Rroxscaffold_7G00161210

Late embryogenesis abundant protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0002155
Physical Location & Seq
Forward (+)
4017656 .. 4018117
462 bp
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UTR
Exon/CDS
Intron
Rroxscaffold_7G00161210.1

Sequence Viewer

Length: 462 bp
ATGGTAAGGGTTCTTCATCGCGACTCATACGACGAACCGATAAACCCTCAAGGCGTGGGCTGTTGTATGCTCATATTCGTCAGCCTGATGACCACGCTTCTGCTGATCGACACAGTTGTACATCAGTCGCTCAATCCAGAGGAACCCCAGTTCTGGGTCGACTCAGCTGCCGTGACCGGACTCGAGCTCACAGGCTCCTCCGCATTGACTGCCACGTGGAACATGACGCTCGTGGCACACAATCCCAACCGCAAGCTCGTGATCGACATCGCCGGTGTCCAAGTGTTCATATATTTCGGAAAAATGGAGAAGTACTACTGGTACAACCATCGGCATCATGATCTGGTTGAGAGCCAACACATTCTGGCGACGAAGCTGTTGCCTTCTTTCTTCTTGTACAGAGGAAACCAGACGAGTCTGGATTTCAAGGTCGGAGTAGCGGGGCATTGCGTCGACGGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LEA_2

Protein Analysis

153

Amino Acids

17.26

Weight (kDa)

6.01

Isoelectric Point (pI)

26.97

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 159, 453
AccII CGCG 1 cut(s) 21
AciI CCGC 3 cut(s) 201, 250, 440
AcvI CACGTG 1 cut(s) 216
AfaI GTAC 4 cut(s) 120, 314, 323, 398
AfiI CCNNNNNNNGG 2 cut(s) 153, 154
AgsI TTSAA 1 cut(s) 427
AloI GAACNNNNNNTCC 2 cut(s) 134, 166
AluBI AGCT 4 cut(s) 167, 187, 256, 376
AluI AGCT 4 cut(s) 167, 187, 256, 376
Alw21I GWGCWC 1 cut(s) 189
Ama87I CYCGRG 1 cut(s) 182
ApeKI GCWGC 1 cut(s) 167
AvaI CYCGRG 1 cut(s) 182
BanII GRGCYC 1 cut(s) 189
BarI GAAGNNNNNNTAC 1 cut(s) 29
BauI CACGAG 2 cut(s) 230, 257
BbrPI CACGTG 1 cut(s) 216
Bbv12I GWGCWC 1 cut(s) 189
BbvI GCAGC 1 cut(s) 154
BccI CCATC 1 cut(s) 336
BceAI ACGGC 1 cut(s) 155
BcgI CGANNNNNNTGC 4 cut(s) 149, 183, 361, 395
BfaI CTAG 1 cut(s) 460
BisI GCNGC 1 cut(s) 168
BlsI GCNGC 1 cut(s) 169
BmcAI AGTACT 1 cut(s) 314
BmeT110I CYCGRG 1 cut(s) 182
BmiI GGNNCC 2 cut(s) 144, 196
BmrI ACTGGG 1 cut(s) 142
BmsI GCATC 1 cut(s) 343
BmuI ACTGGG 1 cut(s) 142
BpuEI CTTGAG 1 cut(s) 33
BsaAI YACGTR 1 cut(s) 216
BsaBI GATNNNNATC 1 cut(s) 266
BsaWI WCCGGW 1 cut(s) 176
Bsc4I CCNNNNNNNGG 2 cut(s) 153, 154
Bse118I RCCGGY 1 cut(s) 272
Bse1I ACTGG 2 cut(s) 148, 323
Bse3DI GCAATG 1 cut(s) 445
Bse8I GATNNNNATC 1 cut(s) 266
BseJI GATNNNNATC 1 cut(s) 266
BseLI CCNNNNNNNGG 2 cut(s) 153, 154
BseMI GCAATG 1 cut(s) 445
BseMII CTCAG 1 cut(s) 177
BseNI ACTGG 2 cut(s) 148, 323
BseRI GAGGAG 1 cut(s) 187
BseXI GCAGC 1 cut(s) 154
Bsh1236I CGCG 1 cut(s) 21
BsiHKAI GWGCWC 1 cut(s) 189
BsiHKCI CYCGRG 1 cut(s) 182
BsiSI CCGG 2 cut(s) 177, 273
BslI CCNNNNNNNGG 2 cut(s) 153, 154
BsoBI CYCGRG 1 cut(s) 182
Bsp1286I GDGCHC 1 cut(s) 189
Bsp1407I TGTACA 2 cut(s) 118, 396
Bsp143I GATC 3 cut(s) 105, 261, 340
Bsp68I TCGCGA 1 cut(s) 21
BspACI CCGC 3 cut(s) 201, 250, 440
BspCNI CTCAG 1 cut(s) 176
BspFNI CGCG 1 cut(s) 21
BspHI TCATGA 1 cut(s) 337
BspLI GGNNCC 2 cut(s) 144, 196
BsrDI GCAATG 1 cut(s) 445
BsrFI RCCGGY 1 cut(s) 272
BsrGI TGTACA 2 cut(s) 118, 396
BsrI ACTGG 2 cut(s) 148, 323
BssAI RCCGGY 1 cut(s) 272
BssMI GATC 3 cut(s) 105, 261, 340
BssSI CACGAG 2 cut(s) 230, 257
Bst2BI CACGAG 2 cut(s) 230, 257
Bst4CI ACNGT 1 cut(s) 115
BstAPI GCANNNNNTGC 1 cut(s) 209
BstAUI TGTACA 2 cut(s) 118, 396
BstBAI YACGTR 1 cut(s) 216
BstC8I GCNNGC 1 cut(s) 254
BstDEI CTNAG 1 cut(s) 163
BstFNI CGCG 1 cut(s) 21
BstKTI GATC 3 cut(s) 108, 264, 343
BstMBI GATC 3 cut(s) 105, 261, 340
BstMWI GCNNNNNNNGC 2 cut(s) 209, 456
BstUI CGCG 1 cut(s) 21
BstV1I GCAGC 1 cut(s) 154
BtgZI GCGATG 2 cut(s) 2, 253
BtuMI TCGCGA 1 cut(s) 21
Cac8I GCNNGC 1 cut(s) 254
CciI TCATGA 1 cut(s) 337
Cfr10I RCCGGY 1 cut(s) 272
CseI GACGC 2 cut(s) 235, 439
Csp6I GTAC 4 cut(s) 119, 313, 322, 397
CviAII CATG 2 cut(s) 223, 338
CviJI RGCY 9 cut(s) 60, 84, 167, 187, 195, 256, 354, 376, 459
CviKI_1 RGCY 9 cut(s) 60, 84, 167, 187, 195, 256, 354, 376, 459
CviQI GTAC 4 cut(s) 119, 313, 322, 397
DdeI CTNAG 1 cut(s) 163
DpnI GATC 3 cut(s) 107, 263, 342
DpnII GATC 3 cut(s) 105, 261, 340
Ecl136II GAGCTC 1 cut(s) 187
Eco24I GRGCYC 1 cut(s) 189
Eco53kI GAGCTC 1 cut(s) 187
Eco72I CACGTG 1 cut(s) 216
Eco88I CYCGRG 1 cut(s) 182
EcoICRI GAGCTC 1 cut(s) 187
EcoT38I GRGCYC 1 cut(s) 189
FaeI CATG 2 cut(s) 226, 341
FaiI YATR 7 cut(s) 28, 68, 74, 224, 290, 292, 339
FatI CATG 2 cut(s) 222, 337
FauI CCCGC 1 cut(s) 433
FblI GTMKAC 2 cut(s) 159, 453
Fnu4HI GCNGC 1 cut(s) 168
FriOI GRGCYC 1 cut(s) 189
Fsp4HI GCNGC 1 cut(s) 168
FspBI CTAG 1 cut(s) 460
GluI GCNGC 1 cut(s) 168
HapII CCGG 2 cut(s) 177, 273
HgaI GACGC 2 cut(s) 235, 439
Hin1II CATG 2 cut(s) 226, 341
HincII GTYRAC 2 cut(s) 160, 454
HindII GTYRAC 2 cut(s) 160, 454
HinfI GANTC 4 cut(s) 23, 161, 180, 415
HpaII CCGG 2 cut(s) 177, 273
Hpy166II GTNNAC 2 cut(s) 160, 454
Hpy188I TCNGA 2 cut(s) 299, 434
Hpy188III TCNNGA 5 cut(s) 20, 137, 259, 338, 419
Hpy8I GTNNAC 2 cut(s) 160, 454
Hpy99I CGWCG 4 cut(s) 35, 373, 455, 458
HpyAV CCTTC 1 cut(s) 393
HpyCH4III ACNGT 1 cut(s) 115
HpyCH4IV ACGT 1 cut(s) 215
HpyF10VI GCNNNNNNNGC 2 cut(s) 209, 456
HpyF3I CTNAG 1 cut(s) 163
HpySE526I ACGT 1 cut(s) 215
Hsp92II CATG 2 cut(s) 226, 341
Kzo9I GATC 3 cut(s) 105, 261, 340
LmnI GCTCC 1 cut(s) 200
Lsp1109I GCAGC 1 cut(s) 154
LweI GCATC 1 cut(s) 343
MaeI CTAG 1 cut(s) 460
MaeII ACGT 1 cut(s) 215
MaeIII GTNAC 1 cut(s) 172
MalI GATC 3 cut(s) 107, 263, 342
MboI GATC 3 cut(s) 105, 261, 340
MboII GAAGA 2 cut(s) 5, 382
MhlI GDGCHC 1 cut(s) 189
MlyI GAGTC 4 cut(s) 17, 155, 174, 424
MmeI TCCRAC 1 cut(s) 412
MnlI CCTC 4 cut(s) 57, 133, 208, 395
MspA1I CMGCKG 1 cut(s) 167
MspI CCGG 2 cut(s) 177, 273
MvnI CGCG 1 cut(s) 21
MwoI GCNNNNNNNGC 2 cut(s) 209, 456
NdeII GATC 3 cut(s) 105, 261, 340
NlaIII CATG 2 cut(s) 226, 341
NlaIV GGNNCC 2 cut(s) 144, 196
NmuCI GTSAC 1 cut(s) 172
NruI TCGCGA 1 cut(s) 21
PaeR7I CTCGAG 1 cut(s) 182
PagI TCATGA 1 cut(s) 337
PkrI GCNGC 1 cut(s) 169
PleI GAGTC 4 cut(s) 17, 155, 174, 423
PmaCI CACGTG 1 cut(s) 216
PmlI CACGTG 1 cut(s) 216
PpsI GAGTC 4 cut(s) 17, 155, 174, 423
Ppu21I YACGTR 1 cut(s) 216
Psp124BI GAGCTC 1 cut(s) 189
PspCI CACGTG 1 cut(s) 216
PspN4I GGNNCC 2 cut(s) 144, 196
PspXI VCTCGAGB 1 cut(s) 182
PvuII CAGCTG 1 cut(s) 167
RruI TCGCGA 1 cut(s) 21
RsaI GTAC 4 cut(s) 120, 314, 323, 398
RsaNI GTAC 4 cut(s) 119, 313, 322, 397
SacI GAGCTC 1 cut(s) 189
SalI GTCGAC 2 cut(s) 158, 452
SatI GCNGC 1 cut(s) 168
Sau3AI GATC 3 cut(s) 105, 261, 340
ScaI AGTACT 1 cut(s) 314
SchI GAGTC 4 cut(s) 17, 155, 174, 424
SduI GDGCHC 1 cut(s) 189
SetI ASST 6 cut(s) 169, 189, 218, 258, 378, 432
SfaNI GCATC 1 cut(s) 343
Sfr274I CTCGAG 1 cut(s) 182
SgrAI CRCCGGYG 1 cut(s) 272
SgrDI CGTCGACG 1 cut(s) 452
SlaI CTCGAG 1 cut(s) 182
SmlI CTYRAG 2 cut(s) 48, 182
SmoI CTYRAG 2 cut(s) 48, 182
SsiI CCGC 3 cut(s) 201, 250, 440
SspMI CTAG 1 cut(s) 460
SstI GAGCTC 1 cut(s) 189
TaaI ACNGT 1 cut(s) 115
TaiI ACGT 1 cut(s) 218
TaqI TCGA 5 cut(s) 108, 159, 183, 264, 453
TatI WGTACW 3 cut(s) 118, 312, 396
TseFI GTSAC 1 cut(s) 172
TseI GCWGC 1 cut(s) 167
Tsp45I GTSAC 1 cut(s) 172
TspDTI ATGAA 2 cut(s) 5, 277
XhoI CTCGAG 1 cut(s) 182
XmiI GTMKAC 2 cut(s) 159, 453
XspI CTAG 1 cut(s) 460
ZrmI AGTACT 1 cut(s) 314
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.