Rmu_sc0004864.1_g000022

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0004864.1
OG0000055
Physical Location & Seq
Forward (+)
66302 .. 71194
4893 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0004864.1_g000022.1.cds

Sequence Viewer

Length: 2769 bp
atgaaggcatcgtcatcatcctcatcgcttccatcggggacaccatcctcaaggccaccggagttccttaactacacttacgacgtgttcttgagcttcagaggtgaagacacacggaaggccttcacagaccacctctacacggcactagtttgtgccggagtcaggacgtttagggatgccgagggactaagaaggggagaaaacatagcggaggatttggtggagataatccaagggtgtaggatctcactcatcgtcttctcagaaaattacgcggattcgagttggtgtctcgaggagctggtgcagatcatggagtgtagaaaaacacaaaggcaacgggttttcccaatattctatcatgtcgatccttcacatgtcagaaaccagaccggtatctttgctcatgcgtttgagaaacatgaagccagagtacatcaaggtagagataagatagcaaggtggagagctgctcttagaggagcggcggatttggctggcttcgatattgctgataggcatgaagcagagtttatcaatagcattgttgacgagatatgcagacagctgaccaccccatacttgaatttagctctctacccagttggaatagagtctcgtgttgaagatattagcaagtgtttaggtgttggattagataatgatgttcgcatggttggaatttggggtatgggtggaatcggcaaaacaacagttgccaaagccatctataacaaattttatcatgactttgacagtagaagtttcctagcagatgttagggaaacagcaaaggacctaagtggtatgatagctctgcaagaaagacttctttctgatgtcttaaaaccagccaagataagggtgggtgatgtttccagaggcatcaatgtgatcagagaacgacttagaagcaaaaaggtacttgtcatcgttgacaatgtcgatcatctggaacaattgaatgcattggcaataagccgtgattcctttggtctgggaagtataattattataacaacaagagatcgacatttgctagagctactgaaagtggataaaatacatctagctcaagaaatgaatgaagaagaagctcttgagctcttcagttggcatgcctttcaaaattattctcctaaggaagactatatcgaactctcaagaagagtggttacttactgtggaggtttaccgctggctcttgaagttttagggtctttcctctttggaaggagcataggagattggaatagcacactgaagaaattggaaaaatttcctgatgacaaaattcagtcaaagctccgaataagctttgatgcacttgaggagtggcagaggcacatattcctccacatatgttgtttcttcattggaatggacaaaaaccatgtcataaaaatactggaaggctgtggtcttgctgcaggtgtaggcattagtgtcctctctgaacgctgcctcgtaactgttaatgaaaaagacaaggtaatgatgcatgatttgcttcgagacatgggcagagaaattgttcgtgaagaatccccgaaccaccctgaaagacgtagtagattgtggcgtgaggaagatgtaatagatgttttgagatatgaatctggaactgaagagactgaaggactcgctctaaatttgcaaggatataacaacaggaatttcagtacagaagcatttagaaacatgaggggactgaaattgctccaactcaagtatgtacgactcaatggagactgcaacaagcttcccaaaaagttaagatggctctgcttgcgaggatcctctctcttcactcgaaataagtttcgggatgcaatttacctggtttctatggacctgcggtacagcggatattttccttctgatatattatggcggttttggaatttggagatacttaatctcagtcattcacccctcccaagacaatcaccacacttttcacaagtcccaaatctacagtatttgatccttaaatactgtgtggatttgctagagattgacaggtccattggacatctttctcgacttgtccttctaaatcttaaaggctgcactaagctcaaggaccttccggaggatttttatatgttgccttctgttaggactcttgttctttctggctgttcgagatttgagaaattgagcaagcatataggaaaaatggattctttgggaactcttgttataagtggcacagccataagtgaagtgccatcctcgatagatgaattgcaatacctggacttttcatctcgacaaggcctgagtggactaaaacgatttacccaggatgacacatcacgacctaggcagcaactgcagaagccaatgaagagtagcttatgggaagcggcttatccagctcagcgtctctttgaaaaattacactctcatccgtttctctttgtgatatcctgcgcgacggatctcatggatgagaacgctcaaacggtgactaatcaccacgccattcaaatctcaatgagtctgaccgagtctccagtacactcttctagcagtgatgactttgccgggttgcttgagagagaactaaagtccagttcttcagagtcatcaccagaggaagaagatgaagatgataaaaaagctgatggtgatggtgacggcagtgatgttgaaagtgagagcaggataaaaaggcgtaagggagataatttggaaagcgtagaggaaactaatgggtcgacttcacaaggacttccagaacaaaattcaggatattga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

922

Amino Acids

104.82

Weight (kDa)

6.06

Isoelectric Point (pI)

52.66

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000055)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G36930 AT5G36930 AT5G36930 AT5G36930
fragaria_vesca FvH4_3g45361 FvH4_3g45380 FvH4_5g21061 FvH4_5g21062 FvH4_5g21063 FvH4_5g32050 FvH4_5g32970 FvH4_5g32990 FvH4_5g34190 FvH4_5g34210 FvH4_5g34210 FvH4_5g34210 FvH4_5g34210 FvH4_5g34210 FvH4_5g34210 FvH4_5g34211 FvH4_5g34240 FvH4_5g34250
malus_domestica MD00G1011700.v1.1 MD00G1026100.v1.1 MD00G1026500.v1.1 MD00G1027000.v1.1 MD00G1027100.v1.1 MD00G1027700.v1.1 MD00G1027800.v1.1 MD00G1028000.v1.1 MD00G1220000.v1.1 MD02G1024400.v1.1 MD02G1024800.v1.1 MD02G1026000.v1.1 MD02G1026100.v1.1 MD02G1031800.v1.1 MD02G1038500.v1.1 MD02G1038700.v1.1 MD02G1039200.v1.1 MD02G1040600.v1.1 MD02G1040900.v1.1 MD02G1041200.v1.1 MD02G1041300.v1.1 MD02G1041700.v1.1 MD02G1042000.v1.1 MD02G1043800.v1.1 MD02G1043900.v1.1 MD02G1044300.v1.1 MD02G1045200.v1.1 MD02G1045300.v1.1 MD02G1051200.v1.1 MD02G1052200.v1.1 MD02G1052800.v1.1 MD02G1053100.v1.1 MD02G1053600.v1.1 MD02G1055500.v1.1 MD02G1063300.v1.1 MD02G1063400.v1.1 MD02G1063800.v1.1 MD02G1306500.v1.1 MD02G1306600.v1.1 MD02G1306700.v1.1 MD02G1306900.v1.1 MD02G1307200.v1.1 MD04G1011700.v1.1 MD05G1315400.v1.1 MD05G1316000.v1.1 MD05G1316700.v1.1 MD05G1317000.v1.1 MD05G1317300.v1.1 MD05G1317600.v1.1 MD05G1317700.v1.1 MD07G1102800.v1.1 MD07G1238200.v1.1 MD08G1175900.v1.1 MD08G1203600.v1.1 MD09G1024200.v1.1 MD09G1039600.v1.1 MD09G1044800.v1.1 MD09G1045100.v1.1 MD10G1038700.v1.1 MD10G1039000.v1.1 MD10G1039200.v1.1 MD10G1040000.v1.1 MD10G1040200.v1.1 MD10G1040600.v1.1 MD10G1040800.v1.1 MD10G1297300.v1.1 MD12G1247500.v1.1 MD12G1247900.v1.1 MD12G1248300.v1.1 MD12G1248800.v1.1 MD15G1103700.v1.1 MD15G1103800.v1.1 MD15G1104000.v1.1 MD15G1179600.v1.1 MD15G1182500.v1.1 MD16G1068200.v1.1 MD16G1068300.v1.1 MD17G1024600.v1.1 MD17G1024700.v1.1 MD17G1025000.v1.1 MD17G1025900.v1.1
prunus_persica Prupe.1G539800_v2.0.a1 Prupe.1G550800_v2.0.a1 Prupe.1G550800_v2.0.a1 Prupe.1G550800_v2.0.a1 Prupe.1G550800_v2.0.a1 Prupe.1G550800_v2.0.a1 Prupe.1G550800_v2.0.a1 Prupe.1G557100_v2.0.a1 Prupe.1G557200_v2.0.a1 Prupe.1G557200_v2.0.a1 Prupe.1G557300_v2.0.a1
pyrus_communis pycom02g02080 pycom02g02090 pycom02g02110 pycom02g03220 pycom02g03260 pycom02g03370 pycom02g03390 pycom02g03410 pycom02g03420 pycom02g03450 pycom02g03500 pycom02g03730 pycom02g03770 pycom02g04400 pycom02g04410 pycom02g04430 pycom02g04450 pycom02g04460 pycom02g04490 pycom02g05200 pycom02g25710 pycom02g25750 pycom02g25790 pycom02g25810 pycom02g25830 pycom04g00920 pycom04g00930 pycom04g00940 pycom05g29300 pycom05g29310 pycom05g29380 pycom05g29410 pycom07g06310 pycom07g21420 pycom08g15070 pycom08g17540 pycom08g17560 pycom08g17570 pycom10g02670 pycom10g02680 pycom10g02700 pycom10g02770 pycom10g02790 pycom10g02800 pycom10g02810 pycom10g25020 pycom10g25030 pycom10g25050 pycom10g25080 pycom111g01990 pycom111g02000 pycom111g02030 pycom111g02040 pycom111g02050 pycom111g02060 pycom12g22710 pycom12g22720 pycom12g22730 pycom12g22740 pycom12g22750 pycom12g22770 pycom12g22780 pycom12g22790 pycom12g22800 pycom12g22810 pycom12g22830 pycom12g22840 pycom12g22860 pycom15g09440 pycom15g09480 pycom15g16120 pycom15g32380 pycom16g05970 pycom17g02100
rosa_chinensis RchiOBHm_Chr2g0144661 RchiOBHm_Chr4g0407491 RchiOBHm_Chr4g0407501 RchiOBHm_Chr4g0412381 RchiOBHm_Chr7g0208191 RchiOBHm_Chr7g0208201 RchiOBHm_Chr7g0208211 RchiOBHm_Chr7g0208221 RchiOBHm_Chr7g0228071 RchiOBHm_Chr7g0228121 RchiOBHm_Chr7g0230971 RchiOBHm_Chr7g0230981 RchiOBHm_Chr7g0233901 RchiOBHm_Chr7g0233951 RchiOBHm_Chr7g0233961 RchiOBHm_Chr7g0233991 RchiOBHm_Chr7g0234021 RchiOBHm_Chr7g0234151 RchiOBHm_Chr7g0234161 RchiOBHm_Chr7g0234241 RchiOBHm_Chr7g0234501 RchiOBHm_Chr7g0234641 RchiOBHm_Chr7g0234771 RchiOBHm_Chr7g0234781 RchiOBHm_Chr7g0234991 RchiOBHm_Chr7g0241291 RchiOBHm_Chr7g0241301
rosa_laevigata RLG00000001186 RLG00000001187 RLG00000001212 RLG00000001226 RLG00000001233 RLG00000001590 RLG00000003242 RLG00000003243 RLG00000003249 RLG00000008688 RLG00000008690
rosa_multiflora Rmu_co7966574.1_g000001 Rmu_co8120830.1_g000001 Rmu_co8142226.1_g000001 Rmu_co8293083.1_g000001 Rmu_co8351227.1_g000001 Rmu_co8380159.1_g000001 Rmu_co8456505.1_g000001 Rmu_sc0000676.1_g000025 Rmu_sc0000706.1_g000018 Rmu_sc0000706.1_g000040 Rmu_sc0000740.1_g000029 Rmu_sc0000740.1_g000032 Rmu_sc0000740.1_g000037 Rmu_sc0000740.1_g000040 Rmu_sc0000762.1_g000003 Rmu_sc0000762.1_g000019 Rmu_sc0000762.1_g000034 Rmu_sc0000762.1_g000069 Rmu_sc0000892.1_g000001 Rmu_sc0000945.1_g000040 Rmu_sc0001913.1_g000037 Rmu_sc0001913.1_g000041 Rmu_sc0001913.1_g000049 Rmu_sc0002775.1_g000017 Rmu_sc0002775.1_g000018 Rmu_sc0002775.1_g000020 Rmu_sc0002775.1_g000021 Rmu_sc0002775.1_g000022 Rmu_sc0002775.1_g000025 Rmu_sc0002775.1_g000026 Rmu_sc0002775.1_g000028 Rmu_sc0002775.1_g000030 Rmu_sc0004858.1_g000002 Rmu_sc0004864.1_g000022 Rmu_sc0005038.1_g000023 Rmu_sc0005840.1_g000014 Rmu_sc0006724.1_g000003 Rmu_sc0006824.1_g000005 Rmu_sc0007869.1_g000003 Rmu_sc0007869.1_g000005 Rmu_sc0007869.1_g000008 Rmu_sc0008646.1_g000007 Rmu_sc0009637.1_g000009 Rmu_sc0010500.1_g000002 Rmu_sc0010500.1_g000012 Rmu_sc0012403.1_g000025 Rmu_sc0013768.1_g000025 Rmu_sc0015625.1_g000001 Rmu_sc0019715.1_g000001 Rmu_sc0021133.1_g000002 Rmu_sc0021904.1_g000001 Rmu_sc0021904.1_g000003 Rmu_sc0021904.1_g000004 Rmu_sc0022054.1_g000001 Rmu_sc0026025.1_g000001 Rmu_sc0036828.1_g000002 Rmu_sc0041808.1_g000001 Rmu_ssc0000012.1_g000001
rosa_roxburghii Rroxscaffold_3G00226370 Rroxscaffold_3G00226860 Rroxscaffold_3G00226900 Rroxscaffold_3G00226930 Rroxscaffold_3G00226940 Rroxscaffold_3G00226970 Rroxscaffold_3G00226980 Rroxscaffold_3G00231530 Rroxscaffold_3G00237090 Rroxscaffold_3G00250760 Rroxscaffold_3G00250800
rosa_rugosa Rorug04G0082300 Rorug07G0101800 Rorug07G0102000 Rorug07G0102100 Rorug07G0221200 Rorug07G0245700 Rorug07G0245700 Rorug07G0245700 Rorug07G0263900 Rorug07G0264000 Rorug07G0264000 Rorug07G0264500 Rorug07G0282900 Rorug07G0282900 Rorug07G0283100 Rorug07G0283200.1 Rorug07G0283300 Rorug07G0283900 Rorug07G0284000 Rorug07G0284100 Rorug07G0284300 Rorug07G0284400 Rorug07G0284500 Rorug07G0285000 Rorug07G0285200 Rorug07G0285300 Rorug07G0285500 Rorug07G0286800 Rorug07G0286900 Rorug07G0287100 Rorug07G0287200
rosa_samantha Rh7AG236100 Rh7AG236200 Rh7AG236300 Rh7AG440000 Rh7AG440100 Rh7BG411900 Rh7BG412000 Rh7BG412600 Rh7CG251300 Rh7CG417700 Rh7CG460100 Rh7DG242100 Rh7DG242200 Rh7DG242300 Rh7DG429300
rosa_wichuraiana Rw0G005070 Rw0G010780 Rw0G012530 Rw7G020110 Rw7G020160 Rw7G020170 Rw7G033210 Rw7G033230 Rw7G033250 Rw7G034540 Rw7G035150 Rw7G036190 Rw7G036210 Rw7G036260 Rw7G036270 Rw7G036290 Rw7G036300 Rw7G036310 Rw7G036350 Rw7G036360 Rw7G036440 Rw7G036510 Rw7G036530 Rw7G036570 Rw7G036590 Rw7G036730

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1019, 2210
AarI CACCTGC 1 cut(s) 1424
Acc36I ACCTGC 2 cut(s) 1424, 1866
AccBSI CCGCTC 1 cut(s) 488
AccI GTMKAC 1 cut(s) 2729
AccII CGCG 2 cut(s) 278, 2444
AccIII TCCGGA 1 cut(s) 2095
AciI CCGC 9 cut(s) 212, 278, 488, 491, 1199, 1861, 1869, 1897, 2375
AclWI GGATC 6 cut(s) 254, 365, 1794, 1807, 1984, 2457
AcsI RAATTY 9 cut(s) 589, 684, 740, 1280, 1296, 1654, 1678, 1906, 2755
AcuI CTGAAG 6 cut(s) 82, 1096, 1286, 1650, 1659, 2574
AfaI GTAC 6 cut(s) 438, 927, 1687, 1740, 1865, 2529
AfiI CCNNNNNNNGG 4 cut(s) 142, 165, 864, 2098
AflIII ACRYGT 2 cut(s) 84, 379
AgeI ACCGGT 1 cut(s) 395
AgsI TTSAA 8 cut(s) 589, 629, 967, 1130, 1211, 2402, 2498, 2663
AhlI ACTAGT 1 cut(s) 148
AjiI CACGTC 1 cut(s) 85
AjnI CCWGG 3 cut(s) 1842, 2262, 2310
Alw21I GWGCWC 1 cut(s) 1110
Alw26I GTCTC 7 cut(s) 299, 624, 1512, 1628, 1746, 2399, 2526
AlwI GGATC 6 cut(s) 254, 365, 1794, 1807, 1984, 2457
AlwNI CAGNNNCTG 1 cut(s) 2341
Ama87I CYCGRG 1 cut(s) 296
Aor13HI TCCGGA 1 cut(s) 2095
AoxI GGCC 3 cut(s) 53, 120, 2284
ApeKI GCWGC 5 cut(s) 473, 1430, 1464, 2073, 2335
ApoI RAATTY 9 cut(s) 589, 684, 740, 1280, 1296, 1654, 1678, 1906, 2755
ArsI GACNNNNNNTTYG 2 cut(s) 1383, 1415
AsiGI ACCGGT 1 cut(s) 395
Asp700I GAANNNNTTC 4 cut(s) 122, 831, 1281, 1536
AspA2I CCTAGG 1 cut(s) 2330
AspLEI GCGC 1 cut(s) 2444
AspS9I GGNCC 4 cut(s) 799, 1855, 2028, 2089
AsuC2I CCSGG 1 cut(s) 2557
AsuHPI GGTGA 9 cut(s) 116, 884, 1926, 1944, 2477, 2488, 2592, 2651, 2657
AvaI CYCGRG 1 cut(s) 296
AvaII GGWCC 4 cut(s) 799, 1855, 2028, 2089
AvrII CCTAGG 1 cut(s) 2330
AxyI CCTNAGG 1 cut(s) 1143
BamHI GGATCC 1 cut(s) 1799
BanII GRGCYC 1 cut(s) 1110
BarI GAAGNNNNNNTAC 4 cut(s) 420, 452, 1162, 1194
BauI CACGAG 1 cut(s) 621
BbsI GAAGAC 3 cut(s) 114, 253, 1155
Bbv12I GWGCWC 1 cut(s) 1110
BbvI GCAGC 5 cut(s) 460, 1417, 1451, 2060, 2347
BccI CCATC 7 cut(s) 40, 52, 737, 1776, 2245, 2630, 2636
BceAI ACGGC 3 cut(s) 159, 969, 2665
BciT130I CCWGG 3 cut(s) 1844, 2264, 2312
BclI TGATCA 1 cut(s) 897
BcnI CCSGG 1 cut(s) 2557
BcoDI GTCTC 7 cut(s) 299, 624, 1512, 1628, 1746, 2399, 2526
BcuI ACTAGT 1 cut(s) 148
BfaI CTAG 7 cut(s) 149, 773, 1043, 1073, 2015, 2331, 2538
BfmI CTRYAG 3 cut(s) 1431, 1979, 2342
BfuAI ACCTGC 2 cut(s) 1424, 1866
BisI GCNGC 7 cut(s) 474, 489, 1431, 1465, 2074, 2336, 2376
BlnI CCTAGG 1 cut(s) 2330
BlpI GCTNAGC 1 cut(s) 2388
BlsI GCNGC 7 cut(s) 475, 490, 1432, 1466, 2075, 2337, 2377
Bme1390I CCNGG 4 cut(s) 1844, 2264, 2312, 2557
Bme18I GGWCC 4 cut(s) 799, 1855, 2028, 2089
BmeT110I CYCGRG 1 cut(s) 296
BmgBI CACGTC 1 cut(s) 85
BmgT120I GGNCC 4 cut(s) 799, 1855, 2028, 2089
BmiI GGNNCC 1 cut(s) 1801
BmrFI CCNGG 4 cut(s) 1844, 2264, 2312, 2557
BmrI ACTGGG 1 cut(s) 599
BmsI GCATC 6 cut(s) 17, 169, 897, 1315, 1491, 1822
BmuI ACTGGG 1 cut(s) 599
BpiI GAAGAC 3 cut(s) 114, 253, 1155
BplI GAGNNNNNCTC 2 cut(s) 460, 492
BpmI CTGGAG 1 cut(s) 2508
Bpu1102I GCTNAGC 1 cut(s) 2388
BpuEI CTTGAG 9 cut(s) 34, 112, 1062, 1124, 1150, 1352, 1715, 2069, 2585
BpuMI CCSGG 1 cut(s) 2557
BsaBI GATNNNNATC 1 cut(s) 1618
BsaJI CCNNGG 4 cut(s) 183, 235, 2310, 2330
BsaWI WCCGGW 3 cut(s) 58, 395, 2095
BsaXI ACNNNNNCTCC 2 cut(s) 2506, 2536
Bsc4I CCNNNNNNNGG 4 cut(s) 142, 165, 864, 2098
Bse118I RCCGGY 1 cut(s) 395
Bse1I ACTGG 4 cut(s) 605, 1416, 2525, 2583
Bse21I CCTNAGG 1 cut(s) 1143
Bse8I GATNNNNATC 1 cut(s) 1618
BseAI TCCGGA 1 cut(s) 2095
BseBI CCWGG 3 cut(s) 1844, 2264, 2312
BseDI CCNNGG 4 cut(s) 183, 235, 2310, 2330
BseGI GGATG 8 cut(s) 17, 44, 184, 1837, 2237, 2320, 2416, 2464
BseJI GATNNNNATC 1 cut(s) 1618
BseLI CCNNNNNNNGG 4 cut(s) 142, 165, 864, 2098
BseMII CTCAG 4 cut(s) 279, 1939, 2279, 2402
BseNI ACTGG 4 cut(s) 605, 1416, 2525, 2583
BseRI GAGGAG 3 cut(s) 314, 498, 1349
BseXI GCAGC 5 cut(s) 460, 1417, 1451, 2060, 2347
BsgI GTGCAG 2 cut(s) 329, 2059
Bsh1236I CGCG 2 cut(s) 278, 2444
BshFI GGCC 3 cut(s) 55, 122, 2286
BshTI ACCGGT 1 cut(s) 395
BsiHKAI GWGCWC 1 cut(s) 1110
BsiHKCI CYCGRG 1 cut(s) 296
BsiSI CCGG 5 cut(s) 59, 159, 396, 2096, 2556
BslFI GGGAC 4 cut(s) 52, 201, 1725, 1955
BslI CCNNNNNNNGG 4 cut(s) 142, 165, 864, 2098
BsmAI GTCTC 7 cut(s) 299, 624, 1512, 1628, 1746, 2399, 2526
BsmBI CGTCTC 1 cut(s) 2399
BsmFI GGGAC 4 cut(s) 52, 201, 1725, 1955
BsmI GAATGC 1 cut(s) 973
BsnI GGCC 3 cut(s) 55, 122, 2286
BsoBI CYCGRG 1 cut(s) 296
Bsp1286I GDGCHC 1 cut(s) 1110
Bsp13I TCCGGA 1 cut(s) 2095
Bsp143I GATC 9 cut(s) 246, 312, 370, 897, 949, 1030, 1799, 1989, 2449
Bsp1720I GCTNAGC 1 cut(s) 2388
BspACI CCGC 9 cut(s) 212, 278, 488, 491, 1199, 1861, 1869, 1897, 2375
BspANI GGCC 3 cut(s) 55, 122, 2286
BspCNI CTCAG 4 cut(s) 278, 1938, 2280, 2401
BspEI TCCGGA 1 cut(s) 2095
BspFNI CGCG 2 cut(s) 278, 2444
BspHI TCATGA 1 cut(s) 748
BspLI GGNNCC 1 cut(s) 1801
BspMAI CTGCAG 2 cut(s) 1435, 2346
BspMI ACCTGC 2 cut(s) 1424, 1866
BspPI GGATC 6 cut(s) 254, 365, 1794, 1807, 1984, 2457
BspQI GCTCTTC 1 cut(s) 1115
BsrBI CCGCTC 1 cut(s) 488
BsrFI RCCGGY 1 cut(s) 395
BsrI ACTGG 4 cut(s) 605, 1416, 2525, 2583
BssAI RCCGGY 1 cut(s) 395
BssECI CCNNGG 4 cut(s) 183, 235, 2310, 2330
BssMI GATC 9 cut(s) 246, 312, 370, 897, 949, 1030, 1799, 1989, 2449
BssSI CACGAG 1 cut(s) 621
BssT1I CCWWGG 2 cut(s) 235, 2330
Bst2BI CACGAG 1 cut(s) 621
Bst2UI CCWGG 3 cut(s) 1844, 2264, 2312
Bst4CI ACNGT 7 cut(s) 718, 761, 1187, 1477, 1983, 2003, 2476
Bst6I CTCTTC 6 cut(s) 1115, 1165, 1626, 1814, 2351, 2539
BstAPI GCANNNNNTGC 2 cut(s) 1510, 2341
BstC8I GCNNGC 5 cut(s) 502, 1122, 1203, 1793, 2171
BstENI CCTNNNNNAGG 1 cut(s) 2096
BstF5I GGATG 8 cut(s) 17, 44, 184, 1837, 2237, 2320, 2416, 2464
BstFNI CGCG 2 cut(s) 278, 2444
BstHHI GCGC 1 cut(s) 2444
BstKTI GATC 9 cut(s) 249, 315, 373, 900, 952, 1033, 1802, 1992, 2452
BstMAI GTCTC 7 cut(s) 299, 624, 1512, 1628, 1746, 2399, 2526
BstMBI GATC 9 cut(s) 246, 312, 370, 897, 949, 1030, 1799, 1989, 2449
BstMWI GCNNNNNNNGC 5 cut(s) 497, 1510, 1792, 2341, 2384
BstNI CCWGG 3 cut(s) 1844, 2264, 2312
BstNSI RCATGY 2 cut(s) 383, 1124
BstSCI CCNGG 4 cut(s) 1842, 2262, 2310, 2555
BstSFI CTRYAG 3 cut(s) 1431, 1979, 2342
BstUI CGCG 2 cut(s) 278, 2444
BstV1I GCAGC 5 cut(s) 460, 1417, 1451, 2060, 2347
BstV2I GAAGAC 3 cut(s) 114, 253, 1155
BstX2I RGATCY 3 cut(s) 246, 1799, 2449
BstYI RGATCY 3 cut(s) 246, 1799, 2449
Bsu36I CCTNAGG 1 cut(s) 1143
BsuRI GGCC 3 cut(s) 55, 122, 2286
BtgZI GCGATG 1 cut(s) 9
BtrI CACGTC 1 cut(s) 85
BtsCI GGATG 8 cut(s) 17, 44, 184, 1837, 2237, 2320, 2416, 2464
BtsI GCAGTG 2 cut(s) 2548, 2659
BtsIMutI CAGTG 3 cut(s) 1262, 2548, 2659
BveI ACCTGC 2 cut(s) 1424, 1866
Cac8I GCNNGC 5 cut(s) 502, 1122, 1203, 1793, 2171
CaiI CAGNNNCTG 1 cut(s) 2341
CciI TCATGA 1 cut(s) 748
CfoI GCGC 1 cut(s) 2444
Cfr10I RCCGGY 1 cut(s) 395
Cfr13I GGNCC 4 cut(s) 799, 1855, 2028, 2089
CseI GACGC 1 cut(s) 2381
CsiI ACCWGGT 1 cut(s) 1842
Csp6I GTAC 6 cut(s) 437, 926, 1686, 1739, 1864, 2528
CspAI ACCGGT 1 cut(s) 395
CspCI CAANNNNNGTGG 4 cut(s) 1155, 1190, 1349, 1384
CviQI GTAC 6 cut(s) 437, 926, 1686, 1739, 1864, 2528
DpnI GATC 9 cut(s) 248, 314, 372, 899, 951, 1032, 1801, 1991, 2451
DpnII GATC 9 cut(s) 246, 312, 370, 897, 949, 1030, 1799, 1989, 2449
Eam1104I CTCTTC 6 cut(s) 1115, 1165, 1626, 1814, 2351, 2539
EarI CTCTTC 6 cut(s) 1115, 1165, 1626, 1814, 2351, 2539
EciI GGCGGA 1 cut(s) 506
Ecl136II GAGCTC 1 cut(s) 1108
Eco130I CCWWGG 2 cut(s) 235, 2330
Eco147I AGGCCT 2 cut(s) 122, 2286
Eco24I GRGCYC 1 cut(s) 1110
Eco32I GATATC 1 cut(s) 2436
Eco47I GGWCC 4 cut(s) 799, 1855, 2028, 2089
Eco53kI GAGCTC 1 cut(s) 1108
Eco57I CTGAAG 6 cut(s) 82, 1096, 1286, 1650, 1659, 2574
Eco81I CCTNAGG 1 cut(s) 1143
Eco88I CYCGRG 1 cut(s) 296
EcoICRI GAGCTC 1 cut(s) 1108
EcoNI CCTNNNNNAGG 1 cut(s) 2096
EcoO109I RGGNCCY 2 cut(s) 799, 2089
EcoRII CCWGG 3 cut(s) 1842, 2262, 2310
EcoRV GATATC 1 cut(s) 2436
EcoT14I CCWWGG 2 cut(s) 235, 2330
EcoT22I ATGCAT 2 cut(s) 973, 1506
EcoT38I GRGCYC 1 cut(s) 1110
ErhI CCWWGG 2 cut(s) 235, 2330
Esp3I CGTCTC 1 cut(s) 2399
FalI AAGNNNNNCTT 6 cut(s) 816, 848, 1086, 1118, 2348, 2380
FaqI GGGAC 4 cut(s) 52, 201, 1725, 1955
FauNDI CATATG 1 cut(s) 1364
FbaI TGATCA 1 cut(s) 897
FblI GTMKAC 1 cut(s) 2729
Fnu4HI GCNGC 7 cut(s) 474, 489, 1431, 1465, 2074, 2336, 2376
FokI GGATG 8 cut(s) 4, 31, 191, 1844, 2224, 2327, 2403, 2471
FriOI GRGCYC 1 cut(s) 1110
Fsp4HI GCNGC 7 cut(s) 474, 489, 1431, 1465, 2074, 2336, 2376
FspBI CTAG 7 cut(s) 149, 773, 1043, 1073, 2015, 2331, 2538
GlaI GCGC 1 cut(s) 2443
GluI GCNGC 7 cut(s) 474, 489, 1431, 1465, 2074, 2336, 2376
GsuI CTGGAG 1 cut(s) 2508
HaeIII GGCC 3 cut(s) 55, 122, 2286
HapII CCGG 5 cut(s) 59, 159, 396, 2096, 2556
HgaI GACGC 1 cut(s) 2381
HhaI GCGC 1 cut(s) 2444
Hin6I GCGC 1 cut(s) 2442
HinP1I GCGC 1 cut(s) 2442
HincII GTYRAC 3 cut(s) 553, 940, 2730
HindII GTYRAC 3 cut(s) 553, 940, 2730
HindIII AAGCTT 2 cut(s) 1318, 1763
HpaII CCGG 5 cut(s) 59, 159, 396, 2096, 2556
HphI GGTGA 9 cut(s) 116, 884, 1926, 1944, 2477, 2488, 2592, 2651, 2657
Hpy166II GTNNAC 6 cut(s) 553, 940, 1196, 2294, 2530, 2730
Hpy8I GTNNAC 6 cut(s) 553, 940, 1196, 2294, 2530, 2730
Hpy99I CGWCG 2 cut(s) 86, 2449
HpyCH4III ACNGT 7 cut(s) 718, 761, 1187, 1477, 1983, 2003, 2476
HpyCH4IV ACGT 3 cut(s) 84, 170, 1570
HpyF10VI GCNNNNNNNGC 5 cut(s) 497, 1510, 1792, 2341, 2384
HpySE526I ACGT 3 cut(s) 84, 170, 1570
HspAI GCGC 1 cut(s) 2442
Kpn2I TCCGGA 1 cut(s) 2095
Ksp22I TGATCA 1 cut(s) 897
Kzo9I GATC 9 cut(s) 246, 312, 370, 897, 949, 1030, 1799, 1989, 2449
LguI GCTCTTC 1 cut(s) 1115
LmnI GCTCC 5 cut(s) 301, 485, 1239, 1314, 1728
Lsp1109I GCAGC 5 cut(s) 460, 1417, 1451, 2060, 2347
LweI GCATC 6 cut(s) 17, 169, 897, 1315, 1491, 1822
MabI ACCWGGT 1 cut(s) 1842
MaeI CTAG 7 cut(s) 149, 773, 1043, 1073, 2015, 2331, 2538
MaeII ACGT 3 cut(s) 84, 170, 1570
MaeIII GTNAC 4 cut(s) 1177, 1471, 2476, 2645
MalI GATC 9 cut(s) 248, 314, 372, 899, 951, 1032, 1801, 1991, 2451
MbiI CCGCTC 1 cut(s) 488
MboI GATC 9 cut(s) 246, 312, 370, 897, 949, 1030, 1799, 1989, 2449
MfeI CAATTG 1 cut(s) 962
MflI RGATCY 3 cut(s) 246, 1799, 2449
MhlI GDGCHC 1 cut(s) 1110
MlyI GAGTC 8 cut(s) 171, 626, 1638, 1737, 2122, 2518, 2528, 2603
MmeI TCCRAC 4 cut(s) 589, 634, 661, 1750
Mph1103I ATGCAT 2 cut(s) 973, 1506
MroI TCCGGA 1 cut(s) 2095
MroXI GAANNNNTTC 4 cut(s) 122, 831, 1281, 1536
MseI TTAA 7 cut(s) 69, 848, 1479, 1778, 1920, 1995, 2067
MslI CAYNNNNRTG 2 cut(s) 893, 1382
MspA1I CMGCKG 3 cut(s) 571, 1201, 1869
MspI CCGG 5 cut(s) 59, 159, 396, 2096, 2556
MspR9I CCNGG 4 cut(s) 1844, 2264, 2312, 2557
MunI CAATTG 1 cut(s) 962
Mva1269I GAATGC 1 cut(s) 973
MvaI CCWGG 3 cut(s) 1844, 2264, 2312
MvnI CGCG 2 cut(s) 278, 2444
MwoI GCNNNNNNNGC 5 cut(s) 497, 1510, 1792, 2341, 2384
NciI CCSGG 1 cut(s) 2557
NdeI CATATG 1 cut(s) 1364
NdeII GATC 9 cut(s) 246, 312, 370, 897, 949, 1030, 1799, 1989, 2449
NlaIV GGNNCC 1 cut(s) 1801
NmeAIII GCCGAG 1 cut(s) 208
NmuCI GTSAC 2 cut(s) 2476, 2645
NsiI ATGCAT 2 cut(s) 973, 1506
NspI RCATGY 2 cut(s) 383, 1124
PaeI GCATGC 1 cut(s) 1124
PaeR7I CTCGAG 1 cut(s) 296
PagI TCATGA 1 cut(s) 748
PaqCI CACCTGC 1 cut(s) 1424
PceI AGGCCT 2 cut(s) 122, 2286
PciI ACATGT 1 cut(s) 379
PciSI GCTCTTC 1 cut(s) 1115
PctI GAATGC 1 cut(s) 973
PdmI GAANNNNTTC 4 cut(s) 122, 831, 1281, 1536
PfeI GAWTC 6 cut(s) 281, 702, 989, 1547, 1619, 2189
PflFI GACNNNGTC 2 cut(s) 944, 2518
PinAI ACCGGT 1 cut(s) 395
PkrI GCNGC 7 cut(s) 475, 490, 1432, 1466, 2075, 2337, 2377
PleI GAGTC 8 cut(s) 170, 625, 1638, 1737, 2122, 2517, 2527, 2602
PpsI GAGTC 8 cut(s) 170, 625, 1638, 1737, 2122, 2517, 2527, 2602
PpuMI RGGWCCY 2 cut(s) 799, 2089
PscI ACATGT 1 cut(s) 379
PsiI TTATAA 2 cut(s) 1019, 2210
Psp124BI GAGCTC 1 cut(s) 1110
Psp5II RGGWCCY 2 cut(s) 799, 2089
Psp6I CCWGG 3 cut(s) 1842, 2262, 2310
PspGI CCWGG 3 cut(s) 1842, 2262, 2310
PspN4I GGNNCC 1 cut(s) 1801
PspPI GGNCC 4 cut(s) 799, 1855, 2028, 2089
PspPPI RGGWCCY 2 cut(s) 799, 2089
PstI CTGCAG 2 cut(s) 1435, 2346
PstNI CAGNNNCTG 1 cut(s) 2341
PsuI RGATCY 3 cut(s) 246, 1799, 2449
PsyI GACNNNGTC 2 cut(s) 944, 2518
PvuII CAGCTG 1 cut(s) 571
RsaI GTAC 6 cut(s) 438, 927, 1687, 1740, 1865, 2529
RsaNI GTAC 6 cut(s) 437, 926, 1686, 1739, 1864, 2528
RseI CAYNNNNRTG 2 cut(s) 893, 1382
SacI GAGCTC 1 cut(s) 1110
SalI GTCGAC 1 cut(s) 2728
SapI GCTCTTC 1 cut(s) 1115
SaqAI TTAA 7 cut(s) 69, 848, 1479, 1778, 1920, 1995, 2067
SatI GCNGC 7 cut(s) 474, 489, 1431, 1465, 2074, 2336, 2376
Sau3AI GATC 9 cut(s) 246, 312, 370, 897, 949, 1030, 1799, 1989, 2449
Sau96I GGNCC 4 cut(s) 799, 1855, 2028, 2089
SchI GAGTC 8 cut(s) 171, 626, 1638, 1737, 2122, 2518, 2528, 2603
ScrFI CCNGG 4 cut(s) 1844, 2264, 2312, 2557
SduI GDGCHC 1 cut(s) 1110
SexAI ACCWGGT 1 cut(s) 1842
SfaNI GCATC 6 cut(s) 17, 169, 897, 1315, 1491, 1822
SfcI CTRYAG 3 cut(s) 1431, 1979, 2342
Sfr274I CTCGAG 1 cut(s) 296
SinI GGWCC 4 cut(s) 799, 1855, 2028, 2089
SlaI CTCGAG 1 cut(s) 296
SmiMI CAYNNNNRTG 2 cut(s) 893, 1382
SpeI ACTAGT 1 cut(s) 148
SphI GCATGC 1 cut(s) 1124
SseBI AGGCCT 2 cut(s) 122, 2286
SsiI CCGC 9 cut(s) 212, 278, 488, 491, 1199, 1861, 1869, 1897, 2375
SspI AATATT 1 cut(s) 357
SspMI CTAG 7 cut(s) 149, 773, 1043, 1073, 2015, 2331, 2538
SstI GAGCTC 1 cut(s) 1110
StuI AGGCCT 2 cut(s) 122, 2286
StyD4I CCNGG 4 cut(s) 1842, 2262, 2310, 2555
StyI CCWWGG 2 cut(s) 235, 2330
TaaI ACNGT 7 cut(s) 718, 761, 1187, 1477, 1983, 2003, 2476
TaiI ACGT 3 cut(s) 87, 173, 1573
TaqII GACCGA 1 cut(s) 2531
TatI WGTACW 3 cut(s) 436, 1685, 2527
TauI GCSGC 2 cut(s) 491, 2378
TfiI GAWTC 6 cut(s) 281, 702, 989, 1547, 1619, 2189
Tru1I TTAA 7 cut(s) 69, 848, 1479, 1778, 1920, 1995, 2067
Tru9I TTAA 7 cut(s) 69, 848, 1479, 1778, 1920, 1995, 2067
TscAI CASTG 3 cut(s) 1269, 2548, 2659
TseFI GTSAC 2 cut(s) 2476, 2645
TseI GCWGC 5 cut(s) 473, 1430, 1464, 2073, 2335
Tsp45I GTSAC 2 cut(s) 2476, 2645
TspGWI ACGGA 3 cut(s) 130, 2409, 2462
TspRI CASTG 3 cut(s) 1269, 2548, 2659
Tth111I GACNNNGTC 2 cut(s) 944, 2518
VpaK11BI GGWCC 4 cut(s) 799, 1855, 2028, 2089
XagI CCTNNNNNAGG 1 cut(s) 2096
XapI RAATTY 9 cut(s) 589, 684, 740, 1280, 1296, 1654, 1678, 1906, 2755
XceI RCATGY 2 cut(s) 383, 1124
XcmI CCANNNNNNNNNTGG 1 cut(s) 865
XhoI CTCGAG 1 cut(s) 296
XmaJI CCTAGG 1 cut(s) 2330
XmiI GTMKAC 1 cut(s) 2729
XmnI GAANNNNTTC 4 cut(s) 122, 831, 1281, 1536
XspI CTAG 7 cut(s) 149, 773, 1043, 1073, 2015, 2331, 2538
Zsp2I ATGCAT 2 cut(s) 973, 1506
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.