Rw7G036510

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0000055
Physical Location & Seq
Forward (+)
55241945 .. 55245042
3098 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G036510.1

Sequence Viewer

Length: 2550 bp
ATGAAGGCATCGTCATCATCCTCATCGCTTCCATCTGGGCCACCATCCTCATGGCCACCGGAGTTCCCTGACTGCACGTACGACGTGTTCTTGAGTTTCAGAGGTGAAGACACACGAAAGACCTTCACGGACCACCTCTACGTGGCATTAAATCGCGCCGGGGTCAGAGCGTTTAGGGATGCCGAGGGTCTAACAAGGGGAGAACACATAGAATTTCAATTGGTGCGGATAATCCAAGGGTGTAGGATCTCTCTCATCGTCTTCTCAGAAAATTACGCGGATTCGAGATGGTGTCTCCAGGAGCTGGCACACATCATGGAGTGTAGAAAAACACATAGGCAACGGGTTTTCCCCATATTCTATCATGTCGATCCTTCACATGTCAGAAACCAGACCGGTAGCTTTGCTCATGCGTTTGAGAGACATGAAGCCACAGTACATCAAGATAGAGATAAGATAGCTAGGTGGAGAGCTGCTCTTAGAGGAGCGGCGGATTTGGCTGGCTTCGATATTGCTGATAGGCATGAAGCAGAGTTTATCAATAGCATTGTTGACGAGATATGCAGACAGCTGACCACCCCATACTTGAATTTAGCTGTCCATCCAGTTGGAATAGATTCTCGTGTTAAAGATATTGGTGAATATTTACGTTTTAGATTAGATAATGCTGTTTACATGGTTGGAATTTGGGGTATGGGTGGAATCGGCAAAACAACAGTTGCCAAAGCCATCTATAACAAATTTTATCATGACTTTGACAGTAGAAGTTTCCTAGCAGATGTTAGGGAAACAGCAAAGGACTCAAGTGGTAAGATTGCACTGCAAGAAAGACTTCTTTCTGATGTCTTAAAACCAACCAAGATAAAGGTAGGTGATGTTTCCAATGGCATCAATGTGATCAAAGAACGACTTAGAAGCAAAAAGGTACTTGTCATCGTTGACAATGTCGATCGTCTGGAACAATTGAATGCATTGGCAATAAGCCGTGATTCCTTTGGTCTGGGAAGTATAATTATTATAACAACAAGAGATCGACATTTGTTACAACAAGTGAGAGTGGATAAAATACATCTGACACAAGAAATGAATGAAGTAGAAGCTCTTGAGCTCTTCAGTTGGCATGCCTTTCAAAATTATTCTCCTAAGGAAGACTATATCGAACTCTCAAGAAGAGTGGTTACTTACTGTGGAGGTTTACCGCTGGCTCTTGAAGTTTTAGGGTCTTTCCTCTTTGGAAGGAGCATAGGAGATTGGAGTAGCACACTGAAGAAATTGGAAAAATTTCCTGATGACAAAATTCAGTCAAAGCTCAGAATAAGCTTCGATGCACTTGAGGAGTGGCAGAGGCACATATTCCTCCACATATGTTGTTTCTTCATTGGAATGGACAAAAACCATGTCATAAAAATACTGGAAGGCTGTGGTCTTGCTGCAGGTGTAGGCATTAGTGTCCTCTCTGAACGCTGCCTCGTAACTGTTAATGAAAAAGACAAGGTAATGATGCATGATTTGCTTCGAGACATGGGCAGAGAAATTGTTCGTGAAGAATCCCCGAACCACCCTGAAAGACGTAGTAGATTGTGGCGTGAGGAAGATGTAATAGATGTTTTGAGATATGAATTTGGAACTGAAGAGACTGAAGGACTCGCTCTAAATTTCCAAAGATCTGGCAGCATGACTTTCAGTACAGAAGCATTTAGAAACATGAGGGAACTGAAATTGCTCCAACTCAAGAATGTACTACTCGCTGGAGCAGTCAACAAGCTTCCCAAAAAGTTAAGATGGCTCTGCTTGCGAGGATCCTCTCTTGGAACTGCGTTTCTGGGTACAAATGACCGGGTTTCTGGTTTCTGTAGACCCGTAGATGAGTTTCTGGATACAATTGACCTGGTTTCTGTGGACCTGCGGTACGTCAATGTCCGACGAGTTCGGGAGCATTTCTGGCGGTTTGGGAGATTGGAGATACTTAATCTCAGTCATTCACAGCGCCTAACAACATCACCAGACTTTACACAACTCCCAAATCTACAGTATTTGATCCTTAAATACTGTGTGAATTTGACAGAGATTCACAGGTCCATTGCACTTGTTTCTCAACTTGCCTTGCTAAATCTTAAAGGCTGCACTAAGCTCAAATACCTTCCGGAGGGTTTTTATACGTTGCGTTCTGTTAGGACTCTTGTTCTTTCTGGCTGTTCGAAATTTGAGAAATTGAGCAAGCATATAGGAAAAATGGGTTCTTTGAAAACTCTTGTTATAAGTGGCACAGCCATAAGTGAAGTACCATCCTCGGTAGATGAATTGCAATACCTGGACTTTTCATCTCGACAAGGCCTGAGTGGACTAAAACGATTTACCCCGGATGAGATATCAGGACCTAGCCAGGACGAGACATCAGGACCTAGCGAGGATGAGACATCACGACTTGATTTTCTATTCAGTCGAGCCACTAGCAGTGCCGGCCCTAAGCCTAGGCGAAGCAGGGCCAGGCCTAGGGCCTCAGGTTTTAGGGGGCCTCAAAAAAATTCTTTATGGAGGCCCTGCAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

849

Amino Acids

96.61

Weight (kDa)

8.96

Isoelectric Point (pI)

47.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 27 - 192 2.8e-49 TIR domain
TIR_2 PF13676 29 - 125 4.3e-15 TIR domain
NB-ARC PF00931 222 - 378 2.2e-18 NB-ARC domain
WHD_ROQ1 PF23282 446 - 517 8.1e-20 Disease resistance protein Roq1-like, winged-helix domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000055)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G36930 AT5G36930 AT5G36930 AT5G36930
fragaria_vesca FvH4_3g45361 FvH4_3g45380 FvH4_5g21061 FvH4_5g21062 FvH4_5g21063 FvH4_5g32050 FvH4_5g32970 FvH4_5g32990 FvH4_5g34190 FvH4_5g34210 FvH4_5g34210 FvH4_5g34210 FvH4_5g34210 FvH4_5g34210 FvH4_5g34210 FvH4_5g34211 FvH4_5g34240 FvH4_5g34250
malus_domestica MD00G1011700.v1.1 MD00G1026100.v1.1 MD00G1026500.v1.1 MD00G1027000.v1.1 MD00G1027100.v1.1 MD00G1027700.v1.1 MD00G1027800.v1.1 MD00G1028000.v1.1 MD00G1220000.v1.1 MD02G1024400.v1.1 MD02G1024800.v1.1 MD02G1026000.v1.1 MD02G1026100.v1.1 MD02G1031800.v1.1 MD02G1038500.v1.1 MD02G1038700.v1.1 MD02G1039200.v1.1 MD02G1040600.v1.1 MD02G1040900.v1.1 MD02G1041200.v1.1 MD02G1041300.v1.1 MD02G1041700.v1.1 MD02G1042000.v1.1 MD02G1043800.v1.1 MD02G1043900.v1.1 MD02G1044300.v1.1 MD02G1045200.v1.1 MD02G1045300.v1.1 MD02G1051200.v1.1 MD02G1052200.v1.1 MD02G1052800.v1.1 MD02G1053100.v1.1 MD02G1053600.v1.1 MD02G1055500.v1.1 MD02G1063300.v1.1 MD02G1063400.v1.1 MD02G1063800.v1.1 MD02G1306500.v1.1 MD02G1306600.v1.1 MD02G1306700.v1.1 MD02G1306900.v1.1 MD02G1307200.v1.1 MD04G1011700.v1.1 MD05G1315400.v1.1 MD05G1316000.v1.1 MD05G1316700.v1.1 MD05G1317000.v1.1 MD05G1317300.v1.1 MD05G1317600.v1.1 MD05G1317700.v1.1 MD07G1102800.v1.1 MD07G1238200.v1.1 MD08G1175900.v1.1 MD08G1203600.v1.1 MD09G1024200.v1.1 MD09G1039600.v1.1 MD09G1044800.v1.1 MD09G1045100.v1.1 MD10G1038700.v1.1 MD10G1039000.v1.1 MD10G1039200.v1.1 MD10G1040000.v1.1 MD10G1040200.v1.1 MD10G1040600.v1.1 MD10G1040800.v1.1 MD10G1297300.v1.1 MD12G1247500.v1.1 MD12G1247900.v1.1 MD12G1248300.v1.1 MD12G1248800.v1.1 MD15G1103700.v1.1 MD15G1103800.v1.1 MD15G1104000.v1.1 MD15G1179600.v1.1 MD15G1182500.v1.1 MD16G1068200.v1.1 MD16G1068300.v1.1 MD17G1024600.v1.1 MD17G1024700.v1.1 MD17G1025000.v1.1 MD17G1025900.v1.1
prunus_persica Prupe.1G539800_v2.0.a1 Prupe.1G550800_v2.0.a1 Prupe.1G550800_v2.0.a1 Prupe.1G550800_v2.0.a1 Prupe.1G550800_v2.0.a1 Prupe.1G550800_v2.0.a1 Prupe.1G550800_v2.0.a1 Prupe.1G557100_v2.0.a1 Prupe.1G557200_v2.0.a1 Prupe.1G557200_v2.0.a1 Prupe.1G557300_v2.0.a1
pyrus_communis pycom02g02080 pycom02g02090 pycom02g02110 pycom02g03220 pycom02g03260 pycom02g03370 pycom02g03390 pycom02g03410 pycom02g03420 pycom02g03450 pycom02g03500 pycom02g03730 pycom02g03770 pycom02g04400 pycom02g04410 pycom02g04430 pycom02g04450 pycom02g04460 pycom02g04490 pycom02g05200 pycom02g25710 pycom02g25750 pycom02g25790 pycom02g25810 pycom02g25830 pycom04g00920 pycom04g00930 pycom04g00940 pycom05g29300 pycom05g29310 pycom05g29380 pycom05g29410 pycom07g06310 pycom07g21420 pycom08g15070 pycom08g17540 pycom08g17560 pycom08g17570 pycom10g02670 pycom10g02680 pycom10g02700 pycom10g02770 pycom10g02790 pycom10g02800 pycom10g02810 pycom10g25020 pycom10g25030 pycom10g25050 pycom10g25080 pycom111g01990 pycom111g02000 pycom111g02030 pycom111g02040 pycom111g02050 pycom111g02060 pycom12g22710 pycom12g22720 pycom12g22730 pycom12g22740 pycom12g22750 pycom12g22770 pycom12g22780 pycom12g22790 pycom12g22800 pycom12g22810 pycom12g22830 pycom12g22840 pycom12g22860 pycom15g09440 pycom15g09480 pycom15g16120 pycom15g32380 pycom16g05970 pycom17g02100
rosa_chinensis RchiOBHm_Chr2g0144661 RchiOBHm_Chr4g0407491 RchiOBHm_Chr4g0407501 RchiOBHm_Chr4g0412381 RchiOBHm_Chr7g0208191 RchiOBHm_Chr7g0208201 RchiOBHm_Chr7g0208211 RchiOBHm_Chr7g0208221 RchiOBHm_Chr7g0228071 RchiOBHm_Chr7g0228121 RchiOBHm_Chr7g0230971 RchiOBHm_Chr7g0230981 RchiOBHm_Chr7g0233901 RchiOBHm_Chr7g0233951 RchiOBHm_Chr7g0233961 RchiOBHm_Chr7g0233991 RchiOBHm_Chr7g0234021 RchiOBHm_Chr7g0234151 RchiOBHm_Chr7g0234161 RchiOBHm_Chr7g0234241 RchiOBHm_Chr7g0234501 RchiOBHm_Chr7g0234641 RchiOBHm_Chr7g0234771 RchiOBHm_Chr7g0234781 RchiOBHm_Chr7g0234991 RchiOBHm_Chr7g0241291 RchiOBHm_Chr7g0241301
rosa_laevigata RLG00000001186 RLG00000001187 RLG00000001212 RLG00000001226 RLG00000001233 RLG00000001590 RLG00000003242 RLG00000003243 RLG00000003249 RLG00000008688 RLG00000008690
rosa_multiflora Rmu_co7966574.1_g000001 Rmu_co8120830.1_g000001 Rmu_co8142226.1_g000001 Rmu_co8293083.1_g000001 Rmu_co8351227.1_g000001 Rmu_co8380159.1_g000001 Rmu_co8456505.1_g000001 Rmu_sc0000676.1_g000025 Rmu_sc0000706.1_g000018 Rmu_sc0000706.1_g000040 Rmu_sc0000740.1_g000029 Rmu_sc0000740.1_g000032 Rmu_sc0000740.1_g000037 Rmu_sc0000740.1_g000040 Rmu_sc0000762.1_g000003 Rmu_sc0000762.1_g000019 Rmu_sc0000762.1_g000034 Rmu_sc0000762.1_g000069 Rmu_sc0000892.1_g000001 Rmu_sc0000945.1_g000040 Rmu_sc0001913.1_g000037 Rmu_sc0001913.1_g000041 Rmu_sc0001913.1_g000049 Rmu_sc0002775.1_g000017 Rmu_sc0002775.1_g000018 Rmu_sc0002775.1_g000020 Rmu_sc0002775.1_g000021 Rmu_sc0002775.1_g000022 Rmu_sc0002775.1_g000025 Rmu_sc0002775.1_g000026 Rmu_sc0002775.1_g000028 Rmu_sc0002775.1_g000030 Rmu_sc0004858.1_g000002 Rmu_sc0004864.1_g000022 Rmu_sc0005038.1_g000023 Rmu_sc0005840.1_g000014 Rmu_sc0006724.1_g000003 Rmu_sc0006824.1_g000005 Rmu_sc0007869.1_g000003 Rmu_sc0007869.1_g000005 Rmu_sc0007869.1_g000008 Rmu_sc0008646.1_g000007 Rmu_sc0009637.1_g000009 Rmu_sc0010500.1_g000002 Rmu_sc0010500.1_g000012 Rmu_sc0012403.1_g000025 Rmu_sc0013768.1_g000025 Rmu_sc0015625.1_g000001 Rmu_sc0019715.1_g000001 Rmu_sc0021133.1_g000002 Rmu_sc0021904.1_g000001 Rmu_sc0021904.1_g000003 Rmu_sc0021904.1_g000004 Rmu_sc0022054.1_g000001 Rmu_sc0026025.1_g000001 Rmu_sc0036828.1_g000002 Rmu_sc0041808.1_g000001 Rmu_ssc0000012.1_g000001
rosa_roxburghii Rroxscaffold_3G00226370 Rroxscaffold_3G00226860 Rroxscaffold_3G00226900 Rroxscaffold_3G00226930 Rroxscaffold_3G00226940 Rroxscaffold_3G00226970 Rroxscaffold_3G00226980 Rroxscaffold_3G00231530 Rroxscaffold_3G00237090 Rroxscaffold_3G00250760 Rroxscaffold_3G00250800
rosa_rugosa Rorug04G0082300 Rorug07G0101800 Rorug07G0102000 Rorug07G0102100 Rorug07G0221200 Rorug07G0245700 Rorug07G0245700 Rorug07G0245700 Rorug07G0263900 Rorug07G0264000 Rorug07G0264000 Rorug07G0264500 Rorug07G0282900 Rorug07G0282900 Rorug07G0283100 Rorug07G0283200.1 Rorug07G0283300 Rorug07G0283900 Rorug07G0284000 Rorug07G0284100 Rorug07G0284300 Rorug07G0284400 Rorug07G0284500 Rorug07G0285000 Rorug07G0285200 Rorug07G0285300 Rorug07G0285500 Rorug07G0286800 Rorug07G0286900 Rorug07G0287100 Rorug07G0287200
rosa_samantha Rh7AG236100 Rh7AG236200 Rh7AG236300 Rh7AG440000 Rh7AG440100 Rh7BG411900 Rh7BG412000 Rh7BG412600 Rh7CG251300 Rh7CG417700 Rh7CG460100 Rh7DG242100 Rh7DG242200 Rh7DG242300 Rh7DG429300
rosa_wichuraiana Rw0G005070 Rw0G010780 Rw0G012530 Rw7G020110 Rw7G020160 Rw7G020170 Rw7G033210 Rw7G033230 Rw7G033250 Rw7G034540 Rw7G035150 Rw7G036190 Rw7G036210 Rw7G036260 Rw7G036270 Rw7G036290 Rw7G036300 Rw7G036310 Rw7G036350 Rw7G036360 Rw7G036440 Rw7G036510 Rw7G036530 Rw7G036570 Rw7G036590 Rw7G036730

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1019, 2258
AarI CACCTGC 1 cut(s) 1424
Acc36I ACCTGC 2 cut(s) 1424, 1911
AccB7I CCANNNNNTGG 1 cut(s) 304
AccBSI CCGCTC 1 cut(s) 488
AccI GTMKAC 1 cut(s) 1855
AccII CGCG 2 cut(s) 156, 278
AccIII TCCGGA 1 cut(s) 2143
AciI CCGC 7 cut(s) 226, 278, 488, 491, 1199, 1906, 1945
AclWI GGATC 5 cut(s) 254, 365, 1794, 1807, 2032
AcoI YGGCCR 1 cut(s) 53
AcuI CTGAAG 4 cut(s) 1096, 1286, 1650, 1659
AfaI GTAC 8 cut(s) 80, 438, 927, 1687, 1740, 1828, 1910, 2283
AfiI CCNNNNNNNGG 4 cut(s) 142, 304, 2146, 2493
AflIII ACRYGT 2 cut(s) 84, 379
AgeI ACCGGT 1 cut(s) 395
AgsI TTSAA 6 cut(s) 218, 589, 967, 1130, 1211, 2245
AjiI CACGTC 1 cut(s) 85
AjnI CCWGG 5 cut(s) 297, 1887, 2310, 2382, 2486
Alw21I GWGCWC 1 cut(s) 1110
Alw26I GTCTC 6 cut(s) 299, 415, 1512, 1628, 2384, 2408
AlwI GGATC 5 cut(s) 254, 365, 1794, 1807, 2032
AlwNI CAGNNNCTG 1 cut(s) 304
Aor13HI TCCGGA 1 cut(s) 2143
AoxI GGCC 9 cut(s) 38, 53, 2332, 2461, 2484, 2489, 2496, 2513, 2537
ApeKI GCWGC 5 cut(s) 473, 1430, 1464, 1671, 2121
ArsI GACNNNNNNTTYG 2 cut(s) 1383, 1415
AsiGI ACCGGT 1 cut(s) 395
Asp700I GAANNNNTTC 4 cut(s) 616, 831, 1281, 1536
AspA2I CCTAGG 2 cut(s) 2471, 2492
AspLEI GCGC 2 cut(s) 158, 1989
AsuC2I CCSGG 3 cut(s) 160, 1838, 2360
AsuHPI GGTGA 4 cut(s) 116, 650, 884, 1992
AsuII TTCGAA 1 cut(s) 2198
AvaII GGWCC 5 cut(s) 130, 1900, 2076, 2375, 2399
AvrII CCTAGG 2 cut(s) 2471, 2492
AxyI CCTNAGG 2 cut(s) 1143, 2500
BalI TGGCCA 1 cut(s) 55
BamHI GGATCC 1 cut(s) 1799
BanII GRGCYC 1 cut(s) 1110
BarI GAAGNNNNNNTAC 4 cut(s) 420, 452, 1162, 1194
BauI CACGAG 1 cut(s) 621
BbsI GAAGAC 3 cut(s) 114, 253, 1155
Bbv12I GWGCWC 1 cut(s) 1110
BbvI GCAGC 5 cut(s) 460, 1417, 1451, 1683, 2108
BccI CCATC 7 cut(s) 40, 52, 282, 609, 737, 1776, 2293
BceAI ACGGC 1 cut(s) 969
BciT130I CCWGG 5 cut(s) 299, 1889, 2312, 2384, 2488
BciVI GTATCC 1 cut(s) 1870
BclI TGATCA 1 cut(s) 897
BcnI CCSGG 3 cut(s) 160, 1838, 2360
BcoDI GTCTC 6 cut(s) 299, 415, 1512, 1628, 2384, 2408
BfaI CTAG 7 cut(s) 462, 773, 2379, 2403, 2451, 2472, 2493
BfmI CTRYAG 3 cut(s) 1431, 1852, 2027
BfoI RGCGCY 1 cut(s) 1990
BfuAI ACCTGC 2 cut(s) 1424, 1911
BfuI GTATCC 1 cut(s) 1870
BglII AGATCT 1 cut(s) 1664
BisI GCNGC 6 cut(s) 474, 489, 1431, 1465, 1672, 2122
BlnI CCTAGG 2 cut(s) 2471, 2492
BlsI GCNGC 6 cut(s) 475, 490, 1432, 1466, 1673, 2123
Bme1390I CCNGG 8 cut(s) 160, 299, 1838, 1889, 2312, 2360, 2384, 2488
Bme18I GGWCC 5 cut(s) 130, 1900, 2076, 2375, 2399
BmgBI CACGTC 1 cut(s) 85
BmiI GGNNCC 2 cut(s) 1801, 2514
BmrFI CCNGG 8 cut(s) 160, 299, 1838, 1889, 2312, 2360, 2384, 2488
BmsI GCATC 5 cut(s) 17, 169, 897, 1315, 1491
BpiI GAAGAC 3 cut(s) 114, 253, 1155
BplI GAGNNNNNCTC 2 cut(s) 460, 492
BpmI CTGGAG 2 cut(s) 281, 1770
Bpu10I CCTNAGC 1 cut(s) 2466
Bpu14I TTCGAA 1 cut(s) 2198
BpuEI CTTGAG 6 cut(s) 112, 787, 1124, 1150, 1352, 1715
BpuMI CCSGG 3 cut(s) 160, 1838, 2360
BsaAI YACGTR 2 cut(s) 78, 142
BsaJI CCNNGG 7 cut(s) 159, 183, 235, 2289, 2358, 2471, 2492
BsaWI WCCGGW 3 cut(s) 58, 395, 2143
Bsc4I CCNNNNNNNGG 4 cut(s) 142, 304, 2146, 2493
Bse118I RCCGGY 2 cut(s) 395, 2459
Bse1I ACTGG 2 cut(s) 605, 1416
Bse21I CCTNAGG 2 cut(s) 1143, 2500
Bse3DI GCAATG 1 cut(s) 2079
BseAI TCCGGA 1 cut(s) 2143
BseBI CCWGG 5 cut(s) 299, 1889, 2312, 2384, 2488
BseDI CCNNGG 7 cut(s) 159, 183, 235, 2289, 2358, 2471, 2492
BseGI GGATG 7 cut(s) 17, 44, 184, 601, 2285, 2368, 2416
BseLI CCNNNNNNNGG 4 cut(s) 142, 304, 2146, 2493
BseMI GCAATG 1 cut(s) 2079
BseMII CTCAG 5 cut(s) 279, 1324, 1987, 2327, 2514
BseNI ACTGG 2 cut(s) 605, 1416
BseRI GAGGAG 2 cut(s) 498, 1349
BseXI GCAGC 5 cut(s) 460, 1417, 1451, 1683, 2108
BsgI GTGCAG 2 cut(s) 58, 2107
Bsh1236I CGCG 2 cut(s) 156, 278
Bsh1285I CGRYCG 1 cut(s) 952
BshFI GGCC 9 cut(s) 40, 55, 2334, 2463, 2486, 2491, 2498, 2515, 2539
BshTI ACCGGT 1 cut(s) 395
BsiEI CGRYCG 1 cut(s) 952
BsiHKAI GWGCWC 1 cut(s) 1110
BsiSI CCGG 7 cut(s) 59, 159, 396, 1837, 2144, 2360, 2460
BsiWI CGTACG 1 cut(s) 78
BslI CCNNNNNNNGG 4 cut(s) 142, 304, 2146, 2493
BsmAI GTCTC 6 cut(s) 299, 415, 1512, 1628, 2384, 2408
BsmI GAATGC 1 cut(s) 973
BsnI GGCC 9 cut(s) 40, 55, 2334, 2463, 2486, 2491, 2498, 2515, 2539
Bsp119I TTCGAA 1 cut(s) 2198
Bsp1286I GDGCHC 1 cut(s) 1110
Bsp13I TCCGGA 1 cut(s) 2143
Bsp143I GATC 8 cut(s) 246, 370, 897, 949, 1030, 1664, 1799, 2037
BspACI CCGC 7 cut(s) 226, 278, 488, 491, 1199, 1906, 1945
BspANI GGCC 9 cut(s) 40, 55, 2334, 2463, 2486, 2491, 2498, 2515, 2539
BspCNI CTCAG 5 cut(s) 278, 1323, 1986, 2328, 2513
BspEI TCCGGA 1 cut(s) 2143
BspFNI CGCG 2 cut(s) 156, 278
BspHI TCATGA 1 cut(s) 748
BspLI GGNNCC 2 cut(s) 1801, 2514
BspMAI CTGCAG 1 cut(s) 1435
BspMI ACCTGC 2 cut(s) 1424, 1911
BspPI GGATC 5 cut(s) 254, 365, 1794, 1807, 2032
BspQI GCTCTTC 1 cut(s) 1115
BspT104I TTCGAA 1 cut(s) 2198
BsrBI CCGCTC 1 cut(s) 488
BsrDI GCAATG 1 cut(s) 2079
BsrFI RCCGGY 2 cut(s) 395, 2459
BsrI ACTGG 2 cut(s) 605, 1416
BssAI RCCGGY 2 cut(s) 395, 2459
BssECI CCNNGG 7 cut(s) 159, 183, 235, 2289, 2358, 2471, 2492
BssMI GATC 8 cut(s) 246, 370, 897, 949, 1030, 1664, 1799, 2037
BssSI CACGAG 1 cut(s) 621
BssT1I CCWWGG 3 cut(s) 235, 2471, 2492
Bst2BI CACGAG 1 cut(s) 621
Bst2UI CCWGG 5 cut(s) 299, 1889, 2312, 2384, 2488
Bst4CI ACNGT 7 cut(s) 436, 718, 761, 1187, 1477, 2031, 2051
Bst6I CTCTTC 3 cut(s) 1115, 1165, 1626
BstAPI GCANNNNNTGC 1 cut(s) 1510
BstBAI YACGTR 2 cut(s) 78, 142
BstBI TTCGAA 1 cut(s) 2198
BstC8I GCNNGC 7 cut(s) 306, 502, 1122, 1203, 1793, 2219, 2461
BstENI CCTNNNNNAGG 1 cut(s) 2144
BstF5I GGATG 7 cut(s) 17, 44, 184, 601, 2285, 2368, 2416
BstFNI CGCG 2 cut(s) 156, 278
BstH2I RGCGCY 1 cut(s) 1990
BstHHI GCGC 2 cut(s) 158, 1989
BstKTI GATC 8 cut(s) 249, 373, 900, 952, 1033, 1667, 1802, 2040
BstMAI GTCTC 6 cut(s) 299, 415, 1512, 1628, 2384, 2408
BstMBI GATC 8 cut(s) 246, 370, 897, 949, 1030, 1664, 1799, 2037
BstMCI CGRYCG 1 cut(s) 952
BstMWI GCNNNNNNNGC 5 cut(s) 497, 1510, 1792, 1942, 2460
BstNI CCWGG 5 cut(s) 299, 1889, 2312, 2384, 2488
BstNSI RCATGY 2 cut(s) 383, 1124
BstSCI CCNGG 8 cut(s) 158, 297, 1836, 1887, 2310, 2358, 2382, 2486
BstSFI CTRYAG 3 cut(s) 1431, 1852, 2027
BstUI CGCG 2 cut(s) 156, 278
BstV1I GCAGC 5 cut(s) 460, 1417, 1451, 1683, 2108
BstV2I GAAGAC 3 cut(s) 114, 253, 1155
BstX2I RGATCY 3 cut(s) 246, 1664, 1799
BstXI CCANNNNNNTGG 3 cut(s) 51, 608, 1667
BstYI RGATCY 3 cut(s) 246, 1664, 1799
Bsu36I CCTNAGG 2 cut(s) 1143, 2500
BsuI GTATCC 1 cut(s) 1870
BsuRI GGCC 9 cut(s) 40, 55, 2334, 2463, 2486, 2491, 2498, 2515, 2539
BtgZI GCGATG 1 cut(s) 9
BtrI CACGTC 1 cut(s) 85
BtsCI GGATG 7 cut(s) 17, 44, 184, 601, 2285, 2368, 2416
BtsI GCAGTG 2 cut(s) 818, 2461
BtsIMutI CAGTG 3 cut(s) 818, 1262, 2461
BveI ACCTGC 2 cut(s) 1424, 1911
Cac8I GCNNGC 7 cut(s) 306, 502, 1122, 1203, 1793, 2219, 2461
CaiI CAGNNNCTG 1 cut(s) 304
CciI TCATGA 1 cut(s) 748
CfoI GCGC 2 cut(s) 158, 1989
Cfr10I RCCGGY 2 cut(s) 395, 2459
CsiI ACCWGGT 1 cut(s) 1887
Csp6I GTAC 8 cut(s) 79, 437, 926, 1686, 1739, 1827, 1909, 2282
CspAI ACCGGT 1 cut(s) 395
CspCI CAANNNNNGTGG 4 cut(s) 1155, 1190, 1349, 1384
CviQI GTAC 8 cut(s) 79, 437, 926, 1686, 1739, 1827, 1909, 2282
DpnI GATC 8 cut(s) 248, 372, 899, 951, 1032, 1666, 1801, 2039
DpnII GATC 8 cut(s) 246, 370, 897, 949, 1030, 1664, 1799, 2037
EaeI YGGCCR 1 cut(s) 53
Eam1104I CTCTTC 3 cut(s) 1115, 1165, 1626
EarI CTCTTC 3 cut(s) 1115, 1165, 1626
EciI GGCGGA 1 cut(s) 506
Ecl136II GAGCTC 1 cut(s) 1108
Eco130I CCWWGG 3 cut(s) 235, 2471, 2492
Eco147I AGGCCT 2 cut(s) 2334, 2491
Eco24I GRGCYC 1 cut(s) 1110
Eco32I GATATC 1 cut(s) 2370
Eco47I GGWCC 5 cut(s) 130, 1900, 2076, 2375, 2399
Eco53kI GAGCTC 1 cut(s) 1108
Eco57I CTGAAG 4 cut(s) 1096, 1286, 1650, 1659
Eco81I CCTNAGG 2 cut(s) 1143, 2500
EcoICRI GAGCTC 1 cut(s) 1108
EcoNI CCTNNNNNAGG 1 cut(s) 2144
EcoO109I RGGNCCY 5 cut(s) 2375, 2399, 2496, 2513, 2538
EcoRII CCWGG 5 cut(s) 297, 1887, 2310, 2382, 2486
EcoRV GATATC 1 cut(s) 2370
EcoT14I CCWWGG 3 cut(s) 235, 2471, 2492
EcoT22I ATGCAT 2 cut(s) 973, 1506
EcoT38I GRGCYC 1 cut(s) 1110
ErhI CCWWGG 3 cut(s) 235, 2471, 2492
FalI AAGNNNNNCTT 4 cut(s) 816, 848, 894, 926
FauNDI CATATG 1 cut(s) 1364
FbaI TGATCA 1 cut(s) 897
FblI GTMKAC 1 cut(s) 1855
Fnu4HI GCNGC 6 cut(s) 474, 489, 1431, 1465, 1672, 2122
FokI GGATG 7 cut(s) 4, 31, 191, 588, 2272, 2375, 2423
FriOI GRGCYC 1 cut(s) 1110
Fsp4HI GCNGC 6 cut(s) 474, 489, 1431, 1465, 1672, 2122
FspBI CTAG 7 cut(s) 462, 773, 2379, 2403, 2451, 2472, 2493
GlaI GCGC 2 cut(s) 157, 1988
GluI GCNGC 6 cut(s) 474, 489, 1431, 1465, 1672, 2122
GsuI CTGGAG 2 cut(s) 281, 1770
HaeII RGCGCY 1 cut(s) 1990
HaeIII GGCC 9 cut(s) 40, 55, 2334, 2463, 2486, 2491, 2498, 2515, 2539
HapII CCGG 7 cut(s) 59, 159, 396, 1837, 2144, 2360, 2460
HhaI GCGC 2 cut(s) 158, 1989
Hin6I GCGC 2 cut(s) 156, 1987
HinP1I GCGC 2 cut(s) 156, 1987
HincII GTYRAC 3 cut(s) 553, 940, 1759
HindII GTYRAC 3 cut(s) 553, 940, 1759
HindIII AAGCTT 2 cut(s) 1318, 1763
HinfI GANTC 9 cut(s) 281, 617, 702, 800, 989, 1547, 1644, 2068, 2176
HpaII CCGG 7 cut(s) 59, 159, 396, 1837, 2144, 2360, 2460
HphI GGTGA 4 cut(s) 116, 650, 884, 1992
Hpy166II GTNNAC 8 cut(s) 553, 673, 940, 1196, 1759, 1856, 1900, 2342
Hpy188I TCNGA 9 cut(s) 101, 167, 268, 386, 841, 1074, 1313, 1459, 1922
Hpy8I GTNNAC 8 cut(s) 553, 673, 940, 1196, 1759, 1856, 1900, 2342
Hpy99I CGWCG 2 cut(s) 86, 1926
HpyAV CCTTC 6 cut(s) 133, 384, 1230, 1409, 1634, 2150
HpyCH4III ACNGT 7 cut(s) 436, 718, 761, 1187, 1477, 2031, 2051
HpyCH4IV ACGT 7 cut(s) 77, 84, 141, 649, 1570, 1911, 2159
HpyF10VI GCNNNNNNNGC 5 cut(s) 497, 1510, 1792, 1942, 2460
HpySE526I ACGT 7 cut(s) 77, 84, 141, 649, 1570, 1911, 2159
HspAI GCGC 2 cut(s) 156, 1987
Kpn2I TCCGGA 1 cut(s) 2143
KroI GCCGGC 1 cut(s) 2459
KroNI GCCGGC 1 cut(s) 2461
Ksp22I TGATCA 1 cut(s) 897
Kzo9I GATC 8 cut(s) 246, 370, 897, 949, 1030, 1664, 1799, 2037
LguI GCTCTTC 1 cut(s) 1115
LmnI GCTCC 6 cut(s) 301, 485, 1239, 1728, 1751, 1933
Lsp1109I GCAGC 5 cut(s) 460, 1417, 1451, 1683, 2108
LweI GCATC 5 cut(s) 17, 169, 897, 1315, 1491
MabI ACCWGGT 1 cut(s) 1887
MaeI CTAG 7 cut(s) 462, 773, 2379, 2403, 2451, 2472, 2493
MaeII ACGT 7 cut(s) 77, 84, 141, 649, 1570, 1911, 2159
MaeIII GTNAC 3 cut(s) 1041, 1177, 1471
MalI GATC 8 cut(s) 248, 372, 899, 951, 1032, 1666, 1801, 2039
MbiI CCGCTC 1 cut(s) 488
MboI GATC 8 cut(s) 246, 370, 897, 949, 1030, 1664, 1799, 2037
MfeI CAATTG 3 cut(s) 218, 962, 1881
MflI RGATCY 3 cut(s) 246, 1664, 1799
MhlI GDGCHC 1 cut(s) 1110
MlsI TGGCCA 1 cut(s) 55
MluNI TGGCCA 1 cut(s) 55
MlyI GAGTC 3 cut(s) 794, 1638, 2170
MmeI TCCRAC 4 cut(s) 589, 661, 1750, 1945
Mox20I TGGCCA 1 cut(s) 55
Mph1103I ATGCAT 2 cut(s) 973, 1506
MroI TCCGGA 1 cut(s) 2143
MroNI GCCGGC 1 cut(s) 2459
MroXI GAANNNNTTC 4 cut(s) 616, 831, 1281, 1536
MscI TGGCCA 1 cut(s) 55
MseI TTAA 9 cut(s) 149, 627, 848, 1479, 1778, 1968, 2043, 2115, 2548
MslI CAYNNNNRTG 3 cut(s) 49, 893, 1382
Msp20I TGGCCA 1 cut(s) 55
MspA1I CMGCKG 2 cut(s) 571, 1201
MspI CCGG 7 cut(s) 59, 159, 396, 1837, 2144, 2360, 2460
MspR9I CCNGG 8 cut(s) 160, 299, 1838, 1889, 2312, 2360, 2384, 2488
MunI CAATTG 3 cut(s) 218, 962, 1881
Mva1269I GAATGC 1 cut(s) 973
MvaI CCWGG 5 cut(s) 299, 1889, 2312, 2384, 2488
MvnI CGCG 2 cut(s) 156, 278
MwoI GCNNNNNNNGC 5 cut(s) 497, 1510, 1792, 1942, 2460
NaeI GCCGGC 1 cut(s) 2461
NciI CCSGG 3 cut(s) 160, 1838, 2360
NdeI CATATG 1 cut(s) 1364
NdeII GATC 8 cut(s) 246, 370, 897, 949, 1030, 1664, 1799, 2037
NgoMIV GCCGGC 1 cut(s) 2459
NlaIV GGNNCC 2 cut(s) 1801, 2514
NmeAIII GCCGAG 1 cut(s) 208
NsiI ATGCAT 2 cut(s) 973, 1506
NspI RCATGY 2 cut(s) 383, 1124
NspV TTCGAA 1 cut(s) 2198
PaeI GCATGC 1 cut(s) 1124
PagI TCATGA 1 cut(s) 748
PaqCI CACCTGC 1 cut(s) 1424
PceI AGGCCT 2 cut(s) 2334, 2491
PciI ACATGT 1 cut(s) 379
PciSI GCTCTTC 1 cut(s) 1115
PctI GAATGC 1 cut(s) 973
PdiI GCCGGC 1 cut(s) 2461
PdmI GAANNNNTTC 4 cut(s) 616, 831, 1281, 1536
PfeI GAWTC 6 cut(s) 281, 617, 702, 989, 1547, 2068
Pfl23II CGTACG 1 cut(s) 78
PflFI GACNNNGTC 1 cut(s) 944
PflMI CCANNNNNTGG 1 cut(s) 304
PfoI TCCNGGA 1 cut(s) 297
PinAI ACCGGT 1 cut(s) 395
PkrI GCNGC 6 cut(s) 475, 490, 1432, 1466, 1673, 2123
Ple19I CGATCG 1 cut(s) 952
PleI GAGTC 3 cut(s) 794, 1638, 2170
PpsI GAGTC 3 cut(s) 794, 1638, 2170
Ppu21I YACGTR 2 cut(s) 78, 142
PpuMI RGGWCCY 2 cut(s) 2375, 2399
PscI ACATGT 1 cut(s) 379
PsiI TTATAA 2 cut(s) 1019, 2258
Psp124BI GAGCTC 1 cut(s) 1110
Psp5II RGGWCCY 2 cut(s) 2375, 2399
Psp6I CCWGG 5 cut(s) 297, 1887, 2310, 2382, 2486
PspGI CCWGG 5 cut(s) 297, 1887, 2310, 2382, 2486
PspLI CGTACG 1 cut(s) 78
PspN4I GGNNCC 2 cut(s) 1801, 2514
PspPPI RGGWCCY 2 cut(s) 2375, 2399
PsrI GAACNNNNNNTAC 2 cut(s) 71, 103
PstI CTGCAG 1 cut(s) 1435
PstNI CAGNNNCTG 1 cut(s) 304
PsuI RGATCY 3 cut(s) 246, 1664, 1799
PsyI GACNNNGTC 1 cut(s) 944
PvuI CGATCG 1 cut(s) 952
PvuII CAGCTG 1 cut(s) 571
RsaI GTAC 8 cut(s) 80, 438, 927, 1687, 1740, 1828, 1910, 2283
RsaNI GTAC 8 cut(s) 79, 437, 926, 1686, 1739, 1827, 1909, 2282
RseI CAYNNNNRTG 3 cut(s) 49, 893, 1382
SacI GAGCTC 1 cut(s) 1110
SapI GCTCTTC 1 cut(s) 1115
SaqAI TTAA 9 cut(s) 149, 627, 848, 1479, 1778, 1968, 2043, 2115, 2548
SatI GCNGC 6 cut(s) 474, 489, 1431, 1465, 1672, 2122
Sau3AI GATC 8 cut(s) 246, 370, 897, 949, 1030, 1664, 1799, 2037
SchI GAGTC 3 cut(s) 794, 1638, 2170
ScrFI CCNGG 8 cut(s) 160, 299, 1838, 1889, 2312, 2360, 2384, 2488
SduI GDGCHC 1 cut(s) 1110
SexAI ACCWGGT 1 cut(s) 1887
SfaNI GCATC 5 cut(s) 17, 169, 897, 1315, 1491
SfcI CTRYAG 3 cut(s) 1431, 1852, 2027
SfuI TTCGAA 1 cut(s) 2198
SinI GGWCC 5 cut(s) 130, 1900, 2076, 2375, 2399
SmiMI CAYNNNNRTG 3 cut(s) 49, 893, 1382
SmlI CTYRAG 6 cut(s) 91, 802, 1103, 1165, 1331, 1730
SmoI CTYRAG 6 cut(s) 91, 802, 1103, 1165, 1331, 1730
SphI GCATGC 1 cut(s) 1124
SseBI AGGCCT 2 cut(s) 2334, 2491
SsiI CCGC 7 cut(s) 226, 278, 488, 491, 1199, 1906, 1945
SspI AATATT 1 cut(s) 644
SspMI CTAG 7 cut(s) 462, 773, 2379, 2403, 2451, 2472, 2493
SstI GAGCTC 1 cut(s) 1110
StuI AGGCCT 2 cut(s) 2334, 2491
StyD4I CCNGG 8 cut(s) 158, 297, 1836, 1887, 2310, 2358, 2382, 2486
StyI CCWWGG 3 cut(s) 235, 2471, 2492
TaaI ACNGT 7 cut(s) 436, 718, 761, 1187, 1477, 2031, 2051
TaiI ACGT 7 cut(s) 80, 87, 144, 652, 1573, 1914, 2162
TatI WGTACW 3 cut(s) 436, 1685, 1738
TauI GCSGC 1 cut(s) 491
TfiI GAWTC 6 cut(s) 281, 617, 702, 989, 1547, 2068
Tru1I TTAA 9 cut(s) 149, 627, 848, 1479, 1778, 1968, 2043, 2115, 2548
Tru9I TTAA 9 cut(s) 149, 627, 848, 1479, 1778, 1968, 2043, 2115, 2548
TscAI CASTG 3 cut(s) 825, 1269, 2461
TseI GCWGC 5 cut(s) 473, 1430, 1464, 1671, 2121
TspGWI ACGGA 1 cut(s) 143
TspRI CASTG 3 cut(s) 825, 1269, 2461
Tth111I GACNNNGTC 1 cut(s) 944
Van91I CCANNNNNTGG 1 cut(s) 304
VpaK11BI GGWCC 5 cut(s) 130, 1900, 2076, 2375, 2399
XagI CCTNNNNNAGG 1 cut(s) 2144
XceI RCATGY 2 cut(s) 383, 1124
XcmI CCANNNNNNNNNTGG 1 cut(s) 48
XmaJI CCTAGG 2 cut(s) 2471, 2492
XmiI GTMKAC 1 cut(s) 1855
XmnI GAANNNNTTC 4 cut(s) 616, 831, 1281, 1536
XspI CTAG 7 cut(s) 462, 773, 2379, 2403, 2451, 2472, 2493
Zsp2I ATGCAT 2 cut(s) 973, 1506
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.