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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccI | GTMKAC | 1 cut(s) | 5 |
| AccIII | TCCGGA | 1 cut(s) | 157 |
| AciI | CCGC | 2 cut(s) | 250, 321 |
| AclWI | GGATC | 2 cut(s) | 260, 419 |
| AcoI | YGGCCR | 1 cut(s) | 318 |
| AcsI | RAATTY | 1 cut(s) | 293 |
| AgsI | TTSAA | 2 cut(s) | 279, 298 |
| AjnI | CCWGG | 1 cut(s) | 55 |
| AluBI | AGCT | 2 cut(s) | 44, 192 |
| AluI | AGCT | 2 cut(s) | 44, 192 |
| Alw26I | GTCTC | 2 cut(s) | 149, 168 |
| AlwI | GGATC | 2 cut(s) | 260, 419 |
| Ama87I | CYCGRG | 1 cut(s) | 84 |
| Aor13HI | TCCGGA | 1 cut(s) | 157 |
| AoxI | GGCC | 2 cut(s) | 58, 318 |
| ApeKI | GCWGC | 1 cut(s) | 189 |
| ApoI | RAATTY | 1 cut(s) | 293 |
| AvaI | CYCGRG | 1 cut(s) | 84 |
| BbsI | GAAGAC | 1 cut(s) | 321 |
| BbvI | GCAGC | 1 cut(s) | 201 |
| BccI | CCATC | 1 cut(s) | 312 |
| BceAI | ACGGC | 1 cut(s) | 45 |
| BciT130I | CCWGG | 1 cut(s) | 57 |
| BclI | TGATCA | 1 cut(s) | 226 |
| BcoDI | GTCTC | 2 cut(s) | 149, 168 |
| BfmI | CTRYAG | 1 cut(s) | 187 |
| BisI | GCNGC | 2 cut(s) | 190, 321 |
| BlsI | GCNGC | 2 cut(s) | 191, 322 |
| Bme1390I | CCNGG | 1 cut(s) | 57 |
| BmeT110I | CYCGRG | 1 cut(s) | 84 |
| BmrFI | CCNGG | 1 cut(s) | 57 |
| BpiI | GAAGAC | 1 cut(s) | 321 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 78, 110 |
| BpmI | CTGGAG | 2 cut(s) | 80, 120 |
| BpuEI | CTTGAG | 1 cut(s) | 30 |
| BsaWI | WCCGGW | 1 cut(s) | 157 |
| Bse1I | ACTGG | 4 cut(s) | 97, 137, 386, 402 |
| Bse3DI | GCAATG | 1 cut(s) | 295 |
| BseAI | TCCGGA | 1 cut(s) | 157 |
| BseBI | CCWGG | 1 cut(s) | 57 |
| BseMI | GCAATG | 1 cut(s) | 295 |
| BseMII | CTCAG | 1 cut(s) | 235 |
| BseNI | ACTGG | 4 cut(s) | 97, 137, 386, 402 |
| BseRI | GAGGAG | 2 cut(s) | 156, 159 |
| BseXI | GCAGC | 1 cut(s) | 201 |
| BseYI | CCCAGC | 1 cut(s) | 323 |
| BshFI | GGCC | 2 cut(s) | 60, 320 |
| BsiHKCI | CYCGRG | 1 cut(s) | 84 |
| BsiSI | CCGG | 1 cut(s) | 158 |
| BsmAI | GTCTC | 2 cut(s) | 149, 168 |
| BsmBI | CGTCTC | 1 cut(s) | 149 |
| BsnI | GGCC | 2 cut(s) | 60, 320 |
| BsoBI | CYCGRG | 1 cut(s) | 84 |
| Bsp13I | TCCGGA | 1 cut(s) | 157 |
| Bsp143I | GATC | 3 cut(s) | 226, 252, 411 |
| BspACI | CCGC | 2 cut(s) | 250, 321 |
| BspANI | GGCC | 2 cut(s) | 60, 320 |
| BspCNI | CTCAG | 1 cut(s) | 234 |
| BspEI | TCCGGA | 1 cut(s) | 157 |
| BspMAI | CTGCAG | 1 cut(s) | 191 |
| BspPI | GGATC | 2 cut(s) | 260, 419 |
| BsrDI | GCAATG | 1 cut(s) | 295 |
| BsrI | ACTGG | 4 cut(s) | 97, 137, 386, 402 |
| BssMI | GATC | 3 cut(s) | 226, 252, 411 |
| BssNAI | GTATAC | 1 cut(s) | 6 |
| Bst1107I | GTATAC | 1 cut(s) | 6 |
| Bst2UI | CCWGG | 1 cut(s) | 57 |
| Bst4CI | ACNGT | 1 cut(s) | 389 |
| Bst6I | CTCTTC | 1 cut(s) | 280 |
| BstDEI | CTNAG | 1 cut(s) | 221 |
| BstKTI | GATC | 3 cut(s) | 229, 255, 414 |
| BstMAI | GTCTC | 2 cut(s) | 149, 168 |
| BstMBI | GATC | 3 cut(s) | 226, 252, 411 |
| BstNI | CCWGG | 1 cut(s) | 57 |
| BstSCI | CCNGG | 1 cut(s) | 55 |
| BstSFI | CTRYAG | 1 cut(s) | 187 |
| BstV1I | GCAGC | 1 cut(s) | 201 |
| BstV2I | GAAGAC | 1 cut(s) | 321 |
| BstX2I | RGATCY | 1 cut(s) | 252 |
| BstYI | RGATCY | 1 cut(s) | 252 |
| BstZ17I | GTATAC | 1 cut(s) | 6 |
| BsuRI | GGCC | 2 cut(s) | 60, 320 |
| BtsIMutI | CAGTG | 2 cut(s) | 229, 395 |
| CviJI | RGCY | 6 cut(s) | 44, 50, 60, 192, 207, 320 |
| CviKI_1 | RGCY | 6 cut(s) | 44, 50, 60, 192, 207, 320 |
| DdeI | CTNAG | 1 cut(s) | 221 |
| DpnI | GATC | 3 cut(s) | 228, 254, 413 |
| DpnII | GATC | 3 cut(s) | 226, 252, 411 |
| EaeI | YGGCCR | 1 cut(s) | 318 |
| Eam1104I | CTCTTC | 1 cut(s) | 280 |
| EarI | CTCTTC | 1 cut(s) | 280 |
| EciI | GGCGGA | 1 cut(s) | 265 |
| Eco88I | CYCGRG | 1 cut(s) | 84 |
| EcoRI | GAATTC | 1 cut(s) | 293 |
| EcoRII | CCWGG | 1 cut(s) | 55 |
| Esp3I | CGTCTC | 1 cut(s) | 149 |
| FaiI | YATR | 1 cut(s) | 6 |
| FbaI | TGATCA | 1 cut(s) | 226 |
| FblI | GTMKAC | 1 cut(s) | 5 |
| Fnu4HI | GCNGC | 2 cut(s) | 190, 321 |
| Fsp4HI | GCNGC | 2 cut(s) | 190, 321 |
| GluI | GCNGC | 2 cut(s) | 190, 321 |
| GsaI | CCCAGC | 1 cut(s) | 327 |
| GsuI | CTGGAG | 2 cut(s) | 80, 120 |
| HaeIII | GGCC | 2 cut(s) | 60, 320 |
| HapII | CCGG | 1 cut(s) | 158 |
| HinfI | GANTC | 4 cut(s) | 87, 93, 131, 161 |
| HpaII | CCGG | 1 cut(s) | 158 |
| Hpy166II | GTNNAC | 2 cut(s) | 6, 102 |
| Hpy188I | TCNGA | 2 cut(s) | 92, 309 |
| Hpy188III | TCNNGA | 4 cut(s) | 84, 128, 158, 230 |
| Hpy8I | GTNNAC | 2 cut(s) | 6, 102 |
| HpyCH4III | ACNGT | 1 cut(s) | 389 |
| HpyCH4V | TGCA | 3 cut(s) | 189, 348, 361 |
| HpyF3I | CTNAG | 1 cut(s) | 221 |
| Kpn2I | TCCGGA | 1 cut(s) | 157 |
| Ksp22I | TGATCA | 1 cut(s) | 226 |
| Kzo9I | GATC | 3 cut(s) | 226, 252, 411 |
| Lsp1109I | GCAGC | 1 cut(s) | 201 |
| MalI | GATC | 3 cut(s) | 228, 254, 413 |
| MboI | GATC | 3 cut(s) | 226, 252, 411 |
| MboII | GAAGA | 2 cut(s) | 267, 321 |
| MfeI | CAATTG | 1 cut(s) | 362 |
| MflI | RGATCY | 1 cut(s) | 252 |
| MluCI | AATT | 5 cut(s) | 67, 279, 293, 349, 362 |
| MlyI | GAGTC | 3 cut(s) | 87, 96, 170 |
| MmeI | TCCRAC | 1 cut(s) | 115 |
| MnlI | CCTC | 3 cut(s) | 177, 180, 283 |
| MroI | TCCGGA | 1 cut(s) | 157 |
| MseI | TTAA | 1 cut(s) | 74 |
| MspA1I | CMGCKG | 1 cut(s) | 323 |
| MspI | CCGG | 1 cut(s) | 158 |
| MspR9I | CCNGG | 1 cut(s) | 57 |
| MunI | CAATTG | 1 cut(s) | 362 |
| MvaI | CCWGG | 1 cut(s) | 57 |
| NdeII | GATC | 3 cut(s) | 226, 252, 411 |
| NmeAIII | GCCGAG | 1 cut(s) | 183 |
| PfeI | GAWTC | 1 cut(s) | 131 |
| PkrI | GCNGC | 2 cut(s) | 191, 322 |
| PleI | GAGTC | 3 cut(s) | 87, 95, 169 |
| PpsI | GAGTC | 3 cut(s) | 87, 95, 169 |
| Psp6I | CCWGG | 1 cut(s) | 55 |
| PspFI | CCCAGC | 1 cut(s) | 323 |
| PspGI | CCWGG | 1 cut(s) | 55 |
| PstI | CTGCAG | 1 cut(s) | 191 |
| PsuI | RGATCY | 1 cut(s) | 252 |
| SaqAI | TTAA | 1 cut(s) | 74 |
| SatI | GCNGC | 2 cut(s) | 190, 321 |
| Sau3AI | GATC | 3 cut(s) | 226, 252, 411 |
| SchI | GAGTC | 3 cut(s) | 87, 96, 170 |
| ScrFI | CCNGG | 1 cut(s) | 57 |
| SetI | ASST | 2 cut(s) | 46, 194 |
| SfcI | CTRYAG | 1 cut(s) | 187 |
| SmlI | CTYRAG | 1 cut(s) | 45 |
| SmoI | CTYRAG | 1 cut(s) | 45 |
| Sse9I | AATT | 5 cut(s) | 67, 279, 293, 349, 362 |
| SsiI | CCGC | 2 cut(s) | 250, 321 |
| StyD4I | CCNGG | 1 cut(s) | 55 |
| TaaI | ACNGT | 1 cut(s) | 389 |
| TaqI | TCGA | 3 cut(s) | 115, 121, 129 |
| TasI | AATT | 5 cut(s) | 67, 279, 293, 349, 362 |
| TauI | GCSGC | 1 cut(s) | 323 |
| TfiI | GAWTC | 1 cut(s) | 131 |
| Tru1I | TTAA | 1 cut(s) | 74 |
| Tru9I | TTAA | 1 cut(s) | 74 |
| TscAI | CASTG | 2 cut(s) | 229, 402 |
| TseI | GCWGC | 1 cut(s) | 189 |
| TspDTI | ATGAA | 1 cut(s) | 306 |
| TspRI | CASTG | 2 cut(s) | 229, 402 |
| XapI | RAATTY | 1 cut(s) | 293 |
| XmiI | GTMKAC | 1 cut(s) | 5 |