MD02G1053100.v1.1

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0000055
Physical Location & Seq
Reverse (-)
4338781 .. 4346208
7428 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1053100.v1.1.491

Sequence Viewer

Length: 3288 bp
ATGACAACCCACGAAGCCTCCTCGTCATCCTCCTCCAAGTCAAAACTTTGGAATTACGACGTGTTCTTGAGCTTCAGCGGTGTGGACACACGCAATGGCTTCACGGGCCACCTCCATGCGGCATTAACAGAGAGGGGATACCAGGCTTATATCGATGAGGACGATCTAGAAAGAGGGGAAGAAATAAAAGAGGAACTGTTCCGGGCAATTGAAGAGGCGAGGATCTCTATCATTGTCTTCTCAAAGAGTTATGCGAACTCGAGTTGGTGTCTTGACGAGCTGGTGAAGATCATGAAGTGCAGATACAAACTGGGGCGACGTGTTTTGCCAATATTCTATCATGTTGATCCTTCACATGTTAGGAAGCAGAACGGAGATTTAGCTCAAGCATTTCAGAAGCACGAAGAGGGCATTCGTGAAGAAAAAGATGACAAGGAACGTGAAGCTAAACAAGAAAGGGTAAAGCAATGGAGAAAGGCTCTTACAGAAGCTGCAAATTTGTCTGGCCAACATCTTCAAATCATTGACAATGGGCGCGAAGCAAAGCTTATTAGAGAAATTGTTGGCAAGATCATTACGGAATGGCTTCCGGGTGCAAACGAATTAAATGTGGCCAAGCACCCGGTTGGAATCAATTCTCGCGTTCAAGATATTATCAATCATCTTTCAGGTGGTGGATCAAATGACGTTGTCATGGTTGGAATTTGGGGGATGGGTGGATTAGGTAAAACAACAGCTGCCAAAGCCATTTATAACCAAATTCATCATAAGTTCCAATTCAAAAGTTTCCTTGCCAACGTTAGTGAATATGATTTGGTTGATTTGCAAGGAAAGCTTGTTTCTGACATCTTGAAACAGACTGAGTCTAAGATAACCAGTGTTGATGGAGGTATCAGTTTGCTAAAAAATCATCTCCAACGTAGAAGCGTACTTGTCATTATTGACAATGTAGACAAAGTGGAACAACTAAATGCCATAGCTGGAAATCGTGATTGGTTTGGTCCAGGAAGTAGAATTATCATAACGACACGAGATGAATGTTTACTAAAGCAAGTGAACATGAAAGTGGACAAGACATATCCGCTTAAGGAAATGAATGAAGAAGAAGCTCTGAAGCTCTTTAGTTGGCATGCCTTTGGAAAGAGTTGGCCTAATGAAGGATATCTTGAACTCTCAAAAGAGGTTGTTTCTTACTGTGGTGGTTTGCCAATAGCCCTTGAAGTTTTAGGTTCTTCTATGATTGAAAGAACCCCAATAGAGTGGAAAAGTCAGTTGGAGAAATTGAAAAAATTTCCTGATAAAGGAATAATGAAACCGCTAAGAGTAAGCTTTGAAAGGCTAGATCCTACACAAAAGGATATATTCCTTGACATTTCTTGTTTCTTTATTGGATGGGATAAGGACTATGTCGCAAAAATATTAGATGGATGTAACTTTTTTGCAACAATAGGAATCAGTGACCTCCGTGAACGATGCCTTATAACTGTTGAAGACAACGTGTTGAATATGCATGATTTGCTTCGAGAAATGGCCAAATTAATCATTTCTGAAAAATCCCCTCGTCAGCCTGGAGAGTGGAGTAGGTTGTGGAACCGGCAAGAGGTCATTAAAGTATTGAGAGATAAATCTGGAACTGAAGAAGTTGAAGGACTTGCTCTAGGTATCCCTCCTCCTTGGCCTCCAAGGTCTAACATGCCTGGGTTCAGTACAGAAGCATTTGCCAATATGAAGAAACTGAGACTGCTCAAGCTCTACAACGTGCAGCTCAATGGAGAATACAAACATCTCCCCAAGGAGTTAATATGGTTGTGTTGGGAAAGATGCCCTTTAAAGTCCATACCAGATGACTTCTTTGATCAACCAAGACTAGTTATTTTAGAGATAACATCTAGCGACCTGGTACAAGTTTGGGAGGGCTCCAAGTCACTTGAAAACTTGAAGGTCCTTAATCTTGGAAGTTGCTCTGACTTAAAAAAATCACCAGACTTTTCAAATCTCCCAAATCTTGAAGAGTTGATATTGGAAAGGTGTAGGAGTTTGTCCGATATTCACCCCTCCATTGGTCATCTTAAAAAACTTTCTTTGGTGAACCTCACACGCTGCAAGAATCTTATTTCTCTTCCTGGGGATTTCTACAGGTCCAAATCCGTTCAGACTCTTGTTCTTGATTTTTGTTGGCAATTCAGTGAATTGCCCGAGGGTTTAGGGAAGATGATATCATTGAGAGTACTTAAAGCATCTACTACAGCCATAAGACAAGTACCGCGTTCCACAGTAAGATTGAAGAATCTCACTCATTTATCTCTCAATAATTTTGTGGGATCGAAGTTTCCTTTGCAATTTCCCGATTCGTTACACGGCTTAGACTCTTTAAGAAATTTAGATCTCTCAGTCAATTATTTTCATACCCTACCCAGCCTCAGTGGTCTTTCAAAACTTGAAAGTTTGTGGTTAAGTTATTGCTCCCAGCTTCATACAATCTATGATTTACCAACAAATTTGAAATTTCTGGATGCATCTAGTTGCTATCGTTTGGTAACAATGCCCAATTTTTCGAAAATGTCAAATATGAGGGAACTGAATGTATGTGATTCACACGAACTCACTGAGGTTCCAGACTTGGATAAGTCATTAAACTCCATGACATGGATTGATATGAGTAAGTGCACCAAACTCACGGCTGATTTAAGGAGGAACGTCCTAAAGGGATGGACTTCTTGCGGATATGGTGGCATTTTTCTCAATGGGAATTATGTTCCTGATTGGTTCGAGTTTGTCAATGATGGTAATGAAGTTAGCTTTGATATTCCCCCGGGTGATGGTCGTAGTTTTGAAGGGCTAACTCTGTTATGCTTTTACCGCTCTTATCTTGATCGGCCAAGACCTTATATGAGAGGTAAAGATGGTCATCCTCGTGCTAGTATTGATATAAATAATACCAAGCGTACTGAGTTACAGACTTGCATAGGCAAGGAAGATTGGGTTACAAAAATGCGCGATATTGTATTTGAAAGCTGTTATCTTTGGCAAGGTCAAATATCAAACGATAAGCTCAATTTGCAAAGCGGGGATAAAGTTGATATAACTTTTGAAATACCAATGAAAGGCGGTGCATTTAATTATGCGAAAACTAATGCATTTAATTATGCGAAAGCTAAGGGAATAGGGGTTAATCTAGTATGGGACAAACCTATGAAGGAAAATATGCACGATTTTGATCTTGATGGTAGGTTTTTCATCCAGAGACACAATCAAGGTGGTGATGCATCGTCATCATCACATGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

1096

Amino Acids

124.71

Weight (kDa)

7.31

Isoelectric Point (pI)

38.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 18 - 195 1.4e-49 TIR domain
TIR_2 PF13676 21 - 128 1.2e-17 TIR domain
NB-ARC PF00931 213 - 381 4.1e-19 NB-ARC domain
WHD_ROQ1 PF23282 451 - 519 3.7e-12 Disease resistance protein Roq1-like, winged-helix domain
LRR_14 PF23598 727 - 872 6e-09 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000055)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G36930 AT5G36930 AT5G36930 AT5G36930
fragaria_vesca FvH4_3g45361 FvH4_3g45380 FvH4_5g21061 FvH4_5g21062 FvH4_5g21063 FvH4_5g32050 FvH4_5g32970 FvH4_5g32990 FvH4_5g34190 FvH4_5g34210 FvH4_5g34210 FvH4_5g34210 FvH4_5g34210 FvH4_5g34210 FvH4_5g34210 FvH4_5g34211 FvH4_5g34240 FvH4_5g34250
malus_domestica MD00G1011700.v1.1 MD00G1026100.v1.1 MD00G1026500.v1.1 MD00G1027000.v1.1 MD00G1027100.v1.1 MD00G1027700.v1.1 MD00G1027800.v1.1 MD00G1028000.v1.1 MD00G1220000.v1.1 MD02G1024400.v1.1 MD02G1024800.v1.1 MD02G1026000.v1.1 MD02G1026100.v1.1 MD02G1031800.v1.1 MD02G1038500.v1.1 MD02G1038700.v1.1 MD02G1039200.v1.1 MD02G1040600.v1.1 MD02G1040900.v1.1 MD02G1041200.v1.1 MD02G1041300.v1.1 MD02G1041700.v1.1 MD02G1042000.v1.1 MD02G1043800.v1.1 MD02G1043900.v1.1 MD02G1044300.v1.1 MD02G1045200.v1.1 MD02G1045300.v1.1 MD02G1051200.v1.1 MD02G1052200.v1.1 MD02G1052800.v1.1 MD02G1053100.v1.1 MD02G1053600.v1.1 MD02G1055500.v1.1 MD02G1063300.v1.1 MD02G1063400.v1.1 MD02G1063800.v1.1 MD02G1306500.v1.1 MD02G1306600.v1.1 MD02G1306700.v1.1 MD02G1306900.v1.1 MD02G1307200.v1.1 MD04G1011700.v1.1 MD05G1315400.v1.1 MD05G1316000.v1.1 MD05G1316700.v1.1 MD05G1317000.v1.1 MD05G1317300.v1.1 MD05G1317600.v1.1 MD05G1317700.v1.1 MD07G1102800.v1.1 MD07G1238200.v1.1 MD08G1175900.v1.1 MD08G1203600.v1.1 MD09G1024200.v1.1 MD09G1039600.v1.1 MD09G1044800.v1.1 MD09G1045100.v1.1 MD10G1038700.v1.1 MD10G1039000.v1.1 MD10G1039200.v1.1 MD10G1040000.v1.1 MD10G1040200.v1.1 MD10G1040600.v1.1 MD10G1040800.v1.1 MD10G1297300.v1.1 MD12G1247500.v1.1 MD12G1247900.v1.1 MD12G1248300.v1.1 MD12G1248800.v1.1 MD15G1103700.v1.1 MD15G1103800.v1.1 MD15G1104000.v1.1 MD15G1179600.v1.1 MD15G1182500.v1.1 MD16G1068200.v1.1 MD16G1068300.v1.1 MD17G1024600.v1.1 MD17G1024700.v1.1 MD17G1025000.v1.1 MD17G1025900.v1.1
prunus_persica Prupe.1G539800_v2.0.a1 Prupe.1G550800_v2.0.a1 Prupe.1G550800_v2.0.a1 Prupe.1G550800_v2.0.a1 Prupe.1G550800_v2.0.a1 Prupe.1G550800_v2.0.a1 Prupe.1G550800_v2.0.a1 Prupe.1G557100_v2.0.a1 Prupe.1G557200_v2.0.a1 Prupe.1G557200_v2.0.a1 Prupe.1G557300_v2.0.a1
pyrus_communis pycom02g02080 pycom02g02090 pycom02g02110 pycom02g03220 pycom02g03260 pycom02g03370 pycom02g03390 pycom02g03410 pycom02g03420 pycom02g03450 pycom02g03500 pycom02g03730 pycom02g03770 pycom02g04400 pycom02g04410 pycom02g04430 pycom02g04450 pycom02g04460 pycom02g04490 pycom02g05200 pycom02g25710 pycom02g25750 pycom02g25790 pycom02g25810 pycom02g25830 pycom04g00920 pycom04g00930 pycom04g00940 pycom05g29300 pycom05g29310 pycom05g29380 pycom05g29410 pycom07g06310 pycom07g21420 pycom08g15070 pycom08g17540 pycom08g17560 pycom08g17570 pycom10g02670 pycom10g02680 pycom10g02700 pycom10g02770 pycom10g02790 pycom10g02800 pycom10g02810 pycom10g25020 pycom10g25030 pycom10g25050 pycom10g25080 pycom111g01990 pycom111g02000 pycom111g02030 pycom111g02040 pycom111g02050 pycom111g02060 pycom12g22710 pycom12g22720 pycom12g22730 pycom12g22740 pycom12g22750 pycom12g22770 pycom12g22780 pycom12g22790 pycom12g22800 pycom12g22810 pycom12g22830 pycom12g22840 pycom12g22860 pycom15g09440 pycom15g09480 pycom15g16120 pycom15g32380 pycom16g05970 pycom17g02100
rosa_chinensis RchiOBHm_Chr2g0144661 RchiOBHm_Chr4g0407491 RchiOBHm_Chr4g0407501 RchiOBHm_Chr4g0412381 RchiOBHm_Chr7g0208191 RchiOBHm_Chr7g0208201 RchiOBHm_Chr7g0208211 RchiOBHm_Chr7g0208221 RchiOBHm_Chr7g0228071 RchiOBHm_Chr7g0228121 RchiOBHm_Chr7g0230971 RchiOBHm_Chr7g0230981 RchiOBHm_Chr7g0233901 RchiOBHm_Chr7g0233951 RchiOBHm_Chr7g0233961 RchiOBHm_Chr7g0233991 RchiOBHm_Chr7g0234021 RchiOBHm_Chr7g0234151 RchiOBHm_Chr7g0234161 RchiOBHm_Chr7g0234241 RchiOBHm_Chr7g0234501 RchiOBHm_Chr7g0234641 RchiOBHm_Chr7g0234771 RchiOBHm_Chr7g0234781 RchiOBHm_Chr7g0234991 RchiOBHm_Chr7g0241291 RchiOBHm_Chr7g0241301
rosa_laevigata RLG00000001186 RLG00000001187 RLG00000001212 RLG00000001226 RLG00000001233 RLG00000001590 RLG00000003242 RLG00000003243 RLG00000003249 RLG00000008688 RLG00000008690
rosa_multiflora Rmu_co7966574.1_g000001 Rmu_co8120830.1_g000001 Rmu_co8142226.1_g000001 Rmu_co8293083.1_g000001 Rmu_co8351227.1_g000001 Rmu_co8380159.1_g000001 Rmu_co8456505.1_g000001 Rmu_sc0000676.1_g000025 Rmu_sc0000706.1_g000018 Rmu_sc0000706.1_g000040 Rmu_sc0000740.1_g000029 Rmu_sc0000740.1_g000032 Rmu_sc0000740.1_g000037 Rmu_sc0000740.1_g000040 Rmu_sc0000762.1_g000003 Rmu_sc0000762.1_g000019 Rmu_sc0000762.1_g000034 Rmu_sc0000762.1_g000069 Rmu_sc0000892.1_g000001 Rmu_sc0000945.1_g000040 Rmu_sc0001913.1_g000037 Rmu_sc0001913.1_g000041 Rmu_sc0001913.1_g000049 Rmu_sc0002775.1_g000017 Rmu_sc0002775.1_g000018 Rmu_sc0002775.1_g000020 Rmu_sc0002775.1_g000021 Rmu_sc0002775.1_g000022 Rmu_sc0002775.1_g000025 Rmu_sc0002775.1_g000026 Rmu_sc0002775.1_g000028 Rmu_sc0002775.1_g000030 Rmu_sc0004858.1_g000002 Rmu_sc0004864.1_g000022 Rmu_sc0005038.1_g000023 Rmu_sc0005840.1_g000014 Rmu_sc0006724.1_g000003 Rmu_sc0006824.1_g000005 Rmu_sc0007869.1_g000003 Rmu_sc0007869.1_g000005 Rmu_sc0007869.1_g000008 Rmu_sc0008646.1_g000007 Rmu_sc0009637.1_g000009 Rmu_sc0010500.1_g000002 Rmu_sc0010500.1_g000012 Rmu_sc0012403.1_g000025 Rmu_sc0013768.1_g000025 Rmu_sc0015625.1_g000001 Rmu_sc0019715.1_g000001 Rmu_sc0021133.1_g000002 Rmu_sc0021904.1_g000001 Rmu_sc0021904.1_g000003 Rmu_sc0021904.1_g000004 Rmu_sc0022054.1_g000001 Rmu_sc0026025.1_g000001 Rmu_sc0036828.1_g000002 Rmu_sc0041808.1_g000001 Rmu_ssc0000012.1_g000001
rosa_roxburghii Rroxscaffold_3G00226370 Rroxscaffold_3G00226860 Rroxscaffold_3G00226900 Rroxscaffold_3G00226930 Rroxscaffold_3G00226940 Rroxscaffold_3G00226970 Rroxscaffold_3G00226980 Rroxscaffold_3G00231530 Rroxscaffold_3G00237090 Rroxscaffold_3G00250760 Rroxscaffold_3G00250800
rosa_rugosa Rorug04G0082300 Rorug07G0101800 Rorug07G0102000 Rorug07G0102100 Rorug07G0221200 Rorug07G0245700 Rorug07G0245700 Rorug07G0245700 Rorug07G0263900 Rorug07G0264000 Rorug07G0264000 Rorug07G0264500 Rorug07G0282900 Rorug07G0282900 Rorug07G0283100 Rorug07G0283200.1 Rorug07G0283300 Rorug07G0283900 Rorug07G0284000 Rorug07G0284100 Rorug07G0284300 Rorug07G0284400 Rorug07G0284500 Rorug07G0285000 Rorug07G0285200 Rorug07G0285300 Rorug07G0285500 Rorug07G0286800 Rorug07G0286900 Rorug07G0287100 Rorug07G0287200
rosa_samantha Rh7AG236100 Rh7AG236200 Rh7AG236300 Rh7AG440000 Rh7AG440100 Rh7BG411900 Rh7BG412000 Rh7BG412600 Rh7CG251300 Rh7CG417700 Rh7CG460100 Rh7DG242100 Rh7DG242200 Rh7DG242300 Rh7DG429300
rosa_wichuraiana Rw0G005070 Rw0G010780 Rw0G012530 Rw7G020110 Rw7G020160 Rw7G020170 Rw7G033210 Rw7G033230 Rw7G033250 Rw7G034540 Rw7G035150 Rw7G036190 Rw7G036210 Rw7G036260 Rw7G036270 Rw7G036290 Rw7G036300 Rw7G036310 Rw7G036350 Rw7G036360 Rw7G036440 Rw7G036510 Rw7G036530 Rw7G036570 Rw7G036590 Rw7G036730

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 753, 1481
AccB7I CCANNNNNTGG 1 cut(s) 2646
AccBSI CCGCTC 1 cut(s) 2862
AccI GTMKAC 1 cut(s) 951
AccII CGCG 4 cut(s) 537, 642, 2266, 2997
AciI CCGC 9 cut(s) 78, 119, 1082, 1316, 2264, 2721, 2860, 3066, 3108
AclI AACGTT 1 cut(s) 798
AclWI GGATC 5 cut(s) 230, 341, 685, 1337, 2329
AcoI YGGCCR 4 cut(s) 505, 612, 1530, 2876
AcsI RAATTY 7 cut(s) 496, 702, 759, 1289, 2377, 2497, 2504
AcuI CTGAAG 3 cut(s) 58, 1133, 1656
AfaI GTAC 6 cut(s) 930, 1708, 1902, 2229, 2262, 2947
AfiI CCNNNNNNNGG 8 cut(s) 118, 1157, 1301, 1600, 2060, 2646, 2819, 3104
AflII CTTAAG 1 cut(s) 1085
AflIII ACRYGT 5 cut(s) 60, 319, 355, 1497, 3280
AhlI ACTAGT 1 cut(s) 1867
AjiI CACGTC 2 cut(s) 61, 320
AjnI CCWGG 6 cut(s) 141, 1003, 1567, 1696, 1896, 2122
AjuI GAANNNNNNNTTGG 4 cut(s) 976, 1008, 1256, 1288
Alw21I GWGCWC 1 cut(s) 2669
Alw26I GTCTC 2 cut(s) 1732, 3238
Alw44I GTGCAC 1 cut(s) 2665
AlwI GGATC 5 cut(s) 230, 341, 685, 1337, 2329
AlwNI CAGNNNCTG 1 cut(s) 491
Ama87I CYCGRG 3 cut(s) 259, 2195, 2812
AoxI GGCC 7 cut(s) 106, 505, 612, 1148, 1530, 1676, 2876
ApaLI GTGCAC 1 cut(s) 2665
ApeKI GCWGC 4 cut(s) 491, 737, 1762, 2100
ApoI RAATTY 7 cut(s) 496, 702, 759, 1289, 2377, 2497, 2504
AseI ATTAAT 1 cut(s) 1538
Asp700I GAANNNNTTC 2 cut(s) 585, 634
AspLEI GCGC 2 cut(s) 537, 2997
AspS9I GGNCC 4 cut(s) 106, 1001, 1942, 2139
AsuC2I CCSGG 5 cut(s) 203, 591, 623, 2813, 2814
AsuHPI GGTGA 6 cut(s) 295, 1971, 2042, 2098, 2828, 3272
AsuII TTCGAA 1 cut(s) 2555
AvaI CYCGRG 3 cut(s) 259, 2195, 2812
AvaII GGWCC 3 cut(s) 1001, 1942, 2139
BaeGI GKGCMC 1 cut(s) 2669
BalI TGGCCA 3 cut(s) 507, 614, 1532
BanII GRGCYC 1 cut(s) 1919
BarI GAAGNNNNNNTAC 2 cut(s) 287, 319
BauI CACGAG 2 cut(s) 1029, 2913
BbsI GAAGAC 2 cut(s) 229, 1497
Bbv12I GWGCWC 1 cut(s) 2669
BbvI GCAGC 4 cut(s) 478, 724, 1774, 2087
BccI CCATC 9 cut(s) 706, 878, 1386, 1418, 2703, 2777, 2813, 2897, 3218
BceAI ACGGC 2 cut(s) 2374, 2694
BciT130I CCWGG 6 cut(s) 143, 1005, 1569, 1698, 1898, 2124
BciVI GTATCC 2 cut(s) 131, 1673
BclI TGATCA 1 cut(s) 1855
BcnI CCSGG 5 cut(s) 203, 591, 623, 2813, 2814
BcoDI GTCTC 2 cut(s) 1732, 3238
BcuI ACTAGT 1 cut(s) 1867
BfaI CTAG 8 cut(s) 167, 1340, 1658, 1868, 1890, 2520, 2919, 3176
BfmI CTRYAG 2 cut(s) 2134, 2244
BfrI CTTAAG 1 cut(s) 1085
BfuI GTATCC 2 cut(s) 131, 1673
BglII AGATCT 1 cut(s) 2383
BisI GCNGC 5 cut(s) 120, 492, 738, 1763, 2101
BlsI GCNGC 5 cut(s) 121, 493, 739, 1764, 2102
BmcAI AGTACT 1 cut(s) 2229
Bme18I GGWCC 3 cut(s) 1001, 1942, 2139
BmeT110I CYCGRG 3 cut(s) 259, 2195, 2812
BmgBI CACGTC 2 cut(s) 61, 320
BmgT120I GGNCC 4 cut(s) 106, 1001, 1942, 2139
BmiI GGNNCC 3 cut(s) 1592, 1918, 2613
BmrI ACTGGG 1 cut(s) 320
BmsI GCATC 7 cut(s) 1463, 1811, 2246, 2503, 2525, 3253, 3275
BmuI ACTGGG 1 cut(s) 320
BpiI GAAGAC 2 cut(s) 229, 1497
BplI GAGNNNNNCTC 2 cut(s) 463, 495
BpmI CTGGAG 1 cut(s) 1590
Bpu10I CCTNAGC 1 cut(s) 3156
Bpu14I TTCGAA 1 cut(s) 2555
BpuEI CTTGAG 3 cut(s) 88, 369, 1730
BpuMI CCSGG 5 cut(s) 203, 591, 623, 2813, 2814
Bsa29I ATCGAT 1 cut(s) 153
BsaBI GATNNNNATC 3 cut(s) 227, 2907, 3216
BsaJI CCNNGG 8 cut(s) 1673, 1682, 1697, 1791, 2123, 2196, 2811, 2812
BsaXI ACNNNNNCTCC 1 cut(s) 32
Bsc4I CCNNNNNNNGG 8 cut(s) 118, 1157, 1301, 1600, 2060, 2646, 2819, 3104
Bse118I RCCGGY 1 cut(s) 1593
Bse1I ACTGG 2 cut(s) 315, 876
Bse3DI GCAATG 2 cut(s) 100, 473
Bse8I GATNNNNATC 3 cut(s) 227, 2907, 3216
BseBI CCWGG 6 cut(s) 143, 1005, 1569, 1698, 1898, 2124
BseCI ATCGAT 1 cut(s) 153
BseDI CCNNGG 8 cut(s) 1673, 1682, 1697, 1791, 2123, 2196, 2811, 2812
BseGI GGATG 8 cut(s) 26, 717, 1397, 1433, 2518, 2714, 2908, 3237
BseJI GATNNNNATC 3 cut(s) 227, 2907, 3216
BseLI CCNNNNNNNGG 8 cut(s) 118, 1157, 1301, 1600, 2060, 2646, 2819, 3104
BseMI GCAATG 2 cut(s) 100, 473
BseMII CTCAG 6 cut(s) 852, 1727, 2403, 2434, 2598, 2940
BseNI ACTGG 2 cut(s) 315, 876
BseRI GAGGAG 3 cut(s) 10, 22, 1659
BseSI GKGCMC 1 cut(s) 2669
BseXI GCAGC 4 cut(s) 478, 724, 1774, 2087
BseYI CCCAGC 2 cut(s) 2414, 2466
BsgI GTGCAG 2 cut(s) 319, 1781
Bsh1236I CGCG 4 cut(s) 537, 642, 2266, 2997
BshFI GGCC 7 cut(s) 108, 507, 614, 1150, 1532, 1678, 2878
BshVI ATCGAT 1 cut(s) 153
BsiHKAI GWGCWC 1 cut(s) 2669
BsiHKCI CYCGRG 3 cut(s) 259, 2195, 2812
BsiSI CCGG 5 cut(s) 202, 590, 623, 1594, 2813
BslFI GGGAC 1 cut(s) 3197
BslI CCNNNNNNNGG 8 cut(s) 118, 1157, 1301, 1600, 2060, 2646, 2819, 3104
BsmAI GTCTC 2 cut(s) 1732, 3238
BsmFI GGGAC 1 cut(s) 3197
BsmI GAATGC 1 cut(s) 411
BsnI GGCC 7 cut(s) 108, 507, 614, 1150, 1532, 1678, 2878
BsoBI CYCGRG 3 cut(s) 259, 2195, 2812
Bsp119I TTCGAA 1 cut(s) 2555
Bsp1286I GDGCHC 2 cut(s) 1919, 2669
BspACI CCGC 9 cut(s) 78, 119, 1082, 1316, 2264, 2721, 2860, 3066, 3108
BspANI GGCC 7 cut(s) 108, 507, 614, 1150, 1532, 1678, 2878
BspCNI CTCAG 6 cut(s) 853, 1728, 2402, 2433, 2599, 2941
BspDI ATCGAT 1 cut(s) 153
BspFNI CGCG 4 cut(s) 537, 642, 2266, 2997
BspHI TCATGA 1 cut(s) 291
BspLI GGNNCC 3 cut(s) 1592, 1918, 2613
BspPI GGATC 5 cut(s) 230, 341, 685, 1337, 2329
BspT104I TTCGAA 1 cut(s) 2555
BspTI CTTAAG 1 cut(s) 1085
BsrBI CCGCTC 1 cut(s) 2862
BsrDI GCAATG 2 cut(s) 100, 473
BsrFI RCCGGY 1 cut(s) 1593
BsrI ACTGG 2 cut(s) 315, 876
BssAI RCCGGY 1 cut(s) 1593
BssECI CCNNGG 8 cut(s) 1673, 1682, 1697, 1791, 2123, 2196, 2811, 2812
BssSI CACGAG 2 cut(s) 1029, 2913
BssT1I CCWWGG 3 cut(s) 1673, 1682, 1791
Bst2BI CACGAG 2 cut(s) 1029, 2913
Bst2UI CCWGG 6 cut(s) 143, 1005, 1569, 1698, 1898, 2124
Bst4CI ACNGT 4 cut(s) 198, 1196, 1486, 2275
Bst6I CTCTTC 4 cut(s) 207, 399, 2004, 2124
BstAFI CTTAAG 1 cut(s) 1085
BstAPI GCANNNNNTGC 1 cut(s) 1516
BstBI TTCGAA 1 cut(s) 2555
BstC8I GCNNGC 1 cut(s) 1131
BstENI CCTNNNNNAGG 2 cut(s) 1155, 1299
BstF5I GGATG 8 cut(s) 26, 717, 1397, 1433, 2518, 2714, 2908, 3237
BstFNI CGCG 4 cut(s) 537, 642, 2266, 2997
BstHHI GCGC 2 cut(s) 537, 2997
BstMAI GTCTC 2 cut(s) 1732, 3238
BstMWI GCNNNNNNNGC 6 cut(s) 105, 743, 832, 1516, 2859, 3058
BstNI CCWGG 6 cut(s) 143, 1005, 1569, 1698, 1898, 2124
BstNSI RCATGY 4 cut(s) 359, 1133, 1696, 3284
BstSFI CTRYAG 2 cut(s) 2134, 2244
BstSLI GKGCMC 1 cut(s) 2669
BstUI CGCG 4 cut(s) 537, 642, 2266, 2997
BstV1I GCAGC 4 cut(s) 478, 724, 1774, 2087
BstV2I GAAGAC 2 cut(s) 229, 1497
BstX2I RGATCY 3 cut(s) 222, 1342, 2383
BstXI CCANNNNNNTGG 1 cut(s) 1260
BstYI RGATCY 3 cut(s) 222, 1342, 2383
Bsu15I ATCGAT 1 cut(s) 153
BsuI GTATCC 2 cut(s) 131, 1673
BsuRI GGCC 7 cut(s) 108, 507, 614, 1150, 1532, 1678, 2878
BsuTUI ATCGAT 1 cut(s) 153
BtrI CACGTC 2 cut(s) 61, 320
BtsCI GGATG 8 cut(s) 26, 717, 1397, 1433, 2518, 2714, 2908, 3237
BtsIMutI CAGTG 5 cut(s) 883, 1462, 2191, 2428, 2604
Cac8I GCNNGC 1 cut(s) 1131
CaiI CAGNNNCTG 1 cut(s) 491
CciI TCATGA 1 cut(s) 291
CfoI GCGC 2 cut(s) 537, 2997
Cfr10I RCCGGY 1 cut(s) 1593
Cfr13I GGNCC 4 cut(s) 106, 1001, 1942, 2139
Cfr9I CCCGGG 1 cut(s) 2812
ClaI ATCGAT 1 cut(s) 153
CsiI ACCWGGT 1 cut(s) 1896
Csp6I GTAC 6 cut(s) 929, 1707, 1901, 2228, 2261, 2946
CspCI CAANNNNNGTGG 2 cut(s) 3238, 3273
CviQI GTAC 6 cut(s) 929, 1707, 1901, 2228, 2261, 2946
DraI TTTAAA 1 cut(s) 1830
EaeI YGGCCR 4 cut(s) 505, 612, 1530, 2876
Eam1104I CTCTTC 4 cut(s) 207, 399, 2004, 2124
EarI CTCTTC 4 cut(s) 207, 399, 2004, 2124
Eco130I CCWWGG 3 cut(s) 1673, 1682, 1791
Eco24I GRGCYC 1 cut(s) 1919
Eco32I GATATC 2 cut(s) 1163, 2217
Eco47I GGWCC 3 cut(s) 1001, 1942, 2139
Eco57I CTGAAG 3 cut(s) 58, 1133, 1656
Eco88I CYCGRG 3 cut(s) 259, 2195, 2812
EcoNI CCTNNNNNAGG 2 cut(s) 1155, 1299
EcoO109I RGGNCCY 1 cut(s) 1942
EcoRII CCWGG 6 cut(s) 141, 1003, 1567, 1696, 1896, 2122
EcoRV GATATC 2 cut(s) 1163, 2217
EcoT14I CCWWGG 3 cut(s) 1673, 1682, 1791
EcoT22I ATGCAT 4 cut(s) 1512, 2518, 3139, 3268
EcoT38I GRGCYC 1 cut(s) 1919
ErhI CCWWGG 3 cut(s) 1673, 1682, 1791
FalI AAGNNNNNCTT 8 cut(s) 531, 563, 819, 851, 1149, 1181, 1810, 1842
FaqI GGGAC 1 cut(s) 3197
FauI CCCGC 1 cut(s) 3059
FbaI TGATCA 1 cut(s) 1855
FblI GTMKAC 1 cut(s) 951
Fnu4HI GCNGC 5 cut(s) 120, 492, 738, 1763, 2101
FokI GGATG 8 cut(s) 13, 724, 1404, 1440, 2525, 2721, 2895, 3224
FriOI GRGCYC 1 cut(s) 1919
Fsp4HI GCNGC 5 cut(s) 120, 492, 738, 1763, 2101
FspBI CTAG 8 cut(s) 167, 1340, 1658, 1868, 1890, 2520, 2919, 3176
GlaI GCGC 2 cut(s) 536, 2996
GluI GCNGC 5 cut(s) 120, 492, 738, 1763, 2101
GsaI CCCAGC 2 cut(s) 2418, 2470
GsuI CTGGAG 1 cut(s) 1590
HaeIII GGCC 7 cut(s) 108, 507, 614, 1150, 1532, 1678, 2878
HapII CCGG 5 cut(s) 202, 590, 623, 1594, 2813
HhaI GCGC 2 cut(s) 537, 2997
Hin6I GCGC 2 cut(s) 535, 2995
HinP1I GCGC 2 cut(s) 535, 2995
HindIII AAGCTT 3 cut(s) 545, 833, 1327
HinfI GANTC 9 cut(s) 630, 863, 1452, 2107, 2155, 2287, 2348, 2366, 2591
HpaII CCGG 5 cut(s) 202, 590, 623, 1594, 2813
HphI GGTGA 6 cut(s) 295, 1971, 2042, 2098, 2828, 3272
Hpy166II GTNNAC 8 cut(s) 85, 952, 1043, 1057, 1069, 1469, 2089, 2667
Hpy188I TCNGA 7 cut(s) 396, 844, 1113, 1549, 1966, 2044, 2154
Hpy8I GTNNAC 8 cut(s) 85, 952, 1043, 1057, 1069, 1469, 2089, 2667
Hpy99I CGWCG 2 cut(s) 62, 321
HpyAV CCTTC 6 cut(s) 360, 1151, 1640, 1933, 2828, 3190
HpyCH4III ACNGT 4 cut(s) 198, 1196, 1486, 2275
HpyCH4IV ACGT 9 cut(s) 60, 319, 439, 687, 798, 919, 1497, 1758, 2697
HpyF10VI GCNNNNNNNGC 6 cut(s) 105, 743, 832, 1516, 2859, 3058
HpySE526I ACGT 9 cut(s) 60, 319, 439, 687, 798, 919, 1497, 1758, 2697
HspAI GCGC 2 cut(s) 535, 2995
Ksp22I TGATCA 1 cut(s) 1855
LmnI GCTCC 2 cut(s) 1922, 2468
Lsp1109I GCAGC 4 cut(s) 478, 724, 1774, 2087
LweI GCATC 7 cut(s) 1463, 1811, 2246, 2503, 2525, 3253, 3275
MabI ACCWGGT 1 cut(s) 1896
MaeI CTAG 8 cut(s) 167, 1340, 1658, 1868, 1890, 2520, 2919, 3176
MaeII ACGT 9 cut(s) 60, 319, 439, 687, 798, 919, 1497, 1758, 2697
MaeIII GTNAC 7 cut(s) 1430, 1457, 1923, 2352, 2536, 2952, 2983
MbiI CCGCTC 1 cut(s) 2862
MfeI CAATTG 1 cut(s) 207
MflI RGATCY 3 cut(s) 222, 1342, 2383
MhlI GDGCHC 2 cut(s) 1919, 2669
MlsI TGGCCA 3 cut(s) 507, 614, 1532
MluNI TGGCCA 3 cut(s) 507, 614, 1532
MlyI GAGTC 3 cut(s) 872, 2149, 2360
MmeI TCCRAC 4 cut(s) 607, 679, 940, 1254
Mox20I TGGCCA 3 cut(s) 507, 614, 1532
Mph1103I ATGCAT 4 cut(s) 1512, 2518, 3139, 3268
MroXI GAANNNNTTC 2 cut(s) 585, 634
MscI TGGCCA 3 cut(s) 507, 614, 1532
MslI CAYNNNNRTG 3 cut(s) 114, 1064, 2913
Msp20I TGGCCA 3 cut(s) 507, 614, 1532
MspA1I CMGCKG 2 cut(s) 78, 737
MspCI CTTAAG 1 cut(s) 1085
MspI CCGG 5 cut(s) 202, 590, 623, 1594, 2813
MunI CAATTG 1 cut(s) 207
Mva1269I GAATGC 1 cut(s) 411
MvaI CCWGG 6 cut(s) 143, 1005, 1569, 1698, 1898, 2124
MvnI CGCG 4 cut(s) 537, 642, 2266, 2997
MwoI GCNNNNNNNGC 6 cut(s) 105, 743, 832, 1516, 2859, 3058
NciI CCSGG 5 cut(s) 203, 591, 623, 2813, 2814
NlaIV GGNNCC 3 cut(s) 1592, 1918, 2613
NmuCI GTSAC 2 cut(s) 1457, 1923
NsiI ATGCAT 4 cut(s) 1512, 2518, 3139, 3268
NspI RCATGY 4 cut(s) 359, 1133, 1696, 3284
NspV TTCGAA 1 cut(s) 2555
PaeI GCATGC 1 cut(s) 1133
PaeR7I CTCGAG 1 cut(s) 259
PagI TCATGA 1 cut(s) 291
PciI ACATGT 2 cut(s) 355, 3280
PcsI WCGNNNNNNNCGW 1 cut(s) 159
PctI GAATGC 1 cut(s) 411
PdmI GAANNNNTTC 2 cut(s) 585, 634
PfeI GAWTC 6 cut(s) 630, 1452, 2107, 2287, 2348, 2591
PflFI GACNNNGTC 3 cut(s) 689, 862, 1406
PflMI CCANNNNNTGG 1 cut(s) 2646
PfoI TCCNGGA 1 cut(s) 1003
PkrI GCNGC 5 cut(s) 121, 493, 739, 1764, 2102
PleI GAGTC 3 cut(s) 871, 2149, 2360
PpsI GAGTC 3 cut(s) 871, 2149, 2360
PpuMI RGGWCCY 1 cut(s) 1942
PscI ACATGT 2 cut(s) 355, 3280
PshBI ATTAAT 1 cut(s) 1538
PsiI TTATAA 2 cut(s) 753, 1481
Psp1406I AACGTT 1 cut(s) 798
Psp5II RGGWCCY 1 cut(s) 1942
Psp6I CCWGG 6 cut(s) 141, 1003, 1567, 1696, 1896, 2122
PspFI CCCAGC 2 cut(s) 2414, 2466
PspGI CCWGG 6 cut(s) 141, 1003, 1567, 1696, 1896, 2122
PspN4I GGNNCC 3 cut(s) 1592, 1918, 2613
PspPI GGNCC 4 cut(s) 106, 1001, 1942, 2139
PspPPI RGGWCCY 1 cut(s) 1942
PspXI VCTCGAGB 1 cut(s) 259
PstNI CAGNNNCTG 1 cut(s) 491
PsuI RGATCY 3 cut(s) 222, 1342, 2383
PsyI GACNNNGTC 3 cut(s) 689, 862, 1406
PvuII CAGCTG 1 cut(s) 737
RsaI GTAC 6 cut(s) 930, 1708, 1902, 2229, 2262, 2947
RsaNI GTAC 6 cut(s) 929, 1707, 1901, 2228, 2261, 2946
RseI CAYNNNNRTG 3 cut(s) 114, 1064, 2913
SatI GCNGC 5 cut(s) 120, 492, 738, 1763, 2101
Sau96I GGNCC 4 cut(s) 106, 1001, 1942, 2139
ScaI AGTACT 1 cut(s) 2229
SchI GAGTC 3 cut(s) 872, 2149, 2360
SduI GDGCHC 2 cut(s) 1919, 2669
SexAI ACCWGGT 1 cut(s) 1896
SfaNI GCATC 7 cut(s) 1463, 1811, 2246, 2503, 2525, 3253, 3275
SfcI CTRYAG 2 cut(s) 2134, 2244
Sfr274I CTCGAG 1 cut(s) 259
SfuI TTCGAA 1 cut(s) 2555
SinI GGWCC 3 cut(s) 1001, 1942, 2139
SlaI CTCGAG 1 cut(s) 259
SmaI CCCGGG 1 cut(s) 2814
SmiMI CAYNNNNRTG 3 cut(s) 114, 1064, 2913
SmlI CTYRAG 5 cut(s) 67, 259, 384, 1085, 1745
SmoI CTYRAG 5 cut(s) 67, 259, 384, 1085, 1745
SpeI ACTAGT 1 cut(s) 1867
SphI GCATGC 1 cut(s) 1133
SsiI CCGC 9 cut(s) 78, 119, 1082, 1316, 2264, 2721, 2860, 3066, 3108
SspI AATATT 2 cut(s) 333, 1419
SspMI CTAG 8 cut(s) 167, 1340, 1658, 1868, 1890, 2520, 2919, 3176
StyI CCWWGG 3 cut(s) 1673, 1682, 1791
TaaI ACNGT 4 cut(s) 198, 1196, 1486, 2275
TaiI ACGT 9 cut(s) 63, 322, 442, 690, 801, 922, 1500, 1761, 2700
TaqI TCGA 6 cut(s) 153, 260, 1522, 2324, 2555, 2769
TatI WGTACW 2 cut(s) 1706, 2227
TauI GCSGC 1 cut(s) 122
TfiI GAWTC 6 cut(s) 630, 1452, 2107, 2287, 2348, 2591
TscAI CASTG 5 cut(s) 883, 1462, 2191, 2428, 2611
TseFI GTSAC 2 cut(s) 1457, 1923
TseI GCWGC 4 cut(s) 491, 737, 1762, 2100
Tsp45I GTSAC 2 cut(s) 1457, 1923
TspGWI ACGGA 4 cut(s) 387, 593, 1454, 2137
TspMI CCCGGG 1 cut(s) 2812
TspRI CASTG 5 cut(s) 883, 1462, 2191, 2428, 2611
Tth111I GACNNNGTC 3 cut(s) 689, 862, 1406
Van91I CCANNNNNTGG 1 cut(s) 2646
Vha464I CTTAAG 1 cut(s) 1085
VneI GTGCAC 1 cut(s) 2665
VpaK11BI GGWCC 3 cut(s) 1001, 1942, 2139
VspI ATTAAT 1 cut(s) 1538
XagI CCTNNNNNAGG 2 cut(s) 1155, 1299
XapI RAATTY 7 cut(s) 496, 702, 759, 1289, 2377, 2497, 2504
XbaI TCTAGA 1 cut(s) 166
XceI RCATGY 4 cut(s) 359, 1133, 1696, 3284
XhoI CTCGAG 1 cut(s) 259
XmaI CCCGGG 1 cut(s) 2812
XmiI GTMKAC 1 cut(s) 951
XmnI GAANNNNTTC 2 cut(s) 585, 634
XspI CTAG 8 cut(s) 167, 1340, 1658, 1868, 1890, 2520, 2919, 3176
ZrmI AGTACT 1 cut(s) 2229
Zsp2I ATGCAT 4 cut(s) 1512, 2518, 3139, 3268
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.