Rroxscaffold_6G00425110

Ribosomal protein L12

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0004878
Physical Location & Seq
Reverse (-)
46111597 .. 46113551
1955 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00425110.1

Sequence Viewer

Length: 339 bp
ATGTTCGAGAAGATCACATTTGATATAAAGCTTGAGAAGTTTGACGCGGCAGCAAAGATCAAAATCATAAAGGAAGGTAGGACTTTCACTTGTTTGGAAGTAGAGGACGCTAAAGACTTGGTGGAAAAAGCTCATGTTGTGCTTGAAGAAGGGTGTGACCAAAGAGGTGGCAGGCCTATTGTTGACAAGCTCAAGAAGTTGGGTGCTATACTTGCTATGTGTATCAAGAACCCCGAGATCCCCATCCTTATCGGCCGGCACCTTGTACGATCCTCTATAGCGACCCACATCTTCACCCATTACACATACGGTGGGGTCCCTAGCCATCTCTTCTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L12

Protein Analysis

112

Amino Acids

12.62

Weight (kDa)

8.45

Isoelectric Point (pI)

36.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L12 PF00542 7 - 70 7.8e-12 Ribosomal protein L7/L12 C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 258
AccII CGCG 1 cut(s) 47
AciI CCGC 1 cut(s) 47
AclWI GGATC 2 cut(s) 232, 264
AcoI YGGCCR 1 cut(s) 253
AfaI GTAC 1 cut(s) 267
AgsI TTSAA 1 cut(s) 146
AluBI AGCT 3 cut(s) 31, 131, 190
AluI AGCT 3 cut(s) 31, 131, 190
AlwI GGATC 2 cut(s) 232, 264
Ama87I CYCGRG 1 cut(s) 233
AoxI GGCC 2 cut(s) 173, 253
ApeKI GCWGC 1 cut(s) 50
AspS9I GGNCC 1 cut(s) 316
AsuHPI GGTGA 1 cut(s) 286
AvaI CYCGRG 1 cut(s) 233
AvaII GGWCC 1 cut(s) 316
BanI GGYRCC 1 cut(s) 258
BbvI GCAGC 1 cut(s) 62
BccI CCATC 2 cut(s) 251, 333
BfaI CTAG 1 cut(s) 321
BfmI CTRYAG 1 cut(s) 276
BisI GCNGC 2 cut(s) 48, 51
BlsI GCNGC 2 cut(s) 49, 52
Bme18I GGWCC 1 cut(s) 316
BmeT110I CYCGRG 1 cut(s) 233
BmgT120I GGNCC 1 cut(s) 316
BmiI GGNNCC 3 cut(s) 260, 317, 318
BpuEI CTTGAG 2 cut(s) 53, 176
BsaBI GATNNNNATC 2 cut(s) 62, 242
Bse118I RCCGGY 1 cut(s) 255
Bse8I GATNNNNATC 2 cut(s) 62, 242
BseGI GGATG 1 cut(s) 243
BseJI GATNNNNATC 2 cut(s) 62, 242
BseX3I CGGCCG 1 cut(s) 253
BseXI GCAGC 1 cut(s) 62
Bsh1236I CGCG 1 cut(s) 47
Bsh1285I CGRYCG 1 cut(s) 256
BshFI GGCC 2 cut(s) 175, 255
BshNI GGYRCC 1 cut(s) 258
BsiEI CGRYCG 1 cut(s) 256
BsiHKCI CYCGRG 1 cut(s) 233
BsiSI CCGG 1 cut(s) 256
BslFI GGGAC 1 cut(s) 302
BsmFI GGGAC 1 cut(s) 302
BsnI GGCC 2 cut(s) 175, 255
BsoBI CYCGRG 1 cut(s) 233
Bsp143I GATC 4 cut(s) 12, 57, 237, 269
BspACI CCGC 1 cut(s) 47
BspANI GGCC 2 cut(s) 175, 255
BspFNI CGCG 1 cut(s) 47
BspLI GGNNCC 3 cut(s) 260, 317, 318
BspPI GGATC 2 cut(s) 232, 264
BspT107I GGYRCC 1 cut(s) 258
BsrFI RCCGGY 1 cut(s) 255
BssAI RCCGGY 1 cut(s) 255
BssMI GATC 4 cut(s) 12, 57, 237, 269
Bst4CI ACNGT 1 cut(s) 311
Bst6I CTCTTC 1 cut(s) 335
BstC8I GCNNGC 2 cut(s) 173, 257
BstF5I GGATG 1 cut(s) 243
BstFNI CGCG 1 cut(s) 47
BstKTI GATC 4 cut(s) 15, 60, 240, 272
BstMBI GATC 4 cut(s) 12, 57, 237, 269
BstMCI CGRYCG 1 cut(s) 256
BstMWI GCNNNNNNNGC 1 cut(s) 212
BstSFI CTRYAG 1 cut(s) 276
BstUI CGCG 1 cut(s) 47
BstV1I GCAGC 1 cut(s) 62
BstX2I RGATCY 1 cut(s) 237
BstXI CCANNNNNNTGG 1 cut(s) 167
BstYI RGATCY 1 cut(s) 237
BstZI CGGCCG 1 cut(s) 253
BsuRI GGCC 2 cut(s) 175, 255
BtsCI GGATG 1 cut(s) 243
Cac8I GCNNGC 2 cut(s) 173, 257
Cfr10I RCCGGY 1 cut(s) 255
Cfr13I GGNCC 1 cut(s) 316
CseI GACGC 2 cut(s) 53, 116
Csp6I GTAC 1 cut(s) 266
CviAII CATG 1 cut(s) 134
CviJI RGCY 6 cut(s) 31, 131, 175, 190, 255, 324
CviKI_1 RGCY 6 cut(s) 31, 131, 175, 190, 255, 324
CviQI GTAC 1 cut(s) 266
DpnI GATC 4 cut(s) 14, 59, 239, 271
DpnII GATC 4 cut(s) 12, 57, 237, 269
EaeI YGGCCR 1 cut(s) 253
EagI CGGCCG 1 cut(s) 253
Eam1104I CTCTTC 1 cut(s) 335
EarI CTCTTC 1 cut(s) 335
EclXI CGGCCG 1 cut(s) 253
Eco147I AGGCCT 1 cut(s) 175
Eco47I GGWCC 1 cut(s) 316
Eco52I CGGCCG 1 cut(s) 253
Eco88I CYCGRG 1 cut(s) 233
EcoO109I RGGNCCY 1 cut(s) 316
FaeI CATG 1 cut(s) 137
FaiI YATR 7 cut(s) 26, 68, 135, 209, 218, 278, 307
FaqI GGGAC 1 cut(s) 302
FatI CATG 1 cut(s) 133
Fnu4HI GCNGC 2 cut(s) 48, 51
FokI GGATG 1 cut(s) 230
Fsp4HI GCNGC 2 cut(s) 48, 51
FspBI CTAG 1 cut(s) 321
GluI GCNGC 2 cut(s) 48, 51
HaeIII GGCC 2 cut(s) 175, 255
HapII CCGG 1 cut(s) 256
HgaI GACGC 2 cut(s) 53, 116
Hin1II CATG 1 cut(s) 137
HincII GTYRAC 1 cut(s) 184
HindII GTYRAC 1 cut(s) 184
HindIII AAGCTT 1 cut(s) 29
HpaII CCGG 1 cut(s) 256
HphI GGTGA 1 cut(s) 286
Hpy166II GTNNAC 1 cut(s) 184
Hpy188III TCNNGA 3 cut(s) 7, 193, 226
Hpy8I GTNNAC 1 cut(s) 184
HpyAV CCTTC 2 cut(s) 68, 143
HpyCH4III ACNGT 1 cut(s) 311
HpyF10VI GCNNNNNNNGC 1 cut(s) 212
Hsp92II CATG 1 cut(s) 137
KflI GGGWCCC 1 cut(s) 316
KroI GCCGGC 1 cut(s) 255
KroNI GCCGGC 1 cut(s) 257
Kzo9I GATC 4 cut(s) 12, 57, 237, 269
LpnPI CCDG 2 cut(s) 157, 269
Lsp1109I GCAGC 1 cut(s) 62
MaeI CTAG 1 cut(s) 321
MaeIII GTNAC 1 cut(s) 155
MalI GATC 4 cut(s) 14, 59, 239, 271
MboI GATC 4 cut(s) 12, 57, 237, 269
MboII GAAGA 5 cut(s) 22, 158, 283, 322, 325
MflI RGATCY 1 cut(s) 237
MnlI CCTC 3 cut(s) 97, 158, 283
MroNI GCCGGC 1 cut(s) 255
MspI CCGG 1 cut(s) 256
MvnI CGCG 1 cut(s) 47
MwoI GCNNNNNNNGC 1 cut(s) 212
NaeI GCCGGC 1 cut(s) 257
NdeII GATC 4 cut(s) 12, 57, 237, 269
NgoMIV GCCGGC 1 cut(s) 255
NlaIII CATG 1 cut(s) 137
NlaIV GGNNCC 3 cut(s) 260, 317, 318
NmuCI GTSAC 1 cut(s) 155
PceI AGGCCT 1 cut(s) 175
PdiI GCCGGC 1 cut(s) 257
PkrI GCNGC 2 cut(s) 49, 52
PpuMI RGGWCCY 1 cut(s) 316
Psp5II RGGWCCY 1 cut(s) 316
PspN4I GGNNCC 3 cut(s) 260, 317, 318
PspPI GGNCC 1 cut(s) 316
PspPPI RGGWCCY 1 cut(s) 316
PsuI RGATCY 1 cut(s) 237
RsaI GTAC 1 cut(s) 267
RsaNI GTAC 1 cut(s) 266
SatI GCNGC 2 cut(s) 48, 51
Sau3AI GATC 4 cut(s) 12, 57, 237, 269
Sau96I GGNCC 1 cut(s) 316
SetI ASST 6 cut(s) 33, 79, 133, 169, 192, 264
SfcI CTRYAG 1 cut(s) 276
SinI GGWCC 1 cut(s) 316
SmlI CTYRAG 2 cut(s) 32, 191
SmoI CTYRAG 2 cut(s) 32, 191
SseBI AGGCCT 1 cut(s) 175
SsiI CCGC 1 cut(s) 47
SspMI CTAG 1 cut(s) 321
StuI AGGCCT 1 cut(s) 175
TaaI ACNGT 1 cut(s) 311
TaqI TCGA 1 cut(s) 6
TauI GCSGC 1 cut(s) 50
TseFI GTSAC 1 cut(s) 155
TseI GCWGC 1 cut(s) 50
Tsp45I GTSAC 1 cut(s) 155
VpaK11BI GGWCC 1 cut(s) 316
XspI CTAG 1 cut(s) 321
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.