TMV resistance protein N-like
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 999 |
| AatII | GACGTC | 2 cut(s) | 639, 825 |
| Acc65I | GGTACC | 1 cut(s) | 1713 |
| AccB1I | GGYRCC | 1 cut(s) | 1713 |
| AccI | GTMKAC | 1 cut(s) | 1194 |
| AccII | CGCG | 1 cut(s) | 1215 |
| AciI | CCGC | 6 cut(s) | 234, 312, 496, 1137, 1445, 1597 |
| AclWI | GGATC | 4 cut(s) | 524, 635, 771, 1474 |
| AcsI | RAATTY | 5 cut(s) | 748, 948, 1542, 1939, 2257 |
| AcuI | CTGAAG | 3 cut(s) | 355, 759, 1860 |
| AcyI | GRCGYC | 2 cut(s) | 636, 822 |
| AfaI | GTAC | 6 cut(s) | 545, 1173, 1715, 1879, 1972, 2150 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 1146 |
| AflIII | ACRYGT | 2 cut(s) | 386, 1282 |
| AhlI | ACTAGT | 1 cut(s) | 1961 |
| AjnI | CCWGG | 2 cut(s) | 504, 1810 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 1493, 1525 |
| Alw21I | GWGCWC | 1 cut(s) | 1356 |
| Alw26I | GTCTC | 3 cut(s) | 569, 1952, 1990 |
| AlwI | GGATC | 4 cut(s) | 524, 635, 771, 1474 |
| Ama87I | CYCGRG | 3 cut(s) | 566, 885, 1246 |
| AoxI | GGCC | 3 cut(s) | 403, 429, 1243 |
| ApeKI | GCWGC | 4 cut(s) | 728, 1127, 1207, 2154 |
| ApoI | RAATTY | 5 cut(s) | 748, 948, 1542, 1939, 2257 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 2154, 2186 |
| AseI | ATTAAT | 1 cut(s) | 1728 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 1155 |
| Asp718I | GGTACC | 1 cut(s) | 1713 |
| AspLEI | GCGC | 1 cut(s) | 862 |
| AspS9I | GGNCC | 5 cut(s) | 403, 1134, 1244, 1333, 1699 |
| AsuC2I | CCSGG | 2 cut(s) | 1247, 1248 |
| AsuHPI | GGTGA | 2 cut(s) | 273, 1333 |
| AsuII | TTCGAA | 1 cut(s) | 746 |
| AvaI | CYCGRG | 3 cut(s) | 566, 885, 1246 |
| AvaII | GGWCC | 3 cut(s) | 1134, 1333, 1699 |
| BaeI | ACNNNNGTAYC | 4 cut(s) | 873, 906, 2032, 2065 |
| BanI | GGYRCC | 1 cut(s) | 1713 |
| BanII | GRGCYC | 1 cut(s) | 1356 |
| BauI | CACGAG | 1 cut(s) | 1533 |
| BbsI | GAAGAC | 2 cut(s) | 387, 523 |
| Bbv12I | GWGCWC | 1 cut(s) | 1356 |
| BbvI | GCAGC | 4 cut(s) | 715, 1114, 1219, 2166 |
| BccI | CCATC | 4 cut(s) | 706, 1658, 2061, 2330 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 1765, 1799 |
| BciT130I | CCWGG | 2 cut(s) | 506, 1812 |
| BciVI | GTATCC | 1 cut(s) | 2033 |
| BcnI | CCSGG | 2 cut(s) | 1247, 1248 |
| BcoDI | GTCTC | 3 cut(s) | 569, 1952, 1990 |
| BcuI | ACTAGT | 1 cut(s) | 1961 |
| BfaI | CTAG | 7 cut(s) | 513, 605, 893, 1061, 1272, 1392, 1962 |
| BfmI | CTRYAG | 1 cut(s) | 1128 |
| BfoI | RGCGCY | 1 cut(s) | 863 |
| BfuI | GTATCC | 1 cut(s) | 2033 |
| BisI | GCNGC | 5 cut(s) | 729, 1128, 1208, 1445, 2155 |
| BlsI | GCNGC | 5 cut(s) | 730, 1129, 1209, 1446, 2156 |
| BmcAI | AGTACT | 2 cut(s) | 1879, 1972 |
| Bme1390I | CCNGG | 4 cut(s) | 506, 1247, 1248, 1812 |
| Bme18I | GGWCC | 3 cut(s) | 1134, 1333, 1699 |
| BmeT110I | CYCGRG | 3 cut(s) | 566, 885, 1246 |
| BmgT120I | GGNCC | 5 cut(s) | 403, 1134, 1244, 1333, 1699 |
| BmiI | GGNNCC | 2 cut(s) | 1135, 1715 |
| BmrFI | CCNGG | 4 cut(s) | 506, 1247, 1248, 1812 |
| BmsI | GCATC | 3 cut(s) | 924, 1740, 1843 |
| BpiI | GAAGAC | 2 cut(s) | 387, 523 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 715, 747 |
| BpmI | CTGGAG | 2 cut(s) | 538, 1833 |
| Bpu10I | CCTNAGC | 1 cut(s) | 19 |
| Bpu14I | TTCGAA | 1 cut(s) | 746 |
| BpuEI | CTTGAG | 3 cut(s) | 520, 1370, 2291 |
| BpuMI | CCSGG | 2 cut(s) | 1247, 1248 |
| Bsa29I | ATCGAT | 1 cut(s) | 2332 |
| BsaAI | YACGTR | 1 cut(s) | 389 |
| BsaBI | GATNNNNATC | 1 cut(s) | 521 |
| BsaHI | GRCGYC | 2 cut(s) | 636, 822 |
| BsaJI | CCNNGG | 5 cut(s) | 505, 1140, 1246, 1247, 1810 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 536, 566 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 1146 |
| Bse1I | ACTGG | 4 cut(s) | 14, 670, 1228, 1715 |
| Bse8I | GATNNNNATC | 1 cut(s) | 521 |
| BseBI | CCWGG | 2 cut(s) | 506, 1812 |
| BseCI | ATCGAT | 1 cut(s) | 2332 |
| BseDI | CCNNGG | 5 cut(s) | 505, 1140, 1246, 1247, 1810 |
| BseGI | GGATG | 8 cut(s) | 206, 245, 269, 293, 813, 1519, 1669, 1834 |
| BseJI | GATNNNNATC | 1 cut(s) | 521 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 1146 |
| BseMII | CTCAG | 4 cut(s) | 781, 1991, 2173, 2230 |
| BseNI | ACTGG | 4 cut(s) | 14, 670, 1228, 1715 |
| BseRI | GAGGAG | 9 cut(s) | 101, 211, 220, 241, 250, 268, 298, 483, 584 |
| BseXI | GCAGC | 4 cut(s) | 715, 1114, 1219, 2166 |
| BseYI | CCCAGC | 2 cut(s) | 759, 2033 |
| Bsh1236I | CGCG | 1 cut(s) | 1215 |
| BshFI | GGCC | 3 cut(s) | 405, 431, 1245 |
| BshNI | GGYRCC | 1 cut(s) | 1713 |
| BshVI | ATCGAT | 1 cut(s) | 2332 |
| BsiHKAI | GWGCWC | 1 cut(s) | 1356 |
| BsiHKCI | CYCGRG | 3 cut(s) | 566, 885, 1246 |
| BsiSI | CCGG | 2 cut(s) | 432, 1247 |
| BslFI | GGGAC | 2 cut(s) | 140, 1147 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 1146 |
| BsmAI | GTCTC | 3 cut(s) | 569, 1952, 1990 |
| BsmFI | GGGAC | 2 cut(s) | 140, 1147 |
| BsnI | GGCC | 3 cut(s) | 405, 431, 1245 |
| BsoBI | CYCGRG | 3 cut(s) | 566, 885, 1246 |
| Bsp119I | TTCGAA | 1 cut(s) | 746 |
| Bsp1286I | GDGCHC | 1 cut(s) | 1356 |
| Bsp143I | GATC | 8 cut(s) | 516, 582, 640, 763, 1466, 2230, 2329, 2333 |
| BspACI | CCGC | 6 cut(s) | 234, 312, 496, 1137, 1445, 1597 |
| BspANI | GGCC | 3 cut(s) | 405, 431, 1245 |
| BspCNI | CTCAG | 4 cut(s) | 780, 1992, 2172, 2229 |
| BspDI | ATCGAT | 1 cut(s) | 2332 |
| BspFNI | CGCG | 1 cut(s) | 1215 |
| BspLI | GGNNCC | 2 cut(s) | 1135, 1715 |
| BspMAI | CTGCAG | 1 cut(s) | 1132 |
| BspPI | GGATC | 4 cut(s) | 524, 635, 771, 1474 |
| BspT104I | TTCGAA | 1 cut(s) | 746 |
| BspT107I | GGYRCC | 1 cut(s) | 1713 |
| BsrI | ACTGG | 4 cut(s) | 14, 670, 1228, 1715 |
| BssECI | CCNNGG | 5 cut(s) | 505, 1140, 1246, 1247, 1810 |
| BssMI | GATC | 8 cut(s) | 516, 582, 640, 763, 1466, 2230, 2329, 2333 |
| BssNI | GRCGYC | 2 cut(s) | 636, 822 |
| BssSI | CACGAG | 1 cut(s) | 1533 |
| BssT1I | CCWWGG | 1 cut(s) | 1140 |
| Bst2BI | CACGAG | 1 cut(s) | 1533 |
| Bst2UI | CCWGG | 2 cut(s) | 506, 1812 |
| Bst4CI | ACNGT | 5 cut(s) | 54, 543, 1433, 2115, 2294 |
| Bst6I | CTCTTC | 2 cut(s) | 460, 1988 |
| BstACI | GRCGYC | 2 cut(s) | 636, 822 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 1759 |
| BstBAI | YACGTR | 1 cut(s) | 389 |
| BstBI | TTCGAA | 1 cut(s) | 746 |
| BstC8I | GCNNGC | 7 cut(s) | 347, 498, 1368, 1449, 1791, 2031, 2078 |
| BstDEI | CTNAG | 8 cut(s) | 19, 461, 767, 1358, 1974, 2000, 2159, 2216 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 1144 |
| BstF5I | GGATG | 8 cut(s) | 206, 245, 269, 293, 813, 1519, 1669, 1834 |
| BstFNI | CGCG | 1 cut(s) | 1215 |
| BstH2I | RGCGCY | 1 cut(s) | 863 |
| BstHHI | GCGC | 1 cut(s) | 862 |
| BstKTI | GATC | 8 cut(s) | 519, 585, 643, 766, 1469, 2233, 2332, 2336 |
| BstMAI | GTCTC | 3 cut(s) | 569, 1952, 1990 |
| BstMBI | GATC | 8 cut(s) | 516, 582, 640, 763, 1466, 2230, 2329, 2333 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 375, 1136, 1251, 1759 |
| BstNI | CCWGG | 2 cut(s) | 506, 1812 |
| BstNSI | RCATGY | 4 cut(s) | 653, 1370, 1793, 2146 |
| BstSCI | CCNGG | 4 cut(s) | 504, 1245, 1246, 1810 |
| BstSFI | CTRYAG | 1 cut(s) | 1128 |
| BstUI | CGCG | 1 cut(s) | 1215 |
| BstV1I | GCAGC | 4 cut(s) | 715, 1114, 1219, 2166 |
| BstV2I | GAAGAC | 2 cut(s) | 387, 523 |
| BstX2I | RGATCY | 3 cut(s) | 516, 763, 1466 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 1241 |
| BstYI | RGATCY | 3 cut(s) | 516, 763, 1466 |
| Bsu15I | ATCGAT | 1 cut(s) | 2332 |
| BsuI | GTATCC | 1 cut(s) | 2033 |
| BsuRI | GGCC | 3 cut(s) | 405, 431, 1245 |
| BsuTUI | ATCGAT | 1 cut(s) | 2332 |
| BtsCI | GGATG | 8 cut(s) | 206, 245, 269, 293, 813, 1519, 1669, 1834 |
| BtsI | GCAGTG | 1 cut(s) | 1126 |
| BtsIMutI | CAGTG | 1 cut(s) | 1126 |
| Cac8I | GCNNGC | 7 cut(s) | 347, 498, 1368, 1449, 1791, 2031, 2078 |
| CfoI | GCGC | 1 cut(s) | 862 |
| Cfr13I | GGNCC | 5 cut(s) | 403, 1134, 1244, 1333, 1699 |
| Cfr9I | CCCGGG | 1 cut(s) | 1246 |
| ClaI | ATCGAT | 1 cut(s) | 2332 |
| Csp6I | GTAC | 6 cut(s) | 544, 1172, 1714, 1878, 1971, 2149 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 1414, 1449 |
| CviAII | CATG | 9 cut(s) | 586, 650, 788, 958, 1367, 1754, 1790, 2081, 2143 |
| CviQI | GTAC | 6 cut(s) | 544, 1172, 1714, 1878, 1971, 2149 |
| DdeI | CTNAG | 8 cut(s) | 19, 461, 767, 1358, 1974, 2000, 2159, 2216 |
| DpnI | GATC | 8 cut(s) | 518, 584, 642, 765, 1468, 2232, 2331, 2335 |
| DpnII | GATC | 8 cut(s) | 516, 582, 640, 763, 1466, 2230, 2329, 2333 |
| Eam1104I | CTCTTC | 2 cut(s) | 460, 1988 |
| EarI | CTCTTC | 2 cut(s) | 460, 1988 |
| EciI | GGCGGA | 3 cut(s) | 223, 301, 1612 |
| Ecl136II | GAGCTC | 1 cut(s) | 1354 |
| Eco130I | CCWWGG | 1 cut(s) | 1140 |
| Eco24I | GRGCYC | 1 cut(s) | 1356 |
| Eco32I | GATATC | 2 cut(s) | 1093, 1783 |
| Eco47I | GGWCC | 3 cut(s) | 1134, 1333, 1699 |
| Eco53kI | GAGCTC | 1 cut(s) | 1354 |
| Eco57I | CTGAAG | 3 cut(s) | 355, 759, 1860 |
| Eco88I | CYCGRG | 3 cut(s) | 566, 885, 1246 |
| EcoICRI | GAGCTC | 1 cut(s) | 1354 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 1144 |
| EcoO109I | RGGNCCY | 1 cut(s) | 1699 |
| EcoRII | CCWGG | 2 cut(s) | 504, 1810 |
| EcoRV | GATATC | 2 cut(s) | 1093, 1783 |
| EcoT14I | CCWWGG | 1 cut(s) | 1140 |
| EcoT22I | ATGCAT | 1 cut(s) | 1755 |
| EcoT38I | GRGCYC | 1 cut(s) | 1356 |
| ErhI | CCWWGG | 1 cut(s) | 1140 |
| FaeI | CATG | 9 cut(s) | 589, 653, 791, 961, 1370, 1757, 1793, 2084, 2146 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 1332, 1364 |
| FaqI | GGGAC | 2 cut(s) | 140, 1147 |
| FatI | CATG | 9 cut(s) | 585, 649, 787, 957, 1366, 1753, 1789, 2080, 2142 |
| FauI | CCCGC | 1 cut(s) | 489 |
| FauNDI | CATATG | 1 cut(s) | 928 |
| FblI | GTMKAC | 1 cut(s) | 1194 |
| Fnu4HI | GCNGC | 5 cut(s) | 729, 1128, 1208, 1445, 2155 |
| FokI | GGATG | 8 cut(s) | 193, 232, 256, 280, 820, 1526, 1676, 1821 |
| FriOI | GRGCYC | 1 cut(s) | 1356 |
| Fsp4HI | GCNGC | 5 cut(s) | 729, 1128, 1208, 1445, 2155 |
| FspBI | CTAG | 7 cut(s) | 513, 605, 893, 1061, 1272, 1392, 1962 |
| GlaI | GCGC | 1 cut(s) | 861 |
| GluI | GCNGC | 5 cut(s) | 729, 1128, 1208, 1445, 2155 |
| GsaI | CCCAGC | 2 cut(s) | 763, 2037 |
| GsuI | CTGGAG | 2 cut(s) | 538, 1833 |
| HaeII | RGCGCY | 1 cut(s) | 863 |
| HaeIII | GGCC | 3 cut(s) | 405, 431, 1245 |
| HapII | CCGG | 2 cut(s) | 432, 1247 |
| HhaI | GCGC | 1 cut(s) | 862 |
| Hin1I | GRCGYC | 2 cut(s) | 636, 822 |
| Hin1II | CATG | 9 cut(s) | 589, 653, 791, 961, 1370, 1757, 1793, 2084, 2146 |
| Hin6I | GCGC | 1 cut(s) | 860 |
| HinP1I | GCGC | 1 cut(s) | 860 |
| HincII | GTYRAC | 1 cut(s) | 2120 |
| HindII | GTYRAC | 1 cut(s) | 2120 |
| HindIII | AAGCTT | 2 cut(s) | 367, 741 |
| HpaII | CCGG | 2 cut(s) | 432, 1247 |
| HphI | GGTGA | 2 cut(s) | 273, 1333 |
| Hpy166II | GTNNAC | 3 cut(s) | 1195, 2120, 2314 |
| Hpy8I | GTNNAC | 3 cut(s) | 1195, 2120, 2314 |
| Hpy99I | CGWCG | 4 cut(s) | 458, 641, 857, 1574 |
| HpyAV | CCTTC | 4 cut(s) | 343, 654, 1016, 1919 |
| HpyCH4III | ACNGT | 5 cut(s) | 54, 543, 1433, 2115, 2294 |
| HpyCH4IV | ACGT | 7 cut(s) | 388, 636, 822, 855, 1282, 1906, 2022 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 375, 1136, 1251, 1759 |
| HpyF3I | CTNAG | 8 cut(s) | 19, 461, 767, 1358, 1974, 2000, 2159, 2216 |
| HpySE526I | ACGT | 7 cut(s) | 388, 636, 822, 855, 1282, 1906, 2022 |
| Hsp92I | GRCGYC | 2 cut(s) | 636, 822 |
| Hsp92II | CATG | 9 cut(s) | 589, 653, 791, 961, 1370, 1757, 1793, 2084, 2146 |
| HspAI | GCGC | 1 cut(s) | 860 |
| KpnI | GGTACC | 1 cut(s) | 1717 |
| Kzo9I | GATC | 8 cut(s) | 516, 582, 640, 763, 1466, 2230, 2329, 2333 |
| LmnI | GCTCC | 4 cut(s) | 571, 1251, 1359, 2013 |
| Lsp1109I | GCAGC | 4 cut(s) | 715, 1114, 1219, 2166 |
| LweI | GCATC | 3 cut(s) | 924, 1740, 1843 |
| MaeI | CTAG | 7 cut(s) | 513, 605, 893, 1061, 1272, 1392, 1962 |
| MaeII | ACGT | 7 cut(s) | 388, 636, 822, 855, 1282, 1906, 2022 |
| MaeIII | GTNAC | 3 cut(s) | 48, 823, 1720 |
| MalI | GATC | 8 cut(s) | 518, 584, 642, 765, 1468, 2232, 2331, 2335 |
| MboI | GATC | 8 cut(s) | 516, 582, 640, 763, 1466, 2230, 2329, 2333 |
| MboII | GAAGA | 9 cut(s) | 97, 392, 477, 485, 523, 609, 1352, 1853, 2005 |
| MflI | RGATCY | 3 cut(s) | 516, 763, 1466 |
| MhlI | GDGCHC | 1 cut(s) | 1356 |
| MlyI | GAGTC | 2 cut(s) | 545, 2349 |
| MmeI | TCCRAC | 3 cut(s) | 573, 853, 2035 |
| Mph1103I | ATGCAT | 1 cut(s) | 1755 |
| MroXI | GAANNNNTTC | 1 cut(s) | 1155 |
| MslI | CAYNNNNRTG | 1 cut(s) | 699 |
| MspA1I | CMGCKG | 2 cut(s) | 558, 1447 |
| MspI | CCGG | 2 cut(s) | 432, 1247 |
| MspR9I | CCNGG | 4 cut(s) | 506, 1247, 1248, 1812 |
| MvaI | CCWGG | 2 cut(s) | 506, 1812 |
| MvnI | CGCG | 1 cut(s) | 1215 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 375, 1136, 1251, 1759 |
| NciI | CCSGG | 2 cut(s) | 1247, 1248 |
| NdeI | CATATG | 1 cut(s) | 928 |
| NdeII | GATC | 8 cut(s) | 516, 582, 640, 763, 1466, 2230, 2329, 2333 |
| NlaIII | CATG | 9 cut(s) | 589, 653, 791, 961, 1370, 1757, 1793, 2084, 2146 |
| NlaIV | GGNNCC | 2 cut(s) | 1135, 1715 |
| NmuCI | GTSAC | 1 cut(s) | 823 |
| NsiI | ATGCAT | 1 cut(s) | 1755 |
| NspI | RCATGY | 4 cut(s) | 653, 1370, 1793, 2146 |
| NspV | TTCGAA | 1 cut(s) | 746 |
| PaeI | GCATGC | 2 cut(s) | 1370, 1793 |
| PaeR7I | CTCGAG | 2 cut(s) | 566, 885 |
| PdmI | GAANNNNTTC | 1 cut(s) | 1155 |
| PfeI | GAWTC | 8 cut(s) | 435, 923, 1257, 1528, 1692, 2085, 2206, 2212 |
| PkrI | GCNGC | 5 cut(s) | 730, 1129, 1209, 1446, 2156 |
| PleI | GAGTC | 2 cut(s) | 545, 2348 |
| PpsI | GAGTC | 2 cut(s) | 545, 2348 |
| Ppu21I | YACGTR | 1 cut(s) | 389 |
| PpuMI | RGGWCCY | 1 cut(s) | 1699 |
| PshBI | ATTAAT | 1 cut(s) | 1728 |
| PsiI | TTATAA | 1 cut(s) | 999 |
| Psp124BI | GAGCTC | 1 cut(s) | 1356 |
| Psp5II | RGGWCCY | 1 cut(s) | 1699 |
| Psp6I | CCWGG | 2 cut(s) | 504, 1810 |
| PspFI | CCCAGC | 2 cut(s) | 759, 2033 |
| PspGI | CCWGG | 2 cut(s) | 504, 1810 |
| PspN4I | GGNNCC | 2 cut(s) | 1135, 1715 |
| PspPI | GGNCC | 5 cut(s) | 403, 1134, 1244, 1333, 1699 |
| PspPPI | RGGWCCY | 1 cut(s) | 1699 |
| PspXI | VCTCGAGB | 1 cut(s) | 885 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 830, 862 |
| PstI | CTGCAG | 1 cut(s) | 1132 |
| PsuI | RGATCY | 3 cut(s) | 516, 763, 1466 |
| PvuII | CAGCTG | 1 cut(s) | 558 |
| RsaI | GTAC | 6 cut(s) | 545, 1173, 1715, 1879, 1972, 2150 |
| RsaNI | GTAC | 6 cut(s) | 544, 1172, 1714, 1878, 1971, 2149 |
| RseI | CAYNNNNRTG | 1 cut(s) | 699 |
| SacI | GAGCTC | 1 cut(s) | 1356 |
| SatI | GCNGC | 5 cut(s) | 729, 1128, 1208, 1445, 2155 |
| Sau3AI | GATC | 8 cut(s) | 516, 582, 640, 763, 1466, 2230, 2329, 2333 |
| Sau96I | GGNCC | 5 cut(s) | 403, 1134, 1244, 1333, 1699 |
| ScaI | AGTACT | 2 cut(s) | 1879, 1972 |
| SchI | GAGTC | 2 cut(s) | 545, 2349 |
| ScrFI | CCNGG | 4 cut(s) | 506, 1247, 1248, 1812 |
| SduI | GDGCHC | 1 cut(s) | 1356 |
| SfaNI | GCATC | 3 cut(s) | 924, 1740, 1843 |
| SfcI | CTRYAG | 1 cut(s) | 1128 |
| Sfr274I | CTCGAG | 2 cut(s) | 566, 885 |
| SfuI | TTCGAA | 1 cut(s) | 746 |
| SinI | GGWCC | 3 cut(s) | 1134, 1333, 1699 |
| SlaI | CTCGAG | 2 cut(s) | 566, 885 |
| SmaI | CCCGGG | 1 cut(s) | 1248 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 699 |
| SmlI | CTYRAG | 5 cut(s) | 535, 566, 885, 1349, 2270 |
| SmoI | CTYRAG | 5 cut(s) | 535, 566, 885, 1349, 2270 |
| SpeI | ACTAGT | 1 cut(s) | 1961 |
| SphI | GCATGC | 2 cut(s) | 1370, 1793 |
| SsiI | CCGC | 6 cut(s) | 234, 312, 496, 1137, 1445, 1597 |
| SspI | AATATT | 1 cut(s) | 627 |
| SspMI | CTAG | 7 cut(s) | 513, 605, 893, 1061, 1272, 1392, 1962 |
| SstI | GAGCTC | 1 cut(s) | 1356 |
| StyD4I | CCNGG | 4 cut(s) | 504, 1245, 1246, 1810 |
| StyI | CCWWGG | 1 cut(s) | 1140 |
| TaaI | ACNGT | 5 cut(s) | 54, 543, 1433, 2115, 2294 |
| TaiI | ACGT | 7 cut(s) | 391, 639, 825, 858, 1285, 1909, 2025 |
| TaqII | GACCGA | 1 cut(s) | 71 |
| TatI | WGTACW | 3 cut(s) | 1877, 1970, 2148 |
| TauI | GCSGC | 1 cut(s) | 1447 |
| TfiI | GAWTC | 8 cut(s) | 435, 923, 1257, 1528, 1692, 2085, 2206, 2212 |
| TscAI | CASTG | 1 cut(s) | 1126 |
| TseFI | GTSAC | 1 cut(s) | 823 |
| TseI | GCWGC | 4 cut(s) | 728, 1127, 1207, 2154 |
| Tsp45I | GTSAC | 1 cut(s) | 823 |
| TspGWI | ACGGA | 2 cut(s) | 142, 1834 |
| TspMI | CCCGGG | 1 cut(s) | 1246 |
| TspRI | CASTG | 1 cut(s) | 1126 |
| VpaK11BI | GGWCC | 3 cut(s) | 1134, 1333, 1699 |
| VspI | ATTAAT | 1 cut(s) | 1728 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 1144 |
| XapI | RAATTY | 5 cut(s) | 748, 948, 1542, 1939, 2257 |
| XceI | RCATGY | 4 cut(s) | 653, 1370, 1793, 2146 |
| XhoI | CTCGAG | 2 cut(s) | 566, 885 |
| XmaI | CCCGGG | 1 cut(s) | 1246 |
| XmiI | GTMKAC | 1 cut(s) | 1194 |
| XmnI | GAANNNNTTC | 1 cut(s) | 1155 |
| XspI | CTAG | 7 cut(s) | 513, 605, 893, 1061, 1272, 1392, 1962 |
| ZraI | GACGTC | 2 cut(s) | 637, 823 |
| ZrmI | AGTACT | 2 cut(s) | 1879, 1972 |
| Zsp2I | ATGCAT | 1 cut(s) | 1755 |