Rorug07G0313500

Ribosomal protein L12

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0004878
Physical Location & Seq
Forward (+)
31485197 .. 31494815
9619 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0313500.1

Sequence Viewer

Length: 1311 bp
ATGGCAGATAATGTGTCAGCTAGACCTACTGTGCGTGAGACTGTGCCAGCAATGCCACTGACAGATAATGCATTTGCTACCCCTACTGTGCGTGAAAATGTGCTAGCAACAACTACTGTTCATGCTCATGTCAGTCATGTACGAGCAAGAGCTAACACGCCACCAAGAGCTAATTTGCCACCACAAACTAATGTGCCACTACCAGCTAACGTGGATCCATCAATTCATCAGCTGCCTACCCAACAAGAAATCCCTTTTGTGGGCATTGGCATGAAAAGGAAGCGTGGTAAATCATGTGGTAAGGCTCTTCAGGAGCTTATAAAGGCTAATGGTGGACCTCTGCGTGTTGACTTTCATCGGACCATCCATGTCCTTTCTGATGACACAATTTCTAAAATGTTTACTTCTGAGATAGGAATTACTGTTCGAGGTGGGGCACCAGTATGTAAATATGGCTGGTCTGATATTGATGAAGAAGATGATAAGAGGTTGCTAAGAGAGGAATTGCGGGTGTTCTTTATGGTGGACTTAACTGATCCAGTTGTTGTTGCGTATGTGGATTCCAAAATGTCTACTGCCTACTCCCAATTCAAGACTCGACTAAAGAAGGAATGGAAAGCATTTGGCTCACCTGAGCTAGGAAGAGCAAACCTGCCTAGTGCAGATTTATGGAATGAGAGACCTGTCTCACATTGGCACTGGCTTTGTGACAACATATACACCAACCAGTCTTGCATTGAGATTGCGGAAAAGAATTCTGCTAACAGGTATATGCAACAACATACTCACAGAGCTGGTGCACGGCCATACGTGCAACATGCTTTGGTGGCCTCTAAGGGTGGGAAGGAGTTGTCACTCATACAGAATTGGGGAGAGAAACACAAGGATAATAATCATGAATGGATCAATGAGGCTGCCAAGAACAAATATGAGAAGATGGAGGCTGAGAGGAATGCCCTTTTAGAGAAGATGTACCAAGAGGCACCTGAGGGTACTGCACATGATTCTATCAAACTGACTTTGGAAGTTGAGTACCCAATAATGGCCAAGAAGCTTGGGAGGAAGGGTAGGATGATCCATGGCATTGGTAACGTCCCTCACATAAGTTCTAACATACGTAGAGTATCTGCATGGGGTGGCAACAATTCTGAGGTAGCTGAATTGCGTGATCTTATCGACCAACTTACCTTTAACATGCTCAATATGAAGGAACAAAATGGACTATTGAAGGCTCAAATGGAATATTTATTGAGGCAATCTCCTGGGCAGATGAGTGAGGATGACTTCCCCCATCCTAGCACCAACTGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L12

Protein Analysis

436

Amino Acids

49.15

Weight (kDa)

8.44

Isoelectric Point (pI)

48.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Transposase_24 PF03004 231 - 355 1.2e-08 Plant transposase (Ptta/En/Spm family)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 320
Acc36I ACCTGC 1 cut(s) 660
AccB1I GGYRCC 2 cut(s) 436, 982
AccI GTMKAC 1 cut(s) 572
AciI CCGC 2 cut(s) 508, 746
AclWI GGATC 5 cut(s) 209, 222, 530, 911, 1069
AcoI YGGCCR 2 cut(s) 803, 1044
AcsI RAATTY 1 cut(s) 754
AcuI CTGAAG 1 cut(s) 293
AfaI GTAC 4 cut(s) 141, 974, 994, 1034
AfiI CCNNNNNNNGG 4 cut(s) 259, 260, 638, 1042
AgsI TTSAA 2 cut(s) 592, 1228
AjnI CCWGG 1 cut(s) 1261
AloI GAACNNNNNNTCC 2 cut(s) 408, 440
Alw21I GWGCWC 1 cut(s) 802
Alw26I GTCTC 3 cut(s) 32, 673, 691
Alw44I GTGCAC 1 cut(s) 798
AlwI GGATC 5 cut(s) 209, 222, 530, 911, 1069
AoxI GGCC 3 cut(s) 803, 828, 1044
ApaLI GTGCAC 1 cut(s) 798
ApeKI GCWGC 2 cut(s) 232, 914
ApoI RAATTY 1 cut(s) 754
AspS9I GGNCC 2 cut(s) 335, 360
AsuHPI GGTGA 1 cut(s) 621
AsuNHI GCTAGC 1 cut(s) 103
AvaII GGWCC 2 cut(s) 335, 360
AxyI CCTNAGG 1 cut(s) 987
BaeGI GKGCMC 2 cut(s) 439, 802
BalI TGGCCA 1 cut(s) 1046
BamHI GGATCC 1 cut(s) 214
BanI GGYRCC 2 cut(s) 436, 982
Bbv12I GWGCWC 1 cut(s) 802
BbvI GCAGC 2 cut(s) 219, 901
BccI CCATC 4 cut(s) 226, 371, 931, 1299
BceAI ACGGC 1 cut(s) 818
BciT130I CCWGG 1 cut(s) 1263
BcoDI GTCTC 3 cut(s) 32, 673, 691
BfaI CTAG 5 cut(s) 21, 104, 638, 657, 1296
BfuAI ACCTGC 1 cut(s) 660
BisI GCNGC 2 cut(s) 233, 915
BlsI GCNGC 2 cut(s) 234, 916
Bme1390I CCNGG 1 cut(s) 1263
Bme18I GGWCC 2 cut(s) 335, 360
BmgT120I GGNCC 2 cut(s) 335, 360
BmiI GGNNCC 3 cut(s) 216, 438, 984
BmrFI CCNGG 1 cut(s) 1263
BmtI GCTAGC 1 cut(s) 107
BplI GAGNNNNNCTC 2 cut(s) 1243, 1275
Bpu10I CCTNAGC 1 cut(s) 633
BsaAI YACGTR 2 cut(s) 811, 1118
BsaBI GATNNNNATC 1 cut(s) 891
BsaI GGTCTC 1 cut(s) 673
BsaJI CCNNGG 2 cut(s) 1078, 1262
BsaXI ACNNNNNCTCC 2 cut(s) 1051, 1081
Bsc4I CCNNNNNNNGG 4 cut(s) 259, 260, 638, 1042
Bse1I ACTGG 4 cut(s) 440, 539, 704, 727
Bse21I CCTNAGG 1 cut(s) 987
Bse3DI GCAATG 1 cut(s) 57
Bse8I GATNNNNATC 1 cut(s) 891
BseBI CCWGG 1 cut(s) 1263
BseDI CCNNGG 2 cut(s) 1078, 1262
BseGI GGATG 4 cut(s) 363, 1077, 1285, 1291
BseJI GATNNNNATC 1 cut(s) 891
BseLI CCNNNNNNNGG 4 cut(s) 259, 260, 638, 1042
BseMI GCAATG 1 cut(s) 57
BseMII CTCAG 5 cut(s) 399, 624, 936, 978, 1140
BseNI ACTGG 4 cut(s) 440, 539, 704, 727
BseSI GKGCMC 2 cut(s) 439, 802
BseXI GCAGC 2 cut(s) 219, 901
BsgI GTGCAG 2 cut(s) 681, 981
BshFI GGCC 3 cut(s) 805, 830, 1046
BshNI GGYRCC 2 cut(s) 436, 982
BsiHKAI GWGCWC 1 cut(s) 802
BslFI GGGAC 1 cut(s) 1079
BslI CCNNNNNNNGG 4 cut(s) 259, 260, 638, 1042
BsmAI GTCTC 3 cut(s) 32, 673, 691
BsmFI GGGAC 1 cut(s) 1079
BsmI GAATGC 1 cut(s) 958
BsnI GGCC 3 cut(s) 805, 830, 1046
Bso31I GGTCTC 1 cut(s) 673
Bsp1286I GDGCHC 2 cut(s) 439, 802
Bsp143I GATC 5 cut(s) 214, 535, 903, 1074, 1168
Bsp19I CCATGG 1 cut(s) 1078
BspACI CCGC 2 cut(s) 508, 746
BspANI GGCC 3 cut(s) 805, 830, 1046
BspCNI CTCAG 5 cut(s) 400, 625, 937, 979, 1141
BspHI TCATGA 1 cut(s) 895
BspLI GGNNCC 3 cut(s) 216, 438, 984
BspMI ACCTGC 1 cut(s) 660
BspOI GCTAGC 1 cut(s) 107
BspPI GGATC 5 cut(s) 209, 222, 530, 911, 1069
BspQI GCTCTTC 2 cut(s) 312, 637
BspT107I GGYRCC 2 cut(s) 436, 982
BspTNI GGTCTC 1 cut(s) 673
BsrDI GCAATG 1 cut(s) 57
BsrI ACTGG 4 cut(s) 440, 539, 704, 727
BssECI CCNNGG 2 cut(s) 1078, 1262
BssMI GATC 5 cut(s) 214, 535, 903, 1074, 1168
BssT1I CCWWGG 1 cut(s) 1078
Bst2UI CCWGG 1 cut(s) 1263
Bst4CI ACNGT 5 cut(s) 31, 43, 88, 118, 424
Bst6I CTCTTC 2 cut(s) 312, 637
BstAPI GCANNNNNTGC 1 cut(s) 1305
BstBAI YACGTR 2 cut(s) 811, 1118
BstC8I GCNNGC 2 cut(s) 48, 105
BstDEI CTNAG 7 cut(s) 408, 494, 633, 834, 945, 987, 1149
BstDSI CCRYGG 1 cut(s) 1078
BstENI CCTNNNNNAGG 1 cut(s) 636
BstF5I GGATG 4 cut(s) 363, 1077, 1285, 1291
BstKTI GATC 5 cut(s) 217, 538, 906, 1077, 1171
BstMAI GTCTC 3 cut(s) 32, 673, 691
BstMBI GATC 5 cut(s) 214, 535, 903, 1074, 1168
BstMWI GCNNNNNNNGC 4 cut(s) 52, 811, 827, 1305
BstNI CCWGG 1 cut(s) 1263
BstNSI RCATGY 2 cut(s) 821, 1198
BstSCI CCNGG 1 cut(s) 1261
BstSLI GKGCMC 2 cut(s) 439, 802
BstSNI TACGTA 1 cut(s) 1118
BstV1I GCAGC 2 cut(s) 219, 901
BstX2I RGATCY 1 cut(s) 214
BstXI CCANNNNNNTGG 1 cut(s) 1085
BstYI RGATCY 1 cut(s) 214
Bsu36I CCTNAGG 1 cut(s) 987
BsuRI GGCC 3 cut(s) 805, 830, 1046
BtgI CCRYGG 1 cut(s) 1078
BtsCI GGATG 4 cut(s) 363, 1077, 1285, 1291
BtsIMutI CAGTG 2 cut(s) 56, 697
BveI ACCTGC 1 cut(s) 660
Cac8I GCNNGC 2 cut(s) 48, 105
CciI TCATGA 1 cut(s) 895
Cfr13I GGNCC 2 cut(s) 335, 360
Csp6I GTAC 4 cut(s) 140, 973, 993, 1033
CviQI GTAC 4 cut(s) 140, 973, 993, 1033
DdeI CTNAG 7 cut(s) 408, 494, 633, 834, 945, 987, 1149
DpnI GATC 5 cut(s) 216, 537, 905, 1076, 1170
DpnII GATC 5 cut(s) 214, 535, 903, 1074, 1168
EaeI YGGCCR 2 cut(s) 803, 1044
Eam1104I CTCTTC 2 cut(s) 312, 637
EarI CTCTTC 2 cut(s) 312, 637
Eco105I TACGTA 1 cut(s) 1118
Eco130I CCWWGG 1 cut(s) 1078
Eco31I GGTCTC 1 cut(s) 673
Eco47I GGWCC 2 cut(s) 335, 360
Eco57I CTGAAG 1 cut(s) 293
Eco81I CCTNAGG 1 cut(s) 987
EcoNI CCTNNNNNAGG 1 cut(s) 636
EcoRI GAATTC 1 cut(s) 754
EcoRII CCWGG 1 cut(s) 1261
EcoT14I CCWWGG 1 cut(s) 1078
EcoT22I ATGCAT 1 cut(s) 73
ErhI CCWWGG 1 cut(s) 1078
FaqI GGGAC 1 cut(s) 1079
FauI CCCGC 1 cut(s) 501
FblI GTMKAC 1 cut(s) 572
Fnu4HI GCNGC 2 cut(s) 233, 915
FokI GGATG 4 cut(s) 350, 1084, 1278, 1292
Fsp4HI GCNGC 2 cut(s) 233, 915
FspBI CTAG 5 cut(s) 21, 104, 638, 657, 1296
GluI GCNGC 2 cut(s) 233, 915
HaeIII GGCC 3 cut(s) 805, 830, 1046
HincII GTYRAC 1 cut(s) 349
HindII GTYRAC 1 cut(s) 349
HindIII AAGCTT 1 cut(s) 1052
HinfI GANTC 3 cut(s) 560, 595, 1004
HphI GGTGA 1 cut(s) 621
Hpy166II GTNNAC 6 cut(s) 335, 349, 402, 526, 573, 800
Hpy188I TCNGA 5 cut(s) 360, 379, 409, 463, 1150
Hpy188III TCNNGA 3 cut(s) 311, 592, 896
Hpy8I GTNNAC 6 cut(s) 335, 349, 402, 526, 573, 800
HpyAV CCTTC 5 cut(s) 601, 838, 1057, 1201, 1222
HpyCH4III ACNGT 5 cut(s) 31, 43, 88, 118, 424
HpyCH4IV ACGT 4 cut(s) 210, 810, 1092, 1117
HpyCH4V TGCA 8 cut(s) 71, 662, 735, 775, 800, 814, 998, 1130
HpyF10VI GCNNNNNNNGC 4 cut(s) 52, 811, 827, 1305
HpyF3I CTNAG 7 cut(s) 408, 494, 633, 834, 945, 987, 1149
HpySE526I ACGT 4 cut(s) 210, 810, 1092, 1117
Kzo9I GATC 5 cut(s) 214, 535, 903, 1074, 1168
LguI GCTCTTC 2 cut(s) 312, 637
LmnI GCTCC 1 cut(s) 313
Lsp1109I GCAGC 2 cut(s) 219, 901
MaeI CTAG 5 cut(s) 21, 104, 638, 657, 1296
MaeII ACGT 4 cut(s) 210, 810, 1092, 1117
MaeIII GTNAC 3 cut(s) 707, 852, 1088
MalI GATC 5 cut(s) 216, 537, 905, 1076, 1170
MboI GATC 5 cut(s) 214, 535, 903, 1074, 1168
MboII GAAGA 6 cut(s) 299, 485, 488, 654, 946, 979
MflI RGATCY 1 cut(s) 214
MhlI GDGCHC 2 cut(s) 439, 802
MlsI TGGCCA 1 cut(s) 1046
MluNI TGGCCA 1 cut(s) 1046
MlyI GAGTC 1 cut(s) 589
Mox20I TGGCCA 1 cut(s) 1046
Mph1103I ATGCAT 1 cut(s) 73
MscI TGGCCA 1 cut(s) 1046
MseI TTAA 2 cut(s) 530, 1191
MslI CAYNNNNRTG 3 cut(s) 126, 269, 442
Msp20I TGGCCA 1 cut(s) 1046
MspA1I CMGCKG 1 cut(s) 232
MspR9I CCNGG 1 cut(s) 1263
Mva1269I GAATGC 1 cut(s) 958
MvaI CCWGG 1 cut(s) 1263
MwoI GCNNNNNNNGC 4 cut(s) 52, 811, 827, 1305
NcoI CCATGG 1 cut(s) 1078
NdeII GATC 5 cut(s) 214, 535, 903, 1074, 1168
NheI GCTAGC 1 cut(s) 103
NlaIV GGNNCC 3 cut(s) 216, 438, 984
NmuCI GTSAC 2 cut(s) 707, 852
NsiI ATGCAT 1 cut(s) 73
NspI RCATGY 2 cut(s) 821, 1198
PagI TCATGA 1 cut(s) 895
PciSI GCTCTTC 2 cut(s) 312, 637
PctI GAATGC 1 cut(s) 958
PfeI GAWTC 2 cut(s) 560, 1004
PkrI GCNGC 2 cut(s) 234, 916
PleI GAGTC 1 cut(s) 589
PpsI GAGTC 1 cut(s) 589
Ppu21I YACGTR 2 cut(s) 811, 1118
PsiI TTATAA 1 cut(s) 320
Psp6I CCWGG 1 cut(s) 1261
PspGI CCWGG 1 cut(s) 1261
PspN4I GGNNCC 3 cut(s) 216, 438, 984
PspPI GGNCC 2 cut(s) 335, 360
PsuI RGATCY 1 cut(s) 214
PvuII CAGCTG 1 cut(s) 232
RsaI GTAC 4 cut(s) 141, 974, 994, 1034
RsaNI GTAC 4 cut(s) 140, 973, 993, 1033
RseI CAYNNNNRTG 3 cut(s) 126, 269, 442
SapI GCTCTTC 2 cut(s) 312, 637
SaqAI TTAA 2 cut(s) 530, 1191
SatI GCNGC 2 cut(s) 233, 915
Sau3AI GATC 5 cut(s) 214, 535, 903, 1074, 1168
Sau96I GGNCC 2 cut(s) 335, 360
SchI GAGTC 1 cut(s) 589
ScrFI CCNGG 1 cut(s) 1263
SduI GDGCHC 2 cut(s) 439, 802
SinI GGWCC 2 cut(s) 335, 360
SmiMI CAYNNNNRTG 3 cut(s) 126, 269, 442
SnaBI TACGTA 1 cut(s) 1118
SsiI CCGC 2 cut(s) 508, 746
SspI AATATT 1 cut(s) 1244
SspMI CTAG 5 cut(s) 21, 104, 638, 657, 1296
StyD4I CCNGG 1 cut(s) 1261
StyI CCWWGG 1 cut(s) 1078
TaaI ACNGT 5 cut(s) 31, 43, 88, 118, 424
TaiI ACGT 4 cut(s) 213, 813, 1095, 1120
TaqI TCGA 3 cut(s) 427, 598, 1176
TfiI GAWTC 2 cut(s) 560, 1004
Tru1I TTAA 2 cut(s) 530, 1191
Tru9I TTAA 2 cut(s) 530, 1191
TscAI CASTG 2 cut(s) 63, 704
TseFI GTSAC 2 cut(s) 707, 852
TseI GCWGC 2 cut(s) 232, 914
Tsp45I GTSAC 2 cut(s) 707, 852
TspDTI ATGAA 7 cut(s) 110, 215, 287, 344, 486, 912, 1220
TspRI CASTG 2 cut(s) 63, 704
VneI GTGCAC 1 cut(s) 798
VpaK11BI GGWCC 2 cut(s) 335, 360
XagI CCTNNNNNAGG 1 cut(s) 636
XapI RAATTY 1 cut(s) 754
XceI RCATGY 2 cut(s) 821, 1198
XmiI GTMKAC 1 cut(s) 572
XspI CTAG 5 cut(s) 21, 104, 638, 657, 1296
Zsp2I ATGCAT 1 cut(s) 73
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.