MD12G1248800.v1.1

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0000055
Physical Location & Seq
Reverse (-)
31848522 .. 31853848
5327 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1248800.v1.1.491

Sequence Viewer

Length: 3549 bp
ATGGTGCTGGTGTTACTGTCCTACCACTATGCTCATCGCAATTCCGGTCTTGTCTTTGATGTCCTCATCTTCCTCCTCTGCTCCCTCGGCTTCGTCTTTCGCCAAATGAATATCTCTGTCCCCGATAAAACCTCCTCCTCCTCCTCCTCCTCACCCTCATCCTCATCGTCCTCCTCACCCTCATCCTCATCCTCCTCGTCCTCCTCGTCCTCCTCCACCTCCTCCACCTCCTCACCCTCAATCTCATCTTCATCGTCCACCTCTGCCTCCCCGTCCCTGTCCTCCTTCTCAAAAGGCTTGCTCTACGAAGTGTTCATAAGCTTCAGAGGCGAAGACACACGTAAAAACTTCACGGGCCACCTCCACGAAGCATTGACAAAGGCCGGAATCAACGCCTTTATTGACGACGAACTAAGAAGAGGAGAAGATATAACTACCGAACTTGTGCAGGCAATCCAGGGTTCTAGGATCTCTATCATCGTCTTCTCAAGACGGTACTCAGACTCCAGCTGGTGTCTCGAGGAGCTGGTTAAGGTCATGGAGTGTAGAAGAACGCTAGGGCAATTAGTTTTGCCGATATTCTATGACGTTGATCCTTCGCATGTCAGGAAACAGACCGGTAGATTTGCACAATCGTTTCTGAAACATACAGATGAAAAAAAGGTAGAGAGGTGGAGAGCTGCTCTTACTGAAGCTTCGAATTTGTCTGGCTGGGATCTCAGAAACACTTTGGACGGGCATGAAGCAAAGTTTATCAGGATGATTACCAATGACGTCACTACGAAGTTGAACAACAAATACTTTGACGTAGCGCCCTATCAAGTCGGAATAGATACTCGAGTGCTAGATATCAGTAATTATTTAGGCATCGGAGATTCAGATGATGTTCGTGTGATTGGAATTTCAGGCATGGGTGGAATAGGTAAAACAACGATTGCTCAAGCCATTTATAACATATTTTATGAAAGGTTTGAAGGTAAAAGTTTCCTTGAAAAAGTGAGGGAAAAGAAACTAGAAAAATTGCAAAAACAACTTCTTTTCGATATCTTGCAAACCAAGACAAAGGTAAGCAGTGTTGTTGCAGGGACCGCCTTGGTAAGGGAAAGATTTCGACGCTTAAAGGTACTTGTCATAGTTGATGATGTAGACGATGTGAAGCAGTTACGCGAATTAGTTGGAAATTGCCACTTTTTTGGCCCGGGGAGCAGAATCATCATCACAACTAGAAACGAACGTGTGCTAAAAGAATTTGCAGTTGATAAGATATATCGGGCGAAAGTAATGGACCGAGAAGAAGCTCTTGAGCTCCTAAGTTGGCATGCTTTCAGAAGTAGTAGTTGTCCTAGTCAATATCTTGCGCTTGAAAGAGAAGTTGTCAATTACTGTGGAGGATTGCCGCTGGCTCTTGAAGTTTTAGGATCTACTCTTTTCAAACGAAGCGTAGATGAATGGAGAAGTATATTGGATGAATTGAAAATGATTCCTCGTGGAGAAATTCAGGCACAACTGAAAATAAGCTACGACGGGCTAAATGATAATTACAAGAGGCGGATATTCCTCGATATAGCTTGTTTTTTTATCGGAATGGACAAGAACGATGTCGTGCAAATCTTGGATGGTTGTGGCTTTTATTCAACAACAGGAATCGAGGTCCTCCTTAACCGGTGCCTAGTGACTATTAATAGAGAAAACAAGATTATGATGCATGATTTGCTTCGAGATATGGGCAGAGATATCGTGCATGCAGAAAATCCCGATTTTCCTGGAGAACGGAGTAGATTGTGGCATCCCGAAGATGTAAATGATGTATTGATAGACAAATCTGGAACTGAAAAAATTGAAGGTCTAGCTTTGAATTTGCCGAGTCTTGAAGAGACTAGTTTCAGTACTGAGGTGTTTAGAAATATGAAGAGACTGAGATTGCTCCAACTAAACTACGTTCGGCTCTCTGGGGGATACCAATGTCTTTCCAAAAAATTAAAATGGCTGTGCTGGCATGGATTCCCATTGGAGTTCATACCAATAGAACTGTGTCAACCAAACATAGTTGTTATCGACATGCAGTACAGCAACCTCAGACAAGTTCTTTGTGAGTATTCTGGGTTGCTTGATAAGTTGAAGATTCTAAATCTCAGCCACTCCCACGATCTAACACAATCGCCAGACTTTTCAAAATTCCCAAATCTTGAGAAATTGATACTCAAAGACTGTAAGAGGTTGGCTAAAGTTCACAAGTCCATCGGAGATCTTAAGAGTCTTGTGTTGGTGAATTTGAAAGACTGTGAAACGCTTAAGGCTCTTCCGAGGAGTTTCTACAAGTTGAAATCTGTTAAAACTCTTGTTCTCGATGGTTGTTCAAGATTCCAAAGTTTGGCTGAGCACTTGGGAGAAATGGCATCATTGGTCACTCTTTATGCAGATGGAACGGCCATAAAAAAACTACCACCTTCCATCGTACGACTAGAGAAGCTCGAGCGCTTATCTTTGAGTAAATTGAAGTGTTCTTTGCAGCTACCTTCCTTACTAGGTTTACGCTCTTTGACAAGTGTAATTTTGGAGGACAGCAATATAGAGGAAGTGCCTAATGATATTGGGAGTAGTCTACCTTGTTTAGTGGAGTTAAGTCTAGATGACAATAATTTTGGGAGCCTTCCAAGCCTCAGTGGCCTCTCCAAGCTTCGTATACTATCCTTAAATGGTTGCAGAAACCTTGTCGAGATTACAGATTTACCAAAAAGTTTGGGTTTCCTGCAAATGGATGACTGCTCTGCATTAGAAAGAATGCCAGATTTTTCAGGCATGTCAACAATGGTGAGTCTCTTTTCCCCGAAACTCATTGAGTTTCCAGGCTTGGATAGCGCGTTAAACTCGGGACTCCATCTTTGTATGATAACACACAACAATGTCATGGATTTCCTTCTTAAGGATAGCACGATACAGGGATGGACAGGAGGTGGAACCATGAGTTTTGTAGGAAGACAAATTCCCACTTGGTTCAATCATGTCAACGAGGGTACCCAAGTCTCTTTTGAAGTGCCCAAGGAAATTGGATGTAATGCAAAAGCGTTGGCTGTGTGCCTGGCTTTTGTTCCTCGTGATTACATCAACGAGGGTACTAGTTTTTGTGTCGAACTAGGAGATCTTTTTCTCATTGAAGAGATGATTTGGCTGGCAAATTTATCGTTGTCGGAAACTGAGTTCAATTTGGGAGAAAGCGATTTGGTCCATGTTATTGCACATTGTCCGGTGAAGAAAATAGGGGTACGTTTAGTATGCGACAAACTTATGACTTTCAAAGGTTCATTATTTGATTACCATTATATCCCTTACGAACGGGCCTTAGAAGAAATTTCTACCAAAGGTGATGATTTTGATGAGGATTATGAAGATGATGATTTTGATGAGGATGATGACAACACGATGAGGATGATGAGAAGGATTTTAATGAGGAGGATGAGGAATAAGACGATGATCAGGACCAAGAAGTTGCGGATGATGATGATGAGGGTGATGATGAGGAGGAGGACCAAGACGACGACGACTATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

1183

Amino Acids

133.55

Weight (kDa)

6.67

Isoelectric Point (pI)

47.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 102 - 266 1e-52 TIR domain
TIR_2 PF13676 104 - 200 2.1e-16 TIR domain
NB-ARC PF00931 293 - 445 2e-19 NB-ARC domain
WHD_ROQ1 PF23282 515 - 581 1.7e-18 Disease resistance protein Roq1-like, winged-helix domain
LRR_8 PF13855 845 - 902 1.8e-06 Leucine rich repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000055)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G36930 AT5G36930 AT5G36930 AT5G36930
fragaria_vesca FvH4_3g45361 FvH4_3g45380 FvH4_5g21061 FvH4_5g21062 FvH4_5g21063 FvH4_5g32050 FvH4_5g32970 FvH4_5g32990 FvH4_5g34190 FvH4_5g34210 FvH4_5g34210 FvH4_5g34210 FvH4_5g34210 FvH4_5g34210 FvH4_5g34210 FvH4_5g34211 FvH4_5g34240 FvH4_5g34250
malus_domestica MD00G1011700.v1.1 MD00G1026100.v1.1 MD00G1026500.v1.1 MD00G1027000.v1.1 MD00G1027100.v1.1 MD00G1027700.v1.1 MD00G1027800.v1.1 MD00G1028000.v1.1 MD00G1220000.v1.1 MD02G1024400.v1.1 MD02G1024800.v1.1 MD02G1026000.v1.1 MD02G1026100.v1.1 MD02G1031800.v1.1 MD02G1038500.v1.1 MD02G1038700.v1.1 MD02G1039200.v1.1 MD02G1040600.v1.1 MD02G1040900.v1.1 MD02G1041200.v1.1 MD02G1041300.v1.1 MD02G1041700.v1.1 MD02G1042000.v1.1 MD02G1043800.v1.1 MD02G1043900.v1.1 MD02G1044300.v1.1 MD02G1045200.v1.1 MD02G1045300.v1.1 MD02G1051200.v1.1 MD02G1052200.v1.1 MD02G1052800.v1.1 MD02G1053100.v1.1 MD02G1053600.v1.1 MD02G1055500.v1.1 MD02G1063300.v1.1 MD02G1063400.v1.1 MD02G1063800.v1.1 MD02G1306500.v1.1 MD02G1306600.v1.1 MD02G1306700.v1.1 MD02G1306900.v1.1 MD02G1307200.v1.1 MD04G1011700.v1.1 MD05G1315400.v1.1 MD05G1316000.v1.1 MD05G1316700.v1.1 MD05G1317000.v1.1 MD05G1317300.v1.1 MD05G1317600.v1.1 MD05G1317700.v1.1 MD07G1102800.v1.1 MD07G1238200.v1.1 MD08G1175900.v1.1 MD08G1203600.v1.1 MD09G1024200.v1.1 MD09G1039600.v1.1 MD09G1044800.v1.1 MD09G1045100.v1.1 MD10G1038700.v1.1 MD10G1039000.v1.1 MD10G1039200.v1.1 MD10G1040000.v1.1 MD10G1040200.v1.1 MD10G1040600.v1.1 MD10G1040800.v1.1 MD10G1297300.v1.1 MD12G1247500.v1.1 MD12G1247900.v1.1 MD12G1248300.v1.1 MD12G1248800.v1.1 MD15G1103700.v1.1 MD15G1103800.v1.1 MD15G1104000.v1.1 MD15G1179600.v1.1 MD15G1182500.v1.1 MD16G1068200.v1.1 MD16G1068300.v1.1 MD17G1024600.v1.1 MD17G1024700.v1.1 MD17G1025000.v1.1 MD17G1025900.v1.1
prunus_persica Prupe.1G539800_v2.0.a1 Prupe.1G550800_v2.0.a1 Prupe.1G550800_v2.0.a1 Prupe.1G550800_v2.0.a1 Prupe.1G550800_v2.0.a1 Prupe.1G550800_v2.0.a1 Prupe.1G550800_v2.0.a1 Prupe.1G557100_v2.0.a1 Prupe.1G557200_v2.0.a1 Prupe.1G557200_v2.0.a1 Prupe.1G557300_v2.0.a1
pyrus_communis pycom02g02080 pycom02g02090 pycom02g02110 pycom02g03220 pycom02g03260 pycom02g03370 pycom02g03390 pycom02g03410 pycom02g03420 pycom02g03450 pycom02g03500 pycom02g03730 pycom02g03770 pycom02g04400 pycom02g04410 pycom02g04430 pycom02g04450 pycom02g04460 pycom02g04490 pycom02g05200 pycom02g25710 pycom02g25750 pycom02g25790 pycom02g25810 pycom02g25830 pycom04g00920 pycom04g00930 pycom04g00940 pycom05g29300 pycom05g29310 pycom05g29380 pycom05g29410 pycom07g06310 pycom07g21420 pycom08g15070 pycom08g17540 pycom08g17560 pycom08g17570 pycom10g02670 pycom10g02680 pycom10g02700 pycom10g02770 pycom10g02790 pycom10g02800 pycom10g02810 pycom10g25020 pycom10g25030 pycom10g25050 pycom10g25080 pycom111g01990 pycom111g02000 pycom111g02030 pycom111g02040 pycom111g02050 pycom111g02060 pycom12g22710 pycom12g22720 pycom12g22730 pycom12g22740 pycom12g22750 pycom12g22770 pycom12g22780 pycom12g22790 pycom12g22800 pycom12g22810 pycom12g22830 pycom12g22840 pycom12g22860 pycom15g09440 pycom15g09480 pycom15g16120 pycom15g32380 pycom16g05970 pycom17g02100
rosa_chinensis RchiOBHm_Chr2g0144661 RchiOBHm_Chr4g0407491 RchiOBHm_Chr4g0407501 RchiOBHm_Chr4g0412381 RchiOBHm_Chr7g0208191 RchiOBHm_Chr7g0208201 RchiOBHm_Chr7g0208211 RchiOBHm_Chr7g0208221 RchiOBHm_Chr7g0228071 RchiOBHm_Chr7g0228121 RchiOBHm_Chr7g0230971 RchiOBHm_Chr7g0230981 RchiOBHm_Chr7g0233901 RchiOBHm_Chr7g0233951 RchiOBHm_Chr7g0233961 RchiOBHm_Chr7g0233991 RchiOBHm_Chr7g0234021 RchiOBHm_Chr7g0234151 RchiOBHm_Chr7g0234161 RchiOBHm_Chr7g0234241 RchiOBHm_Chr7g0234501 RchiOBHm_Chr7g0234641 RchiOBHm_Chr7g0234771 RchiOBHm_Chr7g0234781 RchiOBHm_Chr7g0234991 RchiOBHm_Chr7g0241291 RchiOBHm_Chr7g0241301
rosa_laevigata RLG00000001186 RLG00000001187 RLG00000001212 RLG00000001226 RLG00000001233 RLG00000001590 RLG00000003242 RLG00000003243 RLG00000003249 RLG00000008688 RLG00000008690
rosa_multiflora Rmu_co7966574.1_g000001 Rmu_co8120830.1_g000001 Rmu_co8142226.1_g000001 Rmu_co8293083.1_g000001 Rmu_co8351227.1_g000001 Rmu_co8380159.1_g000001 Rmu_co8456505.1_g000001 Rmu_sc0000676.1_g000025 Rmu_sc0000706.1_g000018 Rmu_sc0000706.1_g000040 Rmu_sc0000740.1_g000029 Rmu_sc0000740.1_g000032 Rmu_sc0000740.1_g000037 Rmu_sc0000740.1_g000040 Rmu_sc0000762.1_g000003 Rmu_sc0000762.1_g000019 Rmu_sc0000762.1_g000034 Rmu_sc0000762.1_g000069 Rmu_sc0000892.1_g000001 Rmu_sc0000945.1_g000040 Rmu_sc0001913.1_g000037 Rmu_sc0001913.1_g000041 Rmu_sc0001913.1_g000049 Rmu_sc0002775.1_g000017 Rmu_sc0002775.1_g000018 Rmu_sc0002775.1_g000020 Rmu_sc0002775.1_g000021 Rmu_sc0002775.1_g000022 Rmu_sc0002775.1_g000025 Rmu_sc0002775.1_g000026 Rmu_sc0002775.1_g000028 Rmu_sc0002775.1_g000030 Rmu_sc0004858.1_g000002 Rmu_sc0004864.1_g000022 Rmu_sc0005038.1_g000023 Rmu_sc0005840.1_g000014 Rmu_sc0006724.1_g000003 Rmu_sc0006824.1_g000005 Rmu_sc0007869.1_g000003 Rmu_sc0007869.1_g000005 Rmu_sc0007869.1_g000008 Rmu_sc0008646.1_g000007 Rmu_sc0009637.1_g000009 Rmu_sc0010500.1_g000002 Rmu_sc0010500.1_g000012 Rmu_sc0012403.1_g000025 Rmu_sc0013768.1_g000025 Rmu_sc0015625.1_g000001 Rmu_sc0019715.1_g000001 Rmu_sc0021133.1_g000002 Rmu_sc0021904.1_g000001 Rmu_sc0021904.1_g000003 Rmu_sc0021904.1_g000004 Rmu_sc0022054.1_g000001 Rmu_sc0026025.1_g000001 Rmu_sc0036828.1_g000002 Rmu_sc0041808.1_g000001 Rmu_ssc0000012.1_g000001
rosa_roxburghii Rroxscaffold_3G00226370 Rroxscaffold_3G00226860 Rroxscaffold_3G00226900 Rroxscaffold_3G00226930 Rroxscaffold_3G00226940 Rroxscaffold_3G00226970 Rroxscaffold_3G00226980 Rroxscaffold_3G00231530 Rroxscaffold_3G00237090 Rroxscaffold_3G00250760 Rroxscaffold_3G00250800
rosa_rugosa Rorug04G0082300 Rorug07G0101800 Rorug07G0102000 Rorug07G0102100 Rorug07G0221200 Rorug07G0245700 Rorug07G0245700 Rorug07G0245700 Rorug07G0263900 Rorug07G0264000 Rorug07G0264000 Rorug07G0264500 Rorug07G0282900 Rorug07G0282900 Rorug07G0283100 Rorug07G0283200.1 Rorug07G0283300 Rorug07G0283900 Rorug07G0284000 Rorug07G0284100 Rorug07G0284300 Rorug07G0284400 Rorug07G0284500 Rorug07G0285000 Rorug07G0285200 Rorug07G0285300 Rorug07G0285500 Rorug07G0286800 Rorug07G0286900 Rorug07G0287100 Rorug07G0287200
rosa_samantha Rh7AG236100 Rh7AG236200 Rh7AG236300 Rh7AG440000 Rh7AG440100 Rh7BG411900 Rh7BG412000 Rh7BG412600 Rh7CG251300 Rh7CG417700 Rh7CG460100 Rh7DG242100 Rh7DG242200 Rh7DG242300 Rh7DG429300
rosa_wichuraiana Rw0G005070 Rw0G010780 Rw0G012530 Rw7G020110 Rw7G020160 Rw7G020170 Rw7G033210 Rw7G033230 Rw7G033250 Rw7G034540 Rw7G035150 Rw7G036190 Rw7G036210 Rw7G036260 Rw7G036270 Rw7G036290 Rw7G036300 Rw7G036310 Rw7G036350 Rw7G036360 Rw7G036440 Rw7G036510 Rw7G036530 Rw7G036570 Rw7G036590 Rw7G036730

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 951
AatII GACGTC 1 cut(s) 777
Acc65I GGTACC 1 cut(s) 3014
AccB1I GGYRCC 2 cut(s) 1665, 3014
AccB7I CCANNNNNTGG 1 cut(s) 2371
AccI GTMKAC 3 cut(s) 1146, 2602, 2683
AccII CGCG 2 cut(s) 1167, 2861
AciI CCGC 4 cut(s) 1089, 1397, 1549, 3490
AclWI GGATC 4 cut(s) 476, 587, 723, 1426
AcoI YGGCCR 1 cut(s) 2427
AcuI CTGAAG 2 cut(s) 307, 711
AcyI GRCGYC 1 cut(s) 774
AfaI GTAC 8 cut(s) 497, 1125, 1888, 2066, 2457, 3016, 3115, 3264
AfeI AGCGCT 1 cut(s) 2477
AfiI CCNNNNNNNGG 4 cut(s) 1098, 2371, 2755, 2923
AflII CTTAAG 3 cut(s) 2249, 2291, 2921
AflIII ACRYGT 2 cut(s) 338, 1234
AgeI ACCGGT 2 cut(s) 617, 1662
AhlI ACTAGT 2 cut(s) 1877, 3116
AjnI CCWGG 4 cut(s) 456, 1762, 2845, 3078
AloI GAACNNNNNNTCC 2 cut(s) 3182, 3214
Alw21I GWGCWC 2 cut(s) 1308, 2382
Alw26I GTCTC 5 cut(s) 521, 1868, 1906, 2822, 3028
AlwI GGATC 4 cut(s) 476, 587, 723, 1426
Ama87I CYCGRG 5 cut(s) 518, 837, 1198, 2471, 2869
Aor51HI AGCGCT 1 cut(s) 2477
AoxI GGCC 6 cut(s) 355, 381, 1195, 2427, 2665, 3336
ApeKI GCWGC 2 cut(s) 680, 2509
ArsI GACNNNNNNTTYG 6 cut(s) 2070, 2102, 2624, 2656, 2865, 2897
AseI ATTAAT 1 cut(s) 1680
AsiGI ACCGGT 2 cut(s) 617, 1662
Asp700I GAANNNNTTC 1 cut(s) 1107
Asp718I GGTACC 1 cut(s) 3014
AspLEI GCGC 4 cut(s) 814, 1360, 2478, 2861
AspS9I GGNCC 9 cut(s) 355, 1086, 1196, 1285, 1651, 3223, 3336, 3477, 3525
AsuC2I CCSGG 2 cut(s) 1199, 1200
AsuHPI GGTGA 8 cut(s) 144, 168, 225, 2278, 2824, 3259, 3374, 3520
AsuII TTCGAA 1 cut(s) 698
AvaI CYCGRG 5 cut(s) 518, 837, 1198, 2471, 2869
AvaII GGWCC 6 cut(s) 1086, 1285, 1651, 3223, 3477, 3525
BaeGI GKGCMC 1 cut(s) 3039
BaeI ACNNNNGTAYC 8 cut(s) 825, 858, 1948, 1981, 2998, 3006, 3031, 3039
BanI GGYRCC 2 cut(s) 1665, 3014
BanII GRGCYC 1 cut(s) 1308
BauI CACGAG 2 cut(s) 1485, 3093
BbsI GAAGAC 3 cut(s) 339, 475, 2983
Bbv12I GWGCWC 2 cut(s) 1308, 2382
BbvI GCAGC 2 cut(s) 667, 2521
BccI CCATC 7 cut(s) 1610, 2246, 2342, 2414, 2459, 2886, 2937
BceAI ACGGC 1 cut(s) 2442
BcgI CGANNNNNNTGC 4 cut(s) 1717, 1751, 3162, 3196
BciT130I CCWGG 4 cut(s) 458, 1764, 2847, 3080
BciVI GTATCC 1 cut(s) 1949
BclI TGATCA 1 cut(s) 3471
BcnI CCSGG 2 cut(s) 1199, 1200
BcoDI GTCTC 5 cut(s) 521, 1868, 1906, 2822, 3028
BcuI ACTAGT 2 cut(s) 1877, 3116
BfoI RGCGCY 2 cut(s) 815, 2479
BfrI CTTAAG 3 cut(s) 2249, 2291, 2921
BfuI GTATCC 1 cut(s) 1949
BglI GCCNNNNNGGC 1 cut(s) 2664
BglII AGATCT 2 cut(s) 2245, 3139
BisI GCNGC 3 cut(s) 681, 1397, 2510
BlpI GCTNAGC 1 cut(s) 2376
BlsI GCNGC 3 cut(s) 682, 1398, 2511
BmcAI AGTACT 1 cut(s) 1888
Bme1390I CCNGG 6 cut(s) 458, 1199, 1200, 1764, 2847, 3080
Bme18I GGWCC 6 cut(s) 1086, 1285, 1651, 3223, 3477, 3525
BmeT110I CYCGRG 5 cut(s) 518, 837, 1198, 2471, 2869
BmgT120I GGNCC 9 cut(s) 355, 1086, 1196, 1285, 1651, 3223, 3336, 3477, 3525
BmiI GGNNCC 5 cut(s) 1087, 1667, 2648, 2959, 3016
BmrFI CCNGG 6 cut(s) 458, 1199, 1200, 1764, 2847, 3080
BmsI GCATC 4 cut(s) 876, 1692, 1795, 2405
BpiI GAAGAC 3 cut(s) 339, 475, 2983
BplI GAGNNNNNCTC 2 cut(s) 667, 699
BpmI CTGGAG 2 cut(s) 490, 1785
Bpu1102I GCTNAGC 1 cut(s) 2376
Bpu14I TTCGAA 1 cut(s) 698
BpuEI CTTGAG 4 cut(s) 472, 924, 1322, 2207
BpuMI CCSGG 2 cut(s) 1199, 1200
BsaAI YACGTR 1 cut(s) 341
BsaBI GATNNNNATC 1 cut(s) 473
BsaHI GRCGYC 1 cut(s) 774
BsaJI CCNNGG 7 cut(s) 85, 457, 1092, 1198, 1199, 2303, 3039
BsaWI WCCGGW 4 cut(s) 44, 617, 1662, 3244
BsaXI ACNNNNNCTCC 2 cut(s) 488, 518
Bsc4I CCNNNNNNNGG 4 cut(s) 1098, 2371, 2755, 2923
Bse118I RCCGGY 2 cut(s) 617, 1662
Bse8I GATNNNNATC 1 cut(s) 473
BseBI CCWGG 4 cut(s) 458, 1764, 2847, 3080
BseDI CCNNGG 7 cut(s) 85, 457, 1092, 1198, 1199, 2303, 3039
BseJI GATNNNNATC 1 cut(s) 473
BseLI CCNNNNNNNGG 4 cut(s) 1098, 2371, 2755, 2923
BseMII CTCAG 9 cut(s) 513, 733, 1881, 1907, 2089, 2146, 2367, 2674, 3186
BseSI GKGCMC 1 cut(s) 3039
BseXI GCAGC 2 cut(s) 667, 2521
BseYI CCCAGC 1 cut(s) 711
BsgI GTGCAG 1 cut(s) 467
Bsh1236I CGCG 2 cut(s) 1167, 2861
BshFI GGCC 6 cut(s) 357, 383, 1197, 2429, 2667, 3338
BshNI GGYRCC 2 cut(s) 1665, 3014
BshTI ACCGGT 2 cut(s) 617, 1662
BsiHKAI GWGCWC 2 cut(s) 1308, 2382
BsiHKCI CYCGRG 5 cut(s) 518, 837, 1198, 2471, 2869
BsiSI CCGG 6 cut(s) 45, 384, 618, 1199, 1663, 3245
BsiWI CGTACG 1 cut(s) 2455
BslFI GGGAC 4 cut(s) 104, 259, 1099, 2886
BslI CCNNNNNNNGG 4 cut(s) 1098, 2371, 2755, 2923
BsmAI GTCTC 5 cut(s) 521, 1868, 1906, 2822, 3028
BsmFI GGGAC 4 cut(s) 104, 259, 1099, 2886
BsmI GAATGC 1 cut(s) 2787
BsnI GGCC 6 cut(s) 357, 383, 1197, 2429, 2667, 3338
BsoBI CYCGRG 5 cut(s) 518, 837, 1198, 2471, 2869
Bsp119I TTCGAA 1 cut(s) 698
Bsp1286I GDGCHC 3 cut(s) 1308, 2382, 3039
Bsp143I GATC 8 cut(s) 468, 592, 715, 1418, 2146, 2245, 3139, 3471
Bsp1720I GCTNAGC 1 cut(s) 2376
BspACI CCGC 4 cut(s) 1089, 1397, 1549, 3490
BspANI GGCC 6 cut(s) 357, 383, 1197, 2429, 2667, 3338
BspCNI CTCAG 9 cut(s) 512, 732, 1882, 1908, 2088, 2145, 2368, 2673, 3187
BspFNI CGCG 2 cut(s) 1167, 2861
BspLI GGNNCC 5 cut(s) 1087, 1667, 2648, 2959, 3016
BspPI GGATC 4 cut(s) 476, 587, 723, 1426
BspQI GCTCTTC 1 cut(s) 2304
BspT104I TTCGAA 1 cut(s) 698
BspT107I GGYRCC 2 cut(s) 1665, 3014
BspTI CTTAAG 3 cut(s) 2249, 2291, 2921
BsrFI RCCGGY 2 cut(s) 617, 1662
BssAI RCCGGY 2 cut(s) 617, 1662
BssECI CCNNGG 7 cut(s) 85, 457, 1092, 1198, 1199, 2303, 3039
BssMI GATC 8 cut(s) 468, 592, 715, 1418, 2146, 2245, 3139, 3471
BssNAI GTATAC 1 cut(s) 2684
BssNI GRCGYC 1 cut(s) 774
BssSI CACGAG 2 cut(s) 1485, 3093
BssT1I CCWWGG 2 cut(s) 1092, 3039
Bst1107I GTATAC 1 cut(s) 2684
Bst2BI CACGAG 2 cut(s) 1485, 3093
Bst2UI CCWGG 4 cut(s) 458, 1764, 2847, 3080
Bst4CI ACNGT 6 cut(s) 18, 495, 1385, 2031, 2210, 2282
Bst6I CTCTTC 5 cut(s) 412, 1866, 1904, 2304, 3150
BstACI GRCGYC 1 cut(s) 774
BstAFI CTTAAG 3 cut(s) 2249, 2291, 2921
BstAPI GCANNNNNTGC 1 cut(s) 1711
BstBAI YACGTR 1 cut(s) 341
BstBI TTCGAA 1 cut(s) 698
BstC8I GCNNGC 7 cut(s) 299, 450, 1320, 1401, 1743, 1994, 3171
BstENI CCTNNNNNAGG 2 cut(s) 1096, 2921
BstFNI CGCG 2 cut(s) 1167, 2861
BstH2I RGCGCY 2 cut(s) 815, 2479
BstHHI GCGC 4 cut(s) 814, 1360, 2478, 2861
BstKTI GATC 8 cut(s) 471, 595, 718, 1421, 2149, 2248, 3142, 3474
BstMAI GTCTC 5 cut(s) 521, 1868, 1906, 2822, 3028
BstMBI GATC 8 cut(s) 468, 592, 715, 1418, 2146, 2245, 3139, 3471
BstMWI GCNNNNNNNGC 9 cut(s) 87, 327, 1088, 1203, 1711, 1993, 2655, 2664, 2856
BstNI CCWGG 4 cut(s) 458, 1764, 2847, 3080
BstNSI RCATGY 5 cut(s) 605, 1322, 1745, 2062, 2803
BstSCI CCNGG 6 cut(s) 456, 1197, 1198, 1762, 2845, 3078
BstSLI GKGCMC 1 cut(s) 3039
BstUI CGCG 2 cut(s) 1167, 2861
BstV1I GCAGC 2 cut(s) 667, 2521
BstV2I GAAGAC 3 cut(s) 339, 475, 2983
BstX2I RGATCY 5 cut(s) 468, 715, 1418, 2245, 3139
BstXI CCANNNNNNTGG 1 cut(s) 1193
BstYI RGATCY 5 cut(s) 468, 715, 1418, 2245, 3139
BstZ17I GTATAC 1 cut(s) 2684
BsuI GTATCC 1 cut(s) 1949
BsuRI GGCC 6 cut(s) 357, 383, 1197, 2429, 2667, 3338
BtgZI GCGATG 1 cut(s) 20
BtsI GCAGTG 1 cut(s) 1078
BtsIMutI CAGTG 2 cut(s) 1078, 2668
Cac8I GCNNGC 7 cut(s) 299, 450, 1320, 1401, 1743, 1994, 3171
CfoI GCGC 4 cut(s) 814, 1360, 2478, 2861
Cfr10I RCCGGY 2 cut(s) 617, 1662
Cfr13I GGNCC 9 cut(s) 355, 1086, 1196, 1285, 1651, 3223, 3336, 3477, 3525
Cfr9I CCCGGG 1 cut(s) 1198
CseI GACGC 1 cut(s) 1122
Csp6I GTAC 8 cut(s) 496, 1124, 1887, 2065, 2456, 3015, 3114, 3263
CspAI ACCGGT 2 cut(s) 617, 1662
CspCI CAANNNNNGTGG 2 cut(s) 1366, 1401
CviQI GTAC 8 cut(s) 496, 1124, 1887, 2065, 2456, 3015, 3114, 3263
DpnI GATC 8 cut(s) 470, 594, 717, 1420, 2148, 2247, 3141, 3473
DpnII GATC 8 cut(s) 468, 592, 715, 1418, 2146, 2245, 3139, 3471
EaeI YGGCCR 1 cut(s) 2427
Eam1104I CTCTTC 5 cut(s) 412, 1866, 1904, 2304, 3150
EarI CTCTTC 5 cut(s) 412, 1866, 1904, 2304, 3150
EciI GGCGGA 1 cut(s) 1564
Ecl136II GAGCTC 1 cut(s) 1306
Eco130I CCWWGG 2 cut(s) 1092, 3039
Eco24I GRGCYC 1 cut(s) 1308
Eco32I GATATC 3 cut(s) 850, 1045, 1735
Eco47I GGWCC 6 cut(s) 1086, 1285, 1651, 3223, 3477, 3525
Eco47III AGCGCT 1 cut(s) 2477
Eco53kI GAGCTC 1 cut(s) 1306
Eco57I CTGAAG 2 cut(s) 307, 711
Eco88I CYCGRG 5 cut(s) 518, 837, 1198, 2471, 2869
EcoICRI GAGCTC 1 cut(s) 1306
EcoNI CCTNNNNNAGG 2 cut(s) 1096, 2921
EcoO109I RGGNCCY 1 cut(s) 1651
EcoRII CCWGG 4 cut(s) 456, 1762, 2845, 3078
EcoRV GATATC 3 cut(s) 850, 1045, 1735
EcoT14I CCWWGG 2 cut(s) 1092, 3039
EcoT22I ATGCAT 1 cut(s) 1707
EcoT38I GRGCYC 1 cut(s) 1308
ErhI CCWWGG 2 cut(s) 1092, 3039
FalI AAGNNNNNCTT 2 cut(s) 1284, 1316
FaqI GGGAC 4 cut(s) 104, 259, 1099, 2886
FbaI TGATCA 1 cut(s) 3471
FblI GTMKAC 3 cut(s) 1146, 2602, 2683
Fnu4HI GCNGC 3 cut(s) 681, 1397, 2510
FriOI GRGCYC 1 cut(s) 1308
Fsp4HI GCNGC 3 cut(s) 681, 1397, 2510
GlaI GCGC 4 cut(s) 813, 1359, 2477, 2860
GluI GCNGC 3 cut(s) 681, 1397, 2510
GsaI CCCAGC 1 cut(s) 715
GsuI CTGGAG 2 cut(s) 490, 1785
HaeII RGCGCY 2 cut(s) 815, 2479
HaeIII GGCC 6 cut(s) 357, 383, 1197, 2429, 2667, 3338
HapII CCGG 6 cut(s) 45, 384, 618, 1199, 1663, 3245
HgaI GACGC 1 cut(s) 1122
HhaI GCGC 4 cut(s) 814, 1360, 2478, 2861
Hin1I GRCGYC 1 cut(s) 774
Hin6I GCGC 4 cut(s) 812, 1358, 2476, 2859
HinP1I GCGC 4 cut(s) 812, 1358, 2476, 2859
HincII GTYRAC 3 cut(s) 2036, 2805, 3007
HindII GTYRAC 3 cut(s) 2036, 2805, 3007
HindIII AAGCTT 3 cut(s) 319, 693, 2675
HpaII CCGG 6 cut(s) 45, 384, 618, 1199, 1663, 3245
HphI GGTGA 8 cut(s) 144, 168, 225, 2278, 2824, 3259, 3374, 3520
Hpy166II GTNNAC 9 cut(s) 258, 1147, 2036, 2230, 2531, 2603, 2684, 2805, 3007
Hpy8I GTNNAC 9 cut(s) 258, 1147, 2036, 2230, 2531, 2603, 2684, 2805, 3007
Hpy99I CGWCG 5 cut(s) 410, 1116, 1526, 3538, 3541
HpyAV CCTTC 9 cut(s) 295, 606, 968, 1835, 2457, 2526, 2660, 2927, 3429
HpyCH4III ACNGT 6 cut(s) 18, 495, 1385, 2031, 2210, 2282
HpyCH4IV ACGT 7 cut(s) 340, 588, 774, 807, 1234, 1938, 3265
HpyF10VI GCNNNNNNNGC 9 cut(s) 87, 327, 1088, 1203, 1711, 1993, 2655, 2664, 2856
HpySE526I ACGT 7 cut(s) 340, 588, 774, 807, 1234, 1938, 3265
Hsp92I GRCGYC 1 cut(s) 774
HspAI GCGC 4 cut(s) 812, 1358, 2476, 2859
KpnI GGTACC 1 cut(s) 3018
Ksp22I TGATCA 1 cut(s) 3471
Kzo9I GATC 8 cut(s) 468, 592, 715, 1418, 2146, 2245, 3139, 3471
LguI GCTCTTC 1 cut(s) 2304
LmnI GCTCC 6 cut(s) 86, 523, 1203, 1311, 1929, 2646
Lsp1109I GCAGC 2 cut(s) 667, 2521
LweI GCATC 4 cut(s) 876, 1692, 1795, 2405
MaeII ACGT 7 cut(s) 340, 588, 774, 807, 1234, 1938, 3265
MaeIII GTNAC 5 cut(s) 12, 775, 1161, 1672, 2404
MalI GATC 8 cut(s) 470, 594, 717, 1420, 2148, 2247, 3141, 3473
MboI GATC 8 cut(s) 468, 592, 715, 1418, 2146, 2245, 3139, 3471
MflI RGATCY 5 cut(s) 468, 715, 1418, 2245, 3139
MhlI GDGCHC 3 cut(s) 1308, 2382, 3039
MlyI GAGTC 5 cut(s) 497, 1873, 2263, 2824, 2868
MmeI TCCRAC 4 cut(s) 805, 1156, 1951, 3168
Mph1103I ATGCAT 1 cut(s) 1707
MroXI GAANNNNTTC 1 cut(s) 1107
MslI CAYNNNNRTG 2 cut(s) 651, 2901
MspA1I CMGCKG 2 cut(s) 510, 1399
MspCI CTTAAG 3 cut(s) 2249, 2291, 2921
MspI CCGG 6 cut(s) 45, 384, 618, 1199, 1663, 3245
MspR9I CCNGG 6 cut(s) 458, 1199, 1200, 1764, 2847, 3080
Mva1269I GAATGC 1 cut(s) 2787
MvaI CCWGG 4 cut(s) 458, 1764, 2847, 3080
MvnI CGCG 2 cut(s) 1167, 2861
MwoI GCNNNNNNNGC 9 cut(s) 87, 327, 1088, 1203, 1711, 1993, 2655, 2664, 2856
NciI CCSGG 2 cut(s) 1199, 1200
NdeII GATC 8 cut(s) 468, 592, 715, 1418, 2146, 2245, 3139, 3471
NlaIV GGNNCC 5 cut(s) 1087, 1667, 2648, 2959, 3016
NmeAIII GCCGAG 2 cut(s) 66, 1887
NmuCI GTSAC 3 cut(s) 775, 1672, 2404
NsiI ATGCAT 1 cut(s) 1707
NspI RCATGY 5 cut(s) 605, 1322, 1745, 2062, 2803
NspV TTCGAA 1 cut(s) 698
PaeI GCATGC 2 cut(s) 1322, 1745
PaeR7I CTCGAG 3 cut(s) 518, 837, 2471
PciSI GCTCTTC 1 cut(s) 2304
PcsI WCGNNNNNNNCGW 1 cut(s) 203
PctI GAATGC 1 cut(s) 2787
PdmI GAANNNNTTC 1 cut(s) 1107
PfeI GAWTC 8 cut(s) 387, 875, 1209, 1480, 1644, 2001, 2122, 2361
Pfl23II CGTACG 1 cut(s) 2455
PflMI CCANNNNNTGG 1 cut(s) 2371
PfoI TCCNGGA 1 cut(s) 1762
PinAI ACCGGT 2 cut(s) 617, 1662
PkrI GCNGC 3 cut(s) 682, 1398, 2511
PleI GAGTC 5 cut(s) 497, 1872, 2262, 2823, 2868
PpsI GAGTC 5 cut(s) 497, 1872, 2262, 2823, 2868
Ppu21I YACGTR 1 cut(s) 341
PpuMI RGGWCCY 1 cut(s) 1651
PshBI ATTAAT 1 cut(s) 1680
PsiI TTATAA 1 cut(s) 951
Psp124BI GAGCTC 1 cut(s) 1308
Psp5II RGGWCCY 1 cut(s) 1651
Psp6I CCWGG 4 cut(s) 456, 1762, 2845, 3078
PspFI CCCAGC 1 cut(s) 711
PspGI CCWGG 4 cut(s) 456, 1762, 2845, 3078
PspLI CGTACG 1 cut(s) 2455
PspN4I GGNNCC 5 cut(s) 1087, 1667, 2648, 2959, 3016
PspPI GGNCC 9 cut(s) 355, 1086, 1196, 1285, 1651, 3223, 3336, 3477, 3525
PspPPI RGGWCCY 1 cut(s) 1651
PspXI VCTCGAGB 2 cut(s) 837, 2471
PsrI GAACNNNNNNTAC 2 cut(s) 782, 814
PsuI RGATCY 5 cut(s) 468, 715, 1418, 2245, 3139
PvuII CAGCTG 1 cut(s) 510
RsaI GTAC 8 cut(s) 497, 1125, 1888, 2066, 2457, 3016, 3115, 3264
RsaNI GTAC 8 cut(s) 496, 1124, 1887, 2065, 2456, 3015, 3114, 3263
RseI CAYNNNNRTG 2 cut(s) 651, 2901
SacI GAGCTC 1 cut(s) 1308
SapI GCTCTTC 1 cut(s) 2304
SatI GCNGC 3 cut(s) 681, 1397, 2510
Sau3AI GATC 8 cut(s) 468, 592, 715, 1418, 2146, 2245, 3139, 3471
Sau96I GGNCC 9 cut(s) 355, 1086, 1196, 1285, 1651, 3223, 3336, 3477, 3525
ScaI AGTACT 1 cut(s) 1888
SchI GAGTC 5 cut(s) 497, 1873, 2263, 2824, 2868
ScrFI CCNGG 6 cut(s) 458, 1199, 1200, 1764, 2847, 3080
SduI GDGCHC 3 cut(s) 1308, 2382, 3039
SfaNI GCATC 4 cut(s) 876, 1692, 1795, 2405
Sfr274I CTCGAG 3 cut(s) 518, 837, 2471
SfuI TTCGAA 1 cut(s) 698
SinI GGWCC 6 cut(s) 1086, 1285, 1651, 3223, 3477, 3525
SlaI CTCGAG 3 cut(s) 518, 837, 2471
SmaI CCCGGG 1 cut(s) 1200
SmiMI CAYNNNNRTG 2 cut(s) 651, 2901
SpeI ACTAGT 2 cut(s) 1877, 3116
SphI GCATGC 2 cut(s) 1322, 1745
SsiI CCGC 4 cut(s) 1089, 1397, 1549, 3490
SstI GAGCTC 1 cut(s) 1308
StyD4I CCNGG 6 cut(s) 456, 1197, 1198, 1762, 2845, 3078
StyI CCWWGG 2 cut(s) 1092, 3039
TaaI ACNGT 6 cut(s) 18, 495, 1385, 2031, 2210, 2282
TaiI ACGT 7 cut(s) 343, 591, 777, 810, 1237, 1941, 3268
TaqII GACCGA 1 cut(s) 1302
TatI WGTACW 2 cut(s) 1886, 2064
TauI GCSGC 1 cut(s) 1399
TfiI GAWTC 8 cut(s) 387, 875, 1209, 1480, 1644, 2001, 2122, 2361
TscAI CASTG 2 cut(s) 1078, 2668
TseFI GTSAC 3 cut(s) 775, 1672, 2404
TseI GCWGC 2 cut(s) 680, 2509
Tsp45I GTSAC 3 cut(s) 775, 1672, 2404
TspGWI ACGGA 1 cut(s) 1786
TspMI CCCGGG 1 cut(s) 1198
TspRI CASTG 2 cut(s) 1078, 2668
Van91I CCANNNNNTGG 1 cut(s) 2371
Vha464I CTTAAG 3 cut(s) 2249, 2291, 2921
VpaK11BI GGWCC 6 cut(s) 1086, 1285, 1651, 3223, 3477, 3525
VspI ATTAAT 1 cut(s) 1680
XagI CCTNNNNNAGG 2 cut(s) 1096, 2921
XbaI TCTAGA 1 cut(s) 2626
XceI RCATGY 5 cut(s) 605, 1322, 1745, 2062, 2803
XhoI CTCGAG 3 cut(s) 518, 837, 2471
XmaI CCCGGG 1 cut(s) 1198
XmiI GTMKAC 3 cut(s) 1146, 2602, 2683
XmnI GAANNNNTTC 1 cut(s) 1107
ZraI GACGTC 1 cut(s) 775
ZrmI AGTACT 1 cut(s) 1888
Zsp2I ATGCAT 1 cut(s) 1707
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.