Rmu_sc0000740.1_g000029

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000740.1
OG0000055
Physical Location & Seq
Reverse (-)
94858 .. 100740
5883 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0000740.1_g000029.1.cds

Sequence Viewer

Length: 3039 bp
atggcttcatcctacgacgtgttcttgagcttcagaggtccagacacacgcaacaacttcacgagccacctccacagcaaattagttgcgtctggaatccatcccttcatcgacgacgaagaactagagagaggggaagaaatatcaccgtcattgatcaggggaatcgaagggtgtaggatatctctcatagtcttctcagcgaactatgcgagttcaaaatggtgtcttgatgaggtggtgaagatcatggagtgtagaaaaacattggggcatactgtttttccaatattctatcgtgttgagccttcagatgtcaggcatcagacgggtagttttgcggaggcatttgtacaacataaagctgcaaatagatctgcagtgaaagtatccaggtggatagctgctcttcatgaagctgcaaatttgtctggcttccatcttagtactactgctaataggggtgaggcagggtttatcgatgacattgttgaccagattggtagacggctgaccaacacatacttacatgtagccgactacccaattggaatagattctcgcgttgaagagattagtaaatcattacgtgttggagcagatgatgatgttcacatggttggaatttggggtatgggtggaataggcaaaacatctgttgctaaagccatctataacaaattttatggtagttttgaaggtacaagttttcttgcaaatgttggggaaactgcgaagcagtcaaatggtaagatcactttgcaagaaacacttctgtctgatatcttaagaccaaccgagatagaagtaggtaatcctgatagagggatcattgtgataaaagaaagacttagaaagagaaaggtacttgtcattgttgatgatgtagacgatgtggaccaattaactgcattagccataaggcatgattcatttggtccaggaagtagaattgttataactacaagagatcaacatctgctagagcaacttaaagtgtcgatacatcttactcgtgaaatgaataaagaagaagctctggagctctttagttggcatgcatttcaaaattattgtcctgatgcaggatttttggagctctcaagaactgtggttacctactgtggaggtttaccactggctcttgaagttttaggttcttttctgtttggaaggaaggacgtaagagattggaaaagcacattgaagaaattggaaagaattcctgatggaaaaattcagaaaaagctcaaaataagctatgatgcaattgatgagaaccagaagaacttattccttgatgtatcttgtttcttcattggaatggacaagaaccatgtcataaatatactcgagggctgtggtctttctgcagcaatagataccagtgtcctcattcagcgttgccttttagttgttagtgaaaaaaacaaactcacaatgcacaatttgcttcgcgacatgggcagagaagttgttcgtgaacaatcccccgaagaccctgaagaacgtagtagattgtggtgtcaggaagatgtattagatatattgacagagggatctggaactaaagagattaaagggcttgctctgaatctgcaaagatctcagaaaaagactttcagaacacaagcatttagaaagatgaagaaactgaaattgctccaactcaactatcgcattcgctttgttgtcaagccaactcctatcaccgaaataacttcacatgaagttatttggcgggtaaatttgttaaacgatgagttcaatttggaaggtgatgactttgtgaaggttgaagttgcaattggatctggtttcagagtgaagaaaactggggtcagtcttgtatgggatcccaaacttatcaatgaaaatacgatcgagtgcgagcctatcccatacaaatactttccaagtgatgctgataatccggcaggaggctattactacaatataaagtcgtcctcctcatcgccgctgccatcatattcatcttcctcgcgacactggaagtacgaggtgttcttgagcttcaggggtgaagacacaggaaggaccttcacggaccatctctacgcggcactagatcgtgccggagttgtgacgtttcgggatgccgagggactaagaagtgaagaaaacatatcagaggaattggtgcggataatccaagagtgtaggatctctctcatcatcttctcagagaactacgcggattcaagttggtgtctcgaggagctggtgcagatcctggagtgtagaaaaacacggaggcagttggctttcccagtgttctattatgttgatccttctcatgtcagaaaccaggccggtagttttggtaaggcatttaagaatcatgaagctagagaaacggaagacaagatatccgggtggagagctgctctcacgggagcggcaaatttgactggctatgatacttctgtttgtcgggtcgaaggaagttgtattgaaataattgttgacaagatttgcgaaagcttaggcaaaggcatcttgttagtgcctcgcgaggcgattgcttcaaggctcattgagaaaaacaagctcatgatgcatgaattgcttcgagacatgggcagagaggttgttcctgaagaatcacctaactgtaactgccctaaaagacgtagtagattgtggcatccagtctgtcgaggtggtgactttgttgacgctgaagttgtaattgcttctggtttcactatgaagtatacaggggtcagtctagtatgggatcccaaacttatcaacgaaaataaaatagagtgcgagtcatcacttgataatgacttatttgatgatgactcatctgatgaggaccgatcttcagaaagattgaggtttgcgatgcaggcgcaacggcccatccatggaagaaaggttgaggtttgtgatgctggagcaacagcccatgagtctgcaaatacaccagctcaggagaggctcaagctcgcaagcaaaacccaaagtcaaatgtcccaatctttgcagttgttggtggtagttttctag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

1012

Amino Acids

114.14

Weight (kDa)

6.1

Isoelectric Point (pI)

46.55

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000055)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G36930 AT5G36930 AT5G36930 AT5G36930
fragaria_vesca FvH4_3g45361 FvH4_3g45380 FvH4_5g21061 FvH4_5g21062 FvH4_5g21063 FvH4_5g32050 FvH4_5g32970 FvH4_5g32990 FvH4_5g34190 FvH4_5g34210 FvH4_5g34210 FvH4_5g34210 FvH4_5g34210 FvH4_5g34210 FvH4_5g34210 FvH4_5g34211 FvH4_5g34240 FvH4_5g34250
malus_domestica MD00G1011700.v1.1 MD00G1026100.v1.1 MD00G1026500.v1.1 MD00G1027000.v1.1 MD00G1027100.v1.1 MD00G1027700.v1.1 MD00G1027800.v1.1 MD00G1028000.v1.1 MD00G1220000.v1.1 MD02G1024400.v1.1 MD02G1024800.v1.1 MD02G1026000.v1.1 MD02G1026100.v1.1 MD02G1031800.v1.1 MD02G1038500.v1.1 MD02G1038700.v1.1 MD02G1039200.v1.1 MD02G1040600.v1.1 MD02G1040900.v1.1 MD02G1041200.v1.1 MD02G1041300.v1.1 MD02G1041700.v1.1 MD02G1042000.v1.1 MD02G1043800.v1.1 MD02G1043900.v1.1 MD02G1044300.v1.1 MD02G1045200.v1.1 MD02G1045300.v1.1 MD02G1051200.v1.1 MD02G1052200.v1.1 MD02G1052800.v1.1 MD02G1053100.v1.1 MD02G1053600.v1.1 MD02G1055500.v1.1 MD02G1063300.v1.1 MD02G1063400.v1.1 MD02G1063800.v1.1 MD02G1306500.v1.1 MD02G1306600.v1.1 MD02G1306700.v1.1 MD02G1306900.v1.1 MD02G1307200.v1.1 MD04G1011700.v1.1 MD05G1315400.v1.1 MD05G1316000.v1.1 MD05G1316700.v1.1 MD05G1317000.v1.1 MD05G1317300.v1.1 MD05G1317600.v1.1 MD05G1317700.v1.1 MD07G1102800.v1.1 MD07G1238200.v1.1 MD08G1175900.v1.1 MD08G1203600.v1.1 MD09G1024200.v1.1 MD09G1039600.v1.1 MD09G1044800.v1.1 MD09G1045100.v1.1 MD10G1038700.v1.1 MD10G1039000.v1.1 MD10G1039200.v1.1 MD10G1040000.v1.1 MD10G1040200.v1.1 MD10G1040600.v1.1 MD10G1040800.v1.1 MD10G1297300.v1.1 MD12G1247500.v1.1 MD12G1247900.v1.1 MD12G1248300.v1.1 MD12G1248800.v1.1 MD15G1103700.v1.1 MD15G1103800.v1.1 MD15G1104000.v1.1 MD15G1179600.v1.1 MD15G1182500.v1.1 MD16G1068200.v1.1 MD16G1068300.v1.1 MD17G1024600.v1.1 MD17G1024700.v1.1 MD17G1025000.v1.1 MD17G1025900.v1.1
prunus_persica Prupe.1G539800_v2.0.a1 Prupe.1G550800_v2.0.a1 Prupe.1G550800_v2.0.a1 Prupe.1G550800_v2.0.a1 Prupe.1G550800_v2.0.a1 Prupe.1G550800_v2.0.a1 Prupe.1G550800_v2.0.a1 Prupe.1G557100_v2.0.a1 Prupe.1G557200_v2.0.a1 Prupe.1G557200_v2.0.a1 Prupe.1G557300_v2.0.a1
pyrus_communis pycom02g02080 pycom02g02090 pycom02g02110 pycom02g03220 pycom02g03260 pycom02g03370 pycom02g03390 pycom02g03410 pycom02g03420 pycom02g03450 pycom02g03500 pycom02g03730 pycom02g03770 pycom02g04400 pycom02g04410 pycom02g04430 pycom02g04450 pycom02g04460 pycom02g04490 pycom02g05200 pycom02g25710 pycom02g25750 pycom02g25790 pycom02g25810 pycom02g25830 pycom04g00920 pycom04g00930 pycom04g00940 pycom05g29300 pycom05g29310 pycom05g29380 pycom05g29410 pycom07g06310 pycom07g21420 pycom08g15070 pycom08g17540 pycom08g17560 pycom08g17570 pycom10g02670 pycom10g02680 pycom10g02700 pycom10g02770 pycom10g02790 pycom10g02800 pycom10g02810 pycom10g25020 pycom10g25030 pycom10g25050 pycom10g25080 pycom111g01990 pycom111g02000 pycom111g02030 pycom111g02040 pycom111g02050 pycom111g02060 pycom12g22710 pycom12g22720 pycom12g22730 pycom12g22740 pycom12g22750 pycom12g22770 pycom12g22780 pycom12g22790 pycom12g22800 pycom12g22810 pycom12g22830 pycom12g22840 pycom12g22860 pycom15g09440 pycom15g09480 pycom15g16120 pycom15g32380 pycom16g05970 pycom17g02100
rosa_chinensis RchiOBHm_Chr2g0144661 RchiOBHm_Chr4g0407491 RchiOBHm_Chr4g0407501 RchiOBHm_Chr4g0412381 RchiOBHm_Chr7g0208191 RchiOBHm_Chr7g0208201 RchiOBHm_Chr7g0208211 RchiOBHm_Chr7g0208221 RchiOBHm_Chr7g0228071 RchiOBHm_Chr7g0228121 RchiOBHm_Chr7g0230971 RchiOBHm_Chr7g0230981 RchiOBHm_Chr7g0233901 RchiOBHm_Chr7g0233951 RchiOBHm_Chr7g0233961 RchiOBHm_Chr7g0233991 RchiOBHm_Chr7g0234021 RchiOBHm_Chr7g0234151 RchiOBHm_Chr7g0234161 RchiOBHm_Chr7g0234241 RchiOBHm_Chr7g0234501 RchiOBHm_Chr7g0234641 RchiOBHm_Chr7g0234771 RchiOBHm_Chr7g0234781 RchiOBHm_Chr7g0234991 RchiOBHm_Chr7g0241291 RchiOBHm_Chr7g0241301
rosa_laevigata RLG00000001186 RLG00000001187 RLG00000001212 RLG00000001226 RLG00000001233 RLG00000001590 RLG00000003242 RLG00000003243 RLG00000003249 RLG00000008688 RLG00000008690
rosa_multiflora Rmu_co7966574.1_g000001 Rmu_co8120830.1_g000001 Rmu_co8142226.1_g000001 Rmu_co8293083.1_g000001 Rmu_co8351227.1_g000001 Rmu_co8380159.1_g000001 Rmu_co8456505.1_g000001 Rmu_sc0000676.1_g000025 Rmu_sc0000706.1_g000018 Rmu_sc0000706.1_g000040 Rmu_sc0000740.1_g000029 Rmu_sc0000740.1_g000032 Rmu_sc0000740.1_g000037 Rmu_sc0000740.1_g000040 Rmu_sc0000762.1_g000003 Rmu_sc0000762.1_g000019 Rmu_sc0000762.1_g000034 Rmu_sc0000762.1_g000069 Rmu_sc0000892.1_g000001 Rmu_sc0000945.1_g000040 Rmu_sc0001913.1_g000037 Rmu_sc0001913.1_g000041 Rmu_sc0001913.1_g000049 Rmu_sc0002775.1_g000017 Rmu_sc0002775.1_g000018 Rmu_sc0002775.1_g000020 Rmu_sc0002775.1_g000021 Rmu_sc0002775.1_g000022 Rmu_sc0002775.1_g000025 Rmu_sc0002775.1_g000026 Rmu_sc0002775.1_g000028 Rmu_sc0002775.1_g000030 Rmu_sc0004858.1_g000002 Rmu_sc0004864.1_g000022 Rmu_sc0005038.1_g000023 Rmu_sc0005840.1_g000014 Rmu_sc0006724.1_g000003 Rmu_sc0006824.1_g000005 Rmu_sc0007869.1_g000003 Rmu_sc0007869.1_g000005 Rmu_sc0007869.1_g000008 Rmu_sc0008646.1_g000007 Rmu_sc0009637.1_g000009 Rmu_sc0010500.1_g000002 Rmu_sc0010500.1_g000012 Rmu_sc0012403.1_g000025 Rmu_sc0013768.1_g000025 Rmu_sc0015625.1_g000001 Rmu_sc0019715.1_g000001 Rmu_sc0021133.1_g000002 Rmu_sc0021904.1_g000001 Rmu_sc0021904.1_g000003 Rmu_sc0021904.1_g000004 Rmu_sc0022054.1_g000001 Rmu_sc0026025.1_g000001 Rmu_sc0036828.1_g000002 Rmu_sc0041808.1_g000001 Rmu_ssc0000012.1_g000001
rosa_roxburghii Rroxscaffold_3G00226370 Rroxscaffold_3G00226860 Rroxscaffold_3G00226900 Rroxscaffold_3G00226930 Rroxscaffold_3G00226940 Rroxscaffold_3G00226970 Rroxscaffold_3G00226980 Rroxscaffold_3G00231530 Rroxscaffold_3G00237090 Rroxscaffold_3G00250760 Rroxscaffold_3G00250800
rosa_rugosa Rorug04G0082300 Rorug07G0101800 Rorug07G0102000 Rorug07G0102100 Rorug07G0221200 Rorug07G0245700 Rorug07G0245700 Rorug07G0245700 Rorug07G0263900 Rorug07G0264000 Rorug07G0264000 Rorug07G0264500 Rorug07G0282900 Rorug07G0282900 Rorug07G0283100 Rorug07G0283200.1 Rorug07G0283300 Rorug07G0283900 Rorug07G0284000 Rorug07G0284100 Rorug07G0284300 Rorug07G0284400 Rorug07G0284500 Rorug07G0285000 Rorug07G0285200 Rorug07G0285300 Rorug07G0285500 Rorug07G0286800 Rorug07G0286900 Rorug07G0287100 Rorug07G0287200
rosa_samantha Rh7AG236100 Rh7AG236200 Rh7AG236300 Rh7AG440000 Rh7AG440100 Rh7BG411900 Rh7BG412000 Rh7BG412600 Rh7CG251300 Rh7CG417700 Rh7CG460100 Rh7DG242100 Rh7DG242200 Rh7DG242300 Rh7DG429300
rosa_wichuraiana Rw0G005070 Rw0G010780 Rw0G012530 Rw7G020110 Rw7G020160 Rw7G020170 Rw7G033210 Rw7G033230 Rw7G033250 Rw7G034540 Rw7G035150 Rw7G036190 Rw7G036210 Rw7G036260 Rw7G036270 Rw7G036290 Rw7G036300 Rw7G036310 Rw7G036350 Rw7G036360 Rw7G036440 Rw7G036510 Rw7G036530 Rw7G036570 Rw7G036590 Rw7G036730

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 959
AccBSI CCGCTC 1 cut(s) 2420
AccI GTMKAC 3 cut(s) 505, 888, 2738
AccII CGCG 6 cut(s) 564, 1458, 2005, 2081, 2216, 2535
AciI CCGC 7 cut(s) 341, 1741, 1979, 2081, 2164, 2216, 2420
AcsI RAATTY 7 cut(s) 424, 624, 680, 1221, 1236, 1747, 2425
AcuI CTGAAG 7 cut(s) 16, 294, 1524, 2020, 2640, 2724, 2838
AfaI GTAC 5 cut(s) 354, 448, 703, 867, 2018
AfiI CCNNNNNNNGG 4 cut(s) 824, 1085, 1940, 2897
AflII CTTAAG 1 cut(s) 787
AflIII ACRYGT 3 cut(s) 18, 529, 589
AjiI CACGTC 1 cut(s) 19
AjnI CCWGG 4 cut(s) 392, 940, 2253, 2328
AjuI GAANNNNNNNTTGG 2 cut(s) 1791, 1823
Alw21I GWGCWC 2 cut(s) 1047, 1101
Alw26I GTCTC 2 cut(s) 2237, 2589
AlwNI CAGNNNCTG 1 cut(s) 2254
Ama87I CYCGRG 2 cut(s) 1352, 2234
AoxI GGCC 2 cut(s) 2331, 2888
ApeKI GCWGC 6 cut(s) 365, 404, 419, 1373, 1981, 2405
ApoI RAATTY 7 cut(s) 424, 624, 680, 1221, 1236, 1747, 2425
ArsI GACNNNNNNTTYG 2 cut(s) 1060, 1092
Asp700I GAANNNNTTC 6 cut(s) 556, 771, 1221, 1292, 1476, 2589
AspLEI GCGC 1 cut(s) 2884
AspS9I GGNCC 7 cut(s) 38, 898, 938, 2058, 2068, 2845, 2889
AsuC2I CCSGG 1 cut(s) 2395
AsuHPI GGTGA 8 cut(s) 138, 253, 476, 1702, 1790, 2054, 2619, 2699
AvaI CYCGRG 2 cut(s) 1352, 2234
AvaII GGWCC 6 cut(s) 38, 898, 938, 2058, 2068, 2845
BaeI ACNNNNGTAYC 4 cut(s) 1374, 1407, 2433, 2466
BamHI GGATCC 2 cut(s) 1855, 2761
BanII GRGCYC 2 cut(s) 1047, 1101
BauI CACGAG 2 cut(s) 61, 1014
BbsI GAAGAC 4 cut(s) 187, 1503, 2052, 2388
Bbv12I GWGCWC 2 cut(s) 1047, 1101
BbvI GCAGC 6 cut(s) 352, 391, 406, 1385, 1968, 2392
BccI CCATC 7 cut(s) 108, 447, 677, 1223, 1993, 2079, 2900
BceAI ACGGC 2 cut(s) 524, 2903
BcgI CGANNNNNNTGC 2 cut(s) 2525, 2559
BciT130I CCWGG 4 cut(s) 394, 942, 2255, 2330
BciVI GTATCC 1 cut(s) 400
BclI TGATCA 1 cut(s) 156
BcnI CCSGG 1 cut(s) 2395
BcoDI GTCTC 2 cut(s) 2237, 2589
BfaI CTAG 6 cut(s) 125, 983, 2087, 2370, 2753, 3037
BfmI CTRYAG 2 cut(s) 378, 1371
BfrI CTTAAG 1 cut(s) 787
BfuI GTATCC 1 cut(s) 400
BglII AGATCT 2 cut(s) 374, 1604
BisI GCNGC 9 cut(s) 366, 405, 420, 1374, 1979, 1982, 2082, 2406, 2421
BlsI GCNGC 9 cut(s) 367, 406, 421, 1375, 1980, 1983, 2083, 2407, 2422
BmcAI AGTACT 1 cut(s) 448
Bme1390I CCNGG 5 cut(s) 394, 942, 2255, 2330, 2395
Bme18I GGWCC 6 cut(s) 38, 898, 938, 2058, 2068, 2845
BmeT110I CYCGRG 2 cut(s) 1352, 2234
BmgBI CACGTC 1 cut(s) 19
BmgT120I GGNCC 7 cut(s) 38, 898, 938, 2058, 2068, 2845, 2889
BmiI GGNNCC 2 cut(s) 1857, 2763
BmrFI CCNGG 5 cut(s) 394, 942, 2255, 2330, 2395
BmrI ACTGGG 2 cut(s) 1845, 2285
BmuI ACTGGG 2 cut(s) 1845, 2285
BpiI GAAGAC 4 cut(s) 187, 1503, 2052, 2388
BplI GAGNNNNNCTC 4 cut(s) 2392, 2424, 2394, 2426
BpmI CTGGAG 3 cut(s) 1061, 2276, 2946
Bpu10I CCTNAGC 2 cut(s) 2506, 2961
BpuEI CTTGAG 4 cut(s) 46, 1087, 2050, 2957
BpuMI CCSGG 1 cut(s) 2395
Bsa29I ATCGAT 1 cut(s) 480
BsaAI YACGTR 1 cut(s) 590
BsaBI GATNNNNATC 1 cut(s) 975
BsaJI CCNNGG 2 cut(s) 2121, 2896
Bsc4I CCNNNNNNNGG 4 cut(s) 824, 1085, 1940, 2897
Bse118I RCCGGY 1 cut(s) 2333
Bse1I ACTGG 7 cut(s) 1143, 1386, 1840, 2015, 2291, 2437, 2672
Bse8I GATNNNNATC 1 cut(s) 975
BseBI CCWGG 4 cut(s) 394, 942, 2255, 2330
BseCI ATCGAT 1 cut(s) 480
BseDI CCNNGG 2 cut(s) 2121, 2896
BseGI GGATG 5 cut(s) 8, 100, 2122, 2668, 2892
BseJI GATNNNNATC 1 cut(s) 975
BseLI CCNNNNNNNGG 4 cut(s) 824, 1085, 1940, 2897
BseMII CTCAG 4 cut(s) 213, 1622, 2217, 2975
BseNI ACTGG 7 cut(s) 1143, 1386, 1840, 2015, 2291, 2437, 2672
BseRI GAGGAG 2 cut(s) 1960, 2252
BseXI GCAGC 6 cut(s) 352, 391, 406, 1385, 1968, 2392
BsgI GTGCAG 1 cut(s) 2267
Bsh1236I CGCG 6 cut(s) 564, 1458, 2005, 2081, 2216, 2535
Bsh1285I CGRYCG 1 cut(s) 1884
BshFI GGCC 2 cut(s) 2333, 2890
BshVI ATCGAT 1 cut(s) 480
BsiEI CGRYCG 1 cut(s) 1884
BsiHKAI GWGCWC 2 cut(s) 1047, 1101
BsiHKCI CYCGRG 2 cut(s) 1352, 2234
BsiSI CCGG 4 cut(s) 1934, 2097, 2334, 2394
BslFI GGGAC 2 cut(s) 2139, 2989
BslI CCNNNNNNNGG 4 cut(s) 824, 1085, 1940, 2897
BsmAI GTCTC 2 cut(s) 2237, 2589
BsmFI GGGAC 2 cut(s) 2139, 2989
BsmI GAATGC 1 cut(s) 1680
BsnI GGCC 2 cut(s) 2333, 2890
BsoBI CYCGRG 2 cut(s) 1352, 2234
Bsp1286I GDGCHC 2 cut(s) 1047, 1101
Bsp1407I TGTACA 1 cut(s) 352
Bsp19I CCATGG 1 cut(s) 2896
Bsp68I TCGCGA 3 cut(s) 1458, 2005, 2535
BspACI CCGC 7 cut(s) 341, 1741, 1979, 2081, 2164, 2216, 2420
BspANI GGCC 2 cut(s) 2333, 2890
BspCNI CTCAG 4 cut(s) 212, 1621, 2216, 2974
BspDI ATCGAT 1 cut(s) 480
BspFNI CGCG 6 cut(s) 564, 1458, 2005, 2081, 2216, 2535
BspHI TCATGA 3 cut(s) 412, 2362, 2574
BspLI GGNNCC 2 cut(s) 1857, 2763
BspMAI CTGCAG 2 cut(s) 382, 1375
BspQI GCTCTTC 1 cut(s) 414
BspTI CTTAAG 1 cut(s) 787
BsrBI CCGCTC 1 cut(s) 2420
BsrFI RCCGGY 1 cut(s) 2333
BsrGI TGTACA 1 cut(s) 352
BsrI ACTGG 7 cut(s) 1143, 1386, 1840, 2015, 2291, 2437, 2672
BssAI RCCGGY 1 cut(s) 2333
BssECI CCNNGG 2 cut(s) 2121, 2896
BssNAI GTATAC 1 cut(s) 2739
BssSI CACGAG 2 cut(s) 61, 1014
BssT1I CCWWGG 1 cut(s) 2896
Bst1107I GTATAC 1 cut(s) 2739
Bst2BI CACGAG 2 cut(s) 61, 1014
Bst2UI CCWGG 4 cut(s) 394, 942, 2255, 2330
Bst4CI ACNGT 5 cut(s) 150, 280, 1111, 1124, 2636
Bst6I CTCTTC 2 cut(s) 414, 564
BstAFI CTTAAG 1 cut(s) 787
BstAPI GCANNNNNTGC 2 cut(s) 1450, 2587
BstAUI TGTACA 1 cut(s) 352
BstBAI YACGTR 1 cut(s) 590
BstC8I GCNNGC 6 cut(s) 1059, 1587, 1892, 2880, 2979, 2983
BstDEI CTNAG 8 cut(s) 199, 443, 851, 1608, 2129, 2203, 2506, 2961
BstDSI CCRYGG 1 cut(s) 2896
BstEII GGTNACC 1 cut(s) 1114
BstENI CCTNNNNNAGG 2 cut(s) 822, 1083
BstF5I GGATG 5 cut(s) 8, 100, 2122, 2668, 2892
BstFNI CGCG 6 cut(s) 564, 1458, 2005, 2081, 2216, 2535
BstHHI GCGC 1 cut(s) 2884
BstMAI GTCTC 2 cut(s) 2237, 2589
BstMCI CGRYCG 1 cut(s) 1884
BstMWI GCNNNNNNNGC 6 cut(s) 209, 1450, 1464, 2578, 2587, 2879
BstNI CCWGG 4 cut(s) 394, 942, 2255, 2330
BstNSI RCATGY 2 cut(s) 533, 1061
BstPI GGTNACC 1 cut(s) 1114
BstSCI CCNGG 5 cut(s) 392, 940, 2253, 2328, 2393
BstSFI CTRYAG 2 cut(s) 378, 1371
BstUI CGCG 6 cut(s) 564, 1458, 2005, 2081, 2216, 2535
BstV1I GCAGC 6 cut(s) 352, 391, 406, 1385, 1968, 2392
BstV2I GAAGAC 4 cut(s) 187, 1503, 2052, 2388
BstX2I RGATCY 8 cut(s) 374, 1559, 1604, 1811, 1855, 2184, 2250, 2761
BstYI RGATCY 8 cut(s) 374, 1559, 1604, 1811, 1855, 2184, 2250, 2761
BstZ17I GTATAC 1 cut(s) 2739
Bsu15I ATCGAT 1 cut(s) 480
BsuI GTATCC 1 cut(s) 400
BsuRI GGCC 2 cut(s) 2333, 2890
BsuTUI ATCGAT 1 cut(s) 480
BtgI CCRYGG 1 cut(s) 2896
BtgZI GCGATG 2 cut(s) 1959, 2888
BtrI CACGTC 1 cut(s) 19
BtsCI GGATG 5 cut(s) 8, 100, 2122, 2668, 2892
BtsI GCAGTG 1 cut(s) 387
BtsIMutI CAGTG 5 cut(s) 387, 1136, 1393, 2008, 2298
BtuMI TCGCGA 3 cut(s) 1458, 2005, 2535
Cac8I GCNNGC 6 cut(s) 1059, 1587, 1892, 2880, 2979, 2983
CaiI CAGNNNCTG 1 cut(s) 2254
CciI TCATGA 3 cut(s) 412, 2362, 2574
CfoI GCGC 1 cut(s) 2884
Cfr10I RCCGGY 1 cut(s) 2333
Cfr13I GGNCC 7 cut(s) 38, 898, 938, 2058, 2068, 2845, 2889
ClaI ATCGAT 1 cut(s) 480
CseI GACGC 2 cut(s) 78, 2708
Csp6I GTAC 5 cut(s) 353, 447, 702, 866, 2017
CspCI CAANNNNNGTGG 2 cut(s) 1092, 1127
CviQI GTAC 5 cut(s) 353, 447, 702, 866, 2017
DdeI CTNAG 8 cut(s) 199, 443, 851, 1608, 2129, 2203, 2506, 2961
Eam1104I CTCTTC 2 cut(s) 414, 564
EarI CTCTTC 2 cut(s) 414, 564
Ecl136II GAGCTC 2 cut(s) 1045, 1099
Eco130I CCWWGG 1 cut(s) 2896
Eco24I GRGCYC 2 cut(s) 1047, 1101
Eco32I GATATC 3 cut(s) 183, 784, 2391
Eco47I GGWCC 6 cut(s) 38, 898, 938, 2058, 2068, 2845
Eco53kI GAGCTC 2 cut(s) 1045, 1099
Eco57I CTGAAG 7 cut(s) 16, 294, 1524, 2020, 2640, 2724, 2838
Eco88I CYCGRG 2 cut(s) 1352, 2234
Eco91I GGTNACC 1 cut(s) 1114
EcoICRI GAGCTC 2 cut(s) 1045, 1099
EcoNI CCTNNNNNAGG 2 cut(s) 822, 1083
EcoO109I RGGNCCY 1 cut(s) 2058
EcoO65I GGTNACC 1 cut(s) 1114
EcoRI GAATTC 1 cut(s) 1221
EcoRII CCWGG 4 cut(s) 392, 940, 2253, 2328
EcoRV GATATC 3 cut(s) 183, 784, 2391
EcoT14I CCWWGG 1 cut(s) 2896
EcoT22I ATGCAT 2 cut(s) 1063, 2583
EcoT38I GRGCYC 2 cut(s) 1047, 1101
ErhI CCWWGG 1 cut(s) 2896
FalI AAGNNNNNCTT 4 cut(s) 756, 788, 834, 866
FaqI GGGAC 2 cut(s) 2139, 2989
FauI CCCGC 1 cut(s) 1734
FbaI TGATCA 1 cut(s) 156
FblI GTMKAC 3 cut(s) 505, 888, 2738
Fnu4HI GCNGC 9 cut(s) 366, 405, 420, 1374, 1979, 1982, 2082, 2406, 2421
FokI GGATG 4 cut(s) 87, 2129, 2655, 2879
FriOI GRGCYC 2 cut(s) 1047, 1101
Fsp4HI GCNGC 9 cut(s) 366, 405, 420, 1374, 1979, 1982, 2082, 2406, 2421
FspBI CTAG 6 cut(s) 125, 983, 2087, 2370, 2753, 3037
GlaI GCGC 1 cut(s) 2883
GluI GCNGC 9 cut(s) 366, 405, 420, 1374, 1979, 1982, 2082, 2406, 2421
GsuI CTGGAG 3 cut(s) 1061, 2276, 2946
HaeIII GGCC 2 cut(s) 2333, 2890
HapII CCGG 4 cut(s) 1934, 2097, 2334, 2394
HgaI GACGC 2 cut(s) 78, 2708
HhaI GCGC 1 cut(s) 2884
Hin6I GCGC 1 cut(s) 2882
HinP1I GCGC 1 cut(s) 2882
HincII GTYRAC 3 cut(s) 493, 2488, 2698
HindII GTYRAC 3 cut(s) 493, 2488, 2698
HindIII AAGCTT 1 cut(s) 2503
HpaII CCGG 4 cut(s) 1934, 2097, 2334, 2394
HphI GGTGA 8 cut(s) 138, 253, 476, 1702, 1790, 2054, 2619, 2699
Hpy99I CGWCG 3 cut(s) 20, 116, 119
HpyCH4III ACNGT 5 cut(s) 150, 280, 1111, 1124, 2636
HpyCH4IV ACGT 6 cut(s) 18, 589, 1182, 1510, 2108, 2653
HpyF10VI GCNNNNNNNGC 6 cut(s) 209, 1450, 1464, 2578, 2587, 2879
HpyF3I CTNAG 8 cut(s) 199, 443, 851, 1608, 2129, 2203, 2506, 2961
HpySE526I ACGT 6 cut(s) 18, 589, 1182, 1510, 2108, 2653
HspAI GCGC 1 cut(s) 2882
Ksp22I TGATCA 1 cut(s) 156
LguI GCTCTTC 1 cut(s) 414
LmnI GCTCC 7 cut(s) 596, 1042, 1096, 1668, 2239, 2417, 2927
Lsp1109I GCAGC 6 cut(s) 352, 391, 406, 1385, 1968, 2392
MaeI CTAG 6 cut(s) 125, 983, 2087, 2370, 2753, 3037
MaeII ACGT 6 cut(s) 18, 589, 1182, 1510, 2108, 2653
MaeIII GTNAC 4 cut(s) 1114, 2104, 2636, 2687
MbiI CCGCTC 1 cut(s) 2420
MfeI CAATTG 3 cut(s) 546, 1269, 1806
MflI RGATCY 8 cut(s) 374, 1559, 1604, 1811, 1855, 2184, 2250, 2761
MhlI GDGCHC 2 cut(s) 1047, 1101
MlyI GAGTC 3 cut(s) 2807, 2825, 2951
MmeI TCCRAC 3 cut(s) 574, 601, 1690
Mph1103I ATGCAT 2 cut(s) 1063, 2583
MroXI GAANNNNTTC 6 cut(s) 556, 771, 1221, 1292, 1476, 2589
MseI TTAA 6 cut(s) 788, 905, 993, 1578, 1754, 2355
MslI CAYNNNNRTG 1 cut(s) 1322
MspA1I CMGCKG 1 cut(s) 1981
MspCI CTTAAG 1 cut(s) 787
MspI CCGG 4 cut(s) 1934, 2097, 2334, 2394
MspR9I CCNGG 5 cut(s) 394, 942, 2255, 2330, 2395
MunI CAATTG 3 cut(s) 546, 1269, 1806
Mva1269I GAATGC 1 cut(s) 1680
MvaI CCWGG 4 cut(s) 394, 942, 2255, 2330
MvnI CGCG 6 cut(s) 564, 1458, 2005, 2081, 2216, 2535
MwoI GCNNNNNNNGC 6 cut(s) 209, 1450, 1464, 2578, 2587, 2879
NciI CCSGG 1 cut(s) 2395
NcoI CCATGG 1 cut(s) 2896
NlaIV GGNNCC 2 cut(s) 1857, 2763
NmeAIII GCCGAG 1 cut(s) 2146
NmuCI GTSAC 2 cut(s) 2104, 2687
NruI TCGCGA 3 cut(s) 1458, 2005, 2535
NsiI ATGCAT 2 cut(s) 1063, 2583
NspI RCATGY 2 cut(s) 533, 1061
PaeI GCATGC 1 cut(s) 1061
PaeR7I CTCGAG 2 cut(s) 1352, 2234
PagI TCATGA 3 cut(s) 412, 2362, 2574
PciI ACATGT 1 cut(s) 529
PciSI GCTCTTC 1 cut(s) 414
PctI GAATGC 1 cut(s) 1680
PdmI GAANNNNTTC 6 cut(s) 556, 771, 1221, 1292, 1476, 2589
PfeI GAWTC 8 cut(s) 96, 165, 557, 929, 1594, 2219, 2359, 2624
PfoI TCCNGGA 2 cut(s) 940, 2253
PkrI GCNGC 9 cut(s) 367, 406, 421, 1375, 1980, 1983, 2083, 2407, 2422
Ple19I CGATCG 1 cut(s) 1884
PleI GAGTC 3 cut(s) 2806, 2825, 2950
PpsI GAGTC 3 cut(s) 2806, 2825, 2950
Ppu21I YACGTR 1 cut(s) 590
PpuMI RGGWCCY 1 cut(s) 2058
PscI ACATGT 1 cut(s) 529
PsiI TTATAA 1 cut(s) 959
Psp124BI GAGCTC 2 cut(s) 1047, 1101
Psp5II RGGWCCY 1 cut(s) 2058
Psp6I CCWGG 4 cut(s) 392, 940, 2253, 2328
PspEI GGTNACC 1 cut(s) 1114
PspGI CCWGG 4 cut(s) 392, 940, 2253, 2328
PspN4I GGNNCC 2 cut(s) 1857, 2763
PspPI GGNCC 7 cut(s) 38, 898, 938, 2058, 2068, 2845, 2889
PspPPI RGGWCCY 1 cut(s) 2058
PspXI VCTCGAGB 1 cut(s) 1352
PsrI GAACNNNNNNTAC 4 cut(s) 1099, 1131, 2009, 2041
PstI CTGCAG 2 cut(s) 382, 1375
PstNI CAGNNNCTG 1 cut(s) 2254
PsuI RGATCY 8 cut(s) 374, 1559, 1604, 1811, 1855, 2184, 2250, 2761
PvuI CGATCG 1 cut(s) 1884
RruI TCGCGA 3 cut(s) 1458, 2005, 2535
RsaI GTAC 5 cut(s) 354, 448, 703, 867, 2018
RsaNI GTAC 5 cut(s) 353, 447, 702, 866, 2017
RseI CAYNNNNRTG 1 cut(s) 1322
SacI GAGCTC 2 cut(s) 1047, 1101
SapI GCTCTTC 1 cut(s) 414
SaqAI TTAA 6 cut(s) 788, 905, 993, 1578, 1754, 2355
SatI GCNGC 9 cut(s) 366, 405, 420, 1374, 1979, 1982, 2082, 2406, 2421
Sau96I GGNCC 7 cut(s) 38, 898, 938, 2058, 2068, 2845, 2889
ScaI AGTACT 1 cut(s) 448
SchI GAGTC 3 cut(s) 2807, 2825, 2951
ScrFI CCNGG 5 cut(s) 394, 942, 2255, 2330, 2395
SduI GDGCHC 2 cut(s) 1047, 1101
SfcI CTRYAG 2 cut(s) 378, 1371
Sfr274I CTCGAG 2 cut(s) 1352, 2234
SinI GGWCC 6 cut(s) 38, 898, 938, 2058, 2068, 2845
SlaI CTCGAG 2 cut(s) 1352, 2234
SmiMI CAYNNNNRTG 1 cut(s) 1322
SmlI CTYRAG 7 cut(s) 25, 787, 1102, 1352, 2029, 2234, 2972
SmoI CTYRAG 7 cut(s) 25, 787, 1102, 1352, 2029, 2234, 2972
SphI GCATGC 1 cut(s) 1061
SsiI CCGC 7 cut(s) 341, 1741, 1979, 2081, 2164, 2216, 2420
SspI AATATT 1 cut(s) 291
SspMI CTAG 6 cut(s) 125, 983, 2087, 2370, 2753, 3037
SstI GAGCTC 2 cut(s) 1047, 1101
StyD4I CCNGG 5 cut(s) 392, 940, 2253, 2328, 2393
StyI CCWWGG 1 cut(s) 2896
TaaI ACNGT 5 cut(s) 150, 280, 1111, 1124, 2636
TaiI ACGT 6 cut(s) 21, 592, 1185, 1513, 2111, 2656
TaqII GACCGA 1 cut(s) 2862
TatI WGTACW 2 cut(s) 352, 446
TauI GCSGC 3 cut(s) 1981, 2084, 2423
TfiI GAWTC 8 cut(s) 96, 165, 557, 929, 1594, 2219, 2359, 2624
Tru1I TTAA 6 cut(s) 788, 905, 993, 1578, 1754, 2355
Tru9I TTAA 6 cut(s) 788, 905, 993, 1578, 1754, 2355
TscAI CASTG 5 cut(s) 387, 1143, 1393, 2015, 2298
TseFI GTSAC 2 cut(s) 2104, 2687
TseI GCWGC 6 cut(s) 365, 404, 419, 1373, 1981, 2405
Tsp45I GTSAC 2 cut(s) 2104, 2687
TspGWI ACGGA 3 cut(s) 2081, 2287, 2393
TspRI CASTG 5 cut(s) 387, 1143, 1393, 2015, 2298
Vha464I CTTAAG 1 cut(s) 787
VpaK11BI GGWCC 6 cut(s) 38, 898, 938, 2058, 2068, 2845
XagI CCTNNNNNAGG 2 cut(s) 822, 1083
XapI RAATTY 7 cut(s) 424, 624, 680, 1221, 1236, 1747, 2425
XceI RCATGY 2 cut(s) 533, 1061
XhoI CTCGAG 2 cut(s) 1352, 2234
XmiI GTMKAC 3 cut(s) 505, 888, 2738
XmnI GAANNNNTTC 6 cut(s) 556, 771, 1221, 1292, 1476, 2589
XspI CTAG 6 cut(s) 125, 983, 2087, 2370, 2753, 3037
ZrmI AGTACT 1 cut(s) 448
Zsp2I ATGCAT 2 cut(s) 1063, 2583
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.