MD02G1052800.v1.1

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0000055
Physical Location & Seq
Reverse (-)
4323093 .. 4333203
10111 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1052800.v1.1.491

Sequence Viewer

Length: 3771 bp
ATGACAACCCACGAAGCCTCCTCTTCATCCTCCTCCAAGTCAAAACTTTGGAATTACGACGTGTTCTTGAGCTTTAGCGGTGTAGACACACGCAATGGCTTCACGGGCCACCTCCACGCGGCATTAACAGACAGGGGATACCAGGCTTATATTGATGAGGACGATCTCAAAAGAGGGGAAGAAATACCAAAAGAACTGCTCCAGGCAATCGAAGAATCGAAGACCTCTATCATTGTCTTCTCAGAGAGGTATGCGGATTCGAGTTGGTGTCTTAACGAGCTGGTGAAGATCATGGAGTGCAGAGACAAACTGGGGCGACATGTTTTGCCAATATTCTATCACATTGATCCTTCACATGTCAGGAAGCAGAACGGAGTTTTAGCCCAAGCATTTCAGAAGCACAAAAATGACATCAGTGAAGAAAAAGATGACAAGAAACGTGAAGAAAAACGAGAAAGGGTAGAGCAGTGGATAAAGGCTCTTACAGAAGCTGCAAAGTCAGCCGAAGAATTTCAAAAGCACGAAGAAGGCGTCGGTGAAGAAAAGGATGACAAGAAACGTGAAGCTAAACAAGAAGAGGTAAAGCAGTGGGGAGAGGTTCTTACAAAAGCTGCAAATTTGTCTGGCTACCATCTTCAAATCACTGATAATAGGCGCGAAGCAAAGCTTATTAGAGAAATTGTTGACAAGATTATCCCAAAATGGCTTCCGAGCGCAGAAAATTTACATTTGGCCAAGCACCCAGTTGGAATATATACTCGCATTCAAGATATCATCACTTATCTTTCAAATGGTGGATCATATGTTGTTCGCATGGTTGGAATTTGGGGGATTGGTGGATTGGGTAAAACAACAGCTGCCAAAGCCATTTATAACCAACTTCATCATAAGTTCCAATTCAAAAGTTTCCTTGCCAACGTTAGTGAATGTGATCTGGTTGATTTGCAAGGAAGACTTGTTTCTGACATCTTGAAACATACCGAGTCTAAGATAACCAGTGTTGATAGAGGTATCAGTCTGATAAAAAATCATCTCCAACGTAGAAGCGTGCTTGTCATTATTGACAATGTAGACAAAGTGGAACAACTAGATGCAATAGCTGGAAATCGTGATTGGTTTGGCCCAGGAAGTAGAATTATCATAACGACACGAGATGAACGTTTTCTAAAGCAAGTGAACATGAAAGTGGACAAGACATATTCACTTAAGGAAATGAATGAAGAAGAAGCTCTGAAGCTCTTTAGTTGGCATGCCTTTGGAAAGAGTTGGCCTAATGAAGGATATCTTGAACTCTCAAAAGAGAAAAGTCAGTTGGAGAAATTGAAAAAATTTCCTGATGAAGGAATAATGAAACCACTAAGAGTAAGCTTTGAAAGGCTAGATCCTACACAAAAGGATATATTCCTTGACATATCTTGTTTCTTTATTGGATGGGATAAGGACTATGTCGCAAAAATATTAGATGGATGTAAGTTTTTTGCAACAATAGGAATCAATGTCCTCTGTGAACGATGCCTTATCACTATTGAACACAAGAAGTTGAATATGCATGATTTGCTTCAAGAAATGGCCAAATTAATCATTTCTGAAAAATCCCCTCGTCACCCTGGAGAATGGAGTAGGTTGTGGAACCAGCAAGAGGTCATTGATGTATTGAGAGATAAATTTGGAACTGAAGAAGTTAAAGGACTTGCTCTTAATTTTTCTGGAGATCATTGGCTGCAAAGGCTTCGAAGCTTCAGTACAGAAGCATTTGCCAATATGAAGAAACTGAGACTGCTGCAGCTCTACAACGTGCAGCTCAATGGAGAATACAAACATCTTTCCAAAGAGTTAATATGGTTGCGTTGGTTTGGATGCCCTTTAAAGTCCATACCAGATCACTTCTTTGATCAACCAAGAGCACTGGTTATTTTAGAGATAGCGTATAGCAGACTTGTACAAGTTTGGGAGGGCTCCAAGTCGCTTGGAAACTTGAAAATCCTTAATCTCAGTTATTGCCTTGACTTAAAAAAATCACCAGACTTTTCAAATCTCCTAAATCTTGAAGAGTTGATATTGGAATGGTGTAACAGTTTGTCCGAGATTCACCCCTCCATTTGTCATCTTAAAAAACTTTCTTTGGTGAACCTCACAGGCTGCCAGAATCTTATTTCTCTTCCTGGGGATTTCTACAAGTCCAAATCCGTTCAGACTCTTGTTCTTAATGGATGTCAGCAATTCAGTGAACTGCCCGAGGATTTAGGGAAGATGACATCATTGAGAGTACTTATAGCATCTTCCACAGCCATAAGACAAGTACCCTGTTCCATAGTAAGATTGAAGAATCTCACTCATTTATCTCTAGCGTCTGTGGAATCGAAGTTTACTTTGCAGTTTCCCAATTCGTTAGACGGAAATTTAGATCTCTCAAAACATTATTTTCATACCCTACCCAGCCTATGTGGTCTTTCAAAACTTGAAAATTTGCAGTTAAGTAACTGCTGTAACCTTCATACCCTACCCGGCCTGAGTGGTCTTTCACAACTTAAAACTCTGGAGTTAAATCACTGCAGTAACTTATACACCTTACCCAGCCTCAATGGTCTTTCAAATCTTAAAACTCTGGGGTTAAGTCATTGCAGTAACTTACACACCCTACCCAGCCTCAATGGTCTTTCAAAGCTTAAAACTCTGGGGTTAAGTCGCTGCAGTAACTTACACTCCCTACCCAGCCTCAATGGTCTTTCAAAGCTTAAAACTCTGGAGTTAAGTATGGAGTTAAGTCACTGCAGTAACTTACACACCCTACCCAGCCTCAATGGTCTTTCAAATCTTAAAACTCTGGGGTTAAGTCAATGCAGTAACTTACACACCCTACCCAGCCTTAATTGTCTTTCAAATCTTAAAACTCTATGGTTAAGTCACTGCAGTAACTTACACACCCTACCTAGCCTCAATGGTCTTTCAAATCTTAAAACCCTGGAGTTAAGTCACTGCAGTAACTTACACATCCTACCCAGCCTCAATGGTCTTTCAAATCTTAAAACTCTAGGGTTAAGTCACTGCAGTAACTTACACATCCTACCCAGCCTCAATGATCTTTCAAATCTTAAAACTCTGTGGTTAAGTCACTGCAGTAACTTACACACCCTACCCAGCCTCAATGGTCTTTCAAATCTTAAAACTATGTGGTTAAATCACTGTGGTAACTTACACACCCTTCCCTGCCTCAATGGTCTTTCAAATCTTGAAACTCTGGAATTAAGTCACAGCAGTAACTTACACACCCTACCCAGCCTCAATGGTCTTTCAAATCTTAAAACTCTGGAGTTAAGTCACTGCAGTATCTTACACACCCTACCCAGCCTCAATGGTCTTTCAAAGCTTGAAGGTATGTGGTTAAGTTATTGTTCCCAGCTTCATACAATCTATGATTTACCAACAAATTTGAAATTTCTGGATGCTTATGGTTGCTGTTGTTTGGTAAAAATGCCCAATTTTTCGAAAATGTCAAATATGAGAGAACTGAATGTAACTGGTTCACACGCACTCACAGAGATTCCGGACTTGGATAAGTCATTAAACTCCATGAAATGGATTGTAATGCAAAAGTGCACCAATCTCACAGCTGATTTTAGGAGGAAGGTCCTAAAGGGATGGACTTCTTACGGATATGCTGGCATTTTTCTCGATGCGAATTATGTTCCTGATTGGTTCGAGTTTGTCAACGATGGTGATAAAGTCAGTTTTGATACCCCCCAAGTGATGGTCGTAATTTTGAAGGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

1257

Amino Acids

142.03

Weight (kDa)

8.3

Isoelectric Point (pI)

34.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 18 - 173 2.1e-44 TIR domain
TIR_2 PF13676 21 - 117 2.8e-16 TIR domain
NB-ARC PF00931 255 - 421 7.4e-17 NB-ARC domain
WHD_ROQ1 PF23282 464 - 532 3.5e-14 Disease resistance protein Roq1-like, winged-helix domain
LRR_14 PF23598 973 - 1170 3.3e-06 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000055)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G36930 AT5G36930 AT5G36930 AT5G36930
fragaria_vesca FvH4_3g45361 FvH4_3g45380 FvH4_5g21061 FvH4_5g21062 FvH4_5g21063 FvH4_5g32050 FvH4_5g32970 FvH4_5g32990 FvH4_5g34190 FvH4_5g34210 FvH4_5g34210 FvH4_5g34210 FvH4_5g34210 FvH4_5g34210 FvH4_5g34210 FvH4_5g34211 FvH4_5g34240 FvH4_5g34250
malus_domestica MD00G1011700.v1.1 MD00G1026100.v1.1 MD00G1026500.v1.1 MD00G1027000.v1.1 MD00G1027100.v1.1 MD00G1027700.v1.1 MD00G1027800.v1.1 MD00G1028000.v1.1 MD00G1220000.v1.1 MD02G1024400.v1.1 MD02G1024800.v1.1 MD02G1026000.v1.1 MD02G1026100.v1.1 MD02G1031800.v1.1 MD02G1038500.v1.1 MD02G1038700.v1.1 MD02G1039200.v1.1 MD02G1040600.v1.1 MD02G1040900.v1.1 MD02G1041200.v1.1 MD02G1041300.v1.1 MD02G1041700.v1.1 MD02G1042000.v1.1 MD02G1043800.v1.1 MD02G1043900.v1.1 MD02G1044300.v1.1 MD02G1045200.v1.1 MD02G1045300.v1.1 MD02G1051200.v1.1 MD02G1052200.v1.1 MD02G1052800.v1.1 MD02G1053100.v1.1 MD02G1053600.v1.1 MD02G1055500.v1.1 MD02G1063300.v1.1 MD02G1063400.v1.1 MD02G1063800.v1.1 MD02G1306500.v1.1 MD02G1306600.v1.1 MD02G1306700.v1.1 MD02G1306900.v1.1 MD02G1307200.v1.1 MD04G1011700.v1.1 MD05G1315400.v1.1 MD05G1316000.v1.1 MD05G1316700.v1.1 MD05G1317000.v1.1 MD05G1317300.v1.1 MD05G1317600.v1.1 MD05G1317700.v1.1 MD07G1102800.v1.1 MD07G1238200.v1.1 MD08G1175900.v1.1 MD08G1203600.v1.1 MD09G1024200.v1.1 MD09G1039600.v1.1 MD09G1044800.v1.1 MD09G1045100.v1.1 MD10G1038700.v1.1 MD10G1039000.v1.1 MD10G1039200.v1.1 MD10G1040000.v1.1 MD10G1040200.v1.1 MD10G1040600.v1.1 MD10G1040800.v1.1 MD10G1297300.v1.1 MD12G1247500.v1.1 MD12G1247900.v1.1 MD12G1248300.v1.1 MD12G1248800.v1.1 MD15G1103700.v1.1 MD15G1103800.v1.1 MD15G1104000.v1.1 MD15G1179600.v1.1 MD15G1182500.v1.1 MD16G1068200.v1.1 MD16G1068300.v1.1 MD17G1024600.v1.1 MD17G1024700.v1.1 MD17G1025000.v1.1 MD17G1025900.v1.1
prunus_persica Prupe.1G539800_v2.0.a1 Prupe.1G550800_v2.0.a1 Prupe.1G550800_v2.0.a1 Prupe.1G550800_v2.0.a1 Prupe.1G550800_v2.0.a1 Prupe.1G550800_v2.0.a1 Prupe.1G550800_v2.0.a1 Prupe.1G557100_v2.0.a1 Prupe.1G557200_v2.0.a1 Prupe.1G557200_v2.0.a1 Prupe.1G557300_v2.0.a1
pyrus_communis pycom02g02080 pycom02g02090 pycom02g02110 pycom02g03220 pycom02g03260 pycom02g03370 pycom02g03390 pycom02g03410 pycom02g03420 pycom02g03450 pycom02g03500 pycom02g03730 pycom02g03770 pycom02g04400 pycom02g04410 pycom02g04430 pycom02g04450 pycom02g04460 pycom02g04490 pycom02g05200 pycom02g25710 pycom02g25750 pycom02g25790 pycom02g25810 pycom02g25830 pycom04g00920 pycom04g00930 pycom04g00940 pycom05g29300 pycom05g29310 pycom05g29380 pycom05g29410 pycom07g06310 pycom07g21420 pycom08g15070 pycom08g17540 pycom08g17560 pycom08g17570 pycom10g02670 pycom10g02680 pycom10g02700 pycom10g02770 pycom10g02790 pycom10g02800 pycom10g02810 pycom10g25020 pycom10g25030 pycom10g25050 pycom10g25080 pycom111g01990 pycom111g02000 pycom111g02030 pycom111g02040 pycom111g02050 pycom111g02060 pycom12g22710 pycom12g22720 pycom12g22730 pycom12g22740 pycom12g22750 pycom12g22770 pycom12g22780 pycom12g22790 pycom12g22800 pycom12g22810 pycom12g22830 pycom12g22840 pycom12g22860 pycom15g09440 pycom15g09480 pycom15g16120 pycom15g32380 pycom16g05970 pycom17g02100
rosa_chinensis RchiOBHm_Chr2g0144661 RchiOBHm_Chr4g0407491 RchiOBHm_Chr4g0407501 RchiOBHm_Chr4g0412381 RchiOBHm_Chr7g0208191 RchiOBHm_Chr7g0208201 RchiOBHm_Chr7g0208211 RchiOBHm_Chr7g0208221 RchiOBHm_Chr7g0228071 RchiOBHm_Chr7g0228121 RchiOBHm_Chr7g0230971 RchiOBHm_Chr7g0230981 RchiOBHm_Chr7g0233901 RchiOBHm_Chr7g0233951 RchiOBHm_Chr7g0233961 RchiOBHm_Chr7g0233991 RchiOBHm_Chr7g0234021 RchiOBHm_Chr7g0234151 RchiOBHm_Chr7g0234161 RchiOBHm_Chr7g0234241 RchiOBHm_Chr7g0234501 RchiOBHm_Chr7g0234641 RchiOBHm_Chr7g0234771 RchiOBHm_Chr7g0234781 RchiOBHm_Chr7g0234991 RchiOBHm_Chr7g0241291 RchiOBHm_Chr7g0241301
rosa_laevigata RLG00000001186 RLG00000001187 RLG00000001212 RLG00000001226 RLG00000001233 RLG00000001590 RLG00000003242 RLG00000003243 RLG00000003249 RLG00000008688 RLG00000008690
rosa_multiflora Rmu_co7966574.1_g000001 Rmu_co8120830.1_g000001 Rmu_co8142226.1_g000001 Rmu_co8293083.1_g000001 Rmu_co8351227.1_g000001 Rmu_co8380159.1_g000001 Rmu_co8456505.1_g000001 Rmu_sc0000676.1_g000025 Rmu_sc0000706.1_g000018 Rmu_sc0000706.1_g000040 Rmu_sc0000740.1_g000029 Rmu_sc0000740.1_g000032 Rmu_sc0000740.1_g000037 Rmu_sc0000740.1_g000040 Rmu_sc0000762.1_g000003 Rmu_sc0000762.1_g000019 Rmu_sc0000762.1_g000034 Rmu_sc0000762.1_g000069 Rmu_sc0000892.1_g000001 Rmu_sc0000945.1_g000040 Rmu_sc0001913.1_g000037 Rmu_sc0001913.1_g000041 Rmu_sc0001913.1_g000049 Rmu_sc0002775.1_g000017 Rmu_sc0002775.1_g000018 Rmu_sc0002775.1_g000020 Rmu_sc0002775.1_g000021 Rmu_sc0002775.1_g000022 Rmu_sc0002775.1_g000025 Rmu_sc0002775.1_g000026 Rmu_sc0002775.1_g000028 Rmu_sc0002775.1_g000030 Rmu_sc0004858.1_g000002 Rmu_sc0004864.1_g000022 Rmu_sc0005038.1_g000023 Rmu_sc0005840.1_g000014 Rmu_sc0006724.1_g000003 Rmu_sc0006824.1_g000005 Rmu_sc0007869.1_g000003 Rmu_sc0007869.1_g000005 Rmu_sc0007869.1_g000008 Rmu_sc0008646.1_g000007 Rmu_sc0009637.1_g000009 Rmu_sc0010500.1_g000002 Rmu_sc0010500.1_g000012 Rmu_sc0012403.1_g000025 Rmu_sc0013768.1_g000025 Rmu_sc0015625.1_g000001 Rmu_sc0019715.1_g000001 Rmu_sc0021133.1_g000002 Rmu_sc0021904.1_g000001 Rmu_sc0021904.1_g000003 Rmu_sc0021904.1_g000004 Rmu_sc0022054.1_g000001 Rmu_sc0026025.1_g000001 Rmu_sc0036828.1_g000002 Rmu_sc0041808.1_g000001 Rmu_ssc0000012.1_g000001
rosa_roxburghii Rroxscaffold_3G00226370 Rroxscaffold_3G00226860 Rroxscaffold_3G00226900 Rroxscaffold_3G00226930 Rroxscaffold_3G00226940 Rroxscaffold_3G00226970 Rroxscaffold_3G00226980 Rroxscaffold_3G00231530 Rroxscaffold_3G00237090 Rroxscaffold_3G00250760 Rroxscaffold_3G00250800
rosa_rugosa Rorug04G0082300 Rorug07G0101800 Rorug07G0102000 Rorug07G0102100 Rorug07G0221200 Rorug07G0245700 Rorug07G0245700 Rorug07G0245700 Rorug07G0263900 Rorug07G0264000 Rorug07G0264000 Rorug07G0264500 Rorug07G0282900 Rorug07G0282900 Rorug07G0283100 Rorug07G0283200.1 Rorug07G0283300 Rorug07G0283900 Rorug07G0284000 Rorug07G0284100 Rorug07G0284300 Rorug07G0284400 Rorug07G0284500 Rorug07G0285000 Rorug07G0285200 Rorug07G0285300 Rorug07G0285500 Rorug07G0286800 Rorug07G0286900 Rorug07G0287100 Rorug07G0287200
rosa_samantha Rh7AG236100 Rh7AG236200 Rh7AG236300 Rh7AG440000 Rh7AG440100 Rh7BG411900 Rh7BG412000 Rh7BG412600 Rh7CG251300 Rh7CG417700 Rh7CG460100 Rh7DG242100 Rh7DG242200 Rh7DG242300 Rh7DG429300
rosa_wichuraiana Rw0G005070 Rw0G010780 Rw0G012530 Rw7G020110 Rw7G020160 Rw7G020170 Rw7G033210 Rw7G033230 Rw7G033250 Rw7G034540 Rw7G035150 Rw7G036190 Rw7G036210 Rw7G036260 Rw7G036270 Rw7G036290 Rw7G036300 Rw7G036310 Rw7G036350 Rw7G036360 Rw7G036440 Rw7G036510 Rw7G036530 Rw7G036570 Rw7G036590 Rw7G036730

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 873
AccB7I CCANNNNNTGG 2 cut(s) 3576, 3748
AccI GTMKAC 2 cut(s) 84, 1071
AccII CGCG 2 cut(s) 119, 657
AccIII TCCGGA 1 cut(s) 3544
AciI CCGC 3 cut(s) 78, 119, 254
AclI AACGTT 2 cut(s) 918, 1159
AclWI GGATC 3 cut(s) 341, 805, 1376
AcoI YGGCCR 2 cut(s) 732, 1569
AcuI CTGAAG 3 cut(s) 1253, 1695, 1723
AcyI GRCGYC 1 cut(s) 531
AfaI GTAC 4 cut(s) 1744, 1941, 2268, 2301
AfiI CCNNNNNNNGG 6 cut(s) 118, 1277, 1340, 1639, 3576, 3748
AflII CTTAAG 1 cut(s) 1205
AflIII ACRYGT 3 cut(s) 60, 319, 355
AjiI CACGTC 1 cut(s) 61
AjnI CCWGG 6 cut(s) 141, 201, 1123, 1606, 2161, 2961
AjuI GAANNNNNNNTTGG 4 cut(s) 1096, 1128, 1295, 1327
Alw21I GWGCWC 2 cut(s) 1906, 3599
Alw26I GTCTC 2 cut(s) 297, 1768
Alw44I GTGCAC 1 cut(s) 3595
AlwI GGATC 3 cut(s) 341, 805, 1376
AlwNI CAGNNNCTG 1 cut(s) 491
Ama87I CYCGRG 1 cut(s) 2234
Aor13HI TCCGGA 1 cut(s) 3544
AoxI GGCC 6 cut(s) 106, 732, 1120, 1268, 1569, 2506
ApaLI GTGCAC 1 cut(s) 3595
ApeKI GCWGC 9 cut(s) 491, 611, 857, 1720, 1780, 1783, 1798, 2139, 2688
ArsI GACNNNNNNTTYG 2 cut(s) 516, 548
AseI ATTAAT 1 cut(s) 1577
Asp700I GAANNNNTTC 2 cut(s) 510, 1161
AspLEI GCGC 2 cut(s) 657, 716
AspS9I GGNCC 3 cut(s) 106, 1121, 3628
AsuC2I CCSGG 1 cut(s) 2505
AsuHPI GGTGA 7 cut(s) 295, 548, 1595, 2010, 2081, 2137, 3728
AsuII TTCGAA 2 cut(s) 1732, 3485
AvaI CYCGRG 1 cut(s) 2234
AvaII GGWCC 1 cut(s) 3628
BaeGI GKGCMC 1 cut(s) 3599
BaeI ACNNNNGTAYC 2 cut(s) 3310, 3343
BalI TGGCCA 2 cut(s) 734, 1571
BanII GRGCYC 1 cut(s) 1958
BarI GAAGNNNNNNTAC 2 cut(s) 1726, 1758
BauI CACGAG 1 cut(s) 1149
BbsI GAAGAC 3 cut(s) 227, 229, 958
Bbv12I GWGCWC 2 cut(s) 1906, 3599
BbvI GCAGC 9 cut(s) 478, 598, 844, 1707, 1767, 1795, 1810, 2126, 2675
BccI CCATC 6 cut(s) 639, 1425, 1457, 3633, 3707, 3742
BcgI CGANNNNNNTGC 4 cut(s) 1712, 1746, 3652, 3686
BciT130I CCWGG 6 cut(s) 143, 203, 1125, 1608, 2163, 2963
BciVI GTATCC 1 cut(s) 131
BclI TGATCA 1 cut(s) 1891
BcnI CCSGG 1 cut(s) 2505
BcoDI GTCTC 2 cut(s) 297, 1768
BfaI CTAG 5 cut(s) 1088, 1379, 2345, 2931, 3032
BfmI CTRYAG 9 cut(s) 1781, 2551, 2689, 2770, 2908, 2977, 3046, 3115, 3322
BfrI CTTAAG 1 cut(s) 1205
BfuI GTATCC 1 cut(s) 131
BglII AGATCT 1 cut(s) 2404
BmcAI AGTACT 1 cut(s) 2268
Bme1390I CCNGG 7 cut(s) 143, 203, 1125, 1608, 2163, 2505, 2963
Bme18I GGWCC 1 cut(s) 3628
BmeT110I CYCGRG 1 cut(s) 2234
BmgBI CACGTC 1 cut(s) 61
BmgT120I GGNCC 3 cut(s) 106, 1121, 3628
BmiI GGNNCC 2 cut(s) 1631, 1957
BmrFI CCNGG 7 cut(s) 143, 203, 1125, 1608, 2163, 2505, 2963
BmrI ACTGGG 2 cut(s) 320, 737
BmsI GCATC 6 cut(s) 1081, 1502, 1847, 2285, 3433, 3664
BmuI ACTGGG 2 cut(s) 320, 737
BpiI GAAGAC 3 cut(s) 227, 229, 958
BpmI CTGGAG 7 cut(s) 185, 1629, 1728, 2558, 2765, 2984, 3329
Bpu14I TTCGAA 2 cut(s) 1732, 3485
BpuEI CTTGAG 1 cut(s) 88
BpuMI CCSGG 1 cut(s) 2505
BsaHI GRCGYC 1 cut(s) 531
BsaJI CCNNGG 5 cut(s) 1123, 1606, 2162, 2235, 2961
BsaWI WCCGGW 1 cut(s) 3544
BsaXI ACNNNNNCTCC 3 cut(s) 32, 2687, 2717
Bsc4I CCNNNNNNNGG 6 cut(s) 118, 1277, 1340, 1639, 3576, 3748
Bse1I ACTGG 5 cut(s) 315, 743, 996, 1911, 3523
Bse3DI GCAATG 2 cut(s) 100, 2617
BseAI TCCGGA 1 cut(s) 3544
BseBI CCWGG 6 cut(s) 143, 203, 1125, 1608, 2163, 2963
BseDI CCNNGG 5 cut(s) 1123, 1606, 2162, 2235, 2961
BseLI CCNNNNNNNGG 6 cut(s) 118, 1277, 1340, 1639, 3576, 3748
BseMI GCAATG 2 cut(s) 100, 2617
BseMII CTCAG 4 cut(s) 255, 1763, 2005, 2501
BseNI ACTGG 5 cut(s) 315, 743, 996, 1911, 3523
BseRI GAGGAG 2 cut(s) 10, 22
BseSI GKGCMC 1 cut(s) 3599
BseXI GCAGC 9 cut(s) 478, 598, 844, 1707, 1767, 1795, 1810, 2126, 2675
BsgI GTGCAG 2 cut(s) 319, 1817
Bsh1236I CGCG 2 cut(s) 119, 657
BshFI GGCC 6 cut(s) 108, 734, 1122, 1270, 1571, 2508
BsiHKAI GWGCWC 2 cut(s) 1906, 3599
BsiHKCI CYCGRG 1 cut(s) 2234
BsiSI CCGG 2 cut(s) 2505, 3545
BslI CCNNNNNNNGG 6 cut(s) 118, 1277, 1340, 1639, 3576, 3748
BsmAI GTCTC 2 cut(s) 297, 1768
BsmI GAATGC 1 cut(s) 762
BsnI GGCC 6 cut(s) 108, 734, 1122, 1270, 1571, 2508
BsoBI CYCGRG 1 cut(s) 2234
Bsp119I TTCGAA 2 cut(s) 1732, 3485
Bsp1286I GDGCHC 3 cut(s) 1906, 1958, 3599
Bsp13I TCCGGA 1 cut(s) 3544
Bsp1407I TGTACA 1 cut(s) 1939
BspACI CCGC 3 cut(s) 78, 119, 254
BspANI GGCC 6 cut(s) 108, 734, 1122, 1270, 1571, 2508
BspCNI CTCAG 4 cut(s) 254, 1764, 2004, 2502
BspEI TCCGGA 1 cut(s) 3544
BspFNI CGCG 2 cut(s) 119, 657
BspLI GGNNCC 2 cut(s) 1631, 1957
BspMAI CTGCAG 9 cut(s) 1785, 2555, 2693, 2774, 2912, 2981, 3050, 3119, 3326
BspPI GGATC 3 cut(s) 341, 805, 1376
BspT104I TTCGAA 2 cut(s) 1732, 3485
BspTI CTTAAG 1 cut(s) 1205
BsrDI GCAATG 2 cut(s) 100, 2617
BsrGI TGTACA 1 cut(s) 1939
BsrI ACTGG 5 cut(s) 315, 743, 996, 1911, 3523
BssECI CCNNGG 5 cut(s) 1123, 1606, 2162, 2235, 2961
BssNI GRCGYC 1 cut(s) 531
BssSI CACGAG 1 cut(s) 1149
Bst2BI CACGAG 1 cut(s) 1149
Bst2UI CCWGG 6 cut(s) 143, 203, 1125, 1608, 2163, 2963
Bst4CI ACNGT 2 cut(s) 2075, 3185
Bst6I CTCTTC 4 cut(s) 28, 570, 2043, 2163
BstACI GRCGYC 1 cut(s) 531
BstAFI CTTAAG 1 cut(s) 1205
BstAPI GCANNNNNTGC 1 cut(s) 1555
BstAUI TGTACA 1 cut(s) 1939
BstBI TTCGAA 2 cut(s) 1732, 3485
BstC8I GCNNGC 3 cut(s) 1049, 1251, 3661
BstDEI CTNAG 6 cut(s) 241, 987, 1358, 1772, 1991, 2510
BstENI CCTNNNNNAGG 2 cut(s) 1275, 1338
BstFNI CGCG 2 cut(s) 119, 657
BstHHI GCGC 2 cut(s) 657, 716
BstMAI GTCTC 2 cut(s) 297, 1768
BstMWI GCNNNNNNNGC 5 cut(s) 105, 500, 863, 1555, 1726
BstNI CCWGG 6 cut(s) 143, 203, 1125, 1608, 2163, 2963
BstNSI RCATGY 3 cut(s) 323, 359, 1253
BstSCI CCNGG 7 cut(s) 141, 201, 1123, 1606, 2161, 2503, 2961
BstSFI CTRYAG 9 cut(s) 1781, 2551, 2689, 2770, 2908, 2977, 3046, 3115, 3322
BstSLI GKGCMC 1 cut(s) 3599
BstUI CGCG 2 cut(s) 119, 657
BstV1I GCAGC 9 cut(s) 478, 598, 844, 1707, 1767, 1795, 1810, 2126, 2675
BstV2I GAAGAC 3 cut(s) 227, 229, 958
BstX2I RGATCY 2 cut(s) 1381, 2404
BstYI RGATCY 2 cut(s) 1381, 2404
BsuI GTATCC 1 cut(s) 131
BsuRI GGCC 6 cut(s) 108, 734, 1122, 1270, 1571, 2508
BtrI CACGTC 1 cut(s) 61
BtsI GCAGTG 9 cut(s) 473, 593, 2548, 2767, 2905, 2974, 3043, 3112, 3319
Cac8I GCNNGC 3 cut(s) 1049, 1251, 3661
CaiI CAGNNNCTG 1 cut(s) 491
CfoI GCGC 2 cut(s) 657, 716
Cfr13I GGNCC 3 cut(s) 106, 1121, 3628
CseI GACGC 2 cut(s) 520, 2337
Csp6I GTAC 4 cut(s) 1743, 1940, 2267, 2300
CviAII CATG 8 cut(s) 292, 320, 356, 814, 1180, 1250, 1550, 3571
CviQI GTAC 4 cut(s) 1743, 1940, 2267, 2300
DdeI CTNAG 6 cut(s) 241, 987, 1358, 1772, 1991, 2510
DraI TTTAAA 1 cut(s) 1866
EaeI YGGCCR 2 cut(s) 732, 1569
Eam1104I CTCTTC 4 cut(s) 28, 570, 2043, 2163
EarI CTCTTC 4 cut(s) 28, 570, 2043, 2163
Eco24I GRGCYC 1 cut(s) 1958
Eco32I GATATC 2 cut(s) 772, 1283
Eco47I GGWCC 1 cut(s) 3628
Eco57I CTGAAG 3 cut(s) 1253, 1695, 1723
Eco88I CYCGRG 1 cut(s) 2234
EcoNI CCTNNNNNAGG 2 cut(s) 1275, 1338
EcoO109I RGGNCCY 1 cut(s) 3628
EcoRII CCWGG 6 cut(s) 141, 201, 1123, 1606, 2161, 2961
EcoRV GATATC 2 cut(s) 772, 1283
EcoT22I ATGCAT 1 cut(s) 1551
EcoT38I GRGCYC 1 cut(s) 1958
FaeI CATG 8 cut(s) 295, 323, 359, 817, 1183, 1253, 1553, 3574
FalI AAGNNNNNCTT 6 cut(s) 651, 683, 939, 971, 1269, 1301
FatI CATG 8 cut(s) 291, 319, 355, 813, 1179, 1249, 1549, 3570
FauNDI CATATG 1 cut(s) 802
FbaI TGATCA 1 cut(s) 1891
FblI GTMKAC 2 cut(s) 84, 1071
FriOI GRGCYC 1 cut(s) 1958
FspBI CTAG 5 cut(s) 1088, 1379, 2345, 2931, 3032
GlaI GCGC 2 cut(s) 656, 715
GsuI CTGGAG 7 cut(s) 185, 1629, 1728, 2558, 2765, 2984, 3329
HaeIII GGCC 6 cut(s) 108, 734, 1122, 1270, 1571, 2508
HapII CCGG 2 cut(s) 2505, 3545
HgaI GACGC 2 cut(s) 520, 2337
HhaI GCGC 2 cut(s) 657, 716
Hin1I GRCGYC 1 cut(s) 531
Hin1II CATG 8 cut(s) 295, 323, 359, 817, 1183, 1253, 1553, 3574
Hin6I GCGC 2 cut(s) 655, 714
HinP1I GCGC 2 cut(s) 655, 714
HincII GTYRAC 2 cut(s) 685, 3709
HindII GTYRAC 2 cut(s) 685, 3709
HindIII AAGCTT 6 cut(s) 665, 1366, 1735, 2663, 2732, 3365
HpaII CCGG 2 cut(s) 2505, 3545
HphI GGTGA 7 cut(s) 295, 548, 1595, 2010, 2081, 2137, 3728
Hpy188I TCNGA 9 cut(s) 244, 396, 711, 964, 1020, 1233, 1588, 2083, 2193
Hpy99I CGWCG 2 cut(s) 62, 536
HpyAV CCTTC 9 cut(s) 360, 521, 1271, 1334, 2501, 3212, 3365, 3619, 3757
HpyCH4III ACNGT 2 cut(s) 2075, 3185
HpyCH4IV ACGT 7 cut(s) 60, 439, 559, 918, 1039, 1159, 1794
HpyF10VI GCNNNNNNNGC 5 cut(s) 105, 500, 863, 1555, 1726
HpyF3I CTNAG 6 cut(s) 241, 987, 1358, 1772, 1991, 2510
HpySE526I ACGT 7 cut(s) 60, 439, 559, 918, 1039, 1159, 1794
Hsp92I GRCGYC 1 cut(s) 531
Hsp92II CATG 8 cut(s) 295, 323, 359, 817, 1183, 1253, 1553, 3574
HspAI GCGC 2 cut(s) 655, 714
Kpn2I TCCGGA 1 cut(s) 3544
Ksp22I TGATCA 1 cut(s) 1891
LmnI GCTCC 2 cut(s) 204, 1961
Lsp1109I GCAGC 9 cut(s) 478, 598, 844, 1707, 1767, 1795, 1810, 2126, 2675
LweI GCATC 6 cut(s) 1081, 1502, 1847, 2285, 3433, 3664
MaeI CTAG 5 cut(s) 1088, 1379, 2345, 2931, 3032
MaeII ACGT 7 cut(s) 60, 439, 559, 918, 1039, 1159, 1794
MflI RGATCY 2 cut(s) 1381, 2404
MhlI GDGCHC 3 cut(s) 1906, 1958, 3599
MlsI TGGCCA 2 cut(s) 734, 1571
MluNI TGGCCA 2 cut(s) 734, 1571
MlyI GAGTC 2 cut(s) 992, 2188
MmeI TCCRAC 4 cut(s) 727, 799, 1060, 1293
Mox20I TGGCCA 2 cut(s) 734, 1571
Mph1103I ATGCAT 1 cut(s) 1551
MroI TCCGGA 1 cut(s) 3544
MroXI GAANNNNTTC 2 cut(s) 510, 1161
MscI TGGCCA 2 cut(s) 734, 1571
MslI CAYNNNNRTG 2 cut(s) 405, 1184
Msp20I TGGCCA 2 cut(s) 734, 1571
MspA1I CMGCKG 2 cut(s) 857, 3611
MspCI CTTAAG 1 cut(s) 1205
MspI CCGG 2 cut(s) 2505, 3545
MspR9I CCNGG 7 cut(s) 143, 203, 1125, 1608, 2163, 2505, 2963
Mva1269I GAATGC 1 cut(s) 762
MvaI CCWGG 6 cut(s) 143, 203, 1125, 1608, 2163, 2963
MvnI CGCG 2 cut(s) 119, 657
MwoI GCNNNNNNNGC 5 cut(s) 105, 500, 863, 1555, 1726
NciI CCSGG 1 cut(s) 2505
NdeI CATATG 1 cut(s) 802
NlaIII CATG 8 cut(s) 295, 323, 359, 817, 1183, 1253, 1553, 3574
NlaIV GGNNCC 2 cut(s) 1631, 1957
NmuCI GTSAC 8 cut(s) 1601, 2765, 2903, 2972, 3041, 3110, 3248, 3317
NsiI ATGCAT 1 cut(s) 1551
NspI RCATGY 3 cut(s) 323, 359, 1253
NspV TTCGAA 2 cut(s) 1732, 3485
PaeI GCATGC 1 cut(s) 1253
PciI ACATGT 2 cut(s) 319, 355
PcsI WCGNNNNNNNCGW 2 cut(s) 528, 1156
PctI GAATGC 1 cut(s) 762
PdmI GAANNNNTTC 2 cut(s) 510, 1161
PfeI GAWTC 8 cut(s) 215, 257, 1491, 2086, 2146, 2326, 2357, 3541
PflFI GACNNNGTC 1 cut(s) 1445
PflMI CCANNNNNTGG 2 cut(s) 3576, 3748
PleI GAGTC 2 cut(s) 991, 2188
PpsI GAGTC 2 cut(s) 991, 2188
PpuMI RGGWCCY 1 cut(s) 3628
PscI ACATGT 2 cut(s) 319, 355
PshBI ATTAAT 1 cut(s) 1577
PsiI TTATAA 1 cut(s) 873
Psp1406I AACGTT 2 cut(s) 918, 1159
Psp5II RGGWCCY 1 cut(s) 3628
Psp6I CCWGG 6 cut(s) 141, 201, 1123, 1606, 2161, 2961
PspGI CCWGG 6 cut(s) 141, 201, 1123, 1606, 2161, 2961
PspN4I GGNNCC 2 cut(s) 1631, 1957
PspPI GGNCC 3 cut(s) 106, 1121, 3628
PspPPI RGGWCCY 1 cut(s) 3628
PstI CTGCAG 9 cut(s) 1785, 2555, 2693, 2774, 2912, 2981, 3050, 3119, 3326
PstNI CAGNNNCTG 1 cut(s) 491
PsuI RGATCY 2 cut(s) 1381, 2404
PsyI GACNNNGTC 1 cut(s) 1445
PvuII CAGCTG 2 cut(s) 857, 3611
RsaI GTAC 4 cut(s) 1744, 1941, 2268, 2301
RsaNI GTAC 4 cut(s) 1743, 1940, 2267, 2300
RseI CAYNNNNRTG 2 cut(s) 405, 1184
Sau96I GGNCC 3 cut(s) 106, 1121, 3628
ScaI AGTACT 1 cut(s) 2268
SchI GAGTC 2 cut(s) 992, 2188
ScrFI CCNGG 7 cut(s) 143, 203, 1125, 1608, 2163, 2505, 2963
SduI GDGCHC 3 cut(s) 1906, 1958, 3599
SfaNI GCATC 6 cut(s) 1081, 1502, 1847, 2285, 3433, 3664
SfcI CTRYAG 9 cut(s) 1781, 2551, 2689, 2770, 2908, 2977, 3046, 3115, 3322
SfuI TTCGAA 2 cut(s) 1732, 3485
SinI GGWCC 1 cut(s) 3628
SmiMI CAYNNNNRTG 2 cut(s) 405, 1184
SmlI CTYRAG 2 cut(s) 67, 1205
SmoI CTYRAG 2 cut(s) 67, 1205
SphI GCATGC 1 cut(s) 1253
SsiI CCGC 3 cut(s) 78, 119, 254
SspI AATATT 2 cut(s) 333, 1458
SspMI CTAG 5 cut(s) 1088, 1379, 2345, 2931, 3032
StyD4I CCNGG 7 cut(s) 141, 201, 1123, 1606, 2161, 2503, 2961
TaaI ACNGT 2 cut(s) 2075, 3185
TaiI ACGT 7 cut(s) 63, 442, 562, 921, 1042, 1162, 1797
TaqI TCGA 8 cut(s) 210, 218, 260, 1732, 2360, 3485, 3672, 3699
TatI WGTACW 3 cut(s) 1742, 1939, 2266
TauI GCSGC 1 cut(s) 122
TfiI GAWTC 8 cut(s) 215, 257, 1491, 2086, 2146, 2326, 2357, 3541
TseFI GTSAC 8 cut(s) 1601, 2765, 2903, 2972, 3041, 3110, 3248, 3317
TseI GCWGC 9 cut(s) 491, 611, 857, 1720, 1780, 1783, 1798, 2139, 2688
Tsp45I GTSAC 8 cut(s) 1601, 2765, 2903, 2972, 3041, 3110, 3248, 3317
TspGWI ACGGA 4 cut(s) 387, 2176, 2409, 3666
Tth111I GACNNNGTC 1 cut(s) 1445
Van91I CCANNNNNTGG 2 cut(s) 3576, 3748
Vha464I CTTAAG 1 cut(s) 1205
VneI GTGCAC 1 cut(s) 3595
VpaK11BI GGWCC 1 cut(s) 3628
VspI ATTAAT 1 cut(s) 1577
XagI CCTNNNNNAGG 2 cut(s) 1275, 1338
XceI RCATGY 3 cut(s) 323, 359, 1253
XmiI GTMKAC 2 cut(s) 84, 1071
XmnI GAANNNNTTC 2 cut(s) 510, 1161
XspI CTAG 5 cut(s) 1088, 1379, 2345, 2931, 3032
ZrmI AGTACT 1 cut(s) 2268
Zsp2I ATGCAT 1 cut(s) 1551
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.