Prupe.1G550800_v2.0.a1

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0000055
Physical Location & Seq
Reverse (-)
45018636 .. 45024176
5541 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G550800.6

Sequence Viewer

Length: 3165 bp
ATGGATACCAGTACAGCCGTTGAAGCCTCCTCTCCAACCTTCTCATCCTCTTCAACCTGCTCCTTAGACGATGAGATGTACGATGTGTTCTTGAGCTTTAGAGGTGAAGACACACGCAAGACCTTCACCGACCACCTCTACTGGAGGTTAAAACATGCTAGAGTCGACGTGTTTATTGATGAGAATGAAATAAGAGGAGGAGAAATTTTACCAGACGAACTCAAGCAAATAATCGAACGGTCCAGGATCTCTGTCATCATCTTCTCAAGGAGGTATGCGGATTCCATATGGTGTCTTGAGGAGCTGGAGAAGATCATGGAGTGTAGAAGAACACTGGGGCAAATCGTTTTGCCAATTTTCTATGATGTTGATGCTGAAGATGTCAGGAAACAAACTGGTAGTTTTGCAGAAGCATTTCAGACACATGAAGTGCGCTTCCATGGATTTAAAGATGAGGAAGAAAATATACAGTCGTGGAGAAAGTCTCTAACTGAAGCTGCAGGTCTGGACGGCCTTGTTTTTAGCAAGAGTGATGGGTATGAAGGAGTGTTTATCAGGAAAATTATTGACGAGATCAACAGAAAATTAACAACGGTTGCAGTTGGAGTAGATTCTCGCTTGCAAGATATTAGTACTCATTTAGATGTTGGAGGATCAAATGATGTTCGCATAATTGGAATTTGGGGTATGGGCGGAATGGGTAAAACAACGGTTGCAGAAGCTATTTTCAACAAATATCATCCCAGTTTTGATGCTAAAAGTTTCATTCAAAACATGAGGGAAAGGAAACTGGTTGATCTTCAAGAACAACTTCTCTTTGATATCTTGAAACCGACAAAGGCAGAGGGAAGTAGTATGGATGACAGGAGGTATGAGATAAAGAAAAGACTTCCAACAAGAAAGGTACTTGTTATATTTGATGATATCTGTCATGTAGACCAACTAAAGGCATTAGCTATTAAACGTGACTCCTTTGGTCGAGGAAGTAGAATTGTTATAACAACAAGAAACAAACATTTGCTAGAGATAATTGAAGTGGATAAGATATGTAGCCTTAAAGCAATGAATGAAGTAGAAGCTCTTGAGCTACTTAGTTTGCATGCCTTTAAACGTCGTTGTCCAAGTGAAGGATATCTTGAACTCTCAAGAGAAGTTGTTGTTTACTGTGGTGGTTTGCCACTAGCACTGAAAGTTTTAGGCTCTTCTCTATACAATAGAAGCACAACAGAATGGAGAAATGCATTGGATAAATGGAAAAGTCTTCCTCCTAAGGAAATTCACCAAAAGCTTAAATTAAGCTATGATGAGCTACCTGATAATTACCTGAGAGATGCATTCCTTGATATATCATGTTTCTTCATTGGAATGGACATGAACTATGTCATGCGAATATTGGTCCGTCGTGGCTTTTCTCCGGTAATAGGACTGAGTGTCCTCCTTGAAGAGAGCCTCTTAACTGTTGGTGAAGATAACAAGTTGATGATGCATGATTTGGTTCGAGACATGGGCAGAGAAATTGCACGTGCACAATCCCCTAACATCGCTGGAGAACGTAGTAGATTGTGGCATCAGGAAGATGTAAAAGACGTATTGAGGAACAAATCTGGAACAGAGGAAATTGAAGCACTCACTTTAGATTTGCAAGAATCTGAGGATCCTAGCTTCAGTTCCGAAGCATTTAGAAGGATGTGGAGATTGAGATTGCTCAAACTCAATTATGTACAGCTGACTGGAAGCTACAAGCATATTTCCAAAGAGTTAAGATGGTTATGTTGGCATGGATTTCCTCTGGAGGTCATACCAAAAGACTTTTATCAAAAAAACCTAGTTGCTATTGACATGAGCCATAGCAAGCTCATACGAGTTTGGGAGGATTCCCACGTGTTTCTTTCGGAGTTGAAGTGTCTTCATCTCAGCCATTCCGCTTGCCTGAAAGAATTACCCGACTTTTCAAGACTCCCAAATCTCGAGGAATTGATACTCAGAGGCTGTAAGCGTTTGTTATGGGGTCACTATTCCATGATGAGACGGTTGGACAAGCTTAAATTGTTTGATCTTGGGTACTGCAACTTAACTGAAGATAAAGTTGTTAATGAGCTTCGTTTTCTACGTTCTTTAAAAATTTTACGTCTGGATGGAAATGGTTTCGATAGGCTACCTGGGCTCGGTTCTCTTTCCGAACTTGAAGAGTTAACTTTAAATGATTGCAAAAACCTTACACGAATCAGTGATTTACCAAAGACTTTGAAATTCCTGAAAGCGAATTACTGCACTGTACTAAAAAGCATTGAATTTTTGGCAAAGAGGTCGAATATGAGAGAATTGGATCTGAAGGACTGCCGCAACCTCCAGTATACTAAAGCCTTGGGTGACTTGTTACACTCCATGGAAACGATTCATATGGAAGGCTGCACCAATCTCAGTGCTCGGTTTAAGGAGAGAATCCTACAGGGATGGGCCGCGAGCGGAGGGGGTGGCCTTTTCTTCTCTGGAAATGATATTCCTTCGTGGTTCACAGCTGTGGTCAATGAGGATGAAATTGTCTATATCGATGTGCCGAATTCTGGCATAGCTGCCTTGACTGTGTGCATCATTTATTCCTCAGACGACTCGGAGTCTAGTGGGAGATTTTCTTTGACTGTTGCGAATCGTACCCAGCGCACTGCATTTTCCATATTTCCAATGACCGTCTCTGGAGTGACTCCCCATGAAGATTATCTCTGGCTTGGACGTATACCAAACAATGTTCTCAATCTGAAAGGCGGGGACAAGGTTCATGCTCGCGCAGAATTCTTAAGAGAAGAGGGCAAGAAGCATTTAAAGTTGAAGAAAACAGGACTCTGTCTAGAACAGAGTGTTTCTACACATGCAGGTAAGGTACATGAGATGGAGTGGGAGTCTAAACCATATACATATCCTGATACTGATGATGACGCGTGGCCAAGCAAACCCTCGCATAACCTCATATGGAAAACGACACCGTTGTCGGTAAAGGAAATTTACTTGCGACTGGAAGGCAACTTGATGCCTGAAAAAGGAAAGCGAAGGAGTGAAGAGTCTGAAGAAGCGGCTGATGATGACGCGTGGCCAAGCAAACCCTCGCGTGACCTCTTACTAGAGAGTTATCGGAAGAATTACGTGAAGAAGCGTATGAGGCCTGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

1055

Amino Acids

121.15

Weight (kDa)

6.43

Isoelectric Point (pI)

49.75

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000055)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G36930 AT5G36930 AT5G36930 AT5G36930
fragaria_vesca FvH4_3g45361 FvH4_3g45380 FvH4_5g21061 FvH4_5g21062 FvH4_5g21063 FvH4_5g32050 FvH4_5g32970 FvH4_5g32990 FvH4_5g34190 FvH4_5g34210 FvH4_5g34210 FvH4_5g34210 FvH4_5g34210 FvH4_5g34210 FvH4_5g34210 FvH4_5g34211 FvH4_5g34240 FvH4_5g34250
malus_domestica MD00G1011700.v1.1 MD00G1026100.v1.1 MD00G1026500.v1.1 MD00G1027000.v1.1 MD00G1027100.v1.1 MD00G1027700.v1.1 MD00G1027800.v1.1 MD00G1028000.v1.1 MD00G1220000.v1.1 MD02G1024400.v1.1 MD02G1024800.v1.1 MD02G1026000.v1.1 MD02G1026100.v1.1 MD02G1031800.v1.1 MD02G1038500.v1.1 MD02G1038700.v1.1 MD02G1039200.v1.1 MD02G1040600.v1.1 MD02G1040900.v1.1 MD02G1041200.v1.1 MD02G1041300.v1.1 MD02G1041700.v1.1 MD02G1042000.v1.1 MD02G1043800.v1.1 MD02G1043900.v1.1 MD02G1044300.v1.1 MD02G1045200.v1.1 MD02G1045300.v1.1 MD02G1051200.v1.1 MD02G1052200.v1.1 MD02G1052800.v1.1 MD02G1053100.v1.1 MD02G1053600.v1.1 MD02G1055500.v1.1 MD02G1063300.v1.1 MD02G1063400.v1.1 MD02G1063800.v1.1 MD02G1306500.v1.1 MD02G1306600.v1.1 MD02G1306700.v1.1 MD02G1306900.v1.1 MD02G1307200.v1.1 MD04G1011700.v1.1 MD05G1315400.v1.1 MD05G1316000.v1.1 MD05G1316700.v1.1 MD05G1317000.v1.1 MD05G1317300.v1.1 MD05G1317600.v1.1 MD05G1317700.v1.1 MD07G1102800.v1.1 MD07G1238200.v1.1 MD08G1175900.v1.1 MD08G1203600.v1.1 MD09G1024200.v1.1 MD09G1039600.v1.1 MD09G1044800.v1.1 MD09G1045100.v1.1 MD10G1038700.v1.1 MD10G1039000.v1.1 MD10G1039200.v1.1 MD10G1040000.v1.1 MD10G1040200.v1.1 MD10G1040600.v1.1 MD10G1040800.v1.1 MD10G1297300.v1.1 MD12G1247500.v1.1 MD12G1247900.v1.1 MD12G1248300.v1.1 MD12G1248800.v1.1 MD15G1103700.v1.1 MD15G1103800.v1.1 MD15G1104000.v1.1 MD15G1179600.v1.1 MD15G1182500.v1.1 MD16G1068200.v1.1 MD16G1068300.v1.1 MD17G1024600.v1.1 MD17G1024700.v1.1 MD17G1025000.v1.1 MD17G1025900.v1.1
prunus_persica Prupe.1G539800_v2.0.a1 Prupe.1G550800_v2.0.a1 Prupe.1G550800_v2.0.a1 Prupe.1G550800_v2.0.a1 Prupe.1G550800_v2.0.a1 Prupe.1G550800_v2.0.a1 Prupe.1G550800_v2.0.a1 Prupe.1G557100_v2.0.a1 Prupe.1G557200_v2.0.a1 Prupe.1G557200_v2.0.a1 Prupe.1G557300_v2.0.a1
pyrus_communis pycom02g02080 pycom02g02090 pycom02g02110 pycom02g03220 pycom02g03260 pycom02g03370 pycom02g03390 pycom02g03410 pycom02g03420 pycom02g03450 pycom02g03500 pycom02g03730 pycom02g03770 pycom02g04400 pycom02g04410 pycom02g04430 pycom02g04450 pycom02g04460 pycom02g04490 pycom02g05200 pycom02g25710 pycom02g25750 pycom02g25790 pycom02g25810 pycom02g25830 pycom04g00920 pycom04g00930 pycom04g00940 pycom05g29300 pycom05g29310 pycom05g29380 pycom05g29410 pycom07g06310 pycom07g21420 pycom08g15070 pycom08g17540 pycom08g17560 pycom08g17570 pycom10g02670 pycom10g02680 pycom10g02700 pycom10g02770 pycom10g02790 pycom10g02800 pycom10g02810 pycom10g25020 pycom10g25030 pycom10g25050 pycom10g25080 pycom111g01990 pycom111g02000 pycom111g02030 pycom111g02040 pycom111g02050 pycom111g02060 pycom12g22710 pycom12g22720 pycom12g22730 pycom12g22740 pycom12g22750 pycom12g22770 pycom12g22780 pycom12g22790 pycom12g22800 pycom12g22810 pycom12g22830 pycom12g22840 pycom12g22860 pycom15g09440 pycom15g09480 pycom15g16120 pycom15g32380 pycom16g05970 pycom17g02100
rosa_chinensis RchiOBHm_Chr2g0144661 RchiOBHm_Chr4g0407491 RchiOBHm_Chr4g0407501 RchiOBHm_Chr4g0412381 RchiOBHm_Chr7g0208191 RchiOBHm_Chr7g0208201 RchiOBHm_Chr7g0208211 RchiOBHm_Chr7g0208221 RchiOBHm_Chr7g0228071 RchiOBHm_Chr7g0228121 RchiOBHm_Chr7g0230971 RchiOBHm_Chr7g0230981 RchiOBHm_Chr7g0233901 RchiOBHm_Chr7g0233951 RchiOBHm_Chr7g0233961 RchiOBHm_Chr7g0233991 RchiOBHm_Chr7g0234021 RchiOBHm_Chr7g0234151 RchiOBHm_Chr7g0234161 RchiOBHm_Chr7g0234241 RchiOBHm_Chr7g0234501 RchiOBHm_Chr7g0234641 RchiOBHm_Chr7g0234771 RchiOBHm_Chr7g0234781 RchiOBHm_Chr7g0234991 RchiOBHm_Chr7g0241291 RchiOBHm_Chr7g0241301
rosa_laevigata RLG00000001186 RLG00000001187 RLG00000001212 RLG00000001226 RLG00000001233 RLG00000001590 RLG00000003242 RLG00000003243 RLG00000003249 RLG00000008688 RLG00000008690
rosa_multiflora Rmu_co7966574.1_g000001 Rmu_co8120830.1_g000001 Rmu_co8142226.1_g000001 Rmu_co8293083.1_g000001 Rmu_co8351227.1_g000001 Rmu_co8380159.1_g000001 Rmu_co8456505.1_g000001 Rmu_sc0000676.1_g000025 Rmu_sc0000706.1_g000018 Rmu_sc0000706.1_g000040 Rmu_sc0000740.1_g000029 Rmu_sc0000740.1_g000032 Rmu_sc0000740.1_g000037 Rmu_sc0000740.1_g000040 Rmu_sc0000762.1_g000003 Rmu_sc0000762.1_g000019 Rmu_sc0000762.1_g000034 Rmu_sc0000762.1_g000069 Rmu_sc0000892.1_g000001 Rmu_sc0000945.1_g000040 Rmu_sc0001913.1_g000037 Rmu_sc0001913.1_g000041 Rmu_sc0001913.1_g000049 Rmu_sc0002775.1_g000017 Rmu_sc0002775.1_g000018 Rmu_sc0002775.1_g000020 Rmu_sc0002775.1_g000021 Rmu_sc0002775.1_g000022 Rmu_sc0002775.1_g000025 Rmu_sc0002775.1_g000026 Rmu_sc0002775.1_g000028 Rmu_sc0002775.1_g000030 Rmu_sc0004858.1_g000002 Rmu_sc0004864.1_g000022 Rmu_sc0005038.1_g000023 Rmu_sc0005840.1_g000014 Rmu_sc0006724.1_g000003 Rmu_sc0006824.1_g000005 Rmu_sc0007869.1_g000003 Rmu_sc0007869.1_g000005 Rmu_sc0007869.1_g000008 Rmu_sc0008646.1_g000007 Rmu_sc0009637.1_g000009 Rmu_sc0010500.1_g000002 Rmu_sc0010500.1_g000012 Rmu_sc0012403.1_g000025 Rmu_sc0013768.1_g000025 Rmu_sc0015625.1_g000001 Rmu_sc0019715.1_g000001 Rmu_sc0021133.1_g000002 Rmu_sc0021904.1_g000001 Rmu_sc0021904.1_g000003 Rmu_sc0021904.1_g000004 Rmu_sc0022054.1_g000001 Rmu_sc0026025.1_g000001 Rmu_sc0036828.1_g000002 Rmu_sc0041808.1_g000001 Rmu_ssc0000012.1_g000001
rosa_roxburghii Rroxscaffold_3G00226370 Rroxscaffold_3G00226860 Rroxscaffold_3G00226900 Rroxscaffold_3G00226930 Rroxscaffold_3G00226940 Rroxscaffold_3G00226970 Rroxscaffold_3G00226980 Rroxscaffold_3G00231530 Rroxscaffold_3G00237090 Rroxscaffold_3G00250760 Rroxscaffold_3G00250800
rosa_rugosa Rorug04G0082300 Rorug07G0101800 Rorug07G0102000 Rorug07G0102100 Rorug07G0221200 Rorug07G0245700 Rorug07G0245700 Rorug07G0245700 Rorug07G0263900 Rorug07G0264000 Rorug07G0264000 Rorug07G0264500 Rorug07G0282900 Rorug07G0282900 Rorug07G0283100 Rorug07G0283200.1 Rorug07G0283300 Rorug07G0283900 Rorug07G0284000 Rorug07G0284100 Rorug07G0284300 Rorug07G0284400 Rorug07G0284500 Rorug07G0285000 Rorug07G0285200 Rorug07G0285300 Rorug07G0285500 Rorug07G0286800 Rorug07G0286900 Rorug07G0287100 Rorug07G0287200
rosa_samantha Rh7AG236100 Rh7AG236200 Rh7AG236300 Rh7AG440000 Rh7AG440100 Rh7BG411900 Rh7BG412000 Rh7BG412600 Rh7CG251300 Rh7CG417700 Rh7CG460100 Rh7DG242100 Rh7DG242200 Rh7DG242300 Rh7DG429300
rosa_wichuraiana Rw0G005070 Rw0G010780 Rw0G012530 Rw7G020110 Rw7G020160 Rw7G020170 Rw7G033210 Rw7G033230 Rw7G033250 Rw7G034540 Rw7G035150 Rw7G036190 Rw7G036210 Rw7G036260 Rw7G036270 Rw7G036290 Rw7G036300 Rw7G036310 Rw7G036350 Rw7G036360 Rw7G036440 Rw7G036510 Rw7G036530 Rw7G036570 Rw7G036590 Rw7G036730

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 998
AasI GACNNNNNNGTC 1 cut(s) 2983
Acc36I ACCTGC 3 cut(s) 65, 491, 2861
AccBSI CCGCTC 1 cut(s) 2466
AccI GTMKAC 4 cut(s) 165, 936, 2354, 2734
AccII CGCG 5 cut(s) 2462, 2784, 2936, 3083, 3103
AciI CCGC 8 cut(s) 278, 693, 1923, 2341, 2460, 2466, 2763, 3068
AclWI GGATC 5 cut(s) 254, 661, 1649, 1662, 2334
AcoI YGGCCR 2 cut(s) 2939, 3086
AcsI RAATTY 9 cut(s) 204, 678, 1275, 2121, 2249, 2291, 2560, 2789, 2997
AcuI CTGAAG 6 cut(s) 396, 513, 1648, 2097, 2351, 3081
AcvI CACGTG 2 cut(s) 1523, 1882
AfaI GTAC 9 cut(s) 13, 80, 634, 906, 1722, 2063, 2277, 2653, 2880
AfiI CCNNNNNNNGG 6 cut(s) 946, 1127, 1421, 2165, 2564, 3035
AflII CTTAAG 1 cut(s) 2794
AflIII ACRYGT 4 cut(s) 168, 1881, 2934, 3081
AhdI GACNNNNNGTC 2 cut(s) 1430, 2614
AjiI CACGTC 1 cut(s) 169
AjnI CCWGG 2 cut(s) 242, 2158
AleI CACNNNNGTG 1 cut(s) 2519
Alw21I GWGCWC 2 cut(s) 1528, 2428
Alw26I GTCTC 4 cut(s) 489, 1494, 2020, 2695
Alw44I GTGCAC 1 cut(s) 1524
AlwI GGATC 5 cut(s) 254, 661, 1649, 1662, 2334
AlwNI CAGNNNCTG 1 cut(s) 1989
Ama87I CYCGRG 1 cut(s) 1967
AoxI GGCC 6 cut(s) 511, 2457, 2476, 2939, 3086, 3155
ApaLI GTGCAC 1 cut(s) 1524
ApeKI GCWGC 3 cut(s) 497, 2409, 2573
ApoI RAATTY 9 cut(s) 204, 678, 1275, 2121, 2249, 2291, 2560, 2789, 2997
Asp700I GAANNNNTTC 3 cut(s) 414, 810, 2394
AspLEI GCGC 3 cut(s) 435, 2661, 2786
AspS9I GGNCC 3 cut(s) 240, 1396, 2457
AsuHPI GGTGA 5 cut(s) 116, 118, 1271, 1475, 2381
AvaI CYCGRG 1 cut(s) 1967
AvaII GGWCC 2 cut(s) 240, 1396
AxyI CCTNAGG 1 cut(s) 1269
BaeGI GKGCMC 1 cut(s) 1528
BalI TGGCCA 2 cut(s) 2941, 3088
BamHI GGATCC 1 cut(s) 1654
BanII GRGCYC 1 cut(s) 2166
BarI GAAGNNNNNNTAC 2 cut(s) 450, 482
BbrPI CACGTG 2 cut(s) 1523, 1882
BbsI GAAGAC 3 cut(s) 114, 1253, 1898
Bbv12I GWGCWC 2 cut(s) 1528, 2428
BbvI GCAGC 3 cut(s) 484, 2396, 2560
BccI CCATC 5 cut(s) 527, 1758, 2129, 2448, 2881
BceAI ACGGC 2 cut(s) 2, 526
BcgI CGANNNNNNTGC 2 cut(s) 2289, 2323
BciT130I CCWGG 2 cut(s) 244, 2160
BcoDI GTCTC 4 cut(s) 489, 1494, 2020, 2695
BfaI CTAG 8 cut(s) 159, 1022, 1181, 1659, 1826, 2619, 2846, 3116
BfmI CTRYAG 2 cut(s) 498, 2447
BfrI CTTAAG 1 cut(s) 2794
BfuAI ACCTGC 3 cut(s) 65, 491, 2861
BisI GCNGC 6 cut(s) 498, 2341, 2410, 2460, 2574, 3069
BlsI GCNGC 6 cut(s) 499, 2342, 2411, 2461, 2575, 3070
BmcAI AGTACT 1 cut(s) 634
Bme1390I CCNGG 2 cut(s) 244, 2160
Bme18I GGWCC 2 cut(s) 240, 1396
BmeRI GACNNNNNGTC 2 cut(s) 1430, 2614
BmeT110I CYCGRG 1 cut(s) 1967
BmgBI CACGTC 1 cut(s) 169
BmgT120I GGNCC 3 cut(s) 240, 1396, 2457
BmiI GGNNCC 1 cut(s) 1656
BmrFI CCNGG 2 cut(s) 244, 2160
BmrI ACTGGG 2 cut(s) 344, 738
BmsI GCATC 7 cut(s) 361, 742, 1321, 1473, 1576, 2598, 3015
BmuI ACTGGG 2 cut(s) 344, 738
BpiI GAAGAC 3 cut(s) 114, 1253, 1898
BplI GAGNNNNNCTC 2 cut(s) 469, 501
BpmI CTGGAG 6 cut(s) 163, 326, 1566, 1811, 2333, 2715
BpuEI CTTGAG 6 cut(s) 112, 206, 250, 317, 1103, 1129
Bsa29I ATCGAT 1 cut(s) 2550
BsaAI YACGTR 3 cut(s) 1523, 1882, 3139
BsaJI CCNNGG 4 cut(s) 439, 2159, 2364, 2385
BsaWI WCCGGW 1 cut(s) 1414
BsaXI ACNNNNNCTCC 4 cut(s) 1886, 1916, 2882, 2912
Bsc4I CCNNNNNNNGG 6 cut(s) 946, 1127, 1421, 2165, 2564, 3035
Bse1I ACTGG 9 cut(s) 9, 146, 339, 400, 744, 795, 1735, 2350, 3015
Bse21I CCTNAGG 1 cut(s) 1269
Bse3DI GCAATG 1 cut(s) 1068
BseBI CCWGG 2 cut(s) 244, 2160
BseCI ATCGAT 1 cut(s) 2550
BseDI CCNNGG 4 cut(s) 439, 2159, 2364, 2385
BseGI GGATG 7 cut(s) 44, 739, 865, 1692, 2140, 2459, 2539
BseLI CCNNNNNNNGG 6 cut(s) 946, 1127, 1421, 2165, 2564, 3035
BseMI GCAATG 1 cut(s) 1068
BseMII CTCAG 7 cut(s) 1316, 1418, 1641, 1927, 1996, 2434, 2616
BseNI ACTGG 9 cut(s) 9, 146, 339, 400, 744, 795, 1735, 2350, 3015
BseRI GAGGAG 4 cut(s) 19, 210, 213, 314
BseSI GKGCMC 1 cut(s) 1528
BseXI GCAGC 3 cut(s) 484, 2396, 2560
BseYI CCCAGC 1 cut(s) 2655
BsgI GTGCAG 2 cut(s) 2254, 2395
Bsh1236I CGCG 5 cut(s) 2462, 2784, 2936, 3083, 3103
BshFI GGCC 6 cut(s) 513, 2459, 2478, 2941, 3088, 3157
BshVI ATCGAT 1 cut(s) 2550
BsiHKAI GWGCWC 2 cut(s) 1528, 2428
BsiHKCI CYCGRG 1 cut(s) 1967
BsiSI CCGG 1 cut(s) 1415
BslFI GGGAC 1 cut(s) 2780
BslI CCNNNNNNNGG 6 cut(s) 946, 1127, 1421, 2165, 2564, 3035
BsmAI GTCTC 4 cut(s) 489, 1494, 2020, 2695
BsmBI CGTCTC 2 cut(s) 2020, 2695
BsmFI GGGAC 1 cut(s) 2780
BsmI GAATGC 1 cut(s) 1334
BsnI GGCC 6 cut(s) 513, 2459, 2478, 2941, 3088, 3157
BsoBI CYCGRG 1 cut(s) 1967
Bsp1286I GDGCHC 3 cut(s) 1528, 2166, 2428
Bsp1407I TGTACA 1 cut(s) 1720
Bsp143I GATC 8 cut(s) 246, 312, 573, 653, 796, 1654, 2053, 2326
Bsp19I CCATGG 2 cut(s) 439, 2385
BspACI CCGC 8 cut(s) 278, 693, 1923, 2341, 2460, 2466, 2763, 3068
BspANI GGCC 6 cut(s) 513, 2459, 2478, 2941, 3088, 3157
BspCNI CTCAG 7 cut(s) 1317, 1419, 1642, 1926, 1995, 2433, 2615
BspDI ATCGAT 1 cut(s) 2550
BspFNI CGCG 5 cut(s) 2462, 2784, 2936, 3083, 3103
BspLI GGNNCC 1 cut(s) 1656
BspMAI CTGCAG 1 cut(s) 502
BspMI ACCTGC 3 cut(s) 65, 491, 2861
BspPI GGATC 5 cut(s) 254, 661, 1649, 1662, 2334
BspQI GCTCTTC 1 cut(s) 1207
BspTI CTTAAG 1 cut(s) 2794
BsrBI CCGCTC 1 cut(s) 2466
BsrDI GCAATG 1 cut(s) 1068
BsrGI TGTACA 1 cut(s) 1720
BsrI ACTGG 9 cut(s) 9, 146, 339, 400, 744, 795, 1735, 2350, 3015
BssECI CCNNGG 4 cut(s) 439, 2159, 2364, 2385
BssMI GATC 8 cut(s) 246, 312, 573, 653, 796, 1654, 2053, 2326
BssNAI GTATAC 2 cut(s) 2355, 2735
BssT1I CCWWGG 3 cut(s) 439, 2364, 2385
Bst1107I GTATAC 2 cut(s) 2355, 2735
Bst2UI CCWGG 2 cut(s) 244, 2160
Bst6I CTCTTC 6 cut(s) 55, 1207, 1437, 2181, 2796, 3048
BstAFI CTTAAG 1 cut(s) 2794
BstAUI TGTACA 1 cut(s) 1720
BstBAI YACGTR 3 cut(s) 1523, 1882, 3139
BstC8I GCNNGC 6 cut(s) 620, 1101, 1853, 1927, 2464, 2782
BstDSI CCRYGG 2 cut(s) 439, 2385
BstENI CCTNNNNNAGG 1 cut(s) 3033
BstF5I GGATG 7 cut(s) 44, 739, 865, 1692, 2140, 2459, 2539
BstFNI CGCG 5 cut(s) 2462, 2784, 2936, 3083, 3103
BstHHI GCGC 3 cut(s) 435, 2661, 2786
BstKTI GATC 8 cut(s) 249, 315, 576, 656, 799, 1657, 2056, 2329
BstMAI GTCTC 4 cut(s) 489, 1494, 2020, 2695
BstMBI GATC 8 cut(s) 246, 312, 573, 653, 796, 1654, 2053, 2326
BstMWI GCNNNNNNNGC 3 cut(s) 23, 2161, 3154
BstNI CCWGG 2 cut(s) 244, 2160
BstNSI RCATGY 3 cut(s) 158, 1103, 2870
BstSCI CCNGG 2 cut(s) 242, 2158
BstSFI CTRYAG 2 cut(s) 498, 2447
BstSLI GKGCMC 1 cut(s) 1528
BstUI CGCG 5 cut(s) 2462, 2784, 2936, 3083, 3103
BstV1I GCAGC 3 cut(s) 484, 2396, 2560
BstV2I GAAGAC 3 cut(s) 114, 1253, 1898
BstX2I RGATCY 3 cut(s) 246, 1654, 2326
BstYI RGATCY 3 cut(s) 246, 1654, 2326
BstZ17I GTATAC 2 cut(s) 2355, 2735
Bsu15I ATCGAT 1 cut(s) 2550
Bsu36I CCTNAGG 1 cut(s) 1269
BsuRI GGCC 6 cut(s) 513, 2459, 2478, 2941, 3088, 3157
BsuTUI ATCGAT 1 cut(s) 2550
BtgI CCRYGG 2 cut(s) 439, 2385
BtgZI GCGATG 1 cut(s) 1525
BtrI CACGTC 1 cut(s) 169
BtsCI GGATG 7 cut(s) 44, 739, 865, 1692, 2140, 2459, 2539
BtsI GCAGTG 1 cut(s) 2661
BtsIMutI CAGTG 6 cut(s) 332, 1184, 2233, 2271, 2428, 2661
BveI ACCTGC 3 cut(s) 65, 491, 2861
Cac8I GCNNGC 6 cut(s) 620, 1101, 1853, 1927, 2464, 2782
CaiI CAGNNNCTG 1 cut(s) 1989
CfoI GCGC 3 cut(s) 435, 2661, 2786
Cfr13I GGNCC 3 cut(s) 240, 1396, 2457
ClaI ATCGAT 1 cut(s) 2550
CseI GACGC 2 cut(s) 2942, 3089
Csp6I GTAC 9 cut(s) 12, 79, 633, 905, 1721, 2062, 2276, 2652, 2879
CviQI GTAC 9 cut(s) 12, 79, 633, 905, 1721, 2062, 2276, 2652, 2879
DpnI GATC 8 cut(s) 248, 314, 575, 655, 798, 1656, 2055, 2328
DpnII GATC 8 cut(s) 246, 312, 573, 653, 796, 1654, 2053, 2326
DraI TTTAAA 5 cut(s) 448, 1108, 2118, 2199, 2820
DrdI GACNNNNNNGTC 1 cut(s) 2983
DriI GACNNNNNGTC 2 cut(s) 1430, 2614
DseDI GACNNNNNNGTC 1 cut(s) 2983
EaeI YGGCCR 2 cut(s) 2939, 3086
Eam1104I CTCTTC 6 cut(s) 55, 1207, 1437, 2181, 2796, 3048
Eam1105I GACNNNNNGTC 2 cut(s) 1430, 2614
EarI CTCTTC 6 cut(s) 55, 1207, 1437, 2181, 2796, 3048
EciI GGCGGA 1 cut(s) 708
Eco130I CCWWGG 3 cut(s) 439, 2364, 2385
Eco147I AGGCCT 1 cut(s) 3157
Eco24I GRGCYC 1 cut(s) 2166
Eco32I GATATC 3 cut(s) 823, 925, 1133
Eco47I GGWCC 2 cut(s) 240, 1396
Eco57I CTGAAG 6 cut(s) 396, 513, 1648, 2097, 2351, 3081
Eco72I CACGTG 2 cut(s) 1523, 1882
Eco81I CCTNAGG 1 cut(s) 1269
Eco88I CYCGRG 1 cut(s) 1967
EcoNI CCTNNNNNAGG 1 cut(s) 3033
EcoRI GAATTC 2 cut(s) 2560, 2789
EcoRII CCWGG 2 cut(s) 242, 2158
EcoRV GATATC 3 cut(s) 823, 925, 1133
EcoT14I CCWWGG 3 cut(s) 439, 2364, 2385
EcoT22I ATGCAT 3 cut(s) 1243, 1336, 1488
EcoT38I GRGCYC 1 cut(s) 2166
ErhI CCWWGG 3 cut(s) 439, 2364, 2385
Esp3I CGTCTC 2 cut(s) 2020, 2695
FalI AAGNNNNNCTT 4 cut(s) 795, 827, 1119, 1151
FaqI GGGAC 1 cut(s) 2780
FauI CCCGC 1 cut(s) 2756
FauNDI CATATG 3 cut(s) 287, 2400, 2966
FblI GTMKAC 4 cut(s) 165, 936, 2354, 2734
Fnu4HI GCNGC 6 cut(s) 498, 2341, 2410, 2460, 2574, 3069
FokI GGATG 7 cut(s) 31, 726, 872, 1699, 2147, 2466, 2546
FriOI GRGCYC 1 cut(s) 2166
Fsp4HI GCNGC 6 cut(s) 498, 2341, 2410, 2460, 2574, 3069
FspBI CTAG 8 cut(s) 159, 1022, 1181, 1659, 1826, 2619, 2846, 3116
GlaI GCGC 3 cut(s) 434, 2660, 2785
GluI GCNGC 6 cut(s) 498, 2341, 2410, 2460, 2574, 3069
GsaI CCCAGC 1 cut(s) 2659
GsuI CTGGAG 6 cut(s) 163, 326, 1566, 1811, 2333, 2715
HaeIII GGCC 6 cut(s) 513, 2459, 2478, 2941, 3088, 3157
HapII CCGG 1 cut(s) 1415
HgaI GACGC 2 cut(s) 2942, 3089
HhaI GCGC 3 cut(s) 435, 2661, 2786
Hin6I GCGC 3 cut(s) 433, 2659, 2784
HinP1I GCGC 3 cut(s) 433, 2659, 2784
HincII GTYRAC 2 cut(s) 166, 2193
HindII GTYRAC 2 cut(s) 166, 2193
HindIII AAGCTT 2 cut(s) 1286, 2039
HpaI GTTAAC 1 cut(s) 2193
HpaII CCGG 1 cut(s) 1415
HphI GGTGA 5 cut(s) 116, 118, 1271, 1475, 2381
Hpy166II GTNNAC 8 cut(s) 166, 937, 1162, 1526, 2193, 2355, 2514, 2735
Hpy8I GTNNAC 8 cut(s) 166, 937, 1162, 1526, 2193, 2355, 2514, 2735
Hpy99I CGWCG 3 cut(s) 170, 1116, 1404
HpyF10VI GCNNNNNNNGC 3 cut(s) 23, 2161, 3154
HspAI GCGC 3 cut(s) 433, 2659, 2784
KspAI GTTAAC 1 cut(s) 2193
Kzo9I GATC 8 cut(s) 246, 312, 573, 653, 796, 1654, 2053, 2326
LguI GCTCTTC 1 cut(s) 1207
LmnI GCTCC 2 cut(s) 65, 301
Lsp1109I GCAGC 3 cut(s) 484, 2396, 2560
LweI GCATC 7 cut(s) 361, 742, 1321, 1473, 1576, 2598, 3015
MaeI CTAG 8 cut(s) 159, 1022, 1181, 1659, 1826, 2619, 2846, 3116
MaeIII GTNAC 6 cut(s) 965, 2009, 2369, 2376, 2698, 3104
MalI GATC 8 cut(s) 248, 314, 575, 655, 798, 1656, 2055, 2328
MbiI CCGCTC 1 cut(s) 2466
MboI GATC 8 cut(s) 246, 312, 573, 653, 796, 1654, 2053, 2326
MflI RGATCY 3 cut(s) 246, 1654, 2326
MhlI GDGCHC 3 cut(s) 1528, 2166, 2428
MlsI TGGCCA 2 cut(s) 2941, 3088
MluI ACGCGT 2 cut(s) 2934, 3081
MluNI TGGCCA 2 cut(s) 2941, 3088
MlyI GAGTC 9 cut(s) 171, 962, 1950, 2603, 2624, 2695, 2832, 2906, 3065
MmeI TCCRAC 5 cut(s) 59, 583, 628, 917, 2013
Mox20I TGGCCA 2 cut(s) 2941, 3088
Mph1103I ATGCAT 3 cut(s) 1243, 1336, 1488
MroXI GAANNNNTTC 3 cut(s) 414, 810, 2394
MscI TGGCCA 2 cut(s) 2941, 3088
MslI CAYNNNNRTG 3 cut(s) 642, 1364, 2519
Msp20I TGGCCA 2 cut(s) 2941, 3088
MspA1I CMGCKG 2 cut(s) 1726, 2519
MspCI CTTAAG 1 cut(s) 2794
MspI CCGG 1 cut(s) 1415
MspR9I CCNGG 2 cut(s) 244, 2160
Mva1269I GAATGC 1 cut(s) 1334
MvaI CCWGG 2 cut(s) 244, 2160
MvnI CGCG 5 cut(s) 2462, 2784, 2936, 3083, 3103
MwoI GCNNNNNNNGC 3 cut(s) 23, 2161, 3154
NcoI CCATGG 2 cut(s) 439, 2385
NdeI CATATG 3 cut(s) 287, 2400, 2966
NdeII GATC 8 cut(s) 246, 312, 573, 653, 796, 1654, 2053, 2326
NlaIV GGNNCC 1 cut(s) 1656
NmuCI GTSAC 5 cut(s) 965, 2009, 2369, 2698, 3104
NsiI ATGCAT 3 cut(s) 1243, 1336, 1488
NspI RCATGY 3 cut(s) 158, 1103, 2870
OliI CACNNNNGTG 1 cut(s) 2519
PaeI GCATGC 1 cut(s) 1103
PaeR7I CTCGAG 1 cut(s) 1967
PceI AGGCCT 1 cut(s) 3157
PciSI GCTCTTC 1 cut(s) 1207
PcsI WCGNNNNNNNCGW 1 cut(s) 2107
PctI GAATGC 1 cut(s) 1334
PdmI GAANNNNTTC 3 cut(s) 414, 810, 2394
PfeI GAWTC 8 cut(s) 281, 611, 1646, 1874, 2223, 2395, 2442, 2647
PflFI GACNNNGTC 1 cut(s) 2841
PfoI TCCNGGA 1 cut(s) 242
PkrI GCNGC 6 cut(s) 499, 2342, 2411, 2461, 2575, 3070
PleI GAGTC 9 cut(s) 170, 962, 1950, 2603, 2623, 2695, 2832, 2905, 3064
PmaCI CACGTG 2 cut(s) 1523, 1882
PmlI CACGTG 2 cut(s) 1523, 1882
PpsI GAGTC 9 cut(s) 170, 962, 1950, 2603, 2623, 2695, 2832, 2905, 3064
Ppu21I YACGTR 3 cut(s) 1523, 1882, 3139
PsiI TTATAA 1 cut(s) 998
Psp6I CCWGG 2 cut(s) 242, 2158
PspCI CACGTG 2 cut(s) 1523, 1882
PspFI CCCAGC 1 cut(s) 2655
PspGI CCWGG 2 cut(s) 242, 2158
PspN4I GGNNCC 1 cut(s) 1656
PspPI GGNCC 3 cut(s) 240, 1396, 2457
PsrI GAACNNNNNNTAC 2 cut(s) 71, 103
PstI CTGCAG 1 cut(s) 502
PstNI CAGNNNCTG 1 cut(s) 1989
PsuI RGATCY 3 cut(s) 246, 1654, 2326
PsyI GACNNNGTC 1 cut(s) 2841
PvuII CAGCTG 2 cut(s) 1726, 2519
RsaI GTAC 9 cut(s) 13, 80, 634, 906, 1722, 2063, 2277, 2653, 2880
RsaNI GTAC 9 cut(s) 12, 79, 633, 905, 1721, 2062, 2276, 2652, 2879
RseI CAYNNNNRTG 3 cut(s) 642, 1364, 2519
SalI GTCGAC 1 cut(s) 164
SapI GCTCTTC 1 cut(s) 1207
SatI GCNGC 6 cut(s) 498, 2341, 2410, 2460, 2574, 3069
Sau3AI GATC 8 cut(s) 246, 312, 573, 653, 796, 1654, 2053, 2326
Sau96I GGNCC 3 cut(s) 240, 1396, 2457
ScaI AGTACT 1 cut(s) 634
SchI GAGTC 9 cut(s) 171, 962, 1950, 2603, 2624, 2695, 2832, 2906, 3065
ScrFI CCNGG 2 cut(s) 244, 2160
SduI GDGCHC 3 cut(s) 1528, 2166, 2428
SfaNI GCATC 7 cut(s) 361, 742, 1321, 1473, 1576, 2598, 3015
SfcI CTRYAG 2 cut(s) 498, 2447
Sfr274I CTCGAG 1 cut(s) 1967
SinI GGWCC 2 cut(s) 240, 1396
SlaI CTCGAG 1 cut(s) 1967
SmiMI CAYNNNNRTG 3 cut(s) 642, 1364, 2519
SmlI CTYRAG 8 cut(s) 91, 221, 265, 296, 1082, 1144, 1967, 2794
SmoI CTYRAG 8 cut(s) 91, 221, 265, 296, 1082, 1144, 1967, 2794
SphI GCATGC 1 cut(s) 1103
SseBI AGGCCT 1 cut(s) 3157
SsiI CCGC 8 cut(s) 278, 693, 1923, 2341, 2460, 2466, 2763, 3068
SspI AATATT 1 cut(s) 1392
SspMI CTAG 8 cut(s) 159, 1022, 1181, 1659, 1826, 2619, 2846, 3116
StuI AGGCCT 1 cut(s) 3157
StyD4I CCNGG 2 cut(s) 242, 2158
StyI CCWWGG 3 cut(s) 439, 2364, 2385
TaqI TCGA 8 cut(s) 165, 234, 979, 1498, 1968, 2148, 2309, 2550
TatI WGTACW 4 cut(s) 11, 632, 1720, 2275
TauI GCSGC 3 cut(s) 2343, 2462, 3071
TfiI GAWTC 8 cut(s) 281, 611, 1646, 1874, 2223, 2395, 2442, 2647
TscAI CASTG 6 cut(s) 339, 1191, 2233, 2278, 2428, 2668
TseFI GTSAC 5 cut(s) 965, 2009, 2369, 2698, 3104
TseI GCWGC 3 cut(s) 497, 2409, 2573
Tsp45I GTSAC 5 cut(s) 965, 2009, 2369, 2698, 3104
TspGWI ACGGA 1 cut(s) 1388
TspRI CASTG 6 cut(s) 339, 1191, 2233, 2278, 2428, 2668
Tth111I GACNNNGTC 1 cut(s) 2841
Vha464I CTTAAG 1 cut(s) 2794
VneI GTGCAC 1 cut(s) 1524
VpaK11BI GGWCC 2 cut(s) 240, 1396
XagI CCTNNNNNAGG 1 cut(s) 3033
XapI RAATTY 9 cut(s) 204, 678, 1275, 2121, 2249, 2291, 2560, 2789, 2997
XbaI TCTAGA 1 cut(s) 2845
XceI RCATGY 3 cut(s) 158, 1103, 2870
XhoI CTCGAG 1 cut(s) 1967
XmiI GTMKAC 4 cut(s) 165, 936, 2354, 2734
XmnI GAANNNNTTC 3 cut(s) 414, 810, 2394
XspI CTAG 8 cut(s) 159, 1022, 1181, 1659, 1826, 2619, 2846, 3116
ZrmI AGTACT 1 cut(s) 634
Zsp2I ATGCAT 3 cut(s) 1243, 1336, 1488
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.