MD02G1306700.v1.1

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0000055
Physical Location & Seq
Reverse (-)
35993934 .. 35999234
5301 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1306700.v1.1.491

Sequence Viewer

Length: 2838 bp
ATGCCAAATTGCCAACTGATGAAGATGGCCTCCTCCTCCTCTTCGTCAAAATGTAGCAAGTACGACGTGTTCTTGAGCTTCAGAGGCGAAACGCGCAAGAGCTTGACGGACAACCTCTACTGCACATTGAAAGACCACAGATTCAACGTCTTTATCGACGAGAACGAATTGACCAGAGGCGAGATTATCACACCAGCACTGAAGCAAGCAGTCAAAGACTCTAGGCTTGCTGCTATCATCTTCTCAAGCGGGTATGCCGAGTCGAGATGGTGTCTTGATGAGCTGGTCGAGATCTTGGCCAGCAGAAGAACAATGGGGCAAATGGTTTTGCCAATTTTCTACAATATCGATCCTTCCGATGTCAGGCATCAGAGGGGTGCTTTTGCGGAAGCATTTCGGAGACATCAAGAGCGCTTCCCTAAAATGGTGCAGACCTGGAAAGATGCTCTTAATGAAGCTGCAGAGTTGGCCGGCGAGGATTTTAGTGTGACTGACGGGTATAAAGGAAGGTTTGTCAGGAAAATTGTTGACGAGATCACTAGGAAACTGAAGAACACAAACTTAGCCGTAGCCCCAAACCCAGTTGGAATAGATTCTCGCGTGCAAGAAATTGGTAACCATTTGGATGTCGAAGGATCATATGATGTTCGCATAATCGGAATTTGGGGTATGGGCGGACTGGGTAAAACAACGGTTGCCAAAGCCATTTTCAACAAATATCAGGGTATGTTTCAATGTGCAAGTTTCATTCCTAATGTGAGAGAAGATAAGAAACTGGTTGATTCGCAAAACACACTTCTTTCTGATGTATTGAGATCGGGAAACATAAAGGTTAGTAGAGCTGATGAAGGGACCGAGGATATAAAAAGAAGACTTGGCAACCTAAAGGTACTTGTCATAATTGATGATGTAGACAGCGTGGACCAGTTAGATGCATTAGGTATAGAACGTGACTCGTTTGGTCCAAGAAGTAGAATTGTCATAACAACAAGAAATGAACATTTGCTAGAGGCACTAAAACCGGATAAGATATGTCATCTACCAGCAATGAATAAAGAAGAATCTCTTGAGCTCCTTAGTTGGCGTGCCTTTAGTAAGAATTATCCTAATGAAGAATATGTTCAAGTCTCAAGAGAAGTTGTTGAATATTGTGGTGGTTTGCCACTGGCACTTCAAGATTTAGGGACTTATCTTCGTGCAAAAAGCACAAGTGAATGGAAAAGTGCATTGGGTAAATGGAAAAGATCGCGGCCTTATCCGGCAGTTCACAAAAGGCTTAAGATAAGCTATGATGGGCTAACCGATGATGATGTGAAGTCTATGTTCCTTGATATATCCTGTTTCTTTATTGGAATGAACAAGGACTATGTCATGAAAATACTGGATCAACATGACTGTAATACAATTAGGGAACTGCATGACCAATGCCTTGTAACTGTTGATACAGAAGGCAATCTGATGATGCATGGTTTGATTCGTGACACTGGCAGAGAAATTGTACGTGAAAAATCCCCGGACATCCTTGGAAAGCGCTGTAGATTGTGGGATCACGAAGATGTAAAACGTGTATTGAGGACCAAAACTGGAAGGGAAGAAGTTCAAGGACTCTCTTTAGATTTGTCAGAACGTCACAAGCCTAGCTTCAGTACTGAAGCATTTAGAGAGATGCACGGATTGAGATTACTCAAACTCAAAGGCGTACAGTTCACTGGACACTGCGAAGACCTTTCCGAACACCTTTCCAAAGAGTTAAGATGGTTGTGTTGGCTTGAGTTTCCTATGAAGGGCATACCCAGAGATTTTAATCAAACAAACCTAGTTGATATCGACCTGAGTCATAGCAACATACAAGTTTGGGAGGACTCCAACGTGACACTTGAGAAGTTGAAGTTTCTTAATCTTGGATACTGCAATTGCCTGATCAGATTACCCGACTTCTCAAAAATCCCAAATCTTGAGAGATTGATACTCAAAGATTGCAAAAACCTTCAGGCAATACCAGCTTTACCAAAAAATTTGGAAATCCTGGAAGCGGATGATTGCATTGCATTGGAGAAAATGCCCAACTTTTCAGAGATGTCAAGGATGAGAGAATTGCATCTGAATCACTCCCCCAAACTCTCTGACATTCTAGGCTTGGATAAGGCATTAAACTCCATGACAAGGATTCATATGGAAGGGTGCACCAATCTCACTGCTGCTTTTAAGGAAGCAATCCTACAGGGATGGAGTGCGAGCGGAAATGGTGGCCTTTTTCTCCCTGGAAATGAAATTCCGTCATGGGTTACGCCTATCGACCCTCAGGGTAAAATAGTTGTGCCACAAAGTTTTGGTGATTTAAAAGCATTGACTGTGTGCATTATTTATTCTTCAGACGACTCCCAATCTGGTGGCTCTCTTTGCATTCATGTTGCAAATTGTACCCAGAACACCGAATTTCTCATCTCTCCTATGCGTGCCACTGTAATAACTTCCCATGAAAATTATCTTTGGCTGGGACATTTGTCAAACTATAAGCTTAATGTGAAAGGCGGAGACAAGATAAATGTTAGTGCACGCTTTGTAGGACCAGTGGCTACTGATGATATTCATCTCAGGGTGAAGAAGATAGGGATTAATCTGGAAAAGGAAAAATTAATCAATGAATATGCGCTCGAATGTCAATCTATGCCATATTCAAGTGAATGCAATGAGGACGGCAATCATAAGGCAGCATGGAGTGATGACTTGTCCCATGAGAATCAATCCCGCAAAAGACTGAGGTCTGATCCCAGTGTTGAAAGAAAAGCAGAAGAAGAAACTGATGAAATCATGCAAGAAGGAAGCAGCATGGGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

946

Amino Acids

107.09

Weight (kDa)

6.19

Isoelectric Point (pI)

45.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 20 - 187 3.9e-46 TIR domain
TIR_2 PF13676 23 - 118 1.2e-12 TIR domain
NB-ARC PF00931 199 - 367 2.3e-21 NB-ARC domain
WHD_ROQ1 PF23282 439 - 504 7.5e-12 Disease resistance protein Roq1-like, winged-helix domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000055)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G36930 AT5G36930 AT5G36930 AT5G36930
fragaria_vesca FvH4_3g45361 FvH4_3g45380 FvH4_5g21061 FvH4_5g21062 FvH4_5g21063 FvH4_5g32050 FvH4_5g32970 FvH4_5g32990 FvH4_5g34190 FvH4_5g34210 FvH4_5g34210 FvH4_5g34210 FvH4_5g34210 FvH4_5g34210 FvH4_5g34210 FvH4_5g34211 FvH4_5g34240 FvH4_5g34250
malus_domestica MD00G1011700.v1.1 MD00G1026100.v1.1 MD00G1026500.v1.1 MD00G1027000.v1.1 MD00G1027100.v1.1 MD00G1027700.v1.1 MD00G1027800.v1.1 MD00G1028000.v1.1 MD00G1220000.v1.1 MD02G1024400.v1.1 MD02G1024800.v1.1 MD02G1026000.v1.1 MD02G1026100.v1.1 MD02G1031800.v1.1 MD02G1038500.v1.1 MD02G1038700.v1.1 MD02G1039200.v1.1 MD02G1040600.v1.1 MD02G1040900.v1.1 MD02G1041200.v1.1 MD02G1041300.v1.1 MD02G1041700.v1.1 MD02G1042000.v1.1 MD02G1043800.v1.1 MD02G1043900.v1.1 MD02G1044300.v1.1 MD02G1045200.v1.1 MD02G1045300.v1.1 MD02G1051200.v1.1 MD02G1052200.v1.1 MD02G1052800.v1.1 MD02G1053100.v1.1 MD02G1053600.v1.1 MD02G1055500.v1.1 MD02G1063300.v1.1 MD02G1063400.v1.1 MD02G1063800.v1.1 MD02G1306500.v1.1 MD02G1306600.v1.1 MD02G1306700.v1.1 MD02G1306900.v1.1 MD02G1307200.v1.1 MD04G1011700.v1.1 MD05G1315400.v1.1 MD05G1316000.v1.1 MD05G1316700.v1.1 MD05G1317000.v1.1 MD05G1317300.v1.1 MD05G1317600.v1.1 MD05G1317700.v1.1 MD07G1102800.v1.1 MD07G1238200.v1.1 MD08G1175900.v1.1 MD08G1203600.v1.1 MD09G1024200.v1.1 MD09G1039600.v1.1 MD09G1044800.v1.1 MD09G1045100.v1.1 MD10G1038700.v1.1 MD10G1039000.v1.1 MD10G1039200.v1.1 MD10G1040000.v1.1 MD10G1040200.v1.1 MD10G1040600.v1.1 MD10G1040800.v1.1 MD10G1297300.v1.1 MD12G1247500.v1.1 MD12G1247900.v1.1 MD12G1248300.v1.1 MD12G1248800.v1.1 MD15G1103700.v1.1 MD15G1103800.v1.1 MD15G1104000.v1.1 MD15G1179600.v1.1 MD15G1182500.v1.1 MD16G1068200.v1.1 MD16G1068300.v1.1 MD17G1024600.v1.1 MD17G1024700.v1.1 MD17G1025000.v1.1 MD17G1025900.v1.1
prunus_persica Prupe.1G539800_v2.0.a1 Prupe.1G550800_v2.0.a1 Prupe.1G550800_v2.0.a1 Prupe.1G550800_v2.0.a1 Prupe.1G550800_v2.0.a1 Prupe.1G550800_v2.0.a1 Prupe.1G550800_v2.0.a1 Prupe.1G557100_v2.0.a1 Prupe.1G557200_v2.0.a1 Prupe.1G557200_v2.0.a1 Prupe.1G557300_v2.0.a1
pyrus_communis pycom02g02080 pycom02g02090 pycom02g02110 pycom02g03220 pycom02g03260 pycom02g03370 pycom02g03390 pycom02g03410 pycom02g03420 pycom02g03450 pycom02g03500 pycom02g03730 pycom02g03770 pycom02g04400 pycom02g04410 pycom02g04430 pycom02g04450 pycom02g04460 pycom02g04490 pycom02g05200 pycom02g25710 pycom02g25750 pycom02g25790 pycom02g25810 pycom02g25830 pycom04g00920 pycom04g00930 pycom04g00940 pycom05g29300 pycom05g29310 pycom05g29380 pycom05g29410 pycom07g06310 pycom07g21420 pycom08g15070 pycom08g17540 pycom08g17560 pycom08g17570 pycom10g02670 pycom10g02680 pycom10g02700 pycom10g02770 pycom10g02790 pycom10g02800 pycom10g02810 pycom10g25020 pycom10g25030 pycom10g25050 pycom10g25080 pycom111g01990 pycom111g02000 pycom111g02030 pycom111g02040 pycom111g02050 pycom111g02060 pycom12g22710 pycom12g22720 pycom12g22730 pycom12g22740 pycom12g22750 pycom12g22770 pycom12g22780 pycom12g22790 pycom12g22800 pycom12g22810 pycom12g22830 pycom12g22840 pycom12g22860 pycom15g09440 pycom15g09480 pycom15g16120 pycom15g32380 pycom16g05970 pycom17g02100
rosa_chinensis RchiOBHm_Chr2g0144661 RchiOBHm_Chr4g0407491 RchiOBHm_Chr4g0407501 RchiOBHm_Chr4g0412381 RchiOBHm_Chr7g0208191 RchiOBHm_Chr7g0208201 RchiOBHm_Chr7g0208211 RchiOBHm_Chr7g0208221 RchiOBHm_Chr7g0228071 RchiOBHm_Chr7g0228121 RchiOBHm_Chr7g0230971 RchiOBHm_Chr7g0230981 RchiOBHm_Chr7g0233901 RchiOBHm_Chr7g0233951 RchiOBHm_Chr7g0233961 RchiOBHm_Chr7g0233991 RchiOBHm_Chr7g0234021 RchiOBHm_Chr7g0234151 RchiOBHm_Chr7g0234161 RchiOBHm_Chr7g0234241 RchiOBHm_Chr7g0234501 RchiOBHm_Chr7g0234641 RchiOBHm_Chr7g0234771 RchiOBHm_Chr7g0234781 RchiOBHm_Chr7g0234991 RchiOBHm_Chr7g0241291 RchiOBHm_Chr7g0241301
rosa_laevigata RLG00000001186 RLG00000001187 RLG00000001212 RLG00000001226 RLG00000001233 RLG00000001590 RLG00000003242 RLG00000003243 RLG00000003249 RLG00000008688 RLG00000008690
rosa_multiflora Rmu_co7966574.1_g000001 Rmu_co8120830.1_g000001 Rmu_co8142226.1_g000001 Rmu_co8293083.1_g000001 Rmu_co8351227.1_g000001 Rmu_co8380159.1_g000001 Rmu_co8456505.1_g000001 Rmu_sc0000676.1_g000025 Rmu_sc0000706.1_g000018 Rmu_sc0000706.1_g000040 Rmu_sc0000740.1_g000029 Rmu_sc0000740.1_g000032 Rmu_sc0000740.1_g000037 Rmu_sc0000740.1_g000040 Rmu_sc0000762.1_g000003 Rmu_sc0000762.1_g000019 Rmu_sc0000762.1_g000034 Rmu_sc0000762.1_g000069 Rmu_sc0000892.1_g000001 Rmu_sc0000945.1_g000040 Rmu_sc0001913.1_g000037 Rmu_sc0001913.1_g000041 Rmu_sc0001913.1_g000049 Rmu_sc0002775.1_g000017 Rmu_sc0002775.1_g000018 Rmu_sc0002775.1_g000020 Rmu_sc0002775.1_g000021 Rmu_sc0002775.1_g000022 Rmu_sc0002775.1_g000025 Rmu_sc0002775.1_g000026 Rmu_sc0002775.1_g000028 Rmu_sc0002775.1_g000030 Rmu_sc0004858.1_g000002 Rmu_sc0004864.1_g000022 Rmu_sc0005038.1_g000023 Rmu_sc0005840.1_g000014 Rmu_sc0006724.1_g000003 Rmu_sc0006824.1_g000005 Rmu_sc0007869.1_g000003 Rmu_sc0007869.1_g000005 Rmu_sc0007869.1_g000008 Rmu_sc0008646.1_g000007 Rmu_sc0009637.1_g000009 Rmu_sc0010500.1_g000002 Rmu_sc0010500.1_g000012 Rmu_sc0012403.1_g000025 Rmu_sc0013768.1_g000025 Rmu_sc0015625.1_g000001 Rmu_sc0019715.1_g000001 Rmu_sc0021133.1_g000002 Rmu_sc0021904.1_g000001 Rmu_sc0021904.1_g000003 Rmu_sc0021904.1_g000004 Rmu_sc0022054.1_g000001 Rmu_sc0026025.1_g000001 Rmu_sc0036828.1_g000002 Rmu_sc0041808.1_g000001 Rmu_ssc0000012.1_g000001
rosa_roxburghii Rroxscaffold_3G00226370 Rroxscaffold_3G00226860 Rroxscaffold_3G00226900 Rroxscaffold_3G00226930 Rroxscaffold_3G00226940 Rroxscaffold_3G00226970 Rroxscaffold_3G00226980 Rroxscaffold_3G00231530 Rroxscaffold_3G00237090 Rroxscaffold_3G00250760 Rroxscaffold_3G00250800
rosa_rugosa Rorug04G0082300 Rorug07G0101800 Rorug07G0102000 Rorug07G0102100 Rorug07G0221200 Rorug07G0245700 Rorug07G0245700 Rorug07G0245700 Rorug07G0263900 Rorug07G0264000 Rorug07G0264000 Rorug07G0264500 Rorug07G0282900 Rorug07G0282900 Rorug07G0283100 Rorug07G0283200.1 Rorug07G0283300 Rorug07G0283900 Rorug07G0284000 Rorug07G0284100 Rorug07G0284300 Rorug07G0284400 Rorug07G0284500 Rorug07G0285000 Rorug07G0285200 Rorug07G0285300 Rorug07G0285500 Rorug07G0286800 Rorug07G0286900 Rorug07G0287100 Rorug07G0287200
rosa_samantha Rh7AG236100 Rh7AG236200 Rh7AG236300 Rh7AG440000 Rh7AG440100 Rh7BG411900 Rh7BG412000 Rh7BG412600 Rh7CG251300 Rh7CG417700 Rh7CG460100 Rh7DG242100 Rh7DG242200 Rh7DG242300 Rh7DG429300
rosa_wichuraiana Rw0G005070 Rw0G010780 Rw0G012530 Rw7G020110 Rw7G020160 Rw7G020170 Rw7G033210 Rw7G033230 Rw7G033250 Rw7G034540 Rw7G035150 Rw7G036190 Rw7G036210 Rw7G036260 Rw7G036270 Rw7G036290 Rw7G036300 Rw7G036310 Rw7G036350 Rw7G036360 Rw7G036440 Rw7G036510 Rw7G036530 Rw7G036570 Rw7G036590 Rw7G036730

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 2238
AccI GTMKAC 1 cut(s) 912
AccII CGCG 3 cut(s) 94, 600, 1249
AciI CCGC 8 cut(s) 249, 386, 675, 1249, 2033, 2238, 2532, 2749
AclWI GGATC 5 cut(s) 344, 643, 1392, 1554, 2762
AcoI YGGCCR 2 cut(s) 297, 468
AcsI RAATTY 4 cut(s) 660, 2014, 2271, 2435
AcuI CTGAAG 7 cut(s) 64, 221, 569, 1627, 1671, 1973, 2355
AfaI GTAC 6 cut(s) 62, 891, 1500, 1648, 1701, 2422
AfeI AGCGCT 2 cut(s) 413, 1532
AfiI CCNNNNNNNGG 5 cut(s) 363, 424, 1784, 2032, 2163
AflII CTTAAG 1 cut(s) 1277
AflIII ACRYGT 2 cut(s) 66, 1564
AjiI CACGTC 1 cut(s) 67
AjnI CCWGG 3 cut(s) 434, 2025, 2260
AjuI GAANNNNNNNTTGG 4 cut(s) 692, 724, 1211, 1243
Alw21I GWGCWC 3 cut(s) 1074, 2186, 2557
Alw26I GTCTC 3 cut(s) 394, 1132, 2529
Alw44I GTGCAC 2 cut(s) 2182, 2553
AlwI GGATC 5 cut(s) 344, 643, 1392, 1554, 2762
Aor51HI AGCGCT 2 cut(s) 413, 1532
AoxI GGCC 5 cut(s) 27, 297, 468, 1250, 2248
ApaLI GTGCAC 2 cut(s) 2182, 2553
ApeKI GCWGC 5 cut(s) 230, 458, 2198, 2711, 2826
ApoI RAATTY 4 cut(s) 660, 2014, 2271, 2435
AseI ATTAAT 2 cut(s) 2616, 2636
Asp700I GAANNNNTTC 4 cut(s) 393, 592, 1119, 1596
AspLEI GCGC 4 cut(s) 96, 414, 1533, 2653
AspS9I GGNCC 5 cut(s) 852, 922, 962, 1575, 2567
AsuC2I CCSGG 1 cut(s) 1514
AsuHPI GGTGA 2 cut(s) 2345, 2611
AvaII GGWCC 5 cut(s) 852, 922, 962, 1575, 2567
AxyI CCTNAGG 1 cut(s) 2301
BaeGI GKGCMC 2 cut(s) 2186, 2557
BalI TGGCCA 1 cut(s) 299
BanII GRGCYC 1 cut(s) 1074
BarI GAAGNNNNNNTAC 2 cut(s) 2202, 2234
BbsI GAAGAC 2 cut(s) 877, 1728
Bbv12I GWGCWC 3 cut(s) 1074, 2186, 2557
BbvI GCAGC 4 cut(s) 217, 445, 2185, 2723
BccI CCATC 5 cut(s) 19, 261, 1286, 1749, 2220
BceAI ACGGC 2 cut(s) 551, 2713
BciT130I CCWGG 3 cut(s) 436, 2027, 2262
BciVI GTATCC 1 cut(s) 1898
BclI TGATCA 1 cut(s) 1920
BcnI CCSGG 1 cut(s) 1514
BcoDI GTCTC 3 cut(s) 394, 1132, 2529
BfaI CTAG 7 cut(s) 222, 540, 1007, 1638, 1817, 2132, 2836
BfmI CTRYAG 3 cut(s) 459, 1534, 2219
BfoI RGCGCY 2 cut(s) 415, 1534
BfrI CTTAAG 1 cut(s) 1277
BfuI GTATCC 1 cut(s) 1898
BglII AGATCT 1 cut(s) 291
BisI GCNGC 6 cut(s) 231, 459, 1250, 2199, 2712, 2827
BlsI GCNGC 6 cut(s) 232, 460, 1251, 2200, 2713, 2828
BmcAI AGTACT 1 cut(s) 1648
Bme1390I CCNGG 4 cut(s) 436, 1514, 2027, 2262
Bme18I GGWCC 5 cut(s) 852, 922, 962, 1575, 2567
BmgBI CACGTC 1 cut(s) 67
BmgT120I GGNCC 5 cut(s) 852, 922, 962, 1575, 2567
BmiI GGNNCC 1 cut(s) 853
BmrFI CCNGG 4 cut(s) 436, 1514, 2027, 2262
BmrI ACTGGG 3 cut(s) 575, 689, 2766
BmsI GCATC 6 cut(s) 376, 433, 922, 1452, 1656, 2107
BmuI ACTGGG 3 cut(s) 575, 689, 2766
BoxI GACNNNNGTC 3 cut(s) 1833, 2503, 2761
BpiI GAAGAC 2 cut(s) 877, 1728
BpuEI CTTGAG 7 cut(s) 94, 229, 1088, 1114, 1790, 1898, 1976
BpuMI CCSGG 1 cut(s) 1514
Bsa29I ATCGAT 1 cut(s) 348
BsaAI YACGTR 1 cut(s) 1502
BsaBI GATNNNNATC 2 cut(s) 1803, 2589
BsaJI CCNNGG 4 cut(s) 855, 1512, 1522, 2260
BsaWI WCCGGW 1 cut(s) 1021
Bsc4I CCNNNNNNNGG 5 cut(s) 363, 424, 1784, 2032, 2163
Bse118I RCCGGY 1 cut(s) 470
Bse21I CCTNAGG 1 cut(s) 2301
Bse3DI GCAATG 3 cut(s) 1053, 2043, 2695
Bse8I GATNNNNATC 2 cut(s) 1803, 2589
BseBI CCWGG 3 cut(s) 436, 2027, 2262
BseCI ATCGAT 1 cut(s) 348
BseDI CCNNGG 4 cut(s) 855, 1512, 1522, 2260
BseGI GGATG 5 cut(s) 631, 1518, 2041, 2091, 2231
BseJI GATNNNNATC 2 cut(s) 1803, 2589
BseLI CCNNNNNNNGG 5 cut(s) 363, 424, 1784, 2032, 2163
BseMI GCAATG 3 cut(s) 1053, 2043, 2695
BseMII CTCAG 4 cut(s) 1823, 2315, 2608, 2750
BseRI GAGGAG 3 cut(s) 22, 25, 28
BseSI GKGCMC 2 cut(s) 2186, 2557
BseXI GCAGC 4 cut(s) 217, 445, 2185, 2723
BseYI CCCAGC 1 cut(s) 2494
BsgI GTGCAG 2 cut(s) 106, 449
Bsh1236I CGCG 3 cut(s) 94, 600, 1249
BshFI GGCC 5 cut(s) 29, 299, 470, 1252, 2250
BshVI ATCGAT 1 cut(s) 348
BsiHKAI GWGCWC 3 cut(s) 1074, 2186, 2557
BsiSI CCGG 4 cut(s) 471, 1022, 1259, 1514
BslFI GGGAC 4 cut(s) 865, 1198, 2511, 2716
BslI CCNNNNNNNGG 5 cut(s) 363, 424, 1784, 2032, 2163
BsmAI GTCTC 3 cut(s) 394, 1132, 2529
BsmFI GGGAC 4 cut(s) 865, 1198, 2511, 2716
BsmI GAATGC 2 cut(s) 2403, 2690
BsnI GGCC 5 cut(s) 29, 299, 470, 1252, 2250
Bsp1286I GDGCHC 3 cut(s) 1074, 2186, 2557
BspACI CCGC 8 cut(s) 249, 386, 675, 1249, 2033, 2238, 2532, 2749
BspANI GGCC 5 cut(s) 29, 299, 470, 1252, 2250
BspCNI CTCAG 4 cut(s) 1824, 2314, 2607, 2751
BspDI ATCGAT 1 cut(s) 348
BspFNI CGCG 3 cut(s) 94, 600, 1249
BspHI TCATGA 1 cut(s) 1371
BspLI GGNNCC 1 cut(s) 853
BspMAI CTGCAG 1 cut(s) 463
BspPI GGATC 5 cut(s) 344, 643, 1392, 1554, 2762
BspTI CTTAAG 1 cut(s) 1277
BsrBI CCGCTC 1 cut(s) 2238
BsrDI GCAATG 3 cut(s) 1053, 2043, 2695
BsrFI RCCGGY 1 cut(s) 470
BssAI RCCGGY 1 cut(s) 470
BssECI CCNNGG 4 cut(s) 855, 1512, 1522, 2260
BssT1I CCWWGG 1 cut(s) 1522
Bst2UI CCWGG 3 cut(s) 436, 2027, 2262
Bst4CI ACNGT 6 cut(s) 694, 1397, 1438, 1704, 2353, 2464
Bst6I CTCTTC 1 cut(s) 46
BstAFI CTTAAG 1 cut(s) 1277
BstBAI YACGTR 1 cut(s) 1502
BstC8I GCNNGC 9 cut(s) 207, 228, 301, 472, 602, 1086, 2236, 2457, 2557
BstDEI CTNAG 6 cut(s) 562, 1076, 1832, 2301, 2594, 2759
BstEII GGTNACC 1 cut(s) 614
BstF5I GGATG 5 cut(s) 631, 1518, 2041, 2091, 2231
BstFNI CGCG 3 cut(s) 94, 600, 1249
BstH2I RGCGCY 2 cut(s) 415, 1534
BstHHI GCGC 4 cut(s) 96, 414, 1533, 2653
BstMAI GTCTC 3 cut(s) 394, 1132, 2529
BstMWI GCNNNNNNNGC 6 cut(s) 84, 93, 467, 2000, 2400, 2832
BstNI CCWGG 3 cut(s) 436, 2027, 2262
BstPAI GACNNNNGTC 3 cut(s) 1833, 2503, 2761
BstPI GGTNACC 1 cut(s) 614
BstSCI CCNGG 4 cut(s) 434, 1512, 2025, 2260
BstSFI CTRYAG 3 cut(s) 459, 1534, 2219
BstSLI GKGCMC 2 cut(s) 2186, 2557
BstUI CGCG 3 cut(s) 94, 600, 1249
BstV1I GCAGC 4 cut(s) 217, 445, 2185, 2723
BstV2I GAAGAC 2 cut(s) 877, 1728
BstX2I RGATCY 1 cut(s) 291
BstXI CCANNNNNNTGG 1 cut(s) 2390
BstYI RGATCY 1 cut(s) 291
Bsu15I ATCGAT 1 cut(s) 348
Bsu36I CCTNAGG 1 cut(s) 2301
BsuI GTATCC 1 cut(s) 1898
BsuRI GGCC 5 cut(s) 29, 299, 470, 1252, 2250
BsuTUI ATCGAT 1 cut(s) 348
BtrI CACGTC 1 cut(s) 67
BtsCI GGATG 5 cut(s) 631, 1518, 2041, 2091, 2231
BtsI GCAGTG 2 cut(s) 1714, 2193
BtsIMutI CAGTG 9 cut(s) 197, 1163, 1482, 1707, 1714, 2193, 2460, 2577, 2779
Cac8I GCNNGC 9 cut(s) 207, 228, 301, 472, 602, 1086, 2236, 2457, 2557
CciI TCATGA 1 cut(s) 1371
CfoI GCGC 4 cut(s) 96, 414, 1533, 2653
Cfr10I RCCGGY 1 cut(s) 470
Cfr13I GGNCC 5 cut(s) 852, 922, 962, 1575, 2567
ClaI ATCGAT 1 cut(s) 348
Csp6I GTAC 6 cut(s) 61, 890, 1499, 1647, 1700, 2421
CviQI GTAC 6 cut(s) 61, 890, 1499, 1647, 1700, 2421
DdeI CTNAG 6 cut(s) 562, 1076, 1832, 2301, 2594, 2759
DraI TTTAAA 1 cut(s) 2340
EaeI YGGCCR 2 cut(s) 297, 468
Eam1104I CTCTTC 1 cut(s) 46
EarI CTCTTC 1 cut(s) 46
EciI GGCGGA 2 cut(s) 690, 2547
Ecl136II GAGCTC 1 cut(s) 1072
Eco130I CCWWGG 1 cut(s) 1522
Eco24I GRGCYC 1 cut(s) 1074
Eco32I GATATC 1 cut(s) 1825
Eco47I GGWCC 5 cut(s) 852, 922, 962, 1575, 2567
Eco47III AGCGCT 2 cut(s) 413, 1532
Eco53kI GAGCTC 1 cut(s) 1072
Eco57I CTGAAG 7 cut(s) 64, 221, 569, 1627, 1671, 1973, 2355
Eco81I CCTNAGG 1 cut(s) 2301
Eco91I GGTNACC 1 cut(s) 614
EcoICRI GAGCTC 1 cut(s) 1072
EcoO65I GGTNACC 1 cut(s) 614
EcoRII CCWGG 3 cut(s) 434, 2025, 2260
EcoRV GATATC 1 cut(s) 1825
EcoT14I CCWWGG 1 cut(s) 1522
EcoT22I ATGCAT 2 cut(s) 937, 1467
EcoT38I GRGCYC 1 cut(s) 1074
ErhI CCWWGG 1 cut(s) 1522
FalI AAGNNNNNCTT 6 cut(s) 432, 464, 1050, 1082, 1625, 1657
FaqI GGGAC 4 cut(s) 865, 1198, 2511, 2716
FauI CCCGC 2 cut(s) 242, 2756
FauNDI CATATG 2 cut(s) 640, 2172
FbaI TGATCA 1 cut(s) 1920
FblI GTMKAC 1 cut(s) 912
Fnu4HI GCNGC 6 cut(s) 231, 459, 1250, 2199, 2712, 2827
FokI GGATG 5 cut(s) 638, 1505, 2048, 2098, 2238
FriOI GRGCYC 1 cut(s) 1074
Fsp4HI GCNGC 6 cut(s) 231, 459, 1250, 2199, 2712, 2827
FspBI CTAG 7 cut(s) 222, 540, 1007, 1638, 1817, 2132, 2836
GlaI GCGC 4 cut(s) 95, 413, 1532, 2652
GluI GCNGC 6 cut(s) 231, 459, 1250, 2199, 2712, 2827
GsaI CCCAGC 1 cut(s) 2498
HaeII RGCGCY 2 cut(s) 415, 1534
HaeIII GGCC 5 cut(s) 29, 299, 470, 1252, 2250
HapII CCGG 4 cut(s) 471, 1022, 1259, 1514
HhaI GCGC 4 cut(s) 96, 414, 1533, 2653
Hin6I GCGC 4 cut(s) 94, 412, 1531, 2651
HinP1I GCGC 4 cut(s) 94, 412, 1531, 2651
HincII GTYRAC 1 cut(s) 529
HindII GTYRAC 1 cut(s) 529
HindIII AAGCTT 1 cut(s) 2516
HpaII CCGG 4 cut(s) 471, 1022, 1259, 1514
HphI GGTGA 2 cut(s) 2345, 2611
Hpy166II GTNNAC 7 cut(s) 529, 913, 922, 1267, 1707, 2184, 2555
Hpy8I GTNNAC 7 cut(s) 529, 913, 922, 1267, 1707, 2184, 2555
Hpy99I CGWCG 2 cut(s) 68, 161
HpyCH4III ACNGT 6 cut(s) 694, 1397, 1438, 1704, 2353, 2464
HpyCH4IV ACGT 7 cut(s) 66, 147, 949, 1501, 1564, 1627, 1869
HpyF10VI GCNNNNNNNGC 6 cut(s) 84, 93, 467, 2000, 2400, 2832
HpyF3I CTNAG 6 cut(s) 562, 1076, 1832, 2301, 2594, 2759
HpySE526I ACGT 7 cut(s) 66, 147, 949, 1501, 1564, 1627, 1869
HspAI GCGC 4 cut(s) 94, 412, 1531, 2651
KroI GCCGGC 1 cut(s) 470
KroNI GCCGGC 1 cut(s) 472
Ksp22I TGATCA 1 cut(s) 1920
LmnI GCTCC 1 cut(s) 1077
Lsp1109I GCAGC 4 cut(s) 217, 445, 2185, 2723
LweI GCATC 6 cut(s) 376, 433, 922, 1452, 1656, 2107
MaeI CTAG 7 cut(s) 222, 540, 1007, 1638, 1817, 2132, 2836
MaeII ACGT 7 cut(s) 66, 147, 949, 1501, 1564, 1627, 1869
MaeIII GTNAC 8 cut(s) 487, 614, 950, 1432, 1478, 1628, 1870, 2284
MbiI CCGCTC 1 cut(s) 2238
MfeI CAATTG 1 cut(s) 1912
MflI RGATCY 1 cut(s) 291
MhlI GDGCHC 3 cut(s) 1074, 2186, 2557
MlsI TGGCCA 1 cut(s) 299
MluNI TGGCCA 1 cut(s) 299
MlyI GAGTC 7 cut(s) 212, 269, 947, 1599, 1843, 1856, 2372
MmeI TCCRAC 2 cut(s) 565, 1890
Mox20I TGGCCA 1 cut(s) 299
Mph1103I ATGCAT 2 cut(s) 937, 1467
MroNI GCCGGC 1 cut(s) 470
MroXI GAANNNNTTC 4 cut(s) 393, 592, 1119, 1596
MscI TGGCCA 1 cut(s) 299
MslI CAYNNNNRTG 2 cut(s) 624, 1554
Msp20I TGGCCA 1 cut(s) 299
MspCI CTTAAG 1 cut(s) 1277
MspI CCGG 4 cut(s) 471, 1022, 1259, 1514
MspR9I CCNGG 4 cut(s) 436, 1514, 2027, 2262
MunI CAATTG 1 cut(s) 1912
Mva1269I GAATGC 2 cut(s) 2403, 2690
MvaI CCWGG 3 cut(s) 436, 2027, 2262
MvnI CGCG 3 cut(s) 94, 600, 1249
MwoI GCNNNNNNNGC 6 cut(s) 84, 93, 467, 2000, 2400, 2832
NaeI GCCGGC 1 cut(s) 472
NciI CCSGG 1 cut(s) 1514
NdeI CATATG 2 cut(s) 640, 2172
NgoMIV GCCGGC 1 cut(s) 470
NlaIV GGNNCC 1 cut(s) 853
NmeAIII GCCGAG 1 cut(s) 283
NmuCI GTSAC 5 cut(s) 487, 950, 1478, 1628, 1870
NsiI ATGCAT 2 cut(s) 937, 1467
PagI TCATGA 1 cut(s) 1371
PcsI WCGNNNNNNNCGW 3 cut(s) 153, 162, 354
PctI GAATGC 2 cut(s) 2403, 2690
PdiI GCCGGC 1 cut(s) 472
PdmI GAANNNNTTC 4 cut(s) 393, 592, 1119, 1596
PfeI GAWTC 8 cut(s) 141, 593, 782, 1061, 1474, 2104, 2167, 2740
PflFI GACNNNGTC 1 cut(s) 1367
PfoI TCCNGGA 1 cut(s) 2025
PkrI GCNGC 6 cut(s) 232, 460, 1251, 2200, 2713, 2828
PleI GAGTC 7 cut(s) 212, 268, 947, 1599, 1842, 1856, 2372
PpsI GAGTC 7 cut(s) 212, 268, 947, 1599, 1842, 1856, 2372
Ppu21I YACGTR 1 cut(s) 1502
PshAI GACNNNNGTC 3 cut(s) 1833, 2503, 2761
PshBI ATTAAT 2 cut(s) 2616, 2636
Psp124BI GAGCTC 1 cut(s) 1074
Psp6I CCWGG 3 cut(s) 434, 2025, 2260
PspEI GGTNACC 1 cut(s) 614
PspFI CCCAGC 1 cut(s) 2494
PspGI CCWGG 3 cut(s) 434, 2025, 2260
PspN4I GGNNCC 1 cut(s) 853
PspPI GGNCC 5 cut(s) 852, 922, 962, 1575, 2567
PsrI GAACNNNNNNTAC 2 cut(s) 53, 85
PstI CTGCAG 1 cut(s) 463
PsuI RGATCY 1 cut(s) 291
PsyI GACNNNGTC 1 cut(s) 1367
RsaI GTAC 6 cut(s) 62, 891, 1500, 1648, 1701, 2422
RsaNI GTAC 6 cut(s) 61, 890, 1499, 1647, 1700, 2421
RseI CAYNNNNRTG 2 cut(s) 624, 1554
SacI GAGCTC 1 cut(s) 1074
SatI GCNGC 6 cut(s) 231, 459, 1250, 2199, 2712, 2827
Sau96I GGNCC 5 cut(s) 852, 922, 962, 1575, 2567
ScaI AGTACT 1 cut(s) 1648
SchI GAGTC 7 cut(s) 212, 269, 947, 1599, 1843, 1856, 2372
ScrFI CCNGG 4 cut(s) 436, 1514, 2027, 2262
SduI GDGCHC 3 cut(s) 1074, 2186, 2557
SfaNI GCATC 6 cut(s) 376, 433, 922, 1452, 1656, 2107
SfcI CTRYAG 3 cut(s) 459, 1534, 2219
SinI GGWCC 5 cut(s) 852, 922, 962, 1575, 2567
SmiMI CAYNNNNRTG 2 cut(s) 624, 1554
SmlI CTYRAG 8 cut(s) 73, 244, 1067, 1129, 1277, 1769, 1877, 1955
SmoI CTYRAG 8 cut(s) 73, 244, 1067, 1129, 1277, 1769, 1877, 1955
SsiI CCGC 8 cut(s) 249, 386, 675, 1249, 2033, 2238, 2532, 2749
SspI AATATT 1 cut(s) 1148
SspMI CTAG 7 cut(s) 222, 540, 1007, 1638, 1817, 2132, 2836
SstI GAGCTC 1 cut(s) 1074
StyD4I CCNGG 4 cut(s) 434, 1512, 2025, 2260
StyI CCWWGG 1 cut(s) 1522
TaaI ACNGT 6 cut(s) 694, 1397, 1438, 1704, 2353, 2464
TaiI ACGT 7 cut(s) 69, 150, 952, 1504, 1567, 1630, 1872
TaqI TCGA 8 cut(s) 156, 263, 288, 348, 630, 1827, 2295, 2655
TaqII GACCGA 1 cut(s) 869
TatI WGTACW 1 cut(s) 1646
TauI GCSGC 1 cut(s) 1252
TfiI GAWTC 8 cut(s) 141, 593, 782, 1061, 1474, 2104, 2167, 2740
TscAI CASTG 9 cut(s) 204, 1170, 1489, 1714, 1721, 2200, 2467, 2577, 2779
TseFI GTSAC 5 cut(s) 487, 950, 1478, 1628, 1870
TseI GCWGC 5 cut(s) 230, 458, 2198, 2711, 2826
Tsp45I GTSAC 5 cut(s) 487, 950, 1478, 1628, 1870
TspGWI ACGGA 3 cut(s) 122, 1686, 2265
TspRI CASTG 9 cut(s) 204, 1170, 1489, 1714, 1721, 2200, 2467, 2577, 2779
Tth111I GACNNNGTC 1 cut(s) 1367
Vha464I CTTAAG 1 cut(s) 1277
VneI GTGCAC 2 cut(s) 2182, 2553
VpaK11BI GGWCC 5 cut(s) 852, 922, 962, 1575, 2567
VspI ATTAAT 2 cut(s) 2616, 2636
XapI RAATTY 4 cut(s) 660, 2014, 2271, 2435
XmiI GTMKAC 1 cut(s) 912
XmnI GAANNNNTTC 4 cut(s) 393, 592, 1119, 1596
XspI CTAG 7 cut(s) 222, 540, 1007, 1638, 1817, 2132, 2836
ZrmI AGTACT 1 cut(s) 1648
Zsp2I ATGCAT 2 cut(s) 937, 1467
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.