGAG 1 cut(s) 735
Bst2UI CCWGG 5 cut(s) 266, 422, 1961, 2014, 2432
Bst4CI ACNGT 9 cut(s) 75, 84, 832, 875, 1301, 1591, 1926, 2103, 2123
Bst6I CTCTTC 5 cut(s) 220, 1229, 1279, 1740, 1817
BstACI GRCGYC 1 cut(s) 550
BstAPI GCANNNNNTGC 1 cut(s) 1624
BstAUI TGTACA 2 cut(s) 526, 1852
BstBI TTCGAA 1 cut(s) 1933
BstC8I GCNNGC 3 cut(s) 616, 1236, 1907
BstDEI CTNAG 6 cut(s) 221, 295, 760, 1424, 2045, 2408
BstENI CCTNNNNNAGG 1 cut(s) 2216
BstF5I GGATG 4 cut(s) 214, 1538, 2357, 2440
BstFNI CGCG 2 cut(s) 173, 308
BstKTI GATC 8 cut(s) 279, 403, 1005, 1066, 1147, 1781, 2112, 2310
BstMAI GTCTC 8 cut(s) 1626, 1742, 1819, 1860, 1946, 2161, 2374, 2432
BstMBI GATC 8 cut(s) 276, 400, 1002, 1063, 1144, 1778, 2109, 2307
BstMWI GCNNNNNNNGC 5 cut(s) 611, 1615, 1624, 1906, 2412
BstNI CCWGG 5 cut(s) 266, 422, 1961, 2014, 2432
BstNSI RCATGY 1 cut(s) 1238
BstSCI CCNGG 7 cut(s) 37, 264, 420, 1114, 1959, 2012, 2430
BstSFI CTRYAG 3 cut(s) 1922, 2099, 2364
BstSLI GKGCMC 1 cut(s) 342
BstUI CGCG 2 cut(s) 173, 308
BstV1I GCAGC 4 cut(s) 494, 574, 589, 2180
BstV2I GAAGAC 3 cut(s) 144, 283, 370
BstX2I RGATCY 2 cut(s) 276, 1778
BstXI CCANNNNNNTGG 1 cut(s) 979
BstYI RGATCY 2 cut(s) 276, 1778
BsuI GTATCC 1 cut(s) 1168
BsuRI GGCC 5 cut(s) 425, 614, 637, 2406, 2415
BtrI CACGTC 1 cut(s) 115
BtsCI GGATG 4 cut(s) 214, 1538, 2357, 2440
BtsIMutI CAGTG 6 cut(s) 62, 80, 1376, 1567, 1860, 2143
BveI ACCTGC 1 cut(s) 1983
Cac8I GCNNGC 3 cut(s) 616, 1236, 1907
CciI TCATGA 2 cut(s) 1066, 1611
Cfr10I RCCGGY 2 cut(s) 425, 614
Cfr13I GGNCC 6 cut(s) 150, 160, 1072, 1112, 2209, 2447
CsiI ACCWGGT 1 cut(s) 1959
CspCI CAANNNNNGTGG 4 cut(s) 1269, 1304, 1516, 1551
DdeI CTNAG 6 cut(s) 221, 295, 760, 1424, 2045, 2408
DpnI GATC 8 cut(s) 278, 402, 1004, 1065, 1146, 1780, 2111, 2309
DpnII GATC 8 cut(s) 276, 400, 1002, 1063, 1144, 1778, 2109, 2307
EaeI YGGCCR 1 cut(s) 612
Eam1104I CTCTTC 5 cut(s) 220, 1229, 1279, 1740, 1817
EarI CTCTTC 5 cut(s) 220, 1229, 1279, 1740, 1817
EciI GGCGGA 1 cut(s) 271
Ecl136II GAGCTC 1 cut(s) 1222
Eco147I AGGCCT 1 cut(s) 2406
Eco24I GRGCYC 1 cut(s) 1224
Eco32I GATATC 1 cut(s) 625
Eco47I GGWCC 6 cut(s) 150, 160, 1072, 1112, 2209, 2447
Eco53kI GAGCTC 1 cut(s) 1222
Eco57I CTGAAG 5 cut(s) 112, 1210, 1400, 1764, 1773
EcoICRI GAGCTC 1 cut(s) 1222
EcoNI CCTNNNNNAGG 1 cut(s) 2216
EcoO109I RGGNCCY 2 cut(s) 150, 2209
EcoRI GAATTC 1 cut(s) 1410
EcoRII CCWGG 5 cut(s) 264, 420, 1959, 2012, 2430
EcoRV GATATC 1 cut(s) 625
EcoT22I ATGCAT 2 cut(s) 1087, 1620
EcoT38I GRGCYC 1 cut(s) 1224
FalI AAGNNNNNCTT 4 cut(s) 490, 522, 930, 962
FaqI GGGAC 3 cut(s) 104, 231, 507
FauI CCCGC 1 cut(s) 181
FblI GTMKAC 1 cut(s) 714
Fnu4HI GCNGC 6 cut(s) 174, 483, 588, 603, 2018, 2194
FokI GGATG 4 cut(s) 221, 1545, 2344, 2447
FriOI GRGCYC 1 cut(s) 1224
Fsp4HI GCNGC 6 cut(s) 174, 483, 588, 603, 2018, 2194
FspBI CTAG 5 cut(s) 519, 546, 887, 996, 1157
GluI GCNGC 6 cut(s) 174, 483, 588, 603, 2018, 2194
GsuI CTGGAG 3 cut(s) 86, 1530, 1884
HaeIII GGCC 5 cut(s) 425, 614, 637, 2406, 2415
HapII CCGG 6 cut(s) 38, 426, 615, 638, 1115, 2216
Hin1I GRCGYC 1 cut(s) 550
HincII GTYRAC 3 cut(s) 9, 667, 1725
HindII GTYRAC 3 cut(s) 9, 667, 1725
HindIII AAGCTT 1 cut(s) 1432
HpaII CCGG 6 cut(s) 38, 426, 615, 638, 1115, 2216
HphI GGTGA 4 cut(s) 51, 146, 998, 2064
Hpy166II GTNNAC 6 cut(s) 9, 340, 667, 715, 1310, 1725
Hpy8I GTNNAC 6 cut(s) 9, 340, 667, 715, 1310, 1725
Hpy99I CGWCG 1 cut(s) 116
HpyAV CCTTC 7 cut(s) 163, 414, 492, 1023, 1344, 1748, 2222
HpyCH4III ACNGT 9 cut(s) 75, 84, 832, 875, 1301, 1591, 1926, 2103, 2123
HpyCH4IV ACGT 6 cut(s) 114, 200, 550, 1576, 1684, 2422
HpyF10VI GCNNNNNNNGC 5 cut(s) 611, 1615, 1624, 1906, 2412
HpyF3I CTNAG 6 cut(s) 221, 295, 760, 1424, 2045, 2408
HpySE526I ACGT 6 cut(s) 114, 200, 550, 1576, 1684, 2422
Hsp92I GRCGYC 1 cut(s) 550
Kpn2I TCCGGA 1 cut(s) 2215
KroI GCCGGC 1 cut(s) 614
KroNI GCCGGC 1 cut(s) 616
Kzo9I GATC 8 cut(s) 276, 400, 1002, 1063, 1144, 1778, 2109, 2307
LguI GCTCTTC 1 cut(s) 1229
LmnI GCTCC 7 cut(s) 13, 67, 331, 599, 1353, 1575, 1842
Lsp1109I GCAGC 4 cut(s) 494, 574, 589, 2180
LweI GCATC 3 cut(s) 199, 1429, 1605
MabI ACCWGGT 1 cut(s) 1959
MaeI CTAG 5 cut(s) 519, 546, 887, 996, 1157
MaeII ACGT 6 cut(s) 114, 200, 550, 1576, 1684, 2422
MaeIII GTNAC 4 cut(s) 502, 1291, 1484, 1585
MalI GATC 8 cut(s) 278, 402, 1004, 1065, 1146, 1780, 2111, 2309
MboI GATC 8 cut(s) 276, 400, 1002, 1063, 1144, 1778, 2109, 2307
MfeI CAATTG 2 cut(s) 1076, 1443
MflI RGATCY 2 cut(s) 276, 1778
MhlI GDGCHC 2 cut(s) 342, 1224
MlyI GAGTC 3 cut(s) 201, 908, 2242
MmeI TCCRAC 6 cut(s) 703, 748, 775, 1597, 1864, 2134
Mph1103I ATGCAT 2 cut(s) 1087, 1620
MroI TCCGGA 1 cut(s) 2215
MroNI GCCGGC 1 cut(s) 614
MroXI GAANNNNTTC 4 cut(s) 730, 945, 1466, 1650
MseI TTAA 8 cut(s) 594, 962, 1892, 1947, 2040, 2115, 2187, 2244
MslI CAYNNNNRTG 1 cut(s) 1496
MspA1I CMGCKG 1 cut(s) 685
MspI CCGG 6 cut(s) 38, 426, 615, 638, 1115, 2216
MspR9I CCNGG 7 cut(s) 39, 266, 422, 1116, 1961, 2014, 2432
MunI CAATTG 2 cut(s) 1076, 1443
Mva1269I GAATGC 2 cut(s) 43, 1087
MvaI CCWGG 5 cut(s) 266, 422, 1961, 2014, 2432
MvnI CGCG 2 cut(s) 173, 308
MwoI GCNNNNNNNGC 5 cut(s) 611, 1615, 1624, 1906, 2412
NaeI GCCGGC 1 cut(s) 616
NciI CCSGG 2 cut(s) 39, 1116
NdeII GATC 8 cut(s) 276, 400, 1002, 1063, 1144, 1778, 2109, 2307
NgoMIV GCCGGC 1 cut(s) 614
NlaIV GGNNCC 2 cut(s) 15, 69
NmeAIII GCCGAG 1 cut(s) 238
NsiI ATGCAT 2 cut(s) 1087, 1620
NspI RCATGY 1 cut(s) 1238
NspV TTCGAA 1 cut(s) 1933
PaeI GCATGC 1 cut(s) 1238
PagI TCATGA 2 cut(s) 1066, 1611
PceI AGGCCT 1 cut(s) 2406
PciSI GCTCTTC 1 cut(s) 1229
PctI GAATGC 2 cut(s) 43, 1087
PdiI GCCGGC 1 cut(s) 616
PdmI GAANNNNTTC 4 cut(s) 730, 945, 1466, 1650
PfeI GAWTC 9 cut(s) 311, 731, 1103, 1661, 1733, 1805, 1914, 2140, 2236
PfoI TCCNGGA 3 cut(s) 264, 1114, 2430
PkrI GCNGC 6 cut(s) 175, 484, 589, 604, 2019, 2195
PleI GAGTC 3 cut(s) 200, 908, 2242
PpsI GAGTC 3 cut(s) 200, 908, 2242
PpuMI RGGWCCY 2 cut(s) 150, 2209
PsiI TTATAA 2 cut(s) 1133, 2330
Psp124BI GAGCTC 1 cut(s) 1224
Psp1406I AACGTT 2 cut(s) 1576, 2422
Psp5II RGGWCCY 2 cut(s) 150, 2209
Psp6I CCWGG 5 cut(s) 264, 420, 1959, 2012, 2430
PspGI CCWGG 5 cut(s) 264, 420, 1959, 2012, 2430
PspN4I GGNNCC 2 cut(s) 15, 69
PspPI GGNCC 6 cut(s) 150, 160, 1072, 1112, 2209, 2447
PspPPI RGGWCCY 2 cut(s) 150, 2209
PsuI RGATCY 2 cut(s) 276, 1778
PvuII CAGCTG 1 cut(s) 685
RseI CAYNNNNRTG 1 cut(s) 1496
SacI GAGCTC 1 cut(s) 1224
SapI GCTCTTC 1 cut(s) 1229
SaqAI TTAA 8 cut(s) 594, 962, 1892, 1947, 2040, 2115, 2187, 2244
SatI GCNGC 6 cut(s) 174, 483, 588, 603, 2018, 2194
Sau3AI GATC 8 cut(s) 276, 400, 1002, 1063, 1144, 1778, 2109, 2307
Sau96I GGNCC 6 cut(s) 150, 160, 1072, 1112, 2209, 2447
SchI GAGTC 3 cut(s) 201, 908, 2242
ScrFI CCNGG 7 cut(s) 39, 266, 422, 1116, 1961, 2014, 2432
SduI GDGCHC 2 cut(s) 342, 1224
SexAI ACCWGGT 1 cut(s) 1959
SfaNI GCATC 3 cut(s) 199, 1429, 1605
SfcI CTRYAG 3 cut(s) 1922, 2099, 2364
SfiI GGCCNNNNNGGCC 1 cut(s) 2412
SfuI TTCGAA 1 cut(s) 1933
SinI GGWCC 6 cut(s) 150, 160, 1072, 1112, 2209, 2447
SmiMI CAYNNNNRTG 1 cut(s) 1496
SmlI CTYRAG 5 cut(s) 121, 326, 1279, 1526, 2204
SmoI CTYRAG 5 cut(s) 121, 326, 1279, 1526, 2204
SphI GCATGC 1 cut(s) 1238
SseBI AGGCCT 1 cut(s) 2406
SsiI CCGC 7 cut(s) 173, 188, 256, 308, 1672, 1978, 2017
SspI AATATT 1 cut(s) 387
SspMI CTAG 5 cut(s) 519, 546, 887, 996, 1157
SstI GAGCTC 1 cut(s) 1224
StuI AGGCCT 1 cut(s) 2406
StyD4I CCNGG 7 cut(s) 37, 264, 420, 1114, 1959, 2012, 2430
TaaI ACNGT 9 cut(s) 75, 84, 832, 875, 1301, 1591, 1926, 2103, 2123
TaiI ACGT 6 cut(s) 117, 203, 553, 1579, 1687, 2425
TaqI TCGA 8 cut(s) 621, 1147, 1272, 1630, 1933, 1971, 2270, 2398
TatI WGTACW 4 cut(s) 84, 526, 1799, 1852
TauI GCSGC 2 cut(s) 176, 2020
TfiI GAWTC 9 cut(s) 311, 731, 1103, 1661, 1733, 1805, 1914, 2140, 2236
Tru1I TTAA 8 cut(s) 594, 962, 1892, 1947, 2040, 2115, 2187, 2244
Tru9I TTAA 8 cut(s) 594, 962, 1892, 1947, 2040, 2115, 2187, 2244
TscAI CASTG 6 cut(s) 69, 87, 1383, 1567, 1867, 2150
TseI GCWGC 4 cut(s) 482, 587, 602, 2193
TspGWI ACGGA 2 cut(s) 26, 173
TspRI CASTG 6 cut(s) 69, 87, 1383, 1567, 1867, 2150
VneI GTGCAC 1 cut(s) 338
VpaK11BI GGWCC 6 cut(s) 150, 160, 1072, 1112, 2209, 2447
XagI CCTNNNNNAGG 1 cut(s) 2216
XapI RAATTY 9 cut(s) 607, 703, 798, 854, 1326, 1395, 1410, 1768, 2281
XbaI TCTAGA 1 cut(s) 995
XceI RCATGY 1 cut(s) 1238
XcmI CCANNNNNNNNNTGG 1 cut(s) 2142
XmiI GTMKAC 1 cut(s) 714
XmnI GAANNNNTTC 4 cut(s) 730, 945, 1466, 1650
XspI CTAG 5 cut(s) 519, 546, 887, 996, 1157
ZraI GACGTC 1 cut(s) 551
Zsp2I ATGCAT 2 cut(s) 1087, 1620
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.